Tetracycline compounds

ABSTRACT

The present invention is directed to a compound represented by Structural Formula (I): 
                         
or a pharmaceutically acceptable salt thereof. The variables for Structural Formula I are defined herein.
 
     Also described is a pharmaceutical composition comprising the compound of Structural Formula I and its therapeutic use.

RELATED APPLICATIONS

This application is a continuation of U.S. application Ser. No. 13/319,298, which is the U.S. National Stage of International Application No. PCT/US2010/001350, filed May 7, 2010, which designated the United States, published in English, and claims the benefit of U.S. Provisional Application No. 61/215,757, filed on May 8, 2009. The entire teachings of the above applications are incorporated herein by reference.

BACKGROUND OF THE INVENTION

The tetracyclines are broad spectrum anti-microbial agents that are widely used in human and veterinary medicine. The total production of tetracyclines by fermentation or semi-synthesis is measured in the thousands of metric tons per year.

The widespread use of tetracyclines for therapeutic purposes has led to the emergence of resistance to these antibiotics, even among highly susceptible bacterial species. Therefore, there is need for new tetracycline analogs with improved antibacterial activities and efficacies against other tetracycline responsive diseases or disorders.

SUMMARY OF THE INVENTION

Compounds of Formula I are new tetracycline analogs with improved antibacterial activities and efficacies against other tetracycline responsive diseases or disorders:

Pharmaceutically acceptable salts of the compound of Formula I are also included. Values for the variables in Formula I are provided below:

X is selected from hydrogen, bromo, fluoro, chloro, C₁-C₆ alkyl, —O—C₁-C₆ alkyl, —S(O)_(m)—C₁-C₆ alkyl, C₃-C₇ cycloalkyl, —O—C₃-C₇ cycloalkyl, —S(O)_(m)—C₃-C₇ cycloalkyl, —CN, —N(R⁴)(R⁵), and —NH—C(O)—(C₁-C₆ alkylene)-N(R⁴)(R⁵), wherein each alkyl, alkylene or cycloalkyl in the group represented by X is optionally substituted with fluoro;

Y is selected from fluoro, —C₁-C₆ alkyl, and —[C(R^(1a))(R^(1b))]_(m)—N(R²)(R³);

Z is selected from hydrogen, fluoro, bromo, —CN, —[C(R^(1a))(R^(1b))]_(n)—N(R²)(R³), —N(R⁴)(R⁵), NO₂, —NH—C(O)—C₁-C₄ alkylene-N(R⁴)(R⁵), C₁-C₆ alkyl, —NH—C(O)—C₁-C₆ alkyl, —NH—S(O)_(m)—C₁-C₆ alkyl, —NH—S(O)_(m)—C₃-C₁₀ carbocyclyl, —NH—S(O)_(m)—(4-13 membered) heterocyclyl;

each R^(1a) and R^(1b) is independently selected from hydrogen, C₁-C₄ alkyl, and C₃-C₁₀ carbocyclyl;

R² is selected from hydrogen, C₁-C₁₂ alkyl, —C₀-C₆ alkylene-C₃-C₁₀ carbocyclyl, and —C₀-C₆ alkylene-(4-13 membered) heterocyclyl;

R³ is selected from hydrogen, C₁-C₈ alkyl, —C₀-C₆ alkylene-C₃-C₁₀ carbocyclyl, —C₀-C₆ alkylene-(4-13 membered) heterocyclyl, —C(O)—C₁-C₆ alkyl, —C₀-C₆ alkylene-C(O)N(R⁴)(R⁵), —C(O)—C₁-C₆ alkylene-N(R⁴)(R⁵), —C₂-C₆ alkylene-N(R⁴)(R⁵), —S(O)_(m)—C₁-C₆ alkyl, —S(O)_(m)—C₃-C₁₀ carbocyclyl, and —S(O)_(m)-(4-13 membered) heterocyclyl, wherein each alkyl, carbocyclyl, alkylene or heterocyclyl in the group represented by R² or R³ is optionally and independently substituted with one or more substituents independently selected from fluoro, chloro, —OH, —O—C₁-C₄ alkyl, C₁-C₄ alkyl, fluoro-substituted-C₁-C₄ alkyl, —N(R⁴)(R⁵), C₃-C₁₀ carbocyclyl or a (4-13 membered) heterocyclyl; or

R² and R³ taken together with the nitrogen atom to which they are bound form a (4-7 membered) monocyclic heterocylic ring, or a (6-13 membered) bicyclic, spirocyclic or bridged heterocylic ring, wherein the (4-7 membered) monocyclic heterocylic ring, or the (6-13 membered) bicyclic, spirocyclic or bridged heterocyclic ring optionally comprises 1 to 4 additional heteroatoms independently selected from N, S and O; and wherein the (4-7 membered) monocyclic heterocylic ring, or the (6-13 membered) bicyclic, spirocyclic or bridged heterocyclic ring is optionally substituted with one or more substituents independently selected from C₃-C₁₀ carbocyclyl, (4-13 membered) heterocyclyl, fluoro, chloro, —OH, C₁-C₄ fluoroalkyl, C₁-C₄ alkyl, —O—C₃-C₁₀ carbocyclyl, —O-(4-13 membered) heterocyclyl, —C₀-C₄ alkyl-O—C₁-C₄ alkyl, —C₀-C₄ alkyl-O—C₁-C₄ fluoroalkyl, ═O, —C(O)—C₁-C₄ alkyl, —C(O)N(R⁴)(R⁵), —N(R⁴)—C(O)—C₁-C₄ alkyl, and —C₀-C₄ alkylene-N(R⁴)(R⁵), and wherein each carbocyclyl or heterocyclyl substituent is optionally substituted with fluoro, chloro, —OH, C₁-C₄ fluoroalkyl, C₁-C₄ alkyl, —O—C₁-C₄ alkyl, —O—C₁-C₄ fluoroalkyl, —NH₂, —NH(C₁-C₄ alkyl), or —N(C₁-C₄ alkyl)₂;

each of R⁴ and R⁵ is independently selected from hydrogen and C₁-C₄ alkyl; or

R⁴ and R⁵ taken together with the nitrogen atom to which they are bound form a (4-7 membered) heterocylic ring optionally comprising one additional heteroatom selected from N, S and O, wherein the (4-7 membered) heterocylic ring is optionally substituted with fluoro, chloro, —OH, fluoro-substituted C₁-C₄ alkyl, —C₁-C₄ alkyl, or —C₁-C₄ alkylene-O—C₁-C₄ alkyl, and is optionally benzofused;

m is 0, 1 or 2; and

n is 1 or 2,

with the proviso that when Z is —NH—C(O)—C₁-C₄ alkyl-N(R⁴)(R⁵), X is other than fluoro.

Another embodiment of the present invention is directed to a pharmaceutical composition comprising a pharmaceutically acceptable carrier or diluent and a compound disclosed herein or a pharmaceutically acceptable salt thereof. The pharmaceutical composition is used in therapy, such as treating an infection in a subject.

Another embodiment of the present invention is a method of treating an infection in a subject comprising administering to the subject an effective amount of a compound disclosed herein or a pharmaceutically acceptable salt thereof.

Another embodiment of the present invention is the use of a compound disclosed herein or a pharmaceutically acceptable salt thereof for the manufacture of a medicament for treating an infection in a subject.

Another embodiment of the present invention is the use of a compound disclosed herein or a pharmaceutically acceptable salt thereof for therapy, such as treating an infection in a subject.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A-1C provide MIC values for the indicated compound.

FIGS. 2A-2K provide compounds in accordance with Structure Formula I.

FIGS. 3A-3EE provide compounds in accordance with Structure Formula I.

FIGS. 4A-4Z provide compounds in accordance with Structure Formula I.

FIGS. 5A-5O provide compounds in accordance with Structure Formula I.

FIGS. 6A-6FF provide compounds in accordance with Structure Formula I.

FIGS. 7A-7J provide MIC values for compounds of the invention.

FIGS. 8A-8D provide MIC values for compounds of the invention.

FIGS. 9A-9M provide MIC values for compounds of the invention.

FIGS. 10A-10I provide MIC values for compounds of the invention.

FIGS. 11A-11G provide MIC values for compounds of the invention.

The foregoing will be apparent from the following more particular description of example embodiments of the invention, as illustrated in the accompanying drawings in which like reference characters refer to the same parts throughout the different views. The drawings are not necessarily to scale, emphasis instead being placed upon illustrating embodiments of the present invention.

DETAILED DESCRIPTION OF THE INVENTION

The present invention is directed to a compound represented by Structural Formula I or a pharmaceutically acceptable salt thereof. Values and alternative values for the variables in Structural Formula I are defined as the following:

X is hydrogen, bromo, fluoro, chloro, C₁-C₆ alkyl, —O—C₁-C₆ alkyl, —S(O)_(m)—C₁-C₆ alkyl, C₃-C₇ cycloalkyl, —O—C₃-C₇ cycloalkyl, —S(O)_(m)—C₃-C₇ cycloalkyl, —CN, —N(R⁴)(R⁵) or —NH—C(O)—(C₁-C₆ alkylene)-N(R⁴)(R⁵). Each alkyl, alkylene or cycloalkyl in the group represented by X is optionally substituted with fluoro. In another embodiment, X is fluoro, chloro, —CN or —N(CH₃)₂. Alternatively, X is fluoro, chloro or —N(CH₃)₂. In another alternative, X is fluoro. In yet another alternative, X is chloro. In yet another alternative, X is —N(CH₃)₂. In yet another embodiment, X is hydrogen. In still another embodiment when Z is —NH—C(O)—C₁-C₄ alkyl-N(R⁴)(R⁵), X is other than fluoro.

Y is fluoro, —C₁-C₆ alkyl, or —[C(R^(1a))(R^(1b))]_(m)—N(R²)(R³). In another embodiment, Y is fluoro, methyl, —CH(R^(1a))—N(R²)(R³), —(CH₂)₂—N(R²)(R³), —NH(pyridyl), —NH—(C₁-C₈ alkyl), —NHC(O)—C₁-C₃ alkylene-piperidine, —NHC(O)—C₁-C₃ alkylene-pyrrolidine or —NHS(O)₂-phenyl, wherein each piperidine and each pyrrolidine in the group represented by Y is optionally substituted with one or more —C₁-C₆ alkyl. In another embodiment, Y is fluoro, methyl or —CH(R^(1a))—N(R²)(R³). Alternatively, Y is —CH(R^(1a))—N(R²)(R³). In another alternative, Y is fluoro. In yet another alternative, Y is —NHR³.

Z is hydrogen, fluoro, bromo, —CN, —[C(R^(1a))(R^(1b))]_(n)—N(R²)(R³), —N(R⁴)(R⁵), NO₂, —NH—C(O)—C₁-C₄ alkylene-N(R⁴)(R⁵), C₁-C₆ alkyl, —NH—C(O)—C₁-C₆ alkyl, —NH—S(O)_(m)—C₁-C₆ alkyl, —NH—S(O)_(m)—C₃-C₁₀ carbocyclyl or —NH—S(O)_(m)-(4-13 membered) heterocyclyl. In another embodiment, Z is hydrogen, NH₂ or —CH₂—NH—CH₂—C(CH₃)₃. In another embodiment, Z is hydrogen. Alternatively, Z is —[C(R^(1a))(R^(1b))]_(n)—N(R²)(R³) or —N(R⁴)(R⁵).

Each R^(1a) and R^(1b) is independently hydrogen, C₁-C₄ alkyl or C₃-C₁₀ carbocyclyl. In another alternative. R^(1a) is hydrogen or methyl.

-   -   R² is hydrogen, C₁-C₁₂ alkyl, —C₀-C₆ alkylene-C₃-C₁₀         carbocyclyl, and —C₀-C₆ alkylene-(4-13 membered) heterocyclyl.         Each alkyl, carbocyclyl, alkylene or heterocyclyl in the group         represented by R² is optionally and independently substituted         with one or more substituents independently selected from         fluoro, chloro, —OH, —O—C₁-C₄ alkyl, C₁-C₄ alkyl,         fluoro-substituted-C₁-C₄ alkyl, —N(R⁴)(R⁵), C₃-C₁₀ carbocyclyl         and a (4-13 membered) heterocyclyl. In another alternative, R²         is hydrogen, C₁-C₃ straight chained alkyl, C₁-C₃ straight         chained fluoroalkyl, cyclopropyl or —CH₂-cyclopropyl.         Alternatively, R² is hydrogen, C₁-C₃ straight chained alkyl or         —CH₂-cyclopropyl.     -   R³ is hydrogen, C₁-C₈ alkyl, —C₀-C₆ alkylene-C₃-C₁₀ carbocyclyl,         —C₀-C₆ alkylene-(4-13 membered) heterocyclyl, —C(O)—C₁-C₆ alkyl,         —C₀-C₆ alkylene-C(O)N(R⁴)(R⁵), —C(O)—C₁-C₆ alkylene-N(R⁴)(R⁵),         —C₂-C₆ alkylene-N(R⁴)(R⁵), —S(O)_(m)—C₁-C₆ alkyl,         —S(O)_(m)—C₃-C₁₀ carbocyclyl or —S(O)_(m)-(4-13 membered)         heterocyclyl. When R² is hydrogen or C₁-C₂ alkyl, R³ is         additionally benzyl. Each alkyl, carbocyclyl, alkylene or         heterocyclyl in the group represented by R³ is optionally and         independently substituted with one or more substituents         independently selected from fluoro, chloro, —O—OH, —O—C₁-C₄         alkyl, C₁-C₄ alkyl, fluoro-substituted-C₁-C₄ alkyl, —N(R⁴)(R⁵),         C₃-C₁₀ carbocyclyl and a (4-13 membered) heterocyclyl. In         another alternative, R³ is hydrogen, C₁-C₈ alkyl, —CH₂—CHF₂,         —C₂-C₆ alkylene-O—C₁-C₃ alkyl, —C₃-C₁₀ cycloalkyl, —C₃-C₁₀         cycloalkyl-substituted C₁-C₃ alkyl, cyclopropyl-substituted         cyclopropyl, —(CH₂)₂-phenyl or —S(O)₂-phenyl. Alternatively, R³         is hydrogen, C₁-C₈ alkyl, —CH₂—CHF₂, —C₁-C₆ alkylene-O—C₁-C₃         alkyl, C₃-C₁₀ cycloalkyl, C₃-C₁₀ cycloalkyl-substituted C₁-C₃         alkyl, —(CH₂)₂-phenyl, and when R² is hydrogen or —C₁-C₂ alkyl,         R³ is additionally benzyl. In another embodiment, R³ is selected         from hydrogen, C₁-C₈ alkyl, —CH₂—CHF₂, —C₁-C₆ alkylene-O—C₁-C₃         alkyl, C₃-C₁₀ cycloalkyl, —(CH₂)₂-phenyl and C₃-C₁₀         cycloalkyl-substituted C₁-C₃ alkyl, wherein each cycloalkyl in         the group represented by R³ is optionally substituted with         —C₁-C₃ alkyl or optionally benzofused.

Alternatively, R² and R³ taken together with the nitrogen atom to which they are bound form a (4-7 membered) monocyclic heterocylic ring, or a (6-13 membered) bicyclic, spirocyclic or bridged heterocylic ring, wherein the (4-7 membered) monocyclic heterocylic ring, or the (6-13 membered) bicyclic, spirocyclic or bridged heterocyclic ring optionally comprises 1 to 4 additional heteroatoms independently selected from N, S and O. The (4-7 membered) monocyclic heterocylic ring, or the (6-13 membered) bicyclic, spirocyclic or bridged heterocyclic ring is optionally substituted with one or more substituents independently selected from C₃-C₁₀ carbocyclyl, (4-13 membered) heterocyclyl, fluoro, chloro, —OH, C₁-C₄ fluoroalkyl, C₁-C₄ alkyl, —O—C₃-C₁₀ carbocyclyl, —O-(4-13 membered) heterocyclyl, —C₀-C₄ alkyl-O—C₁-C₄ alkyl, —C₀-C₄ alkyl-O—C₁-C₄ fluoroalkyl, ═O, —C(O)—C₁-C₄ alkyl, —C(O)N(R⁴)(R⁵), —N(R⁴)—C(O)—C₁-C₄ alkyl, and —C₀-C₄ alkylene-N(R⁴)(R⁵), and wherein each carbocyclyl or heterocyclyl substituent is optionally substituted with fluoro, chloro, —OH, C₁-C₄ fluoroalkyl, C₁-C₄ alkyl, —O—C₁-C₄ alkyl, —O—C₁-C₄ fluoroalkyl, —NH₂, —NH(C₁-C₄ alkyl), or —N(C₁-C₄ alkyl)₂. In another embodiment, R² and R³ taken together with the nitrogen atom to which they are bound form a ring selected from pyrrolidine, piperidine, piperazine or morpholine, wherein the ring is optionally substituted with one or more substituents independently selected from —OH, —C₁-C₃ alkyl and —C₁-C₃ alkylene-O—C₁-C₃ alkyl, and wherein the ring is optionally benzofused or spirofused to cyclopropyl. Alternatively, R² and R³ taken together with the nitrogen atom to which they are bound form a ring selected from pyrrolidine and piperidine, wherein the ring is optionally substituted with one or more substituents independently selected from fluoro, C₁-C₃ alkyl and —C₁-C₃ alkylene-O—C₁-C₃ alkyl, and wherein the ring is optionally benzofused or spirofused to cyclopropyl.

Each of R⁴ and R⁵ is independently hydrogen or C₁-C₄ alkyl.

Alternatively, R⁴ and R⁵ taken together with the nitrogen atom to which they are bound formula (4-7 membered) heterocylic ring optionally comprising one additional heteroatom selected from N, S and O, wherein the (4-7 membered) heterocylic ring is optionally substituted with fluoro, chloro, —OH, fluoro-substituted C₁-C₄ alkyl, —C₁-C₄ alkyl, or —C₁-C₄ alkylene-O—C₁-C₄ alkyl, and is optionally benzofused.

Each m is independently 0, 1 or 2.

n is 1 or 2.

In a second embodiment, the compound of the present invention is represented by Structural Formula I or a pharmaceutically acceptable salt thereof wherein, Y is fluoro, methyl, —CH(R^(1a))—N(R²)(R³), —(CH₂)₂—N(R²)(R³), —NH(pyridyl), —NH(C₁-C₈ alkyl), —NHC(O)—C₁-C₃ alkylene-piperidine, —NHC(O)—C₁-C₃ alkylene-pyrrolidine or —NHS(O)₂-phenyl, and each piperidine and each pyrrolidine in the group represented by Y is optionally substituted with one or more —C₁-C₆ alkyl; R^(1a) is hydrogen or methyl; R² is hydrogen, C₁-C₃ straight chained alkyl, C₁-C₃ straight chained fluoroalkyl, cyclopropyl or —CH₂-cyclopropyl; R³ is hydrogen, C₁-C₈ alkyl, —CH₂—CHF₂, —C₂-C₆ alkylene-O—C₁-C₃ alkyl, —C₃-C₁₀ cycloalkyl, —C₃-C₁₀ cycloalkyl-substituted C₁-C₃ alkyl, cyclopropyl-substituted cyclopropyl, —(CH₂)₂-phenyl or —S(O)₂-phenyl, and when R² is hydrogen or C₁-C₂ alkyl, R³ is additionally benzyl; or R² and R³ taken together with the nitrogen atom to which they are bound form a ring selected from pyrrolidine, piperidine, piperazine or morpholine, wherein the ring is optionally substituted with one or more substituents independently selected from —OH, —C₁-C₃ alkyl and —C₁-C₃ alkylene-O—C₁-C₃ alkyl, and wherein the ring is optionally benzofused or spirofused to cyclopropyl; and values and alternative values for the remainder of the variables are as described above.

In a third embodiment, the compound of the present invention is represented by Structural Formula I or a pharmaceutically acceptable salt thereof wherein, Y is fluoro, methyl or —CH(R^(1a))—N(R²)(R³); R^(1a) is hydrogen or methyl; R² is hydrogen, C₁-C₃ straight chained alkyl or —CH₂-cyclopropyl; R³ is hydrogen, C₁-C₈ alkyl, —CH₂—CHF₂, —C₁-C₆ alkylene-O—C₁-C₃ alkyl, C₃-C₁₀ cycloalkyl, C₃-C₁₀ cycloalkyl-substituted C₁—C₃ alkyl, wherein each cycloalkyl in the group represented by R³ is optionally substituted with —C₁-C₃ alkyl or optionally benzofused, or —(CH₂)₂-phenyl, and when R² is hydrogen or —C₁-C₂ alkyl, R³ is additionally benzyl; or R² and R³ taken together with the nitrogen atom to which they are bound form a ring selected from pyrrolidine and piperidine, wherein the ring is optionally substituted with one or more substituents independently selected from fluoro, —C₁-C₃ alkyl and —C₁-C₃ alkylene-O—C₁-C₃ alkyl, and wherein the ring is optionally benzofused or spirofused to cyclopropyl; and values and alternative values for the remainder of the variables are as described above.

In a fourth embodiment the compound of the present invention is represented by Structural Formula I or a pharmaceutically acceptable salt thereof wherein, X is fluoro, chloro, —CN or —N(CH₃)₂; Z is hydrogen, NH₂ or —CH₂—NH—CH₂—C(CH₃)₃; and values and alternative values for the remainder of the variables are as described above for Structural Formula I or for the second or third embodiment.

In another embodiment of a compound of Formula I:

X is selected from —OCH₃, —CF₃, Cl, F, and —N(CH3)₂;

-   -   Z is hydrogen and when X is F, Z is additionally selected from         —NH₂, —NH(C₁-C₂ alkyl), and —N(C₁-C₂ alkyl)₂; and

Y is —CH₂—NR²R³; wherein

-   -   R² is selected from hydrogen, and C₁-C₃ alkyl; and

R³ is selected from hydrogen, C₁-C₅ alkyl, C₀-C₆ alkylene C₃-C₁₀ carbocyclyl, C₀-C₆ alkylene-(4-13 membered) heterocyclyl, and C₂-C₆ alkylene-N(R⁴)(R⁵), wherein each carbocyclyl or heterocyclyl in the group represented by R³ is optionally and independently substituted with one or more substituents independently selected from fluoro, —OH, —O—C₁-C₃ alkyl, C₁-C₃ alkyl, fluoro-substituted C₁-C₃ alkyl, —N(R⁴)(R⁵), C₃-C₁₀ carbocyclyl or a (4-13 membered) heterocyclyl; or

R² and R³ taken together with the nitrogen atom to which they are bound form a (4-7 membered) saturated monocyclic heterocylic ring, or a (6-13 membered) saturated bicyclic, spirocyclic or bridged heterocylic ring, wherein the (4-7 membered) monocyclic heterocylic ring, or the (6-13 membered) bicyclic, spirocyclic or bridged heterocyclic ring is optionally substituted with one or more substituents independently selected from C₃-C₁₀ carbocyclyl, (4-13 membered) heterocyclyl, fluoro, —OH, —C₁-C₃ fluoroalkyl, —C₁-C₃ alkyl, O—C₃-C₁₀ carbocyclyl, O-(4-13 membered) heterocyclyl, C₀-C₂ alkylene-O—C₁-C₃ alkyl, C₀-C₂ alkylene-O—C₁-C₃ fluoroalkyl, ═O, and C₀-C₄ alkylene N(R⁴)(R⁵), and wherein each carbocyclyl or heterocyclyl substituent is optionally substituted with fluoro, —OH, C₁-C₃ fluoroalkyl, C₁-C₃ alkyl, —O—C₁-C₃ alkyl, —O—C₁-C₃ fluoroalkyl, —NH₂, —NH(C₁-C₄ alkyl), or —N(C₁-C₄ alkyl)₂; and

each of R¹ and R⁵ is independently selected from hydrogen and C₁-C₄ alkyl.

In one specific aspect of this embodiment X is —OCH₃. In another specific aspect, X is —CF₃. In still another specific aspect, X is —Cl. In another specific aspect, X is —F and Z is hydrogen. In another specific aspect, X is —F and Z is selected from —NH₂, —NH(C₁-C₂ alkyl), and —N(C₁-C₂ alkyl)₂. In still another specific aspect X is —N(CH₃)₂.

A fifth embodiment is a compound of Structural Formulas II, III, IIIa or IV or a pharmaceutically acceptable salt thereof:

Values and alternative values for the variables in Structural Formulas II, III, IIIa and IV are as defined for Structural Formula I or in the second or in the third embodiment. Alternatively, values for the variables in Structural Formula II are as defined in the fourth embodiment. In Structural Formula II, X is preferably fluoro, chloro or —N(CH₃)₂.

In a sixth embodiment, the compound of the present invention is represented by Structural Formula f or a pharmaceutically acceptable salt thereof wherein, Y is —NHR³; R³ is pyridyl, C₁-C₈ alkyl, —C(O)—C₁-C₃ alkylene-piperidine or —C(O)—C₁-C₃ alkylene-pyrrolidine. Each piperidine or pyrrolidine in the group represented by R³ is optionally substituted with one or more C₁-C₃ alkyl. Values and alternative values for the remainder of the variables are as defined for Structural Formula I above or in the second or in the third embodiment.

A seventh embodiment is a compound of Structural Formula V or a pharmaceutically acceptable salt thereof:

and values and alternative values for the remainder of the variables are as defined in the preceding paragraph.

Specific examples of compounds of the invention are represented by Structural Formula II, wherein:

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is fluoro and —CH(R^(1a))—NR²R³ is

X is chloro and —CH(R^(1a))—NR²R³ is

X is chloro and —CH(R^(1a))—NR²R³ is

X is chloro and —CH(R^(1a))—NR²R³ is

X is chloro and —CH(R^(1a))—NR²R³ is

X is chloro and —CH(R^(1a))—NR²R³ is

X is chloro and —CH(R^(1a))—NR²R³ is

X is chloro and —CH(R^(1a))—NR²R³ is

X is chloro and —CH(R^(1a))—NR²R³ is

X is chloro and —CH(R^(1a))—NR²R³ is

X is chloro and —CH(R^(1a))—NR²R³ is

X is chloro and —CH(R^(1a))—NR²R³ is

X is —N(CH₃)₂ and —CH(R^(1a))—NR²R³ is

X is —N(CH₃)₂ and —CH(R^(1a))—NR²R³ is

X is —N(CH₃)₂ and —CH(R^(1a))—NR²R³ is

X is —N(CH₃)₂ and —CH(R^(1a))—NR²R³ is

X is —N(CH₃)₂ and —CH(R^(1a))—NR²R³ is

X is —N(CH₃)₂ and —CH(R^(1a))—NR²R³ is

X is —N(CH₃)₂ and —CH(R^(1a))—NR²R³ is

X is —N(CH₃)₂ and —CH(R^(1a))—NR²R³ is

or X is —N(CH₃)₂ and —CH(R^(1a))—NR²R³ is

Other specific examples of compounds of the invention are represented by Structural Formulas IIIa, wherein:

—[C(R^(1a))(R^(1b))]_(n)—N(R²)(R³) is

Additional specific examples of compounds of the invention are represented by Structural Formula V, wherein:

R³ is

Additional specific examples of the compounds of the present invention are represented by Structural Formula IVa and IVb, or a pharmaceutically acceptable salt thereof:

Methods of preparation of the exemplified compounds are provided in Exemplification section.

Definitions

“Alkyl” means a saturated aliphatic branched or straight-chain monovalent hydrocarbon radical having the specified number of carbon atoms. Thus, “(C₁-C₁₂) alkyl” means a radical having from 1-12 carbon atoms in a linear or branched arrangement. “(C₁-C₁₂)alkyl” includes methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, undecyl and dodecyl. Unless otherwise specified, suitable substitutions for a “substituted alkyl” include halogen, —OH, —O—C₁-C₄ alkyl, C₁-C₄ alkyl, fluoro-substituted-C₁-C₄ alkyl, —O—C₁-C₄ fluoroalkyl, —NH₂, —NH(C₁-C₄ alkyl), —N(C₁-C₄ alkyl)₂, C₃-C₁₀ carbocyclyl (e.g., cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, phenyl or naphthalenyl), a (4-13 membered) heterocyclyl (e.g., pyrrolidine, piperidine, piperazine, tetrahydrofuran, tetrahydropyran or morpholine) or —N(R⁴)(R⁵), wherein R⁴ and R⁵ are as described above.

As used here, the term “alkylene” refers to a divalent alkyl group that has two points of attachment to the rest of the compound. An alkyl moiety of an alkylene group, alone or as a part of a larger moiety (alkoxy, alkylammonium, and the like) is preferably a straight chained or branched saturated aliphatic group with 1 to about 12 carbon atoms, e.g., methyl, ethyl, n-propyl, iso-propyl, n-butyl, sec-butyl, tert-butyl, pentyl, hexyl, heptyl or octyl, or a saturated cycloaliphatic group with 3 to about 12 carbon atoms. Non-limiting examples of alkylene groups include a divalent C1-6 groups such as methylene (—CH₂—), ethylene (—CH₂CH₂—), n-propylene (—CH₂CH₂CH₂—), isopropylene (—CH₂CH(CH₃)—), and the like. Examples of a divalent C1-6 alkyl group include, for example, a methylene group, an ethylene group, an ethylidene group, an n-propylene group, an isopropylene group, an isobutylene group, an s-butylene group, an n-butylene group, and a t-butylene group.

“Cycloalkyl” means a saturated aliphatic cyclic hydrocarbon radical having the specified number of carbon atoms. (C₃-C₆)cycloalkyl includes cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl. The cycloalkyl can be (3-7 membered) monocyclic, (6-13 membered) fused bicyclic, (4-13 membered) bridged bicyclic, (6-13 membered) spiro bicyclic or bridged tricyclic (e.g., adamantyl). Suitable substituents for a “substituted cycloalkyl” include, but are not limited to halogen, —OH, —O—C₁-C₄ alkyl, C₁-C₄ alkyl, fluoro-substituted-C₁-C₄ alkyl, C₃-C₁₀ carbocyclyl (e.g., cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, phenyl or naphthalenyl), a (4-13 membered) heterocyclyl (e.g., pyrrolidine, piperidine, piperazine, tetrahydrofuran, tetrahydropyran or morpholine), or —N(R⁴)(R⁵), wherein R⁴ and R⁵ are as described above.

“Heterocycle” means a saturated or partially unsaturated (4-13 membered) heterocyclic ring containing 1, 2, 3 or 4 heteroatoms independently selected from N, O or S, and includes for example heteroaryl. When one heteroatom is S, it can be optionally mono- or di-oxygenated (i.e. —S(O)— or —S(O)₂—). The heterocycle can be (4-7 membered) monocyclic, (6-13 membered) fused bicyclic, (6-13 membered) bridged bicyclic, or (6-13 membered) spiro bicyclic.

Examples of monocyclic heterocycle include, but not limited to, azetidine, pyrrolidine, piperidine, piperazine, hexahydropyrimidine, tetrahydrofuran, tetrahydropyran, morpholine, thiomorpholine, thiomorpholine 1,1-dioxide, tetrahydro-2H-1,2-thiazine, tetrahydro-2H-1,2-thiazine 1,1-dioxide, isothiazolidine, or isothiazolidine 1,1-dioxide.

A fused bicyclic heterocycle has two rings which have two adjacent ring atoms in common. The first ring is a monocyclic heterocycle and the second ring is a cycloalkyl, partially unsaturated carbocycle, phenyl or heteroaryl (e.g., pyrrole, imidazole, pyrazole, oxazole, isoxazole, thiazole, isothiazole, 1,2,3-triazole, 1,2,4-triazole, 1,3,4-oxadiazole, 1,2,5-thiadiazole, 1,2,5-thiadiazole 1-oxide, 1,2,5-thiadiazole 1,1-dioxide, 1,3,4-thiadiazole, pyridine, pyrazine, pyrimidine, pyridazine, 1,2,4-triazine, 1,3,5-triazine, and tetrazole).

A spiro bicyclic heterocycle has two rings which have only one ring atom in common. The first ring is a monocyclic heterocycle and the second ring is a cycloalkyl, partially unsaturated carbocycle or a monocyclic heterocycle. For example, the second ring is a (C₃-C₆)cycloalkyl. Example of spiro bicyclic heterocycle includes, but not limited to, azaspiro[4.4]nonane, 7-azaspiro[4.4]nonane, azaspiro[4.5]decane, 8-azaspiro[4.5]decane, azaspiro[5.5]undecane, 3-azaspiro[5.5]undecane and 3,9-di azaspiro[5.5]undecane.

A bridged bicyclic heterocycle has two rings which have three or more adjacent ring atoms in common. The first ring is a monocyclic heterocycle and the other ring is a cycloalkyl (such as (C₃-C₆)cycloalkyl), partially unsaturated carbocycle or a monocyclic heterocycle. Examples of bridged bicyclic heterocycles include, but are not limited to, azabicyclo[3.3.1]nonane, 3-azabicyclo[3.3.1]nonane, azabicyclo[3.2.1]octane, 3-azabicyclo[3.2.1]octane, 6-azabicyclo[3.2.1]octane and azabicyclo[2.2.2]octane, 2-azabicyclo[2.2.2]octane.

“Heteroaryl” means a (5-12 membered) monovalent heteroaromatic monocyclic or bicyclic ring radical. A heteroaryl contains 1, 2, 3 or 4 heteroatoms independently selected from N, O, and S. Heteroaryls include, but are not limited to pyrrole, imidazole, pyrazole, oxazole, isoxazole, thiazole, isothiazole, 1,2,3-triazole, 1,2,4-triazole, 1,3,4-oxadiazole, 1,2,5-thiadiazole, 1,2,5-thiadiazole 1-oxide, 1,2,5-thiadiazole 1,1-dioxide, 1,3,4-thiadiazole, pyridine, pyrazine, pyrimidine, pyridazine, 1,2,4-triazine, 1,3,5-triazine, and tetrazole. Bicyclic heteroaryl rings include, but are not limited to, bicyclo[4.4.0] and bicyclo[4.3.0] fused ring systems such as indolizine, indole, isoindole, indazole, benzimidazole, benzthiazole, purine, quinoline, isoquinoline, cinnoline phthalazine, quinazoline, quinoxaline, 1,8-naphthyridine, and pteridine.

“Carbocyclyl” and “carbocycle” both mean (3-10 membered) saturated, partially saturated or unsaturated aliphatic cyclic hydrocarbon ring and includes for example aryl. “Carbocyclyl” includes, but are not limited to C₃-C₆ cycloalkyl and aryl. C₃-C₆ cycloalkyl includes, but is not limited to optionally substituted cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.

“Aryl” means an aromatic monocyclic or polycyclic (e.g. bicyclic or tricyclic) carbocyclic ring system. Aryl systems include, but not limited to, phenyl, naphthalenyl, fluorenyl, indenyl, azulenyl, and anthracenyl.

“Alkoxy” means an alkyl radical attached through an oxygen linking atom. “(C₁-C₄)-alkoxy” includes methoxy, ethoxy, propoxy, and butoxy.

“Alkylthio” means an alkyl radical attached through a sulfur linking atom. “(C₁-C₄)alkylthio” include methylthio, ethylthio, propylthio and butylthio.

“Alkylsulfinyl” means an alkyl radical attached through a —S(O)— linking group. “(C₁-C₄)alkylsulfinyl” include methylsulfinyl, ethylsulfinyl, propylsulfinyl and butylsulfinyl.

“Alkylsulfonyl” means an alkyl radical attached through a —S(O)₂— linking group. “(C₁-C₄)alkylsulfonyl” include methyl sulfonyl, ethylsulfonyl, propyl sulfonyl and butylsulfonyl.

Haloalkyl and halocycloalkyl include mono, poly, and perhaloalkyl groups where each halogen is independently selected from fluorine, chlorine, and bromine.

“Cycloalkoxy” means a cycloalkyl radical attached through an oxygen linking atom. “(C₃-C₆)cycloalkoxy” includes cyclopropyloxy, cyclobutyloxy, cyclopentyloxy and cyclohexyloxy.

“Aryloxy” means an aryl moiety attached through an oxygen linking atom. Aryloxy includes, but not limited to, phenoxy.

“Arylthio” means an aryl moiety attached through a sulfur linking atom. Arylthio includes, but not limited to, phenylthio.

“Arylsulfinyl” means an aryl moiety attached through a —S(O)— linking group. Arylsulfinyl includes, but not limited to, phenylsulfinyl.

“Arylsulfonyl” means an aryl moiety attached through a —S(O)₂— linking group. Arylsulfonyl includes, but not limited to, phenylsulfonyl.

“Hetero” refers to the replacement of at least one carbon atom member in a ring system with at least one heteroatom selected from N, S, and O. A hetero ring system may have 1, 2, or 3 carbon atom members replaced by a heteroatom.

“Halogen” and “halo” are interchangeably used herein and each refers to fluorine, chlorine, bromine, or iodine.

Another embodiment of the present invention is a pharmaceutical composition comprising one or more pharmaceutically acceptable carrier and/or diluent and a compound disclosed herein or a pharmaceutically acceptable salt thereof.

“Pharmaceutically acceptable carrier” means non-therapeutic components that are of sufficient purity and quality for use in the formulation of a composition of the invention that, when appropriately administered to an animal or human, typically do not produce an adverse reaction, and that are used as a vehicle for a drug substance (i.e. a compound of the present invention).

“Pharmaceutically acceptable diluent” means non-therapeutic components that are of sufficient purity and quality for use in the formulation of a composition of the invention that, when appropriately administered to an animal or human, typically do not produce an adverse reaction, and that are used as a diluting agent for a drug substance (i.e. a compound of the present invention).

Pharmaceutically acceptable salts of the compounds of the present invention are also included. For example, an acid salt of a compound of the present invention containing an amine or other basic group can be obtained by reacting the compound with a suitable organic or inorganic acid, resulting in pharmaceutically acceptable anionic salt forms. Examples of anionic salts include the acetate, benzenesulfonate, benzoate, bicarbonate, bitartrate, bromide, calcium edetate, camsylate, carbonate, chloride, citrate, dihydrochloride, edetate, edisylate, estolate, esylate, fumarate, glyceptate, gluconate, glutamate, glycollylarsanilate, hexylresorcinate, hydrobromide, hydrochloride, hydroxynaphthoate, iodide, isethionate, lactate, lactobionate, malate, maleate, mandelate, mesylate, methylsulfate, mucate, napsylate, nitrate, pamoate, pantothenate, phosphate/diphosphate, polygalacturonate, salicylate, stearate, subacetate, succinate, sulfate, tannate, tartrate, teoclate, tosylate, and triethiodide salts.

Salts of the compounds of the present invention containing a carboxylic acid or other acidic functional group can be prepared by reacting with a suitable base. Such a pharmaceutically acceptable salt may be made with a base which affords a pharmaceutically acceptable cation, which includes alkali metal salts (especially sodium and potassium), alkaline earth metal salts (especially calcium and magnesium), aluminum salts and ammonium salts, as well as salts made from physiologically acceptable organic bases such as trimethylamine, triethylamine, morpholine, pyridine, piperidine, picoline, dicyclohexylamine, N,N′-dibenzylethylenediamine, 2-hydroxyethylamine, bis-(2-hydroxyethyl)amine, tri-(2-hydroxyethyl)amine, procaine, dibenzylpiperidine, dehydroabietylamine, N,N′-bisdehydroabietylamine, glucamine, N-methylglucamine, collidine, quinine, quinoline, and basic amino acids such as lysine and arginine.

The invention also includes various isomers and mixtures thereof. Certain of the compounds of the present invention may exist in various stereoisomeric forms. Stereoisomers are compounds which differ only in their spatial arrangement. Enantiomers are pairs of stereoisomers whose mirror images are not superimposable, most commonly because they contain an asymmetrically substituted carbon atom that acts as a chiral center. “Enantiomer” means one of a pair of molecules that are mirror images of each other and are not superimposable. Diastereomers are stereoisomers that are not related as mirror images, most commonly because they contain two or more asymmetrically substituted carbon atoms. “R” and “S” represent the configuration of substituents around one or more chiral carbon atoms. When a chiral center is not defined as R or S, a mixture of both configurations is present.

“Racemate” or “racemic mixture” means a compound of equimolar quantities of two enantiomers, wherein such mixtures exhibit no optical activity; i.e., they do not rotate the plane of polarized light.

The compounds of the invention may be prepared as individual isomers by either isomer-specific synthesis or resolved from an isomeric mixture. Conventional resolution techniques include forming the salt of a free base of each isomer of an isomeric pair using an optically active acid (followed by fractional crystallization and regeneration of the free base), forming the salt of the acid form of each isomer of an isomeric pair using an optically active amine (followed by fractional crystallization and regeneration of the free acid), forming an ester or amide of each of the isomers of an isomeric pair using an optically pure acid, amine or alcohol (followed by chromatographic separation and removal of the chiral auxiliary), or resolving an isomeric mixture of either a starting material or a final product using various well known chromatographic methods.

When the stereochemistry of a disclosed compound is named or depicted by structure, the named or depicted stereoisomer is at least 60%, 70%, 80%, 90%, 99% or 99.9% by weight pure relative to the other stereoisomers. When a single enantiomer is named or depicted by structure, the depicted or named enantiomer is at least 60%, 70%, 80%, 90%, 99% or 99.9% by weight optically pure. Percent optical purity by weight is the ratio of the weight of the enantiomer over the weight of the enantiomer plus the weight of its optical isomer.

The present invention also provides a method of treating a subject with a tetracycline-responsive disease or disorder comprising administering to the subject an effective amount of a compound of the present invention or a pharmaceutically acceptable salt thereof.

“Tetracycline-responsive disease or disorder” refers to a disease or disorder that can be treated, prevented, or otherwise ameliorated by the administration of a tetracycline compound of the present invention. Tetracycline-responsive disease or disorder includes infections, cancer, inflammatory disorders, autoimmune disease, arteriosclerosis, corneal ulceration, emphysema, arthritis, osteoporosis, osteoarthritis, multiple sclerosis, osteosarcoma, osteomyelitis, bronchiectasis, chronic pulmonary obstructive disease, skin and eye diseases, periodontitis, osteoporosis, rheumatoid arthritis, ulcerative colitis, inflammatory disorders, tumor growth and invasion, metastasis, acute lung injury, stroke, ischemia, diabetes, aortic or vascular aneurysms, skin tissue wounds, dry eye, bone, cartilage degradation, malaria, senescence, diabetes, vascular stroke, neurodegenerative disorders, cardiac disease, juvenile diabetes, acute and chronic bronchitis, sinusitis, and respiratory infections, including the common cold; acute and chronic gastroenteritis and colitis; acute and chronic cystitis and urethritis; acute and chronic dermatitis; acute and chronic conjunctivitis; acute and chronic serositis; uremic pericarditis; acute and chronic cholecystis; cystic fibrosis, acute and chronic vaginitis; acute and chronic uveitis; drug reactions; insect bites; burns and sunburn, bone mass disorder, acute lung injury, chronic lung disorders, ischemia, stroke or ischemic stroke, skin wound, aortic or vascular aneurysm, diabetic retinopathy, hemorrhagic stroke, angiogenesis, and other states for which tetracycline compounds have been found to be active (see, for example, U.S. Pat. Nos. 5,789,395; 5,834,450; 6,277,061 and 5,532,227, each of which is expressly incorporated herein by reference). Compounds of the invention can be used to prevent or control important mammalian and veterinary diseases such as diarrhea, urinary tract infections, infections of skin and skin structure, ear, nose and throat infections, wound infection, mastitis and the like. In addition, methods for treating neoplasms using tetracycline compounds of the invention are also included (van der Bozert et al., Cancer Res., 48: 6686-6690 (1988)).

Infections that can be treated using compounds of the invention (i.e. compound of Structural Formula (I)) or a pharmaceutically acceptable salt thereof include, but are not limited to, skin infections, GI infections, urinary tract infections, genito-urinary infections, respiratory tract infections, sinuses infections, middle ear infections, systemic infections, cholera, influenza, bronchitis, acne, malaria, sexually transmitted disease including syphilis and gonorrhea, Legionnaires' disease, Lyme disease, Rocky Mountain spotted fever, Q fever, typhus, bubonic plague, gas gangrene, hospital acquired infections, leptospirosis, whooping cough, anthrax and infections caused by the agents responsible for lymphogranuloma venereum, inclusion conjunctivitis, or psittacosis. Infections can be bacterial, fungal, parasitic and viral infections (including those which are resistant to other tetracycline compounds).

In one embodiment, the infection can be caused bacteria. In another embodiment, the infection is caused by a Gram-positive bacteria. In a specific aspect of this embodiment, the infection is caused by a Gram-positive bacteria selected from S. aureus, S. pneumoniae, P. granulosum and P. acnes.

In another embodiment, the infection is caused by a Gram-negative bacteria. In a specific aspect of this embodiment, the infection is caused by a Gram-negative bacteria selected from E. coli or B. thetaiotaomicron.

In another embodiment, the infection is caused by an organism selected from the group consisting of K. pneumoniae, Salmonella, E. hirae, A. baumanii, B. catarrhalis, H. influenzae, P. aeruginosa, E. faecium, E. coli, S. aureus, and E. faecalis. In another embodiment, the infection is caused by an organism selected from the group consisting of rickettsiae, chlamydiae, and Mycoplasma pneumoniae. In another embodiment, the infection is caused by an organism resistant to tetracycline. In another embodiment, the infection is caused by an organism resistant to methicillin. In another embodiment, the infection is caused by an organism resistant to vancomycin. In another embodiment the infection is a Bacillus anthracis infection. “Bacillus anthracis infection” includes any state, diseases, or disorders caused or which result from exposure or alleged exposure to Bacillus anthracis or another member of the Bacillus cereus group of bacteria.

In a further embodiment, the tetracycline responsive disease or disorder is not a bacterial infection. In another embodiment, the tetracycline compounds of the invention are essentially non-antibacterial. For example, non-antibacterial compounds of the invention may have MIC values greater than about 4 μg/ml (as measured by assays known in the art and/or the assay given in Example 22).

Tetracycline responsive disease or disorder also includes diseases or disorders associated with inflammatory process associated states (IPAS). The term “inflammatory process associated state” includes states in which inflammation or inflammatory factors (e.g., matrix metalloproteinases (MMPs), nitric oxide (NO), TNF, interleukins, plasma proteins, cellular defense systems, cytokines, lipid metabolites, proteases, toxic radicals, adhesion molecules, etc.) are involved or are present in an area in aberrant amounts, e.g., in amounts which may be advantageous to alter, e.g., to benefit the subject. The inflammatory process is the response of living tissue to damage. The cause of inflammation may be due to physical damage, chemical substances, micro-organisms, tissue necrosis, cancer or other agents. Acute inflammation is short-lasting, lasting only a few days. If it is longer lasting however, then it may be referred to as chronic inflammation.

IPASs include inflammatory disorders. Inflammatory disorders are generally characterized by heat, redness, swelling, pain and loss of function. Examples of causes of inflammatory disorders include, but are not limited to, microbial infections (e.g., bacterial and fungal infections), physical agents (e.g., burns, radiation, and trauma), chemical agents (e.g., toxins and caustic substances), tissue necrosis and various types of immunologic reactions.

Examples of inflammatory disorders can be treated using the compounds of the invention (i.e. compound of Structural Formula (I)) or a pharmaceutically acceptable salt thereof include, but are not limited to, osteoarthritis, rheumatoid arthritis, acute and chronic infections (bacterial and fungal, including diphtheria and pertussis); acute and chronic bronchitis, sinusitis, and upper respiratory infections, including the common cold; acute and chronic gastroenteritis and colitis; inflammatory bowel disorder; acute and chronic cystitis and urethritis; vasculitis; sepsis; nephritis; pancreatitis; hepatitis; lupus; inflammatory skin disorders including, for example, eczema, dermatitis, psoriasis, pyoderma gangrenosum, acne rosacea, and acute and chronic dermatitis; acute and chronic conjunctivitis; acute and chronic serositis (pericarditis, peritonitis, synovitis, pleuritis and tendinitis); uremic pericarditis; acute and chronic cholecystis; acute and chronic vaginitis; acute and chronic uveitis; drug reactions; insect bites; burns (thermal, chemical, and electrical); and sunburn.

IPASs also include matrix metalloproteinase associated states (MMPAS). MMPAS include states characterized by aberrant amounts of MMPs or MMP activity. Examples of matrix metalloproteinase associated states (“MMPAS's”) can be treated using compounds of the invention or a pharmaceutically acceptable salt thereof, include, but are not limited to, arteriosclerosis, corneal ulceration, emphysema, osteoarthritis, multiple sclerosis (Liedtke et al., Ann. Neurol. 1998, 44: 35-46; Chandler et al., J. Neuroimmunol. 1997, 72: 155-71), osteosarcoma, osteomyelitis, bronchiectasis, chronic pulmonary obstructive disease, skin and eye diseases, periodontitis, osteoporosis, rheumatoid arthritis, ulcerative colitis, inflammatory disorders, tumor growth and invasion (Stetler-Stevenson et al., Annu. Rev. Cell Biol. 1993, 9: 541-73; Tryggvason et al., Biochim. Biophys. Acta 1987, 907: 191-217; Li et al., Mol. Carcillog 1998, 22: 84-89)), metastasis, acute lung injury, stroke, ischemia, diabetes, aortic or vascular aneurysms, skin tissue wounds, dry eye, bone and cartilage degradation (Greenwald et al., Bone 1998, 22: 33-38; Ryan et al., Curr. Op. Rheumatol. 1996, 8: 238-247). Other MMPAS include those described in U.S. Pat. Nos. 5,459,135; 5,321,017; 5,308,839; 5,258,371; 4,935,412; 4,704,383, 4,666,897, and RE 34,656, incorporated herein by reference in their entirety.

In a further embodiment, the IPAS includes disorders described in U.S. Pat. Nos. 5,929,055; and 5,532,227, incorporated herein by reference in their entirety.

Tetracycline responsive disease or disorder also includes diseases or disorders associated with NO associated states. The term “NO associated states” includes states which involve or are associated with nitric oxide (NO) or inducible nitric oxide synthase (iNOS). NO associated state includes states which are characterized by aberrant amounts of NO and/or iNOS. Preferably, the NO associated state can be treated by administering tetracycline compounds of the invention. The disorders, diseases and states described in U.S. Pat. Nos. 6,231,894; 6,015,804; 5,919,774; and 5,789,395 are also included as NO associated states. The entire contents of each of these patents are hereby incorporated herein by reference.

Examples of diseases or disorders associated with NO associated states can be treated using the compounds of the present invention (i.e. compound of Structural Formula (I)) or a pharmaceutically acceptable salt thereof include, but are not limited to, malaria, senescence, diabetes, vascular stroke, neurodegenerative disorders (Alzheimer's disease and Huntington's disease), cardiac disease (reperfusion-associated injury following infarction), juvenile diabetes, inflammatory disorders, osteoarthritis, rheumatoid arthritis, acute, recurrent and chronic infections (bacterial, viral and fungal); acute and chronic bronchitis, sinusitis, and respiratory infections, including the common cold; acute and chronic gastroenteritis and colitis; acute and chronic cystitis and urethritis; acute and chronic dermatitis; acute and chronic conjunctivitis; acute and chronic serositis (pericarditis, peritonitis, synovitis, pleuritis and tendonitis); uremic pericarditis; acute and chronic cholecystis; cystic fibrosis, acute and chronic vaginitis; acute and chronic uveitis; drug reactions; insect bites; burns (thermal, chemical, and electrical); and sunburn.

In another embodiment, the tetracycline responsive disease or disorder is cancer. Examples of cancers that can be treated using the compounds of the invention (i.e. compound of Structural Formula (I)) or a pharmaceutically acceptable salt thereof include all solid tumors, i.e., carcinomas e.g., adenocarcinomas, and sarcomas. Adenocarcinomas are carcinomas derived from glandular tissue or in which the tumor cells form recognizable glandular structures. Sarcomas broadly include tumors whose cells are embedded in a fibrillar or homogeneous substance like embryonic connective tissue. Examples of carcinomas which may be treated using the methods of the invention include, but are not limited to, carcinomas of the prostate, breast, ovary, testis, lung, colon, and breast. The methods of the invention are not limited to the treatment of these tumor types, but extend to any solid tumor derived from any organ system. Examples of treatable cancers include, but are not limited to, colon cancer, bladder cancer, breast cancer, melanoma, ovarian carcinoma, prostate carcinoma, lung cancer, and a variety of other cancers as well. The methods of the invention also cause the inhibition of cancer growth in adenocarcinomas, such as, for example, those of the prostate, breast, kidney, ovary, testes, and colon. In one embodiment, the cancers treated by methods of the invention include those described in U.S. Pat. Nos. 6,100,248; 5,843,925; 5,837,696; or 5,668,122, incorporated herein by reference in their entirety.

Alternatively, the tetracycline compounds may be useful for preventing or reducing the likelihood of cancer recurrence, for example, to treat residual cancer following surgical resection or radiation therapy. The tetracycline compounds useful according to the invention are especially advantageous as they are substantially non-toxic compared to other cancer treatments.

In a further embodiment, the compounds of the invention are administered in combination with standard cancer therapy, such as, but not limited to, chemotherapy.

Examples of tetracycline responsive states can be treated using the compounds of the invention (i.e. compound of Structural Formula (I)) or a pharmaceutically acceptable salt thereof also include neurological disorders which include both neuropsychiatric and neurodegenerative disorders, but are not limited to, such as Alzheimer's disease, dementias related to Alzheimer's disease (such as Pick's disease), Parkinson's and other Lewy diffuse body diseases, senile dementia, Huntington's disease, Gilles de la Tourette's syndrome, multiple sclerosis, amyotrophic lateral sclerosis (ALS), progressive supranuclear palsy, epilepsy, and Creutzfeldt-Jakob disease; autonomic function disorders such as hypertension and sleep disorders, and neuropsychiatric disorders, such as depression, schizophrenia, schizoaffective disorder, Korsakoffs psychosis, mania, anxiety disorders, or phobic disorders; learning or memory disorders, e.g., amnesia or age-related memory loss, attention deficit disorder, dysthymic disorder, major depressive disorder, mania, obsessive-compulsive disorder, psychoactive substance use disorders, anxiety, phobias, panic disorder, as well as bipolar affective disorder, e.g., severe bipolar affective (mood) disorder (BP-1), bipolar affective neurological disorders, e.g., migraine and obesity.

Further neurological disorders include, for example, those listed in the American Psychiatric Association's Diagnostic and Statistical manual of Mental Disorders (DSM), the most current version of which is incorporated herein by reference in its entirety.

In another embodiment, the tetracycline responsive disease or disorder is diabetes. Diabetes that can be treated using the compounds of the invention or a pharmaceutically acceptable salt thereof include, but are not limited to, juvenile diabetes, diabetes mellitus, diabetes type I, or diabetes type II. In a further embodiment, protein glycosylation is not affected by the administration of the tetracycline compounds of the invention. In another embodiment, the tetracycline compound of the invention is administered in combination with standard diabetic therapies, such as, but not limited to insulin therapy.

In another embodiment, the tetracycline responsive disease or disorder is a bone mass disorder. Bone mass disorders that can be treated using the compounds of the invention or a pharmaceutically acceptable salt thereof include disorders where a subjects bones are disorders and states where the formation, repair or remodeling of bone is advantageous. For examples bone mass disorders include osteoporosis (e.g., a decrease in bone strength and density), bone fractures, bone formation associated with surgical procedures (e.g., facial reconstruction), osteogenesis imperfecta (brittle bone disease), hypophosphotasia, Paget's disease, fibrous dysplasia, osteopetrosis, myeloma bone disease, and the depletion of calcium in bone, such as that which is related to primary hyperparathyroidism. Bone mass disorders include all states in which the formation, repair or remodeling of bone is advantageous to the subject as well as all other disorders associated with the bones or skeletal system of a subject which can be treated with the tetracycline compounds of the invention. In a further embodiment, the bone mass disorders include those described in U.S. Pat. Nos. 5,459,135; 5,231,017; 5,998,390; 5,770,588; RE 34,656; 5,308,839; 4,925,833; 3,304,227; and 4,666,897, each of which is hereby incorporated herein by reference in its entirety.

In another embodiment, the tetracycline responsive disease or disorder is acute lung injury. Acute lung injuries that can be treated using the compounds of the invention or a pharmaceutically acceptable salt thereof include adult respiratory distress syndrome (ARDS), post-pump syndrome (PPS), and trauma. Trauma includes any injury to living tissue caused by an extrinsic agent or event. Examples of trauma include, but are not limited to, crush injuries, contact with a hard surface, or cutting or other damage to the lungs.

The tetracycline responsive disease or disorder of the invention also includes chronic lung disorders. Examples of chronic lung disorders that can be treated using the compounds of the invention or a pharmaceutically acceptable salt thereof include, but are not limited, to asthma, cystic fibrosis, chronic obstructive pulmonary disease (COPD), and emphysema. In a further embodiment, the acute and/or chronic lung disorders that can be treated using the compounds of the invention or a pharmaceutically acceptable salt thereof include those described in U.S. Pat. Nos. 5,977,091; 6,043,231; 5,523,297; and 5,773,430, each of which is hereby incorporated herein by reference in its entirety.

In yet another embodiment, the tetracycline responsive disease or disorder is ischemia, stroke, or ischemic stroke.

In a further embodiment, the tetracycline compounds of the invention or a pharmaceutically acceptable salt thereof can be used to treat such disorders as described above and in U.S. Pat. Nos. 6,231,894; 5,773,430; 5,919,775 and 5,789,395, incorporated herein by reference.

In another embodiment, the tetracycline responsive disease or disorder is a skin wound. The invention also provides a method for improving the healing response of the epithelialized tissue (e.g., skin, mucosae) to acute traumatic injury (e.g., cut, burn, scrape, etc.). The method includes using a tetracycline compound of the invention or a pharmaceutically acceptable salt thereof to improve the capacity of the epithelialized tissue to heal acute wounds. The method may increase the rate of collagen accumulation of the healing tissue. The method may also decrease the proteolytic activity in the epithelialized tissue by decreasing the collagenolytic and/or gellatinolytic activity of MMPs. In a further embodiment, the tetracycline compound of the invention or a pharmaceutically acceptable salt thereof is administered to the surface of the skin (e.g., topically). In a further embodiment, the tetracycline compound of the invention or a pharmaceutically acceptable salt thereof is used to treat a skin wound, and other such disorders as described in, for example, U.S. Pat. Nos. 5,827,840; 4,704,383; 4,935,412; 5,258,371; 5,308,839, 5,459,135; 5,532,227; and 6,015,804; each of which is incorporated herein by reference in its entirety.

In yet another embodiment, the tetracycline responsive disease or disorder is an aortic or vascular aneurysm in vascular tissue of a subject (e.g., a subject having or at risk of having an aortic or vascular aneurysm, etc.). The tetracycline compound or a pharmaceutically acceptable salt thereof may be effective to reduce the size of the vascular aneurysm or it may be administered to the subject prior to the onset of the vascular aneurysm such that the aneurysm is prevented. In one embodiment, the vascular tissue is an artery, e.g., the aorta, e.g., the abdominal aorta. In a further embodiment, the tetracycline compounds of the invention are used to treat disorders described in U.S. Pat. Nos. 6,043,225 and 5,834,449, incorporated herein by reference in their entirety.

The compounds of the invention or a pharmaceutically acceptable salt thereof can be used alone or in combination with one or more therapeutic agent in the methods of the invention disclosed herein.

The language “in combination with” another therapeutic agent or treatment includes co-administration of the tetracycline compound and with the other therapeutic agent or treatment as either a single combination dosage form or as multiple, separate dosage forms, administration of the tetracycline compound first, followed by the other therapeutic agent or treatment and administration of the other therapeutic agent or treatment first, followed by the tetracycline compound.

The other therapeutic agent may be any agent that is known in the art to treat, prevent, or reduce the symptoms of a tetracycline-responsive disease or disorder. The choice of additional therapeutic agent(s) is based upon the particular tetracycline-responsive disease or disorder being treated. Such choice is within the knowledge of a treating physician. Furthermore, the other therapeutic agent may be any agent of benefit to the patient when administered in combination with the administration of a tetracycline compound.

As used herein, the term “subject” means a mammal in need of treatment, e.g., companion animals (e.g., dogs, cats, and the like), farm animals (e.g., cows, pigs, horses, sheep, goats and the like) and laboratory animals (e.g., rats, mice, guinea pigs and the like). Typically, the subject is a human in need of the specified treatment.

As used herein, the term “treating” or “treatment” refers to obtaining desired pharmacological and/or physiological effect. The effect can be prophylactic or therapeutic, which includes achieving, partially or substantially, one or more of the following results: partially or totally reducing the extent of the disease, disorder or syndrome; ameliorating or improving a clinical symptom or indicator associated with the disorder; delaying, inhibiting or decreasing the likelihood of the progression of the disease, disorder or syndrome; or partially or totally delaying, inhibiting or reducing the likelihood of the onset or development of disease, disorder or syndrome.

“Effective amount” means that amount of active compound agent that elicits the desired biological response in a subject. Such response includes alleviation of the symptoms of the disease or disorder being treated. In one embodiment, the effective amount of a compound of the invention is from about 0.01 mg/kg/day to about 1000 mg/kg/day, from about 0.1 mg/kg/day to about 100 mg/kg/day, or from about 0.5 mg/kg/day to about 50 mg/kg/day.

The invention further includes the process for making the composition comprising mixing one or more of the present compounds and an optional pharmaceutically acceptable carrier; and includes those compositions resulting from such a process, which process includes conventional pharmaceutical techniques.

The compositions of the invention include ocular, oral, nasal, transdermal, topical with or without occlusion, intravenous (both bolus and infusion), and injection (intraperitoneally, subcutaneously, intramuscularly, intratumorally, or parenterally). The composition may be in a dosage unit such as a tablet, pill, capsule, powder, granule, liposome, ion exchange resin, sterile ocular solution, or ocular delivery device (such as a contact lens and the like facilitating immediate release, timed release, or sustained release), parenteral solution or suspension, metered aerosol or liquid spray, drop, ampoule, auto-injector device, or suppository; for administration ocularly, orally, intranasally, sublingually, parenterally, or rectally, or by inhalation or insufflation.

Compositions of the invention suitable for oral administration include solid forms such as pills, tablets, caplets, capsules (each including immediate release, timed release, and sustained release formulations), granules and powders; and, liquid forms such as solutions, syrups, elixirs, emulsions, and suspensions. Forms useful for ocular administration include sterile solutions or ocular delivery devices. Forms useful for parenteral administration include sterile solutions, emulsions, and suspensions.

The compositions of the invention may be administered in a form suitable for once-weekly or once-monthly administration. For example, an insoluble salt of the active compound may be adapted to provide a depot preparation for intramuscular injection (e.g., a decanoate salt) or to provide a solution for ophthalmic administration.

The dosage form containing the composition of the invention contains an effective amount of the active ingredient necessary to provide a therapeutic effect. The composition may contain from about 5,000 mg to about 0.5 mg (preferably, from about 1,000 mg to about 0.5 mg) of a compound of the invention or salt form thereof and may be constituted into any form suitable for the selected mode of administration. The composition may be administered about 1 to about 5 times per day. Daily administration or post-periodic dosing may be employed.

For oral administration, the composition is preferably in the form of a tablet or capsule containing, e.g., 500) to 0.5 milligrams of the active compound. Dosages will vary depending on factors associated with the particular patient being treated (e.g., age, weight, diet, and time of administration), the severity of the condition being treated, the compound being employed, the mode of administration, and the strength of the preparation.

The oral composition is preferably formulated as a homogeneous composition, wherein the active ingredient is dispersed evenly throughout the mixture, which may be readily subdivided into dosage units containing equal amounts of a compound of the invention. Preferably, the compositions are prepared by mixing a compound of the invention (or pharmaceutically acceptable salt thereof) with one or more optionally present pharmaceutical carriers (such as a starch, sugar, diluent, granulating agent, lubricant, glidant, binding agent, and disintegrating agent), one or more optionally present inert pharmaceutical excipients (such as water, glycols, oils, alcohols, flavoring agents, preservatives, coloring agents, and syrup), one or more optionally present conventional tableting ingredients (such as corn starch, lactose, sucrose, sorbitol, talc, stearic acid, magnesium stearate, dicalcium phosphate, and any of a variety of gums), and an optional diluent (such as water).

Binder agents include starch, gelatin, natural sugars (e.g., glucose and beta-lactose), corn sweeteners and natural and synthetic gums (e.g., acacia and tragacanth). Disintegrating agents include starch, methyl cellulose, agar, and bentonite.

Tablets and capsules represent an advantageous oral dosage unit form. Tablets may be sugarcoated or filmcoated using standard techniques. Tablets may also be coated or otherwise compounded to provide a prolonged, control-release therapeutic effect. The dosage form may comprise an inner dosage and an outer dosage component, wherein the outer component is in the form of an envelope over the inner component. The two components may further be separated by a layer which resists disintegration in the stomach (such as an enteric layer) and permits the inner component to pass intact into the duodenum or a layer which delays or sustains release. A variety of enteric and non-enteric layer or coating materials (such as polymeric acids, shellacs, acetyl alcohol, and cellulose acetate or combinations thereof) may be used.

Compounds of the invention may also be administered via a slow release composition; wherein the composition includes a compound of the invention and a biodegradable slow release carrier (e.g., a polymeric carrier) or a pharmaceutically acceptable non-biodegradable slow release carrier (e.g., an ion exchange carrier).

Biodegradable and non-biodegradable slow release carriers are well known in the art. Biodegradable carriers are used to form particles or matrices which retain an active agent(s) and which slowly degrade/dissolve in a suitable environment (e.g., aqueous, acidic, basic and the like) to release the agent. Such particles degrade/dissolve in body fluids to release the active compound(s) therein. The particles are preferably nanoparticles (e.g., in the range of about 1 to 500 nm in diameter, preferably about 50-200 nm in diameter, and most preferably about 100 nm in diameter). In a process for preparing a slow release composition, a slow release carrier and a compound of the invention are first dissolved or dispersed in an organic solvent. The resulting mixture is added into an aqueous solution containing an optional surface-active agent(s) to produce an emulsion. The organic solvent is then evaporated from the emulsion to provide a colloidal suspension of particles containing the slow release carrier and the compound of the invention.

The compound disclosed herein may be incorporated for administration orally or by injection in a liquid form such as aqueous solutions, suitably flavored syrups, aqueous or oil suspensions, flavored emulsions with edible oils such as cottonseed oil, sesame oil, coconut oil or peanut oil and the like, or in elixirs or similar pharmaceutical vehicles. Suitable dispersing or suspending agents for aqueous suspensions, include synthetic and natural gums such as tragacanth, acacia, alginate, dextran, sodium carboxymethylcellulose, methylcellulose, polyvinyl-pyrrolidone, and gelatin. The liquid forms in suitably flavored suspending or dispersing agents may also include synthetic and natural gums. For parenteral administration, sterile suspensions and solutions are desired. Isotonic preparations, which generally contain suitable preservatives, are employed when intravenous administration is desired.

The compounds may be administered parenterally via injection. A parenteral formulation may consist of the active ingredient dissolved in or mixed with an appropriate inert liquid carrier. Acceptable liquid carriers usually comprise aqueous solvents and other optional ingredients for aiding solubility or preservation. Such aqueous solvents include sterile water, Ringer's solution, or an isotonic aqueous saline solution. Other optional ingredients include vegetable oils (such as peanut oil, cottonseed oil, and sesame oil), and organic solvents (such as solketal, glycerol, and formyl). A sterile, non-volatile oil may be employed as a solvent or suspending agent. The parenteral formulation is prepared by dissolving or suspending the active ingredient in the liquid carrier whereby the final dosage unit contains from 0.005 to 10% by weight of the active ingredient. Other additives include preservatives, isotonizers, solubilizers, stabilizers, and pain-soothing agents. Injectable suspensions may also be prepared, in which case appropriate liquid carriers, suspending agents and the like may be employed.

Compounds of the invention may be administered intranasally using a suitable intranasal vehicle.

Compounds of the invention may also be administered topically using a suitable topical transdermal vehicle or a transdermal patch.

For ocular administration, the composition is preferably in the form of an ophthalmic composition. The ophthalmic compositions are preferably formulated as eye-drop formulations and filled in appropriate containers to facilitate administration to the eye, for example a dropper fitted with a suitable pipette. Preferably, the compositions are sterile and aqueous based, using purified water. In addition to the compound of the invention, an ophthalmic composition may contain one or more of: a) a surfactant such as a polyoxyethylene fatty acid ester; b) a thickening agents such as cellulose, cellulose derivatives, carboxyvinyl polymers, polyvinyl polymers, and polyvinylpyrrolidones, typically at a concentration n the range of about 0.05 to about 5.0% (wt/vol); c) (as an alternative to or in addition to storing the composition in a container containing nitrogen and optionally including a free oxygen absorber such as Fe), an anti-oxidant such as butylated hydroxyanisol, ascorbic acid, sodium thiosulfate, or butylated hydroxytoluene at a concentration of about 0.00005 to about 0.1% (wt/vol); d) ethanol at a concentration of about 0.01 to 0.5% (wt/vol); and e) other excipients such as an isotonic agent, buffer, preservative, and/or pH-controlling agent. The pH of the ophthalmic composition is desirably within the range of 4 to 8.

In certain embodiments, the composition of this invention includes one or more additional agents. The other therapeutic agent may be a agent that is capable of treating, preventing or reducing the symptoms of a tetracycline-responsive disease or disorder. Alternatively, the other therapeutic agent may be any agent of benefit to a patient when administered in combination with the tetracycline compound in this invention.

The following abbreviations are used in the synthesis examples below.

Abbreviations

Ac acetyl

AIBN 2,2′-azobis(2-methylpropionitrile)

aq aqueous

Bn benzyl

Boc tert-butoxycarbonyl

Bu butyl

Cbz benzyl oxycarbonyl

Cy tricyclohexylphosphine

dba dibenzylideneacetone

DIBAL-H diisobutylaluminum hydride

DIEA N,N-diisopropylethylamine

DMAP 4-(dimethylamino)pyridine

DME 1,2-dimethoxyethane

DMF N,N-dimethylformamide

DMPU 1,3-dimethyl-3,4-5,6-tetrahydro-2(1H)-pyrimidone

DMSO dimethyl sulfoxide

EDC N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide

ESI electrospray ionization

Et ethyl

EtOAc ethyl acetate

HPLC high performance liquid chromatography

HOBt 1-hydroxybenzotriazole

i iso

IBX 2-iodoxybenzoic acid

LDA lithium diisopropylamide

LHMDS lithium bis(trimethylsilyl)amide

LTMP lithium 2,2,6,6-tetramethylpiperidide

MeOH methanol

Ms methanesulfonyl

MS mass spectrometry

MTBE methyl tert-butyl ether

NBS N-bromosuccinimide

NCS N-chlorosuccimide

NMR nuclear magnetic resonance spectrometry

Ph phenyl

Pr propyl

s secondary

t tertiary

TMEDA N,N,N′N′-tetramethylethylenediamine

TBS tert-butyldimethyl silyl

TEA tri ethylamine

Tf trifluoromethanesulfonyl

TFA trifluoroacetic acid

TFAA trifluoroacetic anhydride

THF tetrahydrofuran

TLC thin layer chromatography

Ts para-toluenesulfonyl

TsOH para-toluenesulfonic acid

Xantphos 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene

Example 1. Synthesis of Compounds Via Scheme 1

The following compounds were prepared according to Scheme 1.

To a THF solution of 5-fluoro-2-methoxybenzoic acid (S1-1, 0.50 g, 2.94 mmol, 1.0 equiv, Aldrich 523097) cooled at −78° C. was added a THF solution of s-BuLi (4.60 mL, 1.40 M/cyclohexane, 6.44 mmol, 2.2 equiv) and TMEDA (0.97 mL, 6.47 mmol, 2.2 equiv). The reaction was stirred at −78° C. for 2 hrs. Iodomethane (1.10 mL, 17.64 mmol, 6.0 equiv) was added to the reaction mixture dropwise. The reaction was allowed to warm to 25° C. over 1 h and stirred at 25° C. for 1 h. Aqueous NaOH (6 N, 20 mL) was added. The resulting mixture was extracted with t-butylmethyl ether (20 mL×2). The aqueous layer was acidified with hydrochloric acid (6 N) to pH 1 and extracted with EtOAc (20 mL×4). The combined EtOAc extracts were dried over sodium sulfate and concentrated to give 0.51 g of crude S1-2: ¹H NMR (400 MHz, CDCl₃) δ 7.06 (dd, J=9.8, 8.5 Hz, 1H), 6.75 (dd, J=9.8, 3.7 Hz, 1H), 3.86 (s, 3H), 2.34 (d, J=2.4 Hz, 3H); MS (ESI) m/z 185.12 (M+H).

Oxalyl chloride (0.95 mL, 11.10 mmol, 5.5 equiv) was added to a dichloromethane solution (15 mL, anhydrous) of S1-2 (0.51 g, 2.00 mmol, 1.0 equiv). DMF (0.1 mL) was added to the resulting mixture. The reaction was stirred at 25° C. for 1 h and concentrated. The resulting solid was redissolved in 15 mL of anhydrous dichloromethane Phenol (0.52 g, 5.50 mmol, 2.8 equiv), DMAP (0.67 g, 5.60 mmol, 2.8 equiv), and triethylamine (1.90 mL, 13.90 mmol, 7.0 equiv) were added to the reaction mixture. The reaction was stirred at 25° C. for 12 hrs and concentrated. EtOAc and H₂O were added to the residue. The organic layer was washed with aqueous NaOH (1 N), H₂O, and brine, dried over sodium sulfate, and concentrated. Flash chromatography on silica gel (40:1 hexanes/EtOAc) yielded 0.40 g of compound S1-3 (52%, 2 steps): ¹H NMR (400 MHz, CDCl₃) δ 7.47-7.41 (m, 2H), 7.31-7.24 (m, 3H), 7.08 (dd, J=9.2, 9.2 Hz, 1H), 6.77 (dd, J=9.2, 3.7 Hz, 1H), 3.88 (s, 3H), 2.36 (d, J=2.3 Hz, 3H); MS (ESI) m/z 261.12 (M+H).

BBr₃ (1.85 mL, 1 M/dichloromethane, 1.85 mmol, 1.2 equiv) was added to a dichloromethane solution (8 mL) of S1-3 (0.40 g, 1.54 mmol, 1.0 equiv) at −78° C. The reaction was stirred from −78° C. to 25° C. for 1.5 hrs, quenched by saturated aqueous NaHCO₃, and concentrated. EtOAc and H₂O were added to the reaction mixture. The aqueous layer was extracted with EtOAc. The combined Et(O)Ac extracts were dried over sodium sulfate and concentrated under reduced pressure to yield 0.36 g of crude S1-4: ¹H NMR (400 MHz, CDCl₃) δ 10.66 (s, 1H), 7.50-7.44 (m, 2H), 7.36-7.31 (m, 1H), 7.26-7.18 (m, 3H), 6.86 (dd, J=9.3, 4.9 Hz, 1H), 2.60 (d, J=2.4 Hz, 3H); MS (ESI) m/z 245.11 (M−H).

Compound S1-4 (4.92 g, 95% purity, 20.00 mmol, 1.0 equiv) was dissolved in acetic acid (50 mL). Bromine (1.54 mL, 30.00 mmol, 1.5 equiv) was added via syringe at rt. After stirring at rt for 2 hrs, LC/MS indicated that the starting material was consumed. The reaction mixture was diluted with EtOAc, washed with water (3×100 mL) and brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure to give 7.06 g of compound S1-5 as a light yellow solid: ¹H NMR (400 MHz, CDCl₃) δ 11.14 (s, 1H), 7.52 (d, J=9.2 Hz, 1H), 7.49-7.43 (m, 2H), 7.36-7.30 (m, 1H), 7.21-7.16 (m, 2H), 2.55 (d, J=2.3 Hz, 3H).

Compound S1-5 (crude, 1.06 g, 2.97 mmol, 1.0 equiv) was dissolve in acetone (20 mL), added with potassium carbonate (0.82 g, 5.94 mmol, 2.0 equiv), and cooled to 0° C. in an ice-bath. Benzyl bromide (540 μL, 4.45 mmol, 1.5 equiv) was added dropwise. After 2 hrs, LC/MS indicated that 40% of the starting material was consumed. The reaction mixture was heated to 50° C. for another hour and the starting material was completely consumed. The reaction mixture was diluted with EtOAc (100 mL), washed with water and brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure to give 2.2 g of crude S1-6, which was purified by column chromatography (Biotage 10 g column, 2 to 5% EtOAc in hexanes gradient), yielding 1.03 g (84%, two steps) of the pure compound S1-6 as an colorless oil: ¹H NMR (400 MHz, CDCl₃) δ 7.50-7.47 (m, 2H), 7.40-7.33 (m, 6H), 7.25 (t, J=7.3 Hz, 1H), 7.04 (d, J=8.6 Hz, 2H), 5.09 (s, 2H), 2.32 (d, J=1.8 Hz, 3H).

An LDA/THF solution was prepared by adding n-BuLi (1.6 M/hexanes, 5.10 mL, 8.16 mmol, 1.5 equiv) to diisopropylamine (1.15 mL, 8.16 mmol, 1.5 equiv) in THF (15 mL) at −78° C. The reaction mixture was warmed to −20° C. and stirred for 15 min. After the LDA solution was cooled to −78° C., compound S1-6 (2.26 g, 5.44 mmol, 1.0 equiv) in THF (5 mL) was added dropwise. An orange-red solution was formed. After 10 min, DMF (1.26 mL, 16.30 mmol, 3.0 equiv) was added dropwise. The reaction solution was allowed to warm to −20° C. in 1 h and quenched by saturated aqueous NH₄Cl. LC/MS indicated that the starting material was consumed. The reaction mixture was diluted with EtOAc (100 mL), washed with water and brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure. This gave 2.42 g of crude S1-7, which was purified by column chromatography (Biotage 24 g column, 5 to 10% EtOAc in hexanes gradient), yielding 2.23 g (92%) of the pure compound S1-7 as a light yellow solid: ¹H NMR (400 MHz, CDCl₃) δ 10.37 (s, 1H), 7.51-7.47 (m, 2H), 7.40-7.33 (m, 5H), 7.27 (t, J=7.3 Hz, 1H), 7.06-7.02 (m, 22H), 5.12 (s, 2H), 2.37 (d, J=2.3 Hz, 3H).

To a solution of compound S1-7 (10 g, 22.60 mmol, 1.0 equiv) in MeOH was added trimethylorthoformate (4.8 g, 45.20 mmol, 2.0 equiv) and TsOH.H₂O (0.13 g, 0.68 mmol, 0.03 equiv) at rt. The reaction mixture was heated to reflux overnight and concentrated under reduced pressure. The residue was diluted with H₂O and extracted with EtOAc. The organic layer was dried over sodium sulfate and evaporated to dryness. The crude product was purified by column chromatography on silica gel (petroleum ether:EtOAc from 100:1 to 30:1) to afford compound S1-8 as a light yellow solid (10 g, 91%): ¹H NMR (400 MHz, CDCl₃) δ 7.41-7.45 (m, 2-1), 7.25-7.35 (m, 5H), 7.16-7.21 (m, 1H), 6.98 (d, J=8.0 Hz, 2H), 5.71 (s, 1H), 5.04 (s, 2H), 3.46 (s, 6H), 2.29 (d, J=2.4 Hz, 3H).

Compound S1-8 (1.37 g, 2.80 mmol, 3.0 equiv) and enone S1-9 (0.45 g, 0.93 mmol, 1.0 equiv) were dissolved in dry THF (5 mL) under N₂. Freshly prepared LDA/TMEDA/THF (0.71 M/THF, 7.9 mL, 5.60 mmol, 2.0 equiv) was added to the solution at −78° C. The solution was stirred at −78° C. for about 10 min, and then the temperature was slowly increased from −78° C. to −10° C. over 20 min. The reaction mixture was quenched by saturated aqueous NH₄Cl (50 mL) and extracted with EtOAc (50 ml×3). The organic phase was dried over sodium sulfate and evaporated under reduced pressure to afford the crude product. The crude product was purified by column chromatography on silica gel (petroleum ether:EtOAc: from 50:1 to 15:1) to give the desired compound S1-10 (0.60 g, 0.68 mmol, 74%): ¹H NMR (400 MHz, CD₃OD) δ 15.97 (s, 1H), 7.61-7.59 (m, 2H), 7.55-7.53 (m, 2H), 7.42-7.40 (m, 6H), 5.83 (s, 1H), 5.40 (s, 2H), 5.02-4.97 (m, 2H), 3.96 (d, J=10.8 Hz, 1H), 3.57 (s, 6H), 3.35-3.26 (m, 1H), 3.09-2.95 (m, 1H), 2.67-2.58 (m, 1H), 2.53 (s, 6H), 2.50-2.39 (m, 1H), 2.21-2.10 (m, 1H), 1.58 (s, 9H), 0.31 (s, 3H), 0.16 (s, 3H); MS (ESI) m/z 877.3 (M+H).

Preparation of LDA/TMEDA/THF: To diisopropylamine (1.1 g, 10.90 mmol, 1.0 equiv) and TMEDA (5 mL) in dry THF (5 mL) at −78° C. was added n-BuLi (4.8 mL, 2.5 M/hexanes, 12.00 mmol, 1.1 equiv) dropwise under N₂. The solution was stirred at −78° C. for 1 h. The prepared LDA/TMEDA/THF solution was about 0.71 M and was used immediately.

Compound S1-10 (50 mg, 0.057 mmol, 1.0 equiv) was dissolved in dry dichloromethane (1 mL). TFA (0.5 mL) was added. The solution was stirred at 10° C. for 1 h. LC-MS analysis showed the complete consumption of starting material. The reaction mixture was washed with H₂O (10 mL×3) and concentrated under reduced pressure to give crude S1-11, which was used for the next step without further purification: MS (ESI) m/z 741.1 (M+H).

Compound S1-11 (crude, 0.057 mmol, 1.0 equiv) was dissolved in 1,2-dichloroethane (2 mL). HOAc (20 μL) and n-propylamine (49 mg, 0.34 mmol, 6.0 equiv) were added. The mixture was stirred for 1 h. Na(OAc)₃BH (73 mg, 0.34 mmol, 6.0 equiv) was added and the resulting mixture was stirred for another hour. The mixture was washed with H₂O (10 mL) and concentrated to give crude S1-12-1, which was used for the next step without further purification: MS (ESI) m/z 786.2 (M+H).

Compound S1-12-1 (crude, 0.057 mmol, 1.0 equiv) was dissolved in 1,2-dichloroethane (2 mL). HOAc (20 μL) and propionaldehyde (49 mg, 0.34 mmol, 6.0 equiv) were added. The mixture was stirred for 1 h. Na(OAc)₃BH (73 mg, 0.34 mmol, 6.0 equiv) was added and the resulting mixture was stirred for another hour. The mixture was washed with H₂O (10 mL) and concentrated to give crude S1-12-2, which was used for the next step without further purification: MS (ESI) m/z 828.2 (M+H).

Compound S1-12-2 (crude, 0.057 mmol, 1.0 equiv) was dissolved in THF (5 mL) in a polypropylene tube at rt. Aqueous HF (2 mL, 48-50%) was added. The reaction mixture was stirred at rt for 1 h. The resulting mixture was carefully poured into an aqueous solution of K₂HPO₄. The pH of the mixture was adjusted to 7-8 by adding more aqueous K₂HPO₄. The mixture was extracted with EtOAc (20 mL), and the EtOAc extract was concentrated to give crude S1-13-2, which was used for the next step without further purification: MS (ESI) m/z 714.0 (M+H).

Compound S1-13-2 (crude, 0.057 mmol, 1.0 equiv) was dissolved in MeOH (5 mL). HCl/MeOH (1 mL, 4 M) and 10% Pd—C (15 mg) was added. The reaction mixture was purged with hydrogen and stirred under H₂ (balloon) at rt for 1 h. The mixture was filtered and concentrated. The crude compound was purified by preparative HPLC using similar conditions for S2-4-1 to afford the desired compound S1-14-2 as a yellow solid (10 mg, 32%, 5 steps): ¹H NMR (400 MHz, CD₃OD) δ 7.06 (d, J=6.0 Hz, 1H), 4.41 (s, 2H), 4.10 (s, 1H), 3.18-2.91 (m, 13H), 2.37-2.23 (m, 2H), 1.83-1.73 (m, 4H), 1.67-1.58 (m, 1H), 0.96 (t, J=7.2 Hz, 3H); MS (ESI) m/z 546.2 (M+H).

Similarly, compound S1-14-1 was prepared directly from S1-12-1 via HF treatment followed by hydrogenation: ¹H NMR (400 MHz, CD₃OD) δ 7.03 (d, J=6.0 Hz, 1H), 4.27 (s, 2H), 4.12 (s, 1H), 3.21 (dd, J=15.1, 4.6 Hz, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 2.94 (d, J=6.9 Hz, 2H), 3.14-2.98 (m, 2H), 2.21-2.39 (m, 2H), 1.82-1.71 (m, 2H), 1.70-1.58 (m, 1H), 1.02 (t, J=7.6 Hz, 3H); MS (ESI) m/z 504.44 (M+H).

Compound S1-11 (crude, 0.057 mmol, 1.0 equiv) was dissolved in 1,2-dichloroethane (2 mL). HOAc (20 μL) and (S)-(+)-2-(methyoxymethyl)pyrrolidine (49 mg, 0.34 mmol, 6.0 equiv) were added. The mixture was stirred at rt for 1 h. Na(OAc)₃BH (73 mg, 0.34 mmol, 6.0 equiv) was added and the resulting mixture was stirred for another hour. The mixture was washed with H₂O (10 mL) and concentrated to give crude S1-12-3, which was used for the next step without further purification: MS (ESI) m/z 842.3 (M+H).

Compound S1-12-3 (crude, 0.057 mmol, 1.0 equiv) was dissolved in THF (5 mL) in a polypropylene tube at rt. Aqueous HF (2 mL, 48-50%) was added. The reaction mixture was stirred at rt for 1 h. The resulting mixture was poured into an aqueous solution of K₂HPO₄. The pH of the mixture was adjusted to 7-8 with more aqueous K₂HPO₄. The mixture was extracted with EtOAc (20 mL), and the EtOAc extract was concentrated to give crude S1-13-3, which was used for the next step without further purification: MS (ESI) m/z 728.2 (M+H).

Compound S1-13-3 (crude, 0.057 mmol, 1.0 equiv) was dissolved in MeOH (5 mL). HCl/MeOH (1 mL, 4 M) and 100% Pd—C (15 mg) was added. The reaction mixture was purged with hydrogen and stirred under H₂ (balloon) at rt for 1 h. The mixture was filtered and concentrated. The crude product was purified by preparative HPLC using similar conditions for S2-4-1 to afford the desired compound S1-14-3 as a yellow solid (10 mg, 31%, 4 steps): ¹H NMR (400 MHz, CD₃OD) δ 7.03 (d, J=5.6 Hz, 1H), 4.68 (d, J=13.2 Hz, 1H), 4.29 (d, J=13.2 Hz, 1H), 4.10 (s, 1H), 3.85-3.82 (m, 1H), 3.71-3.59 (m, 2H), 3.46-3.41 (m, 1H), 3.39 (s, 3H), 3.26-2.93 (m, 10H), 2.33-2.24 (m, 3H), 2.22-2.09 (m, 1H), 1.95-1.81 (m, 2H), 1.63-1.55 (m, 1H); MS (ESI) m/z 560.1 (M+H).

The following compounds were prepared similarly to S1-14-1, S1-14-2, or S1-14-3.

S1-14-4:

¹H NMR (400 MHz, CD₃OD with DCl) δ 7.09 (d, J=5.96 Hz, 1H), 4.23 (s, 2H), 4.16 (s, 1H), 3.26-2.94 (m, 9H), 2.38-2.20 (m, 5H), 2.12-2.00 (m, 6H), 1.84-1.71 (m, 6H), 1.70-1.56 (m, 1H); MS (ESI) m/z 596.18 (M+H).

S1-14-5:

¹H NMR (400 MHz, CD₃OD with DCl) δ 7.09 (d, J=5.5 Hz, 1H), 4.89-4.79 (m, 1H), 4.17 (s, 1H), 3.98-3.89 (m, 1H), 3.26-2.94 (m, 9H), 2.74 (s, 3H), 2.38-2.05 (m, 1H), 1.84-1.71 (m, 6H), 1.70-1.56 (m, 1H); MS (ESI) m/Z 610.19 (M+H).

S1-14-6:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=6.0 Hz, 1H), 4.40 (d, J=14.2 Hz, 1H), 4.34 (d, J=14.2 Hz, 1H), 4.10 (s, 1H), 3.21 (dd, J=15.5, 4.6 Hz, 1H), 3.04 (s, 3H), 2.95 (s, 3H), 3.17-2.97 (m, 2H), 2.34 (t, J=14.7 Hz, 1H), 2.28-2.20 (m, 1H), 1.69-1.59 (m, 1H), 1.34 (d, J=6.9 Hz, 3H), 1.00 (s, 9H); MS (ESI) m/z 546.30 (M+H).

S1-14-7:

¹H NMR (400 MHz, CD₃OD) δ 7.04 (d, J=6.0 Hz, 1H), 4.40 (d, J=14.2 Hz, 1H), 4.29 (d, J=14.2 Hz, 1H), 4.10 (s, 1H), 3.21 (dd, J=15.5, 4.6 Hz, 1H), 3.04 (s, 3H), 2.96 (s, 3H), 3.17-2.97 (m, 2H), 2.73 (m, 1H), 2.34 (t, J=14.7 Hz, 1H), 2.23 (m, 1H), 1.69-1.59 (m, 1H), 1.46 (d, J=6.9 Hz, 3H), 1.00 (s, 9H), 1.07-0.99 (m, 1H), 0.79-0.72 (m, 2H), 0.63-0.55 (m, 1H), 0.41-0.32 (m, 1H); MS (ESI) m/z 530.25 (M+H).

S1-14-8:

¹H NMR (400 MHz, CD₃OD) δ 7.09 (d, J=6.0 Hz, 1H), 4.69 (dd, J=30.2, 13.3 Hz, 1H), 4.20 (dd, J=30.2, 13.3 Hz, 1H), 4.11 (s, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 3.26-2.97 (m, 3H), 2.85 (d, J=21.1 Hz, 3H), 2.36 (t, J=14.6 Hz, 1H), 2.29-2.21 (m, 1H), 1.69-1.60 (m, 1H), 1.52 (t, J=5.0 Hz, 3H), 1.30-1.12 (m, 1H), 0.90-0.74 (m, 2H), 0.72-0.64 (m, 1H), 0.59-0.51 (m, 1H), 0.49-0.37 (m, 1H); MS (ESI) m/z 544.28 (M+H).

S1-14-9:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=6.0 Hz, 1H), 4.73 (dd, J=29.8, 13.3 Hz, 1H), 4.25 (dd, J=30.2, 13.3 Hz, 1H), 4.10 (s, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 3.50-2.97 (m, 5H), 2.36 (t, J=14.6 Hz, 1H), 2.28-2.20 (m, 1H), 1.70-1.60 (m, 1H), 1.48 (dd, J=18.3, 6.4 Hz, 3H), 1.29 (dt, J=30.7, 6.4 Hz, 1H), 0.90-0.73 (m, 2H), 0.65-0.49 (m, 2H), 0.46-0.37 (m, 1H); MS (ESI) m/z 558.29 (M+H).

S1-14-10:

¹H NMR (400 MHz, CD₃OD) δ 7.06 (d, J=6.0 Hz, 1H), 4.58 (d, J=13.2 Hz, 1H), 4.17 (d, J=13.2 Hz, 1H), 4.11 (s, 1H), 3.63-3.55 (m, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 3.25-2.97 (m, 5H), 2.36 (t, J=14.7 Hz, 1H), 2.30-2.22 (m, 1H), 1.71-1.61 (m, 1H), 1.36 (d, J=6.9 Hz, 3H), 1.21 (s, 9H); MS (ESI) m/z 574.32 (M+H).

S1-14-11:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=6.0 Hz, 1H), 4.39 (t, J=13.3 Hz, 2H), 4.13 (s, 1H), 3.93 (dd, J=12.3, 2.7 Hz, 1H), 3.80 (dd, J=12.4, 6.9 Hz, 1H), 3.06 (s, 3H), 2.97 (s, 3H), 3.23-2.97 (m, 4H), 2.37-2.23 (m, 2H), 2.22-2.16 (m, 1H), 2.13 (d, J=1.4 Hz, 1H), 1.68-1.58 (m, 1H), 1.06 (dd, J=23.4, 6.4 Hz, 6H); MS (ESI) m/z 548.25 (M+H).

S1-14-12:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=6.0 Hz, 1H), 4.48 (m, 2H), 4.13 (s, 1H), 3.98 (dd, J=12.4, 2.7 Hz, 1H), 3.85 (dd, J=11.0, 7.8 Hz, 1H), 3.06 (s, 3H), 2.98 (s, 3H), 3.23-2.97 (m, 4H), 2.40-2.23 (m, 2H), 2.10 (d, J=1.4 Hz, 1H), 1.69-1.59 (m, 1H), 1.06 (s, 9H); MS (ESI) m/z 562.26 (M+H).

S1-14-13:

¹H NMR (400 MHz, CD₃OD) δ 7.11 (br, s, 1H), 4.71 (d, J=13.3 Hz, 1H), 4.39 (d, J=13.3 Hz, 1H), 4.13 (s, 1H), 4.04-3.88 (m, 2H), 3.80 (dd, J=12.4, 6.9 Hz, 1H), 3.06 (s, 3H), 2.97 (s, 3H), 3.23-2.92 (m, 7H), 2.47-2.23 (m, 3H), 1.69-1.59 (m, 1H), 1.18-1.02 (m, 6H); MS (ESI) m/z 562.27 (M+H).

S1-14-14:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (d, J=5.5 Hz, 1H), 4.25 (d, J=14.7 Hz, 1H), 4.05 (d, J=14.7 Hz, 1H), 4.08 (s, 1H), 3.81-3.73 (m, 1H), 3.04 (s, 3H), 2.96 (s, 3H), 3.27-2.97 (m, 4H), 2.76 (m, 1H), 2.35-2.17 (m, 3H), 2.09-1.99 (m, 1H), 1.99-1.87 (m, 2H), 1.69-1.59 (m, 1H); MS (ESI) m/z 584.26 (M+H).

S1-14-15:

¹H NMR (400 MHz, CD₃OD) δ 7.06 (d, J=5.9 Hz, 1H), 4.27 (s, 2H), 4.12 (s, 1H), 3.02 (s, 3H), 3.27-2.96 (m, 8H), 2.40-2.24 (m, 2H), 1.87-1.58 (m, 7H), 1.40-1.19 (m, 3H), 1.11-0.98 (m, 2H); MS (ESI) m/z 558.31 (M+H).

S1-14-16:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=5.5 Hz, 1H), 4.45 (s, 2H), 4.10 (s, 1H), 3.27-2.98 (m, 7H), 3.04 (s, 3H), 2.97 (s, 3H), 2.37 (t, J=15.1 Hz, 1H), 2.27-2.17 (m, 1H), 2.14 (s, 3H), 1.70-1.60 (m, 1H); MS (ESI) m/z 573.26 (M+H).

S1-14-17:

¹H NMR (400 MHz, CD₃OD) δ 7.11 (d, J=5.5 Hz, 1H), 4.47 (s, 2H), 4.09 (s, 1H), 3.27-2.98 (m, 7H), 3.04 (s, 3H), 2.97 (s, 3H), 2.96 (s, 3H), 2.36 (t, J=15.1 Hz, 1H), 2.26-2.18 (m, 1H), 1.70-1.60 (m, 1H); MS (ESI) m/z 609.20 (M+H).

S1-14-18:

¹H NMR (400 MHz, CD₃OD) δ 7.09 (d, J=6.0 Hz, 1H), 4.77 (d, J=13.3 Hz, 1H), 4.55 (d, J=13.3 Hz, 1H), 4.12-4.06 (m, 3H), 3.14 (s, 3H), 3.04 (s, 3H), 2.96 (s, 3H), 3.23-2.97 (m, 4H), 2.42-3.34 (m, 1H), 2.23 (m, 1H), 1.70-1.60 (m, 1H), 1.02 (s, 9H); MS (ESI) m/z 576.27 (M+H).

S1-14-19:

¹H NMR (400 MHz, CD₃OD) δ 7.09 (d, J=5.5 Hz, 1H), 4.53 (s, 2H), 4.08 (s, 1H), 3.90 (s, 2H), 3.67-3.55 (m, 4H), 3.27-2.98 (m, 3H), 3.04 (s, 3H), 2.96 (s, 3H), 2.38 (t, J=15.1 Hz, 1H), 2.27-2.19 (m, 1H), 1.70-1.60 (m, 1H); MS (ESI) m/z 545.19 (M+H).

S1-14-20:

¹H NMR (400 MHz, CD₃OD) δ 7.03 (d, J=5.9 Hz, 1H), 4.51 (d, J=4.1 Hz, 2H), 4.07 (s, 1H), 3.03 (s, 3H), 2.95 (s, 3H), 3.25-2.96 (m, 3H), 2.35 (t, J=15.1 Hz, 1H), 2.26-2.18 (m, 1H), 1.70-1.60 (m, 1H), 1.59-1.51 (m, 1H), 1.02-0.94 (m, 2H), 0.86-0.78 (m, 2H), 0.76-0.68 (m, 2H), 0.43-0.35 (m, 2H); MS (ESI) m/z 542.29 (M+H).

S1-14-21:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=5.5 Hz, 1H), 4.41 (s, 2H), 4.08 (s, 1H), 3.72-3.64 (m, 2H), 3.24-2.98 (m, 5H), 3.04 (s, 3H), 2.96 (s, 3H), 2.65-2.55 (m, 1H), 2.37 (t, J=15.1 Hz, 1H), 2.24-2.10 (m, 3H), 1.90-1.77 (m, 2H), 1.71-1.61 (m, 1H); MS (ESI) m/z 598.30 (M+H).

S1-14-22:

¹H NMR (400 MHz, CD₃OD) δ 7.01 (d, J=6.0 Hz, 1H), 4.32 (s, 2H), 4.09 (s, 1H), 3.87 (s, 2H), 3.24-2.98 (m, 3H), 3.04 (s, 3H), 2.96 (s, 3H), 2.35 (t, J=15.1 Hz, 1H), 1.98, (s, 1.5H), 1.70-1.60 (m, 1H); MS (ESI) m/z 519.23 (M+H).

S1-14-23:

¹H NMR (400 MHz, CD₃OD) δ 7.06 (d, J=5.5 Hz, 1H), 4.34 (s, 2H), 4.08 (s, 1H), 3.58-3.50 (m, 2H), 3.24-2.98 (m, 5H), 3.03 (s, 3H), 2.96 (s, 3H), 2.36 (t, J=15.1 Hz, 1H), 2.23 (m, 1H), 2.02-1.92 (m, 2H), 1.69-1.59 (m, 1H), 1.54-1.40 (m, 3H), 1.40-1.32 (m, 1H), 0.91 (d, J=6.4 Hz, 6H); MS (ESI) m/z 572.27 (M+H).

S1-14-24:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=6.0 Hz, 1H), 4.51-2.43 (m, 2H), 4.08 (s, 1H), 4.04 (s, 2H), 3.24-2.98 (m, 3H), 3.03 (s, 3H), 2.96 (s, 3H), 2.92 (s, 3H), 2.35 (t, J=15.1 Hz, 1H), 2.28-2.18 (m, 1H), 1.69-1.59 (m, 1H); MS (ESI) m/z 533.29 (M+H).

S1-14-25:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=5.5 Hz, 1H), 4.31 (s, 2H), 4.10 (s, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 3.27-2.96 (m, 3H), 2.77 (s, 2H), 2.61 (s, 1H), 2.36 (t, J=15.1 Hz, 1H), 2.28-2.20 (m, 1H), 1.84-1.76 (m, 4H), 1.75-1.66 (m, 5H), 1.63 (br, s, 5H), 1.59 (br, s, 2H); MS (ESI) m/z 610.28 (M+H).

S1-14-26:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (br, s, 1H), 4.37 (s, 2H), 4.07 (s, 1H), 3.92-2.84 (m, 1H), 3.61-3.54 (m, 2H), 3.24-2.98 (m, 5H), 3.03 (s, 3H), 2.96 (s, 3H), 2.36 (t, J=15.1 Hz, 1H), 2.24-2.10 (m, 2H), 2.18-1.96 (m, 1H), 1.91 (s, 3H), 1.80-1.59 (m, 3H); MS (ESI) m/z 587.24 (M+H).

S1-14-27:

¹H NMR (400 MHz, CD₃OD) δ 7.03 (d, J=5.5 Hz, 1H), 4.24 (s, 2H), 4.09 (s, 1H), 3.04 (s, 3H), 2.96 (s, 3H), 3.27-2.96 (m, 4H), 2.57 (d, J=4.2 Hz, 1H), 2.43 (s, 1H), 2.34 (t, J=15.1 Hz, 1H), 2.27-2.19 (m, 1H), 1.87 (dd, J=13.7, 7.8 Hz, 1H), 1.73-1.52 (m, 5H), 1.35 (d, J=11.0 Hz, 1H), 1.28-1.20 (m, 2H); MS (ESI) m/z 556.30 (M+H).

S1-14-28:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=5.9 Hz, 1H), 4.31 (s, 2H), 4.09 (s, 1H), 3.47 (s, 1H), 3.04 (s, 3H), 2.96 (s, 3H), 3.27-2.96 (m, 3H), 2.57 (d, J=4.2 Hz, 1H), 2.43 (s, 1H), 2.35 (t, J=15.5 Hz, 1H), 2.26 (s, 2H), 2.29-2.20 (m, 1H), 2.04-1.89 (m, 6H), 1.87-1.73 (m, 6H), 1.70-1.60 (m, 1H); MS (ESI) m/z 596.36 (M+H).

S1-14-29:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=5.9 Hz, 1H), 4.39 (s, 2H), 4.10 (s, 1H), 3.65-3.56 (m, 2H), 3.24-2.98 (m, 5H), 3.04 (s, 3H), 2.97 (s, 3H), 2.60-2.52 (m, 1H), 2.37 (t, J=15.1 Hz, 1H), 2.28-2.20 (m, 1H), 2.13-2.02 (m, 2H), 1.97-1.88 (m, 2H), 1.70-1.60 (m, 1H); MS (ESI) m/z 573.28 (M+H).

S1-14-30:

¹H NMR (400 MHz, CD₃OD) δ 7.25-7.19 (m, 5H), 7.02 (d, J=5.6 Hz, 1H), 4.42 (s, 2H), 3.98 (s, 1H), 3.34-3.21 (m, 4H), 3.15-2.85 (m, 11H), 2.30-2.18 (m, 2H), 1.62-1.52 (m, 1H), 1.33 (t, J=7.2 Hz, 3H); MS (ESI) m/z 594.1 (M+H).

S1-14-31:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (d, J=5.6 Hz, 1H), 4.88 (t, J=4.4 Hz, 1H), 4.55 (t, J=4.4 Hz, 1H), 4.01 (s, 1H), 3.70-3.50 (m, 2H), 3.17 (d, J=7.2 Hz, 2H), 3.10-2.87 (m, 11H), 2.31-2.12 (m, 2H), 1.57-1.54 (m, 1H), 1.13-1.08 (m, 1H), 0.74-0.72 (m, 2H), 0.41-0.37 (m, 2H); MS (ESI) m/z 562.1 (M+H).

S1-14-32:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (d, J=5.6 Hz, 1H), 4.87 (t, J=4.0 Hz, 1H), 4.74 (t, J=4.0 Hz, 1H), 4.43 (s, 2H), 4.02 (s, 1H), 3.60-3.51 (m, 2H), 3.20-2.88 (m, 11H), 2.31-2.15 (m, 2H), 1.78-1.7 (m, 2H), 1.58-1.55 (m, 1H), 0.93 (m, J=7.2 Hz, 3H); MS (ESI) m/z 550.1 (M+H).

S1-14-33:

¹H NMR (400 MHz, CD₃OD) δ 7.01 (d, J=5.6 Hz, 1H), 4.56 (d, J=13.2 Hz, 1H), 4.13 (d, J=13.2 Hz, 1H), 4.01 (s, 1H), 3.56-3.36 (m, 2H), 3.16-2.86 (m, 10H), 2.33-2.16 (m, 3H), 2.07-1.91 (m, 2H), 1.73-1.67 (m, L H), 1.61-1.52 (m, 1H), 1.42 (d, J=6.4 Hz, 3H); MS (ESI) m/z 575.2 (M+H).

S1-14-34:

¹H NMR (400 MHz, CD₃OD) δ 7.01 (d, J=5.6 Hz, 1H), 4.56 (d, J=13.2 Hz, 1H), 4.13 (d, J=13.2 Hz, 1H), 4.01 (s, 1H), 3.56-3.36 (m, 2H), 3.16-2.86 (m, 10H), 2.33-2.16 (m, 3H), 2.07-1.91 (m, 2H), 1.73-1.67 (m, 1H), 1.61-1.52 (m, 1H), 1.42 (d, J=6.4 Hz, 3H); MS (ESI) m/z 575.2 (M+H).

S1-14-35:

¹H NMR (400 MHz, CD₃OD) δ 7.09 (d, J=6.0 Hz, 1H), 4.96 (t, J=6.0 Hz, 1H), 4.78 (t, J=6.0 Hz, 1H), 4.51 (s, 2H), 4.11 (s, 1H), 3.72-3.59 (m, 2H), 3.40 (q, J=6.8 Hz, 2H), 3.24-2.97 (m, 9H), 2.39-2.24 (m, 2H), 1.69-1.60 (m, 1H), 1.41 (t, J=7.2 Hz, 3H); MS (ESI) m/z 536.1 (M+H).

S1-14-36:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=6.0 Hz, 1H), 4.62-4.47 (m, 2H), 4.12 (s, 1H), 3.50-3.44 (m, 2H), 3.23-2.98 (m, 9H), 2.38-2.25 (m, 2H), 1.69-1.66 (m, 1H), 1.55 (s, 3H), 1.42-1.39 (t, J=7.2 Hz, 3H), 1.39-1.35 (m, 3H), 0.94-0.86 (m, 3H); MS (ESI) m/z 544.2 (M+H).

S1-14-37:

¹H NMR (400 MHz, CD₃OD) δ 7.09 (d, J=6.0 Hz, 1H), 4.59 (s, 2H), 4.10 (s, 1H), 3.24-2.97 (m, 11H), 2.40-2.24 (m, 2H), 1.70-1.59 (m, 1H), 1.56 (s, 3H), 1.21-1.15 (m, 1H), 0.93-0.89 (m, 2H), 0.79-0.77 (m, 2H), 0.69-0.66 (m, 1H), 0.59-0.56 (m, 1H), 0.47-0.46 (m, 2H); MS (ESI) m/z 570.1 (M+H).

S1-14-38:

¹H NMR (400 MHz, CD₃OD) δ 7.09 (d, J=6.0 Hz, 1H), 4.63-4.60 (m, 1H), 4.50-4.47 (m, 1H), 4.11 (s, 1H), 3.13-2.98 (m, 1H), 2.39-2.24 (m, 2H), 1.94-1.92 (m, 1H), 1.75-1.70 (m, 1H); 1.66-1.63 (m, 1H), 1.58 (s, 3H), 1.38-1.29 (m, 1H), 1.02 (t, J=7.2 Hz, 3H), 0.92-0.87 (m, 3H); MS (ESI) m/z 558.1 (M+H).

S1-14-39:

¹H NMR (400 MHz, CD₃OD) δ 7.34 (s, 4H), 7.04 (d, J=5.2 Hz, 1H), 4.71-4.63 (m, 4H), 4.63 (s, 2H) 3.99 (s, 1H), 3.17-2.87 (m, 9H), 2.28-2.15 (m, 2H) 1.61-1.51 (m, 1H); MS (ESI) m/z 564.2 (M+H).

S1-14-40:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=5.6 Hz, 1H), 4.42 (d, J=4.0 Hz, 2H), 4.12 (s, 1H), 3.29-2.90 (m, 13H), 2.41-2.22 (m, 2H), 1.90-1.75 (m, 2H), 1.71-1.60 (M, 1H); 1.38 (t, J=7.2 Hz, 3H), 1.05 (t, J=7.2 Hz, 3H); MS (ESI) m/z 530.2 (M+H).

S1-14-41:

¹H NMR (400 MHz, CD₃OD) δ 7.05 (d, J=5.2 Hz, 1H), 4.48 (d, J=13.2 Hz, 1H), 4.27 (d, J=13.2 Hz, 1H), 4.09 (d, J=4.4 Hz, 1H), 3.22-2.92 (m, 11H), 2.84 (s, 3H), 2.38-2.22 (m, 2H), 1.87-1.70 (m, 2H), 1.68-1.62 (m, 1H), 1.03 (t, J=7.2 Hz, 3H); MS (ESI) m/z 518.0 (M+H).

S1-14-42:

¹H NMR (400 MHz, CD₃OD) δ 7.05 (d, J=5.6 Hz, 1H), 4.51 (d, J=13.6 Hz, 1H), 4.28 (d, J=13.6 Hz, 1H), 4.09 (s, 1H), 3.20-2.95 (m, 11H), 2.83 (s, 3H), 2.40-2.13 (m, 2H), 1.79-1.71 (m, 2H), 1.69-1.57 (m, 1H), 1.46-1.35 (m, 2H), 0.98 (t, J=7.2 Hz, 3H); MS (ESI) m/z 532.1 (M+H).

S1-14-43:

¹H NMR (400 MHz, CD₃OD) δ 7.27-7.16 (m, 5H), 7.01 (d, J=5.6 Hz, 1H), 4.52-4.48 (m, 1H), 4.29-4.25 (m, 1H), 4.03 (s, 1H), 3.45-3.25 (m, 2H), 3.15-2.88 (m, 11H), 2.84 (s, 3H), 2.30-2.16 (m, 2H), 1.60-1.54 (m, 1H); MS (ESI) m/z 580.1 (M+H).

S1-14-44:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=4.8 Hz, 1H), 4.40 (s, 2H), 4.11 (s, 1H), 3.29-2.95 (m, 13H), 2.37-2.23 (m, 2H), 1.79-1.71 (m, 2H), 1.67-1.58 (m, 1H), 1.45-1.38 (m, 2H), 1.37 (t, J=7.2 Hz, 3H), 0.95 (t, J=7.2 Hz, 3H); MS (ESI) m/z 546.2 (M+H).

S1-14-45:

¹H NMR (400 MHz, CD₃OD) δ 7.03 (d, J=5.6 Hz, 1H), 4.70 (d, J=13.2 Hz, 1H), 4.29 (d, J=13.2 Hz, 1H), 4.09 (s, 1H), 3.86-3.83 (m, 1H), 3.73-3.60 (m, 2H), 3.49-3.41 (m, 1H), 3.40 (s, 3H), 3.22-2.95 (m, 10H), 2.37-2.26 (m, 3H), 2.22-1.84 (m, 3H), 1.68-1.57 (m, 1H); MS (ESI) m/z 560.1 (M+H).

S1-14-46:

¹H NMR (400 MHz, CD₃OD) δ 7.47-7.41 (m, 5H), 6.95 (d, J=6.0 Hz, 1H), 4.41-4.36 (m, 2H), 4.34-4.18 (m, 2H), 4.02 (s, 1H), 3.12-2.88 (m, 9H), 2.72 (s, 3H), 2.28-2.12 (m, 2H), 1.60-1.50 (m, 1H); MS (ESI) m/z 565.2 (M+H).

S1-14-47:

¹H NMR (400 MHz, CD₃OD) δ 7.58-7.49 (m, 5H), 6.99 (d, J=5.6 Hz, 1H), 4.49-4.42 (m, 4H), 4.13 (s, 1H), 3.18-2.97 (m, 11H), 2.35-2.23 (m, 2H), 1.91-1.88 (m, 2H), 1.68-1.47 (m, 1H), 0.97 (t, J=7.2 Hz, 3H); MS (ESI) m/z 594.2 (M+H).

S1-14-48:

¹H NMR (400 MHz, CD₃OD) δ 7.53-7.49 (m, 5H), 6.96 (d, J=5.2 Hz, 1H), 4.62-4.35 (m, 4H), 4.09 (s, 1H), 3.20-2.96 (m, 11H), 2.35-2.21 (m, 2H), 1.67-1.48 (m, 1H), 1.26-1.13 (m, 1H), 0.86-0.74 (m, 2H), 0.44-0.33 (m, 2H); MS (ESI) m/z 606.1 (M+H).

S1-14-49:

¹H NMR (400 MHz, CD₃OD) δ 7.53-7.48 (m, 5H), 6.96 (d, J=5.6 Hz, 1H), 4.48-4.34 (m, 4H), 4.09 (s, 1H), 3.26-2.87 (m, 11H), 2.35-2.20 (m, 2H), 1.62-1.56 (m, 1H), 1.44 (t, J=7.2 Hz, 3H); MS (ESI) m/z 580.1 (M+H).

S1-14-50:

¹H NMR (400 MHz, CD₃OD) δ 7.11 (d, J=5.6 Hz, 1H), 5.47 (d, J=52 Hz, 1H), 4.55 (s, 2H), 4.12 (s, 1H), 3.95-3.47 (m, 4H), 3.24-2.98 (m, 9H), 2.70-2.62 (m, 1H), 2.39-2.25 (m, 3H), 1.69-1.61 (m, 1H); MS (ESI) m/z 534.1 (M+H).

S1-14-51:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=6 Hz, 1H), 5.47 (d, J=52.4 Hz, 1H), 4.54 (s, 2H), 4.11 (s, 1H), 3.92-3.39 (m, 4H), 3.26-2.98 (m, 9H), 2.70-2.62 (m, 1H), 2.40-2.23 (m, 3H), 1.71-1.62 (m, 1H); MS (ESI) m/z 534.1 (M+H).

S1-14-52:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (d, J=5.6 Hz, 1H), 4.07 (s, 1H), 3.93 (s, 2H), 3.31 (q, J=9.2 Hz, 2H), 3.19-2.89 (m, 9H), 2.77 (q, J=7.2 Hz, 2H), 2.34-2.15 (m, 2H), 1.68-1.57 (m, 1H), 1.11 (t, J=7.2 Hz, 3H); MS (ESI) m/z 572.1 (M+H).

S1-14-53:

¹H NMR (400 MHz, CD₃OD) δ 7.04 (d, J=6.0 Hz, 1H), 4.20 (s, 2H), 4.09 (s, 1H), 3.76-3.65 (m, 2H), 3.74 (q, J=7.2 Hz, 2H), 3.20-2.96 (m, 9H), 2.81 (d, J=6.8 Hz, 2H), 2.34-2.21 (m, 2H), 1.68-1.56 (m, 1H), 1.07-0.98 (m, 1H), 0.65-0.58 (m, 2H), 0.27-0.23 (m, 2H); MS (ESI) m/z 598.1 (M+H).

S1-14-54:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (d, J=6.0 Hz, 1H), 4.45 (s, 2H), 4.25-4.17 (m, 4H), 4.09 (s, 1H), 3.21-2.95 (m, 9H), 2.61-2.51 (m, 1H), 2.49-2.38 (s, 1H), 2.32-2.18 (m, 2H), 1.65-1.53 (m, 1H); MS (ESI) m/z 502.1 (M+H).

S1-14-55:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (d, J=6.0 Hz, 1H), 4.50-4.42 (m, 2H), 4.29-4.21 (m, 2H), 4.09 (s, 1H), 3.92-3.85 (m, 2H), 3.22-2.97 (m, 9H), 2.28-2.33 (m, 2H), 1.66 (ddd, J=11.6, 11.2, 11.6 Hz, 1H), 1.33 (d, J=6.8 Hz, 1H), 1.28 (d, J=6.8 Hz, 2H); MS (ESI) m/z 516.1 (M+H).

S1-14-56:

¹H NMR (400 MHz, CD₃OD) δ 7.05 (d, J=5.2 Hz, 1H), 4.24 (s, 2H), 4.10 (s, 1H), 3.24-2.97 (m, 9H), 2.28-2.23 (m, 2H), 1.98-1.56 (m, 10H), 1.55 (s, 3H); MS (ESI) m/z 558.1 (M+H).

S1-14-57:

¹H NMR (400 MHz, CD₃OD) δ 7.36-7.32 (m, 2H), 7.29-7.27 (m, 3H), 7.12 (d, J=5.6 Hz, 1H), 4.53 (s, 2H), 4.11 (s, 1H), 3.40 (t, 2H), 3.28-2.94 (m, 13H), 2.43-2.22 (m, 2H), 1.92-1.61 (m, 3H), 1.02 (t, J=7.2 Hz, 3H); MS (ESI) m/z 607.3 (M+H).

S1-14-58:

¹H NMR (400 MHz, CD₃OD) δ 7.26-7.19 (m, 5H), 7.05 (d, J=5.6 Hz, 1H), 4.61-4.42 (m, 2H), 4.03 (s, 1H), 3.47-3.33 (m, 2H), 3.17 (d, J==7.2 Hz, 1H), 3.11-2.88 (m, 11H), 2.32-2.14 (m, 2H), 1.61-1.51 (m, 1H), 1.21-1.11 (m, 1H), 0.74-0.72 (m, 2H), 0.41-0.38 (m, 2H); MS (ESI) m/z 620.3 (M+H).

S1-14-59:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (d, J=5.6 Hz, 1H), 4.48 (s, 2H), 4.01 (s, 1H), 3.87 (t, J=11.6 Hz, 2H), 3.87 (t, J=7.6 Hz, 2H), 3.18-2.88 (m, 9H), 2.18-2.07 (m, 2H), 2.33-2.11 (m, 2H), 1.56-1.51 (m, 1H); MS (ESI) m/z 552.2 (M+H).

S1-14-60:

¹H NMR (400 MHz, CD₃OD) δ 7.24-7.11 (m, 5H), 7.07 (d, J=4.8 Hz, 1H), 4.35 (s, 2H), 4.04 (s, 1H), 3.60-3.57 (m, 3H), 3.16-2.80 (m, 11H), 2.31-2.17 (m, 2H), 2.06-1.96 (s, 4H), 1.63-1.52 (m, 1H); MS (ESI) m/z 606.2 (M+H).

S1-14-61:

¹H NMR (400 MHz, CD₃OD) δ 7.04 (d, J=5.6 Hz, 1H), 4.35 (s, 2H), 4.03 (s, 1H), 3.45-3.40 (m, 2H), 3.23-2.90 (m, 11H), 2.32-2.16 (m, 2H), 2.07-1.81 (m, 4H), 1.79-1.65 (m, 4H), 1.63-1.53 (m, 1H); MS (ESI) m/z 544.2 (M+H).

S1-14-62:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (d, J=6.0 Hz, 1H), 4.37 (s, 2H), 4.03 (s, 1H), 3.72-3.65 (m, 2H), 3.19-2.84 (m, 11H), 2.82-2.65 (m, 2H), 2.32-2.16 (m, 2H), 1.70-1.41 (m, 7H); MS (ESI) m/z 556.2 (M+H).

S1-14-63:

¹H NMR (400 MHz, CD₃OD) δ 7.59-7.34 (m, 5H), 6.94 (d, J=5.6 Hz, 1H), 4.63 (s, 2H), 4.10 (s, 1H), 3.19-2.97 (m, 9H), 2.34-2.23 (m, 2H), 1.68 (ddd, J=13.2, 13.2, 13.2 Hz 1H) MS (ESI) m/z 538.2 (M+H).

S1-14-64:

¹H NMR (400 MHz, CD₃OD) δ 7.97 (s, 1H), 7.91 (t, J=2.8 Hz, 1H), 7.66-7.65 (m, 2H), 6.74 (d, J=5.6 Hz, 1H), 4.46 (s, 2H), 4.00 (s, 1H), 3.25-2.88 (m, 9H), 2.27-2.12 (m, 2H), 1.56 (ddd, J=13.6, 13.6, 13.6 Hz, 1H); MS (ESI) m/z 539.2 (M+H).

S1-14-65:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=5.6 Hz, 1H), 4.62 (d, J=13.2 Hz, 1H), 4.19 (d, J=13.2 Hz, 1H), 4.17 (s, 1H), 3.41-2.35 (m, 1H), 3.25-2.99 (m, 9H), 2.71 (s, 3H), 2.41-2.27 (m, 2H), 2.24-2.17 (m, 2H); 2.05-1.92 (m, 2H), 1.78-1.558 (m, 4H), 1.50-1.37 (M, 2H), 1.35-1.20 (m, 1H); MS (ESI) m/z 558.2 (M+H).

S1-14-66:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=6.0 Hz, 1H), 4.57 (d, J=13.6 Hz, 1H), 4.21 (d, J=13.6 Hz, 1H), 4.10 (s, 1H), 3.44-3.35 (m, 1H), 3.30-2.95 (m, 11H), 2.38-2.20 (m, 2H), 2.15-2.05 (m, 2H), 2.00-1.90 (m, 2H), 1.75-1.55 (m, 4H), 1.50-1.35 (m, 2H), 1.35-1.20 (m, 1H), 1.32 (t, J=7.2 Hz, 3H); MS (ESI) m/z 572.2 (M+H).

S1-14-67:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=5.6 Hz, 1H), 4.56 (d, J=13.6 Hz, 1H), 4.25 (d, J=13.6 Hz, 1H), 4.11 (s, 1H), 3.40-3.32 (m, 1H), 3.25-2.95 (m, 11H), 2.40-2.21 (m, 2H), 2.17-2.06 (m, 2H), 1.99-1.90 (m, 2H), 1.85-1.56 (m, 6H), 1.47-1.20 (m, 3H), 0.96 (t, J=7.2 Hz, 3H); MS (ESI) m/z 586.2 (M+H).

S1-14-68:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=5.9 Hz, 1H), 4.56 (d, J=12.0 Hz, 1H), 4.32 (d, J==12.0 Hz, 1H), 4.11 (s, 1H), 3.56-3.48 (m, 1H), 3.21-2.93 (m, 11H), 2.39-2.21 (m, 2H), 2.18-2.04 (m, 2H), 2.0-1.9 (m, 2H), 1.81-1.56 (m, 4H), 1.46-1.20 (m, 3H), 1.15-1.03 (s, 1H), 0.78-0.69 (m, 2H), 0.45-0.35 (m, 2H); MS (ESI) m/z 598.2 (M+H).

S1-14-69:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=5.6 Hz, 1H), 4.30 (s, 2H), 4.11 (s, 1H), 3.28-2.95 (m, 13H), 2.90-2.79 (m, 1H), 2.40-2.15 (m, 4H), 2.07-1.81 (m, 4H), 1.57-1.29 (m, 1H), 1.39-1.33 (t, J=7.2 Hz, 3H); MS (ESI) m/z 557.2 (M+H).

S1-14-70:

¹H NMR (400 MHz, CD₃OD) δ 7.05 (d, J=5.6 Hz, 1H), 4.36 (s, 2H), 4.10 (s, 1H), 3.28-2.96 (m, 13H), 2.90-2.80 (m, 1H), 2.40-2.14 (m, 4H), 2.06-1.72 (m, 6H), 1.67-1.55 (m, 1H), 1.02-0.96 (t, J=7.2 Hz, 3H); MS (ESI) m/z 572.2 (M+H).

S1-14-71:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=5.6 Hz, 1H), 4.55-4.36 (m, 2H), 4.12 (s, 1H), 3.43-2.80 (m, 13H), 2.39-2.12 (m, 4H), 2.08-1.80 (m, 5H), 1.70-1.56 (m, 1H), 1.21-1.12 (m, 1H), 0.82-0.72 (m, 2H), 0.49-0.39 (m, 2H); MS (ESI) m/z 584.2 (M+H).

S1-14-72:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (dd, J=2.0, 6.0 Hz, 1H), 4.55-4.45 (m, 3H), 4.45-3.38 (m, 2H), 4.18-4.02 (m, 3H), 3.33 (s, 3H), 3.22-2.95 (m, 9H), 2.37-2.22 (m, 2H), 1.68-1.58 (m, 1H); MS (ESI) m/z 532.1 (M+H).

S1-14-73:

¹H NMR (400 MHz, CD₃OD) δ 7.14 (d, J=5.6 Hz, 1H), 4.60-4.51 (m, 1H), 4.52-4.45 (m, 1H), 4.11 (s, 1H), 3.25-2.92 (m, 11H), 2.41-2.20 (m, 2H), 2.08-1.75 (m, 8H), 1.68-1.59 (m, 1H), 1.50 (s, 3H), 1.14 (t, J=7.2 Hz, 3H); MS (ESI) m/z 572.2 (M+H).

S1-14-74:

¹H NMR (400 MHz, CD₃OD) δ 7.02 (d, J=5.6 Hz, 1H), 4.25 (s, 2H), 4.11 (s, 1H), 3.22-2.98 (m, 11H), 2.36-2.23 (m, 2H), 1.68-1.58 (m, 1H), 1.33 (t, J=7.2 Hz, 3H); MS (ESI) m/z 490.4 (M+H).

S1-14-75:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=5.6 Hz, 1H), 4.23 (s, 2H), 4.11 (s, 1H), 3.25-2.91 (m, 9H), 2.39-2.20 (m, 2H), 2.00-1.75 (m, 8H), 1.68-1.59 (m, 1H), 1.49 (s, 3H); MS (ESI) m/z 544.3 (M+H).

S1-14-76:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=6.0 Hz, 1H), 4.64-4.47 (m, 2H), 4.11 (s, 1H), 3.25-2.98 (m, 11H), 2.39-2.24 (m, 2H), 1.98-1.90 (m, 1H), 1.80-1.70 (m, 1H), 1.68-1.60 (m, 1H), 1.55 (s, 3H), 1.38-1.29 (m, 1H), 1.02 (t, J=7.2 Hz, 3H), 0.98-0.82 (m, 3H); MS (ESI) m/z 558.1 (M+H).

S1-14-77:

¹H NMR (400 MHz, CD₃OD) δ 7.06 (d, J=5.6 Hz, 1H), 4.30 (s, 2H), 4.12 (s, 1H), 3.24-2.98 (m, 11H), 2.37-2.25 (m, 2H), 1.69-1.60 (m, 1H), 1.19-1.14 (m, 1H), 0.79-0.73 (m, 2H), 0.48-0.42 (m, 2H); MS (ESI) m/z 516.0 (M+H).

S1-14-78:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (d, J=5.6 Hz, 1H), 4.07 (s, 1H), 3.96 (s, 2H), 3.38 (t, J=9.6 Hz, 2), 3.20-2.94 (m, 9H), 2.68 (t, J=7.6 Hz, 2H) 2.35-2.25 (m, 2H), 1.58-1.50 (m, 3H), 0.88 (t, J=7.2 MHz, 3H); MS (ESI) m/z 586.1 (M+H).

S1-14-79:

¹H NMR (400 MHz, CD₃OD) δ 6.94 (d, J=6.0 Hz, 1H), 4.18 (s, 2H), 4.02 (s, 1H), 3.15-2.82 (m, 11H), 2.28-2.15 (m, 2H), 1.66-1.51 (m, 3H), 1.40-1.30 (m, 2H), 0.90 (t, J=7.2 Hz, 3H); MS (ESI) m/z 518.1 (M+H).

S1-14-80:

¹H NMR (400 MHz, CD₃OD) δ 6.91 (d, J=5.6 Hz, 1H), 4.12 (s, 2H), 4.00 (s, 1H), 3.10-2.84 (m, 11H), 2.62-2.54 (m, 1H), 2.24-2.05 (m, 4H), 1.89-1.71 (m, 4H), 1.56-1.47 (m, 1H); MS (ESI) m/z 530.1 (M+H).

S1-14-81:

¹H NMR (400 MHz, CD₃OD) δ 6.92 (d, J=5.2 Hz, 1H), 4.16 (s, 2H), 4.00 (s, 1H), 3.10-2.84 (m, 10H), 2.23-2.06 (m, 4H), 1.79-1.76 (m, 2H), 1.61-1.50 (m, 2H), 1.32-1.10 (m, 5H); MS (ESI) m/z 530.1 (M+H).

S1-14-82:

¹H NMR (400 MHz, CD₃OD) δ 7.01 (d, J=6.0 Hz, 1H), 4.36 (s, 2H), 4.10 (s, 1H), 3.22-2.95 (m, 9H), 2.32-2.23 (m, 2H), 1.67-1.59 (m, 1H), 1.56 (s, 3H), 1.12-1.09 (m, 2H), 0.86-0.83 (m, 2H); MS (ESI) m/z 516.0 (M+H).

S1-14-83:

¹H NMR (400 MHz, CD₃OD) δ 7.04 (d, J=5.2 Hz, 1H), 4.86 (d, J=4.4 Hz, 1H), 4.72 (d, J=4.4 Hz, 1H), 4.35 (s, 2H), 4.11 (s, 1H), 3.52 (d, J=4.0 Hz, 1H), 3.45 (d, J=40 Hz, 1H), 3.23-2.95 (m, 9H), 2.36-2.24 (m, 2H), 1.68-1.58 (m, 1H); MS (ESI) m/z 508.0 (M+H).

S1-14-84:

¹H NMR (400 MHz, CD₃OD) δ 7.15 (d, J=5.6 Hz, 1H), 4.61-4.55 (m, 2H), 4.30-4.19 (m, 1H), 4.11 (s, 1H), 4.05-3.50 (m, 4H), 3.23-2.90 (m, 15H), 2.70-2.60 (m, 1H), 2.55-2.45 (m, 1H), 2.35-2.20 (m, 2H), 1.68-1.57 (m, 1H); MS (ESI) m/z 559.1 (M+H).

S1-14-85:

¹H NMR (400 MHz, CD₃OD) δ 7.13 (d, J=6.06 Hz, 1H), 4.55 (s, 2H), 4.25-4.18 (m, 1H), 4.09 (s, 1H), 3.95-3.48 (m, 4H), 3.23-2.95 (m, 15H), 2.65-2.58 (m, 1H), 2.45-2.39 (m, 1H), 2.38-2.19 (m, 2H), 1.70-1.58 (m, 1H); MS (ESI) m/z 559.1 (M+H).

S1-14-86:

¹H NMR (400 MHz, CD₃OD) δ 7.28-7.16 (m, 5H), 6.93 (d, J=6.0 Hz, 1H), 4.23 (s, 2H), 4.00 (s, 1H), 3.25 (t, J=7.6 Hz, 2H), 3.21-2.88 (m, 11H), 2.28-2.14 (m, 2H), 1.61-1.50 (m, 1H); MS (ESI) m/z 566.1 (M+H).

S1-14-87:

¹H NMR (400 MHz, CD₃OD) δ 7.38-7.27 (m, 5H), 6.90 (d, J=6.0 Hz, 1H), 4.20 (s, 2H), 4.10 (s, 1H), 3.20-2.98 (m, 11H), 2.29-2.20 (m, 2H), 1.69-1.60 (m, 1H), 1.44 (s, 6H); MS (ESI) m/z 594.0 (M+H).

S1-14-88:

¹H NMR (4.00 MHz, CD₃D) δ 7.29-7.23 (m, 4H), 7.11 (d, J=6.0 Hz, 1H), 4.49-4.43 (m, 3H), 4.10 (s, 1H), 3.57-3.48 (m, 4H), 3.24-2.98 (m, 11H), 2.40-2.25 (m, 2H), 1.66-1.58 (m, 1H), 1.41 (t, J=7.2 Hz, 3H); MS (ESI) m/z 605.9 (M+H).

S1-14-89:

¹H NMR (400 MHz, CD₃OD) δ 7.22-7.16 (m, 4H), 7.05 (d, J=6.0 Hz, 1H), 4.48-4.41 (m, 3H), 4.05 (s, 1H), 3.60-3.46 (m, 4H), 3.18-2.95 (m, 11H), 2.35-2.17 (m, 2H), 1.82-1.68 (m, 2H), 1.62-1.52 (m, 1H), 0.90 (t, J=7.2 Hz, 3H); MS (ESI) m/z 620.3 (M+H).

S1-14-90:

¹H NMR (400 MHz, CD₃OD) δ 7.18-7.10 (m, 4H), 6.95 (d, J=6.0 Hz, 1H), 4.25 (s, 2H), 4.11-4.04 (m, 1H), 4.00 (s, 1H), 3.40-3.34 (m, 2H), 3.19-2.85 (m, 11H), 2.26-2.12 (m, 2H), 1.57-1.51 (m, 1H); MS (ESI) m/z 578.4 (M+H).

S1-14-91:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=5.6 Hz, 1H), 4.34 (s, 2H), 4.13 (s, 1H), 3.20-2.89 (m, 11H), 2.31-2.25 (m, 2H), 1.90-1.73 (m, 6H), 1.60-1.48 (m, 3H), 0.86 (t, J=7.2 Hz, 3H); MS (ESI) m/z 558.1 (M+H).

S1-14-92:

¹H NMR (400 MHz, CD₃OD) δ 7.04 (d, J=5.6 Hz, 1H), 4.38 (s, 2H), 4.03 (s, 1H), 3.18-2.85 (m, 13H), 2.30-2.15 (m, 2H), 1.90-1.75 (m, 6H), 1.70-1.518 (m, 3H), 1.20-1.11 (m, 1H), 0.81 (t, J=7.2 Hz, 3H), 0.78-0.69 (m, 2H), 0.45-0.36 (m, 2H); MS (ESI) m/z 612.1 (M+H).

S1-14-93:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=5.6 Hz, 1H), 4.38-4.34 (m, 2H), 4.03 (s, 1H), 3.36-3.29 (m, 1H), 3.19-2.88 (m, 12H), 2.36-2.25 (m, 2H), 2.00-1.81 (m, 8H), 1.72-1.50 (m, 3H), 0.91 (t, J=7.2 Hz, 3H), 0.82 (t, J=7.2 Hz, 3H); MS (ESI) m/z 600.2 (M+H).

S1-14-94:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=5.6 Hz, 1H), 4.38-4.34 (m, 2H), 4.11 (s, 1H), 3.38-3.30 (m, 1H), 3.25-2.95 (m, 12H), 2.36-2.25 (m, 2H), 2.05-1.85 (m, 6H), 1.78-1.61 (m, 3H), 1.45 (t, J=7.2 Hz, 3H), 0.90 (t, J=7.2 Hz, 3H); MS (ESI) m/z 586.0 (M+H).

S1-14-95:

¹H NMR (400 MHz, CD₃OD) δ 7.01 (d, J=5.2 Hz, 1H), 4.40 (d, J=13.2 Hz, 1H), 4.28 (d, J=13.6 Hz, 1H), 4.04 (s, 1H), 3.22-2.81 (m, 13H), 2.31-2.17 (m, 2H), 2.06-1.93 (m, 2H), 1.82-1.43 (m, 9H), 1.31 (t, J=7.2 Hz, 3H), 1.25-1.14 (m, 3H); MS (ESI) m/z 600.3 (M+H).

S1-14-96:

¹H NMR (400 MHz, CD₃OD) δ 7.02 (d, J=6 Hz, 1H), 4.42-4.29 (m, 2H), 4.05 (s, 1H), 3.22-2.81 (m, 13H), 2.31-2.17 (m, 2H), 2.06-1.93 (m, 2H), 1.82-1.43 (m, 11H), 1.31 (t, J=7.2 Hz, 3H), 1.25-1.14 (m, 3H); MS (ESI) m/z 600.3 (M+H).

S1-14-97:

¹H NMR (400 MHz, CD₃OD) δ 7.15 (d, J=5.2 Hz, 1H), 4.54 (s, 2H), 4.16 (s, 1H), 3.23-3.01 (m, 13H), 2.42-2.29 (m, 2H), 2.14-2.05 (m, 1H), 1.92-1.83 (m, 1H), 1.79-1.45 (m, 9H), 1.48-1.21 (m, 4H), 0.86-0.75 (m, 2H), 0.55-0.47 (m, 2H); MS (ESI) m/z 626.2 (M+H).

S1-14-98:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=5.6 Hz, 1H), 4.29 (s, 2H), 4.13 (s, 1H), 3.21-2.96 (m, 11H), 2.38-2.25 (m, 2H), 2.02-1.91 (m, 1H), 1.85-1.47 (m, 11H), 1.37-1.26 (m, 2H); MS (ESI) m/z 572.2 (M+H).

S1-14-99:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (d, J=5.6 Hz, 1H), 4.31 (s, 2H), 4.00 (s, 1H), 3.57-3.44 (m, 3H), 3.27-3.22 (m, 2H), 3.13-2.85 (m, 10H), 2.28-2.21 (m, 1H), 2.15-2.08 (m, 2H), 2.04-1.89 (m, 3H), 1.85-1.77 (m, 1H), 1.70-1.66 (m, 1H), 1.60-1.48 (m, 1H); MS (ESI) m/z 599.2 (M+H).

S1-14-100:

¹H NMR (400 MHz, CD₃OD) δ 7.03 (d, J=6.0 Hz, 1H), 4.29 (s, 2H), 4.03 (s, 1H), 3.40-3.29 (m, 1H), 3.23-2.81 (m, 10H), 2.63-2.56 (m, 1H), 2.32-2.07 (m, 4H), 1.62-1.49 (m, 2H), 1.35-1.25 (m, 1H), 1.10 (d, J=7.6 Hz, 3H), 0.90 (d, J=7.6 Hz, 3H), 0.89-0.78 (m, 1H); MS (ESI) m/z 558.3 (M+H).

S1-14-101:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=5.6 Hz, 1H), 4.37 (s, 2H), 4.11 (s, 1H), 3.43-3.37 (m, 2H), 3.23-2.97 (m, 9H), 2.68-2.60 (m, 2H), 2.42-2.24 (m, 2H), 1.98-1.81 (m, 3H), 1.71-1.58 (m, 1H), 0.99 (d, J=7.6 Hz, 6H), 0.95-0.85 (m, 1H); MS (ESI) m/z 558.6 (M+H).

S1-14-102:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=6.0 Hz, 1H), 4.53-4.48 (m, 2H), 4.42-4.38 (m, 1H), 4.10 (s, 1H), 3.92-3.62 (m, 2H), 3.55-3.50 (m, 2H), 3.25-2.96 (m, 9H), 2.62-2.51 (m, 1H), 2.40-2.21 (m, 2H), 2.19-2.02 (m, 2H), 1.96 (d, J=7.2 Hz, 3H), 1.71-1.60 (m, 1H); MS (ESI) m/z 573.3 (M+H).

S1-14-103:

¹H NMR (400 MHz, CD₃OD) δ 7.12 (d, J=60 Hz, 1H), 4.57-4.46 (m, 3H), 4.15 (s, 1H), 3.90-3.83 (m, 1H), 3.68-3.51 (m, 3H), 3.24-2.98 (m, 9H), 2.62-2.55 (m, 1H), 2.39-2.25 (m, 2H), 2.21-2.05 (m, 2H), 1.98 (d, J=10 Hz, 3H), 1.67-1.60 (m, 1H); MS (ESI) m/z 572.9 (M+H).

S1-14-104:

¹H NMR (400 MHz, CD₃OD) δ 7.22-7.16 (m, 4H), 7.02 (d, J=6.0 Hz, 1H), 4.52-4.38 (m, 3H), 4.05 (s, 1H), 3.45-3.32 (m, 4H), 3.18-2.95 (m, 11H), 2.32-2.15 (m, 2H), 1.61-1.52 (m, 1H), 1.15-1.08 (m, 1H), 0.73-0.67 (m, 2H), 0.39-0.33 (m, 2H); MS (ESI) m/z 632.0 (M+H).

S1-14-105:

¹H NMR (400 MHz, CD₃OD) δ 7.44-7.37 (m, 5H), 6.93 (d, J=5.6 Hz, 1H), 4.23 (s, 2H), 4.21 (s, 2H), 4.03 (s, 1H), 3.25-2.88 (m, 9H), 2.27-2.15 (m, 2H), 1.59-1.50 (m, 1H); MS (ESI) m/z 552.0 (M+H).

S1-14-106:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (d, J=5.6 Hz, 1H), 4.34 (s, 2H), 4.05 (d, J=8.8 Hz, 2H), 4.02 (s, 1H), 3.25-2.88 (m, 9H), 2.30-2.15 (m, 2H), 1.61-1.51 (m, 1H); MS (ESI) m/z 544.1 (M+H).

S1-14-107:

¹H NMR (400 MHz, CD₃OD) δ 7.03 (d, J=5.2 Hz, 1H), 4.39 (s, 2H), 4.04 (s, 1H), 3.58-3.49 (m, 1H), 3.48-3.44 (m, 1H), 3.18-2.88 (m, 11H), 2.32-2.15 (m, 2H), 1.98-1.88 (m, 1H), 1.82-1.73 (m, 1H), 1.62-1.41 (m, 5H), 0.82 (m, J=7.2 Hz, 3H), 0.78 (m, J=7.2 Hz 3H); MS (ESI) m/z 572.1 (M+H).

S1-14-108:

¹H NMR (400 MHz, CD₃OD) δ 6.99 (t, J=6.0 Hz, 1H), 4.29-4.28 (m, 2H), 4.01 (s, 1H), 4.00-3.98 (m, 1H), 3.33-3.29 (m, 2H), 3.18-2.95 (m, 11H), 2.31-2.27 (m, 2H), 2.17-2.04 (m, 2H), 1.95-1.84 (m, 2H), 1.57-1.49 (m, 1H); MS (ESI) m/z 546.9 (M+H).

S1-14-109:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (t, J=5.6 Hz, 1H), 4.30-4.28 (m, 2H), 4.01 (s, 1H), 4.00-3.98 (m, 1H), 3.55-3.48 (m, 2H), 3.39-3.37 (m, 1H), 3.26 (s, 3H), 3.18-2.95 (m, 11H), 2.32-2.16 (m, 3H), 2.06-2.02 (m, 1H), 1.85-1.78 (m, 2H), 1.62-1.50 (m, 1H); MS (ESI) m/z 560.0 (M+H).

S1-14-110:

¹H NMR (400 MHz, CD₃OD) δ 7.16 (d, J=6.0 Hz, 1H), 4.55 (d, J=13.2 Hz, 1H), 4.39 (d, J=13.2 Hz, 1H), 4.10 (s, 1H), 3.51-3.43 (m, 1H), 3.04 (s, 3H), 2.96 (s, 3H), 2.89 (s, 3H), 3.25-2.97 (m, 3H), 2.35 (t, J=14.7 Hz, 1H), 2.29-2.21 (m, 1H), 1.69-1.59 (m, 1H), 1.38 (d, J=6.9 Hz, 3H), 1.00 (s, 9H); MS (ESI) in m/z 560.25 (M+H).

S1-14-111:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=6.5 Hz, 1H), 4.55 (d, J=12.2 Hz, 1H), 4.44 (d, J=12.2 Hz, 1H), 4.12 (s, 1H), 2.95-3.05 (m, 13H), 2.22-2.40 (m, 2H), 1.59-1.75 (m, 3H), 1.35-1.45 (m, 2H), 1.12-1.25 (m, 1H), 0.95 (t, J=7.7 Hz, 3H), 0.75-0.85 (m, 2H), 0.40-0.50 (m, 2H); MS (ESI) m/z 572.2 (M+H).

S1-14-112:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (d, J=5.6 Hz, 1H), 4.35 (s, 2H), 4.00 (s, 1H), 2.70-3.20 (m, 12H), 2.12-2.31 (m, 2H), 1.50-1.80 (m, 6H), 1.15-1.40 (m, 2H), 0.85-0.95 (m, 6H); MS (ESI) m/z 560.1.

S1-14-113:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=6.0 Hz, 1H), 4.30 (s, 2H), 4.06 (s, 1H), 3.23-3.16 (m, 1H), 3.17-2.95 (m, 9H), 2.39-2.30 (m, 2H), 2.26-2.19 (m, 1H), 1.90-1.81 (m, 1H), 1.78 (t, J=4.6, 1H), 1.67-1.54 (m, 4H), 1.42-1.33 (m, 1H), 1.21 (dd, J=13.7, 4.1 Hz, 1H), 1.01 (s, 3H), 0.93 (s, 3H), 0.89 (s, 3H); MS (ESI) m/z 598.33 (M+H).

S1-14-114:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=6.0 Hz, 1H), 4.37 (d, J=13.3 Hz, 1H), 4.20 (d, J=13.3 Hz, 1H), 4.09 (s, 1H), 3.23-3.16 (m, 1H), 3.17-2.96 (m, 9H), 2.35 (t, J=14.7 Hz, 1H), 2.28-2.21 (m, 1H), 2.13-2.06 (m, 1H), 1.92-1.77 (m, 4H), 1.74-1.60 (m, 2H), 1.24-1.17 (m, 1H), 1.08 (s, 3H), 0.98 (s, 3H), 0.92 (s, 3H); MS (ESI) m/z 598.28 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.11 (d, J=5.2 Hz, 1H), 4.37 (s, 2H), 4.07 (s, 1H), 3.66-3.55 (m, 3H), 3.19-2.92 (m, 11H), 2.84 (s, 6H), 2.40-1.98 (m, 6H), 1.63-1.53 (m, 1H); MS (ESI) m/z 573.1 (M+H)

¹H NMR (400 MHz, CD₃OD) δ 6.96 (d, J=5.6 Hz, 1H), 4.44-4.33 (m, 2H), 4.19 (t, J=8.4 Hz, 1H), 4.02 (s, 1H), 3.59-3.57 (m, 1H), 3.40-3.30 (m, 1H), 3.14-3.11 (m, 2H), 3.03-2.88 (m, 7H), 2.56-2.50 (m, 1H), 2.28-2.20 (m, 1H), 2.17-2.13 (m, 2H), 1.97-1.90 (m, 2H), 1.60-1.51 (m, 1H); MS (ESI) m/z 559.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.11 (d, J=5.2 Hz, 1H), 4.36 (s, 2H), 4.13 (s, 1H), 3.56 (d, J=11.6 Hz, 2H), 3.28 (s, 3H), 3.26 (d, J=5.6 Hz, 2H), 3.22-2.97 (m, 11H), 2.35-2.25 (m, 2H), 1.98-1.90 (m, 3H), 1.67-1.56 (m, 3H); MS (ESI) m/z 574.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.30-7.23 (m, 2H), 7.14 (d, J=4.4 Hz, 1H), 7.01-6.91 (m, 3H), 4.44 (s, 2H), 4.11 (s, 1H), 3.64 (d, J=12 Hz, 1H), 3.45 (s, 3H), 3.23-3.18 (m, 1H), 3.11-2.96 (m, 9H), 2.36-2.16 (m, 6H), 1.68-1.61 (m, 1H); MS (ESI) m/z 622.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.49 (s, 2H), 7.19 (d, J=5.6 Hz, 1H), 4.46 (s, 2H), 4.13 (s, 1H), 3.72 (d, J=12 Hz, 2H), 3.53-3.49 (m, 1H), 3.41-3.35 (m, 2H), 3.23-2.98 (m, 9H), 2.39-2.24 (m, 6H), 1.68-1.58 (m, 1H); MS (ESI) m/z 596.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.15-7.11 (m, 1H), 4.49 (d, J=9.2 Hz, 2H), 4.11 (s, 1H), 3.64-3.60 (m, 1H), 3.49-3.34 (m, 1H), 3.34-3.33 (m, 1H), 3.22-2.96 (m, 10H), 2.60-2.51 (m, 1H), 2.43-2.20 (m, 3H), 1.78-1.50 (m, 7H), 1.42-1.40 (m, 2H); MS (ESI) m/z 570.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.13-7.12 (m, 1H), 4.48 (s, 2H), 4.15 (s, 1H), 3.74-3.56 (m, 2H), 3.46-3.37 (m, 3H), 3.28-3.17 (m, 2H), 3.11-2.97 (m, 8H), 2.82 (d, J=12.8 Hz, 3H), 2.33-2.26 (m, 2H), 2.17-1.92 (m, 5H), 1.75 (d, J=14.4 Hz, 1H), 1.67-1.68 (m, 1H); MS (ESI) m/z 613.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.13 (s, 1H), 4.36 (s, 2H), 4.15 (s, 1H), 3.53 (d, J=11.2 Hz, 2H), 3.25-2.98 (m, 11H), 2.29 (d, J=13.2 Hz, 2H), 1.94 (d, J=14 Hz, 2H), 1.75-1.51 (m, 4H), 1.37-1.23 (m, 4H), 0.91-0.88 (t, J=6.8 Hz, 3H); MS (ESI) m/z 572.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.16 (d, =5.6 Hz, 1H), 4.42 (s, 1H), 4.42 (s, 2H), 4.14 (s, 1H), 3.63 (d, J=11.2 Hz, 2H), 3.25-3.12 (m, 6H), 3.07-2.93 (m, 13H), 2.40-2.30 (m, 3H), 2.11 (d, J=14 Hz, 2H), 1.74-1.65 (m, 3H), MS (ESI) m/z 587.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.96 (d, J=4.8 Hz, 1H), 4.59-4.57 (m, 1H), 4.41-4.31 (m, 2H), 4.04-3.93 (m, 3H), 3.13-2.88 (m, 9H), 2.50-2.46 (m, 1H), 2.28-2.16 (m, 3H), 1.61-1.50 (m, 1H), 1.34 (d, J=4.8 Hz, 3H); MS (ESI) m/z 516.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.14 (d, J=5.6 Hz, 1H), 5.04-5.02 (m, 0.5H), 4.90-4.88 (m, 0.5H), 4.41 (s, 2H), 4.12 (s, 1H), 3.65-3.33 (m, 4H), 3.25-2.97 (m, 9H), 2.37-2.03 (m, 6H), 1.67-1.58 (m, 1H); MS (ESI) m/z 548.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.11 (d, J=5.6 Hz, 1H), 4.38 (s, 2H), 4.11 (s, 1H), 3.45-3.42 (m, 2H), 3.22-2.97 (m, 11H), 2.36-2.24 (m, 2H), 1.85-1.54 (m, 11H), 1.50-1.45 (m, 2H); MS (ESI) m/z 584.3 (M+H)

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=5.6 Hz, 1H), 4.38 (s, 2H), 4.11 (s, 1H), 3.40-3.30 (m, 2H), 3.23-2.97 (m, 11H), 2.36-2.24 (m, 2H), 1.90-1.87 (m, 2H), 1.64-1.32 (m, 13H); MS (ESI) m/z 597.9 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.11 (d, J=5.6 Hz, 1H), 4.41 (s, 2H), 4.11 (s, 1H), 3.54-3.51 (m, 2H), 3.22-2.97 (m, 11H), 2.37-2.18 (m, 4H), 1.68-1.62 (m, 1H), 1.22-1.19 (m, 2H), 0.53-0.50 (m, 4H); MS (ESI) m/z 556.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.15 (d, J=5.6 Hz, 1H), 4.47 (s, 2H), 4.11 (s, 1H), 3.66-3.50 (m, 2H), 3.46-3.32 (m, 2H), 3.22-2.97 (m, 9H), 2.38-2.24 (m, 6H), 1.68-1.58 (m, 1H); MS (ESI) m/z 566.2 (M+H).

¹H NMR (400 MHz, MeOD) δ 7.11 and 7.10 (each s, total 1H), 4.50 (s, 2H), 4.13 (s, 1H), 3.73-3.64 (m, 2H), 3.27-2.95 (m, 9H), 2.40-2.25 (m, 4H), 1.79-1.75 (m, 2H), 1.70-1.60 (m, 1H), 1.42 (d, J=7.2 Hz, 6H); MS (ESI) m/z 544.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.04 (d, J=5.6 Hz, 1H), 4.30 (s, 2H), 4.05 (s, 1H), 3.29-3.22 (m, 4H), 3.13-2.89 (m, 9H), 2.27-2.17 (m, 2H), 1.85-1.80 (m, 2H), 1.72-1.68 (m, 2H), 1.60-1.51 (m, 1H), 1.19 (s, 3H); MS (ESI) m/z 560.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.08 (dd, J=11.0, 5.5 Hz, 1H), 4.61 (dd, J=26.7, 14.2 Hz, 1H), 4.47-4.27 (m, 2H), 4.10 (s, 1H), 4.00-3.82 (m, 2H), 3.43-3.30 (m, 2H), 3.25-2.94 (m, 11H), 2.42-2.32 (m, 1H), 2.29-2.11 (m, 2H), 2.03-1.90 (m, 2H), 1.71-1.56 (m, 2H), 1.38 (t, J=7.3 Hz, 3H); MS (ESI) m/z 574.20 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.53 (d, J=7.6 Hz, 1H), 7.36 (d, J=8.0 Hz, 1H), 7.19 (s, 1H), 7.11 (t, J=7.6 Hz, 1H), 7.04-7.00 (m, 2H), 4.33-4.26 (m, 2H), 4.11 (s, 1H), 3.42-3.37 (m, 2H), 3.26-2.95 (m, 11H), 2.33-2.23 (m, 2H), 1.68-1.59 (m, 1H); MS (ESI) m/z 605.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.00 (d, J=5.6 Hz, 1H), 4.24 (s, 2H), 4.02 (s, 1H), 3.45-3.42 (m, 2H), 3.11-2.87 (m, 11H), 2.26-2.15 (m, 3H), 1.93-1.89 (m, 2H), 1.60-1.48 (m, 3H), 0.80-0.69 (m, 1H), 0.50-0.40 (m, 1H), 0.35-0.33 (m, 2H), 0.07-0.02 (m, 2H); MS (ESI) m/z 570.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.03 (d, J=5.6 Hz, 1H), 4.41 (s, 2H), 4.09 (s, 1H), 3.79-3.66 (m, 1H), 3.61-3.52 (m, 1H), 3.46-3.37 (m, 2H), 3.26-2.93 (m, 10H), 2.43-2.33 (m, 1H), 2.27-2.20 (m, 1H), 2.18-2.05 (m, 2H), 1.95-1.72 (m, 2H), 1.72-1.60 (m, 1H); MS (ESI) m/z 548.14 (M+H).

¹H NMR (400 MHz, CD₃(D) δ 6.98 (d, J=5.6 Hz, 1H), 4.26 (s, 2H), 4.00 (s, 1H), 3.47-3.44 (m, 2H), 3.15-2.89 (m, 11H), 2.32-2.25 (m, 1H), 2.17-2.14 (m, 1H), 1.95-1.92 (m, 3H), 1.76-1.66 (m, 2H), 1.64-1.02 (m, 10H); MS (ESI) m/z 598.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.05 (d, J=5.9 Hz, 1H), 4.31 (s, 2H), 4.09 (s, 1H), 3.95-3.85 (m, 2H), 3.75 (t, J=7.3 Hz, 1H), 3.54 (dd, J=8.7, 5.5 Hz, 1H), 3.20-2.90 (m, 11H), 2.70-2.59 (m, 1H), 2.40-2.30 (m, 1H), 2.28-2.13 (m, 2H), 1.75-1.59 (m, 2H); MS (ESI) m/z 546.16 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.04 (d, J=5.9 Hz, 1H), 4.30 (s, 2H), 4.18-3.94 (m, 4H), 3.90-3.80 (m, 1H), 3.80-3.65 (m, 1H), 3.24-2.85 (m, 9H), 2.50-2.20 (m, 3H), 2.18-2.00 (m, 1H), 1.70-1.57 (m, 1H); MS (ESI) m/z 532.11 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.01 (d, J=5.9 Hz, 1H), 4.31 (d, J=5.0 Hz, 2H), 4.23-4.15 (m, 1H), 4.08 (s, 1H), 3.95-3.88 (m, 1H), 3.85-3.78 (m, 1H), 3.25-3.16 (m, 1H), 3.15-2.93 (m, 9H), 2.39-2.29 (m, 1H), 2.27-2.19 (m, 1H), 2.17-2.07 (m, 1H), 2.00-1.91 (m, 2H), 1.70-1.56 (m, 2H); MS (ESI) m/z 546.13 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.05 (d, J=5.5 Hz, 1H), 4.28 (s, 2H), 4.10 (s, 1H), 3.24-2.83 (m, 11H), 2.54-2.40 (m, 3H), 2.39-2.30 (m, 1H), 2.28-2.21 (m, 1H), 2.11-1.90 (m, 5H), 1.70-1.50 (m, 2H), 1.23 (s, 3H), 1.01 (s, 3H); MS (ESI) m/z 598.19 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.09 (d, J=6.0 Hz, 1H), 4.51 (d, J=1.7 Hz, 2H), 4.09 (s, 1H), 3.78 (t, J=4.8 Hz, 2H) 3.46-3.40 (m, 5H), 3.24-2.94 (m, 11H), 2.37 (t, J=14.7 Hz, 1H), 2.28-2.17 (m, 2H), 1.72-1.60 (m, 1H), 1.09-1.01 (m, 6H); MS (ESI) m/z 576.24 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.09 (d, J=5.9 Hz, 1H), 4.55 (d, J=3.7 Hz, 2H), 4.07 (s, 1H), 3.77 (t, J=4.8 Hz, 2H), 3.66-3.54 (m, 1H), 3.41 (s, 3H), 3.48-3.37 (m, 1H), 3.25-2.90 (m, 11H), 2.43-2.33 (m, 1H), 2.28-2.20 (m, 1H), 1.71-1.60 (m, 1H), 1.25-1.15 (m, 1H), 1.85-1.75 (m, 2H), 0.50-0.42 (m, 2H); MS (ESI) m/z 574.21 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.14 (d, J=6.0 Hz, 1H), 4.56 (br, 2H), 4.09 (s, 1H), 3.80 (br, 2H), 3.55-3.46 (m, 2H), 3.42 (s, 3H), 3.44-3.39 (m, 1H), 3.22-2.92 (m, 10H), 2.42-2.33 (m, 1H), 2.27-2.20 (m, 1H), 1.72-1.60 (m, 1H), 1.09 (s, 9H); MS (ESI) m/z 590.21 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=5.9 Hz, 1H), 4.64-4.52 (m, 1H), 4.38-4.22 (m, 1H), 4.10 (s, 1H), 3.98-3.82 (m, 2H), 3.76 (t, J=7.3 Hz, 1H), 3.55-3.45 (m, 1H), 3.27-2.88 (m, 14H), 2.85-2.73 (m, 1H), 2.42-2.32 (m, 1H), 2.28-2.19 (m, 2H), 1.77-1.59 (m, 2H); MS (ESI) m/z 560.21 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=5.9 Hz, 1H), 4.55-4.39 (m, 2H), 4.08 (s, 1H), 3.98-3.82 (m, 2H), 3.75 (t, J=7.3 Hz, 1H), 3.53-3.40 (m, 1H), 3.36-2.92 (m, 13H), 2.80-2.71 (m, 1H), 2.43-2.33 (m, 1H), 2.28-2.18 (m, 2H), 1.73-1.59 (m, 2H), 1.40 (t, J=7.3 Hz, 3H); MS (ESI) m/z 574.28 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=5.9 Hz, 1H), 4.60-4.40 (m, 2H), 4.09 (s, 1H), 4.02-3.94 (m, 1H), 3.92-3.84 (m, 1H), 3.83-3.73 (m, 1H), 3.52-3.43 (m, 1H), 3.36-2.92 (m, 13H), 2.83-2.71 (m, 1H), 2.43-2.34 (m, 1H), 2.33-2.18 (m, 3H), 1.75-1.60 (m, 2H), 1.06 (dd, J=13.7, 6.9 Hz, 6H); MS (ESI) m/z 602.30 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.09 (d, J=6.0 Hz, 1H), 4.60-4.42 (m, 1H), 4.36-4.22 (m, 2H), 4.22-4.09 (m, 2H), 4.10 (s, 1H), 3.84 (dd, J=11.4, 6.0 Hz, 1H), 3.78-3.67 (m, 1H), 3.24-3.17 (m, 1H), 3.17-2.92 (m, 8H), 2.83 (s, 3H), 2.54-2.20 (m, 4H), 1.70-1.59 (m, 1H); MS (ESI) m/z 546.14 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=6.0 Hz, 1H), 4.48 (d, J=13.7 Hz, 1H), 4.43-4.11 (m, 4H), 4.09 (s, 1H), 3.90-3.78 (m, 1H), 3.76-3.64 (m, 1H), 3.40-3.27 (m, 2H), 3.24-3.18 (m, 1H), 3.17-2.92 (m, 8H), 2.50-2.20 (m, 4H), 1.71-1.59 (m, 1H), 1.45-1.32 (m, 3H); MS (ESI) m/z 560.19 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.06 (dd, J=11.9, 5.9 Hz, 1H), 4.63 (t, J=11.7 Hz, 1H), 4.44-4.25 (m, 2H), 4.09 (s, 1H), 4.00-3.82 (m, 2H), 3.47-3.39 (m, 1H), 3.25-2.87 (m, 11H), 2.42-2.31 (m, 1H), 2.27-2.10 (m, 2H), 2.02-1.90 (m, 2H), 1.71-1.56 (m, 2H); MS (ESI) m/z 560.18 (M+H).

Example 2. Synthesis of Compounds Via Scheme 2

The following compounds were prepared according to Scheme 2.

Compound S1-7 (0.25 g, 0.56 mmol, 1.0 equiv) was dissolved in 1,2-dichloroethane (4 mL). Neopentylamine (98 mg, 0.11 mmol, 2.0 equiv) was added via a syringe, followed by the addition of acetic acid (64 μL, 0.11 mmol) under a nitrogen atmosphere. After stirring at rt for 1 h, sodium triacetoxyborohydride (0.36 g, 1.68 mmol, 3.0 equiv) was added to the reaction mixture. LC/MS indicated that the starting material was consumed after overnight. The reaction mixture was diluted with dichloromethane, washed with saturated aqueous NaHCO₃ (3×20 mL) and brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure to give the crude product, which was purified by column chromatography (Biotage 10 g column, 10% to 20% EtOAc in hexanes gradient), yielding pure compound S2-1-1 as a colorless oil (0.25 g, 86%): ¹H NMR (400 MHz, CDCl₃) δ 7.52-7.47 (m, 2H), 7.40-7.33 (m, 6H), 7.25 (t, J=6.9 Hz, 1H), 7.04 (d, J=8.2 Hz, 2H), 5.10 (s, 2H), 4.04 (d, J=2.3 Hz, 2H), 2.35 (d, J=1.8 Hz, 3H), 2.30 (s, 2H), 0.89 (s, 9H).

LDA/THF was prepared by adding n-BuLi (0.29 mL, 1.6 M/hexanes, 0.46 mmol, 3.0 equiv) to diisopropylamine (65 μL, 0.46 mmol, 3.0 equiv) in 3 mL dry THF under a nitrogen atmosphere in a flame dried schenck flask at −78° C. The resulting solution was warmed to −20° C. and stirred for another 15 min. After the LDA solution was cooled down to −78° C., TMEDA (69 μL, 0.46 mmol, 3.0 equiv) was added slowly via a syringe. Compound S2-1-1 (0.10 g, 0.20 mmol, 1.3 equiv) was dissolved in 1 mL dry THF and added into the LDA solution slowly via a syringe. A dark-red color appeared as soon as addition started. After stirring for 10 min, enone S1-9 (74 mg, 0.15 mmol, 1.0 equiv) in 1 mL dry THF was added slowly a via syringe. After 10 min, LC/MS indicated that the enone was consumed and the product present. The reaction mixture was allowed to slowly warm to −20° C. in 1 h. A phosphate buffer solution (pH 7, 10 m L) was added, followed by the addition of 20 mL saturated aqueous ammonium chloride. The resulting mixture was extracted with dichloromethane (3×15 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated under reduced pressure. The resulting orange-red oil was purified by column chromatography (Biotage 10 g column, 10% to 30% EtOAc in hexanes gradient) to yield the desired compound S2-2-1 (90 mg, 65%).

Aqueous HF (0.3 mL, 48-50%) was added to a CH₃CN solution (1.0 mL) of 7 (20 mg) in a plastic vial at 25° C. The reaction was stirred at 25° C. for 18 hrs. The resulting mixture was poured into an aqueous solution (10 mL) of K₂HPO₄ (2 g). The solution was extracted with EtOAc (3×15 mL). The combined EtOAc extracts were dried over sodium sulfate and concentrated to give the crude intermediate.

10% Pd—C (5 mg) was added to a MeOH solution (2 mL) of the above crude intermediate. HCl/MeOH (0.5 mL, 0.5 N) was also added. The reaction mixture was stirred under H₂ (balloon) at 25° C. for 2 hrs and filtered through a pad of Celite. The filtrate was concentrated to give the crude product. The crude product was purified by HPLC on a Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: CH₃CN, sample in 2.0 mL (0.05 N HCl), gradient elution with 0→70% B over 15 min, mass-directed fraction collection] to yield the desired product S2-4-1 as a yellow solid (8 mg, 66%, 2 steps): ¹H NMR (400 MHz, CD₃OD) δ 7.09 (d, J=6.0 Hz, 1H), 4.42 (s, 2H), 4.14 (s, 1H) 3.21 (dd, J=15.5, 4.6 Hz, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 2.92 (s, 2H), 3.17-2.97 (m, 2H), 2.39-2.22 (m, 2H), 1.70-1.58 (m, 1H), 1.06 (s, 9H); MS (ESI) m/z 532.49 (M+H).

The following compounds were prepared according to the methods for S2-4-1, substituting the appropriate amine for isobutylamine.

S2-4-2:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.08 (d, J=5.96 Hz, 1H), 4.27 (s, 2H), 4.16 (s, 1H), 3.51 (hept, J=6.9 Hz, 1H), 3.28-2.94 (m, 9H), 2.38-2.26 (m, 2H), 1.60 (dd, J=13.3, 11.0 Hz, 1H), 1.41 (d, J=6.9 Hz, 6H); MS (ESI) m/z 504.28 (M+H).

S2-4-3:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.09 (d, J=5.92 Hz, 1H), 4.22 (s, 2H), 4.16 (s, 1H), 3.28-2.94 (m, 9H), 2.38-2.26 (m, 2H), 1.60 (dd, J=14.0, 11.0 Hz, 1H), 1.47 (s, 9H); MS (ESI) m/z 518.28 (M+H).

S2-4-4:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.07 (d, J=5.96 Hz, 1H), 4.26 (s, 2H), 4.16 (s, 1H), 3.66 (quint, J=6.9 Hz, 1H), 3.34-2.94 (m, 11H), 2.36-2.24 (m, 2H), 2.23-2.12 (m, 2H), 1.90-1.54 (m, 5H); MS (ESI) m/z 530.23 (M+H).

S2-4-5:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.08 (d, J=5.96 Hz, 1H), 4.21 (s, 2H), 4.15 (s, 1H), 3.26-2.96 (m, 9H), 2.36-2.24 (m, 2H), 1.82 (q, J=7.32 Hz, 2H), 1.69-1.55 (m, 1H), 1.42 (s, 6H), 1.02 (d, J=7.32 Hz, 3H); MS (ESI) m/z 532.24 (M+H).

S2-4-6:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.11 (d, J=5.96 Hz, 1H), 4.33 (s, 2H), 4.16 (s, 1H), 3.26-2.94 (m, 9H), 2.36-2.25 (m, 2H), 1.69-1.55 (m, 1H), 1.33 (s, 6H), 1.29-1.20 (m, 1H), 0.72-0.64 (m, 2H), 0.63-0.56 (m, 2H); MS (ESI) m/z 544.24 (M+H).

S2-4-7:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.11 (d, J=5.48 Hz, 1H), 4.24 (s, 2H), 4.18 (s, 1H), 3.28-2.96 (m, 9H), 2.37-2.26 (m, 2H), 1.84 (s, 2H), 1.69-1.54 (m, 7H), 1.10 (s, 9H); MS (ESI) m/z 574.28 (M+H).

S2-4-8:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.15 (d, J=5.96 Hz, 1H), 4.37 (s, 2H), 4.17 (s, 1H), 3.56-3.48 (m, 2H), 3.40-2.94 (m, 11H), 2.38-2.26 (m, 2H), 1.99-1.78 (m, 5H), 1.70-1.48 (m, 2H); MS (ESI) m/z 530.29 (M+H).

S2-4-9:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.17 (d, J=5.96 Hz, 1H), 4.45 (s, 2H), 4.16 (s, 1H), 4.08-3.98 (m, 2H), 3.92-3.80 (m, 2H), 3.52-3.42 (m, 2H), 3.38-2.94 (m, 11H), 2.38-2.25 (m, 2H), 1.70-1.55 (m, 1H); MS (ESI) m/z 532.27 (M+H).

S2-4-10:

¹H NMR (400 MHz, CD₃OD/DCl) δ 4.69 (s, 2H), 4.17 (s, 1H), 4.10-3.98 (m, 2H), 3.95-3.84 (m, 2H), 3.34-2.94 (m, 13H), 2.38-2.25 (m, 2H), 1.71-1.57 (m, 1H); MS (ESI) m/z 610.2, 612.19 (M+H).

S2-4-11:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.15 (br s, 1H), 4.76-4.58 (m, 2H), 4.17 (s, 1H), 3.40-2.92 (m, 11H), 2.60-2.48 (m, 1H), 2.38-2.15 (m, 3H), 1.99-1.82 (m, 2H), 1.82-1.55 (m, 2H), 1.45-1.36 (m, 1H), 1.22-1.14 (m, 1H), 1.10-0.99 (m, 1H), 0.99-0.80 (m, 2H); MS (ESI) m/z 556.20 (M+H).

S2-4-12:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.12 (d, J=5.96 Hz, 1H), 4.56 (d, J=3.2 Hz, 1H), 4.17 (s, 1H), 4.10 (d, J=3.2 Hz, 1H), 3.54-3.45 (m, 2H), 3.26-2.96 (m, 9H), 2.39-2.26 (m, 2H), 2.23-1.95 (m, 4H), 1.70-1.56 (m, 4H), 1.44 (s, 3H); MS (ESI) m/z 544.22 (M+H).

S2-4-13:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=6.0 Hz, 1H), 4.42 (s, 2H), 4.14 (s, 1H), 3.06 (s, 3H), 2.98 (s, 3H), 2.92 (s, 6H), 3.24-2.97 (m, 3H), 2.37-2.25 (m, 2H), 1.70-1.57 (m, 1H); MS (ESI) m/z 490.43 (M+H).

S2-4-14:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=6.0 Hz, 1H), 4.28 (s, 2H), 4.14 (s, 1H), 3.21 (dd, J=15.5, 4.6 Hz, 1H), 3.06 (s, 3H), 2.97 (s, 3H), 2.94 (d, J=6.9 Hz, 2H), 3.17-2.97 (m, 2H), 2.35-2.25 (m, 2H), 2.12-2.02 (m, 1H), 1.70-1.58 (m, 1H), 1.04 (d, J=6.9 Hz, 6H); MS (ESI) m/z 518.47 (M+H).

S2-4-15:

¹H NMR (400 MHz, CD₃OD) δ 7.03 (d, J=6.0 Hz, 1H), 4.28 (s, 2H), 4.12 (s, 1H), 3.21 (dd, J=15.1, 4.6 Hz, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 2.94 (d, J=6.9 Hz, 2H), 3.16-2.98 (m, 4H), 2.38-2.22 (m, 2H), 1.72-1.58 (m, 4H), 0.97 (d, J=6.4 Hz, 6H); MS (ESI) m/z 532.50 (M+H).

S2-4-16:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=5.5 Hz, 1H), 4.37 (s, 2H), 4.10 (s, 1H), 3.50 (m, 1H), 3.27-2.93 (m, 5H), 3.05 (s, 3H), 2.97 (s, 3H), 2.85 (m, 1H), 2.36 (t, J=15.1 Hz, 1H), 2.29-2.21 (m, 1H), 1.99-1.81 (m, 2H), 1.72-1.53 (m, 2H), 1.49-1.38 (m, 1H), 1.12 (s, 3H), 1.02 (s, 3H); MS (ESI) m/z 558.49 (M+H).

S2-4-17:

¹H NMR (400 MHz, CD₃OD) δ 7.01 (d, J=6.0 Hz, 1H), 4.17 (s, 2H), 4.10 (s, 1H), 3.21 (dd, J=15.1, 4.6 Hz, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 2.84 (s, 3H), 3.14-2.98 (m, 2H), 2.21-2.39 (m, 2H), 1.70-1.60 (m, 1H); MS (ESI) m/z 476.31 (M+H).

S2-4-18:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (d, J=6.0 Hz, 1H), 4.14 (s, 2H), 4.10 (s, 1H), 3.84 (m, 1H), 3.21 (dd, J=15.5, 4.6 Hz, 1H), 3.04 (s, 3H), 2.96 (s, 3H), 3.14-2.97 (m, 2H), 2.36 (t, J=14.7 Hz, 1H), 2.40-2.30 (m, 3H), 2.28-2.17 (m, 3H), 1.93 (m, 2H), 1.68-1.58 (m, 1H); MS (ESI) m/z 516.34 (M+H).

S2-4-19:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=5.5 Hz, 1H), 4.35 (s, 2H), 4.12 (s, 1H), 3.51 (m, 2H), 3.24-2.98 (m, 5H), 3.05 (s, 3H), 2.97 (s, 3H), 2.34 (t, J=15.1 Hz, 1H), 2.29-2.21 (m, 1H), 1.96-1.85 (m, 2H), 1.79-1.56 (m, 2H), 1.54-1.40 (m, 2H), 0.99 (d, J=6.4 Hz, 3H); MS (ESI) m/z 544.25 (M+H).

S2-4-20:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=5.5 Hz, 1H), 4.39 (s, 2H), 4.09 (s, 1H), 3.38 (m, 2H), 3.24-2.98 (m, 5H), 3.04 (s, 3H), 2.96 (s, 3H), 2.35 (t, J=15.1 Hz, 1H), 2.27-2.19 (m, 1H), 1.77-1.58 (m, 5H), 1.09 (s, 3H), 1.03 (s, 3H); MS (ESI) m/z 558.29 (M+H).

Compound S2-2-1 (30 mg, 0.033 mmol) was dissolved in 1,2-dichloroethane (2 mL). Formaldehyde solution (12 μL, 0.16 mmol, 5.0 equiv) and acetic acid (9 μL, 0.17 mmol) were added via a syringe under a nitrogen atmosphere. After stirring at rt for 1 h, sodium triacetoxyborohydride (21 mg, 0.16 mmol) was added to the reaction mixture. LC/MS indicated that the starting material was consumed after 2 hrs. The reaction mixture was diluted with dichloromethane, washed with NaHCO₃ (saturated aqueous solution, 3×20 mL) and brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure, yielding 26 mg of crude compound S2-2-2, which was used without further purification.

Aqueous HF (0.3 mL, 48-50%) solution was added to a CH₃CN solution (1.0 mL) of S2-2-2 (crude, 26 mg) in a plastic vial at 25° C. The reaction was stirred at 25° C. for 18 hrs. The resulting mixture was poured into an aqueous solution (10 mL) of K₂HPO₄ (2 g). The solution was extracted with EtOAc (3×15 mL). The combined EtOAc extracts were dried over sodium sulfate and concentrated to give the crude intermediate.

Pd—C (5 mg, 10 wt %) was added to MeOH solution (2 mL) of the crude intermediate. HCl in MeOH (0.5 N, 0.5 mL) was added. The reaction was stirred under H₂ (balloon) at 25° C. for 2 hrs and filtered through a pad of Celite. The filtrate was concentrated, and the crude product was purified by HPLC on a Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: CH₃CN, sample in 2.0 mL (0.05 N HCl), gradient elution with 0→70% B over 15 min, mass-directed fraction collection]. Fractions containing the desired product were collected and freeze-dried, yielding product S2-4-21 as a yellow solid 19 mg (>95% for 3 steps): ¹H NMR (400 MHz, CD₃OD) δ 7.12 (d, J=5.8 Hz, 1H), 4.58 (d, J=13.3 Hz, 1H), 4.36 (d, J=13.3 Hz, 1H), 4.11 (s, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 2.95 (s, 5H), 3.26-3.01 (m, 3H), 2.37 (t, J=14.6 Hz, 1H), 2.29-2.22 (m, 1H), 1.71-1.61 (m, 1H), 1.08 (s, 9H); MS (ESI) m/z 546.37 (M+H).

The following compounds were prepared according to the methods for S2-4-21 above, using either formaldehyde or substituting the appropriate aldehyde for formaldehyde.

S2-4-22:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.13 (d, J=5.48 Hz, 1H), 4.60-4.48 (m, 1H), 4.36-4.26 (m, 1H), 4.17 (s, 1H), 3.40-2.94 (m, 11H), 2.85 (s, 3H), 2.38-2.26 (m, 2H), 1.78-1.56 (m, 4H), 1.00-0.92 (m, 6H); MS (ESI) m/z 546.30 (M+H).

S2-4-23:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.11 (d, J=5.96 Hz, 1H), 4.63-4.52 (m, 1H), 4.33-4.24 (m, 1H), 4.16 (s, 1H), 3.26-2.94 (m, 11H), 2.87 (s, 3H), 2.38-2.26 (m, 3H), 1.70-1.56 (m, 1H), 1.10-1.02 (m, 6H); MS (ESI) m/z 532.31 (M+H).

S2-4-24:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.13 (d, J=5.92 Hz, 1H), 4.60-4.50 (m, 1H), 4.24-4.14 (m, 2H), 3.76-3.66 (m, 1H), 3.26-2.94 (m, 9H), 2.79 (s, 3H), 2.40-2.26 (m, 2H), 1.70-1.58 (m, 1H), 1.50-1.40 (m, 6H); MS (ESI) m/z 518.31 (M+H).

S2-4-25:

¹H NMR (400 MHz, CD₃OD/DCl) 7.10 (d, J=4.1 Hz, 1H), 4.80-4.72 (m, 1H), 4.15 (s, 1H), 4.04-3.96 (m, 1H), 3.26-2.94 (m, 9H), 2.78-2.74 (m, 3H), 2.39-2.25 (m, 2H), 1.72-1.53 (m, 10H); MS (ESI) m/z 532.30 (M+H).

S2-4-26:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.13 (d, J=5.5 Hz, 1H), 4.64-4.46 (m, 2H), 4.17 (s, 1H), 3.26-2.94 (m, 12H), 2.39-2.25 (m, 2H), 1.70-1.56 (m, 1H), 1.14-1.04 (m, 1H), 0.98-0.85 (m, 4H); MS (ESI) m/z 516.22 (M+H).

S2-4-27:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.12 (d, J=5.5 Hz, 1H), 4.62-4.53 (m, 1H), 4.28-4.20 (m, 1H), 4.17 (s, 1H), 3.84-3.74 (m, 1H), 3.26-2.94 (m, 9H), 2.79 (s, 3H), 2.39-2.25 (m, 3H), 2.23-2.13 (m, 1H), 1.97-1.81 (m, 4H), 1.80-1.55 (m, 3H); MS (ESI) m/z 544.27 (M+H).

S2-4-28:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.10 (d, J=5.96 Hz, 1H), 4.80-4.71 (m, 1H), 4.18 (s, 1H), 4.06-3.98 (m, 1H), 3.26-2.95 (m, 9H), 2.76 (s, 3H), 2.37-2.25 (m, 2H), 2.01-1.89 (m, 2H), 1.69-1.54 (m, 1H), 1.51 (s, 3H), 1.48 (s, 3H), 1.07 (t, J=6.4 Hz, 3H); MS (ESI) m/z 546.27 (M+H).

S2-4-29:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.11 (d, J=5.0 Hz, 1H), 4.96-4.88 (m, 1H), 4.16 (s, 1H), 4.10-4.01 (m, 1H), 3.26-2.95 (m, 9H), 2.82 (s, 3H), 2.39-2.25 (m, 2H), 1.70-1.58 (m, 1H), 1.50-1.28 (m, 7H), 0.82-0.59 (m, 4H); MS (ESI) m/z 558.28 (M+H).

S2-4-30:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.10 (d, J=5.5 Hz, 1H), 4.82-4.74 (m, 1H), 4.17 (s, 1H), 4.05-3.97 (m, 1H), 3.28-2.95 (m, 9H), 2.76 (s, 3H), 2.38-2.26 (m, 2H), 2.01-1.80 (m, 2H), 1.76-1.56 (m, 7H), 1.12 (s, 9H); MS (ESI) m/z 588.29 (M+H).

S2-4-31:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.21 (d, J=5.0 Hz, 1H), 4.70-4.61 (m, 1H), 4.32-4.26 (m, 1H), 4.19 (s, 1H), 3.56-3.45 (m, 1H), 3.34-2.95 (m, 10H), 2.40-2.26 (m, 2H), 1.72-1.55 (m, 10H), 1.19 (t, J=7.3 Hz, 3H); MS (ESI) m/z 546.26 (M+H).

S2-4-32:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.12 (d, J=4.1 Hz, 1H), 4.51-4.34 (m, 2H), 4.16 (s, 1H), 3.92-3.82 (m, 1H), 3.34-2.94 (m, 11H), 2.40-2.08 (m, 4H), 1.98-1.79 (m, 4H), 1.78-1.55 (m, 3H), 1.36 (t, J=7.4 Hz, 3H); MS (ESI) m/z 558.29 (M+H).

S2-4-33:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.19 (d, J=5.5 Hz, 1H), 4.72-4.63 (m, 1H), 4.34-4.24 (m, 1H), 4.17 (s, 1H), 3.59-3.50 (m, 1H), 3.26-2.96 (m, 10H), 2.40-2.26 (m, 2H), 2.04-1.93 (m, 2H), 1.70-1.50 (m, 7H), 1.18 (t, J=7.3 Hz, 3H), 1.06 (t, J=7.6 Hz, 3H); MS (ESI) m/n 560.35 (M+H).

S2-4-34:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.15 (d, J=5.5 Hz, 1H), 4.64-4.44 (m, 2H), 4.16 (s, 1H), 3.26-2.94 (m, 1H), 2.93-2.82 (m, 1H), 2.41-2.25 (m, 3H), 1.70-1.56 (m, 1H), 1.20-1.05 (m, 7H), 1.05-0.84 (m, 3H); MS (ESI) m/z 558.27 (M+H).

S2-4-35:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.20 (d, J=3.2 Hz, 1H), 4.60-4.49 (m, 2H), 4.16 (s, 1H), 3.45-3.36 (m, 1H), 3.34-2.94 (m, 12H), 2.40-2.26 (m, 2H), 1.70-1.56 (m, 1H), 1.36-1.26 (m, 1H), 1.07 (s, 9H), 0.86-0.74 (m, 2H), 0.57-0.49 (m, 2H); MS (ESI) m/z 586.33 (M+H).

S2-4-36:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.13 (d, J=5.5 Hz, 1H), 4.54-4.33 (m, 2H), 4.16 (s, 1H), 3.98-3.86 (m, 1H), 3.26-2.94 (m, 11H), 2.40-2.24 (m, 2H), 1.70-1.56 (m, 1H), 1.52-1.35 (m, 6H), 1.20-1.08 (m, 1H), 0.80-0.70 (m, 2H), 0.50-0.40 (m, 2H); MS (ESI) m/z 558.21 (M+H).

S2-4-37:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.18 (d, J=5.96 Hz, 1H), 4.52-4.35 (m, 2H), 4.17 (s, 1H), 3.82-3.70 (m, 1H), 3.26-2.94 (m, 11H), 2.41-2.28 (m, 2H), 2.04-1.93 (m, 1H), 1.71-1.58 (m, 1H), 1.52-1.38 (m, 6H), 1.08-1.02 (d, J=6.9 Hz, 3H), 1.00-0.94 (d, J=6.4 Hz, 3H); MS (ESI) m/z 560.20 (M+H).

S2-4-38:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.11 (d, J=5.5 Hz, 1H), 4.54-4.44 (m, 1H), 4.28-4.22 (m, 1H), 4.15 (s, 1H), 3.80-3.72 (m, 1H), 3.36-2.94 (m, 11H), 2.40-2.26 (m, 2H), 1.88-1.54 (m, 3H), 1.50-1.38 (m, 6H), 1.03-0.94 (m, 3H); MS (ESI) m/z 546.20 (M+H).

S2-4-39:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.17 (d, J=5.5 Hz, 1H), 4.52-4.33 (m, 2H), 4.17 (s, 1H), 3.84-3.72 (m, 1H), 3.36-2.94 (m, 11H), 2.40-2.16 (m, 3H), 1.99-1.83 (m, 2H), 1.75-1.53 (m, 5H), 1.52-1.42 (m, 6H), 1.34-1.15 (m, 2H); MS (ESI) m/z 586.26 (M+H).

S2-4-40:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.14 (d, J=5.5 Hz, 1H), 4.56-4.47 (m, 1H), 4.33-4.24 (m, 1H), 4.17 (s, 1H), 3.84-3.72 (m, 1H), 3.36-2.94 (m, 11H), 2.40-2.25 (m, 2H), 1.75-1.54 (m, 4H), 1.52-1.40 (m, 6H), 1.02-0.88 (m, 6H); MS (ESI) m/z 574.26 (M+H).

S2-4-41:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=5.9 Hz, 1H), 4.64 (br, s, 1H), 4.57 (br, s, 1H), 4.10 (s, 1H), 3.21 (dd, J=15.1, 4.6 Hz, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 3.15-2.89 (m, 5H), 2.36 (m, 1H), 2.29-2.21 (m, 1H), 1.70-1.60 (m, 1H), 1.33-1.23 (m, 1H), 1.10-0.85 (m, 3H), 0.85-0.79 (m, 2H), 0.78-0.67 (m, 1H), 0.53-0.47 (m, 2H); MS (ESI) m/z 556.33 (M+H).

S2-4-42:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=5.5 Hz, 1H), 4.54 (d, J=6.4 Hz, 2H), 4.12 (s, 1H), 3.21 (dd, J=15.1, 4.6 Hz, 1H), 3.17-2.95 (m, 4H), 3.05 (s, 3H), 2.97 (s, 3H), 2.92-2.87 (m, 1H), 2.36 (t, J=13.8 Hz, 1H), 2.29-2.21 (m, 1H), 1.9-1.84 (m, 2H), 1.70-1.60 (m, 1H), 1.03 (t, J=7.3 Hz, 3H), 1.10-0.85 (m, 3H), 0.82-0.68 (m, 1H); MS (ESI) in 544.33 (M+H).

S2-4-43:

¹H NMR (400 MHz, CD₃OD) δ 7.09 (d, J=5.5 Hz, 1H), 4.51 (d, J=13.3 Hz, 1H), 4.39 (d, J=13.3 Hz, 1H), 4.07 (s, 1H), 3.03 (s, 3H), 2.95 (s, 3H), 3.25-2.96 (m, 7H), 2.42-2.33 (m, 1H), 2.26-2.18 (m, 1H), 1.70-1.60 (m, 1H), 1.43 (t, J=7.3 Hz, 3H), 1.06 (s, 9H); MS (ESI) m/z 560.50 (M+H).

S2-4-44:

¹H NMR (400 MHz, CD₃OD) δ 7.09 (d, J=5.5 Hz, 1H), 4.52 (d, J=13.3 Hz, 1H), 4.41 (d, J=13.3 Hz, 1H), 4.07 (s, 1H), 3.03 (s, 3H), 2.96 (s, 3H), 3.25-2.96 (m, 7H), 2.43-2.33 (m, 1H), 2.27-2.19 (m, 1H), 1.98-1.88 (m, 1H), 1.86-1.74 (m, 1H), 1.71-1.61 (m, 1H), 1.08 (s, 9H), 1.01 (t, J=6.9 Hz, 3H); MS (ESI) m/z 574.52 (M+H).

S2-4-45:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=5.5 Hz, 1H), 4.53 (d, J=6.8 Hz, 2H), 4.10 (s, 1H), 3.41 (q, J=7.3 Hz, 2H), 3.21 (dd, J=15.1, 4.6 Hz, 1H), 3.17-2.95 (m, 2H), 3.04 (s, 3H), 2.96 (s, 3H), 2.93-2.85 (m, 1H), 2.36 (t, J=13.8 Hz, 1H), 2.29-2.20 (m, 1H), 1.69-1.59 (m, 1H), 1.46 (t, J=7.3 Hz, 3H), 1.10-0.84 (m, 3H), 0.79-0.69 (m, 1H); MS (ESI) m/z 530.38 (M+H).

S2-4-46:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=5.9 Hz, 1H), 4.58 (d, J=13.3 Hz, 1H), 4.26 (d, J=13.3 Hz, 1H), 4.10 (s, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 2.87 (s, 5H), 3.27-2.96 (m, 3H), 2.43-2.32 (m, 2H), 2.28-2.20 (m, 1H), 2.03-1.91 (m, 2H), 1.78-1.60 (m, 5H), 1.36-1.20 (m, 2H); MS (ESI) m/z 558.40 (M+H).

S-2-4-47:

¹H NMR (400 MHz, CD₃OD) δ 7.06 (d, J=5.9 Hz, 1H), 4.56 (d, J=13.3 Hz, 1H), 4.23 (d, J=13.3 Hz, 1H), 4.09 (s, 1H), 3.04 (s, 3H), 2.97 (s, 3H), 2.86 (s, 5H), 3.27-2.96 (m, 3H), 2.37 (t, J=13.8 Hz, 1H), 2.28-2.20 (m, 1H), 1.96-1.59 (m, 7H), 1.44-1.30 (m, 2H), 1.29-1.19 (m, 1H), 1.13-0.95 (m, 2H); MS (ESI) m/z 572.41 (M+H).

S2-4-48:

¹H NMR (400 MHz, CD₃OD) δ 7.09 (d, J=5.5 Hz, 1H), 4.52 (d, J=13.3 Hz, 1H), 4.41 (d, J=13.3 Hz, 1H), 4.07 (s, 1H), 3.03 (s, 3H), 2.96 (s, 3H), 3.25-2.96 (m, 6H), 2.43-2.33 (m, 1H), 2.27-2.19 (m, 1H), 1.66 (m, 1H), 1.08 (s, 9H), 1.10-0.85 (m, 3H), 0.81-0.71 (m, 1H); MS (ESI) m/z 572.43 (M+H).

S2-4-49:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=6.0 Hz, 1H), 4.50 (d, J=13.7 Hz, 1H), 4.37 (d, J=13.7 Hz, 1H), 4.28 (s, 2H), 4.10 (s, 1H), 3.21 (dd, J=15.5, 4.6 Hz, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 3.17-2.98 (m, 6H), 2.37 (t, J=14.7 Hz, 1H), 2.29-2.12 (m, 2H), 1.93-1.73 (m, 2H), 1.71-1.61 (m, 1H), 1.09-0.98 (m, 9H); MS (ESI) m/z 560.35 (M+H).

S-2-4-50:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=6.0 Hz, 1H), 4.50 (d, J=13.5 Hz, 1H), 4.34 (d, J=13.5 Hz, 1H), 4.28 (s, 2H), 4.10 (s, 1H), 3.04 (s, 3H), 2.96 (s, 3H), 3.27-2.97 (m, 7H), 2.36 (t, J=14.7 Hz, 1H), 2.28-2.12 (m, 2H), 1.70-1.60 (m, 1H), 1.39 (t, J=6.9 Hz, 3H), 1.05 (dd, J=15.1, 6.4 Hz, 6H); MS (ESI) m/z 546.39 (M+H).

S2-4-51:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=6.0 Hz, 1H), 4.41 (d, J=12.4 Hz, 1H), 4.14 (d, J=12.4 Hz, 1H), 4.12 (s, 1H), 3.88 (m, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 3.25-2.97 (m, 3H), 2.70 (s, 3H), 2.43-2.22 (m, 6H), 1.96-1.77 (m, 2H), 1.69-1.59 (m, 1H); MS (ESI) m/z 530.34 (M+H).

S2-4-52:

¹H NMR (400 MHz, CD₃OD) δ 7.09 (d, J=6.0 Hz, 1H), 4.31 (br, s, 2H), 4.14 (s, 1H), 4.00-3.92 (m, 1H), 3.06 (s, 3H), 2.97 (s, 3H), 3.26-2.97 (m, 5H), 2.42-2.22 (m, 6H), 1.92-1.77 (m, 2H), 1.69-1.59 (m, 1H), 1.35 (t, J=6.8 Hz, 3H); MS (ESI) m/z 544.35 (M+H).

S2-4-53:

¹H NMR (400 MHz, CD₃OD) δ 7.11 (d, J=6.0 Hz, 1H), 4.48 (m, 1H), 4.37 (m, 1H), 4.13 (s, 1H), 4.04 (m, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 3.25-2.95 (m, 5H), 2.45-2.13 (m, 6H), 1.89-1.74 (m, 2H), 1.69-1.59 (m, 1H), 1.19-1.11 (m, 1H), 0.81-0.72 (m, 2H), 0.46-0.36 (m, 2H); MS (ESI) m/z 570.39 (M+H).

S2-4-54:

¹H NMR (400 MHz, CD₃OD) δ 7.09 (d, J=5.0 Hz, 1H), 6.48 (t, J=53.6 Hz, 1H), 4.55 (s, 2H), 4.11 (s, 1H), 3.83 (t, J=14.2 Hz, 2H), 3.05 (s, 3H), 3.01 (s, 3H), 2.97 (s, 3H), 3.25-2.98 (m, 5H), 2.37 (t, J=14.7 Hz, 1H), 2.29-2.21 (m, 1H), 1.70-1.60 (m, 1H); MS (ESI) m/z 540.31 (M+H).

S2-4-55:

¹H NMR (400 MHz, CD₃OD) δ 7.11 (br, s, 1H), 6.51 (t, J=53.6 Hz, 1H), 4.64 (s, 2H), 4.12 (s, 1H), 3.87 (t, J=14.2 Hz, 2H), 3.05 (s, 3H), 2.97 (s, 3H), 3.25-2.98 (m, 7H), 2.44-2.22 (m, 2H), 1.70-1.61 (m, 1H), 1.27-1.19 (m, 1H), 0.88-0.80 (m, 2H), 0.55-0.45 (m, 2H); MS (ESI) m/z 580.34 (M+H).

S2-4-56:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=5.0 Hz, 1H), 6.38 (t, J=53.6 Hz, 1H), 4.40 (br, s, 2H), 4.09 (s, 1H), 3.56 (m, 2H), 3.03 (s, 3H), 2.96 (s, 3H), 3.22-2.98 (m, 5H), 2.35 (t, J=14.2 Hz, 1H), 2.28-2.08 (m, 2H), 1.69-1.59 (m, 1H), 1.02 (d, J, =6.9 Hz, 6H); MS (ESI) m/z 582.34 (M+H).

S2-4-57:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (t, J=6.0 Hz, 1H), 4.61 (dd, J=18.3, 13.7 Hz, 1H), 4.18 (dd, J=78.3, 13.7 Hz, 1H), 4.10 (s, 1H), 3.87 (t, J=14.2 Hz, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 2.84 (s, 3H), 3.25-2.98 (m, 3H), 2.42-2.20 (m, 3H), 1.70-1.60 (m, 1H), 1.41 (dd, J=30.2, 6.9 Hz, 3H), 1.13 (dd, J=30.2, 6.9 Hz, 3H), 1.04 (dd, J:=15.6, 6.9 Hz, 3H); MS (ESI) m/z 546.30 (M+H).

S2-4-58:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (m, 1H), 4.08 (s, 1H), 4.61 (dd, J=18.3, 13.7 Hz, 1H), 4.18 (dd, J=78.3, 13.7 Hz, 1H), 3.47 (m, 1H), 3.03 (s, 3H), 2.96 (s, 3H), 3.41-2.97 (m, 5H), 2.42-2.20 (m, 3H), 1.70-1.60 (m, 1H), 1.37 (dd, J=30.2, 6.9 Hz, 3H), 1.29 (t, J=6.9 Hz, 3H), 1.13 (dd, J=30.2, 6.9 Hz, 3H), 1.03 (dd, J=15.6, 6.9 Hz, 3H); MS (ESI) m/z 560.33 (M+H).

S2-4-59:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (t, J=6.0 Hz, 1H), 4.61 (dd, J=18.3, 13.7 Hz, 1H), 4.18 (dd, J=78.3, 13.7 Hz, 1H), 4.10 (s, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 3.27-2.98 (m, 4H), 2.83 (s, 3H), 2.42-2.20 (m, 3H), 1.70-1.60 (m, 1H), 1.41 (dd, J=30.2, 6.9 Hz, 3H), 1.13 (dd, J=30.2, 6.9 Hz, 3H), 1.04 (dd, J=15.6, 6.9 Hz, 3H); MS (ESI) m/z 546.32 (M+H).

S2-4-60:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=5.9 Hz, 1H), 4.75 (d, J=13.3 Hz, 1H), 4.60 (d, J==13.3, 1H), 4.10 (s, 1H), 3.04 (s, 3H), 3.02 (s, 3H), 2.96 (s, 3H), 3.25-2.96 (m, 3H), 2.41-2-20 (m, 2H), 1.70-1.59 (m, 2H), 1.19-1.01 (m, 2H), 0.96-0.88 (m, 1H), 0.86-0.70 (m, 3H), 0.43-0.31 (m, 2H); MS (ESI) m/z 556.31 (M+H).

S2-4-61:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (d, J=5.9 Hz, 1H), 4.75 (d, J=13.3 Hz, 1H), 4.60 (d, J=13.3, 1H), 4.10 (s, 1H), 3.05 (s, 3H), 2.96 (s, 3H), 3.25-2.96 (m, 5H), 2.41-2-20 (m, 2H), 1.70-1.59 (m, 2H), 1.35 (t, J=6.8 Hz, 3H), 1.19-1.01 (m, 2H), 0.96-0.88 (m, 1H), 0.86-0.70 (m, 3H), 0.43-0.31 (m, 2H); MS (ESI) m/z 570.32 (M+H).

S2-4-62:

¹H NMR (400 MHz, CD₃OD) δ 7.17 (d, J=6.0 Hz, 1H), 4.51-4.33 (m, 2H), 4.17 (s, 1H), 3.27-3.19 (m, 1H), 3.08 (s, 3H), 2.99 (s, 3H), 3.17-2.97 (m, 4H), 2.38-2.27 (m, 2H), 2.27-2.18 (m, 1H), 1.99-1.83 (m, 2H), 1.74-1.55 (m, 5H), 1.43 (t, J=7.3 Hz, 3H), 1.32-1.14 (m, 2H); MS (ESI) m/z 572.39 (M+H).

S2-4-63:

¹H NMR (400 MHz, CD₃OD) δ 7.11-7.07 (m, 1H), 4.61 (dd, J=18.3, 13.7 Hz, 1H), 4.29, 4.09 (ABq, J=13.7 Hz, 1H), 4.11 (s, 1H), 3.51-3.43 (m, 1H), 3.03 (s, 3H), 2.96 (s, 3H), 3.41-2.97 (m, 5H), 2.34 (t, J=6.9 Hz, 1H), 2.27-2.20 (m, 1H), 2.06-1.95 (m, 1H), 1.74-1.59 (m, 2H), 1.44 (d, J=6.1 Hz, 3H), 1.29 (t, J=6.9 Hz, 3H), 1.08 (t, J=7.6 Hz, 3H); MS (ESI) m/z 546.38 (M+H).

S2-4-64:

¹H NMR (400 MHz, CD₃OD) δ 7.11 (d, J=6.0 Hz, 1H), 4.80-4.71 (m, 1H), 4.18 (s, 1H), 4.06-3.98 (m, 1H), 3.26-2.95 (m, 9H), 2.76 (s, 3H), 2.42-2.30 (m, 2H), 2.01-1.89 (m, 1H), 1.69-1.54 (m, 1H), 1.51 (s, 3H), 1.48 (s, 3H), 1.02 (d, J=6.4 Hz, 6H); MS (ESI) m/z 560.23 (M+H).

Compound S1-7 (89 mg, 0.20 mmol, 1.0 equiv) and (S)-2-methylpiperidine (60 μL, 0.50 mmol, 2.5 equiv) were dissolved in 1,2-dichloroethane (2 mL). Titanium(IV) isopropoxide (0.18 mL, 0.60 mmol, 3.0 equiv) was added at rt. After stirring at rt overnight, LC/M S indicated that most of the starting material was consumed and the intermediate formed. MeOH (1 mL) and sodium borohydride (40 mg, 1.1 mmol, 5.5 equiv, added in 4 equal portions) were added until the intermediate was completely converted to the product. The reaction mixture was diluted with dichloromethane, washed with saturated aqueous sodium bicarbonate, water (2×20 mL) and brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure. The crude product was purified by column chromatography (Biotage 10 g column, 10% to 50% EtOAc in hexanes gradient), yielding 86 mg (82%) of the desired compound S2-1-3 as a colorless oil.

LDA was prepared by adding n-BuLi (0.24 mL, 1.6 M/hexanes, 0.38 mmol, 3.0 equiv) to diisopropylamine (53 μL, 0.38 mmol, 3.0 equiv) in 2 mL dry THF under a nitrogen atmosphere in a flame dried schenck flask cooled at −78° C. The resulting solution was warmed to −20° C. and stirred for 15 min. After the LDA solution was cooled down to −78° C., TMEDA (57 μL, 0.38 mmol, 3.0 equiv) was added slowly via a syringe, followed by the dropwise addition of compound S2-1-3 (86 mg, 0.16 mmol, 1.3 equiv) in 1 mL dry THF (a dark-red color appeared as soon as addition started). After stirring for 10 min, enone S1-9 (54 mg, 0.13 mmol, 1.0 equiv) in 1 mL dry THF was added slowly via a syringe. After 10 min, LC/MS indicated that the enone was consumed and the product present. The reaction mixture was allowed to slowly warm to −30° C. in 1 h, added with a phosphate buffer (pH 7, 10 mL) and saturated aqueous ammonium chloride (20 mL), and extracted with dichloromethane (3×15 mL). The combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure. The resulting orange-red oil was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 4.0 mL (CH₃CN); gradient: 20→100% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product were collected and concentrated at rt to remove most of the acetonitrile. The resulting mostly aqueous solution was extracted with EtOAc. The EtOAc extract was dried (sodium sulfate) and concentrated to give 26 mg (23%) of the desired compound S2-2-3: ¹H NMR (400 MHz, CDCl₃) δ 16.00 (br, s, 1H), 7.58-7.54 (m, 2H), 7.51-7.47 (m, 2H), 7.40-7.31 (m, 6H), 5.35 (s, 2H), 4.98 (q, J=11.0 Hz, 2H), 4.13-4.07 (m, 1H), 3.93 (d, J=11.0 Hz, 1H), 3.42 (d, J=12.2 Hz, 1H), 3.24 (dd, J=15.9, 4.9 Hz, 1H), 3.03-2.93 (m, 1H), 2.69-2.62 (m, 1H), 2.58-2.51 (m, 1H), 2.48 (s, 6H), 2.50-2.40 (m, 3H), 2.19-2.10 (m, 2H), 1.66-1.58 (m, 2H), 1.48-1.26 (m, 4H), 1.22 (d, J=6.1 Hz, 3H), 0.81 (s, 9H), 0.26 (s, 3H), 0.12 (s, 3H).

Aqueous HF (0.3 mL, 48-50%) was added to a CH₃CN solution (1.0 mL) of S2-2-3 (26 mg) in a plastic vial at 25° C. The reaction was stirred at 25° C. for 18 hrs. The resulting mixture was poured into an aqueous solution (10 mL) of K₂H—PO₄ (2 g). The solution was extracted with EtOAc (3×15 mL). The combined EtOAc extracts were dried over sodium sulfate and concentrated to give the crude intermediate (18 mg).

Pd—C (10 mg, 10 wt %) was added to a MeOH solution (2 mL) of the above crude intermediate. HCl/MeOH (0.5 mL, 0.5 N) was also added. The reaction was stirred under H₂ (balloon) at 25° C. for 2 hrs and filtered through a pad of Celite. The filtrate was concentrated, and the crude product was purified by HPLC on a Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: CH₃CN, sample in 2.0 mL (0.05 N HCl), gradient elution with 0→70% B over 15 min, mass-directed fraction collection] to yield the desired product S2-4-65 as a yellow solid (9 mg, 59%, two steps): ¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=6.0 Hz, 1H), 4.76 (d, J=13.7 Hz, 1H), 4.15 (d, J=13.7 Hz, 1H), 4.10 (s, 1H), 3.40-3.32 (m, 2H), 3.05 (s, 3H), 2.97 (s, 3H), 3.27-2.96 (m, 4H), 2.42-2.32 (m, 1H), 2.27-2.21 (m, 1H), 1.92-1.80 (m, 2H), 1.73-1.58 (m, 3H), 1.56 (d, J=6.4 Hz, 3H); MS (ESI) m/z 544.26 (M+H).

The following compounds were prepared according to the methods of S2-4-65, substituting the appropriate amine for 2-methylpiperidine.

S2-4-66:

¹H NMR (400 MHz, CD₃OD) δ 7.06 (dd, J=8.2, 6.0 Hz, 1H), 4.40 (s, 2H), 4.09 (s, 1H), 3.68-3.57 (m, 2H), 3.24-2.98 (m, 3H), 3.04 (s, 3H), 2.96 (s, 3H), 2.40-2.32 (m, 1H), 2.29-2.21 (m, 1H), 2.05-1.58 (m, 7H), 1.54 (d, J=6.0 Hz, 3H), 1.35 (d, J=6.0 Hz, 3H); MS (ESI) m/z 558.31 (M+H).

S2-4-67:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (d, J=6.0 Hz, 1H), 4.33 (s, 2H), 4.15 (br, s, 2H), 4.09 (s, 1H), 3.04 (s, 3H), 2.97 (s, 3H), 3.23-2.96 (m, 3H), 2.38-2.29 (m, 3H), 2.27-2.20 (m, 1H), 2.08-2.01 (m, 2H), 1.86-1.80 (m, 2H), 1.71-1.61 (m, 1H); MS (ESI) m/z 542.26 (M+H).

Acetic anhydride (3.6 μL, 0.038 mmol, 2.0 equiv) was added to a solution of S2-2-1 (17 mg, 0.019 mmol, 1.0 equiv) in dichloromethane (1 mL). After 2 hrs, the reaction mixture was concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; gradient: 90→100% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 5.0-6.0 min, were collected and concentrated under reduced pressure to give 12 mg (66%) of the desired compound S2-2-4: ¹H NMR (400 MHz, CDCl₃) δ 16.02-15.88 (m, 1H), 7.58-7.44 (m, 4H), 7.40-7.28 (m, 6H), 5.36 (s, 2H), 5.03-4.70 (m, 4H), 3.94 (hr s, 1H), 3.30-3.14 (m, 3H), 3.04-2.93 (m, 1H), 2.64-2.32 (m, 9H), 2.16-2.06 (m, 4H), 1.04-0.94 (m, 9H), 0.81 (s, 9H), 0.24 (s, 3H), 0.12 (s, 3H); MS (ESI) m/z 944.61, 946.61 (M+H).

A solution of compound S2-2-4 (12 mg, 0.013 mmol) in 1,4-dioxane (0.80 mL) was treated with HF (0.40 mL, 48-50% aqueous solution) at rt. After stirring overnight, the mixture was poured into a solution of K₂HPO₄ (4.8 g) in water (20 mL). This mixture was extracted with EtOAc (3 times), and the combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure to yield the crude intermediate.

The above crude intermediate was dissolved in MeOH (1 mL)/1,4-dioxane (1 mL). HCl/MeOH (0.5 mL, 0.5 N) and 10% Pd—C (Degussa, 2 mg) were added, and an atmosphere of hydrogen was introduced. After 2 hrs, the reaction mixture was filtered through Celite and concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: CH₃CN, gradient elution with 0→70% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 9.8-11.4 min, were collected and freeze-dried to yield the desired compound S2-4-68 (5 mg, 69%): ¹H NMR (400 MHz, CD₃OD/DCl) δ 7.14 (d, J=5.5 Hz, 1H), 4.40 (s, 2H), 4.15 (s, 1H), 3.82-3.56 (m, 5H), 3.26-2.94 (m, 9H), 2.36-2.24 (m, 2H), 2.24-1.98 (br m, 9H), 1.70-1.55 (m, 1H); MS (ESI) m/z 574.33 (M+H).

Compound S2-1-2 was prepared from S1-7 using similar procedures to that of S15-15-2. A colorless oil: ¹H NMR (400 MHz, CDCl₃) δ 7.49 (dd, J=2.3, 7.8 Hz, 2H), 7.33-7.38 (m, 5H), 7.25 (t, J=7.8 Hz, 1H), 7.05 (d, J=7.8 Hz, 2H), 5.10 (s, 2H), 3.81 (s, 2H), 3.43 (s, 3H), 2.93 (s, 3H), 2.34 (s, 3H), 2.07 (s, 3H), 1.45 (s, 6H); MS (ESI) m/z 571.2 (M+H), calcd for C₂₉H₃₂BrFN₂O₄ 571.15.

Compound S2-4-69 was prepared from S2-1-2 using similar procedures to that of S2-4-1. The crude product was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 10→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 10.20-10.80 min, were collected and freeze-dried to give the desired product S2-4-69 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.39 (d, J=5.8 Hz, 1H), 4.23 (d, J=12.4 Hz, 1H), 4.15 (d, J=12.4 Hz, 1H), 4.10 (s, 1H), 2.97-3.16 (m, 15H), 2.71 (s, 3H), 2.35 (dd, J=15.1 Hz, 1H), 2.23-2.26 (m, 1H), 1.86 (s, 3H), 1.66 (s, 3H), 1.60-1.70 (m, 1H); MS (ESI) m/z 589.5 (M+H), calcd for C₂₉H₃₈FN₄O₈ 589.26.

Example 3. Synthesis of Compounds Via Scheme 3

The following compounds were prepared according to Scheme 3.

Compound S1-7 (0.42 g, 0.94 mmol, 1.0 equiv) was dissolved in MeOH (5 mL). Sodium borohydride (76 mg, 2.00 mmol, 2.1 equiv) was added in several portions. During the addition, gas evolution was observed. After stirring at rt for 30 min, LC/MS indicated that the starting material was consumed. The reaction mixture was diluted with EtOAc, washed with water (2×20 mL) and brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure. The crude material was purified by column chromatography (Biotage 10 g column, 50% to 20% EtOAc in hexanes gradient), yielding 0.37 g (88%) of the desired compound S3-1 as a colorless oil: ¹H NMR (400 MHz, CDCl₃) δ 10.37 (s, 1H), 7.49 (dd, J=7.8, 2.3 Hz, 2H), 7.40-7.33 (m, 5H), 7.25 (t, J=7.8 Hz, 1H), 7.07-7.02 (m, 2H), 5.10 (s, 2H), 4.91 (dd, J=6.9, 2.3 Hz, 2H), 2.35 (d, J=2.3 Hz, 3H).

LDA was freshly prepared by adding n-BuLi (1.6 M/hexanes, 1.59 mmol, 3.0 equiv) to diisopropylamine (0.22 mL, 1.59 mmol, 3.0 equiv) in 10 mL, dry THF under a nitrogen atmosphere in a flame dried schenck flask at −78° C. The pale solution was warmed to −20° C. and stirred for 15 min. After the LDA solution was cooled down to −78° C. with a dry ice/acetone bath, TMEDA (0.24 mL, 1.59 mmol, 3.0 equiv) was added slowly via a syringe. Compound S3-1 (0.28 g, 0.64 mmol, 1.2 equiv) in 2 mL dry THF was added to slowly via a syringe. A dark-red color appeared as soon as the addition started. After stirring at −78° C. for 10 min, enone S1-9 (0.26 g, 0.53 mmol, 1.0 equiv) 2 mL, dry THF was added slowly via a syringe. After 10 min, LC/MS indicated that the enone was consumed and the product present. The reaction mixture was allowed to slowly warm to −20° C. in 1 h. Phosphate buffer (pH 7, 10 mL) was added, followed by the addition of 20 mL saturated aqueous ammonium chloride. The resulting mixture was extract with dichloromethane (3×15 mL.). The combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure. The resulting orange-red oil was purified by column chromatography (Biotage 24 g column, 10% to 30% EtOAc in hexanes gradient) yielding 0.22 g of compound S3-2 (50%): ¹H NMR (400 MHz, CDCl₃) δ 16.1 (br, s, 1H), 7.58-7.54 (m, 2H), 7.51-7.47 (m, 2H), 7.41-7.32 (m, 6H), 5.36 (s, 2H), 4.95 (dd, J=26.6, 9.6 Hz, 2H), 4.89 (d, J=7.8 Hz, 2H), 3.92 (d, J=10.6 Hz, 1H), 3.25 (dd, J=15.6, 4.1 Hz, 1H), 3.04-2.94 (m, 1H), 2.59-2.53 (m, 1H), 2.49 (s, 6H), 2.40 (t, J=15.6 Hz, 1H), 2.20-2.10 (m, 2H), 0.81 (s, 9H), 0.26 (s, 3H), 0.12 (s, 3H).

Aqueous HF (0.3 mL, 48-50%) solution was added to a CH₃CN solution (1.0 mL) of S3-2 (5 mg, 0.06 mmol) in a plastic vial at 25° C. The reaction was stirred at 25° C. for 18 hrs. The reaction mixture was poured into an aqueous solution (10 mL) of K₂HPO₄ (2 g). The solution was extracted with EtOAc (3×15 mL). The combined EtOAc extracts were dried over sodium sulfate and concentrated to give the crude intermediate.

Pd—C (5 mg, 10 wt %) was added to a MeOH solution (2 mL) of the crude intermediate. HCl in MeOH (0.5 mL, 0.5 N) was added and the reaction was stirred under H₂ (balloon) at 25° C. for 2 hrs. The catalyst was filtered off with a pad of Celite. The filtrate was concentrated to afford the crude product, which was purified by HPLC on a Polymerx 10μ RP-γ□ 100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: CH₃CN, sample in 2.0 mL (0.05 N HCl), gradient elution with 0→70% B over 15 min, mass-directed fraction collection], yielding 2 mg of the desired product S3-5-1 as a yellow solid (54%, two steps): ¹H NMR (400 MHz, CD₃OD) δ 6.94 (d, J=6.0 Hz, 1H), 4.33 (s, 2H), 4.12 (s, 1H), 3.03 (s, 3H), 2.96 (s, 3H), 3.18-2.95 (m, 3H), 2.27 (t, J=16.4 Hz, 1H), 2.24-2.17 (m, 1H), 1.67-1.57 (m, 1H); MS (ESI) m/z 463.37 (M+H).

Methanesulfonic anhydride (99 mg, 0.57 mmol, 3.0 equiv) was added to a solution of S3-2 (0.16 g, 0.19 mmol, 1.0 equiv) in THF (4 mL). After 1 h, triethylamine (0.079 mL, 0.57 mmol, 3.0 equiv) was added. After 1 h, additional methanesulfonic anhydride (0.16 g, 095 mmol, 5.0 equiv) was added. After 1 h, the reaction mixture was used without concentration in subsequent reactions: MS (ESI) m/z 911.45, 913.44 (M+H).

3,3-Dimethylbutylamine (0.063 mL, 0.47 mmol, 10 equiv) was added to a solution of S3-3 in THF (1 mL, 0.047 mmol, 1.0 equiv). After 1 h, additional 3,3-dimethylbutylamine (0.13 mL, 0.95 mmol, 20 equiv) was added. After 30 minutes, the reaction mixture was concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; gradient: 20→100% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 8.2-9.0 min, were collected and freeze-dried to yield 10 mg (22%) of compound S3-4-2: ¹H NMR (400 MHz, CDCl₃) δ 16.10-15.79 (br s, 1H), 7.60-7.45 (m, 4H), 7.43-7.28 (m, 6H), 5.35 (s, 2H), 5.04-4.84 (m, 2H), 3.90 (d, J=11.0 Hz, 1H), 3.32-3.19 (m, 1H), 3.06-2.93 (m, 1H), 2.86-2.76 (m, 1H), 2.60-2.34 (m, 10H), 2.18-2.10 (m, 1H), 1.70-1.58 (m, 4H), 0.99-0.76 (m, 18H), 0.26 (s, 3H), 0.11 (s, 3H); MS (ESI) m/z 916.54, 918.49 (M+H).

A solution of compound S3-4-2 (10 mg, 0.011 mmol) in 1,4-dioxane (0.80 mL) was treated with HF (0.40 mL, 48-50% aqueous solution). After stirring overnight, the mixture was poured into a solution of K₂HPO₄ (4.8 g) in water (20 mL). This mixture was extracted with EtOAc (3 times). The combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure to yield the crude intermediate.

The above crude intermediate was dissolved in MeOH (1 mL), 1,4-dioxane (1 mL), and 0.5 N HCl/MeOH (0.4 mL). 10% Pd—C(Degussa, 2 mg) was added, and an atmosphere of hydrogen was introduced. After 2 hrs, the reaction mixture was filtered through Celite and concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Polymerx 10μ RP-γ100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: CH₃CN, gradient elution with 0→70% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 8.2-9.6 min, were collected and freeze-dried to yield 5 mg (69%) of compound S3-5-2: ¹H NMR (400 MHz, CD₃OD/DCl) δ 7.08 (d, J=5.48 Hz, 1H), 4.29 (s, 2H), 4.16 (s, 1H), 3.34-2.96 (m, 11H), 2.36-2.25 (m, 2H), 1.71-1.58 (m, 3H), 0.97 (s, 9H); MS (ESI) m/z 546.32 (M+H).

The following compounds were prepared according to the methods of S3-5-2, substituting the appropriate amine for 3,3-dimethylbutylamine:

S3-5-3:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.07 (d, J=5.96 Hz, 1H), 4.32 (s, 2H), 4.17 (s, 1H), 3.80-3.65 (m, 3H), 3.36-2.96 (m, 11H), 2.38-2.25 (m, 2H), 1.62 (dd, J=14.0, 11.4 Hz, 1H), 1.18 (d, J=6.4 Hz, 6H); MS (ESI) m/z 548.30 (M+H).

S3-5-4:

¹H NMR (400 MHz, CD₃OD/DCl) δ 6.55 (d, J=5.60 Hz, 0.45H), 6.45 (d, J=5.52 Hz, 0.55H), 4.78 (s, 1.1H), 4.67 (s, 0.9H), 4.15 (s, 1H), 3.40-2.96 (m, 16H), 2.32-2.20 (m, 4H), 2.10 (s, 2H), 1.62 (m, 1H), 1.02 (s, 4H); MS (ESI) m/z 559.33 (M+H).

S3-5-5:

¹H NMR (400 MHz, CD₃OD) δ 7.06 (d, J=6.0 Hz, 1H), 4.46 (s, 2H), 4.09 (s, 1H), 3.04 (s, 3H), 2.97 (s, 3H), 3.27-2.97 (m, 3H), 2.37 (m, 1H), 2.28-2.14 (m, 3H), 2.08-2.00 (m, 2H), 1.72-1.62 (m, 1H); MS (ESI) m/z 516.40 (M+H).

S3-5-6:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.22 (d, J=5.96 Hz, 1H), 4.39 (s, 2H), 4.17 (s, 1H), 3.40 (s, 2H), 3.26-2.90 (m, 17H), 2.40-2.25 (m, 2H), 1.70-1.57 (m, 1H), 1.32 (s, 6H); MS (ESI) m/z 575.37 (M+H).

Example 4. Synthesis of Compounds Via Scheme 4

The following compounds were prepared according to Scheme 4.

Compound S1-7 (0.50 g, 1.14 mmol, 1.0 equiv) and t-butylamine (0.60 mL, 5.68 mmol, 5.0 equiv) were stirred at rt in toluene (5 mL) overnight. The reaction mixture was concentrated under reduced pressure. ¹H NMR indicated a 4:1 mixture of S4-1:S1-7. The material was redissolved in toluene (5 mL). t-Butylamine (0.60 mL, 5.68 mmol, 5.0 equiv) and 4 Å molecular sieves (0.50 g) were added. After stirring at rt overnight, the reaction mixture was filtered through Celite and concentrated to give crude S4-1 (with ˜10% S1-7): ¹H NMR (400 MHz, CDCl₃) δ 8.32 (s, 1H), 7.38 (d, J=6.0 Hz, 2H), 7.45-7.32 (m, 5H), 7.31-7.14 (m, 3H), 5.11 (s, 2H), 2.35 (s, 3H), 1.38 (s, 9H).

A solution of S4-1 (0.56 g, 1.13 mmol, 1.2 equiv) in THE (5 mL) was added to a solution of LM DS (1.4 mL, 1.0 M/THF, 1.40 mmol, 1.5 equiv) and TMEDA (1.02 mL, 6.78 mmol, 6.0 equiv) in THF (10 mL) at −78° C. No color change was observed. Additional LDA (1.22 mL, 1.2 M/THF, 1.47 mmol, 1.3 equiv) was added dropwise, immediately producing a red colored solution. The reaction was stirred at −78° C. for 5 min. A solution of enone S1-9 (0.45 g, 0.94 mmol, 1.0 equiv) in THF (3 mL) was added dropwise to the reaction mixture. The reaction was stirred from −78° C. to −20° C. for 1 h, quenched by saturated aqueous NH₄Cl, and extracted with EtOAc. The combined EtOAc extracts were dried (sodium sulfate) and concentrated to yield the crude product, which was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent 3: CH₃CN with 0.1% HCO₂H; gradient: 90→100% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 5.8-7.4 min, were collected and concentrated under reduced pressure to give 0.23 g (29%) of pure S4-2 (The imine hydrolyzed to the aldehyde on concentration): ¹H NMR (400 MHz, CDCl₃) δ 15.79 (s, 1H), 10.33 (s, 1H), 7.60-7.45 (m, 4H), 7.45-7.30 (m, 6H), 5.28 (s, 2H), 4.98 (q, J=9.2 Hz, 2H), 3.89 (d, J=10.4 Hz, 1H), 3.30-3.22 (m, 1H), 3.08-2.95 (m, 1H), 2.62-2.57 (m, 1H), 2.52-2.32 (m, 8H), 2.20-2.12 (m, 1H), 0.81 (s, 9H), 0.26 (s, 3H), 0.12 (s, 3H); MS (ESI) m/z 831.56, 833.55 (M+H).

Cyclopropylamine (0.030 mL, 0.42 mmol, 7.0 equiv) was added to a solution of S4-2 (50 mg, 0.060 mmol, 1.0 equiv) and acetic acid (0.024 mL, 0.42 mmol, 7.0 equiv) in dichloromethane (1 mL). After 30 min., sodium triacetoxyborohydride (64 mg, 0.30 mmol, 5.0 equiv) was added. After an additional 3 hrs, the mixture was quenched by NaHCO₃ (saturated, aqueous solution) and pH 7 phosphate buffer. The mixture was extracted with dichloromethane, and the combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; gradient: 20→100% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 8.6-10.4 min, were collected and freeze-dried to yield 8 mg of compound S4-3-1 (16%, ˜90% pure by ¹H NMR): ¹H NMR (400 MHz, CDCl₃) δ 16.00-15.79 (br s, 1H), 7.60-7.45 (m, 4H), 7.43-7.26 (m, 6H), 4.97 (q, J=9.4 Hz, 2H), 4.37 (br s, 2H), 3.90 (d, J=10.4 Hz, 1H), 3.30-3.22 (m, 1H), 3.08-2.95 (m, 1H), 2.62-2.27 (m, 12H), 2.18-2.10 (m, 1H), 0.88-0.74 (m, 11H), 0.70-0.62 (m, 2H), 0.26 (s, 3H), 0.12 (s, 3H); MS (ESI) m/z 872.43, 874.41 (M+H).

A solution of compound S4-3-1 (8 mg, 0.0094 mmol) in 1,4-dioxane (1 mL) was treated with HF (0.40 mL, 48-50% aqueous solution). After stirring at rt overnight, the mixture was poured into a solution of K₂HPO₄ (4.8 g) in water (20 mL). This mixture was extracted with EtOAc (3 times), and the combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure to yield the crude product.

The above crude product was dissolved in MeOH (1 mL), 1,4-dioxane (1 mL), and 0.5 N HCl/MeOH (0.5 mL). 10% Pd—C (Degussa, 2 mg) was added, and an atmosphere of hydrogen was introduced. After 2 hrs, the reaction mixture was filtered through (Celite and concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Polymerx 10μ RP-γ100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: CH₃CN, gradient elution with 0→70% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 6.8-7.6 min, were collected and freeze-dried to yield 3 mg (56%) of compound S4-5-1: ¹H NMR (400 MHz, CD₃OD/DCl) δ 7.08 (d, J=5.96 Hz, 1H), 4.38 (s, 2H), 4.18 (s, 1H), 3.34-2.96 (m, 9H), 2.86-2.79 (m, 1H), 2.36-2.25 (m, 2H), 1.62 (dd, J=14.0, 11.0 Hz, 1H), 1.02-0.86 (m, 4H); MS (ESI) m/z 502.22 (M+H).

The following compounds were prepared according to the methods of S4-5-1, substituting the appropriate amines for cyclopropylamine.

S4-5-2:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.11 (d, J=5.48 Hz, 1H), 4.51 (dd, J=13.3, 6.4 Hz, 1H), 4.31 (dd, J=13.3, 6.4 Hz, 1H), 4.16 (s, 1H), 3.42-3.10 (m, 4H), 3.10-2.97 (m, 7H), 2.85 (s, 3H), 2.39-2.25 (m, 2H), 1.62 (dd, J=14.0, 11.0 Hz, 1H), 1.41 (t, J=7.3 Hz, 3H); MS (ESI) m/z 504.22 (M+H).

S4-5-3:

¹H NMR (400 Mt-Hz, CD₃OD/DCl) δ 7.10 (d, J=5.96 Hz, 1H), 4.23 (s, 2H), 4.17 (s, 1H), 3.35-2.96 (m, 13H), 2.40-2.25 (m, 2H), 1.63 (dd, J=14.0, 11.0 Hz, 1H), 1.39 (t, J=7.1 Hz, 6H); MS (ESI) m/z 518.24 (M+H).

S4-5-4:

¹H NMR (400 MHz, CD₃OD/DCl) δ 7.22 (d, J=5.48 Hz, 1H), 4.60 (s, 2H), 4.16 (s, 1H), 3.98-3.60 (br m, 8H), 3.24-2.94 (m, 12H), 2.40-2.24 (m, 2H), 1.64 (dd, J=14.0, 11.0 Hz, 1H); MS (ESI) m/z 545.26 (M+H).

Prepared according to the method of compound S4-3-1 above, substituting 2-methoxyethylamine for cyclopropylamine: ¹H NMR (400 MHz, CDCl₃) δ 16.00-15.79 (br s, 1H), 7.58-7.45 (m, 4H), 7.42-7.30 (m, 6H), 5.36 (s, 2H), 5.02-4.88 (m, 2H), 4.35-4.20 (m, 2H), 3.95-3.88 (m, 1H), 3.75-58 (m, 2H), 3.35 (s, 3H), 3.30-3.19 (m, 1H), 3.08-2.95 (br m, 3H), 2.62-2.36 (m, 9H), 2.18-2.10 (m, 1H), 0.81 (s, 9H), 0.26 (s, 3H), 0.12 (s, 3H); MS (ESI) m/z 890.55, 892.53 (M+H).

Formaldehyde (37% aqueous solution, 0.0092 mL, 0.12 mmol, 5.0 equiv) was added to a solution of compound S4-3-2 (22 mg, 0.025 mmol) in dichloromethane (1 mL) and HOAc (0.0071 mL, 0.12 mmol, 5.0 equiv). After 30 minutes, Na(OAc)₃BH (16 mg, 0.074 mmol, 3.0 equiv) was added. After 2 hrs, the mixture was quenched by NaHCO₃ (saturated, aqueous solution) and pH 7 phosphate buffer. This mixture was extracted with dichloromethane, and the combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure.

The above crude intermediate was dissolved in 1,4-dioxane (0.80 mL) and treated with HF (0.40 mL, 48-500% aqueous solution) at rt. After stirring overnight, the mixture was poured into a solution of K₂HPO₄ (4.8 g) in water (20 mL) and extracted with EtOAc (3 times). The combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure.

The above crude intermediate was dissolved in MeOH (1 mL), 1,4-dioxane (1 mL), and 0.5 N HCl/MeOH (0.5 mL). 10% Pd—C (Degussa, 2 mg) was added, and an atmosphere of hydrogen was introduced. After 2 hrs, the reaction mixture was filtered through Celite and concentrated under reduced pressure to afford the crude product, which was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: CH₃CN, gradient elution with 0→70% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 6.5-7.6 min, were collected and freeze-dried to yield 11 mg of compound S4-5-5 (75%, 3 steps): ¹H NMR (400 MHz, CD₃OD/DCl) δ 7.10 (d, J=5.52 Hz, 1H), 4.59 (dd, J=13.3, 7.8 Hz, 1H), 4.34 (dd, J=13.3, 7.8 Hz, 1H), 4.17 (s, 1H), 3.84-3.72 (m, 2H), 3.54-3.35 (m, 5H), 3.34-2.96 (m, 9H), 2.91 (s, 3H), 2.38-2.25 (m, 2H), 1.70-1.55 (m, 1H); MS (ESI) m/z 534.25 (M+H).

Prepared according to the methods of S4-5-5, substituting cyclopropanemethylamine for 2-methoxyethylamine: ¹H NMR (400 MHz, CD₃OD/DCl) δ 7.12 (d, J=5.52 Hz, 1H), 4.62 (dd, J=13.3, 7.8 Hz, 1H), 4.32 (dd, J=13.3, 7.8 Hz, 1H), 4.16 (s, 1H), 3.35-2.96 (m, 11H), 2.90 (s, 3H), 2.40-2.26 (m, 2H), 1.63 (dd, J=14.0, 11.4 Hz, 1H), 1.30-1.17 (m, 1H), 0.86-0.74 (m, 2H), 0.56-0.43 (m, 2H); MS (ESI) m/z 530.25 (M+H).

Example 5. Synthesis of Compounds Via Scheme 5

The following compounds were prepared according to Scheme 5.

Methylmagnesium bromide (0.51 mL, 3.0 M/Et₂O, 1.53 mmol, 1.0 equiv) was added to a solution of compound S1-7 (0.67 g, 1.52 mmol, 1.0 equiv) in THF (8 mL) at −78° C. After 30 minutes, the reaction mixture was quenched by NH₄Cl (saturated, aqueous solution) and extracted with EtOAc. The extracts were washed with brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure. The material was purified by column chromatography on silica (Biotage 10 g prepacked column, 0% to 25% EtOAc in hexanes gradient), yielding 0.50 g (72%) of compound S5-1: R_(f) 0.35 (30% EtOAc/hexanes); ¹H NMR (400 MHz, CDCl₃) δ 7.52-7.46 (m, 2H), 7.40-7.32 (m, 5H), 7.28-7.20 (m, 1H), 7.08-7.00 (m, 2H), 5.41 (q, J=6.9 Hz, 1H), 5.09 (s, 2H), 2.34 (s, 1H), 1.63 (d, J=6.9 Hz, 3H); MS (ESI) m/z 481.14, 483.1 (M+Na).

Manganese dioxide (activated, 0.20 g, 2.07 mmol, 5.0 equiv) was added to a solution of compound S5-1 (0.19 g, 0.41 mmol, 1.0 equiv) in dichloromethane (5 m L). After stirring overnight, the reaction was ˜30% complete. Additional manganese dioxide (activated, 0.20 g, 2.07 mmol, 5.0 equiv) was added. After stirring overnight, the reaction mixture was diluted with EtOAc (20 mL) and filtered through Celite. The filtrate was concentrated under reduced pressure, yielding 0.16 g (83%) of compound S5-2: R_(f) 0.24 (10% EtOAc/hexanes); MS (ESI) m/z 479.10, 481.10 (M+Na).

Compound S5-2 (55 mg, 0.12 mmol, 1.0 equiv), piperidine (0.059 mL, 0.60 mmol, 5.0 equiv), and titanium(IV) isopropoxide (0.18 mL, 0.60 mmol, 5.0 equiv) were stirred in dichloromethane (0.20 mL) overnight. Additional piperidine (0.20 mL, 2.00 mmol, 1.7 equiv) and titanium(IV) isopropoxide (0.20 mL, 0.67 mmol, 5.6 equiv) were added, and the reaction mixture was heated to 50° C. overnight. The reaction mixture was diluted with MeOH (2 mL) and NaBH₄ (15 mg, 0.40 mmol, 3.3 equiv) was added. Additional NaBH₄ (10 mg portions, 0.26 mmol, 2.2 equiv) was added every 30 minutes for 4 hrs, resulting in 75 conversion. More MeOH (10 mL) and NaBH₄ (0.10 g, 2.64 mmol, 22 equiv) were added. After bubbling ceased, the reaction mixture was concentrated under reduced pressure. The resulting solids were dissolved in EtOAc, washed with NaHCO₃ (saturated, aqueous solution, 3 times) and brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure, yielding 55 mg (87% crude) of compound S5-3-1. The material was concentrated from toluene (2 times) and used without further purification: R_(f) 0.35 (30% EtOAc/hexanes; MS (ESI) m/z 526.18, 528.17 (M+H).

n-Butyllithium (0.074 mL, 2.5 M/hexanes, 0.18 mmol, 2.25 equiv) was added to diisopropylamine (0.026 mL, 0.18 mmol, 2.25 equiv) in THF (2 mL) at −40° C. The reaction mixture was cooled to −78° C., and TMEDA (0.072 mL, 0.48 mmol, 6.0 equiv) was added. A solution of compound S5-3-1 (55 mg, 0.10 mmol, 1.25 equiv) in THF (1 mL) was added dropwise. The reaction was stirred at −78° C. for 5 min. A solution of enone S1-9 (39 mg, 0.080 mmol, 1.0 equiv) in THF (0.5 mL) was added dropwise to the reaction mixture. Additional LDA (20.050 mL, 2.0 M/heptane/THF/ethylbenzene, 0.10 mmol, 1.25 equiv) was added. The reaction was stirred from −78° C. to −20° C. for 45 minutes, quenched by saturated aqueous NH₄Cl, and extracted with EtOAc (2 times). The combined EtOAc extracts were dried (sodium sulfate) and concentrated to yield the crude product, which was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; gradient: 20→100% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 7.2-8.6 min, were collected and freeze-dried to give 26 mg of compound S5-4-1 (36%): ¹H NMR (400 MHz, CDCl₃) δ 16.0-15.9 (m, 1H), 7.58-7.45 (m, 4H), 7.40-7.28 (m, 6H), 5.35 (s, 2H), 5.03-4.89 (m, 2H), 4.52-4.34 (br s, 1H), 3.92 (d, J=10.4 Hz, 1H), 3.29-3.19 (m, 1H), 3.06-2.93 (m, 1H), 2.78-2.58 (m, 1H), 2.58-2.34 (m, 11H), 2.22-1.76 (br m, 2H), 1.72-1.36 (br m, 9H), 0.88-0.76 (m, 9H), 0.26 (s, 3H), 0.12 (s, 3H); MS (ESI) m/z 914.30, 916.32 (M+H).

A solution of compound S5-4-1 (26 mg, 0.028 mmol) in 1,4-dioxane (1 mL) was treated with HF (0.40 mL, 48-50% aqueous solution). After stirring overnight, the mixture was poured into a solution of K₂HPO₄ (4.8 g) in water (20 mL). This mixture was extracted with EtOAc (3 times). The combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure.

The above material was dissolved in MeOH (2 mL), 1,4-dioxane (2 mL), and 0.5 N HCl/MeOH (0.5 mL). 10% Pd—C (Degussa, 5 mg) was added, and an atmosphere of hydrogen was introduced. After 2 hrs, the reaction mixture was filtered through Celite and concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Polymerx 10μ RP-γ100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: CH₃CN, gradient elution with 0→70% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 6.8-8.2 min, were collected and freeze-dried to yield 12 mg (68%) of compound S5-5-1: ¹H NMR (400 MHz, CD₃OD/DC) δ 7.17 (d, J=5.0 Hz, 1H), 4.80-4.68 (m, 1H), 4.15 (s, 1H), 3.80-3.72 (m, 1H), 3.42-3.30 (m, 1H), 3.26-2.78 (m, 11H), 2.40-2.25 (m, 2H), 2.05-1.71 (m, 8H), 1.70-1.40 (m, 2H); MS (ESI) m/z 544.15 (M+H).

Example 6. Synthesis of Compounds Via Scheme 6

The following compounds were prepared according to Scheme 6.

Compound S3-1 (0.36 g, 0.81 mmol, 1.0 equiv) was dissolved in 1,2-dichloroethane (15 mL) with triethylamine (0.57 mL, 4.07 mmol, 5.0 equiv). Methanesulfonic anhydride (0.71 g, 4.07 mmol, 5.0 equiv) was added in one portion at 0° C. During the addition, color change was observed. After stirring at rt for one hour, LC/MS indicated that the starting material was consumed. The reaction mixture was diluted with dichloromethane, quenched by pH 7 phosphate buffer, washed with water (2×20 mL) and brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure. The crude intermediate was used without further purification.

The above crude intermediate (0.16 g, 0.30 mmol, 1.0 equiv) was dissolved in 1,2-dichloroethane (2 mL) with diisopropylethylamine (0.13 mL, 0.72 mmol, 2.4 equiv). 2,2-Dimethyl piperidine hydrochloric salt (54 mg, 0.36 mmol, 1.2 equiv) was first neutralized with sodium hydroxide solution and then was added to reaction mixture in one portion at rt. After stirring at 60° C. for four days, LC/MS indicated that most of the starting material was consumed. The reaction mixture was diluted with dichloromethane, washed with water (2×20 mL) and brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure to afford the desired product S6-2-1, which was used without further purification.

A solution of LDA was prepared by adding n-BuLi (0.31 mL, 1.6 M/hexanes, 0.50 mmol, 2.5 equiv) to diisopropylamine (71 μL, 0.50 mmol, 2.5 equiv) in 2 mL dry THF under a nitrogen atmosphere in a flame dried schenck flask at −78° C. The pale solution was warmed to −20° C., stirred for 15 min, and cooled down to −78° C. TMEDA (75 μL, 0.50 mmol, 2.5 equiv) was added slowly via a syringe, followed by the dropwise addition of compound S6-2-1 (crude, 0.30 mmol, 1.5 equiv)/THF (1 mL). A dark-red color appeared as soon as addition started. After stirring for 10 min, enone S1-9 (96 mg, 0.20 mmol, 1.0 equiv) in 1 mL dry THE was added slowly via a syringe. After 10 min, LC/MS indicated that the enone was consumed and the product present. The reaction mixture was allowed to slowly warm to −20° C. in 1 h. Phosphate buffer (pH 7, 10 mL) and saturated aqueous ammonium chloride (20 mL) were added. The resulting mixture was extracted with dichloromethane (3×15 mL), and the combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure to yield crude S6-3-1 as a red-orange oil (97 mg).

Aqueous HF (0.3 mL, 48-50%) was added to a CH₃CN solution (1.0 mL) of S6-3-1 (97 mg) in a plastic vial at 25° C. The reaction was stirred at 25° C. for 18 hrs. The reaction mixture was poured into an aqueous solution (10 mL) of K₂HPO₄ (2 g). The solution was extracted with EtOAc (3×15 mL). The combined EtOAc extracts were dried over sodium sulfate and concentrated to give the crude intermediate (18 mg).

Pd—C (5 mg, 10 wt %) was added to a MeOH solution (2 mL) of the above crude intermediate. HCl in MeOH (0.5 N, 0.5 mL) was added. The reaction was stirred under H₂ (balloon) at 25° C. for 2 hrs and filtered through a pad of Celite. The filtrate was concentrated, and the crude product was purified by HPLC on a Polymerx 10μ. RP-γ100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: CH₃CN, sample in 2.0 mL (0.05 N HCl), gradient elution with 0→70% B over 15 min, mass-directed fraction collection]. Fractions containing the desired product were collected and freeze-dried, yielding 9 mg of the desired product S6-4-1 as a yellow solid (5%, five steps): ¹H NMR (400 MHz, CD₃OD) δ 7.04 (d, J=5.5 Hz, 1H), 4.75 (dd, J=13.7, 7.3 Hz, 1H), 4.08 (s, 1H), 3.92 (dd, J=13.7, 7.3 Hz, 1H), 3.32 (m, 2H), 3.24-2.98 (m, 3H), 3.03 (s, 3H), 2.96 (s, 3H), 2.37 (t, J=15.1 Hz, 1H), 2.27-2.20 (m, 1H), 1.91-1.82 (m, 2H), 1.80-1.63 (m, 51H), 1.63 (s, 3H), 1.50 (s, 3H); MS (ESI) in m/z 558.30 (M+H).

The following compounds were prepared similarly to S6-4-1.

¹H NMR (400 MHz, CD₃OD) δ 9.11 (s, 1H), 7.68 (m, 1H), 7.62 (m, 1H), 6.86 (d, J=5.9 Hz, 1H), 5.54 (s, 2H), 4.07 (s, 1H), 3.22-2.93 (m, 9H), 2.37-2.27 (m, 1H), 2.24-2.17 (m, 1H) 1.70-1.57 (m, 1H); MS (ESI) m/z 513.17 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.06 (dd, J=8.2, 6.0 Hz, 1H), 4.39 (s, 2H), 4.10 (s, 1H), 3.65-3.54 (m, 2H), 3.24-92 (m, 9H), 2.43-2.32 (m, 1H), 2.28-2.17 (m, 1H), 2.07-1.58 (m, 7H), 1.51 (d, J=6.0 Hz, 3H), 1.39 (d, J=6.0 Hz, 3H); MS (ESI) m/z 558.61 (M+H).

Example 7. Synthesis of Compounds Via Scheme 7

The following compounds were prepared according to Scheme 7.

To a solution of sodium bicarbonate (0.21 g, 2.48 mmol, 1.1 equiv) in water (10 mL) was added O-benzylhydroxylamine hydrochloride (0.40 g, 2.48 mmol, 1.1 equiv). The solution was heated until all solid dissolved. Compound S1-7 (1.00 g, 2.26 mmol, 1.0 equiv) in EtOH (6 mL) and 1,4-dioxane (6 mL) were added. The solution was heated until all solid dissolved, and then stirred at rt for 2 hrs. The mixture was diluted with EtOAc (100 mL), washed with brine (20 mL×3), dried (sodium sulfate), and concentrated. The residue was purified by flash chromatography on silica gel, eluting with hexanes/EtOAc (30:1 to 5:1) to afford 1.10 g (89%) of compound S7-1: ¹H NMR (400 MHz, CDCl₃) δ 8.33 (s, 1H), 7.48-7.24 (comp, 13H), 7.03-6.96 (comp, 2H), 5.22 (s, 2H), 5.03 (s, 2H), 2.30 (d, J:=2.4 Hz, 3H); MS (ESI) m/z 548.16 (M+H).

To a solution of LDA (0.38 mL, 1.0 M/THF, 0.38 mmol, 2.9 equiv) in THF (1 mL) was added TMEDA (57 μL, 0.38 mmol, 2.9 equiv) at −78° C. After stirring for 5 min, a solution of compound S7-1 (89 mg, 0.16 mmol, 1.2 equiv) in THF (0.3 mL) was added dropwise to the LDA solution. After complete addition, the reaction mixture was stirred at −78° C. for 10 min. A solution of enone S1-9 (60 mg, 0.13 mmol, 1.0 equiv) in THF (0.3 mL) was added dropwise. The mixture was slowly warmed to −20° C. over 30 min. The mixture was quenched by phosphate buffer (pH 8, 1.5 mL) and saturated ammonium chloride solution (0.5 mL). The aqueous layer was extracted with EtOAc (3 mL×4). All organic layers were combined, dried (sodium sulfate), and concentrated. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 2.0 mL. (CH₃CN); gradient: 80→100% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 6.1-8.3 min, were collected and freeze-dried to give 33 mg of S7-2 (32%): ¹H NMR (400 MHz, CDCl₃) δ 15.71 (s, 1H), 7.55-7.24 (comp, 10H), 5.35 (s, 2H), 4.99 (d, J=9.8 Hz, 1H), 4.95 (d, J=9.8 Hz, 1H), 3.87 (d, J=10.4 Hz, 1H), 3.24 (dd, J=16.5, 4.6 Hz, 1H), 3.07-2.97 (m, 1H), 2.49 (s, 6H), 2.64-2.33 (comp, 3H), 2.20-2.12 (m, 1H), 0.80 (s, 9H), 0.26 (s, 3H), 0.12 (s, 3H); MS (ESI) m/z 828.16 (M+H).

To a solution of S7-2 (33 mg, 0.040 mmol) in acetonitrile (1 mL) was added HF (0.3 mL, 48-50% solution in water). The mixture was stirred at 0° C. for 63 hrs and rt for 3 hrs. The mixture was quenched by potassium phosphate dibasic solution (prepared from 8 g K₂HPO₄ and 8 mL water). The aqueous layer was extracted with EtOAc (8 mL×5). All organic layers were combined, dried (sodium sulfate) and concentrated to afford the crude intermediate.

To the above crude intermediate in MeOH/dioxane solution (1:1, 1.4 mL) was added Pd—C (15 mg, 10 wt %) and HCl/MeOH (0.5 N, 0.25 mL). The reaction was bubbled with H₂ (balloon) at 25° C. for 90 min. The mixture was filtered through a small Celite plug and flashed with MeOH. The filtrate was concentrated to yield the crude product, which was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-□ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 4.5 mL (0.05 N HCl/water); gradient: 0→50% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 6.9-8.1 min, were collected and freeze-dried to yield 10 mg of primary S7-3 (55% for 2 steps): ¹H NMR (400 MHz, CD₃OD) δ 6.95 (d, J=6.1 Hz, 1H), 4.18 (s, 2H), 4.09 (s, 1H), 3.04 (s, 3H), 2.96 (s, 3H), 3.22-2.90 (comp, 3H), 2.39-2.28 (m, 1H), 2.28-2.18 (m, 1H), 1.70-1.57 (m, 1H); MS (ESI) m/z 462.12 (M+H).

To a solution of compound S7-3 (10 mg, 0.020 mmol, 1.0 equiv) and Ac₂O (diluted 20 times in dichloromethane, 45 μL, 0.020 mmol, 1.0 equiv) in DMF (1.5 mL) was added Et₃N (15 μL, 0.11 mmol, 5.5 equiv) at 0° C. The mixture was stirred for 30 min and then submitted to preparative reverse phase HPLC purification on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HC/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 0→70% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 9.4-10.6 min, were collected and freeze-dried to yield 4 mg of S7-4-1 (40%): ¹H NMR (400 MHz, CD₃OD) δ 6.73 (d, J=5.5 Hz, 1H), 4.38 (d, J=1.8 Hz, 2H), 4.07 (s, 1H), 3.15 (dd, J=15.3, 4.3 Hz, 1H), 3.03 (s, 3H), 2.95 (s, 3H), 3.10-2.92 (comp, 2H), 2.32-2.23 (m, 1H), 2.22-2.15 (m, 1H), 2.01 (s, 3H), 1.68-1.56 (m, 1H); MS (ESI) m/z 504.10 (M+H).

S7-4-2 was prepared according to the procedure for the preparation of S7-4-1: ¹H NMR (400 MHz, CD₃OD) δ 6.65 (d, J=6.1 Hz, 1H), 4.41 (d, J=16.5 Hz, 1H), 4.35 (d, J=16.5 Hz, 1H), 4.06 (s, 1H), 3.15 (dd, J=15.3, 4.6 Hz, 1H), 3.03 (s, 3H), 2.95 (s, 3H), 3.10-2.91 (comp, 2H), 2.33-2.22 (m, 1H), 2.22-2.14 (m, 1H), 1.68-1.56 (m, 1H), 1.22 (s, 9H); MS (ESI) m/z 546.27 (M+H).

To a solution of compound S7-3 (11 mg, 0.020 mmol, 1.0 equiv) and methyl isocyanate (1.4 mg, 0.020 mmol, 1.0 equiv) in DMF (1.5 mL) was added Et₃N (18 μL, 0.13 mmol, 6.5 equiv) at 0° C. The mixture was stirred for 10 min and then submitted to preparative reverse phase HPLC purification on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.3 mL (0.05 N HCl/water); gradient: 0→70% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 9.3-10.6 min, were collected and freeze-dried to yield 4 mg of S7-4-3 (31%): ¹H NMR (400 MHz, CD₃OD) δ 6.74 (d, J=6.1 Hz, 1H), 4.37 (d, J=17.3 Hz, 1H), 4.32 (d, J=17.3 Hz, 1H), 4.06 (s, 1H), 3.15 (dd, J=15.9, 4.6 Hz, 1H), 3.03 (s, 3H), 2.95 (s, 3H), 3.10-2.92 (comp, 2H), 2.72 (s, 3H), 2.32-2.22 (m, 1H), 2.22-2.15 (m, 1H), 1.67-1.56 (m, 1H); MS (ESI) m/z 519.08 (M+H).

S7-4-4 was prepared from S7-3 according to the procedure for the preparation of S7-4-1: ¹H NMR (400 MHz, CD₃OD) δ 6.79 (d, J=6.1 Hz, 1H), 4.49 (d, J=5.5 Hz, 2H), 4.07 (s, 1H), 4.03 (s, 2H), 3.15 (dd, J=15.2, 4.3 Hz, 1H), 3.03 (s, 3H), 2.95 (s, 3H), 2.94 (s, 6H), 3.10-2.92 (comp, 2H), 2.33-2.23 (m, 1H), 2.22-2.17 (m, 1H), 1.68-1.56 (m, 1H); MS (ESI) m/z 547.11 (M+H).

To a solution of compound S7-3 (11 mg, 0.020 mmol, 1.0 equiv) and methanesulfonic anhydride (5 mg, 0.030 mmol, 1.5 equiv) in DMF (1.5 mL) was added Et₃N (18 μL, 0.13 mmol, 6.5 equiv) at 0° C. The mixture was stirred for 50 min and then submitted to preparative reverse phase HPLC purification on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3 mL (0.05 N HCl/water); gradient: 0→70% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 10.3-11.6 min, were collected and freeze-dried to yield 3 mg of S7-4-5 (22%): ¹H NMR (400 MHz, CD₃OD) δ 6.93 (d, J=6.1 Hz, 1H), 4.29 (s, 2H), 4.06 (s, 1H), 3.16 (dd, J=15.9, 4.6 Hz, 1H), 3.03 (s, 3H), 2.94 (s, 3H), 2.93 (s, 3H), 3.10-2.92 (comp, 2H), 2.34-2.23 (m, 1H), 2.23-2.16 (m, 1H), 1.68-1.57 (m, 1H); MS (ESI) m/z 540.04 (M+H).

S7-4-6 was prepared according to the procedure for the preparation of S7-4-5: ¹H NMR (400 MHz, CD₃OD) δ 7.79 (d, J=7.4 Hz, 2H), 7.59-7.48 (comp, 3H), 6.76 (d, J=6.1 Hz, 1H), 4.12 (s, 2H), 4.06 (s, 1H), 3.03 (s, 3H), 2.95 (s, 3H), 3.15-2.91 (comp, 3H), 2.24-2.13 (comp, 2H), 1.67-1.54 (m, 1H); MS (ESI),/z 602.22 (M+H).

The following compounds were prepared from S2-4-17 using similar amide, urea, or sulfonamide formation conditions.

To a solution of S2-4-17 (8 mg, 0.020 mmol, 1.0 equiv) and Ac₂O) (diluted 20 times in dichloromethane, 38 μL, 0.020 mmol, 1.0 equiv) in DMF/acetonitrile (1:1, 2 mL) was added Et₃N (9 μL, 0.070 mmol, 3.5 equiv) at rt. The mixture was stirred for 30 min and then submitted to preparative reverse phase HPLC purification on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 1.5 mL (0.05 N HCl/water); gradient: 0→100% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 8.1-9.0 min, were collected and freeze-dried to yield 5 mg of S7-4-7 (52%): ¹H NMR (400 MHz, CD₃OD) δ 6.62 (d, J=6.1 Hz, 1H), 4.67-4.60 (m, 2H), 4.07 (s, 1H), 3.09 (s, 3H), 3.03 (s, 3H), 2.95 (s, 3H), 3.21-2.90 (comp, 3H), 2.36-2.12 (comp, 5H), 1.68-1.58 (m, 1H); MS (ESI) m/z 518.12 (M+H).

S7-4-8:

¹H NMR (400 MHz, CD₃OD) δ 6.64-6.56 (m, 1H), 4.63 (br s, 2H), 4.07 (s, 1H), 3.25 (s, 3H), 3.03 (s, 3H), 2.95 (s, 3H), 3.21-2.92 (comp, 3H), 2.35-2.16 (comp, 2H), 2.07-1.99 (m, 1H), 1.70-1.57 (m, 1H), 0.94-0.76 (comp, 4H); MS (ESI) m/z 544.25 (M+H).

S7-4-9:

¹H NMR (400 MHz, CD₃OD) δ 6.53 (d, J=6.1 Hz, 1H), 4.63 (d, J=4.3 Hz, 2H), 4.06 (s, 1H), 3.17 (s, 3H), 3.03 (s, 3H), 2.95 (s, 3H), 3.20-2.92 (comp, 3H), 2.37-2.16 (comp, 2H), 1.68-1.58 (m, 1H), 1.32 (s, 9H); MS (ESI) m/z 560.30 (M+H).

To a solution of S2-4-17 (15 mg, 0.030 mmol, 1.0 equiv) and methyl isocyanate (2 mg, 0.030 mmol, 1.0 equiv) in DMF (1.5 mL) was added Et₃N (22 μL, 0.16 mmol, 5.3 equiv) at 0° C. The mixture was stirred for 30 min and then submitted to preparative reverse phase HPLC purification on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 2.6 mL (0.05 N HCl/water); gradient: 0→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 8.4-10.2 min, were collected and freeze-dried to yield 14 mg of S7-4-10 (83%): ¹H NMR (400 MHz, CD₃OD) δ6.59 (d, J=6.1 Hz, 1H), 4.55 (s, 2H), 4.07 (s, 1H), 3.03 (s, 3H), 2.95 (s, 3H), 2.91 (s, 3H), 3.19-2.89 (comp, 3H), 2.75 (s, 3H), 2.32-2.16 (comp, 2H), 1.68-1.57 (m, 1H); MS (ESI) m/z 533.11 (M+H).

To a solution of S2-4-17 (15 mg, 0.030 mmol, 1.0 equiv) and methanesulfonic anhydride (6 mg, 0.030 mmol, 1.0 equiv) in DMF (1.5 mL) was added Et₃N (22 μL, 0.16 mmol, 5.3 equiv) at 0° C. The mixture was stirred at 0° C. for 30 min and rt for 20 min. The mixture was submitted to preparative reverse phase HPLC purification on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3 mL (0.05 N HCl/water); gradient: 0→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 9.8-11.6 min, were collected and freeze-dried to yield 7 mg of S7-4-11 (38%): ¹H NMR (400 MHz, CD₃OD) δ 6.90 (d, J=5.5 Hz, 1H), 4.38 (s, 2H), 4.07 (s, 1H), 3.03 (s, 3H), 2.95 (s, 3H), 2.94 (s, 3H), 3.19-2.93 (comp, 3H), 2.83 (s, 3H), 2.34-2.16 (comp, 2H), 1.69-1.58 (m, 1H); MS (ESI) m/z 554.06 (M+H).

S7-4-12 was prepared according to the procedure for the preparation of S7-4-11: ¹H NMR (400 MHz, CD₃OD) δ 7.85 (dd, J=7.4, 1.8 Hz, 2H), 7.72-7.60 (comp, 3H), 6.87 (d, J=6.1 Hz, 1H), 4.25 (d, J=1.8 Hz, 2H), 4.06 (s, 1H), 3.03 (s, 3H), 2.95 (s, 3H), 3.15-2.92 (comp, 3H), 2.71 (s, 3H), 2.32-2.16 (comp, 2H), 1.68-1.57 (m, 1H); MS (ESI) m/z 616.20 (M+H).

Example 8. Synthesis of Compounds Via Scheme 8

The following compounds were prepared according to Scheme 8.

(Methoxymethyl)triphenylphosphonium chloride (0.46 g, 1.35 mmol, 2.0 equiv) was added to a suspension of potassium t-butoxide (0.15 g, 1.35 mmol, 2.0 equiv) in THF (3 mL), resulting in a red colored mixture. After 15 minutes, a solution of compound S1-7 (0.30 g, 0.68 mmol, 1.0 equiv) in THF (2 mL) was added, resulting in a yellowish orange mixture. After 1 h, the reaction mixture was quenched by water (15 mL) and extracted with EtOAc (2×20 mL). The combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure. The material was purified by column chromatography on silica (Biotage 10 g prepacked column, 0% to 6% EtOAc in hexanes gradient). The two regioisomeric compounds mostly co-eluted and were combined, yielding 0.23 g (73%) of compound S8-1 (¹H NMR indicated a 2:1 mixture of cis and trans isomers): R_(f) 0.40, 0.34 (10% EtOAc/hexanes); ¹H NMR (400 MHz, CDCl₃) δ 7.52-7.46 (m, 2H), 7.40-7.32 (m, 5.66H), 7.28-7.20 (m, 1H), 7.04 (d, J=8.2 Hz, 2H), 6.31 (d, J=6.8 Hz, 0.34H), 5.93 (d, J=12.8 Hz, 0.66H), 5.22 (d, J=6.8 Hz, 0.34H), 5.08 (s, 2H), 3.77 (s, 2H), 3.73 (s, 1H), 2.36-2.31 (m, 3H); MS (ESI) m/z 493.26, 495.26 (M+Na).

A solution of S8-1 (0.35 g, 0.74 mmol) in 6 N HCl in water (2 mL) and THF (6 mL) was heated to 70° C. After 4 hrs, the reaction mixture was cooled to room temperature and diluted with EtOAc (25 mL). The layers were separated, and the EtOAc layer was dried over sodium sulfate, filtered, and concentrated under reduced pressure, yielding 0.32 g (95%) of crude aldehyde intermediate: MS (ESI) m/z 479.19, 481.15 (M+Na).

The aldehyde intermediate (0.10 g, 0.13 mmol, 1.0 equiv) was dissolved in 1,2-dichloroethane (2 mL) and HOAc (0.054 mL, 0.94 mmol, 7.2 equiv) and t-butylamine (0.099 mL, 0.94 mmol, 7.2 equiv) were added. After 15 minutes, Na(OAc)₃BH (0.14 g, 0.67 mmol, 5.2 equiv) was added. After stirring overnight, the reaction mixture was quenched by NaHCO₃ (saturated, aqueous solution), diluted with dichloromethane (20 mL), washed with NaHCO₃ (saturated, aqueous solution, 10 mL), dried over sodium sulfate, filtered, and concentrated under reduced pressure. The material was purified by column chromatography on silica (Biotage 5 g prepacked column, 0% to 60% EtOAc in hexanes gradient), yielding 20 mg (28%) of compound S8-2-1: ¹H NMR (400 MHz, CDCl₃) δ 7.52-7.46 (m, 2H), 7.40-7.32 (m, 5H), 7.28-7.20 (m, 1H), 7.04 (d, J=8.2 Hz, 2H), 5.10 (s, 2H), 3.25-2.98 (m, 2H), 2.90-2.70 (m, 2H), 2.35 (s, 3H), 1.17 (br s, 9H); MS (ESI) m/z 514.31, 516.3 (M+H).

A solution of S8-2-1 (20 mg, 0.038 mmol, 1.2 equiv) in THF (1 mL) was added to a solution of LDA (1.2 M/THF/heptane/ethylbenzene, 0.058 mL, 0.070 mmol, 2.2 equiv) and TMEDA (0.028 mL, 0.19 mmol, 6.0 equiv) in THF (2 mL) at −78° C., giving a red colored solution. The reaction was stirred at −78° C. for 5 min. A solution of enone S1-9 (15 mg, 0.032 mmol, 1.0 equiv) in THF (0.5 mL) was added dropwise to the reaction mixture. The reaction was stirred from −78° C. to −20° C. for 1 h, quenched by saturated aqueous NH₄Cl, and extracted with EtOAc (2 times). The combined EtOAc extracts were dried (sodium sulfate) and concentrated to yield the crude product. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; gradient: 20→100% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 7.0-8.0 min, were collected and freeze-dried to give 18 mg of pure compound S8-3-1 (62%): ¹H NMR (400 MHz, CDCl₃) δ 7.58-7.45 (m, 4H), 7.40-7.28 (m, 6H), 5.35 (s, 2H), 5.00-4.87 (m, 2H), 3.91 (d, J=10.4 Hz, 1H), 3.54-3.15 (m, 3H), 3.02-2.88 (m, 3H), 2.58-2.32 (m, 9H), 2.17-2.08 (m, 1H), 1.48-1.20 (br s, 9H), 0.80 (s, 9H), 0.26 (s, 3H), 0.11 (s, 3H); MS (ESI) m/z 902.48, 904.45 (M+H).

A solution of compound S8-3-1 (18 mg, 0.020 mmol) in 1,4-dioxane (0.80 mL) was treated with HF (0.40 mL, 48-50% aqueous solution). After stirring overnight, the mixture was poured into a solution of K₂HPO₄ (4.8 g) in water (20 mL). This mixture was extracted with EtOAc (3 times), and the combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure.

The above material was dissolved in MeOH (1 mL), 1,4-dioxane (1 mL), and 0.5 N HCl/MeOH (0.4 mL). 10% Pd—C (Degussa, 2 mg) was added, and an atmosphere of hydrogen was introduced. After 2 hrs, the reaction mixture was filtered through Celite and concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: CH₃CN, gradient elution with 0→70% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 7.0-8.4 min, were collected and freeze-dried to yield 8 mg of compound S8-4-1 (64%): ¹H NMR (400 MHz, CD OD/DCl) δ 6.86 (d, J=5.5 Hz, 1H), 4.14 (s, 1H), 3.34-2.94 (m, 13H), 2.33-2.21 (m, 2H), 1.70-1.55 (m, 1H), 1.49 (s, 9H); MS (ESI) m/z 532.31 (M+H).

Example 9. Synthesis of Compounds Via Scheme 9

The following compounds were prepared according to Scheme 9.

Compound S1-7 (0.25 g, 0.56 mmol, 1.0 equiv) was dissolved in 1,2-dichloroethane (4 mL). Dimethylamine (0.56 mL, 2.0 M/THF, 1.12 mmol, 2.0 equiv) and acetic acid (64 μL, 1.12 mmol, 2.0 equiv) were added under a nitrogen atmosphere. After stirring at rt for 1 h, sodium triacetoxyborohydride (0.36 g, 1.68 mmol, 3.0 equiv) was added to reaction mixture. After overnight, LC/MS indicated that the starting material was consumed. The reaction mixture was diluted with dichloromethane, washed with NaHCO₃ (saturated aqueous solution, 3×10 mL) and brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure. The crude product was purified by flash column chromatography (Biotage 10 g column, 10% to 30% EtOAc in hexanes gradient), yielding 0.21 g (78%) of the pure compound S9-1-1 as a colorless oil: ¹H NMR (400 MHz, CDCl₃) δ 7.52-7.47 (m, 2H), 7.40-7.33 (m, 5H), 7.26 (t, J=7.8 Hz, 1H), 7.07-7.03 (m, 2H), 5.11 (s, 2H), 3.66 (d, J=2.3 Hz, 2H), 2.37 (d, J=2.3 Hz, 3H), 2.35 (s, 6H).

Compound S9-1-1 (0.10 g, 0.21 mmol), methylboronic acid (38 mg, 0.64 mmol), dichlorobis(tricyclohexylphosphine)palladium(II) (4 mg, 0.011 mmol) and K₃PO₄ (0.14 g, 0.64 mmol) were heated to 80° C. in toluene (2 mL) and water (5 drops). After 5 hrs, additional K₃PO₄ (0.14 g, 0.64 mmol) and methylboronic acid (38 mg, 0.64 mmol) were added. The reaction mixture was heated to 100° C. After one hour, the reaction mixture was cooled to rt and let stand for 3 days. Additional dichlorobis-(tricyclohexylphosphine)palladium(II) (5 mg, 0.015 mmol) and methylboronic acid (38 mg, 0.64 mmol) were added, followed by enough K₃PO₄ to give a saturated aqueous layer. This was heated to 110° C. After 2 hrs, the reaction was complete. Upon cooling to rt, the reaction mixture was diluted with EtOAc, washed with water (2 times) and brine (1 time), dried over sodium sulfate, filtered, and concentrated under reduced pressure. The material was purified by column chromatography on silica (Biotage 5 g prepacked column, 0% to 6% MeOH in dichloromethane gradient), yielding 81 mg (93%) of compound S9-2-1. The compound was ˜80-90% pure and contaminated with phosphine ligand: R_(f) 0.49 (10% MeOH/dichloromethane); ¹H NMR (400 MHz, CDCl₃) δ 7.50-7.30 (m, 7H), 7.30-7.20 (m, 1H), 7.12-7.04 (m, 2H), 4.96 (s, 2H), 2.80-2.20 (m, 9H), 1.56 (s, 3H); MS (ESI) m/z 408.34 (M+H).

n-Butyllithium (2.5 M/hexanes, 0.14 mL, 0.35 mmol, 2.3 equiv) was added to a −40° C. solution of diisopropylamine (0.050 mL, 0.35 mmol, 2.3 equiv) in THF (5 mL). The reaction mixture was cooled to −78° C., and TMEDA (0.14 mL, 0.92 mmol, 6.1 equiv) was added. A solution of S9-2-1 (81 mg, 0.20 mmol, 1.3 equiv) in THF (2 mL) was added dropwise. A solution of enone S1-9 (74 mg, 0.15 mmol, 1.0 equiv) in THF (1 mL) was added dropwise to the reaction mixture. The reaction was stirred from −78° C. to −20° C. for 1 h, quenched by saturated aqueous NH₄Cl, and extracted with EtOAc (2 times). The combined EtOAc extracts were dried (sodium sulfate) and concentrated to yield the crude product. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; gradient: 50→100% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 2.5-3.6 min, were collected and freeze-dried to give 34 mg of compound S9-3-1 (28%, contaminated with the phosphine ligand): MS (ESI) m/z 796.53 (M+H).

A solution of compound S9-3-1 (34 mg, 0.043 mmol) in 1,4-dioxane (0.9 mL) was treated with HF (0.40 mL, 48-50% aqueous solution). After stirring overnight, the mixture was poured into a solution of K₂HPO₄ (4.8 g) in water (20 mL). This mixture was extracted with EtOAc (3 times), and the combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure.

The above crude material was dissolved in MeOH (2 mL), 1,4-dioxane (2 mL), and 0.5 N HCl/MeOH (0.5 mL). 10% Pd—C (Degussa, 10 mg) was added, and an atmosphere of hydrogen was introduced. Upon completion, the reaction mixture was filtered through Celite and concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: CH₃CN, gradient elution with 0→70% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 6.6-7.6 min, were collected and freeze-dried to yield 10 mg of compound S9-4-1 (41%): ¹H NMR (400 MHz, CD₃OD/DCl) δ 4.52 (s, 2H), 4.15 (s, 1H), 3.24-2.92 (m, 15H), 2.36 (s, 3H), 2.34-2.24 (m, 2H), 1.70-1.56 (m, 1H); MS (ESI) m/z 504 (M+H).

Example 10. Synthesis of Compounds Via Scheme 10

The following compounds were prepared according to Scheme 10.

To a 250 mL round bottom flask was added compound S1-4 (14.47 g, 56.30 mmol, 1.0 equiv, crude), tetrabutylammonium bromide (0.90 g, 2.80 mmol, 0.05 equiv), 1,2-dichloroethane (60 mL), and water (60 mL). The clear bi-layer was cooled in a 20° C. water bath. Nitric acid (7.2 mL, 70 wt %, 112.60 mmol, 2.0 equiv) was added. After the addition, the reaction temperature slowly rose to 26° C. The reaction was stirred at room temperature overnight (19 hrs). TLC (heptane/EtOAc=9.5/0.5) showed the reaction was complete. The organic layer was separated, washed with water (60 mL×2) and brine, and dried over anhydrous sodium sulfate. The solvent was removed to give compound S10-1 as a brown oil, which solidified on standing (17.71 g, quantitative). The crude product was used directly for the next step.

To a 250 mL round bottom flask was added compound S10-1 (17.7 g, 56.30 mmol 1.0 equiv), acetone (177 mL), anhydrous potassium carbonate (15.6 g, 113.00 mmol, 2.0 equiv), and potassium iodide (0.47 g, 2.80 mmol, 0.05 equiv). To the stirred suspension at room temperature was added benzyl bromide (7.03 mL, 59.10 mmol, 1.05 equiv). The suspension was then heated to 56° C. for 4 hrs. TLC (heptane/EtOAc=9/1) showed the reaction was complete. The solid was removed by filtration and washed with acetone (30 mL). The filtrated was concentrated to give a paste. The paste was partitioned between methyl t-butyl ether (MTBE, 120 mL) and water (80 mL). The organic layer was washed with water (80 mL) and brine, dried over anhydrous sodium sulfate, and concentrated to give compound S10-2 as a brown oil (21.09 g, 98%). The crude product was used directly for the next step.

To a 1 L round bottom flask was added compound S10-2 (21.08 g, 5540 mmol, 1.0 equiv) and THF (230 mL). The solution was cooled in a cold water bath to 10° C. To another 500 mL round bottom flask containing water (230 mL), sodium hydrosulfite (Na₂S₂O₄, 56.7 g, 276.80 mmol, 5.0 equiv) was added slowly with stirring. The aqueous solution of sodium hydrosulfite was added to the THF solution of compound S10-2. The temperature quickly rose from 10° C. to 20.4° C. after the addition. The yellow suspension was stirred while the cold water bath slowly warmed up to room temperature overnight to give an orange cloudy solution. The reaction temperature during this period was between 15° C. to 19° C. TLC (heptane/EtOAc=9/1) showed the reaction was complete. The orange cloudy solution was diluted with EtOAc (460 mL). The organic layer was washed with water (150 mL×2) and brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure to give the crude product as a brown oil. The crude product was purified by flash silica gel column eluted with heptane/EtOAc 9/1 to yield the desired product S10-3 (15.83 g, 80%, 3 steps).

Di-t-butyl dicarbonate (10.56 g, 48.40 mmol, 2.5 equiv) and DMAP (0.12 g, 0.97 mmol, 0.05 equiv) were added to the solution of S10-3 (6.80 g, 19.40 mmol, 1.0 equiv) in anhydrous DMF (39 mL). The resulting mixture was stirred at rt for 20 hrs and diluted with EtOAc. The solution was washed with H₂O (three times) and brine, dried over sodium sulfate, filtered and concentrated. Further purification of the residue by flash chromatography (silica gel, 95:5 hexanes/EtOAc) yielded compound S10-4 as a white solid (8.70 g, 81%): ¹H NMR (400 MHz, CDCl₃) δ 1.39 (s, 18H), 2.36 (d, J=1.8 Hz, 3H), 4.92 (s, 2H), 6.96-7.02 (m, 3H), 7.22-7.38 (m, 8H); MS (ESI) m/z 574.3 (M+Na), calcd for C₃₁H₃₄FNO₃Na 574.2.

To compound S10-4 (4.80 g, 8.70 mmol, 1.0 equiv) in anhydrous THF (50 mL) at −78° C. was added LDA (7.25 mL, 1.8 M/heptane/ethylbenzene/THF, 13.05 mmol, 1.5 equiv) dropwise over a period of 2 min. The resulting deep red-brown solution was stirred at −78° C. for 30 min. Anhydrous DMF (1.35 mL, 17.44 mmol, 2.0 equiv) was added. The resulting light brown solution was stirred at −78° C. for 30 min. The reaction was quenched at −78° C. with HOAc (0.90 mL), warmed up to rt, diluted with EtOAc (200 mL), washed with water (500 mL×1), saturated aqueous sodium bicarbonate (100 mL×1), and brine (100 mL×1). The EtOAc solution was dried over sodium sulfate and concentrated under reduced pressure. Flash column chromatography on silica gel with 0%-15% EtOAc/hexanes yielded the desired product S10-5 as an orange solid (2.55 g, 61%): R_(f) 0.50 (20% EtOAc/hexanes): ¹H NMR (400 MHz, CDCl₃) δ 10.26 (s, 1H), 7.39 (br s, 1H), 7.30-7.42 (m, 7H), 7.23-7.30 (m, 1H), 7.07 (d, J=7.3 Hz, 2H), 4.96 (s, 2H), 2.36 (d, J=2.4 Hz, 3H), 1.44 (s, 9H); MS (ESI) m/z 480.1 (M+H), calcd for C₂₇H₂₆FNO₆ 479.2.

To a solution of S10-5 (0.40 g, 0.83 mmol, 1.0 equiv) in 1,2-dichloroethane (8.4 mL) was added dimethylamine (33% in EtOH, 0.57 mL, 4.18 mmol, 5.0 equiv) and acetic acid (0.14 mL, 2.50 mmol, 3.0 equiv). The mixture was stirred at rt for 2 hrs. Na(OAc)₃BH (0.53 g, 2.50 mmol, 3.0 equiv) was added, and the reaction was stirred for 15 hrs. The mixture was diluted with EtOAc (15 mL). The organic layer was washed two times with potassium phosphate dibasic solution (prepared from 2 g K₂HPO₄ and 5 mL water), dried (sodium sulfate) and concentrated.

To the above residue in DMF (8 mL) was added Boc₂O (0.24 g, 1.08 mmol, 1.3 equiv), DMAP (10 mg, 0.080 mmol, 0.1 equiv) and Et₃N (0.58 mL, 4.20 mmol, 5.1 equiv). The reaction was stirred at rt for 2 hrs. Sodium hydride (60% dispersion in mineral oil, 0.18 g, 4.40 mmol, 5.3 equiv) was added, and the mixture was stirred for 2 hrs. The mixture was quenched by brine (2 mL) and diluted with EtOAc (100 mL). The organic layer was washed with brine (30 mL×5), dried (sodium sulfate) and concentrated. The residue was purified by flash chromatography on silica gel, eluting with hexanes/EtOAc (5:1) to afford 0.52 g of compound 60 (quant. yield): ¹H NMR (400 MHz, CDCl₃) δ 7.40-7.18 (comp, 8H), 7.02-6.96 (comp, 2H), 4.96 (s, 2H), 3.37 (s, 2H), 2.37 (d, J=2.4 Hz, 3H), 2.22 (s, 6H), 1.36 (s, 18H); MS (ESI) m/z 609.33 (M+H).

To a solution of i-Pr₂NH (0.27 mL, 1.93 mmol, 3.0 equiv) in THF (7 mL) was added a solution of n-BuLi (0.89 mL, 2.17 M/hexanes, 1.93 mmol, 3.0 equiv) dropwise at −78° C. The reaction was allowed to warm to 0° C., stirred at 0° C. for 25 min, and then cooled to −78° C. TMEDA (0.29 mL, 1.93 mmol, 3.0 equiv) was added, and the mixture was stirred at −78° C. for 5 min. A solution of compound S10-6 (0.51 g, 0.83 mmol, 1.3 equiv) in THF (0.5 mL) was added dropwise to the LDA solution over 5 min. Once addition was complete, the reaction mixture was stirred at −78° C. for 30 min. A solution of enone S1-9 (0.31 g, 0.64 mmol, 1.0 equiv) in THF (0.5 mL) was added dropwise over 2 min. The mixture was slowly warmed to −20° C. over 40 min and quenched by phosphate buffer (pH 7, 10 mL). The aqueous layer was extracted with EtOAc (20 mL×3). All organic layers were combined, dried (sodium sulfate), and concentrated. The residue was purified by flash chromatography on silica gel, eluting with hexanes/EtOAc (20:1 to 3:1) to afford 0.56 g of compound S10-7 (87%): ¹H NMR (400 MHz, CDCl₃) δ 15.96 (s, 1H), 7.50-7.24 (comp, 10H), 5.35 (s, 2H), 4.93 (d, J=9.8 Hz, 1H), 4.83 (d, J=9.8 Hz, 1H), 3.94 (d, J=12.2 Hz, 1H), 3.35 (s, 2H), 3.31-3.23 (m, 1H), 3.05-2.94 (m, 1H), 2.49 (s, 6H), 2.59-2.36 (comp, 3H), 2.21 (s, 6H), 2.20-2.10 (m, 1H), 1.37 (s, 9H), 1.32 (s, 9H), 0.81 (s, 9H), 0.27 (s, 3H), 0.12 (s, 3H); MS (ESI) m/z 997.42 (M+H).

To a solution of compound S10-7 in 1,4-dioxane (4 mL) was added HCl in 1,4-dioxane (4 N, 4 mL). The mixture was stirred for 3 hrs and then concentrated under reduced pressure. Potassium phosphate dibasic solution (prepared from 2 g K₂HPO₄ and 5 mL water) was added. The mixture was extracted with EtOAc (10 mL×3). All organic layers were combined, dried (sodium sulfate) and concentrated to afford 0.45 g (quant. yield) of compound S10-8: ¹H NMR (400 MHz, CDCl₃) δ 16.15 (s, 1H), 7.57-7.24 (comp, 10H), 5.35 (s, 2H), 4.90 (d, J=11.9 Hz, 1H), 4.82 (d, J=11.9 Hz, 1H), 3.97 (d, J=10.4 Hz, 1H), 3.50 (s, 2H), 3.17-3.09 (m, 1H), 3.03-2.93 (m, 1H), 2.48 (s, 6H), 2.18 (s, 6H), 2.57-2.08 (comp, 4H), 0.82 (s, 9H), 0.27 (s, 3H), 0.13 (s, 3H); MS ESI) m/z 797.36 (M+H).

To a solution of compound S10-8 (14 mg, 0.020 mmol, 1.0 equiv) and Et₃N (37 μL, 0.26 mmol, 13 equiv) in dichloromethane (2 mL) was added a solution of AcCl (2.2 μL, 0.030 mmol, 1.5 equiv) in dichloromethane (0.4 mL). The reaction was stirred at rt for 1 h, quenched by MeOH (0.3 mL), and then concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 2.0 mL (CH₃CN); gradient: 30→70% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 6.2-7.0 min, were collected and freeze-dried to give 14 mg of 510-9-1 (96%): ¹H NMR (400 MHz, CDCl₃) δ 15.98 (s, 1H), 7.54-7.24 (comp, 10H), 5.35 (s, 2H), 4.96-4.83 (m, 2H), 3.94 (d, J=9.8 Hz, 1H), 3.47 (s, 2H), 3.26-3.18 (m, 1H), 3.05-2.95 (m, 1H), 2.49 (s, 6H), 2.23 (s, 6H), 2.60-2.10 (comp, 4H), 2.00 (s, 3H), 0.82 (s, 9H), 0.27 (s, 3H), 0.13 (s, 3H); MS (ESI) m/z 839.34 (M+H).

To a solution of S10-9-1 (14 mg, 0.020 mmol) in 1,4-dioxane (3 mL) was added a solution of HF (0.3 mL of 48-50% solution in water). The mixture was stirred for 4 hrs and then quenched by potassium phosphate dibasic solution (prepared from 2 g K₂HPO₄ and 5 mL water). The mixture was extracted with EtOAc (5 mL×3). All organic layers were combined, dried (sodium sulfate) and concentrated.

To the above residue in MeOH/dioxane solution (1:1, 4 mL) was added Pd—C (10 mg, 10 wt %) and HCl in MeOH (0.5 N, 0.3 mL). The reaction was bubbled with H₂ (balloon) at 25° C. for 30 min. The mixture was filtered through a small Celite plug. The filtrate was concentrated to yield the crude product. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-□ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN injection volume: 2.1 mL (0.05 N HCl/water); gradient: 0→100% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 5.1-5.3 min, were collected and freeze-dried to yield 1 mg of S10-10-1 (11% for 2 steps): ¹H NMR (400 MHz, CD₃OD) δ4.36 (s, 2H), 4.10 (s, 1H), 3.05 (s, 3H), 2.96 (s, 3H), 2.94 (s, 6H), 3.22-2.88 (comp, 3H), 2.45-2.34 (m, 1H), 2.28 (s, 3H), 2.28-2.21 (m, 1H), 1.73-1.61 (m, 1H); MS (ESI) m/z 547.22 (M+H).

S10-10-2 was prepared according to the procedure for the preparation of S10-10-1: ¹H NMR (400 MHz, CD₃OD) δ 4.30 (s, 2H), 4.09 (s, 1H), 3.05 (s, 3H), 2.96 (s, 3H), 2.94 (s, 6H), 3.22-2.90 (comp, 3H), 2.46-2.36 (m, 1H), 2.29-2.20 (comp, 1H), 1.74-1.62 (m, 1H), 1.39 (s, 9H); MS (ESI) m/z 589.18 (M+H).

S10-10-3 was prepared according to the procedure for the preparation of S10-10-1: ¹H NMR (400 MHz, CD₃OD) δ 4.49 (s, 2H), 4.47 (s, 2H), 4.11 (s, 1H), 3.86-3.76 (m, 2H), 3.38-3.32 (m, 2H), 3.04 (s, 3H), 2.99 (s, 3H), 2.96 (s, 6H), 3.24-2.90 (comp, 3H), 2.45-2.36 (m, 1H), 2.30-2.00 (comp, 5H), 1.72-1.60 (m, 1H); MS (ESI) m/z 616.25 (M+H).

To a solution of compound S10-8 (59 mg, 0.070 mmol, 1.0 equiv) and Et₃N (0.15 mL, 1.11 mmol, 16 equiv) in dichloromethane (2 mL) was added methanesulfonic anhydride (28 mg, 0.30 mmol, 4.3 equiv). The reaction was stirred at rt for 1 h. The mixture was purified by flash chromatography on silica gel, eluting with hexanes/EtOAc (20:1 to 2:1) to afford 10 mg of compound S10-9-2 (15%): ¹H NMR (400 MHz, CDCl₃) δ 16.01 (s, 1H), 7.54-7.24 (comp, 10H), 5.35 (s, 2H), 4.96 (s, 2H), 3.93 (d, J=10.4 Hz, 1H), 3.68 (d, J=13.7 Hz, 1H), 3.62 (d, J=13.7 Hz, 1H), 3.24-3.17 (m, 1H), 3.00 (s, 3H), 3.06-2.98 (m, 1H), 2.48 (s, 6H), 2.27 (s, 6H), 2.59-2.11 (comp, 4H), 0.83 (s, 9H), 0.27 (s, 3H), 0.14 (s, 3H); MS (ESI) m/z 875.38 (M+H).

S10-10-4 was prepared from S10-9-2 according to the procedure for the preparation of S10-10-1: ¹H NMR (400 MHz, CD₃OD) δ 4.67 (d, J=13.4 Hz, 1H), 4.63 (d, J=13.4 Hz, 1H), 4.10 (s, 1H), 3.11 (s, 3H), 3.04 (s, 3H), 2.99 (s, 3H), 2.96 (s, 6H), 3.24-2.90 (comp, 3H), 2.46-2.35 (m, 1H), 2.29-2.22 (comp, 1H), 1.72-1.61 (m, 1H); MS (ESI) m/z 583.10 (M+H).

To a solution of compound S10-8 (27 mg, 0.030 mmol, 1.0 equiv) and acetic acid (6 μL, 0.10 mmol, 3.3 equiv) in 1,2-dichloroethane (1 mL) was added isobutyraldehyde (16 μL, 0.17 mmol, 5.6 equiv). The mixture was stirred at rt for 2 hrs. Na(OAc)₃BH (22 mg, 0.10 mmol, 3.3 equiv) was added, and the reaction was stirred for 3 hrs. Another portion of Na(OAc)₃BH (22 mg, 0.10 mmol, 3.3 equiv) was added, and the reaction was stirred for another 3 hrs. The mixture was diluted with EtOAc (10 mL). The organic layer was washed four times with potassium phosphate dibasic solution (prepared from 0.5 g K₂HPO₄ and 1 mL water), dried (sodium sulfate) and concentrated. The residue was purified by flash chromatography on silica gel, eluting with hexanes/EtOAc (20:1 to 10:1) to afford 23 mg of compound S10-9-3 (80%): ¹H NMR (400 MHz, CDCl₃) δ 16.27 (s, 1H), 7.55-7.24 (comp, 10H), 5.83-5.56 (br, 1H), 5.35 (s, 2H), 4.89 (d, J=10.4 Hz, 1H), 4.81 (d, J=10.4 Hz, 1H), 3.97 (d, J=10.4 Hz, 1H), 3.48 (s, 2H), 3.22-3.07 (comp, 3H), 2.97-2.87 (m, 1H), 2.48 (s, 6H), 2.55-2.30 (comp, 3H), 2.23 (s, 6H), 2.14-2.06 (m, 1H), 1.78-1.66 (m, 1H), 0.89 (d, J=1.8 Hz, 3H), 0.87 (d, J=1.8 Hz, 3H), 0.82 (s, 9H), 0.26 (s, 3H), 0.13 (s, 3H); MS (ESI) m/z 853.39 (M+H).

S10-10-5 was prepared from S10-9-3 according to the procedure for the preparation of S10-10-1: ¹H NMR (400 MHz, CD₃OD) δ 4.51 (s, 2H), 4.08 (s, 1H), 3.03 (s, 3H), 2.96 (s, 3H), 2.90 (s, 6H), 3.21-2.82 (comp, 5H), 2.36-2.25 (m, 1H), 2.25-2.17 (m, 1H), 1.93-1.81 (m, 1H), 1.69-1.58 (m, 1H), 1.04 (d, J=2.4 Hz, 3H), 1.02 (d, J=2.4 Hz, 3H); MS (ESI) m/z 561.18 (M+H).

S10-10-6 was prepared from S10-8 via HF treatment followed by hydrogenation according to the procedure for the preparation of S10-10-1: ¹H NMR (400 MHz, CD₃OD) δ 4.42 (s, 2H), 4.08 (s, 1H), 3.14 (dd, J=21.4, 4.9 Hz, 1H), 3.03 (s, 3H), 2.96 (s, 3H), 2.92 (s, 6H), 3.09-2.88 (comp, 2H), 2.26-2.17 (comp, 2H), 1.66-1.54 (m, 1H); MS (ESI) m/z 505.12 (M+H).

Example 11. Synthesis of Compounds Via Scheme 11

The following compounds were prepared according to Scheme 11.

Compound S1-7 (0.44 g, 1.00 mmol, 1.0 equiv) was dissolved in 1,2-dichloroethane (5 mL) and pyrrolidine (0.25 mL, 3.00 mmol, 3.0 equiv) was added via a syringe followed by acetic acid (0.17 mL, 3.00 mmol, 3.0 equiv) under a nitrogen atmosphere. After stirring at rt for 1 h, sodium triacetoxyborohydride (0.32 g, 1.50 mmol, 1.5 equiv) was added to reaction mixture. The reaction was stirred at rt overnight. Another 1.0 equiv of reagents were added. LC/MS indicated that the starting material was consumed after stirring for another 2 hrs. The reaction mixture was diluted with dichloromethane, washed with NaHCO₃ (saturated aqueous solution, 3×20 mL) and brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure to give crude compound S11-1-1 (0.53 g), which was used without further purification: ¹H NMR (400 MHz, CDCl₃) δ 10.37 (s, 1H), 7.53-7.49 (m, 2H), 7.40-7.33 (m, 5H), 7.26 (t, J=7.8 Hz, 1H), 7.09-7.05 (m, 2H), 5.13 (s, 2H), 3.94 (d, J=2.7 Hz, 2H), 2.66 (br, s, 2H), 2.35 (d, J=2.3 Hz, 3H), 1.82-1.76 (m, 2H).

Isopropylmagnesium chloride/lithium chloride (0.30 mL, 1.2 M/THF, 0.36 mmol, 1.8 equiv) was added to compound S11-1-1 (0.10 g, 0.20 mmol, 1.0 equiv) in THF (2 mL) under a nitrogen atmosphere at −78° C. The resulting solution was warmed up to rt and stirred for another 45 min. After the solution was cooled down to −78° C., TsN₃ (91 mg, 0.50 mmol, 2.5 equiv) in THF (1 mL) was added slowly via a syringe. The reaction was warmed to −40° C. and stirred for another 45 min. LC/MS indicated that the starting materials was consumed and the product present. The reaction mixture was allowed to slowly warm to −20° C. Saturated aqueous sodium bicarbonate (10 mL) was added. The resulting mixture was extracted with dichloromethane (3×15 mL), and the combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure to yield crude S11-2-1 (0.15 g) as a pale yellow oil.

Compound S11-2-1 (crude, 0.15 g, 0.20 mmol, 1.5 equiv) and enone S1-9 (64 mg, 0.13 mmol, 1.0 equiv) were dissolved in 2 mL dry THF under a nitrogen atmosphere in a flame dried schenck flask at −78° C. LHMDS (0.40 mL, 1.0 M/THF, 0.40 mmol, 3.1 equiv) was added slowly via a syringe. A red-orange color appeared. The reaction was warmed to −30° C. and stirred for another 15 min. Phosphate buffer (pH 7, 10 mL) was added, followed by the addition of 20 mL saturated aqueous ammonium chloride. The resulting mixture was extracted with dichloromethane (3×15 mL), and the combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure. The red-orange oil was purified by preparative reverse phase HPLC purification on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 4.0 mL (CH₃CN); gradient: 0→50% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product were collected and concentrated at rt to remove most of the acetonitrile. The resulting mostly aqueous solution was extracted with EtOAc. The EtOAc extract was dried (sodium sulfate) and concentrated to give 20 mg of compound S11-3-1 (12% for 3 steps).

Aqueous HF (0.3 mL, 48-50%) was added to a CH₃CN solution (1.0 mL) of S11-3-1 (20 mg) in a plastic vial at 25° C. The reaction was stirred at 25° C. for 18 hrs. The reaction solution was poured into an aqueous solution (10 mL) of K₂HPO₄ (2 g). The mixture was extracted with EtOAc (3×15 mL). The combined EtOAc extracts were dried over sodium sulfate and concentrated to give the crude intermediate (18 mg).

Pd—C (5 mg, 10 wt %) was added to a MeOH solution (2 mL) of the above crude intermediate. HCl in MeOH (0.5 N, 0.5 mL) was added. The reaction was stirred under H₂ (balloon) at 25° C. for 45 min and filtered through a pad of Celite. The filtrate was concentrated to afford the crude product, which was purified by reverse phase HPLC on a Polymerx 10μ RP-γ100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: CH₃CN, sample in 2.0 mL (0.05 N HCl), gradient elution with 0→70% B over 15 min, mass-directed fraction collection]. Fractions containing the desired product were collected and freeze-dried, yielding 8 mg of the desired product S1-4-1 as a yellow solid (65% for two steps): ¹H NMR (400 MHz, CD₃OD) δ 4.64 (s, 2H), 4.12 (s, 1H), 3.68 (m, 2H), 3.32 (m, 2H), 3.05 (s, 3H), 2.96 (s, 3H), 3.27-2.97 (m, 3H), 2.45 (m, 1H), 2.30-2.13 (m, 3H), 2.13-2.00 (m, 2H), 1.69-1.59 (m, 1H); MS (ESI) m/z 541.27 (M+H).

Example 12. Synthesis of Compounds Via Scheme 12

The following compounds were prepared according to Scheme 12.

iPrMgCl.LiCl (4.00 mL, 1.2 M/THF, 4.80 mmol, 2.0 equiv) was added to a THF solution (12 mL) of S1-6 (1.00 g, 2.40 mmol,) at 0° C. The reaction was stirred at 0° C. for 30 min. (PhSO₂)₂NF (1.50 g, 4.80 mmol, 2.0 equiv) was added to the reaction mixture. The reaction was stirred at 0° C. for 30 min and allowed to warm to 25° C. over 1 h, quenched by saturated aqueous NH₄Cl, and extracted with EtOAc. The EtOAc extract was dried (sodium sulfate) and concentrated to give the crude product. Flash chromatography on silica gel (30:1 hexanes/EtOAc) yielded 0.50 g of compound S12-1 (59%).

n-BuLi (6.50 mL, 1.6 M/hexanes, 10.40 mmol, 1.5 equiv) was added to a THF solution (15 mL) of diisopropylamine (1.50 mL, 10.40 mmol, 1.5 equiv) at 0° C. The reaction was stirred at 0° C. for 30 min and cooled to −78° C. To the mixture was added a THF solution (15 mL) of S12-1 (2.29 g, 6.47 mmol, 1.0 equiv). The reaction was stirred at −78° C. for 20 min and DMF (1.50 mL, 19.40 mmol, 3.0 equiv) was added. The reaction was allowed to warm to 25° C. over 1 h and quenched by saturated aqueous NH₄Cl. The resulting mixture was extracted with EtOAc. The EtOAc extract was dried over sodium sulfate and concentrated. Flash chromatography on silica gel (10:1 hexanes/EtOAc) yielded 1.70 g of compound S12-2 (69%).

Pyrrolidine (65 μL, 0.79 mmol, 5.0 equiv), Na(OAc)₃BH (68 mg, 0.32 mmol, 2.0 equiv), and HOAc (47 μL) were added to a dichloromethane solution (3 mL) of S12-2 (60 mg, 0.16 mmol, 1.0 equiv). The reaction was stirred at 25° C. for 1 h and quenched by H₂O. The resulting mixture was extracted with dichloromethane. The dichloromethane extract was dried (sodium sulfate) and concentrated to give crude S12-3-1.

A THF solution (1 mL) of crude S12-3-1 (0.14 mmol, 1.7 equiv) was added to a THF solution (1 mL) of LDA (0.17 mL, 1.8 M/THF/heptane/ethylbenzene, 0.31 mmol, 3.7 equiv) and TMEDA (84 μL, 0.56 mmol, 6.7 equiv). The reaction was stirred at −78° C. for 10 min. A THF solution (1 mL) of enone S1-9 (40 mg, 0.084 mmol, 1.0 equiv) was added to the reaction at −78° C. The reaction was stirred at −78° C. for 30 min and allowed to warm to 25° C. over 1 h, quenched by saturated aqueous NH₄Cl, and extracted with EtOAc. The EtOAc extract was dried (sodium sulfate) and concentrated. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 4.0 mL (CH₃CN); gradient: 50→100% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product were collected and concentrated at 25° C. to remove most of the acetonitrile. The resulting aqueous solution was extracted with EtOAc, and the EtOAc extract was dried (sodium sulfate) and concentrated to give 16 mg of S12-4-1 (23%).

Aqueous HF (0.5 mL, 48-50%) was added to a CH₃CN solution (2 mL) of S12-4-1 (16 mg, 0.019 mmol) in a polypropylene tube at 25° C. The reaction was stirred at 25° C. for 18 hrs. The resulting mixture was poured into an aqueous solution of K₂HPO₄ (4 g, dissolved in 30 mL water). The mixture was extracted with EtOAc, and the EtOAc extract was dried (sodium sulfate) and concentrated to yield the crude intermediate.

Pd—C (8 mg, 10 wt %) was added to a HCl/MeOH solution (0.5N, 2 mL) of the above crude product. The reaction was purged with hydrogen and stirred under H₂ (balloon) at 25° C. for 4 hrs. The reaction mixture was filtered through a small Celite plug. The filtrate was concentrated to yield the crude product, which was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 ml/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 0→70% B over 7 min, 70→100% over 3 min, and 100% over 5 min; mass-directed fraction collection]. Fractions containing the desired product were collected and freeze-dried to yield 2 mg of compound S12-5-1: ¹H NMR (400 MHz, CD₃OD) δ 4.58 (s, 2H), 4.10 (s, 1H), 3.71-3.67 (m, 2H), 3.30-2.92 (m, 5H), 3.05 (s, 3H), 2.97 (s, 3H), 2.41-2.32 (m, 1H), 2.29-2.15 (m, 3H), 2.10-1.98 (m, 2H), 1.72-1.60 (m, 1H); MS (ESI) m/z 534.23 (M+H).

The following compounds were prepared similarly to S12-5-1.

S12-5-2:

¹H NMR (400 MHz, CD₃OD) δ 4.43 (s, 2H), 4.08 (s, 1H), 3.29-2.92 (m, 3H), 3.04 (s, 3H), 3.00 (s, 2H), 2.96 (s, 3H), 2.39-2.28 (m, 1H), 2.26-2.19 (m, 1H), 1.70-1.59 (m, 1H), 1.06 (s, 9H); MS (ESI) m/z 550.16 (M+H).

S12-5-3:

¹H NMR (400 MHz, CD₃OD) δ 4.32 (s, 2H), 4.10 (s, 1H), 3.24-2.93 (m, 3H), 3.04 (s, 3H), 2.95 (s, 3H), 2.39-2.20 (m, 2H), 1.70-1.58 (m, 1H), 1.48 (s, 9H), MS (ESI) m/z 536.24 (M+H).

S12-5-4:

¹H NMR (400 MHz, CD₃OD) δ 4.19 (s, 2H), 4.11 (s, 1H), 3.25-2.95 (m, 3H), 3.06 (s, 3H), 2.96 (s, 3H), 2.80 (s, 3H), 2.42-2.21 (m, 2H), 1.71-1.58 (m, 1H), 1.58 (s, 9H); MS (ESI) m/z 550.26 (M+H).

Example 13. Synthesis of Compounds Via Scheme 13

The following compounds were prepared according to Scheme 13.

A solution of benzoic acid S13-1 (5.00 g, 26.60 mmol, 1.0 equiv) in THF (40 mL) was added dropwise to a solution of s-BuLi (41.76 mL, 1.4 M/cyclohexane, 58.50 mmol, 2.2 equiv) and TMEDA (8.77 mL, 58.50 mmol, 2.2 equiv) in THF (40 mL) at −78° C. via a cannula over 30 min. The resulting red-orange thick reaction mixture was stirred at −78° C. for 2 hrs, slowly changing to a yellow brownish suspension. Iodomethane (8.30 mL, 133.00 mmol, 5.0 equiv) was added at −78° C. slowly. The resulting yellow suspension was then stirred at rt for 30 min. Water (50 mL) was added, and the resulting mixture was concentrated to remove most of the THF. Aqueous NaOH (40 mL, 6 N) was added, and the resulting mixture was extracted with methyl I-butyl ether (2×60 mL). The combined organic phases were extracted with aqueous NaOH (40 mL, 3 N). The combined aqueous layers were acidified with 6 N HCl (−65 mL) to pH 1, and extracted with EtOAc (120 mL, then 80 mL). The combined EtOAc extracts were dried over magnesium sulfate, filtered and concentrated to afford crude S13-2 as an orange solid (S13-2:region-isomer:S13-1=˜1.2:0.8:1 by ¹H NMR).

The above crude product was dissolved in dry dichloromethane (100 mL). Oxalyl chloride (2.78 mL, 31.89 mmol, 1.2 equiv) was added, followed by a few drops of DMF. The reaction mixture was stirred at rt for 1 h, concentrated and further dried under high vacuum. The residue was re-dissolved in dry dichloromethane (100 mL). Phenol (2.75 g, 29.26 mmol, 1.1 equiv), triethylamine (7.40 mL, 53.20 mmol, 2.0 equiv), and DMAP (0.10 g, 0.82 mmol, 0.03 equiv) were added. The reaction mixture was stirred at rt for 1 h. The solvent was evaporated and the residue was dissolved in EtOAc (200 mL). The organic layer was washed with 1 N HCl (60 mL), 1 N NaOH (70 mL), water (50 mL) and brine (60 mL), dried over anhydrous magnesium sulfate, filtered, and concentrated. The residue was purified by flash column chromatography (1-4% EtOAc/hexanes) to afford the desired product S13-3 as a white solid (1.39 g, 19% over two steps): ¹H NMR (400 MHz, CDCl₃) δ 7.47-7.43 (m, 2H), 7.31-7.29 (m, 1H), 7.26-7.24 (m, 2H), 6.67 (dd, J=6.0, 11.4 Hz, 1H), 3.84 (s, 3H), 2.41 (s, 3H); MS (ESI) m/z 277.22 (M−H).

A solution of BBr₃ in dichloromethane (6.00 mL, 1.0 M, 6.00 mmol, 1.2 equiv) was added slowly to compound S13-3 (1.39 g, 5.00 mmol, 1.0 equiv) in dichloromethane (25 mL) at −78° C. The resulting orange solution was allowed to warm to 0° C. in 30 min and kept at that temperature for 10 min [monitored by LC-MS or TLC (product is slightly more polar)]. Saturated aqueous NaHCO₃ was added. The mixture was stirred at rt for 5 min and dichloromethane was evaporated. The residue was extracted with EtOAc (60 mL, then 20 mL). The organic extracts were combined, dried over anhydrous magnesium sulfate, filtered, and concentrated to give the crude intermediate (1.27 g) as a waxy solid: ¹H NMR (400 MHz, CDCl₃) δ 11.07 (s, 1H), 7.47-7.44 (m, 2H), 7.34-7.30 (m, 1H), 7.19-7.17 (m, 2H), 6.69 (dd, J=6.7, 11.0 Hz, 1H), 2.63 (d, J=3.0 Hz, 3H); MS (ESI) m/z 263.20 (M−H).

Benzylbromide (0.24 mL, 2.04 mmol, 1.2 equiv), K₂CO₃ powder (0.47 g, 3.41 mmol, 2.0 equiv) and KI (14 mg, 0.085 mmol, 0.05 equiv) were added to the above crude intermediate (0.45 g, 1.70 mmol, 1.0 equiv) in acetone (3.4 mL). The mixture was heated at reflux for 4.5 hrs, cooled to it, and diluted with EtOAc (50 mL) and water (30 mL). The organic phase was separated, washed with brine, dried over magnesium sulfate, and concentrated. The residue was purified by flash column chromatography (1-4% EtOAc/hexanes) to afford the desired product S13-4 as a crystalline white solid (0.50 g, 79% over 2 steps): ¹H NMR (400 MHz, CDCl₃) δ 7.45-7.31 (m, 7H), 7.28-7.24 (m, 1H), 7.10-7.08 (m, 2H), 6.71 (dd, J=6.1, 11.6 Hz, 1H), 5.10 (s, 2H), 2.41 (d, J=1.8 Hz, 3H); MS (ESI) m/z 353.21 (M−H).

A solution of compound S13-4 (0.38 g, 1.08 mmol, 1.0 equiv) in THF (4 mL) was added via a cannula dropwise to a solution of LDA (1.01 mL, 1.62 mmol, 1.5 equiv) and TMEDA (0.24 mL, 1.62 mmol, 1.5 equiv) in THF (6 mL) at −78° C. The resulting red orange solution was stirred at −78° C. for 5 min. DMF (0.17 mL, 2.16 mmol, 2.0 equiv) was added. The reaction was stirred at −78° C. for 1 h. Saturated aqueous NH₄Cl (30 mL) was added dropwise to the reaction mixture at −78° C. The resulting mixture was then warmed up to rt and extracted with EtOAc (60 mL, then 20 mL). The combined EtOAc extracts were dried (sodium sulfate), filtered and concentrated. The residue was purified by flash column chromatography (1-20% EtOAc/hexanes) to afford the desired product S13-5 as a colorless oil (0.26 g, 62%): ¹H NMR (400 MHz, CDCl₃) δ 7.42-7.35 (m, 7H), 7.29-7.25 (m, 1H), 7.06-7.04 (m, 2H), 5.10 (s, 2H), 2.48 (d, J=2.4 Hz, 3H); MS (ESI) m/z 381.24 (M−H).

t-BuNH₂ (96 μL, 0.91 mmol, 5.0 equiv) was added to a mixture of aldehyde S13-5 (69 mg, 0.18 mmol, 1.0 equiv) and sodium sulfate in toluene (1 mL). The resulting light greenish reaction mixture was stirred at rt overnight. The solids were filtered off. The filtrate was concentrated under reduced pressure to afford the crude imine, which was used directly in the next step.

A solution of the above imine (0.18 mmol, 1.0 equiv) in THF (1.5 mL) was added via a cannula dropwise to a solution of LDA (0.16 mL, 0.26 mmol, 1.4 equiv) and TMEDA (38 μL, 0.26 mmol, 1.4 equiv) in THF (4 mL) at −78° C. The resulting red-purple reaction mixture was then stirred at −78° C. for 5 min. A solution of enone S1-9 (70 mg, 0.15 mmol, 0.8 equiv) in THF (1.5 mL) was added via a cannula. The resulting dark purple solution was stirred at −78° C. for 8 min, and LHMDS solution (0.18 mL, 1.0 M/THF, 0.18 mmol, 1.0 equiv) was added. The reaction mixture was warmed to −10° C. over 1 h 25 min, quenched by saturated aqueous NH4Cl (20 mL), and extracted with EtOAc (50 mL, then 20 mL). The combined EtOAc extracts were dried (sodium sulfate), filtered and concentrated. The residue was purified by a preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 ml/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 4.0 mL (CH₃CN); gradient: 80→100% B in A over 10 min; mass-directed fraction collection]. Fractions containing the desired product were collected and concentrated at rt to yield the desired product S13-7 (23 mg, 20%): ¹H NMR (400 MHz, CDCl₃) δ 15.85 (br s, 1H), 10.10 (s, 1H), 7.70-7.68 (m, 1H), 7.52-7.49 (m, 3H), 7.40-7.31 (m, 6H), 5.36 (s, 2H), 5.06, 4.98 (ABq, J=10.4 Hz, 2H), 3.91 (d, J=11.0 Hz, 1H), 3.34 (dd, J=4.3, 15.9 Hz, 1H), 3.07-3.00 (m, 1H), 2.61-2.58 (m, 1H), 2.54-2.34 (m, 2H), 2.50 (s, 6H), 2.20-2.16 (m, 1H), 0.82 (s, 9H), 0.28 (s, 3H), 0.14 (s, 3H); MS (ESI) m/z 771.53 (M+H).

Neopentylamine (17 μL, 0.15 mmol, 5.0 equiv), acetic acid (8 μL, 0.15 mmol, 5.0 equiv) and sodium triacetoxyborohydride (12 mg, 0.058 mmol, 2.0 equiv) were added sequentially to a solution of aldehyde S13-7 (23 mg, 0.029 mmol, 1.0 equiv) in 1,2-dichloroethane (1 mL) at 23° C. After stirring for 1 h, the reaction mixture was quenched by the addition of saturated aqueous sodium bicarbonate and pH 7 phosphate buffer (1:1, 15 mL) and extracted with dichloromethane (50 mL). The organic phase was dried over anhydrous sodium sulfate, filtered, and concentrated to afford crude S13-8-1, which was used directly in the next step.

Aqueous HF (48-50%, 0.3 mL) was added to a solution of compound S13-8-1 in acetonitrile (0.7 mL) in a polypropylene reaction vessel at 23° C. The reaction was stirred vigorously at 23° C. overnight and poured into aqueous K₂HPO₄ (3.6 g dissolved in 20 mL water). The mixture was extracted with EtOAc (2×25 mL). The combined organic extracts were dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was used directly in the next step without further purification.

Pd—C (10 wt %, 7 mg) was added into a solution of the above crude product in a mixture of HCl/MeOH (0.5 N, 0.12 mL, 2.0 equiv) and MeOH (2 mL) at 23° C. The reaction vessel was sealed and purged with hydrogen by briefly evacuating the flask followed by flushing with hydrogen gas (1 atm). The reaction was stirred at 23° C. for 30 min. The reaction mixture was then filtered through a small Celite pad. The filtrate was concentrated. The residue was purified by a preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-□ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 5→40% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 12.6-13.1 min, were collected and freeze-dried to yield compound S13-9-1 (4 mg, 23% for 3 steps): ¹H NMR (400 MHz, CD₃OD) δ 4.38 (s, 2H), 4.10 (s, 1H), 3.11-2.96 (m, 11H), 2.39 (t, J=15.1 Hz, 1H), 2.26-2.23 (m, 1H), 1.70-1.60 (m, 1H), 1.07 (s, 9H); MS (ESI) m/z 550.40 (M+H).

S13-9-2 was prepared similarly: ¹H NMR (400 MHz, CD₃OD) δ 4.31 (s, 2H), 4.11 (s, 1H), 3.57-2.50 (hept, J=6.4 Hz, 1H), 3.04-2.96 (m, 9H), 2.39 (t, J=15.1 Hz, 1H), 2.27-2.24 (m, 1H), 1.70-1.60 (m, 1H), 1.43 (t, J=6.4 Hz, 6H); MS (ESI) m/z 522.41 (M+H).

Example 14. Synthesis of Compounds Via Scheme 14

The following compounds were prepared according to Scheme 14.

To compound S1-1 (5.00 g, 29.38 mmol, 1.0 equiv) in sulfuric acid (10 mL) and trifluoroacetic acid (20 mL) at rt was added NBS (5.75 g, 32.30 mmol, 1.1 equiv). The pale solution was stirred at rt for 3 hrs. The resulting pale suspension was carefully poured onto 500 g crushed ice. The mixture was extracted with EtOAc (200 mL×1, 100 mL×3). The EtOAc extracts were combined, dried over sodium sulfate, and concentrated under reduced pressure. The light yellow residue was suspended in minimum dichloromethane. The solid was collected, washed with cold dichloromethane (5 mL×3), and dried under vacuum overnight to yield the desired product S14-1 as a white crystalline solid (5.49 g, 75%): R_(f) 0.55 (0.5% HOAc/EtOAc); ¹H NMR (400 MHz, CDCl₃) δ 7.74-7.77 (m, 1H), 7.24-7.55 (m, 1H), 4.01 (s, 3H); MS (ESI) m/z 247.1 (M−H), calcd for CsH₆BrFO₃ 248.0.

To compound S14-1 (0.25 g, 1.00 mmol, 1.0 equiv) in anhydrous THF (5 mL) at −78° C. was added TMEDA (0.75 mL, 5.00 mmol, 5.0 equiv) and LTMP (4.00 mmol, freshly prepared, in 4 mL THF) dropwise. The cloudy deep red solution was stirred at −78° C. for 30 min. Iodomethane (0.31 mL, 5.00 mmol, 5.0 equiv) was added dropwise at −78° C. The resulting pale suspension was allowed to warm to rt and was stirred overnight. Aqueous HCl (1 N) was added to the reaction until pH 1-2. The mixture was extracted with EtOAc (100 mL×1, 20 mL×3). The EtOAc extracts were combined, dried over sodium sulfate, and concentrated under reduced pressure to afford crude S14-2 as an orange solid (0.30 g)

The compound S14-2 was dissolved in dry dichloromethane (10 mL). Anhydrous DMF (2 drops) and oxalyl chloride (0.43 mL, 5.00 mmol, 5.0 equiv) were added dropwise at rt. The yellow solution was stirred at rt for 30 min and concentrated under reduced pressure. The residue was redissolved in dry dichloromethane (10 mL). Phenol (0.14 g, 1.50 mmol, 1.5 equiv), DIEA (0.70 mL, 4.00 mmol, 4.0 equiv), and DMAP (12 mg, 0.10 mmol, 0.1 equiv) were added. The reaction was stirred at rt overnight, diluted with saturated aqueous sodium bicarbonate (50 mL), and extracted with dichloromethane (50 mL×3). The dichloromethane extracts were combined, dried over sodium sulfate, and concentrated under reduced pressure. Flash column chromatography on silica gel using 0% to 5% EtOAc/hexanes yielded the desired product S14-3 as a pale oil (0.13 g, 37%, 2 steps): R_(f) 0.70 (10% EtOAc/hexanes); ¹H NMR (400 MHz, CDCl₃) δ 7.40-7.50 (m, 2H), 7.16-7.23 (m, 3H), 3.94 (s, 3H), 2.38 (s, 3H), 2.32 (s, 3H); MS (ESI) m/z 351.1 (M−H), calcd for C₁₆H₁₄BrFO₃ 352.0.

Compound S14-3 (0.13 g, 0.37 mmol, 1.0 equiv) was dissolved in anhydrous dichloromethane (4 mL) and the solution was cooled to −78° C. BBr₃ (0.93 mL, 1.0 M/dichloromethane, 0.93 mmol, 2.5 equiv) was added dropwise. The orange-brown solution was warmed to −10° C. with stirring over a period of 2 hrs. The reaction was quenched by saturated aqueous sodium bicarbonate (20 mL) and extracted with EtOAc (20 mL×3). The extracts were combined, dried over sodium sulfate, and concentrated under reduced pressure to yield crude S14-4 as a pale solid (0.13 g).

Phenol S14-4 was dissolved in dichloromethane. Di-t-butyl dicarbonate (0.12 g, 0.56 mmol, 1.5 equiv), DIEA (0.13 mL, 0.74 mmol, 2.0 equiv), and DMAP (5 mg, 0.040 mmol, 0.1 equiv) were added. The reaction was stirred at rt overnight and concentrated under reduced pressure. Flash column chromatography on silica gel using 0% to 2% EtOAc/hexanes yielded the desired compound S14-5 as a white solid (0.14 g, 88%, 2 steps): R_(f) 0.25 (3% EtOAc/hexanes); ¹H NMR (400 MHz, CDCl₃) δ 77.40-7.49 (m, 2H), 7.18-7.32 (m, 3H), 2.42 (d, J=3.0 Hz, 3H), 2.38 (d, J=2.5 Hz, 3H), 1.45 (s, 9H); MS (ESI) m/z 437.1 (M−H), calcd for C₂₀H₂₀BrFO₃ 438.0.

To diisopropylamine (0.090 mL, 0.64 mmol, 5.0 equiv) in anhydrous THF at −78° C. was added n-BuLi (0.40 mL, 1.6 M/hexanes, 0.64 mmol, 5.0 equiv). The colorless solution was stirred at 0° C. for 10 min and cooled to −78° C. TMEDA (0.29 mL, 1.92 mmol, 15 equiv) was added, followed by the dropwise addition of compound S14-5 (0.14 g, 0.32 mmol, 2.5 equiv, in 3 mL THF) over a period of 5 min. The deep red solution was stirred at −78° C. for 15 min. Enone S1-9 (62 mg, 0.13 mmol, 1.0 equiv, in 3 mL THF) was added. The reaction was warmed from −78° C. to −10° C. with stirring over a period of 30 min, quenched by HOAc (0.12 mL) and saturated aqueous ammonium chloride (50 mL), and extracted with EtOAc (100 mL×1, 50 mL×2). The EtOAc extracts were combined, dried over sodium sulfate, and concentrated under reduced pressure. Reverse phase HPLC purification yielded the desired product S14-6 as a light-yellow solid (36 mg, 34%): R_(f) 0.45 (20%. EtOAc/hexanes); ¹H NMR (400 MHz, CDCl₃) δ 15.69 (br s, 1H), 7.45-7.50 (m, 2H), 7.30-7.40 (m, 3H), 5.34 (s, 2H), 3.97 (br s, 1H), 3.20-3.30 (br m, 1H), 2.98-3.07 (br m, 1H), 2.1-2.7 (m, 11H), 1.5-1.6 (m, 11H), 0.80 (s, 9H), 0.23 (s, 3H), 0.11 (s, 3H); MS (ESI) m/z 827.3 (M+H), calcd for C₄₀H₄₈BrFN₃O₉Si 826.2.

Compound S14-6 (36 mg, 0.044 mmol) was dissolved in acetonitrile (1.5 mL). Aqueous HF (0.75 mL, 48-50%) was added. The yellow solution was stirred at rt overnight, diluted with aqueous K₂HPO₄ (3.2 g in 16 mL water), and extracted with dichloromethane (20 mL×3). The dichloromethane extracts were combined, dried over sodium sulfate, and concentrated under reduced pressure to yield crude S14-7 as a bright-yellow solid (31 mg): MS (ESI) m/z 613.2 (M+H), calcd for C₂₉H₂₆BrFN₂O₇ 612.1.

The above crude product S14-7 was dissolved in MeOH (4 mL) and dioxane (2 mL). 10% Pd—C (19 mg, 0.0090 mmol, 0.2 equiv) was added. The mixture was purged with hydrogen and stirred under 1 atm hydrogen atmosphere at rt for 1 h. The catalyst was filtered off with a small Celite pad and washed with MeOH (5 mL×3). The yellow filtrate was concentrated under reduced pressure to yield crude S14-8 as a yellow solid (27 mg). 100% of the crude product was purified with reverse phase HPLC using similar conditions for S2-4-1 to yield compound S14-8 as a yellow solid (2 mg, 71%, 2 steps, HCl salt): ¹H NMR (400 MHz, CD₃OD) δ 6.69 (d, J=6.1 Hz, 1H), 4.06 (s, 1H), 2.80-3.50 (m, 3H), 3.03 (s, 3H), 2.95 (s, 3H), 2.10-2.40 (m, 2H), 2.27 (s, 3H), 1.56-1.68 (m, 1H); MS (ESI) m/z 447.1 (M+H), calcd for C₂₂H₂₃FN₂O₇ 446.2.

Compound S14-8 (90% of crude from above, 0.40 mmol) was dissolved in cold sulfuric acid (2 mL, 0° C.). Nitric acid (0.12 mL, 0.5 M in sulfuric acid, 0.060 mmol, 1.5 equiv) was added. The deep brown solution was stirred at 0° C. for 1 h. The resulting deep red solution was added dropwise into ether (100 mL) with stirring. The yellow solid was collected onto a small Celite pad, washed with ether (5 mL×4, discarded), and eluted with MeOH (5 mL×3). The yellow MeOH solution was collected and concentrated under reduced pressure to yield the desired compound S14-9 as a yellow solid (27 mg): MS (ESI) m/z 492.3 (M+H), calcd for C₂₂H₂₂FN₃O₉ 491.1.

Compound S14-9 (25 mg, 0.036 mmol) was dissolved in MeOH (4 mL). 10% Pd—C (15 mg, 0.0070 mmol, 0.2 equiv) was added. The mixture was purged with hydrogen and stirred at rt under 1 atm hydrogen atmosphere for 4 hrs. The catalyst was filtered off with a small Celite pad and washed with MeOH (5 mL×3). The yellow filtrate was concentrated under reduced pressure. HPLC purification using similar conditions for S2-4-1 yielded the desired product S14-10 as a deep red solid (6 mg, 31%, 3 steps, bis-HCl salt): ¹H NMR (400 MHz, CD₃OD) δ 4.09 (s, 1H), 2.80-3.50 (m, 3H), 3.03 (s, 3H), 2.95 (s, 3H), 2.30-2.40 (m, 1H), 2.34 (s, 3H), 2.19-2.27 (m, 1H), 1.60-1.62 (m, 1H); MS (ESI) m/z 462.1 (M+H), calcd C₂₂H₂₄FN₃O₇ 461.2.

Compound S14-10 (4 mg, 0.0075 mmol, 1.0 equiv) was dissolved in acetonitrile/DMPU (0.1 mL, 1:1 v/v). t-Butylaminoacetyl chloride (2 mg, HCl salt, 0.011 mmol, 1.5 equiv, prepared by treating t-butylaminoacetic acid with excess thionyl chloride followed by concentration under reduced pressure) was added. The deep red solution was stirred at rt for 3 hrs and quenched by aqueous HCl (2 mL, 0.1 N). HPLC purification using similar conditions for S2-4-1 yielded the desired product S14-11 as a yellow solid (2 mg, 41%, bis-HCl salt): ¹H NMR (400 MHz, CD₃OD) δ 4.08 (br s, 3H), 2.78-3.50 (m, 3H), 3.04 (s, 3H), 2.95 (s, 3H), 2.18-2.37 (m, 2H), 2.21 (s, 3H), 1.60-1.62 (m, 1H), 1.42 (s, 9H); MS (ESI) m/z 575.4 (M+H), calcd for C₂₈H₃₅FN₄O₈ 574.2.

Example 15. Synthesis of Compounds Via Scheme 15

The following compounds were prepared according to Scheme 15.

To an ice-cooled solution of 2-methoxy-6-methylaniline (S15-1, 25.12 g, 183.10 mmol, 1 equiv) in MeOH (79 mL) and HOAc (25 mL) was added a solution of bromine (9.41 mL, 183.10 mmol) in HOAc (79 mL) dropwise via an addition funnel. The reaction mixture was allowed to warm to rt and stirred for 2 hrs after complete addition. EtOAc (150 mL) was added, and the solid was collected by filtration and washed with more EtOAc, yielding 37.20 g of compound S15-2 as an off-white solid (HBr salt).

4-Bromo-2-methoxy-6-methylaniline (S15-2, HBr salt, 20.00 g, 92.70 mmol, 1.0 equiv) was suspended in concentrated HCl (22 mL) and crushed ice (76 g) cooled in an ice-bath. A solution of NaNO₂ (6.52 g, 94.60 mmol, 1.02 equiv) in H₂O (22 mL) was added dropwise. The resulting mixture was stirred at 0° C. for 30 min and neutralized with aqueous Na₂CO₃. A suspension of CuCN (10.4 g, 115.90 mmol, 1.25 equiv) in H₂O (44 mL) was mixed with a solution of NaCN (14.4 g, 294.80 mmol, 3.18 equiv) in 22 mL of H₂O, and cooled in an ice-bath. The initial diazonium salt mixture was then added to the CuCN and NaCN mixture with vigorous stirring while maintaining the temperature at 0° C. (toluene (180 mL) was also added in portions during the addition). The reaction mixture was stirred at 0° C. for 1 h, rt for 2 hrs, and 50° C. for another 1 h. After cooling to rt, the layers were separated. The aqueous layer was extracted with toluene. The combined organic layers were washed with brine, dried over magnesium sulfate, and concentrated. The residue was passed through a silica gel plug, washed with toluene, and concentrated to yield 14.50 g of compound S15-3 as a light yellow solid.

To a solution of S15-3 (11.34 g, 50.20 mmol, 1.0 equiv) in THF (100 mL) was added 1.5 M DIBAL-H in toluene (40.10 mL, 60.20 mmol, 1.2 equiv) slowly at −78° C. The reaction was allowed to warm to rt gradually and stirred overnight. After re-cooled to 0° C., the reaction was carefully quenched by 1 N HCl. The resulting mixture was stirred at rt for 1 h and extracted three times with EtOAc. The combined EtOAc layers were washed with H₂O, saturated aqueous NaHCO₃ and brine, dried over magnesium sulfate, and concentrated to provide compound S15-4 as a yellow solid, which was used directly in the next step.

To a suspension of S15-4 (50.20 mmol, 1.0 equiv) in t-BuOH (200 mL) was added a solution of NaClO₂ (11.34 g, 100.30 mmol, 2.0 equiv) and NaH₂PO₄ (34.6 g, 250.80 mmol, 5.0 equiv) in H₂O (100 mL) via an addition funnel. After complete addition, 2-methyl-2-butene (42.40 mL, 0.40 mol, 8 equiv)) was added. The resulting homogenous solution was stirred at rt for 30 min, and volatiles were removed. The residue was suspended in 150 mL of H₂O. The solution was acidified to pH ˜1 with 1 N HCl and extracted three times with t-butyl methyl ether. The combined organic solution was extracted three times with 1 N NaOH. The combined aqueous solution was acidified with 6 N HCl, and extracted three times with EtOAc. The combined EtOAc extracts were washed with brine, dried over magnesium sulfate, and concentrated to provide 8.64 g of the benzoic acid intermediate (15-4-a) as an off-white solid, which was used directly in the next step.

To a solution of the above benzoic acid (8.64 g, 35.20 mmol, 1.0 equiv) in dichloromethane (70 mL) was added oxalyl chloride (3.76 mL, 42.30 mmol, 1.2 equiv), followed by a couple of drops of DMF (caution: gas evolution). The mixture was stirred at it for 30 min and the volatiles were evaporated under reduce pressure. The residue was further dried under high vacuum to afford the crude benzoyl chloride. The crude benzoyl chloride was re-dissolved in dichloromethane (70 mL). Triethylamine (12.3 mL, 88.10 mmol, 2.5 equiv), phenol (3.98 g, 42.30 mmol, 1.2 equiv) and DMAP (0.43 g, 3.52 mmol, 0.1 equiv) were added. The mixture was stirred at rt for 1 h. The solvent was evaporated. The residue was suspended in EtOAc, and the precipitate was filtered off. The organic solution was then washed with 1 N HCl (three times), H₂O, saturated aqueous NaHCO₃, and brine, dried over sodium sulfate, filtered and concentrated. Purification of the residue by flash chromatography gave compound S15-5 (10.05 g, 89%) as an off-white solid: ¹H NMR (400 MHz, CDCl₃) δ 7.41-7.45 (m, 2H), 7.22-7.27 (m, 3H), 7.04 (d, J=0.9 Hz, 1H), 6.97 (d, J=0.9 Hz, 1H), 3.87 (s, 3H), 2.42 (s, 3H); MS (ESI) m/z 319.0 (M−H), calcd for C₁₅H₁₂BrO₃ 319.0.

To a solution of compound S15-5 (2.52 g, 7.87 mmol, 1.0 equiv) in acetonitrile (16 mL) was added NCS (1.10 g, 8.27 mmol, 1.05 equiv) in one portion. The resulting mixture was heated in a 60° C. oil bath for 45 hrs. The solvent was evaporated. The residue was redissolved in Et₂O (400 mL), washed with 1 N NaOH, H₂O and brine, dried over sodium sulfate, and concentrated to provide 2.76 g of compound S15-6 as a white solid. This material was used directly in the next step without further purification: ¹H NMR (400 MHz, CDCl₃) δ 7.44 (dd, J=7.8, 7.8 Hz, 2H), 7.22-7.28 (m, 3H), 7.13 (s, 1H), 3.87 (s, 3H), 2.51 (s, 3H); MS (ESI) m/z 353.0 (M−H), calcd for C₁₅H₁₁BrClO₃ 352.97.

Compound S15-6 (2.76 g, 7.76 mmol, 1.0 equiv) was dissolved in anhydrous dichloromethane (78 mL) and cooled to −78° C. A solution of boron tribromide (1.0 M in dichloromethane, 7.76 mL, 7.76 mmol, 1.0 equiv) was added dropwise at −78° C. The resulting yellow solution was stirred at −78° C. for 15 min, and at 0° C. for 30 min. Saturated aqueous NaHCO₃ was added. The mixture was stirred at rt for 10 min., and extracted with EtOAc three times. The combined organic layers were washed with brine, dried over sodium sulfate, and concentrated to provide 2.69 g of the phenol intermediate as an off-white solid. This material was used directly in the next step without further purification: ¹H NMR (400 MHz, CDCl₃) δ 7.46 (dd, J=7.8, 7.8 Hz, 2H), 7.32 (dd, J=7.8, 7.8 Hz, 1H), 7.27 (s, 1H), 7.19 (d, J=7.8 Hz, 2H), 2.83 (s, 3H); MS (ESI) m/z 339.0 (M−H), calcd for C₄H₉BrClO₃ 338.95.

The above phenol (2.65 g, 7.76 mmol, 1.0 equiv) was dissolved in acetone (40 mL), and added with K₂CO₃ (2.14 g, 15.5 mmol, 2.0 equiv) and benzylbromide (0.97 mL, 8.15 mmol, 1.05 equiv) at rt. After stirring overnight at rt, the solution was filtered through a bed of Celite. The solid cake was further washed with three portions of EtOAc. The combined organic solution was concentrated. The residue was purified by flash chromatography to yield 2.97 g (89%) of compound S15-7 as a white solid: ¹H NMR (400 MHz, CDCl₃) δ 7.33-7.43 (m, 7H), 7.19-7.26 (m, 2H), 7.05 (d, J=7.8 Hz, 2H), 5.11 (s, 2H), 2.51 (s, 3H); MS (ESI) m/z 429.0 (M−H), calcd for C₂₁H₁₅BrClO₃ 429.00.

To a solution of compound S15-7 (1.98 g, 4.59 mmol, 1.0 equiv) in anhydrous THF (23 mL) was added i-PrMgCl.LiCl (7.65 mL, 1.2 M/THF, 9.18 mmol, 2.0 equiv) dropwise at −78° C. under a N₂ atmosphere. After 10 min, the temperature was raised to 0° C. and the reaction was stirred for 1 h at 0° C. DMF (1.80 mL, 22.90 mmol, 5.0 equiv) was then added. The reaction was warmed to rt, stirred for 30 min at rt, and quenched by saturated aqueous NH₄Cl. The layers were separated and the aqueous layer was further extracted twice with EtOAc. The combined organic layers were washed with brine, dried over sodium sulfate, filtered, and concentrated. Purification of the residue by flash chromatography gave compound S15-8 (1.45 g, 83%) as a white solid: ¹H NMR (400 MHz, CDCl₃) δ 10.51 (s, 1H), 7.33-7.44 (m, 8H), 7.25-7.27 (m, 1H), 7.05 (d, J=7.8 Hz, 2H), 5.19 (s, 2H), 2.51 (s, 3H); MS (ESI) m/z 379.1 (M−H), calcd for C₂₂H₁₆ClO₄ 379.08.

To a solution of aldehyde S15-8 (1.66 g, 4.37 mmol, 1.0 equiv) in MeOH (22 mL) was added trimethylorthoformate (2.40 mL, 21.90 mmol, 5.0 equiv) and TsOH (83 mg, 0.44 mmol, 0.1 equiv). The reaction was heated to 65° C. for 4 hrs. The solvent was evaporated. The residue was redissolved in EtOAc (200 mL), washed with saturated aqueous NaHCO₃ and brine, dried over sodium sulfate, and concentrated. Purification of the residue by flash chromatography gave compound S15-9 (1.75 g, 94%) as a white solid: ¹H NMR (400 MHz, CDCl₃) δ 7.43 (d, J=7.8 Hz, 2H), 7.34-7.37 (m, 5H), 7.20-7.25 (m, 2H), 7.07 (d, J=7.8 Hz, 2H), 5.62 (s, 1H), 5.19 (s, 2H), 3.36 (s, 6H), 2.48 (s, 3H); MS (ESI) m/z 425.10 (M−H), calcd for C₂₄H₂₂ClO₅ 425.12.

A solution of n-BuLi in hexanes (2.37 mL, 2.2 M, 5.23 mmol, 1.2 equiv) was added dropwise to a solution of i-Pr₂NH (0.77 mL, 5.45 mmol, 1.25 equiv) in THF (27 mL) at −78° C. under a N₂ atmosphere. The resulting solution was stirred at −78° C. for 20 min and 0° C. for 5 min, and then re-cooled to −78° C. N,N,N′,N′-Tetramethylethylenediamine (TMEDA, 0.85 mL, 5.67 mmol, 1.30 equiv) was added, followed by the dropwise addition of S15-9 (2.05 g, 4.80 mmol, 1.1 equiv) in THF (30 mL) via a cannula. After complete addition, the resulting dark-red mixture was stirred for another hour at −78° C. and then cooled to −100° C. A solution of enone 51-9 (2.10 g, 4.36 mmol, 1.0 equiv) in THF (30 mL) was added dropwise via a cannula. The resulting red mixture was slowly warmed to −78° C. LHMDS (4.36 mL, 1.0 M/THF, 4.36 mmol, 1.0 equiv) was then added and the reaction was slowly warmed to −5° C. Saturated aqueous NH₄Cl was added. The resulting mixture was extracted three times with EtOAc. The combined EtOAc extracts were washed with brine, dried (sodium sulfate), and concentrated. Purification of the residue by flash chromatography gave compound S15-10 (3.20 g, 90%) as a light yellow foam: ¹H NMR (400 MHz, CDCl₃) δ 0.16 (s, 3H), 7.22-7.52 (m, 11H), 5.55 (s, 1H), 5.38 (s, 2H), 5.29 (d, J=11.4 Hz, 1H), 5.24 (d, J=11.4 Hz, 1H), 3.97 (d, J=10.4 Hz, 1H), 3.46 (dd, J=4.9, 15.9 Hz, 1H), 3.38 (s, 3H), 3.29 (s, 3H), 2.96-3.04 (m, 1H), 2.45-2.58 (m, 9H), 2.15 (d, J=14.6 Hz, 1H), 0.84 (s, 9H), 0.28 (s, 3H); MS (ESI) m/z 815.30 (M+H), calcd for C₄₄H₅₂ClN₂O₉Si 815.31.

To a solution of compound S15-10 (1.48 g, 1.82 mmol, 1.0 equiv) in THF (15 mL) was added 3 N HCl (3 mL) at 0° C. The resulting mixture was stirred at rt for 4 hrs, diluted with EtOAc, washed with saturated aqueous NaHCO₃ and brine, dried over sodium sulfate, and concentrated. Purification of the residue by flash chromatography gave aldehyde S15-11 (1.05 g, 75%) as a light yellow foam: ¹H NMR (400 MHz, CDCl₃) δ 15.8 (s, 1H), 10.5 (s, 1H), 7.28-7.50 (m, 11H), 5.36 (s, 2H), 5.28 (d, J=11.4 Hz, 1H), 5.23 (d, J=11.4 Hz, 1H), 3.94 (d, J=10.4 Hz, 1H), 3.48 (dd, J=4.9, 15.9 Hz, 1H), 2.96-3.06 (m, 1H), 2.44-2.60 (m, 9H), 2.16 (d, J=14.6 Hz, 1H), 0.82 (s, 9H), 0.26 (s, 3H), 0.14 (s, 3H); MS (ESI) m/z 769.30 (M+H), calcd for C₄₂H₄₆ClN₂O₈Si 769.26.

Compound S15-11 (30 mg, 0.039 mmol, 1.0 equiv) was dissolved in 1,2-dichloroethane (1.0 mL). Isobutylamine (14 μL, 0.12 mmol, 3.0 equiv) and acetic acid (7 μL, 0.12 mmol, 3.0 equiv) were added. After stirring at rt for 1 h, sodium triacetoxyborohydride (17 mg, 0.078 mmol, 2.0 equiv) was added. Stirring was continued overnight. The reaction mixture was poured into saturated aqueous NaHCO₃ and extracted three times with dichloromethane. The combined organic extracts were washed with brine, dried over sodium sulfate, and concentrated to give the crude intermediate, which was used directly in the next step without further purification.

In a plastic vial, the above amine intermediate was dissolved in CH₃CN (1 mL). Aqueous HF (48-500%, 0.25 mL) was added. After stirring at rt for 16 hrs, the reaction mixture was poured into aqueous solution (12.5 mL) of K₂HPO₄ (1.75 g) and extracted three times with dichloromethane. The combined organic phases were washed with brine, dried, and concentrated to yield the crude intermediate.

The above crude intermediate was dissolved in EtOAc (2.0 mL). Pd—C (10 wt %, 8 mg, 300 w/w) was added. The reaction flask was briefly evacuated and re-filled with hydrogen. The reaction mixture was stirred at rt and monitored by LC-MS. After the reaction was complete, MeOH (5 mL) and 0.5 N HCl/MeOH (0.5 mL) were added. The mixture was stirred for 30 min, and filtered through a small pad of Celite. The filtrate was concentrated to give the crude product, which was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 20→100% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 7.85-8.95 min, were collected and freeze-dried to give the desired product S15-13-1 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.16 (s, 1H), 7.16 (s, 1H), 4.38 (s, 2H), 4.12 (s, 1H), 3.40 (dd, J=4.6, 16.0 Hz, 1H), 2.96-3.11 (m, 10H), 2.41 (dd, J=14.0, 14.0 Hz, 1H), 2.24-2.28 (m, 1H), 2.08-2.13 (m, 1H), 1.60-1.70 (m, 1H), 1.06 (d, J=6.4 Hz, 6H); MS (ESI) m/z 534.2 (M+H), calcd for C₂₆H₃₃ClN₃O₇ 534.19.

The following compounds were prepared similarly to S15-13-1.

The crude product was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 20→100% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 8.00-9.50 min, were collected and freeze-dried to give the desired product S15-13-2 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.33 (s, 2H), 4.13 (s, 1H), 3.41 (dd, J=4.6, 16.0 Hz, 1H), 2.96-3.10 (m, 8H), 2.40 (dd, J=15.0, 15.0 Hz, 1H), 2.25-2.28 (m, 1H), 1.84 (q, J=7.3 Hz, 2H), 1.60-1.70 (m, 1H), 1.44 (s, 6H), 1.04 (t, J=7.3 Hz, 3H); MS (ESI) m/z 548.3 (M+H), calcd for C₂₇H₃₅ClN₃O₇ 548.21.

The crude product was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 20→100% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 8.35-9.80 min, were collected and freeze-dried to give the desired product S15-13-3 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.44 (s, 2H), 4.13 (s, 1H), 3.41 (dd, J=4.6, 16.0 Hz, 1H), 2.96-3.10 (m, 8H), 2.41 (dd, J=15.0, 15.0 Hz, 1H), 2.23-2.28 (m, 1H), 1.60-1.70 (m, 1H), 1.34 (s, 6H), 1.21-1.26 (m, 1H), 0.69-0.72 (m, 2H), 0.60-0.63 (m, 2H); MS (ESI) m/z 560.3 (M+H), calcd for C₂₈H₃₅ClN₃O₇ 560.21.

The crude product was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 20→100% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 7.15-8.50 min, were collected and freeze-dried to give the desired product S15-13-4 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.72 (t, J=13.2 Hz, 1H), 4.10-4.15 (m, 2H), 3.38-3.55 (m, 2H), 2.95-3.10 (m, 8H), 2.41 (dd, J=15.0, 15.0 Hz, 1H), 1.96-2.28 (m, 5H), 1.96-2.28 (m, 5H), 1.66 (s, 3H), 1.63-1.66 (m, 1H), 1.45 (s, 3H); MS (ESI) m/z 560.3 (M+H), calcd for C₂₈H₃₅ClN₃O₇ 560.21.

The crude product was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 20→100% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 7.65-8.50 min, were collected and freeze-dried to give the desired product S15-13-5 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.20 (s, 1H), 4.24 (s, 2H), 4.13 (s, 1H), 3.41 (dd, J=4.6, 16.0 Hz, 1H), 2.94-3.14 (m, 10H), 2.41 (dd, J=15.0, 15.0 Hz, 1H), 2.24-2.30 (m, 1H), 1.60-1.70 (m, 1H), 1.08 (s, 9H); MS (ESI) m/z 548.3 (M+H), calcd for C₂₇H₃₅ClN₃O₇ 548.21.

The crude product was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 0→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 13.80-14.90 min, were collected and freeze-dried to give the desired product S15-13-6 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.14 (s, 1H), 4.40 (s, 2H), 4.13 (s, 1H), 3.40 (dd, J=4.6, 16.0 Hz, 1H), 2.94-3.14 (m, 10H), 2.40 (dd, J=15.0, 15.0 Hz, 1H), 2.25-2.28 (m, 1H), 1.60-1.70 (m, 1H), 1.16-1.21 (m, 1H), 0.72-0.77 (m, 2H), 0.43-0.47 (m, 2H); MS (ESI) m/z 532.2 (M+H), calcd for C₂₆H₃₁ClN₃O₇ 532.18.

The crude product was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 0→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 15.55-16.00 min, were collected and freeze-dried to give the desired product S15-13-7 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.15 (s, 1H), 4.37 (s, 2H), 4.12 (s, 1H), 3.40 (dd, J=4.6, 16.0 Hz, 1H), 2.94-3.14 (m, 10H), 2.40 (dd, J=15.0, 15.0 Hz, 1H), 2.24-2.28 (m, 1H), 1.60-1.88 (m, 7H), 1.21-1.35 (m, 3H), 1.01-1.09 (m, 2H); MS (ESI) m/z 574.3 (M+H), calcd for C₂₉H₃₇ClN₃O₇ 574.22.

The crude product was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 0→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 13.85-14.90 min, were collected and freeze-dried to give the desired product S15-13-8 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.18 (s, 1H), 4.40 (s, 2H), 4.13 (s, 1H), 3.41 (dd, J=4.6, 16.0 Hz, 1H), 3.32-3.36 (m, 1H), 2.94-3.11 (m, 8H), 2.40 (dd, J=15.0, 15.0 Hz, 1H), 2.20-2.28 (m, 2H), 1.60-1.70 (m, 1H), 1.34 (d, J=6.8 Hz, 3H), 1.06 (d, J=6.8 Hz, 3H), 1.01 (d, J=6.8 Hz, 3H); MS (ESI) m/z 548.2 (M+H), calcd for C₂₇H₃₅ClN₃O₇ 548.21.

The crude product was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 20→100% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 9.60-10.85 min, were collected and freeze-dried to give the desired product S15-13-9 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.20 (s, 1H), 4.41 (s, 2H), 4.15 (s, 1H), 3.40 (dd, J=4.6, 16.0 Hz, 1H), 2.83-3.15 (m, 10H), 2.39 (dd, J=15.0, 15.0 Hz, 1H), 2.26-2.32 (m, 1H), 2.03 (br. s, 3H), 1.59-1.81 (m, 13H); MS (ESI) m/z 626.3 (M+H), calcd for C₃₃H₄₁ClN₃O₇ 626.26.

The crude product was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 10→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 15.15-16.45 min, were collected and freeze-dried to give the desired product S15-13-10 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.17 (s, 1H), 4.34 (s, 2H), 4.13 (s, 1H), 3.41 (dd, J=4.6, 16.0 Hz, 1H), 2.94-3.15 (m, 8H), 2.40 (dd, J=15.0, 15.0 Hz, 1H), 2.24-2.28 (m, 1H), 1.83 (s, 2H), 1.60-1.70 (m, 1H), 1.58 (s, 6H), 1.11 (s, 9H); MS (ESI) m/z 590.3 (M+H), calcd for C₃₀H₄₁ClN₃O₇ 590.26.

To a solution of compound S15-13-5 (12 mg, 0.022 mmol, 1.0 equiv) in DMF (0.5 mL) was added sequentially triethylamine (6 μL, 0.045 mmol, 2.0 equiv), HCHO (37 wt % in H₂O, 5 μL, 0.067 mmol, 3.0 equiv) and InCl₃ (0.5 mg, 0.0022 mmol, 0.1 equiv) at rt. After stirring for 5 min, Na(OAc)₃BH (10 mg, 0.045 mmol, 2.0 equiv) was added. Stirring was continued for another hour. HCl/MeOH (0.20 mL, 0.5 N) was added. The resulting mixture was added dropwise to vigorously stirring diethyl ether (100 mL). The precipitate was collected onto a small Celite pad and washed three times with more diethyl ether. The Celite pad was then eluted several times with MeOH. The MeOH solution was collected and concentrated to provide the crude product, which was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 0→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 13.90-15.40 min, were collected and freeze-dried to give the desired product S15-13-11 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.28 (s, 1H), 4.71-4.78 (m, 1H), 4.43-4.48 (m, 1H), 4.13 (s, 1H), 3.41 (dd, J=4.6, 16.0 Hz, 1H), 2.94-3.15 (m, 13H), 2.44 (dd, J=15.0, 15.0 Hz, 1H), 2.24-2.28 (m, 1H), 1.60-1.70 (m, 1H), 1.09 (s, 9H); MS (ESI) m/z 562.2 (M+H), calcd for C₂₈H₃₇ClN₃O₇ 562.22.

Compound S15-13-12 was prepared similarly from 515-13-8. The crude product was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 0→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 14.15-15.25 min, were collected and freeze-dried to give the desired product S15-13-12 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.26-4.76 (m, 2H), 4.12 (s, 1H), 3.34-3.44 (m, 2H), 2.82-3.11 (m, 11H), 2.18-2.47 (m, 3H), 1.60-1.70 (m, 1H), 1.39-1.46 (m, 3H), 1.03-1.08 (m, 6H); MS (ESI) m/z 562.2 (M+H), calcd for C₂₈H₃₇ClN₃O₇ 562.22.

Compound S15-13-13 was prepared similarly from S15-13-9. The crude product was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 20→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 17.00-18.85 min, were collected and freeze-dried to give the desired product S15-13-13 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.68-4.74 (m, 1H), 4.43-4.47 (m, 1H), 4.12 (s, 1H), 3.42 (dd, J=4.6, 16.0 Hz, 1H), 2.94-3.12 (m, 13H), 2.45 (dd, J=15.0, 15.0 Hz, 1H), 2.24-2.29 (m, 1H), 2.02 (br. s, 3H), 1.57-1.81 (m, 13H); MS (ESI) m/z 640.2 (M+H), calcd for C₃₄H₄₃ClN₃O₇ 640.27.

Compound S15-13-14 was prepared similarly from S15-13-10. The crude product was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 10→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 16.35-17.65 min, were collected and freeze-dried to give the desired product S15-13-14 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.17 (s, 1H), 4.12-4.18 (m, 1H), 4.11 (s, 1H), 3.40-3.50 (m, 2H), 2.94-3.15 (m, 8H), 2.75 (s, 3H), 2.39-2.48 (m, 1H), 2.24-2.28 (m, 1H), 1.83-2.00 (m, 2H), 1.73 (s, 3H), 1.60-1.70 (m, 1H), 1.63 (s, 3H), 1.14 (s, 9H); MS (ESI) m/z 604.3 (M+H), calcd for C₃₁H₄₃ClN₃O₇ 604.27.

To a solution of compound S15-8 (0.39 g, 1.02 mmol, 1.0 equiv) in 1,2-dichloroethane (10 mL) was added t-butylamine (0.13 mL, 1.22 mmol, 1.2 equiv) and acetic acid (0.18 mL, 3.05 mmol, 3.0 equiv). After stirring at rt for 2.5 hrs, sodium triacetoxyborohydride (0.43 g, 2.03 mmol, 2.0 equiv) was added. Stirring was continued overnight. The reaction mixture was poured into saturated aqueous NaHCO₃ and extracted three times with dichloromethane. The combined organic extracts were washed with brine, dried over sodium sulfate, and concentrated to give the crude intermediate as an off-white oil. The crude intermediate was redissolved in 1,2-dichloroethane (10 mL). Formaldehyde (37 wt % in H₂O, 0.76 mL, 10.20 mmol, 10 equiv) and acetic acid (0.18 mL, 3.05 mmol, 3.0 equiv) were added After stirring at rt for 1 h, sodium triacetoxyborohydride (0.43 g, 2.03 mmol, 2.0 equiv) was added. Stirring was continued for another hour. The reaction mixture was poured into saturated aqueous NaHCO₃ and extracted three times with dichloromethane. The combined organic extracts were washed with brine, dried over sodium sulfate, and concentrated. Purification of the residue by flash chromatography gave compound S15-15-1 (0.35 g) as a white solid: ¹H NMR (400 MHz, CDCl₃) δ 7.43 (d, J=7.8 Hz, 2H), 7.20-7.40 (m, 7H), 7.12 (d, J=7.8 Hz, 2H), 5.18 (s, 2H), 3.59 (s, 2H), 2.44 (s, 3H), 2.09 (s, 3H), 1.10 (s, 9H); MS (ESI) m/z 452.3 (M+H), calcd for C₂₇H₃₁ClNO₃ 452.19.

Compound S15-12-1 was prepared by the procedure of S15-10 employing compound S15-15-1: ¹H NMR (400 MHz, CDCl₃) δ 16.0 (s, 1H), 7.22-7.52 (m, 11H), 5.36 (s, 2H), 5.27 (s, 2H), 3.97 (d, J=10.4 Hz, 1H), 3.55 (s, 2H), 3.42 (dd, J=4.9, 15.9 Hz, 1H), 2.93-3.04 (m, 1H), 2.43-2.53 (m, 9H), 2.13 (d, J=14.6 Hz, 1H), 2.01 (s, 3H), 1.07 (s, 9H), 0.84 (s, 9H), 0.28 (s, 3H), 0.13 (s, 3H); MS (ESI) m/z 840.4 (M+H), calcd for C₄₇H₅₉ClN₃O₇Si 840.37.

In a plastic vial, compound S15-12-1 (85 mg, 0.10 mmol) was dissolved in CH₃CN (2 mL). Aqueous HF (48-50%, 0.5 mL) was added. After stirring at rt for 7 hrs, the reaction mixture was poured into an aqueous solution (25 mL) of K₂HPO₄ (3.5 g). The resulting mixture was extracted three times with dichloromethane. The combined organic phases were washed with brine, dried, and concentrated under reduced pressure to yield the crude desilylated product.

The above crude intermediate was dissolved in EtOAc (6 mL). Pd—C (10 wt %, 22 mg) was added. The reaction flask was briefly evacuated and re-filled with hydrogen. The reaction mixture was stirred at rt and monitored by LC-MS. After the reaction was complete, MeOH (5 mL) and 0.5 N HCl/MeOH (0.5 mL) were added. The mixture was stirred for 30 min and filtered through a small pad of Celite. The filtrate was concentrated to give the crude product, which was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 20→100% B over 10 min: mass-directed fraction collection]. Fractions containing the desired product, eluting at 6.30-8.85 min, were collected and freeze-dried to give the desired product S15-13-15 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.20 (s, 1H), 4.80-4.87 (m, 1H), 4.12-4.20 (m, 1H), 4.12 (s, 1H), 3.38-3.40 (m, 1H), 2.96-3.11 (m, 8H), 2.75 (s, 3H), 2.38-2.40 (m, 1H), 2.24-2.28 (m, 1H), 1.60-1.70 (m, 1H), 1.57 (s, 9H); MS (ESI) m/z 548.2 (M+H), calcd for C₂₇H₃₅ClN₃O₇ 548.21.

Alternatively, the crude desilylated product of S15-12-1 (13 mg) was dissolved in 0.5 N HCl/MeOH (0.07 mL). The volatiles were evaporated. The residue was redissolved in MeOH (2 mL) and added with Pd—C (10 wt %, 7 mg). The reaction flask was briefly evacuated and re-filled with hydrogen. The reaction mixture was stirred at rt and monitored by LC-MS. After all the reaction was complete, the reaction mixture was filtered through a small pad of Celite. The filtrate was concentrated to yield the crude product, which was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 10→150% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 6.08-8.75 min, were collected and freeze-dried to give the following two products.

S15-14-1, a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 6.93 (s, 1H), 4.65 (d, J=12.8 Hz, 1H), 4.08 (s, 1H), 3.90 (d, J=12.8 Hz, 1H), 2.96-3.11 (m, 9H), 2.70 (s, 3H), 2.58-2.68 (m, 1H), 2.19-2.22 (m, 1H), 1.60-1.70 (m, 1H), 1.53 (s, 9H); MS (ESI) m/z 514.3 (M+H), calcd for C₂₇H₃₆N₃O₇ 514.25.

S15-14-2, a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 6.62 (s, 1H), 6.59 (s, 1H), 4.06 (s, 1H), 2.77-3.11 (m, 9H), 2.51 (dd, J=14.2, 14.2 Hz, 1H), 2.30 (s, 3H), 2.14-2.17 (m, 1H), 1.52-1.62 (m, 1H); MS (ESI) m/z 429.3 (M+H), calcd for C₂₂H₂₅N₂O₇ 429.16.

The following compounds were prepared using similar procedures to that of S15-13-1, S15-13-11, S15-13-15 or S15-14-1.

S15-13-16, a yellow solid:

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.53 (s, 2H), 4.13 (s, 1H), 3.30-3.45 (m, 5H), 2.96-3.15 (m, 8H), 2.40 (dd, J=15.9, 15.9 Hz, 1H), 2.25-2.29 (m, 1H), 1.61-1.79 (m, 5H), 1.09 (s, 3H), 1.03 (s, 3H); MS (ESI) m/z 574.3 (M+H), calcd for C₂₉H₃₇ClN₃O₇ 574.22.

S15-14-3, a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 6.95 (s, 1H), 4.28 (s, 2H), 4.09 (s, 1H), 2.96-3.15 (m, 13H), 2.61 (dd, J=15.9, 15.9 Hz, 1H), 2.18-2.22 (m, 1H), 1.59-1.75 (m, 5H), 1.09 (s, 3H), 1.04 (s, 3H); MS (ESI) m/z 540.3 (M+H), calcd for C₂₉H₃₈N₃O₇ 540.26.

S15-14-4, a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 6.97 (s, 1H), 6.90 (s, 1H), 4.18 (s, 2H), 4.09 (s, 1H), 2.94-3.14 (m, 11H), 2.60 (dd, J=15.0, 15.0 Hz, 1H), 2.19-2.22 (m, 1H), 1.59-1.65 (m, 1H), 1.10-1.15 (m, 1H), 0.71-0.74 (m, 2H), 0.40-0.43 (m, 2H); MS (ESI) m/z 497.2 (M+H), calcd for C₂₆H₃₂N₃O₇ 497.22.

¹H NMR (400 MHz, CD₃OD) δ 7.31 (s, 1H), 4.56 (dd, J=19.2 Hz, 13.6 Hz, 2H), 4.15 (s, 1H), 4.08-3.98 (m, 2H), 3.90-3.79 (m, 2H), 3.53-3.33 (m, 5H), 3.20-2.90 (m, 8H), 2.45-2.36 (m, 1H), 2.32-2.25 (m, 1H), 1.71-1.60 (m, 1H); MS (ESI) m/z 548.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.16 (s, 1H), 4.40 (s, 2H), 4.14 (s, 1H), 3.50-3.36 (m, 1H), 3.32-3.21 (m, 1H), 3.18-2.90 (m, 8H), 2.48-2.36 (m, 1H), 2.31-2.20 (m, 3H), 1.98-1.88 (m, 2H), 1.80-1.60 (m, 2H), 1.50-1.35 (m, 4H), 1.34-1.20 (m, 1H); MS (ESI) m/z 560.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.13 (s, 1H), 4.26 (s, 2H), 4.13 (s, 1H), 3.95-3.86 (m, 1H), 3.45-3.37 (m, 1H), 3.18-2.95 (m, 8H), 2.45-2.33 (m, 3H), 2.32-2.20 (m, 3H), 2.02-1.90 (m, 2H), 1.72-1.60 (m, 1H); MS (ESI) m/z 532.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.16 (s, 1H), 4.38 (s, 2H), 4.13 (s, 1H), 3.79-3.65 (m, 1H), 3.45-3.38 (m, 1H), 3.17-2.95 (m, 8H), 2.48-2.38 (m, 1H), 2.30-2.15 (m, 3H), 1.91-1.78 (m, 2H), 1.80-1.67 (m, 5H); MS (ESI) m/z 546.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.72-4.56 (m, 2H), 4.50-4.32 (m, 1H), 4.14 (s, 1H), 3.97-3.78 (m, 1H), 3.75-3.50 (m, 2H), 3.46-3.38 (m, 1H), 3.20-2.90 (m, 9H), 2.70-2.52 (m, 1H), 2.50-2.38 (m, 1H), 2.32-2.24 (m, 1H), 2.20-2.05 (m, 1H), 1.97 (s, 3H), 1.73-1.61 (m, 1H); MS (ESI) m/z 589.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.72-4.56 (m, 2H), 4.48-4.33 (m, 1H), 4.14 (s, 1H), 3.98-3.79 (m, 1H), 3.75-3.50 (m, 2H), 3.45-3.38 (m, 1H), 3.18-2.90 (m, 9H), 2.70-2.51 (m, 1H), 2.50-2.38 (m, 1H), 2.32-2.24 (m, 1H), 2.20-2.05 (m, 1H), 1.97 (s, 3H), 1.73-1.61 (m, 1H); MS (ESI) m/z 589.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.20 (s, 1H), 4.42-4.32 (m, 2H), 4.16 (s, 1H), 3.71-3.60 (m, 1H), 3.46-3.41 (m, 1H), 3.20-2.95 (m, 8H), 2.71 (br s, 1H), 2.46-2.25 (m, 4H), 2.20-2.18 (m, 1H), 1.75-1.52 (m, 4H), 1.60-1.50 (m, 2H), 1.50-1.41 (m, 1H), 1.24-1.16 (m, 1H); MS (ESI) m/z 572.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.64 (s, 2H), 4.14 (s, 1H), 3.48-3.39 (m, 1H), 3.10-2.95 (m, 8H), 2.48-2.38 (m, 1H), 2.32-2.25 (m, 1H), 1.68-1.59 (m, 1H), 1.50-1.39 (m, 1H), 1.05-0.97 (m, 2H), 0.88-0.82 (m, 2H), 0.78-0.73 (m, 2H), 0.45-0.38 (m, 2H); MS (ESI) m/z 558.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.27 (s, 1H), 4.51 (s, 2H), 4.15 (s, 1H), 3.62-3.53 (m, 2H), 3.47-3.38 (m, 1H), 3.24-2.90 (m, 10H), 2.49-2.37 (m, 1H), 2.32-2.25 (m, 1H), 2.02-1.93 (m, 2H), 1.70-1.58 (m, 2H), 1.57-1.24 (m, 6H), 1.00-0.89 (m, 3H); MS (ESI) m/z 588.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.49 (s, 2H), 4.13 (s, 1H), 3.65-3.58 (m, 2H), 3.49-3.38 (m, 1H), 3.20-2.95 (m, 10H), 2.49-2.38 (m, 1H), 2.31-2.24 (m, 1H), 2.02-1.93 (m, 2H), 1.71-1.55 (m, 3H), 1.48-1.38 (m, 1H), 0.91 (s, 9H); MS (ESI) m/z 602.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.27 (s, 1H), 4.51 (s, 2H), 4.15 (s, 1H), 3.65-3.56 (m, 2H), 3.48-3.38 (m, 1H), 3.23-2.95 (m, 10H), 2.48-3.38 (m, 1H), 2.32-2.26 (m, 1H), 2.02-1.93 (m, 2H), 1.72-1.38 (m, 5H), 0.95 (d, J=6.4 Hz, 6H); MS (ESI) m/z 588.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.47-7.20 (m, 6H), 4.58 (s, 2H), 4.14 (s, 1H), 3.75-3.65 (m, 1H), 3.57-3.35 (m, 2H), 3.20-2.85 (m, 9H), 2.61-2.40 (m, 2H), 2.31-2.19 (m, 1H), 2.18-2.00 (m, 4H), 1.73-1.60 (m, 1H); MS (ESI) m/z 622.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.51 (s, 2H), 4.14 (s, 1H), 3.64-3.57 (m, 2H), 3.46-3.37 (m, 1H), 3.32 (s, 3H), 3.30-2.25 (m, 2H), 3.25-2.95 (m, 10H), 2.48-2.38 (m, 1H), 2.31-2.24 (m, 1H), 2.02-1.98 (m, 3H), 1.71-1.52 (m, 3H); MS (ESI) m/z 590.0 (M+H).

MS (ESI) m/z 588.1.4 (M+H), calcd for C₃₀H₃₉ClN₃O₉ 588.24.

¹H NMR (400 MHz, CD₃OD) δ 7.30 (s, 1H), 4.53 (dd, J=18.4 Hz, 13.6 Hz, 2H), 4.15 (s, 1H), 3.48-3.38 (m, 3H), 3.20-2.95 (m, 8H), 2.80-2.70 (m, 2H), 2.48-2.38 (m, 1H), 2.34-2.26 (m, 1H), 2.10-1.95 (m, 2H), 1.91-1.82 (m, 1H), 1.72-1.61 (m, 1H), 1.00 (d, J=6.4 Hz, 6H); MS (ESI) m/z 574.5 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.32 (s, 1H), 4.60-4.32 (m, 2H), 4.15 (s, 1H), 3.50-3.38 (m, 2H), 3.20-2.94 (m, 9H), 2.83-2.70 (m, 1H), 2.48-2.38 (m, 1H), 2.35-2.10 (m, 3H), 2.08-1.95 (m, 1H), 1.72-1.53 (m, 2H), 1.50-1.40 (m, 1H), 1.19 (d, J=6.8 Hz, 3H), 0.99 (d, J=6.8 Hz, 3H); MS (ESI) m/z 574.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.56-4.48 (m, 2H), 4.13 (s, 1H), 2.43-3.39 (m, 1H), 3.20-2.95 (m, 12H), 2.45-2.38 (m, 1H), 2.29-2.26 (m, 1H), 1.80-1.59 (m, 8H), 1.42-1.12 (m, 7H), 1.08-0.93 (m, 2H); MS (ESI) m/z 616.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.06 (s, 1H), 4.30 (s, 2H), 4.02 (s, 1H), 3.35-3.28 (m, 1H), 3.08-2.85 (m, 10H), 2.38-2.28 (m, 1H), 2.21-2.10 (m, 2H), 1.90-1.78 (m, 2H), 1.68-1.49 (m, 5H), 1.28-1.12 (m, 2H); MS (ESI) m/z 560.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.17 (s, 1H), 4.38 (s, 2H), 4.13 (s, 1H), 3.44-3.30 (m, 2H), 3.18-2.88 (m, 8H), 2.45-2.35 (m, 1H), 2.31-2.24 (m, 1H), 2.03-1.90 (m, 1H), 1.70-1.58 (m, 2H), 1.43 (d, J=7.0 Hz, 3H), 1.05 (t, J=7.2 Hz, 3H); MS (ESI) m/z 534.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.16 (s, 1H), 4.40 (s, 2H), 4.11 (s, 1H), 3.92-3.86 (m, 2H), 3.80-3.70 (m, 1H), 3.57-3.54 (m, 1H), 3.42-3.34 (m, 1H), 3.22-3.19 (m, 1H), 3.15-2.85 (m, 8H), 2.72-2.60 (m, 1H), 2.43-2.35 (m, 1H), 2.28-2.13 (m, 2H), 1.76-1.58 (m, 2H); MS (ESI) m/z 562.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.20 (s, 1H), 4.57 (s, 2H), 4.10 (s, 1H), 3.97 (dd, J=12.0 Hz and 8.0 Hz, 1H), 3.86 (dd, J=12.0 Hz and 7.6 Hz, 1H), 3.41 (dd, J=16.4 Hz and 4.8 Hz, 1H), 3.13-2.94 (m, 9H), 2.44-2.37 (m, 1H), 2.28-2.20 (m, 1H), 1.69-1.59 (m, 1H), 1.08 (s, 9H); MS (ESI) m/z 578.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.44 (s, 2H), 4.14 (s, 1H), 3.42 (dd, J=12.0 Hz and 4.8 Hz, 1H), 3.22-2.87 (m, 10H), 2.48-2.38 (m, 1H), 2.31-2.25 (m, 1H), 2.00-1.80 (m, 6H), 1.70-1.58 (m, 3H), 0.89 (t, J=7.6 Hz, 3H); MS (ESI) m/z 574.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.75-7.65 (m, 2H), 7.60-7.48 (m, 3H), 6.97 (s, 1H), 4.12 (s, 1H), 4.00 (s, 2H), 3.37-3.32 (m, 1H), 3.10-2.95 (m, 8H), 2.41-2.32 (m, 1H), 2.30-2.20 (m, 1H), 1.89 (s, 6H), 1.70-1.59 (m, 1H); MS (ESI) m/z 596.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.15 (s, 1H), 4.38 (s, 2H), 4.14 (s, 1H), 3.42 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.23-2.96 (m, 10H), 2.47-2.38 (m, 1H), 2.32-2.25 (m, 1H), 1.81-1.60 (m, 8H), 1.45-1.17 (m, 4H), 1.08-0.96 (m, 2H); MS (ESI) m/z 588.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.50 (s, 2H), 4.14 (s, 1H), 3.90-3.65 (m, 6H), 3.43 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.20-2.90 (m, 10H), 2.46-2.35 (m, 1H), 2.31-2.22 (m, 1H), 2.20-2.00 (m, 4H), 1.71-1.60 (m, 1H); MS (ESI) m/z 575.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.17 (s, 1H), 4.35 (s, 2H), 4.11 (s, 1H), 3.41 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.15-2.95 (m, 8H), 2.46-2.38 (m, 1H), 2.28-2.22 (m, 1H), 2.00-1.93 (m, 2H), 1.83-1.62 (m, 6H), 1.61-1.49 (m, 6H); MS (ESI) m/z 574.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.16 (s, 1H), 4.22 (s, 2H), 4.14 (s, 1H), 3.41 (dd, J=16.4 Hz and 4.4 Hz, 1H), 3.18-2.32 (m, 8H), 2.55-2.35 (m, 3H), 2.31-2.22 (m, 1H), 2.18-2.08 (m, 2H), 2.05-1.93 (m, 2H), 1.66 (s, 3H); MS (ESI) m/z 546.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.27 (s, 1H), 4.55-4.47 (m, 2H), 4.13 (s, 1H), 3.86 (m, 1H), 3.61-3.52 (m, 1H), 3.50-3.35 (m, 3H), 3.30-2.94 (m, 9H), 2.45-2.35 (m, 1H), 2.31-2.22 (m, 1H), 2.18-1.98 (m, 2H), 1.95-1.87 (m, 1H), 1.85-1.70 (m, 1H), 1.70-1.56 (m, 1H); MS (ESI) m/z 562.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.32-7.23 (m, 5H), 2.48-2.38 (m, 2H), 4.13 (s, 1H), 3.65-3.38 (m, 6H), 3.20-2.95 (m, 8H), 2.84 (s, 3H), 2.50-2.38 (m, 1H), 2.32-2.25 (m, 1H), 1.72-1.60 (m, 1H); MS (ESI) m/z 608.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.20-7.35 (m, 5H), 4.65-4.40 (m, 3H), 4.14 (s, 1H), 3.60-3.38 (m, 6H), 3.20-2.95 (m, 9H), 2.50-2.39 (m, 1H), 2.33-2.26 (m, 1H), 1.73-1.61 (m, 1H), 1.43 (t, J=7.6 Hz, 3H); MS (ESI) m/z 622.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.74 (t, J=13.6 Hz, 1H), 4.32 (t, J=11.6 Hz, 1H), 4.16 (s, 1H), 3.43 (dd, J=16.0 Hz and 3.6 Hz, 1H), 3.26-2.85 (m, 13H), 2.47-2.40 (m, 1H), 2.32-2.90 (m, 1H), 2.20-2.08 (m, 1H), 1.95-1.50 (m, 11H), 1.40-1.25 (m, 2H); MS (ESI) m/z 602.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.64 (t, J=13.6 Hz, 1H), 4.49 (t, J=11.6 Hz, 1H), 4.15 (s, 1H), 3.44 (dd, J=16.0 Hz and 3.6 Hz, 1H), 3.22-2.98 (m, 12H), 2.50-2.40 (m, 1H), 2.34-2.26 (m, 1H), 2.10-2.00 (m, 1H), 1.91-1.80 (m, 1H), 1.80-1.50 (m, 9H), 1.44 (t, J=7.6 Hz, 3H), 1.40-1.23 (m, 3H); MS (ESI) m/z 616.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.79 (d, J=13.6 Hz, 1H), 4.35 (d, J=13.2 Hz, 1H), 4.13 (s, 1H), 3.60-3.37 (m, 4H), 3.16-2.94 (m, 8H), 2.48-2.34 (m, 2H), 2.30-2.22 (m, 1H), 2.20-2.08 (m, 2H), 2.08-1.98 (m, 1H), 1.85-1.71 (m, 1H), 1.70-1.57 (m, 2H), 1.06 (t, J=7.6 Hz, 3H); MS (ESI) m/z 560.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.68 (t, J=13.6 Hz, 1H), 4.39 (t, J=14.0 Hz, 1H), 4.15 (s, 1H), 3.43-3.36 (m, 1H), 3.18-2.72 (m, 13H), 2.46-2.38 (m, 1H), 2.30-2.25 (m, 1H), 1.80-1.60 (m, 4H), 0.99 (d, J=7.6 Hz, 6H); MS (ESI) m/z 562.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.54 (s, 2H), 4.23 (s, 1H), 3.42 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.26-2.97 (m, 12H), 2.48-2.38 (m, 1H), 2.30-2.23 (m, 1H), 1.75-1.60 (m, 4H), 1.40 (t, J=7.6 Hz, 3H), 0.96 (t, J=7.2 Hz, 6H); MS (ESI) m/z 576.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.68 (t, J=13.6 Hz, 1H), 4.39 (t, J=14.0 Hz, 1H), 4.13 (s, 1H), 3.40 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.19-2.83 (m, 13H), 2.49-2.39 (m, 1H), 2.31-2.25 (m, 1H), 1.88-1.78 (m, 2H), 1.71-1.60 (m, 1H), 1.50-1.39 (m, 2H), 1.01 (t, J=7.6 Hz, 3H); MS (ESI) m/z 548.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.17 (s, 1H), 4.46 (s, 2H), 4.06 (s, 1H), 3.38-3.28 (m, 1H), 3.20-2.88 (m, 12H), 2.60-2.28 (m, 3H), 2.25-2.17 (m, 1H), 2.11-2.00 (m, 1H), 1.98-1.90 (m, 1H), 1.90-1.78 (m, 3H), 1.64-1.50 (m, 1H), 1.40-1.28 (m, 2H), 1.28-1.12 (m, 3H), 1.03-0.93 (m, 3H), 0.71 (s, 3H); MS (ESI) m/z 642.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.48 (d, J=13.6 Hz, 1H), 4.29 (d, J=13.6 Hz, 1H), 4.15 (s, 1H), 3.42 (dd, J=16.4 Hz and 4.4 Hz, 1H), 3.18-2.87 (m, 9H), 2.47-2.35 (m, 1H), 2.32-2.16 (m, 2H), 1.99-1.60 (m, 5H), 1.32-1.20 (m, 2H), 1.13 (s, 3H), 1.02 (s, 3H), 0.94 (s, 3H); MS (ESI) m/z 614.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.14 (s, 1H), 3.62-3.50 (m, 1H), 3.48-3.38 (m, 1H), 3.20-2.78 (m, 12H), 2.60-2.49 (m, 1H), 2.48-2.35 (m, 1H), 2.32-2.18 (m, 2H), 2.10-2.02 (m, 1H), 1.98-1.80 (m, 3H), 1.80-1.59 (m, 2H), 1.28 (s, 3H), 1.05 (s 3H), 0.95 (s, 3H); MS (ESI) m/z 628.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.55 (d, J=13.6 Hz, 1H), 4.29 (d, J=13.6 Hz, 1H), 4.14 (s, 1H), 3.71-3.52 (m, 2H), 3.50-3.38 (m, 2H), 3.25-2.94 (m, 9H), 2.58-2.37 (m, 2H), 2.38-2.24 (m, 2H), 2.09-1.82 (m, 4H), 1.80-1.62 (m, 2H), 1.42 (s, 3H), 1.28 (s, 3H), 1.07 (s, 3H), 0.96 (t, J=8.0 Hz, 3H); MS (ESI) m/z 642.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.16 (s, 1H), 4.38 (s, 2H), 4.13 (s, 1H), 3.41 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.15-2.97 (m, 10H), 2.44-2.37 (m, 1H), 2.30-2.22 (m, 1H), 2.04-1.92 (m, 1H), 1.88-1.78 (m, 2H), 1.78-1.48 (m, 9H), 1.39-1.27 (m, 2H); MS (ESI) m/z 588.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.68 (t, J=13.2 Hz, 1H), 4.29 (t, J=13.2 Hz, 1H), 4.16 (s, 1H), 3.80 (m, 1H), 3.44 (m, 1H), 3.20-2.90 (m, 8H), 2.81 (s, 3H), 2.50-2.38 (m, 1H), 2.34-2.26 (m, 1H), 1.73-1.60 (m, 1H), 1.52 (d, J=5.6 Hz, 3H), 1.47 (d, J=6.4 Hz, 1H); MS (ESI) m/z 534.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.65 (t, J=13.2 Hz, 1H), 4.39 (t, J=13.2 Hz, 1H), 4.16 (s, 1H), 3.91-3.82 (m, 1H), 3.45 (m, 1H), 3.28-2.90 (m, 10H), 2.51-2.40 (m, 1H), 2.34-2.26 (m, 1H), 1.75-1.62 (m, 1H), 1.61 (d, J=7.6 Hz, 3H), 1.45 (d, J=6.8 Hz, 3H), 1.37 (t, J=7.2 Hz, 3H); MS (ESI) m/z 547.9 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.74 (t, J=13.6 Hz, 1H), 4.29 (t, J=13.2 Hz, 1H), 4.16 (s, 1H), 3.50-3.39 (m, 2H), 3.20-2.97 (m, 8H), 2.82 (s, 3H), 2.49-2.38 (m, 1H), 2.34-2.26 (m, 1H), 2.20 (m, 2H), 2.00 (m, 2H), 1.80-1.55 (m, 4H), 1.52-1.38 (m, 2H), 1.35-1.25 (m, 1H); MS (ESI) m/z 574.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.64 (t, J=13.2 Hz, 1H), 4.51 (t, J=13.2 Hz, 1H), 4.16 (s, 1H), 3.45 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.22-2.90 (m, 12H), 2.51-2.42 (m, 1H), 2.35-2.26 (m, 1H), 1.96-1.64 (m, 7H), 1.43 (t, J=7.2 Hz, 3H), 1.40-1.20 (m, 3H), 1.15-0.95 (m, 2H); MS (ESI) m/z 601.9 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.40-7.26 (m, 6H), 4.72-4.58 (m, 2H), 4.16 (s, 1H), 3.52-2.39 (m, 5H), 3.21-2.88 (m, 10H), 2.50-2.39 (m, 1H), 2.35-2.26 (m, 1H), 1.74-1.62 (m, 1H), 1.46 (t, J=7.2 Hz, 3H); MS (ESI) m/z 610.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.51 (d, J=7.8 Hz, 2H), 7.41 (t, J=7.8 Hz, 2H), 7.30 (br s, 1H), 7.05 (s, 1H), 4.41 (t, J=12.4 Hz, 1H), 4.23 (t, J=12.4 Hz, 1H), 3.75 (dd, J=31.2 Hz and 13.6 Hz, 2H), 3.40-3.30 (m, 1H), 3.20-2.88 (m, 8H), 2.75 (s, 3H), 2.45-2.36 (m, 1H), 2.32-2.25 (m, 1H), 1.72-1.60 (m, 1H), 1.58 (s, 3H), 1.49 (s, 3H); MS (ESI) m/z 624.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.38 (m, 2H), 7.31-7.28 (m, 4H), 4.81 (t, J=14.0 Hz, 1H), 4.68 (t, J=14.0 Hz, 1H), 4.16 (s, 1H), 3.60-3.49 (m, 2H), 3.43 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.36-3.30 (m, 2H), 3.26-2.97 (m, 10H), 2.49-2.39 (m, 1H), 2.34-2.26 (m, 1H), 1.73-1.62 (m, 1H), 1.33-1.24 (m, 1H), 0.87-0.83 (m, 2H), 0.54-0.51 (m, 2H); MS (ESI) m/z 636.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.48-7.42 (m, 2H), 7.42-7.33 (m, 2H), 7.33-7.25 (m, 1H), 7.12-7.08 (m, 1H), 4.57-4.49 (m, 1H), 4.44-4.34 (m, 1H), 4.16 (s, 1H), 3.91-3.82 (m, 1H), 3.66-3.58 (m, 1H), 3.36-3.28 (m, 1H), 3.24-2.97 (m, 10H), 2.46-2.32 (m, 1H), 2.32-2.25 (m, 1H), 1.73-1.60 (m, 1H), 1.55 (s, 3H), 1.44 and 1.42 (each s, total 3H), 1.30-1.18 (m, 1H), 0.83-0.73 (m, 2H), 0.40-0.31 (m, 2H); MS (ESI) m/z 664.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.30 (s, 1H), 4.74-4.62 (m, 2H), 4.16 (s, 1H), 3.45 (dd, J=12.4 Hz and 4.4 Hz, 1H), 3.39-3.31 (m, 1H), 3.28-3.22 (m, 2H), 3.20-2.98 (m, 9H), 2.53-2.43 (m, 1H), 2.35-2.26 (m, 1H), 2.12-2.02 (m, 1H), 1.92-1.82 (m, 1H), 1.80-1.45 (m, 10H), 1.40-1.18 (m, 3H), 0.90-0.82 (m, 2H), 0.55-0.48 (m, 2H); MS (ESI) m/z 642.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.26 and 7.24 (each s, total 1H), 4.68-4.59 (m, 1H), 4.58-4.46 (m, 1H), 4.15 (s, 1H), 3.75-3.63 (m, 1H), 3.48-3.37 (m, 1H), 3.25-2.98 (m, 11H), 2.50-2.40 (m, 1H), 2.33-2.26 (m, 1H), 2.12-1.92 (m, 1H), 1.85-1.60 (m, 2H), 1.54 and 1.44 (each d, J=6.8 Hz, total 3H), 1.18-1.02 (m, 4H), 0.82-0.73 (m, 2H), 0.50-0.38 (m, 2H); MS (ESI) m/z 588.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.31 and 7.24 (each s, total 1H), 4.75-4.64 (m, 1H), 4.39-4.30 (m, 1H), 4.16 (s, 1H), 3.85-3.76 (m, 1H), 3.48-3.39 (m, 1H), 3.26-2.98 (m, 10H), 2.50-2.12 (m, 3H), 1.73-1.62 (m, 1H), 1.44 (d, J=6.4 Hz, 3H), 1.30-1.00 (m, 7H), 0.88-0.68 (m, 2H), 0.60-0.38 (m, 2H); MS (ESI) m/z 602.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.30 (s, 1H), 4.70-4.50 (m, 2H), 4.14 (s, 1H), 3.48-3.35 (m, 3H), 3.18-2.86 (m, 10H), 2.48-2.37 (m, 1H), 2.31-2.22 (m, 1H), 1.71-1.58 (m, 1) 1.47 (t, J=6.4 Hz, 3H), 1.06 (s, 9H); MS (ESI) m/z 576.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.34 (s, 1H), 4.77-4.66 (m, 2H), 4.14 (s, 1H), 3.49-3.38 (m, 2H), 3.32-3.18 (m, 1H), 3.18-2.94 (m, 10H), 2.50-2.37 (m, 1H), 2.30-2.22 (m, 1H), 1.71-1.60 (m, 1H), 1.38-1.28 (m, 1H), 1.03 (s, 9H), 0.90-0.74 (m, 2H), 0.55-0.44 (m, 2H); MS (ESI) m/z 602.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.50 and 4.38 (each d, J=13.6 Hz, total 1H), 4.16 (s, 1H), 3.52-3.35 (m, 4H), 3.20-2.98 (m, 9H), 2.50-2.40 (m, 1H), 2.34-2.26 (m, 1H), 1.75-1.62 (m, 1H), 1.58 and 1.53 (each d, J=6.4 Hz, total 3H), 1.38 and 1.29 (each t, J=7.2 Hz, total 3H), 0.96-0.78 (m, 2H), 0.72-0.40 (m, 2); MS (ESI) m/z 574.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.28 and 7.25 (each s, total 1H), 4.76-4.72 (m, 1H), 4.47-4.40 (m, 1H), 4.14 (s, 1H), 3.57-3.35 (m, 3H), 3.20-2.98 (m, 10H), 2.50-2.40 (m, 1H), 2.33-2.26 (m, 1H), 1.74-1.62 (m, 1H), 1.51 (t, J=7.2 Hz, 3H), 1.45-1.22 (m, 1H), 1.22-1.16 and 1.05-0.95 (each m, total 1H), 0.90-0.60 (m, 5H), 0.60-0.34 (m, 3H); MS (ESI) m/z 600.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.15 (s, 1H), 6.36 (tt, J=53.6 Hz and 3.2 Hz, 1H), 4.50 (s, 2H), 4.12 (s, 1H), 3.71 (dt, J=15.2 Hz and 3.2 Hz, 2H), 3.41 (dd, J=16.0 Hz and 4.8 Hz, 1H), 3.15-2.95 (m, 8H), 2.46-2.38 (m, 1H), 2.30-2.22 (m, 1H), 1.72-1.61 (m, 1H); MS (ESI) m/z 542.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 6.50 (tt, J=53.6 Hz and 3.2 Hz, 1H), 4.65 (s, 2H), 4.12 (s, 1H), 3.90-3.80 (m, 2H), 3.44-3.38 (m, 1H), 3.16-2.95 (m, 11H), 2.47-2.40 (m, 1H), 2.30-2.23 (m, 1H), 1.71-1.61 (m, 1H), MS (ESI) m/z 556.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 6.51 (br t, J=55.2 Hz, 1H), 4.68 (dd, J=16.8 Hz and 14.8 Hz, 2H), 4.13 (s, 1H), 3.86-3.76 (m, 2H), 3.48-3.39 (m, 3H), 3.18-2.95 (m, 8H), 2.47-2.39 (m, 1H), 2.30-2.24 (m, 1H), 1.71-1.60 (m, 1H), 1.44 (t, J=7.2 Hz, 3H); MS (ESI) m/z 570.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.56-7.48 (m, 5H), 7.10 (s, 1H), 4.37 (br s, 4H), 4.12 (s, 1H), 3.38 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.14-2.95 (m, 8H), 2.43-2.35 (m, 1H), 2.27-2.22 (m, 1H), 1.70-1.69 (m, 1H); MS (ESI) m/z 568.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.89-4.83 (m, 1H), 4.17-4.10 (m, 2H), 3.47-3.37 (m, 1H), 3.18-2.95 (m, 8H), 2.76-2.72 (m, 3H), 2.48-2.22 (m, 3H), 2.04-1.95 (m, 1H), 1.91-1.70 (m, 5H), 1.70-1.50 (m, 6H), 1.33-1.20 (m, 1H); MS (ESI) m/z 588.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.58-4.48 (m, 2H), 4.13 (s, 1H), 3.42 (dd, J=16.0 Hz and 4.0 Hz, 1H), 3.23-2.95 (m, 12H), 2.46-2.39 (m, 1H), 2.30-2.27 (m, 1H), 1.88-1.78 (m, 2H), 1.70-1.60 (m, 1H), 1.47-1.36 (m, 5H), 0.98 (t, J=6.8 Hz, 3H); MS (ESI) m/z 562.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.27 (s, 1H), 4.44-4.15 (m, 5H), 3.90-3.76 (m, 2H), 3.16-2.88 (m, 10H), 3.00 (s, 3H), 2.51-2.28 (m, 4H), 1.75-1.65 (m, 1H); MS (ESI) m/z 562.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.65-4.15 (m, 7H), 3.86 (s, 1H), 3.78-3.71 (m, 1H), 3.16-3.00 (m, 10H), 2.52-2.28 (m, 4H), 1.75-1.65 (m, 1H), 1.44 (s, 3H); MS (ESI) m/z 576.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ7.14 (s, 1H), 4.37 (s, 2H), 4.13 (s, 1H), 3.40 (dd, J=16.0 Hz and 4.0 Hz, 1H), 3.17-2.97 (m, 10H), 2.45-2.35 (m, 1H), 2.30-2.23 (m, 1H), 1.77-1.64 (m, 3H), 1.48-1.43 (m, 2H), 1.00 (t, J=7.2 Hz, 3H); MS (ESI) m/z 534.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.13 (s, 1H), 4.34 (s, 2H), 4.13 (s, 1H), 3.40 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.19-2.97 (m, 10H), 2.46-2.36 (m, 1H), 2.28-2.18 (m, 3H), 2.05-1.94 (m, 1H), 1.92-1.86 (m, 4H), 1.74-1.59 (m, 1H); MS (ESI) m/z 546.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.32-7.15 (m, 5H), 7.07 (s, 1H), 4.35 (s, 2H), 4.04 (s, 1H), 3.35-3.25 (m, 3H), 3.08-2.88 (m, 10H), 2.40-2.30 (m, 1H), 2.24-2.14 (m, 1H), 1.65-1.54 (m, 1H); MS (ESI) m/z 582.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.20 (s, 1H), 4.42 (s, 2H), 4.14 (s, 1H), 3.47-3.40 (m, 1H), 3.18-2.97 (m, 9H), 2.49-2.39 (m, 1H), 2.32-2.20 (m, 2H), 1.74-1.63 (m, 1H), 1.37 (d, J=6.8 Hz, 3H), 1.09 (d, J=6.8 Hz, 3H), 1.04 (d, J=6.8 Hz, 3H); MS (ESI) m/z 547.9 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.27 and 7.24 (each s, total 1H), 4.76-4.70 (m, 1H), 4.46-4.41 and 4.27-4.15 (each m, total 1H), 4.15 (s, 1H), 3.47-3.33 (m, 2H), 3.20-2.97 (m, 8H), 2.85 (s, 3H), 2.49-2.41 (m, 1H), 2.38-2.26 (m, 1H), 2.25-2.12 (m, 1H), 1.72-1.63 (m, 1H), 1.47 and 1.42 (each d, J=6.8 Hz, total 3H), 1.20 and 1.06 (each d, J=6.4 Hz, total 3H), 1.13-1.09 (m, 3H); MS (ESI) m/z 562.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.26 and 7.23 (each s, total 1H), 4.72-4.67 (m, 1H), 4.55 and 4.31 (each d, J=14.0 Hz, total 1H), 4.15 (s, 1H), 3.46-3.30 (m, 3H), 3.20-2.97 (m, 9H), 2.48-2.40 (m, 1H), 2.38-2.26 (m, 2H), 1.72-1.62 (m, 1H), 1.44-1.31 (m, 6H), 1.19-1.13 (m, 3H), 1.09-1.05 (m, 3H); MS (ESI) m/z 576.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.29 (s, 1H), 4.60-4.42 (m, 2H), 4.13 (s, 1H), 3.60-3.51 (m, 1H), 3.46-3.38 (m, 1H), 3.25-2.86 (m, 11H), 2.48-2.38 (m, 1H), 2.32-2.24 (m, 1H), 2.06-1.94 (m, 1H), 1.90-1.80 (m, 1H), 1.70-1.53 (m, 2H), 1.53-1.43 (m, 1H), 1.13 (s, 3H), 1.03 (s, 3H); MS (ESI) m/z 574.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.69 (t, J=13.2 Hz, 1H), 4.35 (t, J=13.2 Hz, 1H), 4.13 (s, 1H), 3.47-3.36 (m, 2H), 3.20-2.95 (m, 10H), 2.46-2.39 (m, 1H), 2.29-2.26 (m, 1H), 2.20-2.10 (m, 2H), 2.01-1.92 (m, 2H), 1.82-1.58 (m, 4H), 1.50-1.37 (m, 2H), 1.34 (t, J=7.2 Hz, 3H), 1.34-1.25 (m, 1H); MS (ESI) m/z 588.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.70 (t, J=13.2 Hz, 1H), 4.39 (t, J=13.2 Hz, 1H), 4.15 (s, 1H), 3.47-3.30 (m, 2H), 3.20-2.95 (m, 9H), 2.88 (s, 3H), 2.48-2.40 (m, 1H), 2.31-2.26 (m, 1H), 1.77 (t, J=8.4 Hz, 2H), 1.73-1.61 (m, 1H), 1.01 (s, 9H); MS (ESI) m/z 576.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.60-4.55 (m, 2H), 4.14 (s, 1H), 3.47-3.42 (m, 1H), 3.20-2.96 (m, 12H), 2.52-2.43 (m, 1H), 2.33-2.26 (m, 1H), 1.77-1.65 (m, 3H), 1.42 (t, J=6.8 Hz, 3H), 1.00 (s, 9H); MS (ESI) m/z 590.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.33 (s, 1H), 4.60-4.50 (m, 2H), 4.11 (s, 1H), 3.80-3.64 (m, 4H), 3.45-3.36 (m, 1H), 3.15-2.88 (m, 9H), 2.65 (s, 6H), 2.48-2.38 (m, 1H), 2.28-2.21 (m, 1H), 2.12 (br s, 4H), 1.71-1.60 (m, 1H); MS (ESI) m/z 589.2

¹H NMR (400 MHz, CD₃OD) δ 7.36 (s, 1H), 4.60-4.52 (m, 1H), 4.14 (s, 1H), 3.82-3.60 (m, 4H), 3.46-3.38 (m, 1H), 3.20-2.96 (m, 9H), 2.68 (s, 6H), 2.52-2.40 (m, 1H), 2.30-2.24 (m, 1H), 2.20-2.09 (m, 4H), 1.48-1.40 (m, 3H); MS (ESI) m/z 603.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.58-4.48 (m, 2H), 4.13 (s, 1H), 3.42 (dd, J=16.4 Hz and 4.4 Hz, 2H), 3.20-2.96 (m, 11H), 2.48-2.38 (m, 1H), 2.31-2.23 (m, 1H), 1.90-1.77 (m, 2H), 1.72-1.60 (m, 1H), 1.39 (t, J=7.2 Hz, 3H), 1.02 (t, J=7.2 Hz, 3H); MS (ESI) m/z 548.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.17 (s, 1H), 4.58-4.50 (m, 1H), 4.43-4.36 (m, 1H), 4.13 (s, 1H), 3.42 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.20-2.93 (m, 9H), 2.85-2.77 (m, 1H), 2.46-2.36 (m, 1H), 2.30-2.26 (m, 1H), 1.70-1.60 (m, 1H), 1.50 (d, J=6.8 Hz, 3H), 1.13-1.04 (m, 1H), 0.88-0.70 (m, 2H), 0.68-0.59 (m, 1H), 0.43-0.36 (m, 1H); MS (ESI) m/z 546.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.20 (s, 1H), 4.36-4.24 (m, 2H), 4.10 (s, 1H), 3.42-3.35 (m, 1H), 3.12-2.77 (m, 12H), 2.47-2.38 (m, 1H), 2.27-2.23 (m, 1H), 1.69-1.59 (m, 1H), 1.57-1.49 (br s, 3H), 1.33-1.25 and 1.20-1.10 (each m, total 1H), 0.88-0.73 (m, 2H), 0.72-0.66 and 0.60-0.52 (each m, total 1H), 0.48-0.35 (m, 1H); MS (ESI) m/z 560.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.13 (s, 1H), 4.49 (s, 2H), 4.13 (s, 1H), 3.41 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.16-2.84 (m, 9H), 2.44-2.37 (m, 1H), 2.28-2.25 (m, 1H), 1.70-1.60 (m, 1H), 0.97-0.93 (m, 4H); MS (ESI) m/z 518.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.17 and 7.24 (each s, total 1H), 4.74, 4.67, 4.53 and 4.35 (each d, J=13.6 Hz, total 2H), 4.15 (s, 1H), 3.49-3.36 (m, 3H), 3.20-2.90 (m, 9H), 2.50-2.40 (m, 1H), 2.40-2.28 (m, 2H), 1.73-1.64 (m, 1H), 1.47-1.30 (m, 6H), 1.20-1.12 (m, 3H), 1.10-1.05 (m, 3H); MS (ESI) m/z 576.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.28 (s, 1H), 4.77-4.70 (m, 1H), 4.51-4.44 (m, 1H), 4.12 (s, 1H), 3.54-3.38 (m, 2H), 3.18-2.85 (m, 9H), 2.48-2.38 (m, 1H), 2.27-2.24 (m, 1H), 2.02-1.90 (m, 2H), 1.70-1.60 (m, 1H), 1.54 (s, 3H), 1.53 (s, 3H), 1.13-1.05 (m, 6H); MS (ESI) m/z 576.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.80-7.76 (m, 2H), 7.58-7.48 (m, 3H), 6.97-6.96 (m, 1H), 4.41-4.35 (m, 1H), 4.09-4.03 (m, 2H), 3.15-2.90 (m, 9H), 2.90-2.80 (m, 3H), 2.36-2.28 (m, 1H), 2.25-2.18 (m, 1H), 1.98 (s, 3H), 1.95 (s, 3H), 1.65-1.55 (m, 1H); MS (ESI) m/z 610.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.41 (s, 1H), 4.85-4.58 (m, 2H), 4.13 (s, 1H), 3.87-3.60 (m, 7H), 3.42 (dd, J=16.0 Hz and 4.0 Hz, 1H), 3.20-3.10 (m, 3H), 3.09-2.95 (m, 8H), 2.47-2.39 (m, 1H), 2.28-2.24 (m, 1H), 2.20-2.00 (m, 4H), 1.71-1.60 (m, 1H), 1.35-1.23 (m, 1H), 0.87-0.80 (m, 2H), 0.57-0.49 (m, 2H); MS (ESI) m/z 629.2

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.73-4.64 (m, 1H), 4.57-4.48 (m, 1H), 4.12 (s, 1H), 3.44-3.34 (m, 2H), 3.25-2.95 (m, 11H), 2.50-2.38 (m, 1H), 2.30-2.21 (m, 1H), 1.71-1.60 (m, 1H), 1.42-1.36 (m, 3H), 1.25-1.15 (m, 1H), 0.84-0.77 (m, 2H), 0.50-0.42 (m, 2H); MS (ESI) m/z 560.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.29 and 7.22 (each s, total 1H), 4.73-4.68 (m, 2H), 4.11 (s, 1H), 3.45-3.35 (m, 1H), 3.20-2.90 (m, 12H), 2.49-2.40 (m, 1H), 2.30-2.21 (m, 1H), 1.71-1.60 (m, 1H), 0.82-0.77 (m, 4H), 0.50-0.39 (m, 4H); MS (ESI) m/z 586.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.51-4.44 (m, 1H), 4.20-4.13 (m, 2H), 3.46-3.39 (m, 1H), 3.18-2.95 (m, 8H), 2.71-2.58 (m, 4H), 2.58-2.37 (m, 2H), 2.30-2.22 (m, 2H), 2.10-2.02 (m, 1H), 2.00-1.87 (m, 2H), 1.70-1.59 (m, 4H); MS (ESI) m/z 560.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.32 (s, 1H), 4.78-4.71 (m, 1H), 4.51-4.44 (m, 1H), 4.14 (s, 1H), 3.62-3.50 (m, 1H), 3.48-3.37 (m, 1H), 3.30-2.95 (m, 9H), 2.49-2.36 (m, 1H), 2.30-2.26 (m, 1H), 1.99-1.90 (m, 2H), 1.77-1.58 (m, 7H), 1.17-1.07 (m, 12H); MS (ESI) m/z 618.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.54-4.47 (m, 2H), 4.08 (s, 1H), 3.55-3.47 (m, 2H), 3.37 (dd, J=16.4 Hz and 4.8 Hz, 1H), 3.11-2.89 (m, 10H), 2.41-2.34 (m, 1H), 2.20-2.13 (m, 3H), 1.65-1.54 (m, 1H), 1.20-1.16 (m, 2H), 0.50-0.41 (m, 4H); MS (ESI) m/z 572.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.11 (s, 1H), 4.46 (s, 2H), 4.15-4.07 (m, 2H), 3.35 (dd, J=16.4 Hz and 4.8 Hz, 1H), 3.10-2.89 (m, 9H), 2.40-2.32 (m, 1H), 2.27-2.17 (m, 1H), 1.65-1.55 (m, 1H); MS (ESI) m/z 560.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.13 (s, 1H), 4.10 (s, 3H), 3.62-3.55 (m, 2H), 3.40 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.10-2.95 (m, 8H), 2.62 (s, 3H), 2.41-2.32 (m, 1H), 2.27-2.20 (m, 1H), 1.69-1.59 (m, 1H); MS (ESI) m/z 574.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.10 (s, 1H), 4.09 (s, 2H), 4.05 (s, 1H), 3.58-3.50 (m, 2H), 3.33 (dd, J=16.4 Hz and 4.8 Hz, 1H), 3.05-2.85 (m, 10H), 2.33-2.25 (m, 1H), 2.20-2.14 (m, 1H), 1.63-1.53 (m, 1H), 1.13 (t, J=6.8 Hz, 3H); MS (ESI) m/z 588.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.27 (s, 1H), 4.78-4.69 (m, 2H), 4.15 (s, 1H), 4.07-4.01 (m, 2H), 3.85-3.81 (m, 2H), 3.41 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.17-2.95 (m, 8H), 2.76-2.66 (m, 2H), 2.43-2.36 (m, 1H), 2.31-2.27 (m, 1H), 1.70-1.60 (m, 1H); MS (ESI) m/z 568.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.61-7.51 (m, 5H), 7.15 and 7.07 (each s, total 1H), 4.71-4.58 (m, 1H), 4.18-4.00 (m, 2H), 3.12-2.70 (m, 13H), 2.40-2.18 (m, 2H), 1.86 (br s, 3H), 1.66-1.52 (m, 1H); MS (ESI) m/z 596.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.61-7.50 (m, 5H), 7.03, 6.99 and 6.97 (each s, total 1H), 5.02-4.96 (m, 1H), 4.78-4.70 (m, 1H), 4.45-4.35 (m, 1H), 4.34-4.20 (m, 1H), 4.10 (s, 1H), 3.49-3.38 (m, 1H), 3.20-2.91 (m, 8H), 2.37-2.30 (m, 1H), 2.23-2.20 (m, 1H), 1.81 (d, J=6.0 Hz, 3H), 1.65-1.52 (m, 1H), 1.48-1.35 (m, 3H); MS (ESI) m/z 610.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.11 (s, 1H), 4.45 (s, 2H), 4.09 (s, 1H), 3.42-3.35 (m, 1H), 3.12-2.93 (m, 8H), 2.43-2.36 (m, 1H), 2.25-2.22 (m, 1H), 1.68-1.55 (m, 4H), 1.15-1.12 (m, 2H), 0.90-0.87 (m, 2H); MS (ESI) m/z 532.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.73-4.64 (m, 2H), 4.12 (s, 1H), 3.54-3.38 (m, 2H), 3.18-2.85 (m, 9H), 2.49-2.38 (m, 1H), 2.28-2.24 (m, 1H), 1.70-1.57 (m, 4H), 1.39 (t, J=7.2 Hz, 3H), 1.05-0.89 (m, 4H); MS (ESI) m/z 560.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.54-4.47 (m, 2H), 4.12 (s, 1H), 3.39 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.15-2.85 (m, 14H), 2.44-2.37 (m, 1H), 2.28-2.22 (m, 1H), 1.70-1.59 (m, 1H); MS (ESI) m/z 506.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25-7.20 (s, 1H), 4.55-4.40 (m, 2H), H), 4.29-4.25 (m, 1H), 4.13 (s, 1H), 3.50-3.42 (m, 2H), 3.20-2.88 (m, 10H), 2.48-2.40 (m, 1H), 2.30-2.24 (m, 1H), 2.10-2.00 (m, 1H), 1.91-1.62 (m, 6H), 1.59 and 1.52 (each d, J=6.4 Hz, total 3H) 1.70-1.59 (1H); MS (ESI) m/z 506.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.31-7.18 (m, 5H), 4.45 (s, 2H), 4.26 (m, 1H), 4.12 (s, 1H), 3.50 (dd, J=16.4 Hz and 7.6 Hz, 2H), 3.23 (dd, J=16.8 Hz and 6.4 Hz, 2H), 3.05-2.97 (m, 9H), 2.45-2.25 (m, 2H), 1.70-1.60 (m, 1H); MS (ESI) m/z 594.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.28 (s, 1H), 4.63 (d, J=11.2 Hz, 2H), 4.13 (s, 1H), 3.68-3.64 (m, 1H), 3.52-3.51 (m, 1H), 3.43-3.42 (m, 2H), 3.12-2.89 (m, 9H), 2.59 (s, 1H), 2.43-2.37 (m, 2H), 2.28-2.26 (m, 1H) 1.76-1.43 (m, 9H); MS (ESI) m/z 586.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.43 (s, 2H), 4.14 (s, 1H), 3.11-2.97 (m, 11H), 2.44-2.41 (m, 1H), 2.29-2.26 (m, 1H), 1.70-1.64 (m, 1H), 1.51-1.41 (m, 10H), 1.08 (s, 3H); MS (ESI) m/z 558.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.14 (s, 1H), 4.39 (s, 2H), 4.12 (s, 1H), 3.21-2.97 (m, 11H), 2.45-2.41 (m, 1H), 2.28-2.25 (m, 1H), 1.69-1.66 (m, 3H), 0.99 (s, 9H); MS (ESI) m/z 562.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.14 (s, 1H), 4.36 (s, 2H), 4.11 (s, 1H), 3.54-3.51 (m, 1H) 3.12-2.97 (m, 9H), 2.45-2.38 (m, 1H), 2.28-2.25 (m, 1H), 1.67-1.64 (m, 1H), 1.43 (d, J=6.4 Hz, 6H); MS (ESI) m/z 519.9 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.57-4.47 (m, 2H), 4.14 (s, 1H), 3.43-3.39 (m, 2H), 3.21-2.95 (m, 11H), 2.46-2.38 (m, 1H), 2.29-2.26 (m, 1H), 2.05-1.85 (m, 6H), 1.82-1.72 (m, 2H), 1.70-1.60 (m, 1H), 1.46 (t, J=7.2 Hz, 3H), 0.87 (t, J=6.8 Hz, 3H); MS (ESI) m/z 602.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 and 7.12 (each s, total 1H), 4.75-4.70, 4.54-4.44 and 4.34-4.27 (each m, total 2H), 4.03 (s, 1H), 3.55-3.46 (m, 1H), 3.40-3.26 (m, 1H), 3.18-2.85 (m, 10H), 2.40-2.30 (m, 1H), 2.20-2.14 (m, 1H), 1.61-1.52 (m, 1H), 1.49 and 1.25 (each t, J=6.8 Hz, total 3H), 1.40 and 1.35 (each d, J=7.2 Hz, total 3H), 1.16 (s, 3H), 0.99 (s, 3H), 0.85 (s, 3H); MS (ESI) m/z 590.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.15 (s, 1H), 4.52 (br s, 2H), 4.03 (s, 1H), 3.55 (s, 1H), 3.35-3.30 (m, 3H), 3.03-2.88 (m, 9H), 2.38-2.30 (m, 1H), 2.19-2.15 (m, 1H), 1.93-1.77 (m, 2H) 1.54-1.47 (m, 5H), 0.83-0.79 (m, 6H); MS (ESI) m/z 588.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.32 (s, 4H), 7.12 (s, 1H), 4.74-4.72 (m, 6H), 4.03 (s, 1H), 3.39-3.31 (m, 1H), 3.05-2.90 (m, 8H), 2.19-2.16 (m, 1H), 1.61-1.53 (m, 1H); MS (ESI) m/z 580.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.05 (s, 1H), 4.35 (s, 2H), 4.04 (s, 1H), 3.69-3.61 (m, 3H), 3.36-3.24 (m, 3H), 3.03-2.81 (m, 8H), 2.38-2.30 (m, 1H), 2.17-2.16 (m, 1H), 1.63-1.53 (m, 1H), 1.14-1.12 (m, 6H); MS (ESI) m/z 564.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.46-7.42 (m, 5H), 6.97 (s, 1H), 4.49-4.47 (m, 1H), 4.16-3.97 (m, 2H), 2.99-2.88 (m, 10H), 2.32-2.25 (m, 2H), 1.95-1.94 (m, 1H), 1.69-1.67 (m, 3H), 1.57-1.52 (m, 1H); MS (ESI) m/z 582.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.08 (s, 1H), 4.31 (s, 2H), 4.05 (s, 1H), 3.36-3.31 (m, 2H), 3.12-2.91 (m, 9H), 2.47-2.36 (m, 3H), 2.33-2.29 (m, 1H), 2.08-1.86 (m, 5H), 1.60-1.53 (m, 2H), 1.25-1.14 (s, 3H), 1.05-0.91 (s, 3H); MS (ESI) m/z 614.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.27-7.16 (m, 6H), 4.70-4.63 (m, 1H), 4.41-4.34 (m, 1H), 4.06 (s, 1H), 3.48-3.41 (m, 2H), 3.33-3.29 (m, 1H), 3.12-2.80 (m, 13H), 2.35-2.28 (m, 1H), 2.20-2.17 (m, 1H), 1.61-1.51 (m, 1H); MS (ESI) m/z 596.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.74 (m, 1H), 4.42 (m, 1H), 4.15 (s, 1H), 3.97-3.89 (m, 2H), 3.80-3.77 (m, 1H), 3.53 (m, 1H), 3.39 (s, 3H), 3.13-2.88 (m, 12H), 2.46-2.38 (m, 1H), 2.29-2.26 (m, 2H), 1.71-1.64 (m, 2H); MS (ESI) m/z 576.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.64-4.53 (m, 2H), 4.13 (s, 1H), 3.96-3.87 (m, 2H), 3.77-3.75 (m, 1H), 3.52-3.34 (m, 5H), 3.22-2.79 (m, 10H), 2.48-2.40 (m, 1H), 2.28-2.26 (m, 2H), 1.71-1.65 (m, 2H), 1.44-1.41 (m, 3H); MS (ESI) m/z 590.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.15 and 7.14 (each s, total 1H), 4.59-4.54 (m, 1H), 4.24-4.15 (m, 1H), 4.05 (s, 1H), 3.39-3.30 (m, 2H), 3.03-2.88 (m, 8H), 2.70 (s, 3H), 2.37-2.29 (m, 1H), 2.20-2.17 (m, 1H), 1.95-1.88 (m, 1H), 1.70-1.52 (m, 2H), 1.40-1.34 (m, 3H), 0.01-0.95 (m, 3H); MS (ESI) m/z 548.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ7.12 and 7.10 (each s, total 1H), 4.53 (m, 1H), 4.28-4.26 (m, 1H), 4.03 (s, 1H), 3.41-3.35 (m, 3H), 3.03-2.87 (m, 9H), 2.39-2.31 (m, 1H), 2.18-2.15 (m, 1H), 1.91 (m, 1H), 1.71-1.52 (m, 2H), 1.43-1.23 (m, 6H); 0.99-0.95 (m, 3H); MS (ESI) m/z 562.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.13 (s, 1H), 4.33 (s, 2H), 4.04 (s, 1H), 3.40-3.30 (m, 2H), 3.12-2.88 (m, 9H), 2.32-2.18 (m, 2H), 1.77-1.71 (m, 2H), 1.53-1.50 (m, 2H), 1.33-1.20 (m, 3H), 0.95 (s, 3H), 0.84 (d, J=9.6 Hz, 6H); MS (ESI) m/z 614.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.17 (s, 1H), 4.80-4.61 (m, 2H), 4.04 (s, 1H), 3.37-3.27 (m, 1H), 3.09-2.85 (m, 11H), 2.38-2.27 (m, 1H), 2.22-2.15 (m, 1H), 1.66-1.50 (m, 2H), 1.22-1.04 (m, 4H), 0.92-0.81 (m, 1H), 0.78-0.65 (m, 3H); MS (ESI) m+572.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.80-4.62 (m, 2H), 4.05 (s, 1H), 3.73-3.60 (m, 1H), 3.42-3.28 (m, 2H), 3.10-2.85 (m, 8H), 2.40-2.27 (m, 1H), 2.22-2.15 (m, 1H), 1.62-1.50 (m, 2H), 1.35-1.15 (m, 4H), 0.97-0.78 (m, 2H), 0.78-0.60 (m, 3H), 0.35-0.27 (m, 2H); MS (ESI) m/z 586.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.17 (s, 1H), 4.93 (br s, 2H), 4.74-4.39 (m, 2H), 3.65 (br d, J=27.2 Hz, 2H), 3.36 (dd, J=16.4 Hz and 4.4 Hz, 1H), 3.10-2.89 (m, 11H), 2.43-2.35 (m, 1H), 2.25-2.17 (m, 1H), 1.66-1.55 (m, 1H); MS (ESI) m/z 537.9 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.74-4.68 (m, 1H), 4.59-4.54 (m, 1H), 4.13 (s, 1H), 4.08-4.04 (m, 1H), 3.42 (dd, J=16.4 Hz and 4.4 Hz, 1H), 3.18-2.94 (m, 10H), 2.47-2.40 (m, 1H), 2.31-2.08 (m, 3H), 2.05-1.92 (m, 1H), 1.90-1.79 (m, 3H), 1.78-1.60 (m, 3H), 1.20-1.09 (m, 1H), 0.82-0.70 (m, 2H), 0.45-0.36 (m, 2H); MS (ESI) m/z 600.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.31-7.18 (m, 5H), 4.70-4.48 (m, 3H), 4.12 (s, 1H), 3.55-3.35 (m, 5H), 3.18-2.93 (m, 10H), 2.45-2.37 (m, 1H), 2.30-2.20 (m, 1H), 1.70-1.58 (m, 1H), 1.23-1.10 (m, 1H), 0.82-0.70 (m, 2H), 0.43-0.36 (m, 2H); MS (ESI) m/z 648.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.29 and 7.26 (each s, total 1H), 4.85-4.73 (m, 1H), 4.14 (s, 1H), 3.80-3.73 (m, 1H), 3.62-3.52 (m, 1H), 3.48-3.38 (m, 1H), 3.25-2.86 (m, 9H), 2.50-2.36 (m, 1H), 2.30-2.22 (m, 1H), 1.78-1.60 (m, 2H), 1.55-1.36 (m, 1H), 1.27-1.13 (m, 1H), 1.05-0.68 (m, 8H), 0.52-0.28 (m, 4H); MS (ESI) m/z 612.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.30 (s, 1H), 4.70-4.57 (m, 2H), 4.13 (s, 1H), 3.58-3.55 (m, 1H), 3.44-3.39 (m, 1H), 3.26-2.95 (m, 11H), 2.48-2.37 (m, 1H), 2.31-2.24 (m, 1H), 2.15-1.72 (m, 7H), 1.71-1.59 (m, 1H), 1.35-1.24 (m, 3H), 0.90-0.79 (m, 4H), 0.55-0.44 (m, 2H); MS (ESI) m/z 627.9 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.29 and 7.24 (each s, total 1H), 4.20-4.08 (m, 3H), 3.93-3.57 (m, 3H), 3.48-3.38 (m, 2H), 3.21-2.89 (m, 9H), 2.50-2.40 (m, 1H), 2.32-2.26 (m, 1H), 1.75-1.60 (m, 1H), 1.68-1.58 and 1.44-1.36 (each m, total 3H), 1.31 and 1.01 (each s, total 9H); MS (ESI) m/z 606.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.07 (s, 1H), 4.64 (s, 2H), 4.57-4.09 (m, 6H), 3.39 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.35 (s, 3H), 3.15-2.95 (m, 8H), 2.47-2.37 (m, 1H), 2.28-2.22 (m, 1H), 1.70-1.57 (m, 1H); MS (ESI) m/z 548.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.72 (t, J=12.8 Hz, 1H), 4.33 (t, J=12.4 Hz, 1H), 4.13 (s, 1H), 3.88-3.79 (m, 1H), 3.45-3.37 (m, 1H), 3.18-2.73 (m, 11H), 2.48-2.36 (m, 1H), 2.36-2.14 (m, 3H), 1.98-1.82 (m, 4H), 1.78-1.60 (m, 3H); MS (ESI) m/z 560.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.62-4.46 (m, 2H), 4.23 (s, 1H), 4.42-4.35 (m, 1H), 3.43-3.37 (m, 1H), 3.25-2.95 (m, 10H), 2.45-2.37 (m, 1H), 2.31-2.14 (m, 3H), 2.00-1.80 (m, 4H), 1.79-1.60 (m, 3H), 1.36 (t, J=6.8 Hz, 3H); MS (ESI) m/z 574.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.43-7.28 (m, 5H), 7.00 (s, 1H), 7.28 (s, 2H), 7.11 (s, 1H), 3.41 (s, 2H), 3.15-2.95 (m, 9H), 2.41-2.33 (m, 1H), 2.26-2.23 (m, 1H), 1.60-1.59 (m, 1H), 1.46 (s, 6H); MS (ESI) m/z 610.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.55-7.27 (m, 5H), 6.96 and 6.94 (each s, total 1H), 4.30-4.18 (m, 2H), 4.12 (s, 1H), 3.75-3.69 (m, 1H), 3.63-3.56 (m, 1H), 3.34 (s, 2H), 3.24-2.86 (m, 9H), 2.42-2.30 (m, 1H), 2.27-2.23 (m, 1H), 1.69-1.59 (m, 1H), 1.54 (s, 3H), 1.45 (s, 3H), 1.38-1.32 (m, 3H); MS (ESI) m/z 638.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.13 (s, 1H), 4.37 (s, 2H), 4.11 (s, 1H), 3.41 (dd, J=16.4 Hz and 4.4 Hz, 1H), 3.15-2.95 (m, 10H), 2.45-2.37 (m, 1H), 2.29-2.24 (m, 1H), 1.84-1.74 (m, 2H), 1.70-1.60 (m, 1H), 1.05 (t, J=6.8 Hz, 3H); MS (ESI) m/z 519.9 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.66 (t, J=13.6 Hz, 1H), 4.38 (t, J=12.8 Hz, 1H), 4.13 (s, 1H), 3.41 (dd, J=16.0 Hz and 4.0 Hz, 1H), 3.30-2.95 (m, 10H), 2.86 (s, 3H), 2.45-2.38 (m, 1H), 2.30-2.24 (m, 1H), 1.91-1.80 (m, 2H), 1.70-1.60 (m, 1H), 1.03 (t, J=7.2 Hz, 3H); MS (ESI) m/z 534.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.55 (t, J=14.4 Hz, 1H), 4.38 (t, J=14.4 Hz, 1H), 4.14 (s, 1H), 3.99-3.93 (m, 1H), 3.43-3.38 (m, 1H), 3.17-2.87 (m, 8H), 2.74 (s, 3H), 2.46-2.23 (m, 6H), 1.93-1.75 (m, 2H), 1.70-1.60 (m, 1H); MS (ESI) m/z 546.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.49-4.37 (m, 2H), 4.13 (s, 1H), 4.08-3.98 (m, 1H), 3.41 (dd, J=16.0 Hz and 4.0 Hz, 1H), 3.25-2.95 (m, 10H), 2.47-2.12 (m, 6H), 1.90-1.75 (m, 2H), 1.70-1.60 (m, 1H), 1.38 (t, J=6.8 Hz, 3H); MS (ESI) m/z 560.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.68-4.61 (M, 1H), 4.44-4.39 (m, 1H), 4.14 (s, 1H), 3.44-3.35 (m, 2H), 3.18-2.88 (m, 11H), 2.46-2.39 (m, 1H), 2.30-2.26 (m, 1H), 2.15-1.60 (m, 8H), 0.89 (t, J=6.8 Hz, 3H); MS (ESI) m/z 588.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.64 (t, J=13.6 Hz, 1H), 4.38 (t, J=12.8 Hz, 1H), 4.14 (s, 1H), 3.45-3.36 (m, 2H), 3.18-2.95 (m, 9H), 2.86 (s, 3H), 2.46-2.38 (m, 1H), 2.29-2.26 (m, 1H), 1.70-1.60 (m, 1H), 1.45 (t, J=6.8 Hz, 3H); MS (ESI) m/z 520.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.55-4.48 (m, 2H), 4.13 (s, 1H), 3.44-3.39 (m, 1H), 3.18-2.86 (m, 12H), 2.46-2.39 (m, 1H), 2.28-2.25 (m, 1H), 1.70-1.60 (m, 1H), 1.44-1.37 (m, 6H); MS (ESI) m/z 534.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.81-4.74 (m, 1H), 4.44-4.39 (m, 1H), 4.13 (s, 1H), 3.84 (br s, 2H), 3.76-3.68 (m, 1H), 3.49 (br s, 2H), 3.41 (dd, J=16.4 Hz and 4.4 Hz, 1H), 3.18-2.85 (m, 11H), 2.46-2.39 (m, 1H), 2.29-2.26 (m, 1H), 1.70-1.60 (m, 1H), 1.22-1.18 (m, 6H); MS (ESI) m/z 578.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.72-4.66 (m, 1H), 4.57-4.52 (m, 1H), 4.11 (s, 1H), 3.83-3.81 (m, 2H), 3.72-3.66 (m, 1H), 3.53-3.47 (m, 1H), 3.45-3.34 (m, 4H), 3.17-2.95 (m, 9H), 2.47-2.39 (m, 1H), 2.28-2.24 (m, 1H), 1.70-1.60 (m, 1H), 1.50 (t, J=6.8 Hz, 3H), 1.21-1.13 (m, 6); MS (ESI) m/z 592.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.80-4.70 (m, 2H), 4.13 (s, 1H), 3.41 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.18-2.96 (m, 12H), 2.46-2.39 (m, 1H), 2.29-2.25 (m, 1H), 1.70-1.60 (m, 1H), 1.10-0.82 (m, 4H); MS (ESI) m/z 532.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.76-4.60 (m, 2H), 4.13 (s, 1H), 3.49-3.39 (m, 3H), 3.18-2.95 (m, 9H), 2.47-2.39 (m, 1H), 2.28-2.25 (m, 1H), 1.70-1.61 (m, 1H), 1.49 (t, J=7.6 Hz, 3H), 1.10-0.80 (m, 3H), 0.75-0.60 (m, 1H); MS (ESI) m/z 546.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.82-4.76 (m, 1H), 4.33-4.27 (m, 1H), 4.13 (s, 1H), 3.75-3.65 (m, 1H), 3.47-3.39 (m, 1H), 3.18-2.75 (m, 11H), 2.51-2.39 (m, 1H), 2.29-2.25 (m, 1H), 2.20-1.76 (m, 4H), 1.70-1.40 (m, 4H), 1.30 (s, 3H), 0.99-0.96 (m, 6H); MS (ESI) m/z 628.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25 and 7.24 (each s, total 1H), 4.82-4.73 (m, 1H), 4.53-4.44 (m, 1H), 4.13 (s, 1H), 3.88-3.71 (m, 1H), 3.48-3.32 (m, 3H), 3.18-2.95 (m, 8H), 2.54-2.36 (m, 2H), 2.29-2.25 (m, 1H), 1.98-1.85 (m, 2H), 1.84-1.75 (m, 1H), 1.70-1.60 (m, 1H), 1.59-1.40 (m, 3H), 1.38-1.27 (m, 6H), 1.00-0.93 (m, 6H); MS (ESI) m/z 642.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.29 (s, 1H), 4.97-4.94 (m, 1H), 4.68-4.65 (m, 1H), 4.17-4.06 (m, 3H), 3.45-3.40 (m, 2H), 3.18-2.95 (m, 11H), 2.45-2.38 (m, 1H), 2.30-2.26 (m, 1H), 1.70-1.61 (m, 1H), 1.05 (s, 9H); MS (ESI) m/z 592.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.75-4.67 (m, 2H), 4.12 (s, 1H), 3.88-3.76 (m, 2H), 3.75-3.56 (m, 2H), 3.55-3.37 (m, 2H), 3.28-2.95 (m, 10H), 2.46-2.39 (m, 1H), 2.29-2.25 (m, 1H), 1.71-1.61 (m, 1H), 1.30-1.10 (m, 7H), 0.85-0.74 (m, 2H), 0.51-0.42 (m, 2H); MS (ESI) m/z 618.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.70-4.59 (m, 2H), 4.12 (s, 1H), 3.42 (dd, J=16.4 Hz and 4.4 Hz, 1H), 3.25-2.95 (m, 12H), 2.47-2.40 (m, 1H), 2.29-2.25 (m, 1H), 1.92-1.60 (m, 7H), 1.40-1.12 (m, 4H), 1.10-0.90 (m, 2H), 0.89-0.78 (m, 2H), 0.52-0.45 (m, 2H); MS (ESI) m/z 628.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.75-4.60 (m, 2H), 4.13 (s, 1H), 3.97-3.70 (m, 2H), 3.69-3.61 (m, 1H), 3.55-3.38 (m, 3H), 3.28-2.95 (m, 11H), 2.86-2.76 (m, 1H), 2.48-2.40 (m, 1H), 2.31-2.19 (m, 2H), 1.76-1.57 (m, 2H), 1.30-1.20 (m, 1H), 0.84-0.82 (m, 2H), 0.50-0.49 (m, 2H); MS (ESI) m/z 616.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.72-4.66 (m, 1H), 4.58-4.55 (m, 1H), 4.13 (s, 1H), 3.42 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.28-2.95 (m, 12H), 2.48-2.40 (m, 1H), 2.30-2.25 (m, 1H), 1.78-1.60 (m, 4H), 1.25-1.16 (m, 1H), 0.98-0.95 (m, 6H) 0.83-0.79 (m, 2H) 0.48-0.46 (m, 2H): MS (ESI) m/z 602.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.75-4.66 (m, 1H), 4.60-4.52 (m, 1H), 4.12 (s, 1H), 3.47-3.38 (m, 1H), 3.25-2.95 (m, 12H), 2.48-2.40 (m, 1H), 2.30-2.25 (m, 1H), 1.78-1.61 (m, 3H), 1.25-1.16 (m, 1H), 0.94 (s, 9H), 0.83-0.80 (m, 2H), 0.49-0.47 (m, 2H); MS (ESI) m/z 616.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.74-4.61 (m, 2H), 4.13 (s, 1H), 3.44-3.40 (m, 2H), 3.28-2.95 (m, 11H), 2.47-2.35 (m, 2H), 2.29-2.26 (m, 1H), 2.00-1.90 (m, 1H), 1.75-1.59 (m, 5H), 1.38-1.16 (m, 3H), 0.85-0.78 (m, 2H), 0.51-0.46 (m, 2H); MS (ESI) m/z 614.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.16 (s, 1H), 4.73 (br s, 1H), 4.48 (dd, J=27.6 Hz and 16.0 Hz, 2H), 4.10 (br s, 1H), 3.69-3.46 (m, 2H), 3.40-3.29 (m, 1H), 3.10-2.88 (m, 7H), 2.38-2.20 (m, 2H), 1.98-1.72 (m, 5H), 1.71-1.50 (m, 2H), 1.40-1.18 (m, 6H); MS (ESI) m/z 574.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.13 (s, 1H), 4.38 (s, 2H), 4.11 (s, 1H), 3.41 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.22-2.87 (m, 10H), 2.45-2.37 (m, 1H), 2.28-2.25 (m, 1H), 1.73-1.64 (m, 4H), 1.01-0.97 (m, 6H); MS (ESI) m/z 548.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.62 (dd, J=18.4 Hz and 13.6 Hz, 2H), 4.11 (s, 1H), 3.86-3.81 (m, 2H), 3.42 (dd, J=16.0 Hz and 4.8 Hz, 1H), 3.18-2.95 (m, 8H), 2.49-2.41 (m, 1H), 2.37-2.24 (m, 3H), 1.83-1.76 (m, 2H), 1.72-1.62 (m, 1H), 1.37-1.33 (m, 6H); MS (ESI) m/z 560.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.20 (s, 1H), 4.61 (t, J=12.8 Hz, 1H), 4.33 (t, J=12.8 Hz, 1H), 4.13 (s, 1H), 3.41 (dd, J=16.4 Hz and 4.4 Hz, 1H), 3.36-3.30 (m, 1H), 3.18-2.80 (m, 12H), 2.46-2.38 (m, 1H), 2.29-1.18 (m, 3H), 2.10-2.00 (m, 1), 2.00-1.85 (m, 4H), 1.70-1.60 (m, 1H); MS (ESI) m/z 560.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.52-4.42 (m, 2H), 4.12 (s, 1H), 3.45-3.38 (m, 1H), 3.28-2.80 (m, 12H), 2.49-2.40 (m, 1H), 2.30-2.13 (m, 3H), 2.08-1.97 (m, 1H), 1.96-1.80 (m, 4H), 1.70-1.60 (m, 1H), 1.38 (t, J=7.2 Hz, 3H); MS (ESI) m/z 574.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.23 and 7.20 (each s, total 1H), 4.68-4.42 (m, 2H), 4.13 (s, 1H), 3.85-3.75 (m, 1H), 3.47-3.38 (m, 1H), 3.18-2.85 (m, 10H), 2.46-2.01 (m, 3H), 1.73-1.49 (m, 10H); MS (ESI) m/z 600.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.70 (d, J=13.2 Hz, 1H), 4.42 (d, J=13.2 Hz, 1H), 4.06 (s, 1H), 3.40 (dd, J=16.4 Hz and 4.8 Hz, 1H), 3.12-2.80 (m, 12H), 2.44-2.36 (m, 1H), 2.23-2.20 (m, 1H), 1.67-1.57 (m, 1H), 0.97 (s, 9H); MS (ESI) m/z 576.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.16 (s, 1H), 4.40 (s, 2H), 4.12-4.05 (m, 4H), 3.90-3.84 (m, 1H), 3.78-3.71 (m, 1H), 3.44-3.37 (m, 1H), 3.15-2.95 (m, 8H), 2.50-2.40 (m, 2H), 2.28-2.23 (m, 1H), 2.20-2.09 (m, 1H), 1.71-1.60 (m, 1H); MS (ESI) m/z 548.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.16 (s, 1H), 4.32 (t, J=15.6 Hz, 1H), 4.17 (t, J=15.6 Hz, 1H), 4.11 (s, 1H), 3.90-3.80 (m, 1H), 3.40 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.29-3.20 (m, 1H), 3.10-2.95 (m, 8H), 2.83-2.74 (m, 1H), 2.41-2.20 (m, 3H), 2.12-1.90 (m, 3H), 1.70-1.60 (m, 1H); MS (ESI) m/z 599.9 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.66 (t, J=12.8 Hz, 1H), 4.37 (t, J=12.8 Hz, 1H), 4.13 (s, 1H), 3.43-3.39 (m, 1H), 3.20-2.95 (m, 9H), 2.86 (s, 3H), 2.46-2.39 (m, 1H), 2.28-2.25 (m, 1H), 1.80-1.60 (m, 8H), 1.44-1.12 (m, 4H), 1.08-0.96 (m, 2H); MS (ESI) m/z 602.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.53 (s, 2H), 4.11 (s, 1H), 3.71-3.62 (m, 2H), 3.40 (dd, J=16.4 Hz and 4.8 Hz, 1H), 3.16-2.95 (m, 10H), 2.70-2.60 (m, 1H), 2.45-2.38 (m, 1H), 2.27-2.23 (m, 1H), 2.17-2.14 (m, 2H), 1.96-1.83 (m, 2H), 1.70-1.59 (m, 1H); MS (ESI) m/z 614.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.82-4.77 (m, 1H), 4.39-4.36 (m, 1H), 4.10 (s, 1H), 3.92-3.85 (m, 1H), 3.76-3.72 (m, 1H), 3.68-3.60 (m, 1H), 3.51-3.32 (m, 5H), 3.17-2.95 (m, 9H), 2.45-2.37 (m, 1), 2.24-2.31 (m, 2H), 2.20-2.10 (m, 1H), 2.05-1.87 (m, 2H), 1.70-1.59 (m, 1H); MS (ESI) m/z 576.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.10 (s, 1H), 4.36 (s, 2H), 4.11 (s, 1H), 3.40 (dd, J=16.0 Hz and 4.4 Hz, 1H), 3.16-2.95 (m, 8H), 2.81 (s, 3H), 2.45-2.37 (m, 1H), 2.27-2.24 (m, 1H), 1.70-1.60 (m, 1H); MS (ESI m/z 492.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.50 (d, J=13.7 Hz, 1H), 4.43 (d, J=13.7 Hz, 1H), 4.12 (s, 1H), 3.48-3.38 (m, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 3.16-2.94 (comp, 3H), 2.49-2.39 (m, 1H), 2.30-2.23 (m, 1H), 1.72-1.61 (m, 1H), 1.40 (d, J=7.3 Hz, 3H), 1.05 (s, 9H); MS (ESI) m/z 562.22 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.16 (s, 1H), 4.52 (d, J=13.4 Hz, 1H), 4.39 (d, J=13.4 Hz, 1H), 4.11 (s, 1H), 3.49-3.36 (m, 1H), 3.05 (s, 3H), 2.96 (s, 3H), 3.14-2.94 (comp, 3H), 2.48-2.38 (m, 1H), 2.29-2.22 (m, 1H), 1.73-1.62 (m, 1H), 1.50 (d, J=6.7 Hz, 3H), 1.12-1.03 (m, 1H), 0.87-0.73 (m, 2H), 0.67-0.59 (m, 1H), 0.44-0.36 (m, 1H); MS (ESI) m/z 546.16 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.49 (s, 2H), 4.11 (s, 1H), 3.60-3.37 (comp, 4H), 3.05 (s, 3H), 2.97 (s, 3H), 3.23-2.94 (comp, 3H), 2.48-2.39 (m, 1H), 2.28-2.21 (m, 1H), 1.97-1.88 (m, 2H), 1.80-1.61 (m, 2H), 1.53-1.38 (m, 2H), 1.01 (d, J=6.1 Hz, 3H); MS (ESI) m/z 560.19 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.31 (s, 1H), 4.71 (d, J=13.4 Hz, 1H), 4.47 (d, J=13.4 Hz, 1H), 4.11 (s, 1H), 3.48-3.38 (m, 1H), 3.04 (s, 3H), 2.96 (s, 3H), 2.90 (s, 3H), 3.16-2.86 (comp, 3H), 2.50-2.40 (m, 1H), 2.29-2.22 (m, 1H), 1.72-1.60 (m, 1H), 1.43 (d, J=6.7 Hz, 3H), 1.02 (s, 9H); MS (ESI) m/z 576.20 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.57 (s, 2H), 4.11 (s, 1H), 3.85-3.76 (m, 2H), 3.57-3.38 (m, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 3.15-2.81 (comp, 4H), 2.49-2.39 (m, 1H), 2.29-2.21 (m, 1H), 1.95-1.47 (comp, 9H); MS (ESI) m/z 572.14 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.21-7.18 (m, 1H), 4.54-4.24 (m, 2H), 4.10 (s, 1H), 3.49-3.35 (comp, 3H), 3.04 (s, 3H), 2.96 (s, 3H), 3.14-2.93 (comp, 3H), 2.49-2.38 (m, 1H), 2.28-2.21 (m, 1H), 2.07-2.00 (m, 1H), 1.91-1.49 (comp, 9H); MS (ESI) m/z 560.15 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.50 (d, J=13.7 Hz, 1H), 4.46 (d, J=13.7 Hz, 1H), 4.12 (s, 1H), 3.57-3.36 (comp, 3H), 3.05 (s, 3H), 2.96 (s, 3H), 3.20-2.94 (comp, 4H), 2.47-2.37 (m, 1H), 2.30-2.21 (m, 1H), 1.99-1.47 (comp, 7H), MS (ESI) m/z 546.10 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.20 (s, 1H), 4.58 (s, 2H), 4.11 (s, 1H), 3.69-3.56 (m, 2H), 3.48-3.36 (m, 2H), 3.04 (s, 3H), 2.96 (s, 3H), 3.16-2.93 (comp, 3H), 2.46-2.37 (m, 1H), 2.29-1.98 (comp, 5H), 1.71-1.59 (m, 1H); MS (ESI) m/z 532.09 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.53 (s, 2H), 4.11 (s, 1H), 3.54-3.33 (comp, 5H), 3.04 (s, 3H), 2.96 (s, 3H), 3.14-2.93 (comp, 2H), 2.47-2.38 (m, 1H), 2.28-2.21 (m, 1H), 2.06-1.60 (comp, 9H); MS (ESI) m/z 560.15 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25-7.23 (m, 1H), 4.53-4.47 (m, 2H), 4.12 (s, 1H), 3.63-3.34 (comp, 8H), 3.04 (s, 3H), 2.96 (s, 3H), 3.14-2.94 (comp, 3H), 2.47-2.37 (m, 1H), 2.31-1.60 (comp, 6H); MS (ESI) m/z 576.15 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.34-7.24 (comp, 4H), 7.21-7.17 (m, 1H), 4.69 (s, 2H), 4.54 (s, 2H), 4.11 (s, 1H), 3.90-3.53 (m, 2H), 3.47-3.39 (m, 2H), 3.04 (s, 3H), 2.96 (s, 3H), 3.28-2.94 (comp, 3H), 2.50-2.40 (m, 1H), 2.29-2.22 (m, 1H), 1.72-1.61 (m, 1H); MS (ESI) m/z 594.15 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.32-7.26 (m, 2H), 6.99-6.94 (m, 2H), 4.93-4.64 (comp, 6H), 4.13 (s, 1H), 3.81 (s, 3H), 3.46-3.33 (m, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 3.18-2.95 (comp, 2H), 2.50-2.39 (m, 1H), 2.30-2.23 (m, 1H), 1.73-1.61 (m, 1H); MS (ESI) m/z 610.15 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.44 (s, 2H), 4.10 (s, 1H), 3.71 (s, 4H), 3.47 (s, 4H), 3.44-3.33 (m, 1H), 3.04 (s, 3H), 2.95 (s, 3H), 3.15-2.93 (comp, 2H), 2.45-2.36 (m, 1H), 2.27-2.20 (m, 1H), 1.71-1.59 (m, 1H); MS (ESI) m/z 596.08 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.55 (d, J=13.7 Hz, 1H), 4.51 (d, J=13.7 Hz, 1H), 4.12 (s, 1H), 3.56-3.31 (comp, 5H), 3.05 (s, 3H), 2.96 (s, 3H), 3.16-2.94 (comp, 2H), 2.48-2.36 (m, 1H), 2.30-2.22 (m, 1H), 2.14-1.59 (comp, 11H); MS (ESI) m: 574.18 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.81 (d, J=13.4 Hz, 1H), 4.31 (d, J=13.4 Hz, 1H), 4.12 (s, 1H), 3.75-3.33 (comp, 4H), 3.06 (s, 3H), 2.97 (s, 3H), 3.18-2.94 (comp, 2H), 2.50-1.61 (comp, 7H), 1.53 (d, J=6.1 Hz, 3H); MS (ESI) m/z 546.20 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.77 (d, J=13.4 Hz, 1H), 4.33 (d, J=13.4 Hz, 1H), 4.13 (s, 1H), 3.75-3.32 (comp, 4H), 3.05 (s, 3H), 2.96 (s, 3H), 3.17-2.94 (comp, 2H), 2.46-1.97 (comp, 5H), 1.84-1.60 (comp, 2H), 1.53 (d, J=6.1 Hz, 3H); MS (ESI) m/z 546.16 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.07 (s, 1H), 4.59 (s, 2H), 4.35-4.20 (m, 4H), 4.11 (s, 1H), 3.43-3.37 (m, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 3.15-2.95 (comp, 2H), 2.67-2.20 (comp, 4H), 1.72-1.61 (m, 1H); MS (ESI) m/z 518.11 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 5.58-5.39 (m, 1H), 4.68 (s, 2H), 4.12 (s, 1H), 4.03-3.38 (comp, 5H), 3.05 (s, 3H), 2.97 (s, 3H), 3.17-2.95 (comp, 2H), 2.83-2.20 (comp, 4H), 1.73-1.61 (m, 1H); MS (ESI) m/z 550.16 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.30-7.21 (m, 1H), 4.66-4.51 (m, 2H), 4.11 (s, 1H), 3.84-3.31 (comp, 5H), 3.05 (s, 3H), 2.97 (s, 3H), 3.16-2.95 (comp, 2H), 2.87-2.61 (m, 2H), 2.50-2.40 (m, 1H), 2.33-1.83 (comp, 5H), 1.73-1.61 (m, 1H); MS (ESI) m/z 574.18 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.37 (s, 1H), 4.62 (d, J=14.0 Hz, 1H), 4.57 (d, J=14.0 Hz, 1H), 4.12 (s, 1H), 3.75-3.66 (m, 2H), 3.48-3.32 (comp, 3H), 3.04 (s, 3H), 2.96 (s, 3H), 3.17-2.94 (comp, 2H), 2.48-2.37 (m, 1H), 2.29-2.22 (m, 1H), 2.22-2.13 (br, 2H), 1.98-1.60 (comp, 9H); MS (ESI) m/z 586.24 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.26-7.22 (m, 1H), 4.60-4.48 (m, 2H), 4.12 (s, 1H), 4.12-4.01 (m, 1H), 3.73-3.33 (comp, 4H), 3.05 (s, 3H), 2.97 (s, 3H), 3.29-2.95 (comp, 3H), 2.49-2.39 (m, 1H), 2.30-1.61 (comp, 8H); MS (ESI) m/z 576.22 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.31 (s, 1H), 7.23 (s, 4H), 4.62 (d, J=14.3 Hz, 1H), 4.58 (d, J=14.3 Hz, 1H), 4.12 (s, 1H), 3.85-3.75 (m, 2H), 3.06 (s, 3H), 2.97 (s, 3H), 3.63-2.94 (comp, 9H), 2.50-2.41 (m, 1H), 2.30-2.23 (m, 1H), 1.73-1.62 (m, 1H); MS (ESI) m/z 608.21 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.23-7.15 (m, 1H), 4.74-4.61 (m, 1H), 4.55-4.33 (m, 1H), 4.11 (s, 1H), 3.98-3.37 (comp, 3H), 3.04 (s, 3H), 2.96 (s, 3H), 3.16-2.94 (comp, 3H), 2.50-2.37 (m, 1H), 2.30-2.21 (m, 1H), 2.10-1.50 (comp, 12H); MS (ESI) m/z 574.32 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.64 (d, J=13.2 Hz, 1H), 4.58 (d, J=13.2 Hz, 1H), 4.11 (s, 1H), 3.88-3.78 (m, 2H), 3.49-3.38 (m, 1H), 3.04 (s, 3H), 2.96 (s, 3H), 3.16-2.93 (comp, 2H), 2.49-2.21 (comp, 4H), 1.84-1.61 (m, 3H), 1.38-1.32 (m, 6H); MS (ESI) m/z 560.27 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.50 (d, J=13.7 Hz, 1H), 4.46 (d, J=13.7 Hz, 1H), 4.14 (s, 1H), 3.48-3.36 (m, 1H), 3.06 (s, 3H), 2.97 (s, 3H), 3.16-2.95 (comp, 3H), 2.48-2.37 (m, 1H), 2.32-2.23 (m, 1H), 1.72-1.60 (m, 1H), 1.40 (d, J=6.7 Hz, 3H), 1.05 (s, 9H); MS (ESI) m/z 562.23 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.42 (s, 2H), 4.14 (s, 1H), 3.31 (br s, 1H), 3.39-3.45 (m, 1H), 2.97-3.14 (m, 8H), 2.26-2.44 (m, 3H), 1.61-2.06 (m, 14H); MS (ESI) m/z 612.3 (M+H), calcd for C₃₂H₃₉ClN₃O₇ 612.24.

¹H NMR (400 MHz, CD₃OD) δ 7.27 (s, 1H), 4.71-4.87 (m, 2H), 4.14 (s, 1H), 3.37-3.46 (m, 2H), 2.94-3.28 (m, 10H), 2.42 (dd, J=14.0, 14.0 Hz, 1H), 2.24-2.32 (m, 1H), 1.60-1.70 (m, 1H), 1.27-1.36 (m, 1H), 0.51-1.14 (m, 8H); MS (ESI) m/z 572.3 (M+H), calcd for C₂₉H₃₅ClN₃O₇ 572.21.

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.60-5.00 (m, 4H), 4.14 (s, 1H), 3.64-3.82 (m, 2H), 3.20-3.46 (m, 3H), 2.94-3.20 (m, 8H), 2.41 (dd, J=14.0, 14.0 Hz, 1H), 2.25-2.29 (m, 1H), 1.60-1.70 (m, 1H), 1.24-1.28 (m, 1H), 0.78-0.86 (m, 2H), 0.46-0.52 (m, 2H); MS (ESI) m/z 578.3 (M+H), calcd for C₂₈H₃₄ClFN₃O₇ 578.20.

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.63-5.00 (m, 4H), 4.13 (s, 1H), 3.61-3.72 (m, 2H), 3.20-3.43 (m, 3H), 2.94-3.20 (m, 8H), 2.42 (dd, J=14.0, 14.0 Hz, 1H), 2.25-2.29 (m, 1H), 1.85-1.88 (m, 2H), 1.60-1.70 (m, 1H), 1.01 (t, J=7.3 Hz, 3H); MS (ESI) m/z 566.3 (M+H), calcd for C₂₇H₃₄FN₃O₇ 566.20.

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.72 (dd, J=13.7, 13.7 Hz, 1H), 4.34 (dd, J=10.2, 10.2 Hz, 1H), 4.14 (s, 1H), 3.38-3.44 (m, 1H), 2.87-3.21 (m, 13H), 2.42 (dd, J=14.0, 14.0 Hz, 1H), 2.23-2.31 (m, 2H), 1.60-1.70 (m, 1H), 1.09 (d, J=6.5 Hz, 3H), 1.05 (d, J=6.5 Hz, 3H); MS (ESI) m/z 548.3 (M+H), calcd for C₂₇H₃₅ClFN₃O₇ 548.21.

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.63 (dd, J=13.7, 13.7 Hz, 1H), 4.47 (dd, J=10.2, 10.2 Hz, 1H), 4.14 (s, 1H), 3.29-3.44 (m, 3H), 2.94-3.21 (m, 10H), 2.43 (dd, J=14.0, 14.0 Hz, 1H), 2.18-2.29 (m, 2H), 1.60-1.70 (m, 1H), 1.40 (t, J=7.3 Hz, 3H), 1.09 (d, J=6.5 Hz, 3H), 1.03 (d, J=6.5 Hz, 3H); MS (ESI) m/z 562.3 (M+H), calcd for C₂₈H₃₇ClN₃O₇ 562.22.

¹H NMR (400 MHz, CD₃OD) δ 7.20 (s, 1H), 4.75-4.80 (m, 1H), 4.33-4.37 (m, 1H), 4.11 (s, 1H), 2.88-3.49 (m, 16H), 2.43 (dd, J=15.0, 15.0 Hz, 1H), 2.23-2.26 (m, 1H), 1.60-1.70 (m, 1H), 1.19-1.28 (m, 1H), 0.80-0.82 (m, 2H), 0.46-0.50 (m, 2H); MS (ESI) m/z 546.2 (M+H), calcd for C₂₇H₃₃ClN₃O₇ 546.19.

¹H NMR (400 MHz, CD₃OD) δ 7.20 (s, 1H), 4.72 (dd, J=12.8, 12.8 Hz, 1H), 4.32 (dd, J=4.4, 12.8 Hz, 1H), 4.12 (s, 1H), 3.40 (dd, J=4.6, 16.0 Hz, 1H), 2.87-3.14 (m, 13H), 2.43 (dd, J=15.0, 15.0 Hz, 1H), 2.24-2.28 (m, 1H), 1.60-1.98 (m, 7H), 1.21-1.42 (m, 3H), 1.01-1.12 (m, 2H); MS (ESI) m/z 588.3 (M+H), calcd for C₃₀H₃₉ClN₃O₇ 588.24.

¹H NMR (400 MHz, CD₃OD) δ 7.20 (s, 1H), 4.83-4.95 (m, 1H), 4.14-4.19 (m, 1H), 4.10 (s, 1H), 2.74-3.50 (m, 12H), 2.42 (dd, J=15.0, 15.0 Hz, 1H), 2.22-2.28 (m, 1H), 1.90-2.20 (m, 2H), 1.60-1.70 (m, 1H), 1.53 (s, 3H), 1.50 (s, 3H), 1.08 (t, J=7.3 Hz, 3H); MS (ESI) m/z 562.3 (M+H), calcd for C₂₈H₃₇ClN₃O₇ 562.22.

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.91-4.99 (m, 1H), 4.18-4.22 (m, 1H), 4.12 (s, 1H), 3.41 (dd, J=4.6, 16.0 Hz, 1H), 2.96-3.10 (m, 11H), 2.45 (dd, J=15.0, 15.0 Hz, 1H), 2.24-2.30 (m, 1H), 1.61-1.71 (m, 1H), 1.42 (s, 6H), 1.28-1.36 (m, 1H), 0.76-0.81 (m, 2H), 0.64-0.72 (m, 2H); MS (ESI) m/z 574.2 (M+H), calcd for C₂₉H₃₇ClN₃O₇ 574.22.

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.46-4.63 (m, 2H), 4.14 (s, 1H), 3.95-4.02 (m, 1H), 3.40 (dd, J=4.6, 16.0 Hz, 1H), 2.96-3.21 (m, 10H), 2.41 (dd, J=15.0, 15.0 Hz, 1H), 2.26-2.29 (m, 1H), 1.60-1.70 (m, 1H), 1.50 (d, J=6.4 Hz, 3H), 1.41 (d, J=6.4 Hz, 3H), 1.06-1.17 (m, 1H), 0.72-0.78 (m, 2H), 0.42-0.44 (m, 2H); MS (ESI) m/z 574.2 (M+H), calcd for C₂₉H₃₇ClN₃O₇ 574.22.

¹H NMR (400 MHz, CD₃OD) δ 7.30 (s, 1H), 4.50-4.60 (m, 2H), 4.14 (s, 1H), 3.78-3.84 (m, 1H), 3.42 (dd, J=4.6, 16.0 Hz, 1H), 2.94-3.21 (m, 10H), 2.42 (dd, J=15.0, 15.0 Hz, 1H), 2.26-2.30 (m, 1H), 1.96-2.02 (m, 1H), 1.60-1.70 (m, 1H), 1.50 (d, J=6.4 Hz, 3H), 1.44 (d, J=6.4 Hz, 3H), 1.03 (d, J=6.4 Hz, 3H), 0.94 (d, J=6.4 Hz, 3H); MS (ESI) m/z 576.3 (M+H), calcd for C₂₉H₃₉ClN₃O₇ 576.24.

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.63 (dd, J=14.2, 14.2 Hz, 1H), 4.40 (dd, J=14.2, 14.2 Hz, 1H), 4.14 (s, 1H), 3.78-3.84 (m, 1H), 3.42 (dd, J=4.6, 16.0 Hz, 1H), 2.96-3.28 (m, 10H), 2.42 (dd, J=15.0, 15.0 Hz, 1H), 2.26-2.29 (m, 1H), 1.54-1.70 (m, 4H), 1.51 (d, J=6.4 Hz, 3H), 1.43 (d, J=6.4 Hz, 3H), 0.89-0.94 (m, 6H); MS (ESI) m/z 590.3 (M+H), calcd for C₃₀H₄₁ClN₃O₇ 590.26.

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.74 (dd, J=14.2, 14.2 Hz, 1H), 4.34 (dd, J=8.7, 14.2 Hz, 1H), 4.13 (s, 1H), 3.41 (dd, J=4.6, 16.0 Hz, 1H), 2.88-3.34 (m, 13H), 2.39-2.46 (m, 2H), 2.26-2.28 (m, 1H), 1.96-2.01 (m, 2H), 1.61-1.74 (m, 5H), 1.23-1.35 (m, 2H); MS (ESI) m/z 574.2 (M+H), calcd for C₂₉H₃₇ClN₃O₇ 574.22.

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.62 (dd, J=14.2, 14.2 Hz, 1H), 4.48 (dd, J=8.7, 14.2 Hz, 1H), 4.14 (s, 1H), 3.41 (dd, J=4.6, 16.0 Hz, 1H), 2.96-3.34 (m, 12H), 2.26-2.41 (m, 3H), 1.96-2.01 (m, 2H), 1.61-1.74 (m, 5H), 1.40 (t, J=7.3 Hz, 3H), 1.22-1.32 (m, 2H); MS (ESI) m/z 588.3 (M+H), calcd for C₃₀H₃₇ClN₃O₇ 588.24.

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.75 (dd, J=9.6, 12.8 Hz, 1H), 4.28 (dd, J=9.6, 12.8 Hz, 1H), 4.12 (s, 1H), 3.92 (d, J=12.1 Hz, 1H), 3.71 (d, J=12.1 Hz, 1H), 3.38-3.47 (m, 1H), 2.97-3.16 (m, 8H), 2.72 (s, 3H), 2.38-2.47 (m, 1H), 2.25-2.28 (m, 1H), 1.60-1.70 (m, 1H), 1.51 (s, 3H), 1.46 (s, 3H); MS (ESI) m/z 564.3 (M+H), calcd for C₂₇H₃₅ClN₃O₈ 564.20.

¹H NMR (400 MHz, CD₃OD) δ 7.21-7.16 (m, 1H), 4.78-4.49 (m, 2H), 4.12 (s, 1H), 3.71-3.32 (comp, 3H), 3.16-2.95 (comp, 2H), 3.04 (s, 3H), 2.96 (s, 3H), 2.46-2.36 (m, 1H), 2.30-2.22 (m, 1H), 2.14-1.58 (comp, 7H), 1.45-1.26 (comp, 6H); MS (ESI) m/z 574.18 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.44 (s, 2H), 4.23 (s, 2H), 4.10 (s, 1H), 3.48-3.36 (comp, 1H), 3.04 (s, 3H), 2.96 (s, 3H), 3.14-2.94 (comp, 2H), 2.49-2.02 (comp, 6H), 1.98-1.81 (m, 4H), 1.72-1.61 (m, 1H); MS (ESI) m/z 558.21 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.28 (s, 1H), 4.68-4.48 (m, 2H), 4.13 (s, 1H), 4.12-4.02 (m, 1H), 3.90-3.80 (m, 1H), 3.49-3.38 (m, 1H), 3.05 (s, 3H), 2.97 (s, 3H), 3.17-2.95 (comp, 2H), 2.52-2.23 (comp, 4H), 1.98-1.60 (comp, 3H), 1.48-1.34 (m, 6H); MS ESI m/z 560.28 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.52 (s, 2H), 4.11 (s, 1H), 4.10-4.00 (m, 1H), 3.90-3.80 (m, 1H), 3.46-3.37 (m, 1H), 3.05 (s, 3H), 2.96 (s, 3H), 3.16-2.90 (comp, 2H), 2.52-2.12 (m, 4H), 1.98-1.58 (m, 3H), 1.48-1.33 (comp, 6H); MS (ESI) m/z 560.27 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.68 (d, J=14.4 Hz, 1H), 4.51 (d, J=14.4 Hz, 1H), 4.11 (s, 1H), 3.88-3.66 (m, 2H), 3.45-3.37 (m, 1H), 3.04 (s, 3H), 2.96 (s, 3H), 3.15-2.94 (comp, 2H), 2.49-2.38 (m, 1H), 2.29-2.21 (m, 1H), 2.19-2.08 (m, 1H), 1.95-1.59 (comp, 6H), 1.48 (d, J=6.7 Hz, 3H), 1.44 (d, J=6.7 Hz, 3H); MS (ESI) m/z 574.30 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 5.58-5.38 (m, 1H), 4.67 (s, 2H), 4.11 (s, 1H), 4.03-3.30 (comp, 5H), 3.04 (s, 3H), 2.96 (s, 3H), 3.22-2.94 (comp, 2H), 2.76-2.10 (comp, 4H), 1.72-1.59 (m, 1H); MS (ESI) m/z 550.18 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 1H), 6.94 (s, 1H), 4.14 (s, 2H), 4.11 (s, 1H), 3.16-2.90 (m, 9H), 2.62-2.55 (m, 1H), 2.25-2.20 (m, 1H), 1.65-1.56 (m, 1H), 1.46 (s, 9H); MS (ESI) m/z 500.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.92 (s, 1H), 6.86 (s, 1H), 4.18 (dd, J=43.6 Hz and 13.2 Hz, 2H), 4.04 (s, 1H), 2.97-2.83 (m, 10H), 2.56-2.46 (m, 1H), 2.18-1.14 (m, 1H), 1.57-1.47 (m, 1H), 1.39-1.38 (m, 3H), 0.99-0.97 (m, 1H), 0.72-0.64 (m, 2H), 0.51-0.49 (m, 1H), 0.28-0.26 (m, 1H); MS (ESI) m/z 512.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.97 (s, 1H), 6.91 (s, 1H), 4.15 (s, 2H), 4.10 (s, 1H), 3.15-2.87 (m, 11H), 2.67-2.52 (m, 2H), 2.24-2.16 (m, 1H), 1.98-1.88 (m, 2H), 1.81-1.43 (m, 9H), 1.34-1.20 (m, 2H); MS (ESI) m/z 554.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 1H), 6.94 (s, 1H), 4.33 (s, 2H), 4.09 (s, 1H), 3.57-3.47 (m, 2H), 3.03 (s, 3H), 2.96 (s, 3H), 3.23-2.87 (comp, 5H), 2.64-2.55 (m, 1H), 2.24-1.94 (comp, 5H), 1.66-1.54 (comp, 1H); MS (ESI) m/z 498.19

To a suspension of

oc-amino)isobutyric acid (3.0 g, 14.8 mmol, 1.0 equiv) in 4:1 dichloromethane-dimethylformamide (30 mL) was added hydroxybenzotriazole (2.19 g, 16.2 mmol, 1.1 equiv) and dicyclohexylcarbodiimide (3.26 g, 15.8 mmol, 1.07 equiv). After stirring at rt for 30 min, solution of dimethylamine in THF (2.0 M, 11 mL, 22.1 mmol, 1.5 equiv) was added. The reaction mixture was stirred at rt for another 16 h and then diluted with dichloromethane (100 mL). The reaction solution was washed sequentially with saturated NaHCO₃ (2×30 mL), H₂O (30 mL), citric acid (10% w/v, 2×30 mL), and brine (30 mL). The organic solution was dried over sodium sulfate, and concentrated to give the crude product, which was purified by Biotage to provide the desired dimethylamide as white solid.

The dimethylamide prepared above was dissolved in a solution of HCl in dioxane (4.0 M, 25 mL). The reaction mixture was stirred at rt for an overnight. After evaporating volatiles, the white solid residue was washed with diethylether, and further dried under high vacuum. The formed amine hydrochloric acid salt was used directly for the next step.

To a solution of compound S15-8 (0.10 g, 0.26 mmol, 1.0 equiv) in 1,2-dichloroethane (10 mL) was added amine hydrochloric acid salt (0.131 mg, 0.79 mmol, 3.0 equiv) and triethylamine (0.11 mL, 0.79 mmol, 3.0 equiv). After stirring at rt for 30 min, acetic acid (0.045 mL, 0.79 mmol, 3.0 equiv) was added. After another 24 hrs, sodium triacetoxyborohydride (0.111 g, 0.53 mmol, 2.0 equiv) was added. Stirring was continued for 36 hrs. The reaction mixture was poured into saturated aqueous NaHCO₃ and extracted three times with dichloromethane. The combined organic extracts were washed with brine, dried over sodium sulfate, and concentrated to give the crude intermediate as off-white oil. The crude intermediate was redissolved in 1,2-dichloroethane (2 mL). Formaldehyde (37 wt % in H₂O, 0.044 mL, 0.59 mmol) and acetic acid (0.034 mL, 0.059 mmol) were added. After stirring at rt for 1 h, sodium triacetoxyborohydride (0.083 g, 0.39 mmol) was added. Stirring was continued for another hour. The reaction mixture was poured into saturated aqueous NaHCO₃ and extracted three times with dichloromethane. The combined organic extracts were washed with brine, dried over sodium sulfate, and concentrated. Purification of the residue by Biotage gave compound S15-15-2 as a colorless oil: ¹H NMR (400 MHz, CDCl₃) δ 7.20-7.44 (m, 8H), 7.12 (d, J=7.8 Hz, 2H), 6.98 (s, 1H), 5.14 (s, 2H), 3.61 (s, 2H), 3.41 (s, 3H), 2.89 (s, 3H), 2.46 (s, 3H), 2.04 (s, 3H), 1.38 (s, 6H); MS (ESI) m/z 509.2 (M+H), calcd for C₂₉H₃₄ClN₂O₄ 509.21.

Compound S15-13-227 was prepared from S15-15-2 using similar procedures to that of S15-13-15. The crude product was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 10→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 11.30-14.05 min, were collected and freeze-dried to give the desired product S15-13-227 as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.61 and 7.59 (s, 1H), 4.36 (br s, 2H), 4.13 (s, 1H), 3.42 (dd, J=4.6, 16.0 Hz, 1H), 2.97-3.16 (m, 14H), 2.71 (s, 3H), 2.43 (dd, J=15.1 Hz, 1H), 2.25-2.28 (m, 1H), 1.89 (s, 3H), 1.69 (s, 3H), 1.60-1.70 (m, 1H); MS (ESI) m/z 605.4 (M+H), calcd for C₂₉H₃₈ClN₄O₈ 605.23.

Compound S15-13-228 was prepared using similar procedures to that of S15-13-227, a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.44 (s, 1H), 4.31-4.45 (m, 2H), 4.13 (s, 1H), 3.42 (dd, J=4.6, 16.0 Hz, 1H), 2.97-3.16 (m, 11H), 2.73 (s, 3H), 2.43 (dd, J=15.1 Hz, 1H), 2.25-2.28 (m, 1H), 1.75 (s, 3H), 1.63 (s, 3H), 1.60-1.70 (m, 1H); MS (ESI) m/z 591.5 (M+H), calcd for C₂₈H₃₆ClN₄O₈ 591.21.

Compound S15-13-229 was prepared using similar procedures to that of S15-13-227, a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.46 (s, 1H), 4.35-4.38 (m, 2H), 4.12 (s, 1H), 3.42 (dd, J=4.6, 16.0 Hz, 1H), 2.97-3.16 (m, 8H), 2.73 (s, 3H), 2.43 (dd, J=15.1 Hz, 1H), 2.25-2.28 (m, 1H), 1.78 (s, 3H), 1.66 (s, 3H), 1.60-1.70 (m, 1H); MS (ESI) m/z 577.5 (M+H), calcd for C₂₇H₃₄ClN₄O₈ 577.20.

Compound S15-13-230 was prepared using similar procedures to that of S15-13-227, a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.39 (s, 1H), 4.39 (dd, J=9.6, 12.8 Hz, 1H), 4.23 (dd, J=9.6, 12.8 Hz, 1H), 4.13 (s, 1H), 3.41 (dd, J=4.6, 16.0 Hz, 1H), 2.97-3.16 (m, 8H), 2.71 (s, 3H), 2.40 (dd, J=15.1 Hz, 1H), 2.25-2.28 (m, 1H), 1.78 (s, 3H), 1.62 (s, 3H), 1.60-1.70 (m, 1H), 1.44 (s, 9H); MS (ESI) m/z 633.6 (M+H), calcd for C₃₁H₄₂ClN₄O₈ 633.26.

Compound S15-13-231 was prepared using similar procedures to that of S15-13-227, a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.67 (d, J=7.8 Hz, 2H), 7.53 (s, 1H), 7.38 (t, J=7.8 Hz, 2H), 7.20 (d, J=7.8 Hz, 1H), 4.46 (s, 2H), 4.13 (s, 1H), 3.42 (dd, J=4.6, 16.0 Hz, 1H), 2.97-3.16 (m, 8H), 2.80 (s, 3H), 2.42 (dd, J=15.1 Hz, 1H), 2.25-2.28 (m, 1H), 1.94 (s, 3H), 1.78 (s, 3H), 1.60-1.70 (m, 1H); MS (ESI) m/z 653.5 (M+H), calcd for C₃₃H₃₈ClN₄O₈ 653.23.

Example 16. Synthesis of Compounds Via Scheme 16

The following compounds were prepared according to Scheme 16.

A solution of HNO₃ (68-70%, 0.56 mL, 8.57 mmol, 1.05 equiv) in concentrated H₂SO₄ (2 mL) was added dropwise to a solution of compound S15-4-a (2.00 g, 8.16 mmol, 1.0 equiv) in concentrated H₂SO₄ (20 mL) at 0° C. The reaction mixture was stirred at 0° C. for 10 min and poured onto ice (˜200 mL). The mixture was extracted with EtOAc (150 mL). The organic phase was separated, washed with brine (2×50 mL), dried over magnesium sulfate, filtered, and concentrated to give crude S16-1 as an orange solid: ¹H NMR (400 MHz, CDCl₃) δ 11.5 (br s, 1H), 7.06 (s, 1H), 3.90 (s, 3H), 2.32 (s, 3H); MS (ESI) m/z 288.01, 289.99 (M−H).

Compound S16-1 was dissolved in dichloromethane (16 mL). Oxalyl chloride (0.85 mL, 9.79 mmol, 1.2 equiv) was added, followed by a few drops of DMF. The reaction mixture was stirred at rt for 30 min, concentrated, and further dried under high vacuum. The residue was redissolved in dichloromethane (16 mL). Phenol (0.92 g, 9.79 mmol, 1.2 equiv), triethylamine (2.84 mL, 20.40 mmol, 2.5 equiv), and DMAP (100 mg, 0.82 mmol, 0.1 equiv) were added. The reaction was stirred at rt for 1 h and concentrated under reduced pressure. The residue was dissolved in EtOAc (150 mL), washed with 1 N aqueous HCl (50 mL), brine (50 mL), 1 N aqueous NaOH (50 mL), and then brine (50 mL), dried over anhydrous magnesium sulfate, filtered, and concentrated to afford the desired product S16-2 as a light yellow solid: ¹H NMR (400 MHz, CDCl₃) δ 7.45-7.41 (m, 2H), 7.30-7.26 (m, 1H), 7.21-7.16 (m, 2H), 7.09 (s, 1H), 3.94 (s, 3H), 2.38 (s, 3H); MS (ESI) m/z 364.05, 366.06 (M−H).

A solution of BBr₃ in dichloromethane (1.0 M, 8.16 mL, 8.16 mmol, 1.0 equiv) was added slowly to a solution of compound S16-2 in dichloromethane (32 mL) at −78° C. The reaction was stirred at −78° C. for 15 min and then allowed to warm to 0° C. in 50 min and kept at that temperature for 10 min. The reaction mixture was poured into saturated aqueous NaHCO₃ solution (50 mL) and stirred at rt for 10 min. The dichloromethane was evaporated. The residue was extracted with EtOAc (100 mL, then 30 mL). The organic extracts were combined and dried over anhydrous magnesium sulfate. The dried solution was filtered, and the filtrate was concentrated to give crude S16-3 (2.20 g): ¹H NMR (400 MHz, CDCl₃) δ 11.2 (br s, 1H), 7.48-7.44 (m, 2H), 7.36-7.32 (m, 1H), 7.25 (s, 1H), 7.18-7.16 (m, 2H), 2.63 (s, 3H); MS (ESI) m/z 350.01, 352.03 (M−H).

Benzylbromide (0.78 mL, 6.56 mmol, 1.05 equiv) and K₂CO₃ powder (1.73 g, 12.50 mmol, 2.0 equiv) were added to a solution of compound S16-3 (2.20 g, 6.25 mmol, 1.0 equiv) in acetone (12 mL). The mixture was stirred at rt overnight. The solid was filtered off and further washed with EtOAc (30 mL). The filtrate was concentrated. The residue was purified by flash column chromatography (2-20% EtOAc/hexanes) to afford the desired product S16-4 as a white solid (1.68 g, 47% over four steps): ¹H NMR (400 MHz, CDCl₃) δ 7.40-7.32 (m, 8H), 7.15 (s, 1H), 7.03-7.01 (m, 2H), 5.18 (s, 2H), 2.39 (s, 3H); MS (ESI) m/z 440.09, 442.06 (M−H).

Zn dust (2.33 g, 35.70 mmol, 10.0 equiv) was added portionwise to a solution of compound S16-4 (1.58 g, 3.57 mmol, 1.0 equiv) in a mixture of THF (5 mL) and HOAc (1 mL) (caution: exothermic!). The reaction mixture was stirred at rt for 3.5 hrs, diluted with EtOAc, and filtered through a pad of Celite. The cake was washed thoroughly with EtOAc. The filtrate was washed with saturated aqueous NaHCO₃ (60 mL). The aqueous layer was extracted with EtOAc (40 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to yield the crude aniline intermediate S16-4a, which was used directly in the next step.

HCHO (1.59 mL, 37% aqueous solution, 21.42 mmol, 6.0 equiv), HOAc (0.62 mL, 10.71 mmol, 3.0 equiv) and Na(OAc)₃BH (2.27 g, 10.71 mmol, 3.0 equiv) were added to a solution of the above crude product S16-4a in acetonitrile (30 mL). The reaction mixture was then stirred at rt for 2 h. Then Na(OAc)₃BH (0.38 g, 1.78 mmol, 0.5 equiv) was added. The reaction mixture was stirred at rt overnight. Saturated aqueous NaHCO₃ (70 mL) was added slowly (bubbling). The resulting mixture was then stirred at rt for 5 min and extracted with EtOAc (100 mL, then 50 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated. The residue was purified by flash column chromatography (2-5% EtOAc/hexanes) to afford the desired product S16-5 as a white solid (1.43 g, 91% over 2 steps): ¹H NMR (400 MHz, CDCl₃) δ 7.44-7.35 (m, 7H), 7.26-7.22 (m, 1H), 7.09-7.07 (m, 3H), 5.10 (s, 2H), 2.83 (s, 6H), 2.42 (s, 3H); MS (ESI) m/z 440.12, 442.13 (M+H).

n-BuLi (1.46 mL, 2.17 M/hexanes, 3.16 mmol, 1.10 equiv) was added dropwise to a solution of diisopropylamine (0.45 mL, 3.16 mmol, 1.10 equiv) in THF (6 mL) at −78° C. The reaction solution was warmed to −20° C. and then re-cooled to −78° C. TMEDA (0.47 mL, 3.16 mmol, 1.10 equiv) was added and the reaction mixture was stirred at −78° C. for 15 min. A solution of ester S16-5 (1.27 g, 2.88 mmol, 1.05 equiv) in THF (3 mL) was added via a cannula. The resulting deep red solution was stirred at −78° C. for 55 min and was then cooled to −100° C. A solution of enone S1-9 (1.33 g, 2.75 mmol, 1.0 equiv) in THF (3 mL) was added to the reaction mixture via a cannula. The reaction mixture was allowed to warm to −70° C. over 30 min. LHMDS (3.02 mL, 1.0 M/THF, 3.02 mmol, 1.1 equiv) was added. The reaction mixture was warmed to −5° C. slowly over 1 h 20 min, quenched by a mixture of saturated aqueous NH₄Cl and pH 7 phosphate buffer (100 mL, 1:1, v/v), and extracted with EtOAc (75 mL, then 25 mL). The combined EtOAc extracts were dried (sodium sulfate), filtered and concentrated. The residue was purified by a preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 4.0 mL (CH₃CN), gradient: 80→100% B in A over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 7.6-11 min, were collected and concentrated at rt to remove most of the acetonitrile. The resulting mostly aqueous solution was freeze-dried to yield the desired product S16-6 (1.71 g, 75%): ¹H NMR (400 MHz, CDCl₃) δ 16.00 (br s, 1H), 7.50-7.46 (m, 4H), 7.39-7.27 (m, 6H), 7.10 (s, 1H), 5.36 (s, 2H), 5.18, 5.12 (ABq, J=12.8 Hz, 2H), 4.10 (d, J=10.4 Hz, 1H), 3.37 (dd, J=4.3, 15.9 Hz, 1H), 2.88-2.74 (m, 7H), 2.55-2.40 (m, 9H), 2.12 (d, J=14.0 Hz, 1H), 0.84 (s, 9H), 0.28 (s, 3H), 0.14 (s, 3H); MS (ESI) m/z 828.27, 830.30 (M+H).

A solution of phenyllithium in di-n-butyl ether (0.10 mL, 1.8 M, 0.18 mmol, 1.5 equiv) was added dropwise to a solution of compound S16-6 (0.10 g, 0.12 mmol, 1.0 equiv) in THF (3 mL) at −78° C., forming an orange solution. After 5 min, a solution of n-butyllithium in hexanes (68 μL, 2.2 M, 0.15 mmol, 1.2 equiv) was added dropwise at −78° C., followed 2 min later by the addition of N,N-dimethylformamide (47 μL, 0.61 mmol, 5.0 equiv). The resulting dark red reaction mixture was stirred at −78° C. for 65 min. Saturated aqueous ammonium chloride (10 mL) was added dropwise at −78° C. The reaction mixture was allowed to warm to 23° C., diluted with saturated aqueous ammonium chloride (−20 mL), and extracted with EtOAc (2×15 mL). The organic extracts were combined, dried over anhydrous sodium sulfate, filtered, and concentrated, affording compound S16-7 as an orange oil, which was used directly in the next reactions: ¹H NMR (400 MHz, CDCl₃) δ 15.85 (s, 1H), 10.32 (s, 1H), 7.50-7.46 (m, 4H), 7.39-7.26 (m, 7H), 5.36 (s, 2H), 5.25, 5.18 (ABq, J=12.8 Hz, 2H), 4.02 (d, J=11.0 Hz, 1H), 3.13 (dd, J=4.9, 15.9 Hz, 1H), 2.98-2.90 (m, 7H), 2.58-2.46 (m, 9H), 2.15 (d, J=14.0 Hz, 1H), 0.83 (s, 9H), 0.27 (s, 3H), 0.15 (s, 3H); MS (ESI) m/z 778.34 (M+H).

t-Butylamine (19 μL, 0.18 mmol, 3.0 equiv), acetic acid (10 μL, 0.18 mmol, 3.0 equiv) and sodium triacetoxyborohydride (26 mg, 0.12 mmol, 2.0 equiv) were added sequentially to a solution of compound S16-7 (half of the above crude product, 0.061 mmol, 1.0 equiv) in 1,2-dichloroethane (2 mL) at 23° C. After stirring for 1 h 45 min, the reaction mixture was quenched by the addition of saturated aqueous sodium bicarbonate and brine (1:1, 15 mL) and extracted with dichloromethane (2×15 mL). The combined organic extracts were dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 3.0 mL (CH₃CN); gradient: 20→100% B in A over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 5.6-6.5 min, were collected and freeze-dried to yield compound S16-8-1 (19 mg, 37% for 2 steps): ¹H NMR (400 MHz, CDCl₃) δ 7.50-7.48 (m, 4H), 7.39-7.26 (m, 6H), 7.06 (s, 1H), 5.35 (s, 2H), 5.23, 5.17 (ABq, J=12.8 Hz, 2H), 4.04 (d, J=10.4 Hz, 1H), 3.81, 3.63 (ABq, J=13.4 Hz, 2H), 3.09 (dd, J=4.3, 15.3 Hz, 1H), 2.88-2.76 (m, 7H), 2.53-2.46 (m, 9H), 2.11 (d, J=13.4 Hz, 1H), 1.14 (s, 9H), 0.83 (s, 9H), 0.27 (s, 3H), 0.14 (s, 3H); MS (ESI) m/z 835.48 (M+H).

Aqueous HF (48-50%, 0.2 mL) was added to a solution of compound S16-9-1 (19 mg, 0.024 mmol, 1.0 equiv) in acetonitrile (0.6 mL) in a polypropylene reaction vessel at 23° C. The mixture was stirred vigorously at 23° C. overnight and poured into aqueous K₂HPO₄ (2.5 g dissolved in 20 mL water). The mixture was extracted with EtOAc (2×25 mL). The combined organic extracts were dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was used directly in the next step without further purification.

Pd—C (10 wt %, 9 mg) was added in one portion into the yellow solution of the above crude product in a mixture of HCl/MeOH (0.5 N, 0.14 mL, 3.0 equiv) and MeOH (2 mL) at 23° C. The reaction vessel was sealed and purged with hydrogen by briefly evacuating the flask followed by flushing with hydrogen gas (1 atm). The resulting mixture was stirred at 23° C. for 30 min. The reaction mixture was then filtered through a small Celite pad. The filtrate was concentrated. The residue was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 15→60% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 5.8-8.0 min, were collected and freeze-dried to yield compound S16-10-1 (11 mg, 67% for 2 steps): ¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.36 (d, J=12.8 Hz, 1H), 4.14 (d, J=12.8 Hz, 1H), 4.12 (s, 1H), 3.11-2.83 (m, 15H), 2.45 (t, J=14.2 Hz, 1H), 2.29-2.26 (m, 1H), 1.70-1.60 (m, 1H), 1.47 (s, 9H); MS (ESI) m/z 543.31 (M+H).

The following compounds were prepared similarly to S16-8-1 or S16-10-1.

S16-8-2:

¹H NMR (400 MHz, CDCl₃) δ 7.44-7.41 (m, 4H), 7.33-7.23 (m, 6H), 7.11 (s, 1H), 5.29 (s, 2H), 5.23, 5.15 (ABq, J=12.8 Hz, 2H), 4.07 (d, J=14.0 Hz, 1H), 3.96 (d, J=10.4 Hz, 1H), 3.80 (d, J=14.0 Hz, 1H), 2.96 (dd, J=4.3, 15.9 Hz, 1H), 2.83-2.71 (m, 8H), 2.51-2.36 (m, 11H), 2.05 (d, J=14.6 Hz, 1H), 0.77 (s, 9H), 0.21 (s, 3H), 0.08 (s, 3H); MS (ESI) m/z 793.35 (M+H).

S16-8-3:

¹H NMR (400 MHz, CDCl₃) δ 7.50-7.47 (m, 4H), 7.39-7.28 (m, 6H), 7.15 (s, 1H), 5.35 (s, 2H), 5.28, 5.21 (ABq, J=12.8 Hz, 2H), 4.11 (d, J=14.0 Hz, 1H), 4.02 (d, J=10.4 Hz, 1H), 3.84 (d, J=14.0 Hz, 1H), 3.02 (dd, J=4.9, 15.9 Hz, 1H), 2.88-2.76 (m, 8H), 2.66 (q, J=7.3 Hz, 2H), 2.56-2.44 (m, 8H), 2.11 (d, J=14.0 Hz, 1H), 1.19 (t, J=7.3 Hz, 3H), 0.83 (s, 9H), 0.27 (s, 3H), 0.14 (s, 3H); MS (ESI) m/z 807.37 (M+H).

S16-8-4:

¹H NMR (400 MHz, CDCl₃) δ 7.50-7.47 (m, 4H), 7.39-7.31 (m, 5H), 7.28-7.24 (m, 1H), 7.00 (s, 1H), 5.35 (s, 2H), 5.23, 5.18 (ABq, J=12.2 Hz, 2H), 4.05 (d, J=10.4 Hz, 1H), 3.88, 3.65 (ABq, J=14.6 Hz, 2H), 3.10 (dd, J=4.3, 15.9 Hz, 1H), 2.93-2.74 (m, 7H), 2.54-2.44 (m, 9H), 2.33 (d, J=6.7 Hz, 2H), 2.12 (d, J=14.0 Hz, 1H), 1.76-1.69 (m, 1H), 0.88 (d, J=6.7 Hz, 6H), 0.84 (s, 9H), 0.28 (s, 3H), 0.15 (s, 3H); MS (ESI) m/z 835.50 (M+H).

S16-8-5:

¹H NMR (400 MHz, CDCl₃) δ 7.50-7.47 (m, 4H), 7.39-7.24 (m, 6H), 7.21 (s, 1H), 5.35 (s, 2H), 5.24, 5.19 (ABq, J=12.8 Hz, 2H), 4.06-3.99 (m, 2H), 3.76-3.73 (m, 1H), 3.08 (dd, J=4.3, 15.3 Hz, 1H), 2.89-2.71 (m, 8H), 2.55-2.46 (m, 8H), 2.31-2.26 (m, 2H), 2.11 (d, J=13.4 Hz, 1H), 0.92 (s, 9H), 0.83 (s, 9H), 0.27 (s, 3H), 0.14 (s, 3H); MS (ESI) m/z 849.40 (M+H).

S16-8-6:

¹H NMR (400 MHz, CDCl₃) δ 7.50-7.48 (m, 4H), 7.39-7.31 (m, 5H), 7.28-7.24 (m, 1H), 7.08 (s, 1H), 5.35 (s, 2H), 5.23, 5.19 (ABq, J=12.8 Hz, 2H), 4.05 (d, J=10.4 Hz, 1H), 3.73, 3.57 (ABq, J=13.4 Hz, 2H), 3.10 (dd, J=4.3, 15.3 Hz, 1H), 2.92-2.76 (m, 7H), 2.53-2.45 (m, 9H), 2.11 (d, J=13.4 Hz, 1H), 1.44 (q, J=7.3 Hz, 2H), 1.06 (s, 6H), 0.86 (t, J=7.3 Hz, 3H), 0.84 (s, 9H), 0.28 (s, 3H), 0.14 (s, 3H); MS (ESI) m/z 849.43 (M+H).

S16-8-7:

¹H NMR (400 MHz, CDCl₃) δ 7.50-7.48 (m, 4H), 7.39-7.31 (m, 5H), 7.28-7.26 (m, 1H), 7.08 (s, 1H), 5.35 (s, 2H), 5.24, 5.18 (ABq, J=12.8 Hz, 2H), 4.05 (d, J=10.4 Hz, 1H), 3.90, 3.71 (ABq, J=13.4 Hz, 2H), 3.11 (dd, J=4.3, 15.9 Hz, 1H), 2.92-2.76 (m, 7H), 2.54-2.43 (m, 9H), 2.11 (d, J=14.0 Hz, 1H), 0.98 (d, J=2.4 Hz, 6H), 0.83 (s, 9H), 0.39-0.34 (m, 2H), 0.27 (s, 3H), 0.25-0.22 (m, 2H), 0.14 (s, 3H); MS (ESI) m/z 861.39 (M+H).

S16-8-8:

¹H NMR (400 MHz, CDCl₃) δ 16.11 (br s, 1H), 7.51-7.48 (m, 4H), 7.39-7.30 (m, 6H), 7.26 (s, 1H), 5.35 (s, 2H), 5.28, 5.21 (ABq, J=12.8 Hz, 2H), 4.04 (d, J=10.4 Hz, 1H), 3.57 (br s, 2H), 3.12 (dd, J=3.7, 15.3 Hz, 1H), 2.92-2.72 (m, 7H), 2.54-2.43 (m, 9H), 2.26 (s, 6H), 2.11 (d, J=1.34 Hz, 1H), 0.83 (s, 9H), 0.27 (s, 3H), 0.14 (s, 3H); MS (ESI) m/z 807.32 (M+H).

S16-8-9:

¹H NMR (400 MHz, CDCl₃) δ 16.12 (br s, 1H), 7.50-7.46 (m, 4H), 7.39-7.28 (m, 6H), 7.23 (s, 1H), 5.35 (s, 2H), 5.26 (s, 2H), 4.05 (d, J=10.4 Hz, 1H), 3.58 (br s, 2H), 3.15-3.12 (m, 1H), 2.92-2.85 (m, 1H), 2.77-2.70 (m, 7H), 2.53-2.43 (m, 9H), 2.31-2.27 (m, 2H), 2.12-2.04 (m, 3H), 0.88 (s, 9H), 0.84 (s, 9H), 0.28 (s, 3H), 0.15 (s, 3H); MS (ESI) m/z 863.44 (M+H).

S16-8-10:

¹H NMR (400 MHz, CDCl₃) δ 16.04 (br s, 1H), 7.40-7.38 (m, 2H), 7.34-7.32 (m, 2H), 7.29-7.19 (m, 5H), 7.15-7.14 (m, 1H), 7.11 (s, 1H), 5.26 (s, 2H), 5.12 (s, 2H), 3.97 (d, J=10.4 Hz, 1H), 3.76 (d, J=15.9 Hz, 1H), 3.58 (d, J=15.9 Hz, 1H), 3.04 (dd, J=4.3, 15.9 Hz, 1H), 2.84-2.78 (m, 1H), 2.69 (s, 3H), 2.62 (s, 3H), 2.43-2.28 (m, 11H), 2.02 (d, J=14.0 Hz, 1H), 1.76-1.75 (m, 1H), 0.75 (s, 9H), 0.73 (s, 9H), 0.18 (s, 3H), 0.06 (s, 3H), 0.03-0.09 (m, 4H); MS (ESI) m/z 899.48 (M+H).

S16-8-11:

¹H NMR (400 MHz, CDCl₃) δ 16.14 (br s, 1H), 7.50-7.45 (m, 4H), 7.39-7.31 (m, 5H), 7.25-7.21 (m, 2H), 5.36 (s, 2H), 5.22 (s, 2H), 4.05 (d, J=10.4 Hz, 1H), 3.53, 3.46 (ABq, J=15.3 Hz, 2H), 3.18 (dd, J=3.7, 15.3 Hz, 1H), 2.92-2.86 (m, 1H), 2.81-2.71 (m, 6H), 2.53-2.31 (m, 11H), 2.11 (d, J=13.4 Hz, 1H), 1.64 (br s, 4H), 1.62 (s, 3H), 0.98 (s, 3H), 0.84 (s, 9H), 0.28 (s, 3H), 0.15 (s, 3H); MS (ESI) m/z 861.49 (M+H).

S16-10-2:

¹H NMR (400 MHz, CD₃OD) δ 6.89 (s, 1H), 4.37 (d, J=13.7 Hz, 1H), 4.12 (d, J=13.7 Hz, 1H), 4.10 (s, 1H), 3.10-2.78 (m, 18H), 2.43 (t, J=14.6 Hz, 1H), 2.27-2.24 (m, 1H), 1.70-1.60 (m, 1H); MS (ESI) m/z 501.29 (M+H).

S16-10-3:

¹H NMR (400 MHz, CD₃OD) δ 6.94 (s, 1H), 4.38 (d, J=13.7 Hz, 1H), 4.15 (d, J=14.2 Hz, 1H), 4.11 (s, 1H), 3.16 (q, J=7.3 Hz, 2H), 3.12-2.81 (m, 15H), 2.44 (t, J=14.2 Hz, 1H), 2.28-2.25 (m, 1H), 1.69-1.60 (m, 1H), 1.37 (t, J=7.3 Hz, 3H); MS (ESI) m/z 515.30 (M+H).

S16-10-4:

¹H NMR (400 MHz, CD₃OD) δ 7.05 (s, 1H), 4.50 (d, J=13.3 Hz, 1H), 4.26 (d, J=13.7 Hz, 1H), 4.15 (s, 1H), 3.18-2.89 (m, 17H), 2.52 (t, J=12.8 Hz, 1H), 2.34-2.31 (m, 1H), 2.15-2.08 (m, 1H), 1.70-1.61 (m, 1H), 1.06 (d, J=6.4 Hz, 6H); MS (ESI) m/z 543.32 (M+H).

S16-10-5:

¹H NMR (400 MHz, CD₃OD) δ 6.93 (s, 1H), 4.42 (d, J=13.7 Hz, 1H), 4.17 (d, J=13.7 Hz, 1H), 4.10 (s, 1H), 3.04-2.83 (m, 17H), 2.47 (t, J=14.6 Hz, 1H), 2.28-2.24 (m, 1H), 1.70-1.60 (m, 1H), 1.08 (s, 9H); MS (ESI) m/z 557.31 (M+H).

S16-10-6:

¹H NMR (400 MHz, CD₃OD) δ 7.03 (s, 1H), 4.38 (d, J=12.8 Hz, 1H), 4.16 (d, J=12.8 Hz, 1H), 4.14 (s, 1H), 3.13-2.87 (m, 15H), 2.47 (t, J=14.6 Hz, 1H), 2.31-2.28 (m, 1H), 1.82 (q, J=7.3 Hz, 2H), 1.70-1.61 (m, 1H), 1.44 (s, 6H), 1.05 (t, J=7.3 Hz, 3H); MS (ESI) m/z 557.32 (M+H).

S16-10-7:

¹H NMR (400 MHz, CD₃OD) δ 7.04 (s, 1H), 4.50 (d, J=13.3 Hz, 1H), 4.26 (d, J=13.3 Hz, 1H), 4.14 (s, 1H), 3.12-2.86 (m, 15H), 2.46 (t, J=14.2 Hz, 1H), 2.31-2.27 (m, 1H), 1.70-1.61 (m, 1H), 1.34 (s, 6H), 1.25-1.19 (m, 1H), 0.72-0.59 (m, 4H); MS (ESI) m/z 569.30 (M+H).

S16-10-8:

¹H NMR (400 MHz, CD₃OD) δ 6.96 (s, 1H), 4.55 (d, J=13.3 Hz, 1H), 4.23 (d, J=13.3 Hz, 1H), 4.12 (s, 1H), 3.09-2.82 (m, 21H), 2.47 (t, J=15.1 Hz, 1H), 2.28-2.25 (m, 1H), 1.70-1.60 (m, 1H); MS (ESI) m/z 515.28 (M+H).

S16-10-9:

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 1H), 4.74 (d, J=12.8 Hz, 0.7H), 4.54 (br d, J=12.8 Hz, 0.3H), 4.38 (br d, J=12.8 Hz, 0.3H), 4.17 (d, J=12.8 Hz, 0.3H), 4.11 (s, 1H), 3.20-2.80 (m, 20H), 2.55-2.48 (m, 1H), 2.29-2.26 (m, 1H), 1.70-1.61 (m, 1H), 1.10 (s, 9H); MS (ESI) m/z 571.38 (M+H).

S16-10-10:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (s, 1H), 4.72 (d, =12.8 Hz, 1H), 4.42 (d, J=12.8 Hz, 1H), 4.10 (s, 1H), 3.09-2.85 (m, 18H), 2.51 (t, J=15.6 Hz, 1H), 2.28-2.24 (m, 1H), 1.71-1.62 (m, 1H), 1.13 (s, 9H), 1.08-1.06 (m, 4H); MS (ESI) m/z 597.38 (M+H).

S16-10-11:

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 0.4H), 6.97 (s, 0.6H), 4.68 (d, J=12.8 Hz, 0.6H), 4.43 (d, J=12.8 Hz, 0.4H), 4.19 (d, J=12.8 Hz, 0.4H), 4.10 (s, 1H), 3.93 (d, J=12.8 Hz, 0.6H), 3.08-2.80 (m, 17H), 2.48 (t, J=16.0 Hz, 1H), 2.28-2.24 (m, 1H), 2.16-2.14 (m, 2H), 2.04-1.99 (m, 2H), 1.71-1.61 (m, 1H), 1.61 (s, 3H), 1.44 (s, 3H); MS (ESI) m/z 569.32 (M+H).

S16-10-12:

¹H NMR (400 MHz, CD₃OD) δ 6.96 (s, 1H), 4.81 (dt, J=47.2, 4.1 Hz, 2H), 4.49 (d, J=14.2 Hz, 1H), 4.26 (d, J=14.2 Hz, 1H), 4.13 (s, 1H), 3.49 (dt, J=26.6, 3.7 Hz, 2H), 3.12-2.85 (m, 15H), 2.46 (t, J=14.2 Hz, 1H), 2.30-2.27 (m, 1H), 1.70-1.60 (m, 1H); MS (ESI) m/z 533.31 (M+H).

S16-10-13:

¹H NMR (400 MHz, CD₃OD) δ 6.94 (s, 1H), 6.37 (tt, J=2.8, 53.6 Hz, 1H), 4.52 (d, J=14.2 Hz, 1H), 4.29 (d, J=14.2 Hz, 1H), 4.13 (s, 1H), 3.64 (t, J=15.6 Hz, 2H), 3.12-2.85 (m, 15H), 2.47 (t, J=14.2 Hz, 1H), 2.31-2.27 (m, 1H), 1.70-1.60 (m, 1H); MS (ESI) m/z 551.27 (M+H).

S16-10-14:

¹H NMR (400 MHz, CD₃OD) δ 6.95 (s, 1H), 4.42 (d, J=14.2 Hz, 1H), 4.19 (d, J=14.2 Hz, 1H), 4.12 (s, 1H), 3.07-2.97 (m, 17H), 2.44 (t, J=14.2 Hz, 1H), 2.28-2.25 (m, 1H), 1.70-1.60 (m, 1H), 1.21-1.14 (m, 1H), 0.77-0.73 (m, 2H), 0.46-0.42 (m, 2H); MS (ESI) m/z 541.29 (M+H).

S16-10-15:

¹H NMR (400 MHz, CD₃OD) δ 6.93 (s, 1H), 4.39 (d, J=13.7 Hz, 1H), 4.14 (d, J=13.7 Hz, 1H), 4.10 (s, 1H), 3.10-2.81 (m, 17H), 2.45 (t, J=14.6 Hz, 1H), 2.28-2.24 (m, 1H), 1.84-1.64 (m, 6H), 1.39-1.21 (m, 4H), 1.10-1.04 (m, 2H); MS (ESI) m/z 583.34 (M+H).

S16-10-16:

¹H NMR (400 MHz, CD₃OD) δ 6.93 (s, 1H), 4.40 (d, J=13.7 Hz, 1H), 4.16 (d, J=13.7 Hz, 1H), 4.12 (s, 1H), 3.10-2.77 (m, 17H), 2.47 (t, J=14.6 Hz, 1H), 2.29-2.26 (m, 1H), 2.04 (br s, 3H), 1.82-1.70 (m, 7H), 1.66-1.64 (m, 6H); MS (ESI) m/z 635.34 (M+H).

S16-10-17:

¹H NMR (400 MHz, CD₃OD) δ 6.93 (s, 1H), 4.49 (d, J=13.7 Hz, 1H), 4.27 (d, J=13.7 Hz, 1H), 4.10 (s, 1H), 3.10-2.81 (m, 16H), 2.43 (t, J=14.2 Hz, 1H), 2.27-2.24 (m, 1H), 1.69-1.60 (m, 1H), 0.95-0.94 (m, 4H); MS (ESI) m/z 527.35

S16-10-18:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (s, 1H), 4.44 (d, J=13.3 Hz, 1H), 4.16 (d, J=13.7 Hz, 1H), 4.12 (s, 1H), 3.45 (s, 1H), 3.08-2.85 (m, 15H), 2.48 (t, J=14.6 Hz, 1H), 2.30-2.23 (m, 3H), 2.06-1.94 (m, 6H), 1.85-1.79 (m, 6H), 1.73-1.61 (m, 1H); MS (ESI) m/z 621.35 (M+H).

S16-10-19:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (s, 1H), 4.64 (d, J=13.3 Hz, 0.5H), 4.48 (d, J=13.3 Hz, 0.5H), 4.32 (d, J=13.3 Hz, 0.5H), 4.12 (s, 1H), 4.11 (d, J=12.8 Hz, 0.5H), 3.22-2.82 (m, 20H), 2.47 (t, J=14.6 Hz, 1H), 2.29-2.25 (m, 1H), 1.70-1.61 (m, 1H), 1.44-1.37 (m, 3H); MS (ESI) m/z 529.30 (M+H).

S16-10-20:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (s, 0.4H), 6.96 (s, 0.6H), 4.86 (br s, 0.4H), 4.72 (d, J=13.3 Hz, 0.6H), 4.43 (br s, 0.6H), 4.13-4.60 (m, 1.4H), 3.25-2.87 (m, 20H), 2.51-2.47 (m, 1H), 2.27 (br s, 2H), 1.66 (br s, 1H), 1.08 (d, J=4.1 Hz, 6H); MS (ESI) m/z 557.32 (M+H).

S16-10-21:

¹H NMR (400 MHz, CD₃OD) δ 6.96 (s, 0.4H), 6.94 (s, 0.6H), 4.72 (d, J=13.3 Hz, 0.4H), 4.42 (s, 0.6H), 4.09 (s, 1H), 4.09 (d, J=13.3 Hz, 0.4H), 3.08-2.81 (m, 20H), 2.50-2.38 (m, 1H), 2.27-2.24 (m, 1H), 1.71-1.57 (m, 5H), 1.38-1.28 (m, 4H); MS (ESI) m/z 583.38 (M+H).

S16-10-22:

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 0.4H), 6.69 (s, 0.6H), 4.81 (d, J=13.3 Hz, 0.6H), 4.54 (d, J=12.8 Hz, 0.4H), 4.24 (d, J=12.8 Hz, 0.4H), 4.12 (s, 1H), 3.96 (d, J=13.3 Hz, 0.6H), 3.05-2.72 (m, 18H), 2.54-2.47 (m, 1H), 2.30-2.26 (m, 1H), 1.71-1.62 (m, 1H), 1.55 (s, 9H); MS (ESI) m/z 557.31 (M+H).

S16-10-23:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (s, 0.35H), 6.95 (s, 0.75H), 4.92 (d, J=13.3 Hz, 0.75H), 4.56 (d, J=12.8 Hz, 0.35H), 4.24 (d, J=12.8 Hz, 0.35H), 4.13 (s, 1H), 4.00 (d, J=13.3 Hz, 0.75H), 3.12-2.73 (m, 18H), 2.55-2.46 (m, 1H), 2.31-2.28 (m, 1H), 2.02-1.82 (m, 2H), 1.71-1.61 (m, 1H), 1.53-1.49 (m, 6H), 1.09 (t, J=7.3 Hz, 3H); MS (ESI) m/z 571.31 (M+H).

S16-10-24:

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 0.4H), 6.96 (s, 0.6H), 4.68 (d, J=12.8 Hz, 0.4H), 4.32 (d, J=12.8 Hz, 0.4H), 4.13 (s, 1H), 4.05 (d, J=13.3 Hz, 0.6H), 3.25-2.80 (m, 18H), 2.55-2.43 (m, 1H), 2.32-2.28 (m, 1H), 1.71-1.62 (m, 1H), 1.42-1.38 (m, 6H), 1.31-1.28 (m, 1H), 0.80-0.78 (m, 2H), 0.68-0.64 (m, 2H); MS (ESI) m/z 583.32 (M+H).

S16-10-25:

¹H NMR (400 MHz, CD₃OD) δ7.02 (s, 1H), 4.71 (d, J=13.3 Hz, 1H), 4.42 (d, J=13.3 Hz, 1H), 4.12 (s, 1H), 3.14-2.82 (m, 18H), 2.49 (t, J=15.6 Hz, 1H), 2.40-2.33 (m, 1H), 2.29-2.26 (m, 1H), 1.70-1.61 (m, 1H), 1.07-0.99 (m, 10H); MS (ESI) m/z 583.36 (M+H).

S16-10-26:

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 5.46 (br d, J=52.2 Hz, 1H), 4.72 (d, J=13.3 Hz, 1H), 4.39 (br s, 1H), 4.12 (s, 1H), 3.85 (br s, 2H), 3.55-3.46 (m, 2H), 3.12-2.84 (m, 15H), 2.51-2.43 (m, 3H), 2.30-2.26 (m, 1H), 1.70-1.60 (m, 1H); MS (ESI) m/z 559.26 (M+H).

S16-10-27:

¹H NMR (400 MHz, CD₃OD) δ 7.04 (s, 1H), 4.56 (d, J=12.8 Hz, 1H), 4.30 (d, J=12.8 Hz, 1H), 4.12 (s, 1H), 3.36-2.83 (m, 19H), 2.47 (t, J=14.2 Hz, 1H), 2.29-2.26 (m, 1H), 1.77-1.61 (m, 5H), 1.11 (s, 3H), 1.05 (s, 3H); MS (ESI) m/z 583.36 (M+H).

S16-10-28:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.58 (d, J=14.0 Hz, 1H), 4.24 (d, J=13.2 Hz, 1H), 4.13 (s, 1H), 3.28-3.24 (m, 4H), 3.09-2.80 (m, 15H), 2.60-2.48 (m, 1H), 2.32-2.28 (m, 1H), 1.68-1.63 (m, 1H), 0.93-0.85 (m, 6H); MS (ESI) m/z 543.1 (M+H).

S16-10-29:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (s, 1H), 5.00-4.95 (m, 1H), 4.85-4.800 (m, 1H), 4.78-4.58 (m, 1H), 4.48-4.24 (m, 1H), 4.14 (s, 1H), 3.68-3.49 (m, 2H), 3.12-2.87 (m, 18H), 2.61-2.49 (m, 1H), 2.32-2.29 (m, 1H), 1.69-1.63 (m, 1H); MS (ESI) m/z 547.0 (M+H).

S16-10-30:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 5.08-5.02 (m, 1H), 4.90-4.85 (m, 1H), 4.76-4.72 (m, 1H), 4.39-4.36 (m, 1H), 4.15 (s, 1H), 3.67-3.60 (m, 2H), 3.16-2.91 (m, 17H), 2.62-2.48 (m, 1H), 2.34-2.31 (m, 1H), 1.72-1.69 (m, 1H), 1.49-1.38 (m, 3H); MS (ESI) m/z 561.1 (M+H).

S16-10-31:

¹H NMR (400 MHz, CD₃OD) δ 6.92 (s, 1H), 6.40 (t, J=54.0 Hz, 1H), 4.56 (d, J=13.2 Hz, 1H), 4.21 (d, J=13.6 Hz, 1H), 4.12 (s, 1H), 3.40-3.57 (m, 3H), 3.21-2.90 (m, 16H), 2.70-2.62 (m, 1H), 2.39-2.27 (m, 1H), 1.76-1.58 (m, 1H); MS (ESI) m/z 565.1 (M+H).

S16-10-32:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (s, 1H), 6.42 (t, J=54.0 Hz, 1H), 4.60 (d, J=15.2 Hz, 1H), 4.30 (d, J=15.2 Hz, 1H), 4.16 (s, 1H), 3.63-3.52 (m, 2H), 3.24-3.00 (m, 17H), 2.66-2.52 (m, 1H), 2.39-2.36 (m, 1H), 1.71-1.65 (m, 1H), 1.31 (t, J=6.8 Hz, 3H); MS (ESI) m/z 579.1 (M+H).

S16-10-33:

¹H NMR (400 MHz, CD₃OD) δ 6.90 (s, 1H), 4.45 (d, J=15.2 Hz, 1H), 4.26 (d, J=15.2 Hz, 1H), 4.12 (s, 1H), 3.81 (m, 2H), 3.20-2.96 (m, 15H), 2.64-2.52 (m, 1H), 2.36-2.31 (m, 1H), 1.69-1.55 (m, 1H); MS (ESI) m/z 569.2 (M+H).

S16-10-34:

¹H NMR (400 MHz, CD₃OD) δ 6.89 (s, 1H), 4.43 (d, J=15.2 Hz, 1H), 4.14 (s, 1H), 4.07 (d, J=15.2 Hz, 1H), 3.64-3.54 (m, 2H), 3.42 (s, 3H), 3.31 (s, 3H), 3.19-2.94 (m, 9H), 2.63 (t, J=28.8, 14.4 Hz, 1H), 2.60 (s, 3H), 2.41-2.37 (m, 1H), 1.69-1.59 (m, 1H); MS (ESI) m/z 583.1 (M+H).

S16-10-35:

¹H NMR (400 MHz, CD₃OD) δ 6.95 (s, 1H), 4.43 (d, J=15.6 Hz, 1H), 4.18 (d, J=15.6 Hz, 1H), 4.16 (s, 1H), 3.66-3.53 (m, 2H), 3.47 (s, 3H), 3.35 (s, 3H), 3.19-2.82 (m, 11H), 2.68-2.55 (m, 1H), 2.42-2.39 (m, 1H), 1.70-1.62 (m, 1H), 1.17 (t, J=7.2 Hz, 3H); MS (ESI) m/z 597.1 (M+H).

S16-10-36:

¹H NMR (400 MHz, CD₃OD) δ 6.90 (s, 1H), 4.35 (d, J=14.0 Hz, 1H), 4.12 (d, J=14.0 Hz, 1H), 4.08 (s, 1H), 3.08-2.75 (m, 17H), 2.48-2.38 (m, 1H), 2.28-2.23 (m, 1H), 1.78-1.73 (m, 2H), 1.63-1.58 (m, 1H), 1.03-0.98 (m, 3H); MS (ESI) m/z 529.3 (M+H).

S16-10-37:

¹H NMR (400 MHz, CD₃OD) δ 6.89, 6.88 (s, 1H total), 4.58, 4.39 (d, J=13.6 Hz, 1H, total), 4.26, 4.03 (d, J=13.6 Hz, 1H, total), 4.03 (s, 1H), 3.12-2.72 (m, 20H), 2.43-2.34 (m, 1H), 2.21-2.18 (m, 1H), 1.78-1.75 (m, 2H), 1.61-1.51 (m, 1H), 0.97-0.90 (m, 3H); MS (ESI) m/z 543 (M+H.

S16-10-38:

¹H NMR (400 MHz, CD₃OD) δ 6.85 (s, 1H), 4.52-4.47 (m, 1H), 4.18-4.15 (m, 1H), 4.07 (s, 1H), 3.16-2.75 (m, 19H), 2.41-2.34 (m, 1H), 2.28-2.25 (m, 1H), 1.74-1.73 (m, 2H), 1.60-1.50 (m, 1H), 1.30-1.25 (m, 3H), 0.96-0.92 (m, 3H); MS (ESI) m/z 557.1 (M+H).

S16-10-39:

¹H NMR (400 MHz, CD₃OD) δ 6.91 (s, 1H), 4.46 (d, J=14.4 Hz, 1H), 4.13 (d, J=14.4 Hz, 1H), 4.08 (s, 1H), 3.08-2.63 (m, 17H), 2.47-2.38 (m, 1H), 2.27-2.22 (m, 1H), 1.75-1.68 (m, 2H), 1.67-1.62 (m, 1H), 1.44-1.42 (m, 2H), 0.98 (t, J=7.2 Hz, 3H); MS (ESI) m/z 543.0 (M+H).

S16-10-40:

¹H NMR (400 MHz, CD₃OD) δ 6.96, 6.95 (s, 1H total), 4.66, 4.46 (d, J=13.6 Hz, 1H total), 4.34, 4.11 (d, J=13.6 Hz, 1H total), 4.11 (s, 1H), 3.20-2.79 (m, 20H), 2.50-2.41 (m, 1H), 2.28-2.24 (m, 1H), 1.81-1.75 (m, 2H), 1.68-1.59 (m, 1H), 1.45-1.38 (m, 2H), 0.97 (m, 3H); MS (ESI) m/z 557.3 (M+H).

S16-10-41:

¹H NMR (400 MHz, CD₃OD) δ 6.99, 6.98 (s, 1H, total), 4.82, 4.67 (d, J=13.6 Hz, 1H, total), 4.43, 4.30 (d, J=13.6 Hz, 1H, total), 4.15 (s, 1H), 3.22-2.88 (m, 19H), 2.58-2.51 (m, 1H), 2.35-2.30 (m, 1H), 1.87-1.79 (m, 1H), 1.75-1.65 (m, 1H), 1.49-1.38 (m, 2H), 1.35-1.145 (m, 2H), 1.03-0.95 (m, 3H), 0.88-0.81 (m, 2H), 0.51-0.40 (m, 2H); MS (ESI) m/z 597.1 (M+H).

S16-10-42:

¹H NMR (400 MHz, CD₃OD) δ 6.96 (s, 1H), 4.42 (d, J=14.4 Hz, 1H), 4.18 (d, J=14.4 Hz, 1H), 4.13 (s, 1H), 3.14-2.84 (m, 17H), 2.51-2.39 (m, 1H), 2.31-2.25 (m, 1H), 1.68-1.64 (m, 4H), 0.99-0.96 (m, 6H); MS (ESI) m/z 557.3 (M+H).

S16-10-43:

¹H NMR (400 MHz, CD₃OD) δ 6.99, 6.97 (s, 1H, total), 4.69, 4.50 (d, J=13.2 Hz, 1H, total), 4.37, 4.14 (d, J=13.2 Hz, 1H, total), 4.12 (s, 1H), 3.09, 2.82 (m, 20H), 2.54-2.45 (m, 1H), 2.30-2.26 (m, 1H), 1.76-1.62 (m, 4H), 1.01-0.97 (m, 6H); MS (ESI) m/z 571.3 (M+H).

S16-10-44:

¹H NMR (400 MHz, CD₃OD) δ 6.97, 6.96 (s, 1H, total), 4.60 (d, J=13.2 Hz, 1H), 4.24 (d, J=13.2 Hz, 1H), 4.12 (s, 1H), 3.23-3.15 (m, 4H), 3.13-2.84 (m, 15H), 2.56-2.41 (m, 1H), 2.30-2.26 (m, 1H), 1.68-1.64 (m, 4H), 1.39-1.34 (m, 3H), 0.97 (d, J=6.4 Hz, 6H); MS (ESI) m/z 585.1 (M+H).

S16-10-45:

¹H NMR (400 MHz, CD₃OD) δ 6.96 (s, 1H), 4.62-4.56 (m, 1H), 4.25-4.22 (m, 1H), 4.12-4.11 (m, 1H), 3.26-2.83 (m, 19H), 2.55-2.41 (m, 1H), 2.29-2.26 (m, 1H), 1.76-1.63 (m, 4H), 1.45-1.37 (m, 7H), 1.00-0.95 (m, 4H); MS (ESI) m/z 611.1 (M+H).

S16-10-46:

¹H NMR (400 MHz, CD₃OD) δ 6.85 (s, 1H), 4.28 (d, J=14.8 Hz, 1H), 4.05 (d, J=14.4 Hz, 1H), 4.03 (s, 1H), 3.05-2.72 (m, 17H), 2.38-2.31 (m, 1H), 2.20-2.17 (m, 1H), 1.67-1.53 (m, 8H), 1.31-1.06 (m, 4H), 0.95-0.85 (m, 2H); MS (ESI) m/z 597.1 (M+H).

S16-10-47:

¹H NMR (400 MHz, CD₃OD) δ 6.85, 6.85 (s, 1H, total), 4.48 (d, J=13.6 Hz, 1H), 4.12 (d, J=13.6 Hz, 1H), 4.01 (s, 1H), 3.25-2.70 (m, 19H), 2.44-2.30 (m, 1H), 2.18-2.13 (m, 1H), 1.68-1.52 (m, 6H), 1.79-1.11 (m, 8H), 0.93-0.85 (m, 3H); MS (ESI) m/z 625.1 (M+H).

S16-10-48:

¹H NMR (400 MHz, CD₃OD) δ 7.39-7.27 (m, 5H), 6.91 (s, 1H), 4.40 (d, J=14.8 Hz, 1H), 4.18 (d, J=14.6 Hz, 1H), 4.11 (s, 1H), 3.42-3.38 (m, 2H), 3.15-2.97 (m, 9H), 2.88-2.58 (m, 6H), 2.44-2.36 (m, 1H), 2.27-2.24 (m, 1H), 1.64-1.58 (m, 1H); MS (ESI) m/z 591.1 (M+H).

S16-10-49:

¹H NMR (400 MHz, CD₃OD) δ 6.83 (s, 1H), 4.30 (d, J=14.4 Hz, 1H), 4.07 (d, J=14.4 Hz, 1H), 3.99 (s, 1H), 3.05-2.77 (m, 17H), 2.45-2.31 m, 1H), 2.17-2.18 (m, 1H), 1.59-1.54 (m, 3H), 0.89 (s, 9H); MS (ESI) m/z 571.1 (M+H).

S16-10-50:

¹H NMR (400 MHz, CD₃OD) δ 7.02, 6.99 (s, 1H, total), 4.71, 4.54 (d, J=13.2 Hz, 1H, total), 4.40, 4.17 (d, J=13.2 Hz, 1H, total), 4.14 (s, 1H), 3.11-2.84 (m, 20H), 2.60-2.48 (m, 1H), 2.32-2.29 (m, 1H), 1.77-1.63 (m, 3H), 1.02, 0.99 (s, 9H, total); MS (ESI) m/z 585.1 (M+H).

S16-10-51:

¹H NMR (400 MHz, CD₃OD) δ 6.96, 6.95 (s, 1H, total), 4.62-4.56 (m, 1H), 4.28-4.22 (m, 1H), 4.10 (s, 1H), 3.25-2.81 (m, 19H), 2.53-2.40 (m, 1H), 2.28-2.23 (m, 1H), 1.69-1.62 (m, 3H), 1.40-1.35 (m, 3H), 0.99, 0.97 (s, 9H total); MS (ESI) m/z 599.1 (M+H).

S16-10-52:

¹H NMR (400 MHz, CD₃OD) δ 6.81 (s, 1H), 4.32 (d, J=14.0 Hz, 1H), 4.08 (d, J=14.0 Hz, 1H), 4.03 (s, 1H), 3.68-3.60 (m, 3H), 3.05-2.73 (m, 17H), 2.45-2.33 (m, 1H), 2.21-2.15 (m, 1H), 1.58-1.50 (m, 1H), 1.15-1.10 (m, 6H); MS (ESI) m/z 573.1 (M+H).

S16-10-53:

¹H NMR (400 MHz, CD₃OD) δ 6.93, 6.91 (s, 1H total), 4.66-4.63 (m, 1H), 4.31-4.25 (m, 1H), 4.11 (s, 1H), 3.82-3.70 (m, 3H), 3.55-3.51 (m, 1H), 3.42-3.37 (m, 2H), 3.18-2.84 (m, 16H), 2.50-2.46 (m, 1H), 2.30-2.27 (m, 1H), 1.66-1.63 (m, 1H), 1.43-1.12 (m, 9H); MS (ESI) m/z 601.1 (M+H).

S16-10-54:

¹H NMR (400 MHz, CD₃OD) δ 6.91, 6.89 (s, 1H total), 4.72-4.69 (m, 1H), 4.48-4.39 (m, 1H), 4.11 (s, 1H), 3.89-3.70 (m, 3H), 3.56-3.39 (m, 4H), 3.05-2.83 (m, 16H), 2.53-2.45 (m, 1H), 2.29-2.26 (m, 1H), 1.66-1.63 (m, 1H), 1.27-1.20 (m, 6H); MS (ESI) m/z 586.9 (M+H).

S16-10-55:

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.50-4.46 (m, 1H), 4.29-4.25 (m, 1H), 4.09 (s, 1H), 3.83-3.59 (m, 6H), 3.21-2.79 (m, 17H), 2.46-2.26 (m, 1H), 2.36-2.31 (m, 1H), 2.39-2.06 (m, 4H), 1.68-1.62 (m, 1H); MS (ESI) m/z 584.3 (M+H).

S16-10-56:

¹H NMR (400 MHz, CD₃OD) δ 7.12 (s, 1H), 4.66-4.62 (m, 1H), 4.36-4.34 (m, 1H), 4.13 (s, 1H), 3.81-3.60 (m, 6H), 3.07-2.87 (m, 20H), 2.56-2.41 (m, 1H), 2.32-2.29 (m, 1H), 2.25-2.08 (m, 4H), 1.73-1.58 (m, 1H); MS (ESI) m/z 598.1 (M+H).

S16-10-57:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (s, 1H), 4.67-4.64 (m, 1H), 4.35-4.32 (m, 1H), 4.13 (s, 1H), 3.82-3.60 (m, 6H), 3.14-2.89 (m, 19H), 2.556-2.42 (m, 1H), 2.31-2.29 (m, 1H), 2.22-2.02 (m, 4H), 1.61-1.72 (m, 1H), 1.45-1.38 (m, 3H); MS (ESI) m/z 612.3 (M+H).

S16-10-58:

¹H NMR (400 MHz, CD₃OD) δ 6.94 (s, 1H), 4.67, 4.54 (d, J=13.6 Hz, 1H, total), 4.53, 4.23 (d, J=13.6 Hz, 1H, total), 4.10 (s, 1H), 3.19-2.83 (m, 19H), 2.55-2.42 (m, 1H), 2.29-2.26 (m, 1H), 2.20-2.16 (m, 1H), 1.66-1.64 (m, 1H), 1.41-1.28 (m, 3H), 1.11-1.01 (m, 6H); MS (ESI) m/z 571.1 (M+H).

S16-10-59:

¹H NMR (400 MHz, CD₃OD) δ 7.01, 6.98 (s, 1H, total), 4.81 (s, 1H), 4.48 (s, 1H), 4.14 (s, 1H), 3.25-2.85 (m, 20H), 2.55-2.43 (m, 1H), 2.32-2.29 (m, 1H), 1.69-1.63 (m, 1H), 1.30-1.20 (m, 1H), 0.89-0.81 (m, 2H), 0.54-0.48 (m, 2H); MS (ESI) m/z 555.0 (M+H).

S16-10-60:

¹H NMR (400 MHz, CD₃OD) δ 6.93, 6.92 (s, 1H, total), 4.76, 4.57 (d, J=13.6 Hz, 1H, total), 4.36, 4.21 (d, J=13.6 Hz, 1H, total), 4.08 (s, 1H), 3.20-2.81 (m, 19H), 2.50-2.45 (m, 1H), 2.26-2.23 (m, 1H), 1.64-1.61 (m, 1H), 1.37, 1.31 (t, J=6.8 Hz, 3H, total), 1.29-1.22 (m, 1H), 0.79-0.74 (m, 2H), 0.48-0.38 (m, 2H); MS (ESI) m/z 569.1 (M+H).

S16-10-61:

¹H NMR (400 MHz, CD₃OD) δ 6.90 (s, 1H), 4.33 (d, J=14.0 Hz, 1H), 4.09 (d, J=14.0 Hz, 1H), 4.08 (s, 1H), 3.12-2.94 (m, 11H), 2.87-2.75 (m, 6H), 2.78-2.63 (m, 1H), 2.45-2.38 (m, 1H), 2.25-2.15 (m, 3H), 2.03-1.82 (m, 4H), 1.69-1.53 (m, 1H); MS (ESI) m/555.2 (M+H).

S16-10-62:

¹H NMR (400 MHz, CD₃OD) δ 6.96, 6.94 (s, 1H, total), 4.60, 4.41 (d, J=13.2 Hz, total), 4.35, 4.08 (d, J=13.2 Hz, 1H, total), 4.11 (s, 1H), 3.09-2.80 (m, 20H), 2.52-2.45 (m, 1H), 2.30-2.20 (m, 3H), 2.08-2.02 (m, 1H), 1.98-1.85 (m, 4H), 1.68-1.62 (m, 1H); MS (ESI) m/z 569.1 (M+H).

S16-10-63:

¹H NMR (400 MHz, CD₃OD) δ 6.96 (s, 1H), 4.57-4.55 (m, 1H), 4.25-4.23 (m, 1H), 4.12 (s, 1H), 3.25-2.82 (m, 19H), 2.57-2.42 (m, 1H), 2.27-2.23 (m, 3H), 2.10-2.05 (m, 1H), 2.00-1.91 (m, 4H), 1.72-1.68 (m, 1H), 1.39-1.35 (m, 3H); MS (ESI) m/z 583.1 (M+H).

S16-10-64:

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 1H), 4.46 (d, J=13.6 Hz, 1H), 4.24 (d, J=13.6 Hz, 1H), 4.15 (s, 1H), 3.27-2.87 (m, 17H), 2.56-2.46 (m, 1H), 2.32-2.29 (m, 1H), 1.96-1.94 (m, 6H), 1.70-1.65 (m, 3H), 0.94 (t, J=7.6 Hz, 3H); MS (ESI) m/z 583.1 (M+H).

S16-10-65:

¹H NMR (400 MHz, CD₃OD) δ 6.98, 6.96 (s, 1H, total), 4.68, 4.45 (d, J=13.2 Hz, 1H, total), 4.34, 4.11 (d, J=13.2 Hz, 1H, total), 4.11 (s, 1H), 3.08-2.78 (m, 20H), 2.55-2.42 (m, 1H), 2.29-2.25 (m, 1H), 2.16-2.12 (m, 1H), 2.01-1.92 (m, 5H), 1.92-1.81 (m, 1H), 1.71-1.60 (m, 2H), 0.97 (t, J=7.6 Hz, 3H); MS (ESI) m/z 597.1 (M+H).

S16-10-66:

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 1H), 4.60, 4.47 (d, J=13.2 Hz, 1H, total), 4.35, 4.24 (d, J=13.2 Hz, 1H, total), 4.13 (s, 1H), 3.47-3.41 (m, 1H), 3.20-2.84 (m, 18H), 2.60-2.50 (m, 1H), 2.31-2.29 (m, 1H), 2.11-1.96 (m, 6H), 1.85-1.61 (m, 3H), 1.45-1.36 (m, 3H), 1.03-0.95 (m, 3H); MS (ESI) m/z 611.1 (M+H).

S16-10-67:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (s, 1H), 4.43 (d, J=13.2 Hz, 1H), 4.19 (d, J=13.2 Hz, 1H), 4.14 (s, 1H), 3.01-2.85 (m, 17H), 2.50-2.43 (m, 1H), 2.32-2.25 (m, 2H), 1.99-1.89 (m, 2H), 1.74-1.66 (m, 5H), 1.33-1.30 (m, 2H); MS (ESI) m/z 569.3 (M+H).

S-16-10-68:

¹H NMR (400 MHz, CD₃OD) δ 6.94 (s, 1H), 4.61, 4.58, 4.50, 4.47 (s, 1H, total), 4.26, 4.23, 4.20, 4.17 (s, 1H, total), 4.11 (s, 1H), 3.55-3.45 (m, 2H), 3.10-2.85 (m, 17H), 2.51-2.48 (m, 1H), 2.31-2.28 (m, 1H), 2.05-1.75 (m, 2H), 1.66-1.62 (m, 2H), 1.46-1.30 (m, 6H), 1.12-1.01 (m, 3H); MS (ESI) m/z 597.0 (M+H).

S16-10-69:

¹H NMR (400 MHz, CD₃OD) δ 6.93 (s, 1H), 4.71 (d, J=13.2 Hz, 1H), 4.38 (s, 1H), 4.11 (s, 1H), 3.09-2.79 (m, 20H), 2.52-2.42 (m, 1H), 2.27-2.25 (m, 1H), 1.95-1.92 (m, 1H), 1.81-1.62 (m, 6H), 1.37-1.25 (m, 3H), 1.08-1.03 (m, 2H); MS (ESI) m/z 597.1 (M+H).

S16-10-70:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (s, 1H), 4.71, 4.57 (d, J=13.6 Hz, 1H, total), 4.37, 4.24 (d, J=13.6 Hz, 1H, total), 4.14 (s, 1H), 3.22-2.86 (m, 19H), 2.58-2.52 (m, 1H), 2.32-2.29 (m, 1H), 1.98-1.62 (m, 7H), 1.45-1.33 (m, 5H), 1.33-1.25 (m, 1H), 1.20-1.05 (m, 2H) MS (ESI) m/z 611.1 (M+H).

S16-10-71:

¹H NMR (400 MHz, CD₃OD) δ 6.93 (s, 1H), 4.42-4.39 (m, 1H), 4.19-4.16 (m, 1H), 4.11 (s, 1H), 3.10-2.82 (m, 17H), 2.49-2.42 (m, 1H), 2.28-2.20 (m, 1H), 1.68-1.25 (m, 1H), 1.09 (s, 3H); MS (ESI) m/z 597.3 (M+H).

S16-10-72:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.73, 4.72, 4.58, 4.55 (m, 1H, total), 4.39, 4.36, 4.19, 4.16 (m, 1H, total), 4.10 (s, 1H), 3.28 (s, 2H), 3.13-2.78 (m, 18H), 2.52-2.40 (m, 1H), 2.29-2.26 (m, 1H), 1.70-1.60 (m, 1H), 1.67-1.35 (m, 10H), 1.10 (s, 3H); MS (ESI) m/z 611.4 (M+H).

S16-10-73:

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 1H), 4.93, 4.90, 4.62, 4.59 (s, 1H, total), 4.49, 4.46, 4.32, 4.29 (s, 1H, total), 4.15 (s, 1H), 3.17-2.85 (m, 19H), 2.55 (m, 1H), 2.36-2.30 (m, 1H), 1.73-1.65 (m, 1H), 1.55-1.35 (m, 13H), 1.17-1.15 (m, 3H); MS (ESI) m/z 625.1 (M+H).

S16-10-74:

¹H NMR (400 MHz, CD₃OD) δ 6.96 (s, 1H), 4.40 (d, J=14.0 Hz, 1H), 4.17 (d, J=14.0 Hz, 1H), 4.14 (s, 1H), 3.06-2.84 (m, 17H), 2.55-2.42 (m, 1H), 2.31-2.20 (m, 1H), 2.01-1.96 (m, 1H), 1.83-1.57 (m, 11H), 1.38-1.28 (m, 2H); MS (ESI) m/z 597.1 (M+H).

S16-10-75:

¹H NMR (400 MHz, CD₃OD) δ 6.93 (s, 1H), 4.71, 4.68, 4.43, 4.40 (m, 1H, total), 4.37, 4.68, 4.06, 4.03, (m, 1H, total), 4.11 (s, 1H), 3.11-2.78 (m, 20H), 2.51-2.40 (m, 1H), 2.28-2.25 (m, 1H), 2.10 (s, 1H), 1.77-1.54 (m, 11H), 1.31-1.26 (m, 2H); MS (ESI) m/z 611.1 (M+H).

S16-10-76:

¹H NMR (400 MHz, CD₃OD) δ 6.94 (s, 1H), 4.68, 4.65, 4.51, 4.47 (s, 1H, total), 4.33, 4.30, 4.17, 4.13 (s, 1H, total), 4.10 (s, 1H), 3.16-2.82 (m, 19H), 2.53-2.41 (m, 1H), 2.38-2.33 (m, 1H), 2.13-2.10 (m, 1H), 1.77-1.58 (m, 11H), 1.39-1.30 (m, 2H), 1.28-1.21 (m, 3H); MS (ESI) m/z 625.3 (M+H).

S16-10-77:

¹H NMR (400 MHz, CD₃OD) δ 6.97, 6.93 (s, 1H, total), 4.65, 4.62, 4.36, 4.33 (s, 1H, total), 4.39 (s, 1H), 4.10 (s, 1H), 3.28-3.31 (m, 4H), 3.13-2.80 (m, 15H), 2.53-2.41 (m, 1H), 2.29-2.26 (m, 1H), 1.64-1.62 (m, 1H), 1.41-1.34 (m, 3H), 2.24 (t, J=6.8 Hz, 2H), 1.09-1.04 (m, 6H); MS (ESI) m/z 585.1 (M+H).

S16-10-78:

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.79, 4.76, 4.65, 4.62 (s, 1H, total), 4.47, 4.43, 4.41, 4.37 (s, 1H, total), 4.13 (s, 1H), 3.53-3.51 (m, 1H), 3.19-2.89 (m, 18H), 2.53-2.41 (m, 1H), 2.32-2.29 (m, 1H), 1.68-1.63 (m, 1H), 1.31-1.25 (m, 1H), 1.15 (s, 9H), 0.86-0.80 (m, 2H), 0.50-0.39 (m, 2H); MS (ESI) m/z 611.1 (M+H).

S16-10-79:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (s, 1H), 4.45-4.42 (m, 1H), 4.31-4.27 (m, 1H), 4.12 (s, 1H), 3.12-2.82 (m, 16H), 2.49-2.42 (m, 1H), 2.31-2.28 (m, 1H), 1.68-1.62 (m, 1H), 1.40 (d, J=7.2 Hz, 3H), 1.08 (s, 9H); MS (ESI) m/z 571.3 (M+H).

S16-10-80:

¹H NMR (400 MHz, CD₃OD) δ 7.07, 6.97 (s, 1H, total), 4.66 (d, J=11.6 Hz, 1H), 4.33 (d, J=13.2 Hz, 1H), 4.15 (s, 1H), 3.53-3.50 (m, 1H), 3.23-2.82 (m, 18H), 2.55-2.46 (m, 1H), 2.33-2.30 (m, 1H), 1.71-1.62 (m, 1H), 1.52, 1.43 (s, 3H, total), 1.24 (s, 3H), 1.10 (s, 6H), MS (ESI) m/z 585.1 (M+H).

S16-10-81:

¹H NMR (400 MHz, CD₃OD) δ 7.01, 6.94 (s, 1H, total), 4.60 (d, J=13.6 Hz, 1H), 4.19 (d, J=13.6 Hz, 1H), 4.11 (s, 1H), 3.61-3.59 (m, 1H), 3.19-2.89 (m, 17H), 2.55-3.42 (m, 1H), 2.29-2.26 (m, 1H), 1.64-1.59 (m, 1H), 1.49-1.41 (m, 4H), 1.30 (t, J=6.8 Hz, 2H), 1.28, 0.95 (s, 9H, total); MS (ESI) m/z 599.2 (M+H).

S16-10-82:

¹H NMR (400 MHz, CD₃OD) δ 7.01, 6.94 (s, 1H, total), 4.63, 4.60, 4.17, 4.14 (s, 1H, total), 4.12 (s, 1H), 3.88-3.86 (m, 1H), 3.19-2.90 (m, 18H), 2.55-2.42 (m, 1H), 2.31-2.28 (m, 1H), 1.68-1.63 (m, 1H), 1.55-1.52 (m, 2H), 1.45-1.41 (m, 1H), 1.22 (s, 6H), 1.06-1.02 (m, 1H), 0.96 (s, 3H), 0.82-0.75 (m, 1H), 0.71-0.63 (m, 1H), 0.42-0.36 (m, 1H), 0.25-0.20 (m, 1H); MS (ESI) m/z 625.3 (M+H).

S16-10-83:

¹H NMR (400 MHz, CD₃OD) δ 6.84 (s, 1H), 4.46 (d, J=14.0 Hz, 1H), 4.14 (d, J=14.0 Hz, 1H), 4.05 (s, 1H), 3.96-3.92 (m, 1H), 3.82-3.77 (m, 1H), 3.06-2.84 (m, 16H), 2.43-2.37 (m, 1H), 2.24-2.20 (m, 1H), 1.62-1.53 (m, 1H), 1.06 (s, 9H); MS (ESI) m/z 587.1 (M+H)

S16-10-84

¹H NMR (400 MHz, CD₃OD) δ 6.94 (s, 1H), 4.79-4.76 (m, 1H), 4.58-4.52 (m, 1H), 4.13 (s, 1H), 4.08-4.05 (m, 1H), 3.99-3.94 (m, 1H), 3.49-3.47 (m, 1H), 3.12-2.80 (m, 18H), 2.52-2.48 (m, 1H), 2.30-2.28 (m, 1H), 1.68-1.63 (m, 1H), 1.28-1.10 (m, 9H); MS (ESI) m/z 601.1 (M+H).

S16-10-85:

¹H NMR (400 MHz, CD₃OD) δ 7.01, 6.92 (s, 1H total), 4.56-4.54 (m, 1H), 4.13 (s, 3H), 3.60-3.48 (m, 2H), 3.21-2.91 (m, 17H), 2.58-2.51 (m, 1H), 2.31-2.28 (m, 1H), 1.71-1.61 (m, 1H), 1.29, 1.25 (s, 9H total), 1.09-1.07 (m, 3H); MS (ESI) m/z 615.4 (M+H)

S16-10-86:

¹H NMR (400 MHz, CD₃OD) δ 6.93 (s, 1H), 4.32 (d, J=14.0 Hz, 1H), 4.11 (d, J=14.0 Hz, 1H), 4.07 (s, 1H), 3.44-3.41 (m, 1H), 3.02-2.77 (m, 15H), 2.48-2.37 (m, 1H), 2.25-2.22 (m, 1H), 1.66-1.56 (m, 1H), 1.38-1.36 (m, 6H); MS (ESI) m/z 529.0 (M+H).

S16-10-87:

¹H NMR (400 MHz, CD₃OD) δ 6.95 (s, 1H), 4.62-4.56 (m, 1H), 4.28-4.25 (m, 1H), 4.10 (s, 1H), 3.08-2.82 (m, 18H), 2.53-2.49 (m, 1H), 2.28-2.23 (m, 1H), 1.68-1.62 (m, 1H), 1.48-1.43 (m, 2H), 1.42-1.31 (m, 7H); MS (ESI) m/z 557.1 (M+H).

S16-10-88:

¹H NMR (400 MHz, CD₃OD) δ 6.89 (s, 1H), 4.33-4.29 (m, 1H), 4.12-4.07 (m, 1H), 4.04 (s, 1H), 3.05-2.74 (m, 16H), 2.42-2.31 (m, 1H), 2.21-2.18 (m, 1H), 1.89-1.81 (m, 1H), 1.59-1.53 (m, 2H), 1.32 (d, J=6.4 Hz, 3H), 0.97 (t, J=6.4 Hz, 3H); MS (ESI) m/z 543.1 (M+H).

S16-10-89:

¹H NMR (400 MHz, CD₃OD) δ 6.97, 6.96 (s, 1H, total), 4.68-4.63 (m, 1H), 4.42-4.38 (m, 1H), 4.11 (s, 1H), 3.48-3.43 (m, 1H), 3.10-2.80 (m, 18H), 2.51-2.45 (m, 1H), 2.31-2.28 (m, 1H), 1.91-1.85 (m, 1H), 1.71-1.60 (m, 2H), 1.43-1.43 (m, 3H), 1.09-1.01 (m, 3H) MS (ESI) m/z 557.1 (M+H).

S16-10-90:

¹H NMR (400 MHz, CD₃OD) δ 6.94 (s, 1H), 4.68, 4.64, 4.54, 4.51 (s, 1H, total), 4.34, 4.31, 4.23, 4.20 (s, 1H, total), 4.10 (s, 1H), 3.18-2.83 (m, 18H), 2.55-2.42 (m, 1H), 2.37-2.34 (m, 1H), 2.29-2.26 (m, 1H), 2.02-1.95 (m, 2H), 1.67 (s, 3H), 1.39-1.28 (m, 6H); MS (ESI) m/z 571.1 (M+H).

S16-10-91:

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.44 (d, J=13.6 Hz, 1H), 4.20 (d, J=13.2 Hz, 1H), 4.14 (s, 1H), 3.21-2.88 (m, 16H), 2.54-2.42 (m, 1H), 2.33-2.29 (m, 1H), 2.21-2.13 (m, 1H), 1.68-1.63 (m, 1H), 1.39-1.31 (m, 3H), 1.10-1.03 (m, 6H), MS (ESI) m/z 557.1 (M+H).

S16-10-92:

¹H NMR (400 MHz, CD₃OD) δ 7.01, 6.99 (s, 1H, total), 4.70, 4.67, 4.56, 4.53 (s, 1H, total), 4.44, 4.41, 4.02, 3.99 (s, 1H, total), 4.17 (s, 1H), 3.19-2.86 (m, 19H), 2.53-2.48 (m, 1H), 2.35-2.27 (m, 1H), 2.16-2.12 (m, 1H), 1.72-1.62 (m, 1H), 1.47, 1.45, 1.41, 1.40 (s, 3H, total), 1.22-1.06 (m, 6H); MS (ESI) m/z 571.3 (M+H).

S16-10-93:

¹H NMR (400 MHz, CD₃OD) δ 7.00, 6.98 (s, 1H, total), 4.69 (t, J=28.8, 14.0 Hz, 1H), 4.34, 4.30, 4.14, 4.10 (s, 1H, total), 4.16 (s, 1H), 3.52-3.43 (m, 1H), 3.23-2.92 (m, 17H), 2.60-2.50 (m, 1H), 2.34-2.31 (m, 1H), 2.28-2.13 (m, 1H), 1.75-1.62 (m, 1H), 1.51, 1.33 (t, J=7.2 Hz, 3H, total), 1.43-1.38 (m, 3H), 1.20, 1.14, 1.05 (d, J=6.4 Hz, 6H, total); MS (ESI) m/z 585.1 (M+H).

S16-10-94:

¹H NMR (400 MHz, CD₃OD) δ 7.03, 7.01 (s, 1H, total), 5.49, 5.45, 4.74, 4.70 (s, 1H, total), 4.34, 4.31, 4.17, 4.13 (s, 1H, total), 4.17 (s, 1H), 3.21-2.93 (m, 18H), 2.60-2.49 (m, 1H), 2.37-2.33 (m, 1H), 2.28-2.16 (m, 1H, total), 1.73-1.68 (m, 1H), 1.49, 1.44 (d, J=6.8 Hz, 3H, total), 1.28, 1.22 (d, J=6.4 Hz, 3H, total), 1.09-1.05 (m, 3H), 0.98-0.92 (m, 1H), 0.88-0.83 (m, 1H), 0.77-0.54 (m, 1H), 0.55-0.48 (m, 1H), 0.28-0.23 (m, 1H); MS (ESI) m/z 611.3 (M+H).

S16-10-95:

¹H NMR (400 MHz, CD₃OD) δ 7.02 (s, 1H), 4.43 (d, J=14.0 Hz, 1H), 4.23 (d, J=13.6 Hz, 1H), 4.15 (s, 1H), 3.14-2.87 (m, 16H), 2.48-2.40 (m, 1H), 2.34-2.29 (m, 1H), 2.18-2.16 (m, 1H), 1.73-1.53 (m, 1H), 1.35 (d, J=6.8 Hz, 3H), 1.07 (d, J=6.8 Hz, 3H), 1.05 (d, J=6.8 Hz, 3H); MS (ESI) m/z 557.3 (M+H).

S16-10-96:

¹H NMR (400 MHz, CD₃OD) δ 6.88 (s, 1H), 4.60-4.57 (m, 1H), 4.20-4.16 (m, 1H), 4.02 (s, 1H), 2.97-2.58 (m, 19H), 2.52-2.36 (m, 1H), 2.20-2.18 (m, 1H), 2.05-1.90 (m, 1H), 1.60-1.48 (m, 1H), 1.33 (d, J=6.4 Hz, 3H), 1.07 (d, J=6.4 Hz, 3H), 1.02 (d, J=6.4 Hz, 3H); MS (ESI) m/z 571.1 (M+H)

S16-10-97:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (s, 1H), 4.70, 4.67, 4.28, 4.25 (s, 1H, total), 4.85, 4.46 (s, 1H, total), 4.13 (s, 1H), 3.16-2.83 (m, 18H), 2.54-2.45 (m, 1H), 2.32-2.23 (m, 1H), 2.02-1.95 (m, 1H), 1.67-1.64 (m, 1H), 1.45-1.33 (m, 3H), 1.28-1.26 (m, 1H), 1.18-1.13 (m, 9H); MS (ESI) m/z 585.3 (M+H).

S16-10-98:

¹H NMR (400 MHz, CD₃OD) δ 6.99, 6.93 (s, 1H, total), 4.63, 4.61, 4.59, 4.58 (s, 1H, total), 4.50, 4.47, 4.43, 4.40 (s, 1H, total), 4.11 (s, 1H), 3.14-2.85 (m, 18H), 2.56-2.45 (m, 1H), 2.32-2.25 (m, 1H), 2.15-2.08 (m, 1H), 1.69-1.60 (m, 1H), 1.41, 1.34 (d, J=8.0 Hz, 3H, total), 1.15 (d, J=6.8 Hz, 3H), 1.07 (d, J=7.2 Hz, 3H), 0.83-0.79 (m, 2H), 0.65-0.58 (m, 1H), 0.48-0.43 (m, 1H), 0.38-0.21 (m, 1H); MS (ESI) m/z 611.2 (M+H).

S16-10-99:

¹H NMR (400 MHz, CD₃OD) δ 7.05, 7.03 (s, 1H, total), 4.73 (s, 1H), 4.45 (s, 1H), 4.14 (s, 1H), 3.10-2.85 (m, 19H), 2.50-2.40 (m, 1H), 2.31-2.28 (m, 1H), 1.72-1.63 (m, 1H), 1.00-0.95 (m, 4H); MS (ESI) m/z 541.1 (M+H).

S16-10-100:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (s, 1H), 4.81, 4.77 (s, 1H, total), 4.52, 4.48 (s, 1H, total), 4.18 (s, 1H), 3.16-2.90 (m, 18H), 2.57-2.43 (m, 1H), 2.36-2.33 (m, 1H), 1.76-1.73 (m, 1H), 1.53 (t, J=7.2 Hz, 3H), 1.20-1.03 (m, 3H), 0.95-0.82 (m, 1H); MS (ESI) m/z 555.1 (M+H).

S16-10-101:

¹H NMR (400 MHz, CD₃OD) δ 6.95 (s, 1H), 4.48 (d, J=13.6 Hz, 1H), 4.25 (d, J=12.8 Hz, 1H), 4.12 (s, 1H), 3.08-2.80 (m, 15H), 2.48-2.38 (m, 1H), 2.28-2.25 (m, 1H), 1.68-1.63 (m, 1H), 1.58 (s, 3H), 1.15 (s, 2H), 0.88 (s, 2H); MS (ESI) m/z 541.1 (M+H).

S16-10-102:

¹H NMR (400 MHz, CD₃OD) δ 6.95 (s, 1H), 4.46 (d, J=14 Hz, 1H), 4.14 (s, 1H), 4.11 (d, J=14 Hz, 1H), 3.88-3.84 (m, 1H), 3.07-2.45 (m, 15H), 2.45-2.27 (m, 6H), 1.99-1.92 (m, 2H), 1.71-1.62 (m, 1H); MS (ESI) m/z 541.1 (M+H).

S16-10-103:

¹H NMR (400 MHz, CD₃OD) δ 7.08, 7.04 (s, 1H, total), 4.52, 4.48, 4.32, 4.29 (s, 1H, total), 4.11 (s, 1H), 4.24, 4.21, 4.02, 3.99 (s, 1H, total), 3.90-3.86 (m, 1H), 3.10-2.81 (m, 15H), 2.65 (s, 3H), 2.48-2.37 (m, 1H), 2.35-2.24 (m, 5H), 1.89-1.81 (m, 2H), 1.68-1.62 (m, 1H); MS (ESI) m/z 555.1 (M+H).

S16-10-104:

¹H NMR (400 MHz, CD₃OD) δ 7.01, 6.97 (s, 1H, total), 4.52, 4.49, 4.45, 4.41 (s, 1H, total), 4.12 (s, 1H), 4.20, 4.17, 4.12, 4.09 (s, 1H, total), 4.00-3.97 (m, 1H), 3.18-2.84 (m, 17H), 2.49-2.38 (m, 1H), 2.39-2.20 (m, 5H), 1.88-1.82 (m, 2H), 1.68-1.62 (m, 1H), 1.35-1.28 (m, 3H); MS (ESI) m/z 569.1 (M+H).

S16-10-105:

¹H NMR (400 MHz, CD₃OD) δ 7.01, 6.99 (s, 1H, total), 4.68, 4.65, 4.52, 4.49 (s, 1H, total), 4.35, 4.31, 4.11, 4.08 (s, 1H, total), 4.11 (s, 1H), 4.19-4.16 (m, 1H), 3.10-2.85 (m, 17H), 2.50-2.40 (m, 1H), 2.38-2.33 (m, 2H), 2.30-2.15 (m, 3H), 1.90-1.78 (m, 2H), 1.68-1.62 (m, 1H), 1.15-1.08 (m, 1H), 0.76-0.74 (m, 2H), 0.48-0.42 (m, 2H); MS (ESI) m/z 595.1 (M+H).

S16-10-106:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (s, 1H), 4.22, 4.20 (s, 1H, total), 4.13 (s, 1H), 4.06, 4.04 (s, 1H, total), 3.03-2.83 (m, 15H), 2.50-2.40 (m, 3H), 2.23-2.29 (m, 1H), 2.16-2.14 (m, 2H), 2.04-1.99 (m, 2H), 1.72-1.65 (m, 1H), 1.65 (s, 3H); MS (ESI) m/z 555.1 (M+H).

S16-10-107:

¹H NMR (400 MHz, CD₃OD) δ 7.01, 6.97 (s, 1H, total), 4.43, 4.40, 4.22, 4.19 (s, 1H, total), 4.17, 4.14, 3.95, 3.92 (s, 1H, total), 4.12 (s, 1H), 3.08-2.79 (m, 15H), 2.67, 2.59 (s, 3H, total), 2.47-2.41 (m, 3H), 2.28-2.20 (m, 2H), 2.11-2.04 (m, 1H), 1.92-1.88 (m, 2H), 1.64-1.60 (m, 1H), 1.60 (s, 3H); MS (ESI) m/z 569.3 (M+H).

S16-10-108:

¹H NMR (400 MHz, CD₃OD) δ 7.05, 7.04, 7.01, 7.00 (s, 1H, total), 4.49-4.38 (m, 1H), 4.17-4.12 (m, 2H), 3.22-2.85 (m, 17H), 2.65-2.21 (m, 4H), 2.20-2.05 (m, 2H), 1.98-1.89 (m, 2H), 1.68-1.61 (m, 4H), 1.23-1.15 (m, 3H); MS (ESI) m/z 583.2 (M+H).

S16-10-109:

¹H NMR (400 MHz, CD₃OD) δ 7.07, 7.05 (s, 1H, total), 4.57, 4.54, 4.48, 4.45 (s, 1H, total), 4.23, 4.20, 4.18, 4.15 (s, 1H, total), 4.13 (s, 1H), 3.14-2.93 (m, 17H), 2.54-2.51 (m, 2H), 2.45-2.41 (m, 1H), 2.35-2.18 (m, 3H), 1.99-1.89 (m, 3H), 1.68-1.65 (m, 4H), 0.95-0.60 (m, 2H), 0.40-0.20 (m, 2H); MS (ESI) m/z 609.1 (M+H).

S16-10-110:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (s, 1H), 4.38 (d, J=14.0 Hz, 1H), 4.17 (d, J=13.6 Hz, 1H), 4.12 (s, 1H), 3.69-3.62 (m, 1H), 3.06-2.82 (m, 15H), 2.49-2.38 (m, 1H), 2.29-2.22 (m, 1H), 2.18-2.04 (m, 2H), 1.85-1.82 (m, 2H), 1.78-1.61 (m, 5H); MS (ESI) m/z 555.3 (M+H).

S16-10-111:

¹H NMR (400 MHz, CD₃OD) δ 7.03 (d, J=6.8 Hz, 1H), 4.68 (d, J=13.2 Hz, 1H), 4.51-4.39 (m, 1H), 4.16 (s, 1H), 3.84-3.78 (m, 1H), 3.09-2.86 (m, 19H), 2.55-2.48 (m, 1H), 2.34-2.30 (m, 2H), 1.91 (s, 5H), 1.77-1.68 (m, 3H); MS (ESI) m/z 569.1 (M+H).

S16-10-112:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (m, J=7.6 Hz, 1H), 4.71-4.59 (m, 1H), 4.34-4.25 (m, 1H), 4.15 (s, 1H), 3.27-2.87 (m, 18H), 2.56-2.48 (m, 1H), 2.34-2.20 (m, 2H), 1.89 (s, 5H), 1.75-1.67 (m, 3H), 1.43-1.37 (m, 3H); MS (ESI) m/z 583.1 (M+H).

S16-10-113:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (s, 1H), 4.40 (d, J=14 Hz, 1H), 4.19 (d, J=13.6 Hz, 1H), 4.11-4.05 (m, 3H), 4.01-3.98 (m, 1H), 3.75-3.71 (m, 1H), 3.09-2.80 (m, 16H), 2.49-2.41 (m, 2H), 2.29-2.26 (m, 1H), 2.15-2.09 (m, 1H), 1.68-1.63 (m, 1H); MS (ESI) m/z 557.1 (M+H).

S16-10-114:

¹H NMR (400 MHz, CD₃OD) δ 7.48 (s, 1H), 4.65-4.62, 4.49-4.46 (m, 1H, total), 4.33-4.28 (m, 2H), 4.18-4.10 (m, 3H), 3.75-3.68 (m, 1H), 3.09-2.82 (m, 19H), 2.50-2.41 (m, 2H), 2.35-2.25 (m, 2H), 1.68-1.63 (m, 1H); MS (ESI) m/z 571.1 (M+H).

S16-10-115:

¹H NMR (400 MHz, CD₃OD) δ 6.96 (s, 1H), 4.71-4.55 (m, 1H), 4.28-4.23 (m, 2H), 4.19-4.13 (m, 2H), 4.11 (s, 1H), 3.68-3.63 (m, 1H), 3.09-2.81 (m, 18H), 2.53-2.14 (m, 4H), 1.68-1.59 (m, 1H), 1.41-1.33 (m, 3H); MS (ESI) m/z 585.1 (M+H).

S16-10-116:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (s, 1H), 4.45, 4.42 (s, 1H, total), 4.22, 4.18 (s, 1H, total), 4.13 (s, 1H), 3.93-3.88 (m, 2H), 3.80-3.73 (m, 1H), 3.59-3.55 (m, 1H), 3.17-2.83 (m, 17H), 2.69-2.66 (m, 1H), 2.51-2.40 (m, 1H), 2.30-2.17 (m, 2H), 1.75-1.60 (m, 2H); MS (ESI) m/z 571.1 (M+H).

S16-10-117:

¹H NMR (400 MHz, CD₃OD) δ 7.25-7.16 (m, 4H), 6.90 (s, 1H), 4.37 (d, J=14 Hz, 1H), 4.13-4.04 (m, 2H), 4.04 (s, 1H), 3.61-3.39 (m, 3H), 3.17-2.58 (m, 16H), 2.39-2.31 (m, 1H), 2.21-2.18 (m, 1H), 1.61-1.52 (m, 1H); MS (ESI) m/z 603.0 (M+H).

S16-10-118:

¹H NMR (400 MHz, CD₃OD) δ 7.25-7.18 (m, 4H), 6.95, 6.93 (s, 1H, total), 4.58, 4.54, 4.45, 4.42 (s, 1H, total), 4.36, 4.33, 4.06, 4.03 (s, 1H, total), 4.28-4.23 (m, 2H), 4.04 (s, 1H), 3.52-3.27 (m, 6H), 2.98-2.71 (m, 15H), 2.45-2.38 (m, 1H), 2.21-2.18 (m, 1H), 1.63-1.54 (m, 1H); MS (ESI) m/z 617.1 (M+H)

S16-10-119:

¹H NMR (400 MHz, CD₃OD) δ 6.89 (s, 1H), 4.22 (d, J=14.2 Hz, 1H), 4.03 (d, J=14.4 Hz, 1H), 4.01 (s, 1H), 2.93-2.70 (m, 15H), 2.48-2.38 (m, 1H), 2.18-2.14 (m, 1H), 1.85-1.69 (m, 8H), 1.56-1.47 (m, 1H), 1.38 (s, 3H); MS (ESI) m/z 569.0 (M+H).

S16-10-120:

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 1H), 4.43-4.39 (m, 1H), 4.22-4.19 (m, 1H), 4.14 (s, 1H), 3.23-2.91 (m, 9H), 2.96-2.83 (m, 6H), 2.52-2.42 (m, 1H), 2.31-2.28 (m, 1H), 2.25-2.15 (m, 2H), 1.93-1.87 (m, 2H), 1.75-1.61 (m, 2H), 1.49-1.25 (m, 6H); MS (ESI) m/z 569.3 (M+H).

S16-10-121:

¹H NMR (400 MHz, CD₃OD) δ 6.97, 6.96 (s, 1H total), 4.69, 4.66, 4.41, 4.39 (s, 1H total), 4.38, 4.37, 4.04, 4.01 (s, 1H total), 4.11 (s, 1H), 3.21-2.73 (m, 19H), 2.54-2.47 (m, 1H), 2.30-2.26 (m, 1H), 2.13-2.10 (m, 2H), 2.00-1.92 (m, 1H), 1.76-1.57 (m, 4H), 1.44-1.20 (m, 4H); MS (ESI) m/z 583.4 (M+H).

S16-10-122:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (s, 1H), 4.74, 4.71, 4.57, 4.54 (s, 1H, total), 4.40, 4.37, 4.19, 4.15 (s, 1H, total), 4.15 (s, 1H), 3.50-3.47 (m, 1H), 3.26-2.86 (m, 17H), 2.54-2.45 (m, 1H), 2.37-2.32 (m, 1H), 2.18-1.95 (m, 4H), 1.85-1.61 (m, 4H), 1.51-1.28 (m, 6H); MS (ESI) m/z 597.1 (M+H).

S16-10-123:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.37, 4.34 (s, 1H, total), 4.14, 4.11 (s, 1H, total), 4.11 (s, 1H), 3.09-2.82 (m, 15H), 2.48-2.38 (m, 1H), 2.30-2.25 (m, 1H), 1.96-1.84 (m, 3H), 1.75-1.54 (m, 8H), 1.47 (s, 3H); MS (ESI) m/z 583.1 (M+H).

S16-10-124:

¹H NMR (400 MHz, CD₃OD) δ 7.08, 7.04 (s, 1H, total), 4.79, 4.76, 4.54, 4.51 (s, 1H, total), 4.48, 4.45, 4.24, 4.21 (s, 1H, total), 4.60, 4.16 (s, 1H, total), 3.27-2.86 (m, 17H), 2.54-2.47 (m, 1H), 2.33-2.30 (m, 1H), 1.73-1.66 (m, 1H), 1.60-1.58 (m, 9H), 1.22-1.20 (m, 3H); MS (ESI) m/z 571.1 (M+H).

S16-10-125:

¹H NMR (400 MHz, CD₃OD) δ 7.10, 7.08 (s, 1H, total), 4.85, 4.81, 4.26, 4.23 (s, 1H, total), 4.54 (s, 1H), 4.18 (s, 1H), 3.59-3.54 (m, 1H), 3.29-3.27 (m, 1H), 3.18-2.89 (m, 15H), 2.54-2.42 (m, 1H), 2.36-2.34 (m, 1H), 2.08-2.04 (m, 1H), 1.98-1.92 (m, 1H), 1.70-1.61 (m, 1H), 1.58-1.56 (m, 6H), 1.24-1.19 (m, 6H); MS (ESI) m/z 585.4 (M+H).

S16-10-126:

¹H NMR (400 MHz, CD₃OD) δ 7.02 (s, 1H), 4.36 (d, J=13.2 Hz, 1H), 4.15-4.11 (d, J=13.2 Hz, 1H), 4.11 (s, 1H), 3.06-2.84 (m, 16H), 2.49-4.41 (m, 1H), 2.35-2.27 (m, 1H), 1.82 (d, J=7.2 Hz, 2H), 1.68-1.63 (m, 1H), 1.57 (s, 6H), 1.12 (s, 9H); MS (ESI) m/z 599.6 (M+H).

S16-10-127:

¹H NMR (400 MHz, CD₃OD) δ 7.00, 6.98 (s, 1H, total), 4.87, 4.83, 4.62, 4.59 (s, 1H, total), 4.16 (s, 1H), 4.24, 4.21, 3.99, 3.96 (s, 1H, total), 3.14-2.71 (m, 18H), 2.52-2.41 (m, 1H), 2.33-2.30 (m, 1H), 2.01 (s, 1H), 1.90-1.81 (m, 1H), 1.70 (s, 3H), 1.68-1.66 (m, 1H), 1.65 (s, 3H), 1.17 (s, 9H); MS (ESI) m/z 613.1

S16-10-128:

¹H NMR (400 MHz, CD₃OD) δ 7.04, 7.01 (s, 1H, total), 4.78, 4.74 (s, 1H, total), 4.45 (s, 1H), 4.15, 4.10 (s, 1H, total), 3.58-3.51 (m, 1H), 3.19-2.87 (m, 16H), 2.53-2.46 (m, 1H), 2.28-2.21 (m, 1H), 1.94-1.90 (m, 1H), 1.88-1.83 (m, 1H), 1.69-1.63 (m, 7H), 1.12 (s, 12H), MS (ESI) m/z 627.1 (M+H).

S16-10-129:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.43, 4.39 (s, 1H, total), 4.34, 4.31 (s, 1H, total), 4.14 (s, 1H), 3.13-2.77 (m, 16H), 2.49-2.40 (m, 1H), 2.31-2.28 (m, 1H), 1.67-1.65 (m, 1H), 1.51, 1.49 (s, 3H, total), 1.13-1.05 (m, 1H), 0.82-0.76 (m, 2H), 0.61-0.55 (m, 1H), 0.41-0.37 (m, 1H), MS (ESI) m/z 555.0 (M+H).

S16-10-130:

¹H NMR (400 MHz, CD₃OD) δ 6.87, 6.85 (s, 1H, total), 4.70, 4.67, 4.41, 4.37 (s, 1H, total), 4.27, 4.23, 3.97, 3.94 (s, 1H, total), 4.01 (s, 1H), 2.94-2.67 (m, 19H), 2.41-2.32 (m, 1H), 2.19-2.13 (m, 1H), 1.58-1.53 (m, 1H), 1.42-1.37 (m, 3H), 1.21-1.09 (m, 1H), 0.75-0.68 (m, 2H), 0.63-0.60 (m, 1H), 0.38-0.33 (m, 1H); MS (ESI) m/z 569.3 (M+H).

S16-10-131:

¹H NMR (400 MHz, CD₃OD) δ 6.87, 6.84 (s, 1H, total), 4.75, 4.71, 4.48, 4.44 (s, 1H, total), 4.31, 4.28, 4.04, 4.01 (s, 1H, total), 4.02 (s, 1H), 2.94-2.75 (m, 18H), 2.48-2.38 (m, 1H), 2.20-2.15 (m, 1H), 1.58-1.53 (m, 1H), 1.37 (d, J=6.8 Hz, 3H), 1.35-1.27 (m, 1H), 1.13 (t, J=6.4 Hz, 3H), 0.79-0.70 (m, 2H), 0.51-0.40 (m, 2H); MS (ESI) m/z 583.2 (M+H).

S16-10-132:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (s, 1H), 4.64 (d, J=13.2 Hz, 1H), 4.37 (d, J=13.2 Hz, 1H), 4.09 (s, 1H), 3.12-2.81 (m, 15H), 2.46-2.42 (m, 1H), 2.28-2.24 (m, 1H), 1.67-1.55 (m, 2H), 1.05-1.04 (m, 2H), 0.84-0.82 (m, 2H), 0.75-0.73 (m, 2H), 0.42-0.39 (m, 2H); MS (ESI) m/z 567.0 (M+H).

S16-10-133:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.74, 4.71 (s, 1H, total) 4.54, 4.51 (s, 1H, total), 4.10 (s, 1H), 3.09-2.83 (m, 18H), 2.55-2.46 (m, 1H), 2.29-2.26 (m, 1H), 1.67-1.64 (m, 2H), 1.15-1.00 (m, 2H), 0.97-0.83 (m, 2H), 0.79-0.77 (m, 2H), 0.42-10.37 (m, 2H); MS (ESI) m/z 581.1 (M+H).

S16-10-134:

¹H NMR (400 MHz, CD₃OD) δ 7.07, 7.04 (s, 1H, total), 4.94, 4.91, 4.87, 4.84 (s, 1H, total), 4.67, 4.64, 4.55, 4.52 (s, 1H, total), 4.16 (s, 1H), 3.05-2.88 (m, 17H), 2.60-2.52 (m, 1H), 2.35-2.31 (m, 1H), 1.72-1.65 (m, 2H), 1.44 (t, J=14.4, 7.2 Hz, 3H), 1.11-0.75 (m, 6H), 0.51-0.33 (m, 2H); MS (ESI) m/z 595.2

S16-10-135:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (d, J=7.6 Hz, 1H), 4.36-4.32 (m, 1H), 4.17-4.12 (m, 2H), 3.11-2.82 (m, 16H), 2.67-2.63 (m, 1H), 2.48-2.26 (m, 3H), 2.18-2.11 (m, 1H), 1.87-1.42 (m, 7H), 1.21-1.13 (m, 1H); MS (ESI) m/z 581.4 (M+H).

S16-10-136:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (s, 1H), 4.44, 4.41 (s, 1H, total), 4.25, 4.21 (s, 1H, total), 4.13 (s, 1H), 3.47-3.41 (m, 1H), 3.12-2.83 (m, 15H), 2.50-2.47 (m, 2H), 2.32-2.25 (m, 1H), 1.95-1.81 (m, 2H), 1.66-1.60 (m, 3H), 1.45-1.41 (m, 1H), 1.32-1.29 (m, 1H), 1.04 (s, 3H), 0.95, 0.92 (s, 6H, total); MS (ESI) m/z 623.1 (M+H).

S16-10-137:

¹H NMR (400 MHz, CD₃OD) δ 6.84 (s, 1H), 4.74, 4.71, 4.54, 4.50 (s, 1H, total), 4.13, 4.09, 3.97, 3.94 (s, 1H, total), 4.00 (s, 1H), 3.55-3.52 (m, 1H), 3.01-2.55 (m, 18H), 2.55-2.41 (m, 2H), 2.18-2.13 (m, 1H), 1.92-1.85 (m, 1H), 1.75-1.68 (m, 2H), 1.61-1.50 (m, 2H), 1.39-1.25 (m, 2H), 1.17, 0.95 (s, 3H, total), 0.88 (d, J=5.6 Hz, 6H); MS (ESI) m/z 637.3 (M+H).

S16-10-138:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.36 (d, J=12.8 Hz, 1H), 4.13-4.10 (m, 2H), 3.06-2.82 (m, 15H), 2.50-2.38 (m, 1H), 2.29-2.26 (m, 4H), 2.02-1.94 (m, 6H), 1.86-1.72 (m, 6H), 1.68-1.58 (m, 1H); MS (ESI) m/z 621.2 (M+H).

S16-10-139:

¹H NMR (400 MHz, CD₃OD) δ 6.86 (s, 1H), 4.32-4.29 (m, 1H), 4.09-4.05 (m, 2H) 3.02-2.73 (m, 17H), 2.39-2.32 (m, 1H), 2.21-2.18 (m, 1H), 1.69-1.52 (m, 3H) 1.33-1.32 (m, 4H), 0.89-0.86 (m, 3H); MS (ESI) m/z 557.1 (M+H)

S16-10-140:

¹H NMR (400 MHz, CD₃OD) δ 6.94 (s, 1H), 4.41, 4.38 (s, 1H, total), 4.18, 4.15 (s, 1H, total), 4.12 (s, 1H), 3.11-2.81 (m, 17H), 2.51-2.44 (m, 2H), 2.30-2.23 (m, 1H), 2.12-1.95 (m, 5H), 1.70-1.55 (m, 2H), 1.33 (d, J=6.8 Hz, 1H), 1.25 (s, 3H), 1.05 (s, 1H), 1.02 (s, 3H); MS (ESI) m/z 623.2 (M+H).

S16-10-141:

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 1H), 4.70, 4.67 (s, 1H, total), 4.41, 4.14, 4.11 (s, 1H, total), 4.11 (s, 1H), 3.19-2.81 (m, 20H), 2.50-2.45 (m, 2H), 2.29-2.25 (m, 1H), 2.19-2.11 (m, 1H), 2.01-1.92 (m, 5H), 1.70-1.51 (m, 2H), 1.26-1.18 (m, 3H), 1.09-1.02 (m, 2H), 0.91 (s, 1H), 0.80 (s, 1H); MS (ESI) m/z 637.1 (M+H).

S16-10-142:

¹H NMR (400 MHz, CD₃OD) δ 6.98, 6.96 (s, 1H, total), 4.64, 4.60, 4.55, 4.51 (s, 1H, total), 4.29, 4.46, 4.35, 4.22 (s, 1H, total), 4.11 (s, 1H), 3.25-2.82 (m, 19H), 2.59-2.53 (m, 1H), 2.52-2.43 (m, 2H), 2.30-2.23 (m, 1H), 2.19-2.10 (m, 1H), 2.08-1.92 (m, 5H), 1.69-1.50 (m, 2H), 1.29-1.18 (m, 4H), 1.09-1.01 (m, 3H), 0.91 (s, 1H), 0.85 (s, 1H); MS (ESI) m/z 651.2 (M+H).

S16-10-143:

¹H NMR (400 MHz, CD₃OD) δ 6.95-6.85 (m, 1H), 4.65-4.15 (m, 4H), 4.10 (s, 1H), 4.04-3.96 (m, 1H), 3.87-3.80 (m, 1H), 3.05-2.81 (m, 15H), 2.47-2.39 (m, 1H), 2.28-2.25 (m, 1H), 1.69-1.63 (m, 1H), 1.35-1.34 (m, 2H), 1.28-1.26 (m, 2H), 1.21-1.18 (m, 1H); MS (ESI) m/z 541.5 (M+H).

S16-10-144:

¹H NMR (400 MHz, CD₃OD) δ 6.86 (d, J=14.4 Hz, 1H), 4.69-4.53 (m, 2H), 4.40-4.24 (m, 4H), 4.12 (s, 1H), 4.04-3.98 (m, 2H), 3.36 (s, 3H), 3.09-2.82 (m, 15H), 2.48-2.25 (m, 2H), 1.64 (ddd, J=13.6, 11.2, 13.2 Hz, 1H); MS (ESI) m/z 557.0 (M+H).

S16-10-145:

¹H NMR (400 MHz, CD₃OD) δ 7.04 (s, 1H), 4.62-4.59 (m, 1H), 4.36-4.31 (m, 1H), 4.14 (s, 1H), 3.68-3.63 (m, 2H), 3.23-3.17 (m, 2H), 3.14-2.99 (m, 9H), 2.89-2.83 (m, 6H), 2.54-2.44 (m, 1H), 2.37-2.28 (m, 1H), 2.23-2.18 (m, 2H), 2.13-2.07 (m, 2H), 1.71-1.62 (m, 1H); MS (ESI) m/z 541.3 (M+H).

S16-10-146:

¹H NMR (400 MHz, CD₃OD) δ 6.94 (s, 1H), 4.38-4.35 (m, 2H), 4.03 (s, 1H), 3.59-3.52 (m, 1H), 3.47-3.40 (m, 2H), 3.05-2.74 (m, 16H), 2.45-2.32 (m, 1H), 2.34-2.28 (m, 1H), 2.21-2.17 (m, 1H), 2.09-2.05 (m, 1H), 2.00-1.95 (m, 1H), 1.78-1.70 (m, 1H), 1.60-1.57 (m, 1H), 1.43, 1.42 (s, 3H, total); MS (ESI) m/z 555.0 (M+H).

S16-10-147:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (s, 1H), 4.74, 4.71, 4.12, 4.09 (s, 1H, total), 4.10 (s, 1H), 3.65-3.53 (m, 2H), 3.08-2.81 (m, 17H), 2.52-2.40 (m, 1H), 2.41-2.35 (m, 1H), 2.28-2.23 (m, 1H), 2.18-2.10 (m, 1H), 2.08-2.03 (m, 1H), 1.74-1.68 (m, 1H), 1.68-1.60 (m, 1H), 1.49 (d, J=6.4 Hz, 3H); MS (ESI) m/z 555.1 (M+H).

S16-10-148:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (s, 1H), 4.72, 4.68 (s, 1H, total), 4.21 (s, 1H), 4.12, 4.09 (s, 1H, total), 3.87-3.80 (m, 1H), 3.45-3.41 (m, 7H), 3.16-3.01 (m, 10H), 2.75-2.69 (m, 2H), 2.49-2.44 (m, 1H), 2.40-2.38 (m, 1H), 2.18-2.07 (m, 2H), 1.73-1.65 (m, 1H); MS (ESI) m/z 609.0 (M+H).

S16-10-149:

¹H NMR (400 MHz, CD₃OD) δ 6.86 (s, 1H), 4.48 (d, J=12.8 Hz, 1H), 4.28 (d, J=13.2 Hz, 1H), 4.02 (s, 1H), 3.77-3.42 (m, 5H), 3.36 (s, 3H), 3.13-2.70 (m, 15H), 2.39-2.32 (m, 1H), 2.24-2.19 (m, 2H), 2.16-2.07 (m, 1H), 2.05-1.87 (m, 2H), 1.61-1.49 (m, 1H); MS (ESI) m/z 585.0 (M+H).

S16-10-150:

¹H NMR (400 MHz, CD₃OD) δ 6.83 (s, 1H), 4.03 (s, 2H), 3.83-3.64 (m, 2H), 3.63-3.53 (m, 2H), 3.51-3.46 (m, 1H), 3.43 (s, 3H), 3.41-3.35 (m, 1H), 3.12-2.63 (m, 15H), 2.50-2.35 (m, 1H), 2.34-2.14 (m, 2H), 2.13-1.83 (m, 3H), 1.68-1.43 (m, 1H); MS (ESI) m/z 585.0 (M+H).

S16-10-151:

¹H NMR (400 MHz, CD₃OD) δ 6.93 (s, 1H), 5.46, 5.33 (s, 1H, total), 4.58 (d, J=14.2 Hz, 1H), 4.34 (d, J=14.2 Hz, 1H), 4.04 (s, 1H), 3.85-3.61 (m, 2H), 3.40-3.39 (m, 1H), 3.01-2.75 (m, 16H), 2.43-2.18 (m, 4H), 1.59-1.56 (m, 1H), MS (ESI) m/z 559.3 (M+H).

S16-10-152:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 5.55, 5.40 (s, 1H, total), 4.63, 4.60 (s, 1H, total), 4.39-4.31 (m, 1H), 4.10 (s, 1H), 3.89-3.76 (m, 2H), 3.08-2.82 (m, 17H), 2.51-2.35 (m, 3H), 2.32-2.23 (m, 1H), 1.69-1.57 (m, 1H); MS (ESI) m/z 559.2 (M+H)

S16-10-153:

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 1H), 4.64, 4.61 (s, 1H, total), 4.29, 4.26 (s, 1H, total), 4.13 (s, 1H), 3.7 (t, J=23.2, 11.6 Hz, 2H), 3.59-3.51 (m, 2H), 3.15-2.96 (m, 15H), 2.69-2.60 (m, 2H), 2.55-2.42 (m, 1H), 2.33-2.30 (m, 1H), 1.66-1.63 (m, 1H); MS (ESI) m/z 577.0 (M+H).

S16-10-154:

¹H NMR (400 MHz, CD₃OD) δ 7.03, 7.02 (s, 1H, total), 4.63-4.55 (m, 1H), 4.33 (t, J=28.8, 14.4 Hz, 1H), 4.12 (s, 1H), 3.69-3.66 (m, 1H), 3.42-3.39 (m, 1H), 3.05-2.83 (m, 17H), 2.51-2.40 (m, 1H), 2.29-2.26 (m, 1H), 2.05-2.01 (m, 1H), 1.99-1.92 (m, 1H), 1.66-1.56 (m, 5H), 0.92-0.86 (m, 6H); MS (ESI) m/z 597.1 (M+H).

S16-10-155:

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.63, 4.60 (s, 1H, total), 4.32, 4.29 (s, 1H, total), 4.11 (s, 1H), 3.74-3.71 (m, 2H), 3.06-2.86 (m, 15H), 2.49-3.38 (m, 1H), 2.32-2.26 (m, 3H), 1.83-1.81 (m, 2H), 1.69-1.60 (m, 1H), 1.39, 1.38 (s, 3H, total), 1.31, 1.30 (s, 3H, total); MS (ESI) m/z 569.1 (M+H).

S16-10-156:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (s, 1H), 4.48-4.22 (m, 2H), 4.11 (s, 1H), 3.92-3.81 (m, 1H), 3.52-3.42 (m, 1H), 3.22-2.68 (m, 17H), 2.63-2.51 (m, 1H), 2.50-2.41 (m, 1H), 2.31-2.22 (m, 1H), 2.21-2.10 (m, 1H), 1.94 (s, 3H), 1.64-1.58 (m, 1H); MS (ESI) m/z 598.2 (M+H).

S16-10-157:

¹H NMR (400 MHz, CD₃OD) δ 6.91 (s, 1H), 4.73-4.69 (m, 1H), 4.22 (s, 1H), 4.10 (s, 1H), 3.79-3.74 (m, 1H), 3.50-3.40 (m, 1H), 3.20-2.88 (m, 17H), 2.61-2.41 (m, 2H), 2.31-2.22 (m, 1H), 2.19-2.08 (m, 1H), 1.95 (s, 3H), 1.66-1.57 (m, 1H); MS (ESI) m/z 598.2 (M+H).

S16-10-158:

¹H NMR (400 MHz, CD₃OD) δ 7.02, 7.00 (s, 1H, total), 4.55 (t, J=22.8, 13.2 Hz, 1H), 4.32, 4.29, 4.24, 4.21 (s, 1H, total), 4.12 (s, 1H), 3.45-3.41 (m, 1H), 3.20-3.15 (m, 1H), 3.10-2.75 (m, 19H), 2.49-2.41 (m, 1H), 2.28-2.23 (m, 1H), 1.85-1.53 (m, 7H); MS (ESI) m/z 581.1 (M+H).

S16-10-159:

¹H NMR (400 MHz, CD₃OD) δ 6.95 (s, 1H), 4.58-4.53 (m, 1H), 4.32-4.29 (m, 1H), 4.03 (s, 1H), 3.58-3.39 (m, 3H), 3.12-2.75 (m, 16H), 2.57-2.33 (m, 3H), 2.20-2.17 (m, 1H), 1.65-1.33 (m, 9H); MS (ESI) m/z 595.2 (M+H).

S16-10-160:

¹H NMR (400 MHz, CD₃OD) δ 7.32 (s, 4H), 6.98 (s, 1H), 4.76-4.43 (m, 6H), 4.05 (s, 1H), 3.01-2.73 (m, 15H), 2.44-2.36 (m, 1H), 2.21-2.18 (m, 1H), 1.62-1.53 (m, 1H); MS (ESI) m/z 589.0 (M+H).

S16-10-161:

¹H NMR (400 MHz, CD₃OD) δ 7.06 (s, 1H), 4.54-4.47 (m, 1H), 4.29-4.26 (m, 1H), 4.14 (s, 1H), 3.51-3.44 (m, 2H), 3.10-2.98 (m, 11H), 2.89-2.83 (m, 6H), 2.51-2.43 (m, 1H), 2.31-2.27 (m, 1H), 1.97-1.92 (m, 2H), 1.88-1.81 (m, 3H), 1.69-1.52 (m, 2H); MS (ESI) m/z 555.2 (M+H).

S16-10-162:

¹H NMR (400 MHz, CD₃OD) δ 7.04, 7.03 (s, 1H, total), 4.50 (d, J=13.2 Hz, 1H), 4.25 (d, J=13.2 Hz, 1H), 4.11 (s, 1H), 3.51-3.42 (m, 2H), 3.20-2.81 (m, 17H), 2.49-2.41 (m, 1H), 2.29-2.25 (m, 1H), 1.93-1.90 (m, 2H), 1.73-1.62 (m, 2H), 1.60-1.42 (m, 2H), 1.01 (d, J=6.4 Hz, 3H); MS (ESI) m/z 569.0 (M+H)

S16-10-163:

¹H NMR (400 MHz, CD₃OD) δ 6.94, 6.93 (s, 1H, total), 4.40 (d, J=12.8 Hz, 1H), 4.16 (d, J=13.2 Hz, 1H), 3.98 (s, 1H), 3.52-3.49 (m, 1H), 3.42-3.39 (m, 1H), 3.07-2.67 (m, 18H), 2.36-2.28 (m, 1H), 2.15-2.12 (m, 1H), 2.02 (m, 2H), 1.77-1.74 (m, 2H), 1.55-1.49 (m, 1H); MS (ESI) m/z 623.0 (M+H).

S16-10-164:

¹H NMR (400 MHz, CD₃OD) δ 7.04 (s, 1H), 4.49 (d, J=13.2 Hz, 1H), 4.24 (d, J=13.2 Hz, 1H), 4.12 (s, 1H), 3.54-3.47 (m, 2H), 3.05-2.80 (m, 17H), 2.43-2.38 (m, 1H), 2.28-2.25 (m, 1H), 2.01-1.96 (m, 2H), 1.67-1.64 (m, 1H), 1.55-1.41 (m, 4H), 0.99 (d, J=6.8 Hz, 6H); MS (ESI) m/z 597.3 (M+H).

S16-10-165:

¹H NMR (400 MHz, CD₃OD) δ 7.06 (s, 1H), 4.52, 4.49 (s, 1H, total), 4.28, 4.24 (s, 1H, total), 4.13 (s, 1H), 3.59-3.49 (m, 2H), 3.41-3.35 (m, 1H), 3.07-2.82 (m, 16H), 2.50-2.41 (m, 1H), 2.30-2.27 (m, 1H), 2.02-1.98 (m, 2H), 1.68-1.60 (m, 3H), 1.46-1.40 (m, 1H), 0.98 (s, 9H); MS (ESI) m/z 611.1 (M+H).

S16-10-166:

¹H NMR (400 MHz, CD₃OD) δ 6.95 (s, 1H), 4.42 (d, J=13.2 Hz, 1H), 4.19 (d, J=14.0 Hz, 1H), 4.03 (s, 1H), 3.45-3.35 (m, 2H), 3.05-2.70 (m, 17H), 2.40-2.29 (m, 1H), 2.20-2.13 (m, 1H), 1.93-1.85 (m, 2H), 1.59-1.50 (m, 2H), 1.39-1.18 (m, 6H), 0.88-0.79 (m, 3H); MS (ESI) m/z 597.1 (M+H).

S16-10-167:

¹H NMR (400 MHz, CD₃OD) δ 6.93, 6.89 (s, 1H, total), 4.52, 4.48, 4.46, 4.41 (s, 1H, total), 4.27, 4.24, 4.18, 4.14 (s, 1H, total), 4.01 (s, 1H), 2.98-2.70 (m, 18H), 2.43-2.38 (m, 1H), 2.18-2.13 (m, 1H), 1.95-1.93 (m, 1H), 1.80-1.74 (m, 2H), 1.65-1.58 (m, 4H), 1.45 (d, J=6.4 Hz, 2H), 1.39 (d, J=6.8 Hz, 1H); MS (ESI) m/z 569.1 (M+H).

S16-10-168:

¹H NMR (400 MHz, CD₃OD) δ 7.23-7.17 (m, 5H), 7.02 (s, 1H), 4.51-4.47 (m, 1H), 4.26-4.22 (m, 1H), 4.03 (s, 1H), 3.58-3.47 (m, 2H), 3.15-2.75 (m, 17H), 2.59-2.55 (m, 1H), 2.42-2.35 (m, 1H), 2.21-2.18 (m, 1H), 2.01-1.94 (m, 3H), 1.62-1.52 (m, 1H), 1.20-1.18 (m, 1H); MS (ESI) m/z 631.1 (M+H).

S16-10-169:

¹H NMR (400 MHz, CD₃OD) δ 7.05, 7.04 (s, 1H, total), 4.56-4.55 (m, 1H), 4.30-4.23 (m, 1H), 4.10 (s, 1H), 4.07, 3.87 (m, 1H, total), 3.57-3.45 (m, 1H), 3.38-3.33 (m, 2H), 3.19-2.81 (m, 15H), 2.52-2.40 (m, 1H), 2.29-2.23 (m, 1H), 2.19-2.13 (m, 1H), 2.07-2.85 (m, 2H), 1.79-1.57 (m, 2H); MS (ESI) m/z 571.0 (M+H).

S16-10-170:

¹H NMR (400 MHz, CD₃OD) δ 7.05, 7.03 (s, 1H total), 4.53 (d, J=13.2 Hz, 1H), 4.28 (d, J=13.2 Hz, 1H), 4.11 (s, 1H), 3.40-3.37 (m, 2H), 3.16-2.81 (m, 17H), 2.51-2.43 (m, 1H), 2.28-2.25 (m, 1H), 1.90-1.79 (m, 4H), 1.71-1.61 (m, 1H), 1.29 (s, 3H); MS (ESI) m/z 585.1 (M+H).

S16-10-171:

¹H NMR (400 MHz, CD₃OD) δ 7.09 (s, 1H), 4.55-4.51 (m, 1H), 4.36-4.28 (m, 1H), 4.14 (s, 1H), 3.64 (s, 1H), 3.58-3.51 (m, 1H), 3.38 (s, 3H), 3.18-2.84 (m, 15H), 2.51-2.42 (m, 1H), 2.31-2.28 (m, 2H), 2.18-2.13 (m, 1H), 2.05-1.95 (m, 2H), 1.78-1.62 (m, 2H); MS (ESI) m/z 585.1 (M+H).

S16-10-172:

¹H NMR (400 MHz, CD₃OD) δ 6.95 (s, 1H), 4.44-4.40 (m, 1H), 4.19-4.15 (m, 1H), 4.03 (s, 1H), 3.47-3.34 (m, 2H), 3.23 (s, 3H), 3.01-2.71 (m, 19H), 2.41-2.33 (m, 1H), 2.20-2.17 (m, 1H), 1.92-1.81 (m, 3H), 1.61-1.44 (m, 3H); MS (ESI) m/z 599.3 (M+H).

S16-10-180:

¹H NMR (400 MHz, CD₃OD) δ 6.93 (s, 1H), 4.60 (d, J=13.6 Hz, 1H), 4.22 (d, J=13.6 Hz, 1H), 4.09 (s, 1H), 3.24-2.86 (m, 19H), 2.52-2.42 (m, 1H), 2.30-2.20 (m, 1H), 1.71-1.55 (m, 4H), 1.41-1.31 (m, 3H), 1.03-0.99 (m, 6H); MS (ESI) m/z 571.2 (M+H).

S16-10-174:

¹H NMR (400 MHz, CD₃OD) δ 7.03 (s, 1H), 4.51 (d, J=13.2 Hz, 1H), 4.25 (d, J=13.2 Hz, 1H), 4.11 (s, 1H), 3.40-3.37 (m, 2H), 3.05-2.80 (m, 17H), 2.68-2.57 (m, 2H), 2.47-2.43 (m, 1H), 2.28-2.25 (m, 1H), 1.89-1.85 (m, 2H), 1.71-1.61 (m, 1H), 0.99 (d, J=3.6 Hz, 3H), 0.98 (d, J=3.6 Hz, 3H); MS (ESI) m/z 583.3

S16-10-175:

¹H NMR (400 MHz, CD₃OD) δ 7.06 (s, 1H), 4.87 (d, J=20.0 Hz, 1H), 4.17 (d, J=13.6 Hz, 1H), 4.13-4.11 (m, 2H), 4.09 (s, 1H), 3.09-2.80 (m, 15H), 2.51-2.40 (m, 1H), 2.31-2.23 (m, 3H), 2.18-2.10 (m, 2H), 1.92-1.85 (m, 4H), 1.58-1.53 (m, 1H); MS (ESI) m/z 567.1 (M+H).

S16-10-176:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (s, 1H), 4.56 (d, J=13.2 Hz, 1H), 4.30 (d, J=13.2 Hz, 1H), 4.11 (s, 1H), 4.05-4.00 (m, 2H), 3.90-3.76 (m, 2H), 3.42-3.30 (m, 2H), 3.24-3.16 (m, 2H), 3.07-2.84 (m, 15H), 2.52-2.41 (m, 1H), 2.29-2.25 (m, 1H), 1.68-1.58 (m, 1H); MS (ESI) m/z 557.1 (M+H).

S16-10-177:

¹H NMR (400 MHz, CD₃OD) δ 7.03 (s, 1H), 4.61, 4.58 (s, 1H, total), 4.33, 4.30 (s, 1H, total), 4.13 (s, 1H), 3.53-3.49 (m, 2H), 3.29-3.26 (m, 2H), 3.09-2.85 (m, 15H), 2.49-2.41 (m, 1H), 2.32-2.27 (m, 1H), 2.09-1.95 (m, 2H), 1.95-1.83 (m, 2H), 1.68-1.65 (m, 4H), 1.65-1.60 (m, 1H); MS (ESI) m/z 569 (M+H).

S16-10-178:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (s, 1H), 4.73-4.70 (m, 2H), 4.10 (s, 1H), 3.52-3.43 (m, 2H), 3.09-2.72 (m, 16H), 2.47-2.33 (m, 2H), 2.29-2.21 (m, 1H), 2.17-1.98 (m, 3H), 1.86-1.75 (m, 1H), 1.63-1.49 (m, 2H), 1.05 (t, J=7.2 Hz, 3H); MS (ESI) m/z 569.1 (M+H).

S16-10-179:

¹H NMR (400 MHz, CD₃OD) δ 7.02 (s, 1H), 4.51, 4.49 (s, 1H, total), 4.44, 4.41 (s, 1H, total), 4.13 (s, 1H), 3.55-3.49 (m, 2H), 3.06-2.81 (m, 16H), 2.46-2.41 (m, 2H), 2.30-2.25 (m, 1H), 2.19-2.01 (m, 3H), 1.85-1.79 (m, 1H), 1.70-1.6 (m, 1H), 1.02 (t, J=7.2 Hz, 3H); MS (ESI) m/z 569.1 (M+H).

Example 17. Synthesis of Compounds Via Scheme 17

The following compounds were prepared according to Scheme 17.

n-BuLi (5.0 mL, 1.62 M/hexanes, 8.12 mmol, 1.3 equiv) was added dropwise to a THF solution (30 mL) of S15-5 (2.0 g, 6.24 mmol, 1.0 equiv) at −100° C. The reaction was stirred at −100° C. for 1 min. DMF (2.0 mL, 25.00 mmol, 4.0 equiv) was added. The reaction was allowed to warm to −78° C. and stirred at −78° C. for 1 h. The reaction mixture was further warmed to 0° C. and quenched by saturated aqueous NH₄Cl. The resulting mixture was extracted with EtOAc. The combined EtOAc extracts were dried (sodium sulfate) and concentrated to give crude S17-1.

NaBH₄ (0.28 g, 7.40 mmol, 1.2 equiv) was added to a solution of crude S17-1 (6.24 mmol, 1.0 equiv) in MeOH (15 mL) at 0° C. The reaction was stirred at 0° C. for 30 min and quenched by H₂O. The resulting mixture was extracted with EtOAc. The combined EtOAc extracts were dried (sodium sulfate) and concentrated to give crude S17-2.

N-Bromosuccinimide (1.32 g, 7.44 mmol, 1.2 equiv) was added to a solution of crude S17-2 (6.24 mmol, 1.0 equiv) in dichloromethane (20 mL). The reaction was stirred at 25° C. for 2 hrs. Saturated aqueous Na₂SO₃ was added to the reaction. The resulting mixture was extracted with dichloromethane. The combined dichloromethane extracts were dried (sodium sulfate) and concentrated to give the crude S17-3.

BBr₃ (13.4 mL, 1.0 M/dichloromethane, 13.40 mmol, 2.0 equiv) was added to a solution of S17-3 dichloromethane (20 mL) at −78° C. The reaction was stirred from −78° C. to 25° C. for 1.5 hrs, quenched by saturated aqueous NaHCO₃ and concentrated under reduced pressure to remove most of the dichloromethane. EtOAc and H₂O were added to the residue. The aqueous layer was extracted with EtOAc. The combined EtOAc extracts were dried (sodium sulfate) and concentrated to yield crude phenol.

Potassium carbonate (1.7 g, 12.40 mmol, 2.0 equiv) and benzylbromide (1.5 mL, 12.40 mmol, 2.0 equiv) were added to a solution of the above crude phenol in acetone at 25° C. The reaction was stirred at 25° C. for 12 hrs. The reaction mixture was concentrated and the residue was redissolved in H₂O and EtOAc. The aqueous layer was extracted with EtOAc. The combined organic layers were dried (sodium sulfate) and concentrated to give crude S17-4. Flash chromatography on silica gel (10:1 to 3:1 hexanes/EtOAc) yielded 1.97 g of compound S-17-4 (72% for 5 steps).

Dess-Marlin periodinane (0.18 g, 0.42 mmol, 1.2 equiv) was added to a solution of S17-4 (0.15 g, 0.35 mmol, 1.0 equiv) in dichloromethane (2 mL) at 25° C. The reaction was stirred at 25° C. for 30 min and diluted with H₂O. The resulting mixture was extracted with dichloromethane. The combined dichloromethane extracts were dried (sodium sulfate) and concentrated to give crude S17-5.

4,4-Dimethylpiperidine hydrochloride (0.11 g, 0.70 mmol, 2.0 equiv) and Et₃N (96 μL, 0.70 mmol, 2.0 equiv) were added to a solution of crude S17-5 (0.35 mmol, 1.0 equiv) in dichloromethane at 25° C. The reaction was stirred at 25° C. for 15 min. Na(OAc)₃BH (0.22 g, 1.05 mmol, 3.0 equiv) and HOAc (0.1 mL) were added to the reaction. The reaction was stirred at 25° C. for 1 h and quenched by H₂O. The resulting mixture was extracted with dichloromethane. The combined dichloromethane extracts were dried (sodium sulfate) and concentrated under reduced pressure. Flash chromatography on silica gel (10:1 to 4:1 hexanes/EtOAc) yielded 95 mg of compound S17-6-1 (52% for 2 steps).

n-BuLi (0.55 mL, 1.6 M/hexanes, 0.88 mmol, 1.4 equiv) was added dropwise to a solution of S17-6-1 (0.33 g, 0.63 mmol, 1.0 equiv) in THF (2 mL) at −100° C. The reaction was stirred at −100° C. for 1 min. TsCN (0.36 g, 1.89 mmol, 3.0 equiv) was added to the reaction mixture. The reaction was allowed to warm to −78° C. and stirred at −78° C. for 1 h. The reaction mixture was further warmed to 0° C. and quenched by saturated aqueous NH₄Cl. The resulting mixture was extracted with EtOAc. The combined EtOAc extracts were dried (sodium sulfate) and concentrated under reduced pressure. Flash chromatography on silica gel (4:1 hexanes/EtOAc) yielded 28 mg of compound S17-7-1 (10%).

LHMDS (0.24 mL, 1.0 M/THF, 0.24 mmol, 4.0 equiv) was added to a solution of S17-7-1 (28 mg, 0.060 mmol, 1.2 equiv), TMEDA (54 μL, 0.36 mmol, 7.2 equiv) and enone S1-9 (23 mg, 0.050 mmol, 1.0 equiv) in THF (1 mL) at −78° C. The reaction was stirred at −78° C. for 30 min, allowed to warm to 25° C. over 1 h. quenched by saturated NH₄Cl solution, and extracted with EtOAc. The combined EtOAc extracts were dried (sodium sulfate) and concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 4.0 mL (CH₃CN); gradient: 50→100% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product were collected and concentrated at 25° C. to remove most of the acetonitrile. The resulting aqueous solution was extracted with EtOAc. The combined EtOAc extracts were dried (sodium sulfate) and concentrated to give 8 mg of S17-8-1 (16%).

Aqueous HF (0.3 mL, 48-50%) was added to a solution of S17-8-1 (8 mg, 0.0093 mmol) in acetonitrile (2 mL) in a polypropylene tube at 25° C. The reaction was stirred at 25° C. for 18 hrs. The resulting mixture was poured into an aqueous solution of K₂HPO₄ (2 g, dissolved in 15 mL water) and extracted with EtOAc. The combined EtOAc extracts were dried (sodium sulfate) and concentrated to yield the crude desilylated intermediate.

Pd—C (8 mg, 10 wt %) was added to a solution of the above crude product in HCl/MeOH (2 mL, 0.5 N). The reaction was purged with hydrogen and stirred under H₂ (balloon) at 25° C. for 4 hrs. The reaction mixture was filtered through a small Celite plug. The filtrate was concentrated to yield the crude product, which was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 0→70% B over 7 min, 70→100% over 3 min, and 100% over 5 min; mass-directed fraction collection]. Fractions containing the desired product were collected and freeze-dried to yield 1 mg of compound S17-9-1: ¹H NMR (400 MHz, CD₃OD) δ 7.28 (s, 1H), 4.52 (s, 2H), 4.12 (s, 1H), 3.52-3.40 (m, 2H), 3.20-2.93 (m, 5H), 3.04 (s, 3H), 2.95 (s, 3H), 2.78-2.67 (m, 1H), 2.30-2.23 (m, 1H), 1.80-1.58 (m, 5H), 1.11 (s, 3H), 1.04 (s, 3H); MS (ESI) m/z 565.23 (M+H).

The following compound was prepared similarly to S17-9-1.

S17-9-2:

¹H NMR (400 MHz, CD₃OD) δ 7.28 (s, 1H), 4.16 (s, 1H), 4.13 (s, 1H), 3.24-2.90 (m, 3H), 3.05 (s, 3H), 2.96 (m, 3H), 2.78 (m, 3H), 2.83-2.66 (m, 1H), 2.32-2.24 (m, 1H), 1.74-1.60 (m, 1H), 1.58 (s, 9H); MS (ESI) m/z 539.25 (M+H).

Example 18. Synthesis of Compounds Via Scheme 18

The following compounds were prepared according to Scheme 18.

A solution of benzoic acid S18-1 (17.51 g, 102.90 mmol, 1.0 equiv) in THF (150 mL) was added dropwise to a solution of s-BuLi (161.70 mL, 1.4 M/cyclohexane, 226.40 mmol, 2.2 equiv) and TMEDA (34.00 mL, 226.40 mmol, 2.2 equiv) in THF (150 mL) at −78° C. via a cannula over 1 h 30 min. The resulting orange reaction mixture was stirred at −78° C. for 2 hrs. Iodomethane (8.38 mL, 134.30 mmol, 5.0 equiv) was added at −78° C. while manually shaking the reaction mixture. The dry-ice/acetone bath was removed, and the resulting white suspension was then stirred at rt for 30 min. Water (150 mL) was added. The resulting purple mixture was concentrated to remove most of the THF and other organic volatiles. Aqueous NaOH (6 M, 100 mL) was added. The mixture was extracted with methyl t-butyl ether (2×200 mL). The aqueous layer was acidified with aqueous HCl (170 mL, 6 N) to pH 1, and extracted with EtOAc (150 mL, then 2×80 mL). The combined organic phase was dried over magnesium sulfate, filtered and concentrated to afford the crude product as an orange solid (15.03 g, containing the desired product S18-2, the regioisomer (i.e. 2-methoxy-3-methyl-4-fluoro benzoic acid) and starting material S18-1 at a ratio of 1.7:3:0.9 as indicated by ¹H NMR analysis).

The above crude product was dissolved in dichloromethane (50 mL). Oxalyl chloride (2.66 mL, 30.44 mmol, 1.1 equiv) was added at rt followed by a couple of drops of DMF. The mixture was stirred at rt for 1 h and the solvent was evaporated. The residue was dried under high vacuum. The resulting orange oil was re-dissolved in dichloromethane (50 mL). Phenol (2.86 g, 30.44 mmol, 1.1 equiv), triethylamine (7.70 mL, 55.35 mmol, 2.0 equiv), and DMAP (catalytic amount) were added. The reaction mixture was stirred at rt for 1 h. Solvents were evaporated and the residue was dissolved in EtOAc (150 mL) and water (50 mL). The organic layer was washed with aqueous HCl (50 mL, 1 N), 1 N aqueous NaOH (2×30 mL) and brine (50 mL), dried over anhydrous magnesium sulfate, filtered and concentrated. The residue was purified by flash column chromatography (1-4% EtOAc/hexanes) to afford the desired product S18-3 as a yellow solid (2.73 g, 41% over five steps): ¹H NMR (400 MHz, CDCl₃) δ 7.45-7.41 (m, 2H), 7.29-7.22 (m, 3H), 6.59-6.53 (m, 2H), 3.87 (s, 3H), 2.43 (s, 3H); MS (ESI) m/z 259.15 (M+H).

A solution of BBr₃ in dichloromethane (10.49 mL, 1.0 M, 10.49 mmol, 1.0 equiv) was added slowly to a solution of the above compound S18-3 (2.73 g, 10.49 mmol, 1.0 equiv) in dichloromethane (50 mL) at −78° C. The resulting orange solution was stirred at −78° C. for 10 min and then at 0° C. for 15 min. Saturated aqueous NaHCO₃ (30 mL) was added slowly. The resulting mixture was stirred at rt for 5 min and the dichloromethane was evaporated. The residue was extracted with EtOAc (80 mL, then 30 mL). The organic extracts were combined, dried over anhydrous magnesium sulfate, filtered, and concentrated to afford an orange solid, which was used directly in the next reaction.

Di-t-butyl dicarbonate (2.40 g, 11.01 mmol, 1.05 equiv) and N,N-dimethylaminopyridine (catalytic amount) were added to a solution of the above product in dichloromethane (50 mL). The reaction was stirred for 40 min at rt (monitored by LC-MS and TLC (product is slightly more polar)), and concentrated. The residue was purified by flash column chromatography (1-5% EtOAc/hexanes containing 10% dichloromethane) to afford product S18-4 as a white solid (3.45 g, 95% for two steps): ¹H NMR (400 MHz, CDCl₃) δ 7.46-7.42 (m, 2H), 7.31-7.24 (m, 3H), 6.94-6.88 (m, 2H), 2.54 (s, 3H), 1.45 (s, 9H); MS (ESI) m/z 345.25 (M+H).

A solution of ester S18-4 (0.50 g, 1.45 mmol, 1.0 equiv) in THF (2.5 mL) was added to a solution of LDA (1.87 mL, 1.55 M/THF/heptane/ethylbenzene, 2.90 mmol, 2.0 equiv) and TMEDA (0.44 mL, 2.90 mmol, 2.0 equiv) in THF (20 mL) via a cannula. The reaction mixture was stirred at −78° C. for 15 min. A solution of enone S1-9 (0.49 g, 1.01 mmol, 0.7 equiv) in THF (2.5 mL) was added to the reaction mixture via a cannula. The reaction mixture was then allowed to warm to 0° C. over 1 h, quenched by saturated aqueous NH₄Cl, and extracted with EtOAc (70 mL). The combined organic extracts were dried (sodium sulfate), filtered and concentrated. The residue was purified by flash column chromatography (5-10% EtOAc/hexanes) to yield the desired product S18-5 (0.28 g, 37%): ¹H NMR (400 MHz, CDCl₃) δ 13.97 (br s, 1H), 7.49-7.47 (m, 2H), 7.39-7.32 (m, 3H), 6.44 (d, J=9.2 Hz, 1H), 5.35 (s, 2H), 3.86 (d, J=10.4 Hz, 1H), 3.08-3.00 (m, 1H), 2.94-2.90 (m, 1H), 2.81-2.73 (m, 1H), 2.46-2.41 (m, 9H), 1.56 (s, 9H), 0.83 (s, 9H), 0.27 (s, 3H), 0.12 (s, 3H); MS (ESI) m/z 735.41 (M+H). Some unreacted enone S1-9 (0.17 g) was also recovered.

Aqueous HF (48-50%, 0.4 mL) was added to a solution of compound S18-5 (0.28 g, 0.37 mmol) in acetonitrile (1.2 mL) in a polypropylene reaction vessel at 23° C. The resulting mixture was stirred vigorously at 23° C. overnight and poured into aqueous K₂HPO₄ (4.8 g dissolved in 30 mL water). The mixture was extracted with 5% MeOH in EtOAc (4×60 mL). The combined organic extracts were dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 3.0 mL (CH₃CN); gradient: 0→100% B in A over 10 min; mass-directed fraction collection] to yield S18-6-1 and S18-6-2.

S18-6-1

(54 mg, 23%): ¹H NMR (400 MHz, CD₃OD) δ 14.49 (br s, 1H), 12.45 (br s, 1H), 7.46-7.41 (m, 2H), 7.38-7.30 (m, 3H), 6.37 (d, J=9.2 Hz, 1H), 5.34 (s, 2 H), 3.78 (br s, 1H), 2.96-2.93 (m, 2H), 2.72-2.67 (m, 1H), 2.61-2.46 (m, 7H), 2.05-2.02 (m, 1H), 1.56 (s, 9H); MS (ESI) m/z 621.19 (M+H).

S18-6-2

(0.16 g, 77%): ¹H NMR (400 MHz, CD₃OD) δ 7.40-7.38 (m, 2H), 7.32-7.26 (m, 3H), 6.53 (d, J=9.6 Hz, 1H), 5.32, 5.28 (Abq, J=11.9 Hz, 2H), 3.35-3.31 (m, 1H), 3.25-2.98 (m, 9H), 2.76-2.71 (m, 1H), 2.44 (t, J=14.6 Hz, 1H), 2.06-2.02 (m, 1H); MS (ESI) m/z 565.18 (M+H).

Pd—C (10 wt %, 15 mg) was added in one portion into a solution of compound S18-6-1 (54 mg, 0.087 mmol) in a mixture of MeOH (3 mL) and dioxane (0.5 mL) at 23° C. The reaction vessel was sealed and purged with hydrogen by briefly evacuating the flask followed by flushing with hydrogen gas (1 atm). The resulting mixture was stirred at 23° C. for 50 min. The reaction mixture was then filtered through a small Celite pad. The filtrate was concentrated. The residue was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 20→80% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 6.7-7.6 min, were collected and freeze-dried to yield compound S18-7-1 (29 mg, 59%): ¹H NMR (400 MHz, CD₃OD) δ 6.62 (d, J=9.6 Hz, 1H), 4.09 (s, 1H), 3.07-2.94 (m, 8H), 2.90-2.85 (m, 1H), 2.57 (t, J=14.6 Hz, 1H), 2.21-2.16 (m, 1H), 1.61-1.57 (m, 1H), 1.57 (s, 9H); MS (ESI) m/z 533.25 (M+H).

Compound S18-7-2 was prepared similarly.

S18-7-2:

¹H NMR (400 MHz, CD₃OD) δ 6.65 (d, J=10.1 Hz, 1H), 4.09 (s, 1H), 3.10-2.94 (m, 8H), 2.89 (dd, J=4.1, 15.6 Hz, 1H), 2.58 (t, J=14.6 Hz, 1H), 2.22-2.17 (m, 1H), 1.63-1.54 (m, 1H); MS (ESI) m/z 477.16 (M+H).

A mixture of compound S18-6-2 (27 mg, 0.048 mmol, 1.0 equiv), EDC (18 mg, 0.095 mmol, 2.0 equiv) and HOBt (3 mg, 0.024 mmol, 0.5 equiv) were dissolved in DMF (0.5 mL). Neopentylamine (11 μL, 0.095 mmol, 2.0 equiv) was added. The reaction mixture was stirred at it overnight and filtered through a cotton plug. The cotton plug was washed with acetonitrile (1 mL). The filtrate was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 3.0 mL (CH₃CN); gradient: 0→60% B in A over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 7.8-8.7 min, were collected and freeze-dried to yield compound S18-8-1 (14 mg, 46%): ¹H NMR (400 MHz, CDCl₃) δ 7.50-7.47 (m, 2H), 7.42-7.36 (m, 3H), 6.62 (d, J=9.6 Hz, 1H), 5.42, 5.38 (Abq, J=11.9 Hz, 2H), 4.82 (d, J=1.4 Hz, 1H), 3.41-3.38 (m, 1H), 3.20-3.16 (m, 2H), 3.16 (s, 3H), 3.09-3.01 (m, 1H), 2.83 (dd, J=4.1, 15.6 Hz, 1H), 2.54 (t, J=14.6 Hz, 1H), 2.13-2.08 (m, 1H), 1.42-1.32 (m, 1H), 0.98 (s, 9H); MS (ESI) m/z 634.26 (M+H).

Pd—C (10 wt %, 5 mg) was added in one portion into a solution of compound S18-8-1 (14 mg, 0.022 mmol) in MeOH (3 mL) at 23° C. The reaction vessel was sealed and purged with hydrogen by briefly evacuating the flask followed by flushing with hydrogen gas (1 atm). The reaction mixture was stirred at 23° C. for 30 min. The reaction mixture was then filtered through a small Celite pad. The filtrate was concentrated. The residue was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 15→70% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 7.1-8.8 min, were collected and freeze-dried to yield compound S18-9-1 (8 mg, 64%): ¹H NMR (400 MHz, CD₃OD) δ 6.64 (d, J=9.2 Hz, 1H), 4.08 (s, 1H), 3.18 (s, 2H), 3.10-2.95 (m, 8H), 2.91-2.86 (m, 1H), 2.57 (t, J=14.6 Hz, 1H), 2.20-2.17 (m, 1H), 1.63-1.54 (m, 1H), 0.98 (s, 9H); MS (ESI) m/z 546.23 (M+H).

The following compounds were prepared similarly to S18-8-1 or S18-9-1.

S18-8-2:

¹H NMR (400 MHz, CDCl₃) δ 7.49-7.47 (m, 2H), 7.42-7.36 (m, 3H), 6.66 (d, J=9.6 Hz, 1H), 5.42, 5.38 (Abq, J=11.9 Hz, 2H), 4.81 (d, J=1.4 Hz, 1H), 3.60 (t, J=6.9 Hz, 2H), 3.41-3.32 (m, 3H), 3.16 (s, 6H), 3.11-3.02 (m, 1H), 2.83 (dd, J=4.1, 15.6 Hz, 1H), 2.56 (t, J=15.1 Hz, 1H), 2.11-2.07 (m, 1H), 2.03-1.90 (m, 4H), 1.43-1.33 (m, 1H); MS (ESI) m/z 618.26 (M+H).

S18-9-2:

¹H NMR (400 MHz, CD₃OD) δ6.69 (d, J=9.2 Hz, 1H), 4.10 (s, 1H), 3.60 (t, J=6.6 Hz, 2H), 3.47-3.30 (m, 2H), 3.12-2.89 (m, 9H), 2.58 (t, J=14.6 Hz, 1H), 2.22-2.18 (m, 1H), 2.02-1.93 (m, 4H), 1.63-1.54 (m, 1H); MS (ESI) m/z 530.20 (M+H).

S18-8-3:

¹H NMR (400 MHz, CDCl₃) δ 7.46-7.44 (m, 2H), 7.38-7.32 (m, 3H), 6.43 (d, J=9.8 Hz, 1H), 5.33 (s, 2H), 4.20 (br s, 1H), 3.81-3.80 (m, 2H), 3.30-3.29 (m, 2H), 3.09-3.06 (m, 2H), 2.85-2.82 (m, 16H), 2.71-2.68 (m, 1H), 2.48-2.44 (m, 1H), 2.14-2.10 (m, 1H), 1.34-1.28 (m, 1H); MS (ESI) m/z 635.27 (M+H).

S18-9-3:

¹H NMR (400 MHz, CD₃OD) δ 6.68 (d, J=9.6 Hz, 1H), 4.10 (s, 1H), 3.77 (t, J=6.0 Hz, 2H), 3.40 (t, J=6.0 Hz, 2H), 3.10-2.88 (m, 19H), 2.58 (t, J=14.2 Hz, 1H), 2.21-2.18 (m, 1H), 1.63-1.54 (m, 1H); MS (ESI) m/z 547.23 (M+H).

S18-8-4:

¹H NMR (400 MHz, CDCl₃) δ 7.49-7.47 (m, 2H), 7.41-7.35 (m, 3H), 6.61 (d, J=9.6 Hz, 1H), 5.41, 5.37 (Abq, J=11.9 Hz, 2H), 4.80 (d, J=0.92 Hz, 1H), 3.55-3.52 (m, 4H), 3.40-3.36 (m, 1H), 3.36 (s, 3H), 3.15 (s, 6H), 3.08-2.99 (m, 1H), 2.81 (dd, J=4.6, 15.6 Hz, 1H), 2.53 (t, J=14.6 Hz, 1H), 2.12-2.09 (m, 1H), 1.41-1.32 (m, 1H); MS (ESI) m/z 622.27 (M+H).

S18-9-4:

¹H NMR (400 MHz, CD₃OD) δ 6.63 (d, J=9.6 Hz, 1H), 4.07 (s, 1H), 3.56-3.52 (m, 4H), 3.37 (s, 3H), 3.10-2.93 (m, 8H), 2.90-2.86 (m, 1H), 2.57 (t, J=14.2 Hz, 1H), 2.19-2.16 (m, 1H), 1.63-1.54 (m, 1H); MS (ESI) m/z 534.19 (M+H).

Example 19. Synthesis of Compounds Via Scheme 19

The following compounds were prepared according to Scheme 19.

NBS (0.79 g, 4.44 mmol, 1.2 equiv) and AIBN (61 mg, 0.37 mmol, 0.10 equiv) were added to a suspension of compound S18-4 (1.28 g, 3.70 mmol, 1.0 equiv) in carbon tetrachloride (37 mL) at rt. The resulting mixture was stirred at 80° C. for 16 hrs, cooled to rt, and diluted with dichloromethane (40 mL). The resulting mixture was washed with saturated aqueous NaHCO₃ and brine (1:1, 40 mL). The aqueous layer was further extracted with dichloromethane (2×15 mL). The combined organic phase was dried over anhydrous magnesium sulfate, filtered, and concentrated. The residue was purified by flash column chromatography (0-5% EtOAc/hexanes) to afford the desired product S19-1 as a white solid (1.04 g, 66%): ¹H NMR (400 MHz, CDCl₃) δ 7.45-7.41 (m, 2H), 7.30-7.27 (m, 3H), 7.10 (dd, J=2.8, 8.7 Hz, 1H), 7.01 (dd, J=2.8, 8.7 Hz, 1H), 4.70 (s, 2H), 1.44 (s, 9H); MS (ESI) m/z 423.16, 425.13 (M−H).

A solution of phenyllithium in di-n-butyl ether (1.22 mL, 1.8 M, 2.20 mmol, 3.0 equiv) was added dropwise via a syringe to a solution of S19-1 (0.94 g, 2.20 mmol, 3.0 equiv) and enone S1-9 (0.35 g, 0.73 mmol, 1.0 equiv) in THF (37 mL) at −100° C. The orange reaction mixture was allowed to warm to −78° C. over 10 min. Then a solution of LHMDS in hexanes (0.73 mL, 1.0 M/THF, 0.73 mmol, 1.0 equiv) was added dropwise at −78° C. The reaction mixture was allowed to warm slowly to −10° C. over 50 min, and then partitioned between aqueous potassium phosphate buffer solution (pH 7, 0.2 M, 50 mL) and EtOAc (120 mL). The phases were separated and the organic phase was dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 3.0 mL (CH₃CN); gradient: 80→100% B in A over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 4.3-6.8 min, were collected and concentrated to yield compound S19-2 (0.49 g, 90%): ¹H NMR (400 MHz, CDCl₃) δ 15.69 (s, 1H), 7.49-7.47 (m, 2H), 7.38-7.32 (m, 3H), 6.84 (d, J=7.9 Hz, 1H), 6.80 (d, J=8.5 Hz, 1H), 5.34 (s, 2H), 3.92 (d, J=10.4 Hz, 1H), 3.10-3.03 (m, 1H), 2.94-2.78 (m, 2H), 2.52-2.40 (m, 8H), 2.07 (d, J=14.6 Hz, 1H), 1.98 (s, 9H), 1.53 (s, 9H), 0.26 (s, 3H), 0.11 (s, 3H); MS (ESI) m/z 735.35 (M+H).

Aqueous HF (48-50%, 0.5 mL) was added to a solution of compound S19-2 (0.49 g, 0.66 mmol) in acetonitrile (1.0 mL) in a polypropylene reaction vessel at 23° C. The mixture was stirred vigorously at 23° C. overnight, poured into aqueous K₂HPO₄ (6 g dissolved in 40 mL water), and extracted with EtOAc (100 mL, then 2×20 mL). The combined organic extracts were dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was used directly in the next step.

Pd—C (10 wt %, 10 mg) was added in one portion into a solution of the above product in a mixture of MeOH (2 mL) and dioxane (0.5 mL) at 23° C. The reaction vessel was sealed and purged with hydrogen by briefly evacuating the flask followed by flushing with hydrogen gas (1 atm). The reaction was stirred at 23° C. for 30 min. The reaction mixture was then filtered through a small Celite pad. The filtrate was concentrated. The residue was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 15→60% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product were collected and freeze-dried to yield compound S19-3 (0.22 g, 66% over 2 steps): ¹H NMR (400 MHz, CD₃OD) δ 6.65-6.52 (m, 2H), 4.08 (s, 1H), 3.06-2.93 (m, 8H), 2.86 (dd, J=4.1, 15.1 Hz, 1H), 2.54 (t, J=14.6 Hz, 1H), 2.21-2.16 (m, 1H), 1.62-1.53 (m, 1H); MS (ESI) m/z 433.19 (M+H).

Compound S19-3 (0.15 g, 0.31 mmol, 1.0 equiv) was dissolved in concentrated sulfuric acid (1 mL) at 0° C., forming a red solution. NaNO₃ (39 mg, 0.46 mmol, 1.5 equiv) was then added in one portion at 0° C. The resulting orange reaction mixture was stirred at 0° C. for 30 min, and was then added dropwise to vigorously stirring diethyl ether (100 mL). The precipitates were collected onto a small Celite pad, washed with more ether, and eluted with MeOH (20 mL). The MeOH solution was concentrated. The residue was used directly in the next step.

Pd—C (10 wt %, 50 mg) was added in one portion into a solution of the above products in MeOH (5 mL) at 23° C. The reaction vessel was sealed and purged with hydrogen by briefly evacuating the flask followed by flushing with hydrogen gas (1 atm). The mixture was stirred at 23° C. for 1 h. The reaction mixture was then filtered through a small Celite pad. The filtrate was concentrated. The residue was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 5→30% B over 24 min; mass-directed fraction collection] to yield compound S19-4 and S19-5.

Compound S19-4 (18 mg): ¹H NMR (400 MHz, CD₃OD) δ 6.88 (d, J=11.9 Hz, 1H), 4.15 (s, 1H), 3.20-2.95 (m, 9H), 2.45 (t, J=15.6 Hz, 1H), 2.34-2.29 (m, 1H), 1.69-1.59 (m, 1H); MS (ESI) m/z 448.18 (M+H).

Compound S19-5 (68 mg, 72% over 2 steps): ¹H NMR (400 MHz, CD₃OD) δ 6.87 (d, J=9.2 Hz, 1H), 4.16 (s, 1H), 3.16-2.94 (m, 9H), 2.60 (t, J=14.6 Hz, 1H), 2.28-2.24 (m, 1H), 1.64-1.55 (m, 1H); MS (ESI) m/z 448.18 (M+H).

2-t-Butylaminoacetyl chloride hydrochloride (4 mg, 0.020 mmol, 2.0 equiv) was added to a solution of compound S19-4 (5 mg, 0.010 mmol, 1.0 equiv) in DMF (0.3 mL) at rt. The resulting orange reaction mixture was stirred at rt for 15 min and quenched by aqueous HCl (0.5 N, 2 mL). The resulting mixture was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 0→30% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 12.4-13.6 min, were collected and freeze-dried to yield compound S19-7-1 (4 mg, 56%): ¹H NMR (400 MHz, CD₃OD) δ 6.71 (d, J=11.4 Hz, 1H), 4.11 (s, 1H), 4.06 (s, 2H), 3.12-3.08 (m, 1H), 3.07-2.96 (m, 8H), 2.31-2.24 (m, 2H), 1.62-1.52 (m, 1H), 1.41 (s, 9H); MS (ESI) m/z 561.39 (M+H).

Compound S19-6-1 was prepared from S19-5 with the same procedure: ¹H NMR (400 MHz, CD₃OD) δ 6.70 (d, J=9.6 Hz, 1H), 4.10 (s, 1H), 4.05 (s, 2H), 3.09-2.93 (m, 8H), 2.92-2.87 (m, 1H), 2.57 (t, J=14.2 Hz, 1H), 2.29-2.19 (m, 1H), 1.64-1.54 (m, 1H), 1.41 (s, 9H); MS (ESI) m/z 561.30 (M+H).

Pd—C (10 wt %, 6 mg) and formaldehyde in water (37%, 9 μL, 0.12 mmol, 5.0 equiv) were added to a solution of compound S19-4 (12 mg, 0.024 mmol, 1.0 equiv) in MeOH (1 mL) at 23° C. The reaction vessel was sealed and purged with hydrogen by briefly evacuating the flask followed by flushing with hydrogen gas (1 atm). The resulting mixture was stirred at 23° C. for 4 hrs. The reaction mixture was then filtered through a small Celite pad. The filtrate was concentrated. The residue was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 15→60% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 4.9-6.1 min, were collected and freeze-dried to yield compound S19-7-2 (9 mg, 68%): ¹H NMR (400 MHz, CD₃OD) δ 6.86 (d, J=14.2 Hz, 1H), 4.16 (s, 1H), 3.49 (dd, J=4.1, 15.6 Hz, 1H), 3.24 (br s, 6H), 3.12-3.02 (m, 2H), 3.06 (s, 3H), 2.97 (s, 3H), 2.48 (t, J=14.6 Hz, 1H), 2.33-2.30 (m, 1H), 1.70-1.61 (m, 1H); MS (ESI) m/z 476.24 (M+H).

Benzyl N-(hydroxymethyl)carbamate (10 mg, 0.057 mmol, 1.1 equiv) was added to a solution of compound S19-3 (24 mg, 0.052 mmol, 1.0 equiv) in a mixture of TFA (0.2 mL) and methanesulfonic acid (0.1 mL) at rt. The reaction mixture was stirred at rt for 14 hrs. More benzyl N-(hydroxymethyl)carbamate (5 mg, 0.026 mmol, 0.5 equiv) was added. The reaction mixture was stirred at rt for another hour, quenched by 0.05 N HCl/water (1 mL), filtered through a cotton plug, and the cotton plug was washed with 0.05 N HCl/water. The filtrate was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm, flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 0→20% B over 22 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 12.2-15.2 min, were collected and freeze-dried to yield the aminomethylated intermediates as a mixture of regioisomers (˜3:2, 10 mg, 36%): ¹H NMR (400 MHz, CD₃OD) δ 6.73-6.71 (m, 1H), 4.19 (s, 1H), 4.15 (s, 0.4H), 4.11 (s, 0.6H), 3.27-3.22 (m, 0.4H), 3.14-2.90 (m, 9.6H), 2.58 (t, J=14.6 Hz, 0.6H), 2.45 (t, J=14.2 Hz, 0.4H), 2.34-2.31 (m, 0.4H), 2.24-2.20 (m, 0.6H), 1.64-1.54 (m, 1H); MS (ESI) m/z 462.22 (M+H).

A solution of pivalaldehyde (2 μL, 0.021 mmol, 1.1 equiv) in DMF (0.2 mL) was added to the above aminomethylated intermediates. Triethylamine (5 μL, 0.038 mmol, 2.0 equiv) was added. The reaction mixture was stirred at rt for 50 min. Na(OAc)₃BH (8 mg, 0.038 mmol, 2.0 equiv) was added. After 35 min, more pivalaldehyde (0.5 μL, 0.0050 mmol, 0.25 equiv) was added. The reaction mixture was stirred at rt for another 40 min, diluted with 0.05 N HCl/water, and purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 0→20% B over 20 min, and then kept at 20% B for 5 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 21.0-21.5 min, were collected and freeze-dried to yield product S19-8-1 (1 mg, 10%): ¹H NMR (400 MHz, CD₃OD) δ 6.77 (d, J=10.1 Hz, 1H), 4.34 (s, 2H), 4.09 (s, 1H), 3.04 (s, 2H), 2.98-2.92 (m, 9H), 2.62 (t, J=14.6 Hz, 1H), 2.23-2.17 (m, 1H), 1.66-1.56 (m, 1H), 1.09 (s, 9H); MS (ESI) m/z 532.36 (M+H).

Example 20. Synthesis of Compounds Via Scheme 20

The following compounds were prepared according to Scheme 20.

Crude S18-2 (9.4 g, ˜51 mmol, 1.0 equiv) was dissolved in TFA (50 mL) and cooled to 0° C. NBS (9.54 g, 53.60 mmol, 1.05 equiv) was added in one portion. The reaction mixture was stirred at 0° C. for 1 h. Volatiles were evaporated, and the mixture was poured onto ice-water (300 mL). The resulting mixture was extracted with EtOAc (300 mL). The combined organic phases were washed with brine (100 mL), dried over anhydrous magnesium sulfate, filtered, concentrated, and further dried under high vacuum to give crude S20-1, which was used in the next reaction without further purification.

The above crude product S20-1 was dissolved in dichloromethane (100 mL). Oxalyl chloride (5.78 mL, 66.30 mmol, 1.3 equiv) was added at rt (bubbling vigorously). Additional amounts of oxalyl chloride [3×(5.78 mL, 66.30 mmol, 1.3 equiv)] were added, followed by a few drops of DMF. The mixture was stirred at rt for 1 h. Volatiles were evaporated, and the residue was further dried under high vacuum to afford the crude acid chloride. The crude acid chloride was redissolved in dichloromethane (100 mL). Phenol (5.76 g, 61.20 mmol, 1.2 equiv), triethylamine (21.30 mL, 153.00 mmol, 3.0 equiv), and DMAP (catalytic amount) were added. The reaction mixture was stirred overnight at rt. Volatiles were evaporated. The residue was dissolved in EtOAc (300 mL) and water (100 mL). The organic layer was separated and washed with 1 N aqueous HCl (70 mL), brine (50 mL), 1 N aqueous NaOH (70 mL) and brine (50 mL), dried over anhydrous magnesium sulfate, filtered, and concentrated. The residue was purified by hydrolysis of the byproducts as following.

The above crude product was dissolved in 2-methyltetrahydrofuran (25 mL). Water (25 mL) was added, followed by 50 wt % aqueous NaOH (˜19N, 2.70 mL). The reaction mixture was stirred vigorously at 75° C. for 3 hrs (monitored by LC-MS). The reaction mixture was cooled to rt diluted with EtOAc (200 mL). The organic phase was washed with 2 N aqueous NaOH (2×45 mL) and brine (50 mL), dried over anhydrous magnesium sulfate, filtered, and concentrated to afford the desired product S20-2 (10.00 g): ¹H NMR (400 MHz, CDCl₃) δ 7.46-7.42 (m, 2H), 7.31-7.29 (m, 1H), 7.26-7.22 (m, 2H), 6.67 (d, J=10.4 Hz, 1H), 3.88 (s, 3H), 2.52 (s, 3H); MS (ESI) m/z 337.15, 339.12 (M−H).

A solution of BBr₃ in dichloromethane (29.48 mL, 1.0 M, 29.48 mmol, 1.0 equiv) was added slowly to a solution of the above compound S20-2 in dichloromethane (100 mL) at −78° C. The resulting red solution was allowed to warm to 0° C. in 25 min and kept at that temperature for 10 min (monitored by LC-MS or TLC (product is slightly less polar)). The reaction mixture was poured into saturated NaHCO₃ solution (100 mL), stirred at rt for 5 min, and concentrated under reduced pressure. The residue was extracted with EtOAc (180 mL, then 30 mL). The organic extracts were combined and dried over anhydrous magnesium sulfate, filtered, and concentrated to afford the crude phenol, which was used directly in the next reaction: ¹H NMR (400 MHz, CDCl₃) δ 11.01 (d, J=1.2 Hz, 1H), 7.48-7.44 (m, 2H), 7.34-7.30 (m, 1H), 7.19-7.17 (m, 2H), 6.72 (d, J=9.2 Hz, 1H), 2.85 (s, 3H); MS (ESI) m/z 323, 325 (M−H).

Benzylbromide (3.67 mL, 30.95 mmol, 1.05 equiv) and Cs₂CO₃ powder (11.50 g, 35.38 mmol, 1.2 equiv) were added to a solution of the above crude phenol (10.00 g, 29.48 mmol, 1.0 equiv) in acetone (60 mL). The mixture was stirred at rt overnight. Solvents were evaporated and the residue was dissolved in a mixture of EtOAc (250 mL), water and brine (1:1, 150 mL). The organic layer was separated, and the aqueous layer was extracted with EtOAc (50 mL). The combined organic phase was dried over anhydrous magnesium sulfate, filtered, and concentrated. The residue was purified by flash column chromatography (1% EtOAc/hexanes), followed by re-crystallization from EtOAc/hexanes to afford the desired product S20-3 as a white solid (6.30 g, 24% five steps): ¹H NMR (400 MHz, CDCl₃) δ 7.42-7.33 (m, 7H), 7.26-7.22 (m, 1H), 7.08-7.05 (m, 2H), 6.70 (d, J=9.8 Hz, 1H), 5.12 (s, 2H), 2.51 (s, 3H); MS (ESI) m/z 413.27, 415.22 (M−H).

To a solution of S20-3 (1.33 g, 3.19 mmol, 1.0 equiv) and TMEDA (0.96 mL, 6.39 mmol, 2.0 equiv) in THF (50 mL) was added dropwise a solution of LDA (5.32 mL, 1.2 M/THF/heptane/ethylbenzene, 6.39 mmol, 2.0 equiv) at −78° C. The resulting red solution was stirred at that temperature for 15 min, and DMF (0.74 mL, 9.58 mmol, 3.0 equiv) was added. The reaction mixture was stirred at −78° C. for 1 h (monitored by LC-MS and TLC). Saturated aqueous NH₄Cl (10 mL) was added at −78° C. dropwise. The resulting mixture was warmed up to rt, diluted with water (50 mL) and extracted with EtOAc (200 mL). The organic phase was dried over anhydrous magnesium sulfate, filtered, and concentrated. The residue was purified by flash column chromatography (3-6% EtOAc/hexanes) to afford the desired compound S20-4 as an off-white solid (0.53 g): ¹H NMR (400 MHz, CDCl₃) δ 10.27 (s, 1H), 7.45-7.42 (m, 2H), 7.38-7.34 (m, 5H), 7.27-7.24 (m, 1H), 7.03-7.01 (m, 2H), 5.12 (s, 2H), 2.60 (s, 3H); MS (ESI) m/z 441.20, 443.25 (M−H).

To a solution of S20-4 (0.53 g, 1.20 mmol, 1.0 equiv) in EtOH (3 mL) and dichloromethane (2 mL) was added NaBH₄ (45 mg, 1.20 mmol, 1.0 equiv) at rt in one portion (exothermic). After 1 min, TLC showed the reaction was complete. The reaction mixture was then cooled to 0° C., and 1 N aqueous HCl solution (1 mL) was added dropwise (gas evolution). Aqueous saturated NaHCO₃ was added to neutralize the aqueous layer to pH 7. The resulting mixture was extracted with dichloromethane (50 mL). The organic phase was dried over anhydrous magnesium sulfate, filtered, and concentrated. The residue was purified by flash column chromatography (10% EtOAc/hexanes) to afford the desired compound S20-5 as a white solid (0.62 g, 43% over two steps): ¹H NMR (400 MHz, CDCl₃) δ 7.43-7.35 (m, 7H), 7.28-7.24 (m, 1H), 7.09-7.08 (m, 2H), 5.14 (s, 2H), 4.73 (dd, J=1.8, 6.7 Hz, 2H), 2.55 (s, 3H), 1.92 (t, J=6.7 Hz, 1H); MS (ESI) m/z 443.10, 445.09 (M−H).

A solution of n-butyllithium in hexanes (1.36 mL, 1.6 M, 2.18 mmol, 2.2 equiv) was added to a solution of diisopropylamine (0.31 mL, 2.18 mmol, 2.2 equiv) and TMEDA (0.33 mL, 2.18 mmol, 2.2 equiv) in THF (20 mL) at −78° C. After stirring at −78° C. for 30 min, a solution of compound S20-5 (0.44 g, 0.99 mmol, 1.0 equiv) in THF (4 mL) was added dropwise via a cannula. The resulting red reaction mixture was then stirred at −78° C. for 10 min, and cooled to −100° C. A solution of enone S1-9 (0.38 g, 0.79 mmol, 0.8 equiv) in THF (4 mL) was added to the reaction mixture via a cannula. The resulting reaction mixture was allowed to warm to −30° C. over 1.5 hrs, quenched by saturated aqueous NH₄Cl (40 mL), and extracted with EtOAc (100 mL). The organic phase were dried (sodium sulfate), filtered and concentrated. The residue was purified by a preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 3.0 mL (CH₃CN); gradient: 80→100% B in A over 10 min; mass-directed fraction collection]. Fractions containing the desired product were collected and concentrated to yield compound S20-6 (0.36 g, 54%): ¹H NMR (400 MHz, CDCl₃) δ 15.79 (br s, 1H), 7.51-7.48 (m, 2H), 7.40-7.33 (m, 8H), 5.36 (s, 2H), 5.13 (d, J=11.0 Hz, 1H), 4.18 (d, J=10.4 Hz, 1H), 4.64 (dd, J=1.2, 12.2 Hz, 1H), 4.53 (dd, J=1.2, 12.2 Hz, 1H), 3.93 (d, J=10.4 Hz, 1H), 3.40 (dd, J=10.7, 16.5 Hz, 1H), 3.06-2.99 (m, 1H), 2.61-2.57 (m, 1H), 2.54-2.47 (m, 2H), 2.50 (s, 6H), 2.19 (d, J=14.6 Hz, 1H), 1.86 (br s, 1H), 0.83 (s, 9H), 0.29 (s, 3H), 0.14 (s, 3H); MS (ESI) m/z 833.41, 835.38 (M+H).

Trifluoroacetic anhydride (0.17 mL, 1.34 mmol, 2.5 equiv) was added dropwise to a solution of compound S20-6 (0.45 g, 0.54 mmol, 1.0 equiv) and DMSO (0.19 mL, 2.68 mmol, 5.0 equiv) in THF (25 mL) at −20° C. The resulting orange reaction mixture was stirred at −20° C. for 25 min. triethylamine (0.37 mL, 2.68 mmol, 5.0 equiv) was added. After stirring for 25 min, saturated aqueous NaHCO₃ (20 mL) was added at −20° C. The resulting mixture was extracted with EtOAc (80 mL). The organic phase were dried (sodium sulfate), filtered and concentrated. The residue was purified by a preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 3.0 mL (CH₃CN); gradient: 80→100% B in A over 10 min; mass-directed fraction collection]. Fractions containing the desired product were collected and concentrated to yield compound S20-7 (0.30 g, 67%): ¹H NMR (400 MHz, CDCl₃) δ 15.69 (s, 1H), 10.12 (d, J=1.2 Hz, 1H), 7.51-7.49 (m, 2H), 7.40-7.32 (m, 8H), 5.37 (s, 2H), 5.14 (d, J=11.0 Hz, 1H), 4.93 (d, J=10.4 Hz, 1H), 3.91 (d, J=11.0 Hz, 1H), 3.48-3.44 (m, 1H), 3.07-3.02 (m, 1H), 3.61-3.46 (m, 3H), 2.51 (s, 6H), 2.20 (d, J=14.7 Hz, 1H), 1.86 (br s, 1H), 0.83 (s, 9H), 0.29 (s, 3H), 0.15 (s, 3H); MS (ESI) m/z 831.53, 833.54 (M+H).

Neopentylamine (46 μL, 0.42 mmol, 2.0 equiv), acetic acid (36 μL, 0.63 mmol, 3.0 equiv) and sodium triacetoxyborohydride (67 mg, 0.32 mmol, 1.5 equiv) were added sequentially to a solution of compound S20-7 (0.18 g, 0.21 mmol, 1.0 equiv) in 1,2-dichloroethane (5 mL) at 23° C. After stirring for 1 h, the reaction mixture was quenched by the addition of saturated aqueous sodium bicarbonate and pH 7 phosphate buffer (1:1, 30 mL) and extracted with dichloromethane (50 mL, then 10 mL). The combined organic extracts were dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was used directly in the next reaction: ¹H NMR (400 MHz, CDCl₃) δ 7.50-7.48 (m, 2H), 7.39-7.31 (m, 8H), 5.36 (s, 2H), 5.08 (d, J=10.4 Hz, 1H), 4.84 (d, J=10.4 Hz, 1H), 3.94 (d, J=11.0 Hz, 1H), 3.83, 3.77 (ABq, J=12.2 Hz, 2H), 3.43-3.38 (m, 1H), 3.04-2.99 (m, 1H), 2.60-2.44 (m, 3H), 2.50 (s, 6H), 2.29-2.16 (m, 3H), 0.82 (s, 9H), 0.28 (s, 3H), 0.14 (s, 3H); MS (ESI) m/z 902.51, 904.51 (M+H).

Aqueous HF (48-50%, 0.6 mL) was added to a solution of the above crude product S20-8-1 in acetonitrile (1.2 mL) in a polypropylene reaction vessel at 23° C. The resulting mixture was stirred vigorously at 23° C. overnight and poured into aqueous K₂HPO₄ (7.2 g dissolved in 30 mL water). The resulting mixture was extracted with EtOAc (50 mL, then 20 mL). The combined organic extracts were dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was dissolved in MeOH containing HCl/MeOH (0.5 N, 84 μL, 0.42 mmol, 2.0 equiv), concentrated, and dried under high vacuum. The residue was used directly in the next step without further purification.

The above crude product was dissolved in MeOH (5 mL). Pd—C (10 wt %, 58 mg) was added in one portion at 23° C. The reaction vessel was sealed and purged with hydrogen by briefly evacuating the flask followed by flushing with hydrogen gas (1 atm). After stirring at 23° C. for 4.5 hrs, more Pd—C (10 wt %, 10 mg) was added. The resulting mixture was stirred for 1 h 20 min and filtered through a small Celite pad. The filtrate was concentrated. The residue was purified by a preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 20→80% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 6.4-7.2 min, were collected and freeze-dried to yield compound S19-8-1 (59 mg, 46% for 3 steps).

The following compounds were prepared similarly to S19-8-1 and S20-8-1.

S20-8-2:

¹H NMR (400 MHz, CDCl₃) δ 7.50-7.48 (m, 2H), 7.39-7.32 (m, 8H), 5.36 (s, 2H), 5.11 (d, J=10.4 Hz, 1H), 4.77 (d, J=11.0 Hz, 1H), 3.93 (d, J=11.0 Hz, 1H), 3.79, 3.68 (ABq, J=12.8 Hz, 2H), 3.39 (dd, J=10.7, 16.5 Hz, 1H), 3.04-2.98 (m, 1H), 2.70-2.67 (m, 1H), 2.60-2.56 (m, 1H), 2.53-2.44 (m, 2H), 2.50 (s, 6H), 2.18 (d, J=14.0 Hz, 1H), 0.99 (d, J=6.1 Hz, 6H), 0.82 (s, 9H), 0.28 (s, 3H), 0.13 (s, 3H); MS (ESI) m/z 874.36, 876.39 (M+H).

S20-8-3:

¹H NMR (400 MHz, CDCl₃) δ 7.50-7.48 (m, 2H), 7.39-7.32 (m, 8H), 5.36 (s, 2H), 5.12 (d, J=11.0 Hz, 1H), 4.77 (d, J=10.4 Hz, 1H), 3.94 (d, J=10.4 Hz, 1H), 3.70, 3.60 (ABq, J=11.6 Hz, 2H), 3.38 (dd, J=4.3, 15.9 Hz, 1H), 3.01-2.94 (m, 1H), 2.59-2.45 (m, 3H), 2.50 (s, 6H), 2.17 (d, J=14.6 Hz, 1H), 0.03 (s, 9H), 0.82 (s, 9H), 0.28 (s, 3H), 0.12 (s, 3H); MS (ESI) m/z 888.40, 890.40 (M+H).

S20-10-4:

¹H NMR (400 MHz, CD3OD) δ 66.80 (d, J=10.1 Hz, 1H), 4.54 (dd, J=3.7, 13.3 Hz, 1H), 4.41 (d, J=13.3 Hz, 1H), 4.10 (s, 1H), 3.37-3.32 (m, 1H), 3.19-2.91 (m, 13H), 2.62 (t, J=14.6 Hz, 1H), 2.24-2.19 (m, 1H), 1.65-1.56 (m, 1H), 1.15 (d, J=3.2 Hz, 9H); MS (ESI) m/z 546.35 (M+H).

S20-10-2:

¹H NMR (400 MHz, CD3OD) δ 6.76 (d, J=9.6 Hz, 1H), 4.29 (s, 2H), 4.10 (s, 1H), 3.16-2.89 (m, 11H), 2.61 (t, J=14.4 Hz, 1H), 2.24-2.19 (m, 1H), 2.09 (hept, J=6.4 Hz, 1H), 1.66-1.56 (m, 1H), 1.06 (d, J=6.4 Hz, 6H); MS (ESI) m/z 518.35 (M+H).

S20-10-5:

¹H NMR (400 MHz, CD3OD) δ 6.76 (d, J=9.6 Hz, 1H), 4.28 (s, 2H), 4.11 (s, 1H), 3.15-2.92 (m, 11H), 2.61 (t, J=14.6 Hz, 1H), 2.24-2.20 (m, 1H), 1.72-1.56 (m, 4H), 0.98 (d, J=6.4 Hz, 6H); MS (ESI) m/z: 532.30 (M+H).

S20-10-6:

¹H NMR (400 MHz, CD3OD) δ 6.76 (d, J=10.1 Hz, 1H), 4.29 (s, 2H), 4.10 (s, 1H), 3.15-2.92 (m, 11H), 2.61 (t, J=14.6 Hz, 1H), 2.24-2.19 (m, 1H), 1.67-1.56 (m, 3H), 0.98 (s, 9H); MS (ESI) m/z 546.37 (M+H).

S20-10-7:

¹H NMR (400 MHz, CD3OD) δ 6.75 (d, J=10.1 Hz, 1H), 4.26 (s, 2H), 4.09 (s, 1H), 3.53-3.47 (m, 1H), 3.12-2.91 (m, 9H), 2.60 (t, J=14.6 Hz, 1H), 2.22-2.18 (m, 1H), 1.65-1.56 (m, 1H), 1.41 (d, J=6.4 Hz, 6H); MS (ESI) m/z 504.39 (M+H).

S20-10-8:

¹H NMR (400 MHz, CD3OD) δ 6.75 (d, J=9.6 Hz, 1H), 4.26 (s, 2H), 4.10 (s, 1H), 3.68-3.63 (m, 1H), 3.16-2.88 (m, 9H), 2.59 (t, J=14.6 Hz, 1H), 2.23-2.14 (m, 3H), 1.87-1.68 (m, 6H), 1.64-1.55 (m, 1H); MS (ESI) m/z 530.36 (M+H).

S20-10-3:

¹H NMR (400 MHz, CD3OD) δ 6.75 (d, J=9.6 Hz, 1H), 4.23 (s, 2H), 4.10 (s, 1H), 3.11-2.92 (m, 9H), 2.60 (t, J=14.2 Hz, 1H), 2.22-2.19 (m, 1H), 1.64-1.55 (m, 1H), 1.48 (s, 9H); MS (ESI) m/z 518.42 (M+H).

Isobutylamine (13 μL, 0.12 mmol, 5.0 equiv), acetic acid (7 μL, 0.12 mmol, 5.0 equiv) and sodium triacetoxyborohydride (11 mg, 0.050 mmol, 2.0 equiv) were added sequentially to a solution of compound S20-7 (21 mg, 0.025 mmol, 1.0 equiv) in 1,2-dichloroethane (0.5 mL) at 23° C. After stirring overnight, the reaction mixture was quenched by the addition of saturated aqueous sodium bicarbonate and pH 7 phosphate buffer (1:1, 20 mL) and extracted with dichloromethane (3×15 mL). The combined organic extracts were dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was purified by a preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H2O with 0.1% HCO2H; Solvent B: CH3CN with 0.1% HCO2H; injection volume: 3.0 mL (CH3CN); gradient: 20→100% B in A over 10 min; mass-directed fraction collection]. Fractions containing the desired product were collected and concentrated to yield compound S20-8-9 (5 mg, 19%): ¹H NMR (400 MHz, CDCl3) δ 16.03 (br s, 1H), 7.49-7.48 (m, 2H), 7.38-7.30 (m, 8H), 5.35 (s, 2H), 5.00 (d, J=11.0 Hz, 1H), 4.68 (d, J=11.0 Hz, 1H), 3.98 (d, J=10.4 Hz, 1H), 3.70-3.61 (m, 2H), 3.38 (dd, J=4.9, 16.5 Hz, 1H), 3.23 (br s, 2H), 3.02-2.97 (m, 1H), 2.57-2.44 (m, 3H), 2.50 (s, 6H), 2.26-2.23 (m, 2H), 2.15 (d, J=14.6 Hz, 1H), 1.80-1.74 (m, 1H), 1.65-1.52 (m, 1H), 0.98-0.93 (m, 6H), 0.83-0.80 (m, 15H), 0.28 (s, 3H), 0.13 (s, 3H); MS (ESI) m/z 941.50, 943.50 (M+H).

Aqueous HF (48-50%, 0.3 mL) was added to a solution of compound S20-8-9 in acetonitrile (0.8 mL) in a polypropylene reaction vessel at 23° C. The resulting mixture was stirred vigorously at 23° C. overnight and poured into aqueous K₂HPO₄ (3.6 g dissolved in 20 mL water). The resulting mixture was extracted with EtOAc (30 mL, then 10 mL). The combined organic extracts were dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was dissolved in MeOH containing HCl/MeOH (0.5 N, 28 μL, 0.014 mmol, 3.0 equiv), concentrated and dried under high vacuum. The residue was used directly in the next step without further purification.

The above crude product was dissolved in MeOH (1 mL). Pd—C (10 wt %, 2 mg) was added in one portion at 23° C. The reaction vessel was sealed and purged with hydrogen by briefly evacuating the flask followed by flushing with hydrogen gas (1 atm). After stirring at 23° C. for 30 min, more Pd—C (10 wt %, 3 mg) was added. The resulting mixture was stirred for 30 min, filtered through a small Celite pad, and the filtrate was concentrated. The residue was purified by a preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 15→50% B over 15 min, then 100% B for 5 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 18.2-19.0 min, were collected and freeze-dried to yield compound S20-20-9 (1 mg, 40% for 2 steps): ¹H NMR (400 MHz, CD3OD) δ 6.19 (s, 1H), 4.23 (s, 2H), 4.05 (s, 1H), 3.13-2.77 (m, 13H), 2.47 (t, J=14.2 Hz, 1H), 2.17-1.95 (m, 3H), 1.60-1.51 (m, 1H), 1.05 (d, J=6.4 Hz, 6H), 1.00 (d, J=6.4 Hz, 6H); MS (ESI) m/z 571.50 (M+H).

Example 21. Synthesis of Compounds Via Scheme 21

The following compounds were prepared according to Scheme 21.

To a white suspension of S21-1 (4.27 g, 25.40 mmol, 1.0 equiv), anhydrous acetone (4.20 mL, 57.20 mmol, 2.25 equiv), and DMAP (0.17 mg, 1.38 mmol, 0.05 equiv) in DME (60 mL) at 0° C. was added SOCl₂ (5.03 mL, 69.00 mmol, 2.70 equiv) dropwise. The resulting clear solution was stirred from 0° C. to rt for 2 hrs, and slowly added into saturated aqueous NaHCO₃ (300 mL). The mixture was extracted with EtOAc (100 mL×3). The combined extracts were dried over sodium sulfate and concentrated under reduced pressure to yield crude S21-2 as a yellow solid: R_(f) 0.45 (40% EtOAc/hexanes); ¹H NMR (400 MHz, CDCl₃) δ 6.38 (d, J=1.84 Hz, 1H), 6.25 (d, J=2.44 Hz, 1H), 2.61 (s, 3H), 1.67 (s, 6H).

To compound S21-2 (25.40 mmol, 1.0 equiv) and triethylamine (4.25 mL, 30.50 mmol, 1.2 equiv) in dry dichloromethane (70 mL) at 0° C. was added Tf₂O (4.49 mL, 26.69 mmol, 1.05 equiv) dropwise. The reaction was stirred at 0° C. for 1 hr, quenched by saturated aqueous ammonium chloride (250 mL), and extracted with dichloromethane (100 mL×3). The dichloromethane extracts were dried over sodium sulfate and concentrated under reduced pressure. Flash column chromatography on silica gel with 0%-5% EtOAc/hexanes yielded the desired compound S21-3 as a pale solid after standing in a refrigerator overnight (7.23 g, 84% 2 steps): R_(f) 0.33 (10% EtOAc/hexanes); ¹H NMR (400 MHz, CDCl₃) δ 6.82 (d, J=1.84 Hz, 1H), 6.77 (d, J=2.44 Hz, 1H), 2.71 (s, 3H), 1.71 (s, 6H).

To compound S21-3 (3.40 g, 10.00 mmol, 1.0 equiv), t-butylcarbamate (1.76 g, 15.00 mmol, 1.5 equiv), cesium carbonate (5.86 g, 18.00 mmol, 1.8 equiv), and Xantphos (0.69 g, 1.20 mmol, 0.12 equiv) in dry dioxane (50 mL) at room temperature was added Pd₂(dba)₃ (0.18 g, 0.20 mmol, 0.04 equiv Pd). The mixture was purged by bubbling with dry nitrogen gas for 5 min with gentle stirring. The reaction vessel was then heated under nitrogen at 80° C. for 5 hrs with rapid stirring. The resulting light-green mixture was cooled to rt, diluted with water (200 mL), and extracted with EtOAc (200 mL×1, 50 mL×2). The EtOAc extracts were dried over sodium sulfate and concentrated under reduced pressure. Flash column chromatography on silica gel with 0%-20% EtOAc/hexanes afforded the desired product S21-4 as a pale solid (quantitative): R_(f) 0.45 (20% EtOAc/hexanes); ¹H NMR (400 MHz, CDCl₃) δ 7.06 (d, J=1.80 Hz, 1H), 6.72 (d, J=1.80 Hz, 1H), 6.56 (br s, 1H), 2.61 (s, 3H), 1.66 (s, 6H), 1.50 (s, 9H).

Compound S21-4 (10.00 mmol, 1.0 equiv) was dissolved in dioxane (50 mL). Water (50 mL) and 6 N aqueous NaOH (8.30 mL, 50.00 mmol, 5.0 equiv) were added. The pale suspension was heated at 70° C. with stirring for 20 hrs. The resulting clear yellow solution was cooled to 0° C., carefully acidified with 0.5 N aqueous HCl to pH 5-6, and extracted with EtOAc (200 mL×1, 50 mL×5). The EtOAc extracts were dried over sodium sulfate and concentrated under reduced pressure to yield crude S21-5 as a pale solid (quantitative): R_(f) 0.10 (20% EtOAc/hexanes); ¹H NMR (400 MHz, CDCl₃ with 2 drops of CD₃OD) δ 6.85 (br s, 1H), 6.76 (br s, 1H), 2.51 (s, 3H), 1.48 (s, 9H).

To compound S21-5 (10.00 mmol, 1.0 equiv) in dry dichloromethane was added phenol (1.88 g, 20.00 mmol, 2.0 equiv), DMAP (1.34 g, 11.00 mmol, 1.1 equiv), and EDC (4.79 g, 25.00 mmol, 2.5 equiv). The light-yellow solution was stirred at rt for 20 hrs, diluted with EtOAc (300 mL), washed with saturated aqueous ammonium chloride (100 mL×2), saturated aqueous sodium bicarbonate (100 mL×2), and brine (100 mL×1). The EtOAc solution was dried over sodium sulfate and concentrated under reduced pressure to yield crude S21-6 as a light brown oil (quantitative).

Compound S21-6 (10.00 mmol, 1.0 equiv) was dissolved in dry DMF (25 mL). Di-t-butyl dicarbonate (6.55 g, 30.00 mmol, 3.0 equiv), DIEA (6.97 mL, 40.00 mmol, 4.0 equiv), and DMAP (61 mg, 0.50 mmol, 0.05 equiv) were added. The solution was stirred at it for 20 hrs, diluted with EtOAc (200 mL), and washed with water (250 mL×1), saturated aqueous sodium bicarbonate (100 mL×2), and brine (100 mL×1). The EtOAc solution was dried over sodium sulfate and concentrated under reduced pressure. Flash column chromatography on silica gel with 0%-15% EtOAc/hexanes yielded the desired product S21-7 as a white solid (3.48 g, 64% 4 steps): R_(f) 0.50 (20% EtOAc/hexanes); ¹H NMR (400 MHz, CDCl₃) δ 7.39-7.43 (m, 2H), 7.22-7.26 (m, 3H), 6.96 (br s, 1H), 6.93 (d, J=1.84 Hz, 1H), 2.51 (s, 3H), 1.43 (s, 18H), 1.43 (s, 9H).

To diisopropylamine (28 μL, 0.20 mmol, 2.0 equiv) in anhydrous THF (4 mL) at −78° C. was added n-BuLi (0.13 mL, 1.6 M/hexanes, 0.20 mmol, 2.0 equiv). The solution was stirred at 0° C. for 10 min and cooled to −78° C. TMEDA (33 μL, 0.22 mmol, 2.2 equiv) was added, followed by dropwise addition of compound S21-7 (0.11 g, 0.20 mmol, 2.0 equiv) in anhydrous THF (4 mL) over a period of 5 min. Additional LDA (0.17 mL, 1.2 M/heptane/THF/ethylbenzene, 0.20 mmol, 2.0 equiv) was added. The resulting deep-red solution was stirred at −78° C. for 15 min. Enone (48 mg, 0.10 mmol, 1.0 equiv) in anhydrous THF (4 mL) was added. The resulting red-brown solution was slowly warmed to −10° C. over a period of 1 hr, quenched by pH 7 phosphate buffer (40 mL), and extracted with EtOAc (20 mL×3). The EtOAc extracts were combined, dried over sodium sulfate, and concentrated under reduced pressure. Flash column chromatography on silica gel with 0%-10% EtOAc/hexanes yielded the desired product S21-8 as a yellow foam (85 mg, 91%): R_(f) 0.45 (20% EtOAc/hexanes); ¹H NMR (400 MHz, CDCl₃) δ 15.72 (s, 1H), 7.30-7.50 (m, 5H), 6.94 (d, J=1.8 Hz, 1H), 6.86 (d, J=1.8 Hz, 1H), 5.34 (s, 2H), 3.94 (d, J=10.4 Hz, 1H), 2.80-3.20 (m, 2H), 2.45-2.55 (m, 10H), 1.52 (s, 9H), 1.43 (s, 18H), 0.82 (s, 9H), 0.25 (s, 3H), 0.11 (s, 3H); MS (ESI) m/z 932.3 (M+H), calcd for C₄₉H₆₅N₃O₁₃Si 931.4.

To compound S21-8 (82 mg, 0.088 mmol) in THF (1.5 mL) was added 50% aqueous HF (0.5 mL, 48-50%). The bright yellow solution was stirred at rt overnight, diluted with aqueous K₂HPO₄ (7.5 g in 30 mL water), and extracted with EtOAc (20 mL×3). The EtOAc extracts were combined, dried over sodium sulfate, and concentrated under reduced pressure to yield crude S21-9 as a yellow solid (37 mg, 81%): MS (ESI) m/z 518.3 (M+H), calcd for C₂₈H₂₇N₃O₇ 517.2.

To compound S21-9 (37 mg, 0.071 mmol, 1.0 equiv) in MeOH (7 mL) and dioxane (1 mL) was added 0.5 N HCl/MeOH (2 mL) and 10% Pd—C (30 mg, 0.014 mmol, 0.20 equiv). The mixture was purged with hydrogen and stirred under 1 atm hydrogen atmosphere at rt for 1 h. The catalyst was filtered off with a small Celite pad, and the Celite pad was washed with MeOH (5 mL×3). The yellow MeOH solution was concentrated under reduced pressure. One half of the crude product was purified by reverse phase HPLC using similar conditions for S2-4-1 to give the desired product S21-10 as an orange solid (9 mg, 24%, bis-HCl salt): ¹H NMR (400 MHz, CD₃OD) δ 6.17 (s, 1H), 6.12 (s, 1H), 4.03 (s, 1H), 3.03 (s, 3H), 2.97 (s, 3H), 2.75-3.20 (m, 2H), 2.72 (dd, J=5.9, 14.8 Hz, 1H), 2.45 (t, J=14.3 Hz, 1H), 2.08-2.16 (m, 1H), 1.40-1.60 (m, 1H); MS (ESI) m/z 430.2 (M+H), calcd for C₂₁H₂₃N₃O₇ 429.2.

To compound S21-10 (7 mg, 0.014 mmol, 1.0 equiv) in MeOH (2 mL) was added HCl/MeOH (0.5 mL, 0.5 N) and 10% Pd—C (5 mg, 0.0020 mmol, 0.16 equiv). The mixture was purged with hydrogen and stirred under 1 atm hydrogen atmosphere at rt for 3 hrs. The catalyst was filtered off with a small Celite pad, and the Celite pad was washed with MeOH (2 mL×3). The yellow MeOH solution was concentrated under reduced pressure. HPLC purification using similar conditions for S2-4-1 yielded the desired product S21-11 as a bright yellow solid (4 mg, 47%, bis-HCl salt): ¹H NMR (400 MHz, CD₃OD) δ 4.06 (s, 1H), 3.13 (s, 6H), 3.03 (s, 3H), 2.97 (s, 3H), 2.90-3.20 (m, 2H), 2.83 (dd, J=4.9, 14.6 Hz, 1H), 2.50 (t, J=14.3 Hz, 1H), 2.12-2.20 (m, 1H), 1.49-1.60 (m, 1H); MS (ESI) m/z 458.3 (M+H), calcd for C₂₃H₂₇N₃O₇ 457.2.

Bromoacetyl bromide (5 μL, 0.057 mmol. 1.1 equiv) was added to compound S21-9 (36 mg, 0.051 mmol, 1.0 equiv) in THF (2 mL) at rt. The bright yellow solution was stirred at rt for 1 h. Pyrrolidine (0.021 mL, 0.25 mmol, 5.0 equiv) was added. The reaction was stirred at t for another hour and concentrated under reduced pressure to yield the crude intermediate: MS (ESI) m/z 743.4 (M+H), calcd for C₄₀H₅₀N₄O₈Si 742.3.

To the above crude intermediate (0.051 mmol) in THF (1.5 mL) was added 50% aqueous HF (0.5 mL, 48-50%). The bright yellow solution was stirred at rt for 4 hrs, diluted with aqueous K₂HPO₄ (7.5 g in 30 mL water), and extracted with EtOAc (30 mL×3). The EtOAc extracts were combined, dried over sodium sulfate, and concentrated under reduced pressure to yield the crude desilylated product as a yellow solid: MS (ESI) m/z 629.3 (M+H), calcd for C₃₄H₃₆N₄O₈ 628.2.

To the above crude desilylated product (0.051 mmol, 1.0 equiv) in MeOH (4 mL) was added HCl (1 mL, 0.5 N/MeOH) and 10% Pd—C (22 mg, 0.010 mmol, 0.2 equiv). The mixture was purged with hydrogen and stirred under 1 atm hydrogen atmosphere at rt for 1 h. The catalyst was filtered off with a small Celite pad, and the Celite pad was washed with MeOH (5 mL×3). The yellow MeOH solution was concentrated under reduced pressure. HPLC purification using similar conditions for S2-4-1 yielded the desired product S21-12-1 as a bright yellow solid (17 mg, 55% 3 steps, bis-HCl salt): ¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 6.96 (s, 1H), 4.29 (s, 2H), 4.09 (s, 1H), 3.75-3.85 (m, 2H), 3.15-3.28 (m, 2H), 3.05 (s, 3H), 2.98 (s, 3H), 2.90-3.20 (m, 2H), 2.83 (dd, J=5.2, 15.5 Hz, 1H), 2.54 (t, J=14.3 Hz, 1H), 2.00-2.22 (m, 5H), 1.50-1.65 (m, 1H); MS (ESI) m/z 541.3 (M+H), calcd for C₂₇H₃₂N₄O₈ 540.2.

Acryloyl chloride (9 μL, 0.11 mmol, 2.0 equiv) was added to compound S21-9 (36 mg, 0.051 mmol, 1.0 equiv) in THF (2 mL) at rt. The yellow solution was stirred at rt overnight. Pyrrolidine (0.021 mL, 0.25 mmol, 5.0 equiv) was added. The reaction was stirred at rt for another hour and concentrated under reduced pressure to yield the crude intermediate: MS (ESI) m/z 757.4 (M+H), calcd for C₄₁H₅₂N₄O₈Si 756.4.

The crude intermediate was treated with aqueous HF followed by hydrogenation under similar conditions to that in the preparation of compound S21-12-1 to yield the desired product S21-12-2 as a bright-yellow solid (17 mg, 53% 3 steps, bis-HCl salt): ¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 6.95 (s, 1H), 4.06 (s, 1H), 3.65-3.75 (m, 2H), 3.54 (t, J=8.0 Hz, 2H), 3.10-3.20 (m, 2H), 2.80-3.20 (m, 10H), 2.80 (dd, J=5.3, 18.7 Hz, 1H), 2.53 (t, J=14.0 Hz, 1H), 2.10-2.22 (m, 3H), 2.00-2.10 (m, 2H), 1.50-1.63 (m, 1H); MS (ESI) m/z 555.3 (M+H), calcd for C₂₈H₃₄N₄O₈ 554.2.

N-Methyl morpholine (0.028 mL, 0.25 mmol, 15 equiv), chloroethanesulfonyl chloride (0.027 mL, 0.25 mmol, 15 equiv), and DMAP (2 mg, 0.017 mmol, 1.0 equiv) were added to compound S21-9 (12 mg, 0.017 mmol, 1.0 equiv) in THF (2 mL) at rt. The reaction was stirred at rt for 72 hrs. Pyrrolidine (0.071 mL, 0.85 mmol, 50 equiv) was added. The reaction was stirred at rt for 30 min. Aqueous HF (2 mL, 48-50%) was added. The reaction was stirred at rt overnight. The yellow solution was diluted with aqueous K₂HPO₄ (20 g in 80 mL water) and extracted with EtOAc (30 mL×3). The EtOAc extracts were combined, dried over sodium sulfate, and concentrated under reduced pressure to yield the crude desilylated sulfonamide: MS (ESI) m/z 679.3 (M+H), calcd for C₄₃H₃₈N₄O₉S 678.2.

The above crude product (0.017 mmol, 1.0 equiv) was dissolved in MeOH (3 mL) and dioxane (1 mL). HCl/MeOH (1 mL, 0.5 N) and 10% Pd—C were added. The mixture was purged with hydrogen and stirred under 1 atm hydrogen atmosphere at rt for 3 hrs. The catalyst was filtered off with a small Celite pad, and the Celite pad was washed with MeOH (2 mL×3). The MeOH filtrate was concentrated under reduced pressure. HPLC purification using similar conditions for S2-4-1 yielded the desired product S21-12-3 as a bright-yellow solid (3 mg, 27% 3 steps, bis-HCl salt): ¹H NMR (400 MHz, CD₃OD) δ 6.03 (s, 1H), 5.92 (s, 1H), 4.02 (s, 1H), 3.63 (t, J=7.0 Hz, 2H), 2.80-3.40 (m, 8H), 3.02 (s, 3H), 2.96 (s, 3H), 2.70 (dd, J=4.9, 14.7 Hz, 1H), 2.41 (t, J=13.7 Hz, 1H), 1.85-1.95 (m, 4H), 1.45-1.60 (m, 1H); MS (ESI) m/z 591.3 (M+H), calcd for C₂₇H₃₄N₄O₉S 590.2.

To a suspension of S21-9 (35 mg, 0.056 mmol, 1.0 equiv) in dichloromethane (0.22 mL) was added pyridine (14 μL, 0.17 mmol, 3.0 equiv) and methanesulfonyl chloride (5 μL, 0.061 mmol, 1.1 equiv). The heterogeneous mixture was stirred at room temperature for 21 hrs, and was concentrated under reduced pressure to remove solvent and excess pyridine. This crude mixture was then dissolved in dioxane (1.2 mL) in a plastic vial. Aqueous HF (0.3 mL, 48-50%) was then added and the reaction was stirred at room temperature for 19 hrs. The reaction mixture was poured into an aqueous solution of K₂HPO₄ (3.6 g in 30 mL) and was extracted with EtOAc (2×25 mL). The combined organic layers were dried over sodium sulfate, were filtered, and were concentrated under reduced pressure to provide an orange oil. This oil was dissolved in MeOH/dioxane (1 mL, 1:1) and Pd—C (10 mg, 10 wt %) was added. The reaction was purged with hydrogen and stirred under hydrogen (balloon) at room temperature for 22 hrs. The reaction mixture was filtered through a small Celite plug and the filtrate was concentrated under reduced pressure. The crude product was purified via preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 2.2 mL (0.05 N HCl/water); gradient: 0→70% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 8.9-9.7 min, were collected and freeze-dried to yield 3 mg of compound S21-12-4 as a yellow solid (12% for 3 steps): ¹H NMR (400 MHz, CD₃OD) δ 6.63-6.61 (m, 1H), 6.58 (br s, 1H), 4.04 (s, 1H), 3.10-2.89 (m, 8H), 2.79 (dd, J=15.3, 4.3 Hz, 1H), 2.52 (t, J=14.7 Hz, 1H), 2.19-2.09 (m, 1H), 1.63-1.48 (m, 1H); MS (ESI) m/z 508.24 (M+H).

To a suspension of S21-9 (35 mg, 0.056 mmol, 1.0 equiv) in dichloromethane (0.22 mL) was added pyridine (14 μL, 0.17 mmol, 3.0 equiv) and benzenesulfonyl chloride (8 μL, 0.061 mmol, 1.1 equiv). The heterogeneous mixture was stirred at room temperature for 21 hrs and was concentrated under reduced pressure to remove solvent and excess pyridine. This crude mixture was then dissolved in dioxane (1 mL) in a plastic vial. Aqueous HF (0.25 mL, 48-50%) was added and the reaction mixture was stirred at room temperature for 3 hrs. The reaction mixture was poured into an aqueous solution of K₂HPO₄ (3.0 g in 25 mL) and was extracted with EtOAc (2×25 mL). The combined organic layers were dried over sodium sulfate, were filtered, and were concentrated under reduced pressure to provide an orange oil. This oil was dissolved in MeOH/dioxane (1 mL, 1:1) and Pd—C (10 mg, 10 wt %) was added. The reaction was purged with hydrogen and stirred under hydrogen (balloon) at room temperature for 17 hrs. The reaction mixture was filtered through a small Celite plug and the filtrate was concentrated under reduced pressure. The crude product was purified via preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.8 mL (0.05 N HCl/water); gradient: 20→70% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 9.0-10.1 min, were collected and freeze-dried to yield 4 mg of compound S21-12-5 as a yellow solid (13% for 3 steps): ¹H NMR (400 MHz, CD₃OD) δ 7.90-7.85 (m, 2H), 7.63-7.57 (m, 1H), 7.56-7.49 (m, 2H), 6.56-6.53 (m, 1H), 6.48 (br s, 1H), 3.99 (s, 1H), 3.01-2.83 (m, 8H), 2.69 (dd, J=15.3, 4.3 Hz, 1H), 2.43 (t, J=14.6 Hz, 1H), 2.15-2.06 (m, 1H), 1.59-1.46 (m, 1H); MS (ESI) m/z 570.25 (M+H).

To a suspension of S21-9 (34 mg, 0.053 mmol, 1.0 equiv) in dichloromethane (0.22 mL) was added trimethylacetylchloride (7 μL, 0.058 mmol, 1.1 equiv). The mixture was heterogeneous after 2 hrs and the reaction was complete after 4 hrs. The reaction mixture was concentrated under reduced pressure. The residue was redissolved in dioxane (1.2 mL) in a plastic vial. Aqueous HF (0.25 mL, 48-50%) was added and the reaction mixture was stirred at room temperature for 13 hrs. The reaction mixture was poured into an aqueous solution of K₂HPO₄ (3.1 g in 25 mL) and was extracted with EtOAc (2×25 mL). The combined organic layers were dried over sodium sulfate, were filtered, and were concentrated under reduced pressure to provide an orange oil. This oil was dissolved MeOH/dioxane (1 mL, 1:1) and Pd—C (10 mg, 10 wt %) was added. The reaction was purged with hydrogen and stirred under hydrogen (balloon) at room temperature for 2 hrs. The reaction mixture was filtered through a small Celite plug and the filtrate was concentrated under reduced pressure. The crude product was purified via preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 2.2 mL (0.05 N HCl/water); gradient: 20→70% B over 15 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 8.0-9.5 min, were collected and freeze-dried to yield 15 mg of compound S21-12-6 as a yellow solid (53% for 3 steps): ¹H NMR (400 MHz, CD₃OD) δ 7.18 (d, J=1.93, 1H), 7.04 (br s, 1H), 4.06 (s, 1H), 3.11-2.88 (m, 8H), 2.76 (dd, J=15.3, 4.2 Hz, 1H), 2.51 (t, J=14.0 Hz, 1H), 2.20-2.12 (m, 1H), 1.62-1.49 (m, 1H), 1.27 (s, 9H); MS (ESI) m/z 514.32 (M+H).

Example 22. Synthesis of Compounds Via Scheme 22

The following compounds were prepared according to Scheme 22.

Compound S21-3 (1.02 g, 3.00 mmol, 1.0 equiv) was dissolved in dry 1,4-dioxane (20 mL). 4-Aminopyridine (0.42 g, 4.50 mmol, 1.5 equiv), cesium carbonate (1.76 g, 5.40 mmol, 1.8 equiv), Xantphos (0.21 g, 0.36 mmol, 0.12 equiv), and Pd₂(dba)₃ (55 mg, 0.060 mmol, 0.02 equiv) were added. The mixture was purged with dry nitrogen, heated at 80° C. with vigorous stirring for 2 hrs, cooled to rt, diluted with water (100 mL), and extracted with EtOAc (100 mL×1, 20 mL×2). The EtOAc extracts were combined, dried over sodium sulfate, and concentrated under reduced pressure. Flash column chromatography on silica gel with 50% EtOAc/hexanes, 100% EtOAc/hexanes, and 10% MeOH/dichloromethane yielded the desired product S22-1 as a pale solid (0.613 g, 72%): R_(f)=0.17 10% MeOH/dichloromethane; ¹H NMR (400 MHz, CD₃OD) δ 8.30 (d, J=6.7 Hz, 2H), 6.98 (d, J=6.7 Hz, 2H), 6.58 (s, 2H), 2.60 (s, 3H), 1.66 (s, 6H); MS (ESI) m/z 285.0 (M+H), calcd for C₁₆H₁₆N₂O₃ 284.1.

Compound S22-1 (0.55 g, 1.93 mmol, 1.0 equiv) was dissolved in dioxane (9 mL) and water (7.4 mL). Aqueous NaOH (1.61 mL, 6 N, 9.66 mmol, 5.0 equiv) was added. The suspension was heated at 70° C. with stirring overnight, cooled to rt, neutralized with 6 N aqueous HCl to pH 6-7. The precipitates were collected, washed with water (2 mL×3) and ethanol (2 mL×3), and dried under reduced pressure to yield the desired compound S22-2 as a pale solid (quantitative): ¹H NMR (400 MHz, CD₃OD) δ 8.15 (d, J=7.3 Hz, 2H), 7.15 (d, J=7.3 Hz, 2H), 6.59 (d, J=1.8 Hz, 1H), 6.53 (d, J=1.8 Hz, 1H), 2.61 (s, 3H); MS (ESI) m/z 245.0 (M+H), calcd for C₁₃H₁₂N₂O₃ 244.1.

Compound S22-2 (0.57 g, 2.34 mmol, 1.0 equiv) was dissolved in dichloromethane (50 mL). Phenol (0.44 g, 4.68 mmol, 2.0 equiv), DMAP (0.32 g, 2.58 mmol, 1.1 equiv), DIEA (1.22 mL, 7.00 mmol, 3.0 equiv), and EDC (1.12 g, 5.84 mmol, 2.5 equiv) were added. The solution was stirred at rt for 48 hrs. Most of the dichloromethane was removed under reduced pressure. The residue was re-dissolved in EtOAc (100 mL), washed with water (100 mL×2), saturated aqueous sodium bicarbonate (50 mL×2) and brine (50 mL×1), dried over sodium sulfate, and concentrated under reduced pressure to yield the crude phenyl ester as a pale foam.

The above crude phenyl ester was dissolved in dry DMF (10 mL). Di-t-butyl dicarbonate (1.66 g, 7.61 mmol, 3.0 equiv), DIEA (1.77 mL, 10.16 mmol, 4.0 equiv), and DMAP (16 mg, 0.13 mmol, 0.05 equiv) were added. The reaction was stirred at rt for 24 hrs. More di-t-butyl dicarbonate (0.60 g) and DIEA (0.60 mL) were added. Stirring was continued at rt for another 24 hrs. The deep red reaction solution was diluted with EtOAc (100 mL), washed with water (200 mL×1), saturated aqueous sodium bicarbonate (100 mL×2) and brine (100 mL×1), dried over sodium sulfate, and concentrated under reduced pressure. Flash column chromatography on silica gel with 0% to 5% MeOH/dichloromethane yielded the desired product S22-3 as a light-yellow foam (0.18 g, 13%): R_(f)=0.80 (10% MeOH/dichloromethane); ¹H NMR (400 MHz, CDCl₃) δ 8.49 (d, J=6.1 Hz, 2H), 7.40-7.45 (m, 2H), 7.20-7.30 (m, 3H), 7.15 (d, J=6.1 Hz, 2H), 6.95 (s, 1H), 6.90 (s, 1H), 2.49 (s, 3H), 1.46 (s, 9H), 1.42 (s, 9H); MS (ESI) m/z 521.3 (M+H), calcd for C₂₉H₃₂N₂O₇ 520.2.

To diisopropylamine (0.053 mL, 0.37 mmol, 2.2 equiv) in anhydrous THF (5 mL) at −78° C. was added n-BuLi (0.24 mL, 1.6 M/hexanes, 0.38 mmol, 2.2 equiv). The pale solution was stirred at 0° C. for 10 min and cooled to −78° C. TMEDA (0.062 mL, 0.41 mmol, 2.4 equiv) was added, followed by dropwise addition of a solution of compound S22-3 (0.18 g, 0.34 mmol, 2.0 equiv) in THF (4 mL) over a period of 10 min. Additional LDA (0.38 mmol, 2.2 equiv) was added. The deep red solution was stirred at −78° C. for 15 min. Enone S1-9 (82 mg, 0.017 mmol, 1.0 equiv) in THF (5 mL) was added dropwise over a period of 5 min. The red solution was slowly warmed to −10° C. over a period of 1 h, quenched by pH 7 phosphate buffer (1 M, 50 mL), and extracted with EtOAc (20 mL×3). The EtOAc extracts were combined, dried over sodium sulfate, and concentrated under reduced pressure. Flash column chromatography on silica gel with 0% to 2% MeOH/dichloromethane yielded the desired product S22-4 as a yellow oil (0.20 g, ˜80% pure): MS (ESI) m/z 909.3 (M+H), calcd for C₄₉H₆₀N₄O₁₁Si 908.4.

Compound S22-4 (0.17 mmol) in dioxane (5 mL) was added with 4 N HCl/dioxane (5 mL). The reaction was stirred at rt overnight and concentrated under reduced pressure. The residue was partitioned between aqueous sodium bicarbonate (50 mL) and EtOAc (50 mL). The aqueous layer was extracted with more EtOAc (20 mL×3). The combined organic layers were dried over sodium sulfate and concentrated under reduced pressure. Flash column chromatography on silica gel with 0% to 10% MeOH/dichloromethane yielded the desired product S22-5 as a yellow solid (62 mg, 51% 2 steps): ¹H NMR (400 MHz, CDCl₃) δ 14.06 (br s, 1H), 12.03 (s, 1H), 11.43 (s, 1H), 7.17-7.50 (m, 8H), 6.95-7.05 (m, 3H), 5.35 (s, 2H), 3.89 (d, J=9.8 Hz, 1H), 3.00-3.10 (m, 1H), 2.7-2.95 (m, 2H), 2.40-2.55 (m, 2H), 2.46 (s, 6H), 2.00-2.10 (m, 1H), 0.84 (s, 9H), 0.28 (s, 3H), 0.14 (s, 3H); MS (ESI) m/z 709.4 (M+H), calcd for C₃₉H₄₄N₄O₇Si 708.3.

Compound S22-5 (15 mg, 0.021 mmol) was treated with aqueous HF followed by hydrogenation under similar conditions to that in the preparation of compound S21-12-1 to yield the desired product S22-6 as a bright-yellow solid (6 mg, 46% 2 steps, tris-HCl salt): ¹H NMR (400 MHz, CD₃OD) δ 8.30 (d, J=7.3 Hz, 2H), 7.38 (d, J=7.3 Hz, 2H), 6.80 (s, 1H), 6.76 (s, 1H), 4.10 (s, 1H), 2.85-3.50 (m, 3H), 3.05 (s, 3H), 2.98 (s, 3H), 2.55-2.65 (m, 1H), 2.15-2.25 (m, 1H), 1.55-1.65 (m, 1H); MS (ESI) m/z 507.1 (M+H), calcd for C₂₆H₂₆N₄O₇ 506.2.

Compound S22-5 (15 mg, 0.021 mmol, 1.0 equiv) was dissolved in dichloromethane. DIEA (0.030 mL, 0.17 mmol, 8.0 equiv) and acetic anhydride (7μ, 0.069 mmol, 3.5 equiv) were added in three equal portions over a period of 4 days at rt. MeOH (1 mL) was added. The yellow solution was concentrated under reduced pressure. HPLC purification using similar conditions for S2-4-1 yielded the desired product S22-7-1 as a yellow solid (7 mg, 44%): ¹H NMR (400 MHz, CDCl₃) δ 15.82 (br s, 1H), 8.32 (br d, J=5.5 Hz, 2H), 7.40-7.50 (m, 2H), 7.30-7.40 (m, 3H), 7.05 (br d, J=5.5 Hz, 2H), 6.90 (s, 1H), 6.76 (s, 1H), 5.34 (s, 2H), 3.89 (d, J=10.4 Hz, 1H), 3.00-3.10 (m, 1H), 2.75-2.95 (m, 2H), 2.35-2.55 (m, 2H), 2.45 (s, 6H), 2.30 (s, 3H), 2.00-2.10 (m, 1H), 0.83 (s, 9H), 0.26 (s, 3H), 0.12 (s, 3H); MS (ESI) m/z 751.4 (M+H), calcd for C₄₁H₄₆N₄O₈Si 750.3.

Compound S22-7-1 (7 mg, 0.0090 mmol) was treated with aqueous HF followed by hydrogenation under similar conditions to that in the preparation of compound S21-12-1 to yield the desired product S22-8-1 as a yellow solid (3 mg, 54% 2 steps, bis-HCl salt): ¹H NMR (400 MHz, CD₃OD) δ 8.31 (d, J=7.4 Hz, 2H), 7.40 (d, J=7.3 Hz, 2H), 6.80 (s, 1H), 6.78 (s, 1H), 4.37 (s, 1H), 2.70-3.50 (m, 9H), 2.55-2.65 (m, 1H), 2.30-2.40 (m, 1H), 2.11 (s, 3H), 1.75-1.80 (m, 1H); MS (ESI) m/z 549.1 (M+H), calcd for C₂₈H₂₈N₄O₈ 548.2.

Example 23. Synthesis of Compounds Via Scheme 23

The following compounds were prepared according to Scheme 23.

To a solution of S1-4 (8.40 g, 34.10 mmol, 1.0 equiv) in acetonitrile (41 mL) in a vial was added N-chlorosuccinimide (5.05 g, 37.80 mmol, 1.1 equiv). The vial was sealed and heated to 80° C. for 2 h. The reaction mixture was concentrated under reduced pressure, then diluted with water (25 mL) and extracted with EtOAc (2×75 mL). The combined organic layers were washed sequentially with saturated aqueous sodium bicarbonate solution (2×20 mL), water (20 mL), and brine (20 mL). The combined organic layers were then dried over sodium sulfate, filtered, and concentrated under reduced pressure. The resulting crude oil was dissolved in acetone (70 mL). To this solution was added potassium carbonate (9.448 g, 68.40 mmol, 2.0 equiv) and benzyl bromide (4.89 mL, 40.90 mmol, 1.2 equiv). The reaction was stirred at room temperature for 17 hrs. The resulting slurry was filtered through Celite, concentrated under reduced pressure, and purified by flash column chromatography (Biotage 340 g column, 2% to 10% EtOAc in hexanes gradient) to provide S23-1 as a colorless oil (7.31 g, 58%): ¹H NMR (400 MHz, CDCl₃) δ 57.49-7.44 (m, 2H), 7.40-7.32 (m, 5H), 7.29-7.21 (m, 2H), 7.06-7.00 (3, 2H), 5.10 (s, 2H), 2.34 (d, J=1.8 Hz, 3H); MS (ESI) m/z 369.13 (M−H).

LDA/THF was prepared by adding n-BuLi (1.6 M, 1.31 mL, 2.10 mmol, 1.5 equiv) to diisopropylamine (0.30 mL, 2.10 mmol) in THF (5 mL) at −78° C. The reaction mixture was warmed to −20° C. and stirred for 10 min. After the LDA solution was cooled to −78° C., compound S23-1 (0.52 g, 1.40 mmol) in THF (2 mL) was added dropwise, forming an orange-red solution. After 10 min, a solution of iodine (0.76 g, 2.97 mmol, 2.1 equiv) in THF (2 mL) was added dropwise to the above reaction mixture. The reaction solution was allowed to warm to −15° C. in 1 h and quenched by saturated aqueous NH₄Cl. LC/MS indicated that the starting material was all consumed. The reaction mixture was diluted with EtOAc (100 mL), washed with a 5% sodium thiosulfate solution (2×30 mL), water and brine, dried over sodium sulfate, filtered, and concentrated under reduced pressure. The resulting crude oil was purified by flash column chromatography (Silicycle 12 g column, 1% to 5% EtOAc in hexanes gradient), to provide S23-2 (0.56 g, 80%): ¹H NMR (400 MHz, CDCl₃) δ 7.48-7.43 (m, 2H), 7.39-7.32 (m, 5H), 7.28-7.22 (m, 1H), 7.05-6.99 (m, 2H), 5.08 (s, 2H), 2.39 (d, J=2.4 Hz, 3H); MS (ESI) m/z 495.03 (M−H).

A vial charged with S23-2 (47 mg, 0.095 mmol, 1.0 equiv), tris-(dibenzylideneacetone)dipalladium(0) (4 mg, 0.0047 mmol, 0.05 equiv), Xantphos (11 mg, 0.018 mmol, 0.19 equiv), and cesium carbonate (95 mg, 0.029 mmol, 3.0 equiv) was fitted with a septum, and evacuated and back-filled with nitrogen three times. Dioxane (0.50 mL) and pyrrolidine (0.040 mL, 0.48 mmol, 5.0 equiv) were added and the mixture was degassed by bubbling nitrogen through the heterogeneous solution for two minutes. The mixture was heated at 80° C. with stirring for 2 hrs. The reaction mixture was cooled to rt, filtered through Celite, and concentrated under reduced pressure. The resulting oil was purified by flash column chromatography (Silicycle 4 g column, 1% to 5% EtOAc in hexanes gradient), to provide compound S23-3-1 (23 mg, 55%): ¹H NMR (400 MHz, CDCl₃) δ 7.52-7.46 (m, 2H), 7.40-7.30 (m, 5H), 7.27-7.22 (m, 1H), 7.08-7.03 (m, 2H), 5.10 (s, 2H), 3.50-3.41 (m, 4H), 2.34 (d, J=2.4 Hz, 3H), 2.00-1.90 (m, 4H); MS (ESI) m/z 440.15 (M+H).

To a solution of LDA (20.053 mL, 2.0 M/THF/heptane/ethylbenzene, 0.10 mmol, 2.5 equiv) in THF (1 mL) at −70° C. was added TMEDA (0.016 mL, 0.10 mmol, 2.5 equiv). A solution of compound S23-3-1 (23 mg, 0.052 mmol, 1.25 equiv) in THF (0.5 mL) was added dropwise. The reaction was stirred at −70° C. for 1 h. A solution of enone S1-9 (21 mg, 0.043 mmol, 1.0 equiv) in THF (0.5 mL) was added dropwise to the reaction mixture. The reaction was stirred from −70° C. to −20° C. over 50 minutes, quenched by saturated aqueous NH₄Cl, and extracted with EtOAc (2×30 mL). The combined organic extracts were dried over sodium sulfate and concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; gradient: 90→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 8.0-8.75 min, were collected and freeze-dried to give 10 mg of pure compound S23-4-1 (24%): ¹H NMR (400 MHz, CDCl₃) δ 16.2 (s, 1H), 7.59-7.54 (m, 2H), 7.51-7.46 (m, 2H), 7.40-7.29 (m, 6H), 5.35 (s, 2H), 4.94 (d, J=9.8 Hz, 1H), 4.89 (d, J=9.8 Hz, 1H), 3.95 (d, J=10.4 Hz, 1H), 3.75-3.65 (m, 2H), 3.59-3.50 (m, 2H), 3.23-3.15 (m, 1H), 3.00-2.88 (m, 1H), 2.55-2.30 (m, 9H), 2.14-2.07 (m, 1H), 1.99-1.83 (m, 4H), 0.81 (s, 9H), 0.26 (s, 3H), 0.11 (s, 3H); MS (ESI) m/z 828.30 (M+H).

A solution of compound S23-4-1 (10 mg, 0.012 mmol) in 1,4-dioxane (1.3 mL) was treated with HF (0.20 mL, 48-50% aqueous solution). After stirring overnight, the mixture was poured into a solution of K₂HPO₄ (2.4 g) in water (25 mL) and extracted with EtOAc (2×30 mL). The combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure.

The above crude material was dissolved in MeOH (0.3 mL), 1,4-dioxane (0.5 mL), and 0.5 N HCl/MeOH (0.2 mL). 10% Pd—C (Degussa, 5 mg) was added, and an atmosphere of hydrogen was introduced. After 17 hrs, the reaction mixture was filtered through Celite and concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: CH₃CN, gradient elution with 15→80% B over 10 min, then 80-100% over 2 min, held at 100% for 2 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 9.9-11.2 min, were collected and freeze-dried to yield 1 mg of compound S23-5-1 (27%): ¹H NMR (400 MHz, CD₃OD) δ 5.94 (d, J=7.3 Hz, 1H), 4.03 (s, 1H), 3.56-3.49 (m, 4H), 3.13-2.84 (m, 9H), 2.20-2.09 (m, 2H), 2.03-1.91 (m, 4H), 1.63-1.50 (m, 1H); MS (ESI) m/z 502.24 (M+H).

Example 24. Synthesis of Compounds Via Scheme 24

The following compounds were prepared according to Scheme 24.

BBr₃ solution in DCM (1.0 M, 27.96 mL, 27.96 mmol, 1.0 equiv) was added to a solution of the above phenyl ester S15-5 (8.98 g, 27.96 mmol, 1.0 equiv) in DCM (100 mL) at −78° C. The resulting reaction mixture was stirred at that temperature for 20 min and at 0° C. for 15 min. Saturated NaHCO₃ solution (120 mL) was added slowly. The resulting mixture was stirred at rt for 20 min and DCM was evaporated. The residue was extracted with ethyl acetate (250 mL). The organic was separated and dried over anhydrous MgSO₄. The dried solution was filtered, and the filtrate was concentrated to afford an off-white solid. The residue was purified by recrystallization from EtOAc/Hexanes to give the desired product S24-1 as a white solid (6.76 g), and the mother liquor was concentrated and purified by flash-column chromatography (2-10% ethyl acetate-hexanes) to afford additional product (973 mg) (90% yield totally).

¹H NMR (400 MHz, CDCl₃) δ 11.13 (s, 1H), 7.47-7.43 (m, 2H), 7.33-7.29 (m, 1H), 7.19-7.16 (m, 2H), 7.08 (d, J=1.8 Hz, 1H), 6.96 (d, J=1.8 Hz, 1H), 2.66 (s, 3H); MS (ESI) m/z 305.05, 307.05 (M−H).

A solution of PhI(OAc)₂ (3.77 g, 11.72 mmol, 2.1 equiv) in MeOH (20 mL) was added slowly to a solution of S24-1 (1.71 g, 5.58 mmol, 1.0 equiv) in a mixture of MeOH (30 mL) and dioxane (10 mL) at 0° C. The reaction mixture was then stirred at rt for 17 h. HOAc (6 mL) was added to the reaction mixture. Then Zn dust (1.09 g, 16.74 mmol, 3.0 equiv) was added (slightly exothermic). The resulting reaction mixture was stirred at rt for 20 min, and filtered through a pad of Celite. The cake was washed with EtOAc (100 mL) thoroughly. The filtrate was concentrated. The residue was partitioned between EtOAc (120 mL) and sat. NaHCO₃/brine solution. (The aqueous layer pH was adjusted to 7). The organic layer was separated and dried (MgSO₄). The dried solution was filtered, and the filtrate was concentrated. The residue was purified by flash-column chromatography (0-4% ethyl acetate-hexanes) to afford the desired product S24-2 (763 mg, 41%). ¹H NMR (400 MHz, CDCl₃) δ 10.70 (s, 1H), 7.47-7.43 (m, 2H), 7.33-7.30 (m, 1H), 7.20-7.17 (m, 2H), 7.16 (s, 1H), 3.75 (s, 3H), 2.67 (s, 3H); MS (ESI) m/z 335.11, 337.14 (M−H).

Di-tert-butyl dicarbonate (543 mg, 2.49 mmol, 1.1 equiv) and N,N-dimethylaminopyridine (28 mg, 0.226 mmol, 0.1 equiv) were added to a solution of phenol S24-2 (763 mg, 2.26 mmol, 1.0 equiv) in methylene chloride (20 mL). The resulting mixture was stirred for 20 min at rt (monitored by LC-MS and TLC (product is slightly more polar)), and concentrated. The residue was purified by flash-column chromatography (0-5% ethyl acetate-hexanes) to afford the Boc protection product S24-3 as a white solid (783 mg, 79%). ¹H NMR (400 MHz, CDCl₃) δ 7.45-7.41 (m, 2H), 7.38 (s, 1H), 7.30-7.26 (m, 1H), 7.24-7.22 (m, 2H), 3.81 (s, 3H), 2.47 (s, 3H), 1.43 (s, 9H); MS (ESI) m/z 435.14, 437.15 (M−H).

A solution of ^(i)PrMgCl.LiCl in THF (1.2 M, 547 μL, 0.657 mmol, 2.0 equiv) was added dropwise to a solution of compound S24-3 (143.6 mg, 0.328 mmol, 1.0 equiv) in THF (3.3 mL) at 0° C. The resulting yellow reaction mixture was then stirred at that temperature for 1 h. Then DMF (127 μL, 1.64 mmol, 5.0 equiv) was added at 0° C. The resulting mixture was stirred at 0° C. for 10 min and then at rt for 20 min. Sat. NH₄Cl and brine were added. The resulting mixture was extracted with EtOAc (50 mL). The organic layer was separated and dried (MgSO₄). The dried solution was filtered, and the filtrate was concentrated. The crude product S24-4 was used directly for the next step. ¹H NMR (400 MHz, CDCl₃) δ 10.38 (s, 1H), 7.61 (s, 1H), 7.46-7.42 (m, 2H), 7.32-7.28 (m, 1H), 7.26-7.24 (m, 2H), 3.91 (s, 3H), 2.46 (s, 3H), 1.45 (s, 9H); MS (ESI) m/z 385.24 (M−H).

Trimethyl orthoformate (180 μL, 1.64 mmol, 5.0 equiv) and p-TSA (3.1 mg, 0.016 mmol, 0.05 equiv) were added to a solution of the above crude product S24-4 in MeOH (1 mL). The resulting reaction mixture was heated at reflux for 1 h, and cooled to rt. The solvent was evaporated and the residue was diluted with EtOAc (40 mL) and sat. NaHCO₃/brine solution (1:1, 20 mL). The organic phase was separated and dried (MgSO₄). The dried solution was filtered, and the filtrate was concentrated to afford an orange oil. The crude ¹H NMR (CDCl₃) showed desired product 6 and deBoc product (˜3:1). The crude products were used directly for the next step.

The above crude material was dissolved in DCM (1 mL). Di-tert-butyl dicarbonate (26.8 mg, 0.123 mmol) and N,N-dimethylaminopyridine (1 mg, 0.08 mmol) were added. The resulting mixture was stirred for 40 min at rt and concentrated. The residue was purified by flash-column chromatography (2-10% ethyl acetate-hexanes) to afford the desired product S24-5 as a colorless oil (124.2 mg, 87% over 3 steps). ¹H NMR (400 MHz, CDCl₃) δ 7.45-7.41 (m, 2H), 7.33 (s, 1H), 7.30-7.24 (m, 3H), 5.65 (s, 1H), 3.79 (s, 3H), 3.36 (s, 6H), 2.43 (s, 3H), 1.43 (s, 9H); MS (ESI) m/z 431.29 (M−H).

nBuLi solution (2.5 M in hexanes, 138 μL, 0.345 mmol, 1.2 equiv) was added dropwise to a solution of diisopropylamine (49 μL, 0.345 mmol, 1.2 equiv) and TMEDA (103 μL, 0.689 mmol, 2.4 equiv) in THF (2.5 mL) at −78° C. The resulting reaction solution was stirred at −78° C. for 30 min. A solution of ester S24-5 (124.2 mg, 0.287 mmol, 1.0 equiv) in THF (1.5 mL) was added via cannula. The resulting dark red solution was then stirred at −78° C. for 30 min, and cooled to −100° C. using a EtOH/liquid N₂ bath. A solution of enone (125 mg, 0.258 mmol, 0.9 equiv) in THF (1.5 mL) was added to the reaction mixture via cannula. The resulting reaction mixture was allowed to warm up to −78° C. over 30 min. LHMDS solution (1.0 M, 287 μL, 0.287 mmol, 1.0 equiv) was added. Then the reaction mixture was warmed up to −10° C. naturally over 40 min, quenched with saturated NH₄Cl and pH=7 buffer (1:1, 30 mL), and extracted with EtOAc (40 mL). The organic phase was separated, dried (Na₂SO₄), filtered and concentrated. The residue was purified by flash-column chromatography (5-30% ethyl acetate-hexanes) to afford the desired product S24-6 as a pale yellow solid (201.6 mg, 95%). ¹H NMR (400 MHz, CDCl₃) δ 15.67 (s, 1H), 7.49-7.46 (m, 2H), 7.38-7.29 (m, 3H), 5.59 (s, 1H), 5.35, 5.32 (ABq, J=12.2 Hz, 2H), 3.97 (d, J=10.4 Hz, 1H), 3.73 (s, 3H), 3.37 (s, 3H), 3.31 (s, 3H), 3.28 (dd, J=4.9, 15.9 Hz, 1H), 3.02-2.95 (m, 1H), 2.55-2.42 (m, 9H), 2.13 (d, J=12.0 Hz, 1H), 1.51 (s, 9H), 0.82 (s, 9H), 0.25 (s, 3H), 0.11 (s, 3H); MS (ESI) m/z 821.51 (M+H).

Compound S24-6 (201.6 mg, 0.246 mmol, 1.0 equiv) was dissolved in a premixed solution of 6 N HCl (0.34 mL) and THF (3.66 mL). The resulting reaction solution was stirred at rt for 40 min, and diluted with EtOAc (40 mL). The resulting mixture was washed with sat. NaHCO₃ (10 mL), and then brine (10 mL). The organic phase was then dried over Na₂SO₄. The dried solution was filtered and concentrated to give the desired product S24-7 as a yellow foamy solid (204.2 mg). ¹H NMR (400 MHz, CDCl₃) δ 15.55 (s, 1H), 10.33 (s, 1H), 7.50-7.47 (m, 3H), 7.38-7.31 (m, 3H), 5.36, 5.32 (ABq, J=12.2 Hz, 2H), 3.94 (d, J=11.0 Hz, 1H), 3.86 (s, 3H), 3.33 (dd, J=4.9, 15.9 Hz, 1H), 3.07-2.99 (m, 1H), 2.59-2.44 (m, 9H), 2.16 (d, J=14.6 Hz, 1H), 1.51 (s, 9H), 0.82 (s, 9H), 0.25 (s, 3H), 0.11 (s, 3H); MS (ESI) m/z 775.44 (M+H).

Hexamethyleneimine (8.5 μL, 0.072 mmol, 3.0 equiv), acetic acid (4 μL, 0.072 mmol, 3.0 equiv) and sodium triacetoxyborohydride (10 mg, 0.048 mmol, 2.0 equiv) were added sequentially to a solution of compound S24-7 (one tenth of the above crude product, 0.024 mmol, 1.0 equiv) in 1,2-dichloroethane (1 mL) at 23° C. After stirring for 2 h, the reaction mixture was quenched by the addition of saturated aqueous sodium bicarbonate and brine (1:1, 20 mL) and extracted with dichloromethane (2×20 mL). The combined organic extracts were dried over anhydrous sodium sulfate, filtered, and concentrated. The crude reductive amination product was used directly for the deprotection steps.

Aqueous HF (48-50%, 0.3 mL) was added to a solution of the compound in THF (0.6 mL) in a polypropylene reaction vessel at 23° C. The mixture was stirred vigorously at 23° C. overnight and poured into aqueous K₂HPO₄ (3.6 g dissolved in 30 mL water). The mixture was extracted with EtOAc (50 mL). The organic phase was dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was used directly in the next step without further purification.

Pd—C (10 wt %, 6.7 mg) was added in one portion into the yellow solution of the above crude product in a mixture of HCl/MeOH (0.5 N, 96 μL, 2.0 equiv) and MeOH (1 mL) at 23° C. The reaction vessel was sealed and purged with hydrogen by briefly evacuating the flask followed by flushing with hydrogen gas (1 atm). The resulting mixture was stirred at 23° C. for 30 min. The reaction mixture was then filtered through a small Celite pad. The filtrate was concentrated. The residue was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 5→60% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 7.1-8.4 min, were collected and freeze-dried to yield compound S24-9-1 (10.89 mg, 72% for 3 steps). ¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 7.07 (s, 1H), 4.43 (d, J=12.8 Hz, 1H), 4.29 (d, J=13.3 Hz, 1H), 4.12 (s, 1H), 3.73 (s, 3H), 3.47-3.41 (m, 2H), 3.26-3.20 (m, 3H), 3.05-2.97 (m, 8H), 2.38 (t, J=14.6 Hz, 1H), 2.28-2.25 (m, 1H), 2.00-1.82 (m, 4H), 1.73 (s, 4H), 1.70-1.60 (m, 1H); MS (ESI) m/z 556.33 (M+H).

Sodium triacetoxyborohydride (60 mg, 0.284 mmol, 2.0 equiv) was added to a solution of compound S24-7 (110 mg, 0.142 mmol, 1.0 equiv) in 1,2-dichloroethane (1 mL) at 23° C. After stirring overnight, more sodium triacetoxyborohydride (60 mg, 0.284 mmol, 2.0 equiv) was added. The resulting reaction mixture was stirred at rt overnight, quenched by the addition of saturated aqueous sodium bicarbonate and brine (1:1, 40 mL) and extracted with dichloromethane (3×20 mL). The combined organic extracts were dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was purified by flash-column chromatography (8-66% ethyl acetate-hexanes) to afford the desired product S24-8 (70.2 mg, 64%). ¹H NMR (400 MHz, CDCl₃) δ 15.69 (s, 1H), 7.48-7.47 (m, 2H), 7.38-7.29 (m, 3H), 7.16 (s, 1H), 5.34 (s, 2H), 4.79, 4.69 (dABq, J=4.9, 14.0 Hz, 2H), 3.97 (d, J=10.4 Hz, 1H), 3.71 (s, 3H), 3.27 (dd, J=4.9, 15.3 Hz, 1H), 3.01-2.94 (m, 1H), 2.55-2.42 (m, 9H), 2.15-2.11 (m, 2H), 1.53 (s, 9H), 0.82 (s, 9H), 0.25 (s, 3H), 0.11 (s, 3H); MS (ESI) m/z 777.50 (M+H).

Compound S24-10 was prepared from compound S24-8 by following the general HF deprotection procedure and Pd/C hydrogenation procedure: ¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 6.92 (s, 1H), 4.66 (s, 2H), 4.08 (s, 1H), 3.64 (s, 3H), 3.33 (s, 1H), 3.18 (dd, J=4.6, 15.6 Hz, 1H), 3.03-2.92 (m, 8H), 2.28 (t, J=14.6 Hz, 1H), 2.24-2.19 (m, 1H), 1.67-1.57 (m, 1H); MS (ESI) m/z 475.24 (M+H).

Ms₂O (13 mg, 0.075 mmol, 2.0 equiv) was added to a solution of alcohol S24-8 (29 mg, 0.037 mmol, 1.0 equiv) in THF (0.5 mL). Then TEA (10.4 μL, 0.075 mmol, 2.0 equiv) was added. To another reaction flask charged with a suspension of 7-azabicyclo[2,2,1]heptane-HCl (15 mg, 0.112 mmol, 3.0 equiv) in THF (0.5 mL) was added TEA (16 μL, 0.112 mmol, 3.0 equiv) and aqueous NaOH solution (8 N, 18 μL). The reaction mixtures were stirred for 30 min and mixed. The resulting reaction mixture was stirred at rt for 3 overnights, and diluted with EtOAc (50 mL), washed with pH=7 phosphate buffer solution. The organic phase was separated, dried over anhydrous sodium sulfate. The dried solution was filtered and concentrated. The crude product was used directly for the deprotection reactions. MS (ESI) m/z 856.69 (M+H).

Aqueous HF (48-50%, 0.3 mL) was added to a solution of the above compound in THF (0.6 mL) in a polypropylene reaction vessel at 23° C. The mixture was stirred vigorously at 23° C. overnight and poured into aqueous K₂HPO₄ (3.6 g dissolved in 30 mL water). The mixture was extracted with EtOAc (50 mL). The organic phase was dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was used directly in the next step without further purification.

Pd—C (10 wt %, 15 mg) was added in one portion into the yellow solution of the above crude product in a mixture of HCl/MeOH (0.5 N, 148 μL, 2.0 equiv) and MeOH (1 mL) at 23° C. The reaction vessel was sealed and purged with hydrogen by briefly evacuating the flask followed by flushing with hydrogen gas (1 atm). The resulting mixture was stirred at 23° C. for 40 min. The reaction mixture was then filtered through a small Celite pad. The filtrate was concentrated. The residue was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 20→100% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 7.05-7.88 min, were collected and concentrated to yield compound S24-9-2 (2.66 mg, 11% for 3 steps). ¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 7.05 (s, 1H), 4.31, 4.19 (ABq, J=12.8 Hz, 2H), 4.09-4.07 (m, 3H), 3.72 (s, 3H), 3.23-3.20 (m, 1H), 3.04-2.96 (m, 8H), 2.39-2.23 (m, 4H), 2.03-2.01 (m, 2H), 1.89-1.78 (m, 4H), 1.70-1.60 (m, 1H); MS (ESI) m/z 554.47 (M+H).

Neopentylamine (5.6 μL, 0.048 mmol, 2.0 equiv), acetic acid (2.7 μL, 0.048 mmol, 2.0 equiv) and sodium triacetoxyborohydride (7.6 mg, 0.036 mmol, 1.5 equiv) were added sequentially to a solution of compound S24-7 (one tenth of the above crude product, 0.024 mmol, 1.0 equiv) in 1,2-dichloroethane (1 mL) at 23° C. After stirring for 3 h, formaldehyde (11 μL, 0.14 mmol, 6.0 equiv), acetic acid (4.1 μL, 0.072 mmol, 3.0 equiv), and sodium triacetoxyborohydride (20 mg, 0.096 mmol, 4.0 equiv) were added. The resulting reaction mixture was stirred at rt for 1 h, and quenched by the addition of saturated aqueous sodium bicarbonate and brine (1:1, 20 mL) and extracted with dichloromethane (2×20 mL). The combined organic extracts were dried over anhydrous sodium sulfate, filtered, and concentrated. The crude product was used directly for the deprotection steps (see procedure for S24-9-1) to give the desired product S24-9-3 (10.97 mg, 72% for four steps). ¹H NMR (400 MHz, CD₃OD, hydrochloride, a mixture of isomers) δ 7.08 (s, 0.6H), 7.06 (s, 0.4H), 4.59 (d, J=12.8 Hz, 0.6H), 4.43, 4.39 (ABq, J=12.8 Hz, 0.8H), 4.22 (d, J=12.8 Hz, 0.6H), 4.13 (s, 1H), 3.78 (s, 1.2H), 3.73 (s, 1.8H), 3.26-2.95 (m, 14H), 2.46-2.35 (m, 1H), 2.30-2.26 (m, 1H), 1.71-1.61 (m, 1H), 1.04 (s, 5.4H), 1.02 (s, 3.6H); MS (ESI) m/z 558.36 (M+H).

The following compounds were prepared similarly to S24-9-1, S24-9-2 or S24-9-3 by using the corresponding amines and aldehydes.

¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 7.01 (s, 1H), 4.48 (d, J=12.8 Hz, 1H), 4.31 (d, J=12.8 Hz, 1H), 4.12 (s, 1H), 3.76-3.66 (m, 5H), 3.22 (dd, J=4.1, 15.6 Hz, 1H), 3.12-2.98 (m, 9H), 2.86-2.75 (m, 3H), 2.39 (t, J=14.2 Hz, 1H), 2.29-2.25 (m, 1H), 1.87-1.52 (m, 7H); MS (ESI) m/z 568.34 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 7.01 (s, 1H), 4.38 (d, J=13.7 Hz, 1H), 4.23 (d, J=13.7 Hz, 1H), 4.13 (s, 1H), 3.76 (s, 3H), 3.23 (dd, J=4.1, 15.6 Hz, 1H), 3.08-2.98 (m, 8H), 2.86 (s, 2H), 2.39 (t, J=14.2 Hz, 1H), 2.30-2.25 (m, 1H), 1.71-1.61 (m, 1H), 1.04 (s, 9H); MS (ESI) m/z 544.34 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 7.05 (s, 1H), 4.37 (d, J=13.3 Hz, 1H), 4.24 (d, J=12.8 Hz, 1H), 4.12 (s, 1H), 3.72 (s, 3H), 3.51-3.43 (m, 2H), 3.22 (dd, J=4.6, 15.6 Hz, 1H), 3.10-2.98 (m, 10H), 2.40 (t, J=14.2 Hz, 1H), 2.28-2.25 (m, 1H), 1.90-1.87 (m, 2H), 1.71-1.61 (m, 2H), 1.52-1.42 (m, 2H), 0.99 (d, J=6.4 Hz, 3H); MS (ESI) m/z 556.34 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 7.00 (s, 1H), 4.23 (d, J=12.8 Hz, 1H), 4.12 (s, 1H), 4.09 (d, J=12.8 Hz, 1H), 3.75 (s, 3H), 3.24 (dd, J=4.6, 15.6 Hz, 1H), 3.06-2.98 (m, 8H), 2.39 (t, J=14.2 Hz, 1H), 2.28-2.24 (m, 1H), 1.71-1.61 (m, 1H), 1.47 (s, 9H); MS (ESI) m/z 530.35 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride, a mixture of isomers) δ 7.01, 6.99 (s, 1H), 4.92 (d, J=12.8 Hz, 0.6H), 4.60 (d, J=13.3 Hz, 0.4H), 4.13, 4.12 (s, 1H), 3.96 (d, J=12.8 Hz, 0.4H), 3.80 (d, J=12.8 Hz, 0.6H), 3.77, 3.73 (s, 3H), 3.24 (dd, J=4.6, 15.6 Hz, 1H), 3.27-3.20 (m, 1H), 3.06-2.98 (m, 8H), 2.69 (s, 3H), 2.47-2.40 (m, 1H), 2.28-2.26 (m, 1H), 1.71-1.64 (m, 1H), 1.55 (s, 9H); MS (ESI) m/z 544.32 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 7.02 (s, 1H), 4.36, 4.30 (ABq, J=13.3 Hz, 1H), 4.10 (s, 1H), 3.77 (s, 3H), 3.24 (dd, J=3.7, 15.6 Hz, 1H), 3.05-2.94 (m, 9H), 2.40 (t, J=14.6 Hz, 1H), 2.27-2.24 (m, 1H), 1.71-1.61 (m, 1H), 1.34 (d, J=6.9 Hz, 3H), 1.00 (s, 9H); MS (ESI) m/z 558.34 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride, a mixture of isomers) δ 7.02 (s, 1H), 4.54 (d, J=12.4 Hz, 0.6H), 4.43 (d, J=12.8 Hz, 0.4H), 4.11, 4.10 (s, 1H), 4.02 (d, J=12.8 Hz, 0.4H), 3.88 (d, J=12.4 Hz, 0.6H), 3.78 (s, 1.2H), 3.74 (s, 1.8H), 3.44-3.39 (m, 2H), 3.26-3.18 (m, 1H), 3.05-2.97 (m, 8H), 2.46-2.35 (m, 1H), 2.29-2.24 (m, 1H), 2.12-2.07 (m, 2H), 2.02-1.97 (m, 2H), 1.71-1.58 (m, 4H), 1.44, 1.43 (s, 3H); MS (ESI) m/z 556.36 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 7.02 (s, 1H), 4.50 (d, J=12.8 Hz, 1H), 4.32 (d, J=13.3 Hz, 1H), 4.12 (s, 1H), 3.74 (s, 3H), 3.55-3.50 (m, 2H), 3.25-3.20 (m, 3H), 3.05-2.97 (m, 8H), 2.39 (t, J=14.6 Hz, 1H), 2.28-2.24 (m, 1H), 2.17-2.03 (m, 4H), 1.70-1.60 (m, 1H); MS (ESI) m/z 528.30 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 7.03 (s, 1H), 4.64 (d, J=13.3 Hz, 1H), 4.13 (s, 1H), 4.08 (d, J=12.8 Hz, 1H), 3.75 (s, 3H), 3.66-3.60 (m, 1H), 3.43-3.37 (m, 1H), 3.27-3.21 (m, 2H), 3.06-2.98 (m, 8H), 2.44-2.34 (m, 2H), 2.28-2.25 (m, 1H), 2.13-2.07 (m, 1H), 2.03-1.97 (m, 1H), 1.81-1.61 (m, 2H), 1.49 (d, J=6.4 Hz, 3H); MS (ESI) m/z 542.36 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 7.00 (s, 1H), 4.32 (d, J=13.7 Hz, 1H), 4.18 (d, J=13.7 Hz, 1H), 4.12 (s, 1H), 3.74 (s, 3H), 3.23 (dd, J=4.6, 15.6 Hz, 1H), 3.08-2.97 (m, 8H), 2.91 (d, J=7.3 Hz, 2H), 2.38 (t, J=14.6 Hz, 1H), 2.29-2.24 (m, 1H), 2.11-2.04 (m, 1H), 1.71-1.61 (m, 1H), 1.04 (dd, J=0.9, 6.9 Hz, 6H); MS (ESI) m/z 530.39 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 6.98 (s, 1H), 4.78 (dt, J=47.2, 4.6 Hz, 2H), 4.38 (d, J=13.7 Hz, 1H), 4.25 (d, J=13.7 Hz, 1H), 4.12 (s, 1H), 3.74 (s, 3H), 3.45 (dt, J=26.6, 4.6 Hz, 2H), 3.22 (dd, J=4.6, 15.6 Hz, 1H), 3.07-2.97 (m, 8H), 2.38 (t, J=14.2 Hz, 1H), 2.28-2.24 (m, 1H), 1.70-1.60 (m, 1H); MS (ESI) m/z 520.31 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride, a mixture of isomers) δ 7.01 (s, 1H), 4.62 (d, J=12.8 Hz, 0.6H), 4.43 (d, J=13.3 Hz, 0.4H), 4.29 (d, J=12.8 Hz, 0.4H), 4.12 (s, 1H), 4.04 (d, J=12.8 Hz, 0.6H), 3.76, 3.74 (s, 3H), 3.26-3.18 (m, 1H), 3.15-2.98 (m, 10H), 2.83 (s, 1.2H), 2.78 (s, 1.8H), 2.46-2.35 (m, 1H), 2.29-2.19 (m, 2H), 1.71-1.60 (m, 1H), 1.11-1.01 (m, 6H); MS (ESI) m/z 544.36 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride, a mixture of isomers) δ 7.03 (s, 1H), 4.96-4.83 (m, 2H), 4.73 (d, J=13.3 Hz, 0.5H), 4.60 (d, J=13.3 Hz, 0.5H), 4.50 (d, J=13.3 Hz, 0.5H), 4.38 (d, J=13.3 Hz, 0.5H), 4.12 (s, 1H), 3.76 (s, 3H), 3.72-3.57 (m, 2H), 3.25-3.16 (m, 3H), 3.05-2.97 (m, 8H), 2.40 (t, J=14.6 Hz, 1H), 2.28-2.25 (m, 1H), 1.70-1.60 (m, 1H), 1.22-1.20 (m, 1H), 0.82-0.78 (m, 2H), 0.48-0.46 (m, 2H); MS (ESI) m/z 574.36 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride, a mixture of isomers) δ 7.04, 7.00 (s, 1H), 4.76 (d, J=13.3 Hz, 0.8H), 4.30 (s, 0.4H), 4.11 (s, 1H), 3.98 (d, J=12.8 Hz, 0.8H), 3.76, 3.74 (s, 3H), 3.37-3.30 (m, 1H), 3.22 (dd, J=4.1, 15.6 Hz, 1H), 3.05-2.91 (m, 10H), 2.41 (t, J=13.7 Hz, 1H), 2.27-2.24 (m, 1H), 2.02-1.98 (m, 2H), 1.86-1.82 (m, 2H), 1.72-1.61 (m, 6.4H), 1.46 (d, J=6.9 Hz, 0.6H); MS (ESI) m/z 556.41 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 7.00 (s, 1H), 4.21 (d, J=12.8 Hz, 1H), 4.11 (s, 1H), 4.07 (d, J=12.8 Hz, 1H), 3.74 (s, 3H), 3.24 (dd, J=4.6, 15.6 Hz, 1H), 3.07-2.97 (m, 8H), 2.38 (t, J=14.6 Hz, 1H), 2.28-2.24 (m, 1H), 1.81 (q, J=7.8 Hz, 2H), 1.70-1.61 (m, 1H), 1.41 (s, 6H), 1.04 (t, J=7.3 Hz, 3H); MS (ESI) m/z 544.36 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride, a mixture of isomers) δ 7.01 (s, 1H), 4.72 (d, J=12.8 Hz, 0.7H), 4.60 (d, J=12.8 Hz, 0.3H), 4.12, 4.11 (s, 1H), 3.96 (d, J=12.8 Hz, 0.3H), 3.81 (d, J=12.8 Hz, 0.7H), 3.77, 3.73 (s, 3H), 3.26-3.19 (m, 1 H), 3.05-2.98 (m, 8H), 2.68 (s, 3H), 2.48-2.32 (m, 1H), 2.28-2.24 (m, 1H), 1.94 (q, J=7.3 Hz, 2H), 1.72-1.62 (m, 1H), 1.50, 1.48 (s, 6H), 1.11-1.07 (m, 3H); MS (ESI) m/z 558.39 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 7.02 (s, 1H), 4.34 (d, J=12.8 Hz, 1H), 4.20 (d, J=12.8 Hz, 1H), 4.12 (s, 1H), 3.75 (s, 3H), 3.24 (dd, J=4.1, 15.6 Hz, 1H), 3.05-2.97 (m, 8H), 2.38 (t, J=14.6 Hz, 1H), 2.29-2.24 (m, 1H), 1.70-1.61 (m, 1H), 1.32 (s, 3H), 1.31 (s, 3H), 1.24-1.18 (m, 1H), 0.73-0.68 (m, 2H), 0.62-0.58 (m, 2H); MS (ESI) m/z 556.39 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride, a mixture of isomers) δ 7.03 (s, 1H), 4.92-4.85 (m, 0.6H), 4.76 (d, J=12.8 Hz, 0.4H), 4.13, 4.12 (s, 1H), 4.04 (d, J=12.8 Hz, 0.4H), 3.88 (d, J=12.8 Hz, 0.6H), 3.77 (s, 1.2H), 3.74 (s, 1.8H), 3.28-3.20 (m, 1H), 3.06-2.98 (m, 8H), 2.76 (s, 3H), 2.48-2.33 (m, 1H), 2.29-2.26 (m, 1H), 1.72-1.62 (m, 1H), 1.42 (s, 3H), 1.37 (s, 3H), 1.32-1.29 (m, 1H), 0.83-0.74 (m, 2H), 0.69-0.66 (m, 2H); MS (ESI) m/z 570.39 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 7.04 (s, 1H), 4.53 (d, J=13.7 Hz, 1H), 4.32 (d, J=13.7 Hz, 1H), 4.13 (s, 1H), 3.75 (s, 3H), 3.73-3.69 (m, 2H), 3.23 (dd, J=4.6, 16.0 Hz, 1H), 3.08-2.97 (m, 8H), 2.40 (t, J=14.6 Hz, 1H), 2.31-2.23 (m, 3H), 1.79-1.73 (m, 2H), 1.71-1.61 (m, 1H), 1.38 (d, J=6.9 Hz, 3H), 1.32 (d, J=6.4 Hz, 3H); MS (ESI) m/z 556.43 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 7.05 (s, 1H), 4.37, 4.24 (ABq, J=13.3 Hz, 2H), 4.12 (s, 1H), 3.72 (s, 3H), 3.45 (br t, J=13.7 Hz, 2H), 3.22 (dd, J=4.1, 15.1 Hz, 1H), 3.07-2.97 (m, 10H), 2.39 (t, J=14.6 Hz, 1H), 2.27-2.24 (m, 1H), 1.93-1.90 (m, 2H), 1.82-1.75 (m, 3H), 1.70-1.61 (m, 1H), 1.56-1.50 (m, 1H); MS (ESI) m/z 542.41 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride) δ 7.06 (s, 1H), 4.42 (d, J=12.8 Hz, 1H), 4.28 (d, J=12.8 Hz, 1H), 4.12 (s, 1H), 3.73 (s, 3H), 3.38-3.30 (m, 2H), 3.25-3.17 (m, 3H), 3.05-2.98 (m, 8H), 2.39 (t, J=14.6 Hz, 1H), 2.27-2.25 (m, 1H), 1.76-1.60 (m, 5H), 1.09 (s, 3H), 1.03 (s, 3H); MS (ESI) m/z 570.39 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride, a mixture of isomers) δ 7.01, 6.99 (s, 1H), 4.66, 4.58 (ABq, J=15.1 Hz, 0.67H), 4.43, 4.31 (ABq, J=15.1 Hz, 1.33H), 4.12 (s, 1H), 3.74, 3.70 (s, 3H), 3.59-3.55 (m, 1.33H), 3.46-3.40 (m, 0.67H), 3.24-3.20 (m, 1H), 3.04-2.97 (m, 8H), 2.39 (t, J=14.2 Hz, 1H), 2.27-2.24 (m, 1H), 1.98-1.76 (m, 4H), 1.70-1.58 (m, 3H), 1.50 (d, J=6.0 Hz, 1H), 1.46 (d, J=6.0 Hz, 1H), 1.32 (d, J=6.4 Hz, 2H), 1.24 (d, J=6.4 Hz, 2H); MS (ESI) m/z 570.52 (M+H).

¹H NMR (400 MHz, CD₃OD, hydrochloride, a mixture of isomers) δ 7.07-7.01 (m, 1H), 4.54-4.24 (m, 2H), 4.11 (s, 1H), 3.92-3.89 (m, 1H), 3.75 (s, 3H), 3.77-3.67 (m, 1H), 3.26-3.21 (m, 1H), 3.07-2.97 (m, 8H), 2.44-2.37 (m, 2H), 2.26-2.22 (m, 2H), 1.92-1.65 (m, 3H), 1.43-1.30 (m, 6H); MS (ESI) m/z 556.49 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.10 (s, 1H), 4.46 (d, J=13.2 Hz, 1H), 4.33 (d, J=12.8 Hz, 1H), 4.13 (s, 1H), 4.04-4.01 (m, 2H), 3.84-3.74 (m, 2H), 3.72 (s, 3H), 3.41 (t, J=13.2 Hz, 2H), 3.27-2.97 (m, 11H), 2.40 (dd, J=14.8, 14.4 Hz, 1H), 2.28 (ddd, J=13.6, 5.6, 2.8 Hz, 1H), 1.65 (ddd, J=13.2, 13.2, 13.2 Hz, 1H); MS (ESI) m/z 544.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 1H), 4.31 (d, J=13.6 Hz, 1H), 4.17 (d, J=13.6 Hz, 1H), 4.14 (s, 1H), 3.74 (s, 3H), 3.26-2.98 (m, 11H), 2.37 (dd, J=14.8, 14.4 Hz, 1H), 2.28 (ddd, J=13.2, 5.6, 2.8 Hz, 1H), 1.76-1.63 (m, 3H), 1.47-1.38 (m, 2H), 0.98 (t, J=7.2 Hz, 3H); MS (ESI) m/z 530.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 1H), 4.55, 4.08 (d, J=12.8 Hz, 1H total), 4.31 (dd, J=12.8, 12.8 Hz, 1H), 4.11 (s, 1H), 3.72 (s, 3H), 3.28-2.96 (m, 11H), 2.79, 2.76 (s, 3H total), 2.39-2.23 (m, 2H), 1.80-1.60 (m, 3H), 1.44-1.30 (m, 2H), 0.99-0.91 (m, 3H); MS (ESI) m/z 544.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.94 (s, 1H), 4.52 (d, J=13.2 Hz, 1H), 4.34 (d, J=13.2 Hz, 1H), 4.25-4.13 (m, 5H), 3.74 (s, 3H), 3.24-2.98 (m, 9H), 2.61-2.58 (m, 1H), 2.44-2.25 (m, 3H), 1.65 (ddd, J=13.6, 13.6, 13.6 Hz, 1H), MS (ESI) m/z 514.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.06 (s, 1H), 4.72, 4.60 (d, J=12.8 Hz, 1H total), 3.98, 3.82 (d, J=12.8 Hz, 1H total), 4.14-4.13 (m, 1H), 3.74, 3.73 (s, 3H total), 3.28-2.99 (m, 9H), 2.69 (s, 3H), 2.41 (dd, J=14.8, 14.8 Hz, 1H), 2.29 (ddd, J=11.2, 4.8, 2.4 Hz, 1H), 1.97-1.92 (m, 2H), 1.72-1.60 (m, 1H), 1.48 (d, J=7.2 Hz, 6H), 1.09 (t, J=7.2 Hz, 3H); MS (ESI) m/z 558.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.06 (s, 1H), 4.38 (d, J=12.8 Hz, 1H), 4.25 (d, J=13.2 Hz, 1H), 4.13 (s, 1H), 3.72 (s, 3H), 3.55-5.43 (m, 2H), 3.31 (s, 3H), 3.27-2.98 (m, 13H), 2.39 (dd, J=14.4, 14.4 Hz, 1H), 2.27 (ddd, J=13.6, 5.6, 2.8 Hz, 1H), 1.97-1.90 (m, 3H), 1.71-1.50 (m, 3H); MS (ESI) m/z 586.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.07, 6.99 (s, 1H total), 4.34-4.31 (m, 1H), 4.21-4.17 (m, 1H), 4.12 (s, 1H), 3.77, 3.73 (s, 3H total), 3.26-2.98 (m, 11H), 2.38 (dd, J=15.2, 14.0 Hz, 1H), 2.29-2.10 (m, 2H), 1.96-1.90 (m, 2H), 1.73-1.66 (m, 5H), 1.32-1.22 (m, 2H); MS (ESI) m/z 556.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.09, 7.01 (s, 1H total), 4.66-4.63 (m, 1H), 4.43-4.33 (m, 1H), 4.13-4.06 (m, 1H), 3.79-3.73 (m, 3H), 3.27-2.98 (m, 14H), 2.84-2.14 (m, 3H), 1.98-1.94 (m, 2H), 1.74-1.65 (m, 5H), 1.33-1.12 (m, 2H); MS (ESI) m/z 570.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.02 (s, 1H), 4.52, 4.32 (d, J=13.2 Hz, 1H total), 4.45, 4.22 (d, J=13.2 Hz, 1H total), 4.12 (s, 1H), 3.76, 3.75 (s, 3H total), 3.25-2.98 (m, 13H), 2.42-2.25 (m, 3H), 1.95-1.91 (m, 2H), 1.72-1.62 (m, 5H), 1.41-1.35 (m, 3H), 1.29-1.19 (m, 2H); MS (ESI) m/z 584.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.06, 7.04 (s, 1H total), 4.39 (dd, J=13.2, 5.2 Hz, 1H), 4.27 (d, J=13.2 Hz, 1H), 4.12 (s, 1H), 4.05 (s, 1H), 3.72 (s, 3H), 3.54-3.47 (m, 1H), 3.34-3.31 (m, 2H), 3.24-2.98 (m, 10H), 2.40 (dd, J=14.4, 14.4 Hz, 1H), 2.25 (ddd, J=14.4, 5.2, 2.8 Hz, 1H), 2.14-2.10 (m, 1H), 1.98-1.88 (m, 2H), 1.71-1.61 (m, 2H); MS (ESI) m/z 558.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.98 (s, 1H), 4.41 (d, J=13.6 Hz, 1H), 4.27 (d, J=13.2 Hz, 1H), 4.13 (s, 1H), 3.75 (s, 3H), 3.24-2.97 (m, 9H), 2.81-2.79 (m, 1H), 2.37 (dd, J=14.4, 14.4 Hz, 1H), 2.27 (ddd, J=12.8, 5.2, 2.8 Hz, 1H), 1.65 (ddd, J=12.8, 12.8, 12.8 Hz, 1H), 0.91 (d, J=5.2 Hz, 4H); MS (ESI) m/z 514.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.04 (s, 1H), 4.64-4.53 (m, 1H), 4.72-4.34 (m, 1H), 4.13 (s, 1H), 3.76 (s, 3H), 3.25-2.94 (m, 13H), 2.40 (dd, J=14.0, 14.0 Hz, 1H), 2.27 (ddd, J=13.2, 5.2, 2.8 Hz, 1H), 1.66 (ddd, J=11.2, 11.2, 11.2 Hz, 1H), 1.02-0.80 (m, 4H); MS (ESI) m/z 528.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.05, 7.03 (s, 1H total), 4.61, 4.42 (d, J=12.8 Hz, 1H total), 4.51, 4.35 (d, J=13.2 Hz, 1H total), 4.12 (s, 1H), 3.75 (s, 3H), 3.39-3.33 (m, 2H), 3.25-2.90 (m, 9H), 2.89-2.85 (m, 1H), 2.40 (dd, J=14.8, 14.4 Hz, 1H), 2.26 (ddd, J=13.6, 4.8, 2.8 Hz, 1H), 1.67 (ddd, J=11.2, 11.2, 11.2 Hz, 1H), 1.46 (t, J=7.2 Hz, 3H), 1.04-0.67 (m, 4H); MS (ESI) m/z 542.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.06, 7.05 (s, 1H total), 4.59-4.52 (m, 1H), 4.50-4.40 (m, 2H), 4.15 (s, 1H), 3.81-3.74 (m, 4H), 3.59-3.48 (m, 2H), 3.24-2.98 (m, 10H), 2.60-2.50 (m, 1H), 2.38 (dd, J=13.2, 12.8 Hz, 1H), 2.28 (ddd, J=12.4, 5.2, 2.8 Hz, 1H), 2.14-2.07 (m, 1H), 1.98, 1.95 (s, 3H total), 1.70-1.61 (m, 1H); MS (ESI) m/z 585.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 1H), 4.31 (d, J=13.6 Hz, 1H), 4.18 (d, J=13.6 Hz, 1H), 4.13 (s, 1H), 3.74 (s, 3H), 3.26-2.98 (m, 11H), 2.38 (dd, J=14.8, 14.4 Hz, 1H), 2.27 (ddd, J=13.2, 5.2, 2.8 Hz, 1H), 1.72-1.60 (m, 4H), 0.97 (d, J=6.4 Hz, 6H); MS (ESI) m/z 544.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.02, 7.01 (s, 1H total), 4.58, 4.09 (d, J=12.8 Hz, 1H total), 4.36 (dd, J=12.8 Hz, 1H), 4.13 (s, 1H), 3.75 (s, 3H), 3.25-2.98 (m, 11H), 2.81, 2.79 (s, 3H total), 2.40 (dd, J=14.0, 13.6 Hz, 1H), 2.28 (ddd, J=12.4, 5.2, 2.8 Hz, 1H), 1.71-1.61 (m, 4H), 0.96 (d, J=6.0 Hz, 6H); MS (ESI) m/z 558.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.03 (s, 1H), 4.46 (d, J=13.2 Hz, 1H), 4.26 (d, J=12.4 Hz, 1H), 4.13 (s, 1H), 3.75 (s, 3H), 3.26-2.98 (m, 13H), 2.44-2.36 (m, 1H), 2.30-2.26 (m, 1H), 1.71-1.60 (m, 4H), 1.37 (dd, J=7.2, 7.2 Hz, 3H), 0.97-0.95 (m, 6H); MS (ESI) m/z 572.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.96 (s, 1H), 4.35 (d, J=13.6 Hz, 1H), 4.21 (d, J=14.0 Hz, 1H), 4.12 (s, 1H), 3.74 (s, 3H), 3.72-3.65 (m, 3H), 3.25-2.97 (m, 11H), 2.37 (dd, J=14.8, 14.4 Hz, 1H), 2.26 (ddd, J=13.6, 5.2, 2.8 Hz, 1H), 1.64 (ddd, J=13.2, 13.2, 13.2 Hz, 1H), 1.19 (d, J=6.0 Hz, 6H); MS (ESI) m/z 560.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.65, 4.37 (d, J=13.2, Hz, 1H total), 4.47, 4.19 (d, J=13.2 Hz, 1H total), 4.12 (s, 1H), 3.82-3.79 (m, 2H), 3.75, 3.74 (s, 3H total), 3.71-3.67 (m, 1H), 3.42-3.33 (m, 2H), 3.24-2.97 (m, 9H), 2.87, 2.83 (s, 3H total), 2.39 (dd, J=14.8, 14.4 Hz, 1H), 2.27 (ddd, J=12.0, 5.2, 2.8 Hz, 1H), 1.65 (ddd, J=13.2, 13.2, 13.2 Hz, 1H), 1.12 (d, J=6.0 Hz, 6H); MS (ESI) m/z 574.1

¹H NMR (400 MHz, CD₃OD) δ 7.03 (s, 1H), 4.59, 4.40 (d, J=13.6 Hz, 1H total), 4.52, 4.33 (d, J=13.6 Hz, 1H total), 4.13 (s, 1H), 3.78-3.76 (m, 5H), 3.71-3.66 (m, 1H), 3.45-3.34 (m, 2H), 3.25-2.98 (m, 11H), 2.40 (dd, J=13.6, 13.6 Hz, 1H), 2.27 (ddd, J=12.0, 5.2, 2.8 Hz, 1H), 1.66 (ddd, J=13.2, 13.2, 13.2 Hz, 1H), 1.42-1.34 (m, 3H), 1.21-1.19 (m, 6H); MS (ESI) m/z 588.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 1H), 4.31 (d, J=13.6 Hz, 1H), 4.17 (d, J=13.6, Hz, 1H), 4.13 (s, 1H), 3.74 (s, 3H), 3.25-2.90 (m, 11H), 2.38 (dd, J=14.4, 14.4 Hz, 1H), 2.29-2.26 (m, 1H), 1.83-1.64 (m, 7H), 1.37-1.20 (m, 3H), 1.06-0.95 (m, 2H); MS (ESI) m/z 570.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.09-7.00 (m, 1H), 4.63, 4.30 (d, J=12.8 Hz, 1H total), 4.43, 4.04 (d, J=13.2 Hz, 1H total), 4.14 (s, 1H), 3.81-3.74 (m, 3H), 3.25-2.79 (m, 14H), 2.40 (dd, J=15.6, 14.8 Hz, 1H), 2.30-2.27 (m, 1H), 1.95-1.64 (m, 7H), 1.36-1.28 (m, 3H), 1.06-0.95 (m, 2H); MS (ESI) m/z 584.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.50, 4.30 (d, J=13.2 Hz, 1H total), 4.43, 4.19 (d, J=13.6 Hz, 1H total), 4.11 (s, 1H), 3.76, 3.75 (s, 3H total), 3.25-2.98 (m, 13H), 2.45-2.36 (m, 1H), 2.28-2.25 (m, 1H), 1.92-1.62 (m, 7H), 1.41-1.14 (m, 6H), 1.06-0.95 (m, 2H); MS (ESI) m/z 598.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.50, 4.08 (d, J=12.8 Hz, 1H total), 4.34, 4.30 (d, J=13.6 Hz, 1H total), 4.14 (s, 1H), 3.80, 3.75 (s, 3H total), 3.27-2.74 (m, 15H), 2.44-2.12 (m, 4H), 2.05-1.80 (m, 4H), 1.65 (ddd, J=13.2, 13.2, 13.2 Hz, 1H); MS (ESI) m/z 556.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.40 (t, J=13.2 Hz, 1H), 4.20 (d, J=12.8 Hz, 1H), 4.13 (s, 1H), 3.75 (s, 3H), 3.23-2.83 (m, 14H), 2.40 (dd, J=14.8, 13.6 Hz, 1H), 2.28 (ddd, J=12.4, 5.2, 2.8 Hz, 1H), 2.20-2.14 (m, 2H), 2.04-1.97 (m, 1H), 1.92-1.84 (m, 3H), 1.66 (ddd, J=13.2, 13.2, 13.2 Hz, 1H), 1.39-1.30 (m, 3H); MS (ESI) m/z 570.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 6.34 (t, J=53.6 Hz, 1H), 4.43 (d, J=13.6 Hz, 1H), 4.30 (d, J=13.2 Hz, 1H), 4.12 (s, 1H), 3.75 (s, 3H), 3.63 (ddd, J=13.6, 13.6, 13.6, 2H), 3.25-2.98 (m, 9H), 2.39 (dd, J=14.4, 14.8 Hz, 1H), 2.27 (ddd, J=12.4, 5.2, 2.8 Hz, 1H), 1.65 (ddd, J=13.2, 13.2, 13.2 Hz, 1H); MS (ESI) m/z 538.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.04 (s, 1H), 6.46 (t, J=53.6 Hz, 1H), 4.60 (d, J=13.2 Hz, 1H), 4.41 (d, J=13.2 Hz, 1H), 4.12 (s, 1H), 3.78-3.72 (m, 5H), 3.36-3.34 (m, 2H), 3.25-2.98 (m, 9H), 2.41 (dd, J=14.4, 14.4 Hz, 1H), 2.27 (ddd, J=12.0, 5.2, 2.8 Hz, 1H), 1.66 (ddd, J=12.4, 12.4, 12.4 Hz, 1H), 1.41 (t, J=7.2 Hz, 3H); MS (ESI) m/z 565.9 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.45 (d, J=13.2 Hz, 1H), 4.33 (d, J=13.2 Hz, 1H), 4.11 (s, 1H), 4.06 (t, J=8.8 Hz, 2H), 3.75 (s, 3H), 3.26-2.97 (m, 9H), 2.39 (dd, J=14.4, 14.4 Hz, 1H), 2.26 (ddd, J=13.2, 5.2, 2.8 Hz, 1H), 1.66 (ddd, J=14.0, 14.0, 14.0 Hz, 1H); MS (ESI) m/z 556.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.03 (s, 1H), 4.52 (d, J=13.2 Hz, 1H), 4.35 (d, J=12.8 Hz, 1H), 4.20-4.12 (m, 3H), 3.74 (s, 3H), 3.26-2.92 (m, 12H), 2.40 (dd, J=14.8, 14.8 Hz, 1H), 2.27 (ddd, J=15.2, 5.2, 2.8 Hz, 1H), 1.66 (ddd, J=13.2, 13.2, 13.2 Hz, 1H); MS (ESI) m/z 570.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.04 (s, 1H), 4.59 (d, J=13.6 Hz, 1H), 4.43 (d, J=13.6 Hz, 1H), 4.21-4.12 (m, 3H), 3.74 (s, 3H), 3.26-2.98 (m, 11H), 2.40 (dd, J=14.8, 14.4 Hz, 1H), 2.27 (ddd, J=13.2, 5.2, 2.8 Hz, 1H), 1.66 (ddd, J=13.2, 13.2, 13.2 Hz, 1H); 1.18-1.15 (m, 1H), 0.78 (d, J=7.6 Hz, 2H), 0.42 (d, J=4.4 Hz, 2H); MS (ESI) m/z 610.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.43 (dd, J=13.2, 13.2 Hz, 1H), 4.23 (dd, J=12.8, 12.8 Hz, 1H), 4.11 (s, 1H), 3.73 (s, 3H), 3.28-2.96 (m, 13H), 2.38-2.24 (m, 2H), 1.76-1.60 (m, 3H), 1.41-1.31 (m, 5H), 0.99-0.91 (m, 3H); MS (ESI) m/z 558.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.05 (s, 1H), 6.49 (t, J=53.2 Hz, 1H), 4.70 (d, J=13.2 Hz, 1H), 4.51 (d, J=13.6 Hz, 1H), 4.13 (s, 1H), 3.85-3.76 (m, 5H), 3.25-2.98 (m, 11H), 2.41 (dd, J=14.8, 14.4 Hz, 1H), 2.27 (ddd, J=13.2, 5.2, 2.8 Hz, 1H), 1.66 (ddd, J=13.6, 13.6, 13.6 Hz, 1H), 1.29-1.24 (m, 1H), 0.82 (d, J=7.6 Hz, 2H), 0.48 (d, J=4.8 Hz, 2H); MS (ESI) m/z 592.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.97 (s, 1H), 4.28 (d, J=13.6 Hz, 1H), 4.16-4.12 (m, 2H), 3.74 (s, 3H), 3.25-2.98 (m, 11H), 2.77-2.69 (m, 1H), 2.37 (dd, J=14.8, 14.4 Hz, 1H), 2.27 (ddd, J=13.6, 5.2, 2.8 Hz, 1H), 2.20-2.16 (m, 2H), 2.03-1.80 (m, 4H), 1.65 (ddd, J=14.0, 14.0, 14.0 Hz, 1H); MS (ESI) m/z 542.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.63, 4.22 (d, J=13.2 Hz, 1H total), 4.40 (dd, J=13.6 Hz, 1H), 4.13 (s, 1H), 3.76 (s, 3H), 3.25-2.98 (m, 13H), 2.88-2.82 (m, 1H), 2.40 (dd, J=14.4, 14.4 Hz, 1H), 2.29-2.14 (m, 3H), 2.03-1.80 (m, 4H), 1.65 (ddd, J=14.0, 14.0, 14.0 Hz, 1H), 1.22-1.12 (m, 1H), 0.83-0.76 (m, 2H), 0.48-0.40 (m, 2H); MS (ESI) m/z 596.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.62, 4.28 (d, J=13.2 Hz, 1H total), 4.43, 4.03 (d, J=13.2 Hz, 1H total), 4.12 (s, 1H), 3.77, 3.74 (s, 3H total), 3.26-2.98 (m, 11H), 2.83, 2.79 (s, 3H total), 2.40 (dd, J=14.8, 14.8 Hz, 1H), 2.27 (ddd, J=13.6, 5.2, 2.8 Hz, 1H), 1.99-1.92 (m, 1H), 1.80-1.62 (m, 5H), 1.42-1.20 (m, 3H), 1.10-0.90 (m, 2H); MS (ESI) m/z 584.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.94 (s, 1H), 4.31 (d, J=13.6 Hz, 1H), 4.15 (d, J=14.4 Hz, 1H), 4.11 (s, 1H), 3.74 (s, 3H), 3.25-2.97 (m, 9H), 2.74 (s, 3H), 2.38 (dd, J=14.4, 14.8 Hz, 1H), 2.26 (ddd, J=13.6, 5.2, 2.8 Hz, 1H), 1.66 (ddd, J=13.6, 13.6, 13.6 Hz, 1H), MS (ESI) m/z 488.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.53-7.47 (m, 5H), 6.93 (s, 1H), 4.32-4.25 (m, 3H), 4.14 (d, J=13.2 Hz, 1H), 4.11 (s, 1H), 3.59 (s, 3H), 3.22-2.97 (m, 9H), 2.36 (dd, J=14.8, 14.4 Hz, 1H), 2.25 (ddd, J=14.0, 5.2, 2.8 Hz, 1H), 1.64 (ddd, J=13.2, 13.2, 13.2 Hz, 1H); MS (ESI) m/z 564.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.53-7.49 (m, 5H), 6.88 (s, 1H), 4.51-4.48 (m, 1H), 4.15-4.11 (m, 2H), 3.91 (d, J=13.2 Hz, 1H), 3.47 (s, 3H), 3.17-2.97 (m, 9H), 2.36-2.23 (m, 2H), 1.73 (d, J=6.8 Hz, 3H), 1.68-1.58 (m, 1H); MS (ESI) m/z 578.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.54-7.48 (m, 5H), 6.90 (s, 1H), 4.49-4.46 (m, 1H), 4.11 (s, 1H), 3.98 (dd, J=13.2, 12.8 Hz, 2H), 3.39 (s, 3H), 3.21-2.97 (m, 9H), 2.36-2.23 (m, 2H), 1.71 (d, J=6.4 Hz, 3H), 1.68-1.59 (m, 1H); MS (ESI) m/z 578.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.55, 4.10 (d, J=13.2 Hz, 1H total), 4.35 (dd, J=13.2, 13.2 Hz, 1H), 4.12 (s, 1H), 3.75 (s, 3H), 3.34-2.98 (m, 11H), 2.81, 2.78 (s, 3H total), 2.45-2.35 (m, 1H), 2.29-2.25 (m, 1H), 1.66 (ddd, J=12.8, 12.8, 12.8 Hz, 1H), 1.43-1.35 (m, 3H); MS (ESI) m/z 516.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.60-7.53 (m, 5H), 6.98, 6.95 (s, 1H total), 4.53, 4.03 (d, J=12.8 Hz, 1H), 4.42-4.25 (m, 3H), 4.13 (s, 1H), 3.52, 3.32 (s, 3H total), 3.18-2.97 (m, 9H), 2.83, 2.81 (s, 3H total), 2.37-2.24 (m, 2H), 1.63 (ddd, J=12.4, 12.4, 12.4 Hz, 1H); MS (ESI) m/z 578.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.57-7.49 (m, 5H), 6.95, 6.85 (s, 1H total), 4.54-4.10 (m, 5H), 3.55, 3.34 (s, 3H total), 3.26-2.97 (m, 11H), 2.38-2.22 (m, 2H), 1.64 (ddd, J=13.2, 13.2, 13.2 Hz, 1H), 1.46-1.41 (m, 3H); MS (ESI) m/z 592.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.59-7.48 (m, 5H), 6.97, 6.86 (s, 1H total), 4.68, 4.28 (d, J=12.8 Hz, 1H total), 4.59 (dd, J=12.8, 12.8 Hz, 1H), 4.42-4.33 (m, 2H), 4.13, 4.12 (s, 1H total), 3.57, 3.35 (s, 3H total), 3.17-2.98 (m, 11H), 2.37-2.25 (m, 2H), 1.64 (ddd, J=12.8, 12.8, 12.8 Hz, 1H), 1.29-1.26 (m, 1H), 0.82-0.80 (m, 2H), 0.44-0.42 (m, 2H); MS (ESI) m/z 618.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.54 (d, J=13.2 Hz, 1H), 4.36 (d, J=12.8 Hz, 1H), 4.12 (s, 1H), 3.78 (s, 3H), 3.26-2.98 (m, 9H), 2.39 (dd, J=14.4, 14.4 Hz, 1H), 2.28-2.25 (m, 1H), 1.71-1.57 (m, 2H), 1.03-1.01 (m, 2H), 0.81-0.79 (m, 2H), 0.75-0.71 (m, 2H), 0.43-0.40 (m, 2H); MS (ESI) m/z 554.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.06 (s, 1H), 4.70, 4.46 (d, J=12.8 Hz, 1H total), 4.61 (s, 1H), 4.13, 4.12 (s, 1H total), 3.80, 3.76 (s, 3H total), 3.25-2.94 (m, 12H), 2.46-2.25 (m, 2H), 1.70-1.61 (m, 2H), 1.14-1.10 (m, 2H), 0.96-0.70 (m, 4H), 0.40-0.31 (m, 2H); MS (ESI) m/z 568.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.07 (s, 1H), 4.67 (dd, J=14.4, 14.0 Hz, 1H), 4.52 (dd, J=12.4, 12.4 Hz, 1H), 4.13 (s, 1H), 3.77, 3.75 (s, 3H total), 3.69-3.61 (m, 1H), 3.48-3.43 (m, 1H), 3.26-2.98 (m, 9H), 2.40 (dd, J=13.6, 13.6 Hz, 1H), 2.29-2.25 (m, 1H), 1.71-1.61 (m, 2H), 1.39-1.36 (m, 3H), 1.29-1.22 (m, 1H), 0.96-0.70 (m, 5H), 0.42-0.31 (m, 2H); MS (ESI) m/z 582.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.24 (d, J=12.8 Hz, 1H), 4.14-4.10 (m, 2H), 3.76 (s, 3H), 3.27-2.98 (m, 9H), 2.39 (dd, J=14.8, 14.4 Hz, 1H), 2.27 (ddd, J=13.6, 4.8, 2.8 Hz, 1H), 1.98-1.80 (m, 8H), 1.66 (ddd, J=13.6, 13.6, 13.6 Hz, 1H), 1.50 (s, 3H); MS (ESI) m/z 556.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.24 (d, J=12.8 Hz, 1H), 4.13-4.18 (m, 2H), 3.75 (s, 3H), 3.27-2.97 (m, 9H), 2.39 (dd, J=14.8, 14.8 Hz, 1H), 2.27 (ddd, J=13.6, 4.8, 2.4 Hz, 1H), 1.97-1.91 (m, 2H), 1.80-1.53 (m, 8H), 1.50 (s, 3H), 1.32-1.25 (m, 1H); MS (ESI) m/z 570.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.70-7.67 (m, 2H), 7.57-7.47 (m, 3H), 6.87 (s, 1H), 4.13 (s, 1H), 3.81 (dd, J=12.8 Hz, 2H), 3.34 (s, 3H), 3.19-2.98 (m, 9H), 2.35-2.23 (m, 2H), 1.88 (d, J=6.8 Hz, 6H), 1.63 (ddd, J=13.6, 13.6, 13.6 Hz, 1H); MS (ESI) m/z 592.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 1H), 4.45 (d, J=12.8 Hz, 1H), 4.25 (d, J=13.2 Hz, 1H), 4.11 (s, 1H), 3.75 (s, 3H), 3.25-2.98 (m, 9H), 2.90 (s, 3H), 2.85 (s, 3H), 2.40 (dd, J=14.8, 14.4 Hz, 1H), 2.27-2.24 (m, 1H), 1.66 (ddd, J=13.2, 13.2, 13.2 Hz, 1H); MS (ESI) m/z 502.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.02 (s, 1H), 4.43 (d, J=13.6 Hz, 1H), 4.29 (d, J=13.6 Hz, 1H), 4.11 (s, 1H), 4.06-3.99 (m, 2H), 3.72 (s, 3H), 3.26-2.97 (m, 11H), 2.39 (dd, J=14.8, 14.4 Hz, 1H), 2.26 (ddd, J=13.6, 4.8, 2.8 Hz, 1H), 1.66 (ddd, J=13.6, 13.6, 13.6 Hz, 1H), 1.36 (t, J=7.2 Hz, 3H); MS (ESI) m/z 584.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.29 (d, J=13.2 Hz, 1H), 4.15 (d, J=13.6 Hz, 1H), 4.13 (s, 1H), 3.75 (s, 3H), 3.50-3.44 (m, 1H), 3.26-2.98 (m, 9H), 2.38 (dd, J=14.8, 14.4 Hz, 1H), 2.29-2.25 (m, 1H), 1.66 (ddd, J=13.6, 13.6, 13.6 Hz, 1H), 1.40 (d, J=6.4 Hz, 6H); MS (ESI) m/z 516.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.03 (s, 1H), 6.46 (t, J=53.2 Hz, 1H), 4.60 (d, J=13.2 Hz, 1H), 4.39 (d, J=13.6 Hz, 1H), 4.11 (s, 1H), 3.82-3.74 (m, 5H), 3.26-2.95 (m, 12H), 2.42 (dd, J=14.8, 14.8 Hz, 1H), 2.28-2.24 (m, 1H), 1.67 (ddd, J=13.2, 13.2, 13.2 Hz, 1H); MS (ESI) m/z 552.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.00, 6.99 (s, 1H total), 4.41 (d, J=12.8 Hz, 1H), 4.27 (d, J=13.2 Hz, 1H), 4.06 (s, 1H), 3.65 (s, 3H), 3.51-3.47 (m, 1H), 3.28-3.25 (m, 2H), 3.17-3.12 (m, 1H), 2.98-2.90 (m, 9H), 2.30 (dd, J=14.4, 14.4 Hz, 1H), 2.21-2.17 (m, 1H), 1.93-1.88 (m, 1H), 1.80-1.73 (m, 1H), 1.62-1.40 (m, 5H), 0.82-0.73 (m, 6H); MS (ESI) m/z 584.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.99 (s, 1H), 4.34-4.29 (m, 1H), 4.22-4.15 (m, 1H), 4.03 (s, 1H), 3.64 (s, 3H), 3.51-3.41 (m, 2H), 3.27 (s, 3H), 3.17-2.90 (m, 12H), 2.30 (dd, J=14.4, 14.4 Hz, 1H), 2.19-2.14 (m, 2H), 2.03-1.99 (m, 1H), 1.92-1.80 (m, 1H), 1.61-1.52 (m, 2H); MS (ESI) m/z 572.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.90 (s, 1H), 4.20 (dd, J=13.6, 5.2 Hz, 1H), 4.09-4.02 (m, 2H), 3.66 (s, 3H), 3.16-2.88 (m, 10H), 2.29 (dd, J=14.4, 14.4 Hz, 1H), 2.19-2.16 (m, 1H), 1.85-1.80 (m, 1H), 1.58-1.51 (m, 2H), 1.29 (d, J=6.4 Hz, 3H), 0.94 (t, J=7.6 Hz, 3H); MS (ESI) m/z 530.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.33 (d, J=13.2 Hz, 1H), 4.19-4.14 (m, 2H), 3.74 (s, 3H), 3.24-2.98 (m, 11H), 2.45-2.38 (m, 3H), 2.29-2.26 (m, 1H), 2.03-1.93 (m, 5H), 1.67-1.54 (m, 2H), 1.34-1.32 (m, 1H), 1.24-1.20 (m, 3H), 1.02-0.97 (m, 3H); MS (ESI) m/z 610.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.30 (s, 4H), 6.98 (s, 1H), 4.67-4.62 (m, 5H), 4.48 (d, J=13.2 Hz, 1H), 4.03 (s, 1H), 3.66 (s, 3H), 3.19-3.15 (m, 1H), 2.97-2.80 (m, 8H), 2.32 (dd, J=14.4, 14.4 Hz, 1H), 2.17 (ddd, J=14.4, 5.2, 2.8 Hz, 1H), 1.58 (ddd, J=13.2, 13.2, 13.2 Hz, 1H); MS (ESI) m/z 576.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.94 (s, 1H), 4.53-4.47 (m, 1H), 4.40-4.32 (m, 1H), 4.28-4.19 (m, 1H), 4.12 (s, 1H), 3.89-3.85 (m, 2H), 3.73 (s, 3H), 3.24-2.98 (m, 11H), 2.38 (dd, J=14.8, 14.8 Hz, 1H), 2.27-2.24 (m, 1H), 1.70-1.61 (m, 1H), 1.32, 1.25 (d, J=7.2, 7.2 Hz, 3H total); MS (ESI) m/z 528.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.07 (s, 1H), 4.63 (d, J=13.2 Hz, 1H), 4.47 (d, J=13.2 Hz, 1H), 4.14 (s, 1H), 3.96-3.91 (m, 2H), 3.80-3.72 (m, 5H), 3.26-2.98 (m, 9H), 2.70-2.66 (m, 2H), 2.39 (dd, J=14.4, 14.4 Hz, 1H), 2.30-2.26 (m, 1H), 1.66 (ddd, J=13.2, 13.2, 13.2 Hz, 1H); MS (ESI) m/z 563.9 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.92 (s, 1H), 4.36 (d, J=13.2 Hz, 1H), 4.11 (d, J=13.2 Hz, 1H), 4.03 (s, 1H), 3.68 (s, 3H), 3.18-2.90 (m, 9H), 2.63 (dd, J=6.4, 6.4 Hz, 1H), 2.31 (dd, J=14.4, 14.4 Hz, 1H), 2.20-2.16 (m, 1H), 1.58 (ddd, J=13.2, 13.2, 13.2 Hz, 1H), 1.39 (d, J=6.8 Hz, 3H), 1.01-0.99 (m, 1H), 0.72-0.62 (m, 2H), 0.51-0.47 (m, 1H), 0.32-0.27 (m, 1H); MS (ESI) m/z 542.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.95 (s, 1H), 4.59-4.50 (m, 1H), 4.04-3.98 (m, 2H), 3.71, 3.67 (s, 3H total), 3.14-2.60 (m, 13H), 2.34-2.29 (m, 1H), 2.19-2.16 (m, 1H), 1.60-1.52 (m, 1H), 1.48-1.42 (m, 3H), 1.14-1.08 (m, 1H), 0.78-0.60 (m, 2H), 0.42-0.31 (m, 2H); MS (ESI) m/z 556.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.96, 6.95 (s, 1H total), 4.62-4.49 (m, 1H), 4.24-4.14 (m, 1H), 4.02 (s, 1H), 3.73-3.67 (m, 3H), 3.18-2.81 (m, 12H), 2.34-2.31 (m, 1H), 2.21-2.18 (m, 1H), 1.63-1.54 (m, 1H), 1.44-1.39 (m, 3H), 1.26-1.12 (m, 4H), 0.80-0.60 (m, 2H), 0.48-0.30 (m, 2H); MS (ESI) m/z 570.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.30-7.22 (m, 4H), 7.07, 7.05 (s, 1H total), 4.57-4.47 (m, 1H), 4.33-4.30 (m, 2H), 4.13 (s, 1H), 3.78-3.75 (m, 3H), 3.59-3.52 (m, 1H), 3.50-3.40 (m, 3H), 3.26-2.98 (m, 9H), 2.79, 2.78 (s, 3H total), 2.45-2.38 (m, 1H), 2.30-2.26 (m, 1H), 1.70-1.61 (m, 1H); MS (ESI) m/z 604.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.30-7.22 (m, 4H), 7.06, 7.04 (s, 1H total), 4.47-4.24 (m, 3H), 4.13 (s, 1H), 3.75 (s, 3H), 3.57-3.41 (m, 4H), 3.26-2.98 (m, 11H), 2.41-2.36 (m, 1H), 2.30-2.26 (m, 1H), 1.70-1.61 (m, 1H), 1.40-1.36 (m, 3H); MS (ESI) m/z 618.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.09, 7.06 (s, 1H total), 4.56-4.49 (m, 1H), 4.40-4.35 (m, 1H), 4.12 (s, 1H), 3.78, 3.75 (s, 3H total), 3.56-3.55 (m, 1H), 3.43-3.34 (m, 2H), 3.24-2.89 (m, 10H), 2.56-2.54 (m, 1H), 2.42-2.36 (m, 2H), 2.39-2.25 (m, 1H), 1.74-1.38 (m, 9H); MS (ESI) m/z 582.3 (M+H)

¹H NMR (400 MHz, CD₃OD) δ 7.38-7.20 (m, 5H), 7.10 (s, 1H), 4.45 (d, J=13.2 Hz, 1H), 4.32 (d, J=12.8 Hz, 1H), 4.12 (s, 1H), 3.75, 3.74 (s, 3H total), 3.65-3.58 (m, 2H), 3.29-3.22 (m, 3H), 3.05-2.89 (m, 9H), 2.45-2.42 (m, 1H), 2.28-2.23 (m, 1H), 2.08-1.95 (m, 4H), 1.72-1.66 (m, 1H); MS (ESI) m/z 618.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.64, 4.20 (d, J=12.4 Hz, 1H total), 4.45-4.43 (m, 1H), 4.11 (s, 1H), 3.74 (s, 3H), 3.23-2.88 (m, 16H), 2.44-2.40 (m, 1H), 2.27-2.23 (m, 1H), 1.69-1.60 (m, 1H); MS (ESI) m/z 534.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.02 (s, 1H), 4.54 (d, J=12.8 Hz, 1H), 4.34 (d, J=13.6 Hz, 1H), 4.10 (s, 1H), 3.74 (s, 3H), 3.61-3.54 (m, 2H), 3.04-2.96 (m, 13H), 2.43-2.36 (m, 1H), 2.26-2.23 (m, 1H), 1.70-1.60 (m, 1H), 1.41-1.35 (m, 3H); MS (ESI) m/z 548.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.02 (s, 1H), 4.32 (d, J=−13.2 Hz, 1H), 4.19 (d, J=13.6 Hz, 1H), 4.13 (s, 1H), 3.76 (s, 3H), 3.25-2.98 (m, 10H), 2.42-2.35 (m, 1H), 2.29-2.26 (m, 1H), 2.19-2.13 (m, 1H), 1.70-1.61 (m, 1H), 1.31 (d, J=6.8 Hz, 3H), 1.04 (d, J=7.2 Hz, 3H), 0.94 (d, J=7.2 Hz, 3H); MS (ESI) m/z 544.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.02 (s, 1H), 4.35 (d, J=13.2 Hz, 1H), 4.22 (d, J=13.6 Hz, 1H), 4.15 (s, 1H), 3.77 (s, 3H), 3.28-3.01 (m, 11H), 2.41 (dd, J=14.4, 14.8 Hz, 1H), 2.32-2.28 (m, 1H), 1.70-1.66 (m, 3H), 1.00 (s, 9H); MS (ESI) m/z 558.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.03-6.69 (m, 1H), 4.58, 4.19 (d, J=13.6 Hz, 1H total), 4.43-4.31 (m, 1H), 4.13 (s, 1H), 3.75, 3.74 (s, 3H total), 3.26-2.98 (m, 11H), 2.81, 2.80 (s, 3H total), 2.45-2.39 (m, 1H), 2.35-2.26 (m, 1H), 1.77-1.65 (m, 3H), 1.02-0.96 (m, 9H); MS (ESI) m/z 572.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.94 (s, 1H), 4.37 (dd, J=13.2, 7.6 Hz, 1H), 4.18 (dd, J=13.6, 4.0 Hz, 1H), 4.02 (s, 1H), 3.66 (s, 3H), 3.17-2.88 (m, 13H), 2.36-2.27 (m, 1H), 2.18-2.15 (m, 1H), 1.62-1.53 (m, 3H), 1.28 (q, J=7.6 Hz, 3H), 0.86 (d, J=5.2 Hz, 9H); MS (ESI) m/z 586.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.89 (s, 1H), 4.21 (d, J=13.6 Hz, 1H), 4.07 (d, J=13.6 Hz, 1H), 4.02 (s, 1H), 3.64 (s, 3H), 3.21-2.82 (m, 11H), 2.29 (dd, J=14.4, 14.8 Hz, 1H), 2.20-2.15 (m, 1H), 1.90-1.80 (m, 1H), 1.70-1.43 (m, 11H), 1.23-1.18 (m, 2H); MS (ESI) m/z 584.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.02 (d, J=6.4 Hz, 1H), 4.44, 4.33 (d, J=13.2 Hz, 1H total), 4.21-4.06 (m, 2H), 4.08 (d, J=12.0 Hz, 3H), 3.32-2.82 (m, 14H), 2.42 (dd, J=14.8, 14.8 Hz, 1H), 2.31-2.28 (m, 1H), 2.14-1.55 (m, 12H), 1.32-1.20 (m, 2H); MS (ESI) m/z 598.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.93 (s, 1H), 4.39 (dd, J=13.6, 13.6 Hz, 1H), 4.20, 4.12 (d, J=13.2 Hz, 1H total), 4.02 (s, 1H), 3.69, 3.67 (s, 3H total), 3.18-2.89 (m, 13H), 2.38-2.28 (m, 1H), 2.19-2.16 (m, 1H), 1.90-1.50 (m, 12H), 1.46-1.38 (m, 3H), 1.34-1.20 (m, 2H); MS (ESI) m/z 612.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.39-4.28 (m, 1H), 4.25-4.22 (m, 1H), 4.13 (s, 1H), 3.78 (s, 3H), 3.29-3.00 (m, 11H), 2.46-2.39 (m, 1H), 2.30-2.27 (m, 1H), 1.74-1.65 (m, 1H), 1.20-1.15 (m, 1H), 0.77 (d, J=6.8 Hz, 2H), 0.46 (d, J=5.6 Hz, 2H); MS (ESI) m/z 528.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.05, 7.04 (s, 1H total), 4.53 (d, J=12.8 Hz, 1H), 4.34 (d, J=12.8 Hz, 1H), 4.16 (s, 1H), 3.78, 3.77 (s, 3H total), 3.28-3.01 (m, 11H), 2.88, 2.83 (s, 3H total), 2.42 (dd, J=13.2, 13.2 Hz, 1H), 2.32-2.29 (m, 1H), 1.73-1.64 (m, 1H), 1.30-1.25 (m, 1H), 0.83 (t, J=8.8 Hz, 2H), 0.52-0.45 (m, 2H); MS (ESI) m/z 542.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.06 (m, 1H), 4.67, 4.29 (d, J=13.6 Hz, 1H total), 4.47 (dd, J=13.2, 13.2 Hz, 1H), 4.17 (s, 1H), 3.80 (s, 3H), 3.29-3.02 (m, 13H), 2.48-2.33 (m, 2H), 1.75-1.65 (m, 1H), 1.44-1.36 (m, 3H), 1.28-1.21 (m, 1H), 0.88-0.81 (m, 2H), 1.50-0.47 (m, 2H); MS (ESI) m/z 556.1 (M+H)

¹H NMR (400 MHz, CD₃OD) δ 7.43-7.38 (m, 5H), 6.88 (s, 1H), 4.28 (d, J=13.6 Hz, 1H), 4.16-4.11 (m, 2H), 3.62 (s, 3H), 3.30-3.03 (m, 11H), 2.41-2.28 (m, 2H), 1.74-1.64 (m, 1H), 1.48 (s, 6H); MS (ESI) m/z 606.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.51 (m, 5H), 6.88-6.85 (m, 1H), 4.30 (d, J=12.8 Hz, 1H), 4.20-4.15 (m, 1H), 3.93-3.89 (m, 1H), 3.81-3.60 (m, 6H), 3.25-3.01 (m, 11H), 2.45-2.18 (m, 2H), 1.73-1.64 (m, 1H), 1.57-1.46 (m, 6H); MS (ESI) m/z 620.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.53 (t, J=8.0 Hz, 2H), 7.44 (d, J=6.4 Hz, 2H), 7.38-7.32 (m, 1H), 6.90, 6.80 (s, 1H total), 4.35-4.01 (m, 3H), 3.64 (s, 3H), 3.54-3.50 (m, 1H), 3.24-3.01 (m, 12H), 2.43-2.30 (m, 2H), 1.72-1.64 (m, 1H), 1.54 (d, J=12.4 Hz, 3H), 1.45 (d, J=13.2 Hz, 3H), 1.30 (d, J=6.4 Hz, 3H); MS (ESI) m/z 634.0 (M+H)

¹H NMR (400 MHz, CD₃OD) δ 7.06 (s, 1H), 4.36 (d, J=13.2 Hz, 1H), 4.22 (d, J=13.2 Hz, 1H), 4.16 (s, 1H), 3.70 (s, 3H), 3.31-3.02 (m, 10H), 2.43 (dd, J=14.8, 14.4 Hz, 1H), 2.33-2.29 (m, 1H), 2.24-2.19 (m, 1H), 1.70 (dd, J=13.6, 13.6 Hz, 1H), 1.35 (d, J=6.8 Hz, 3H), 1.06 (dd, J=6.8, 6.8 Hz, 6H); MS (ESI) m/z 544.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.07, 7.05 (s, 1H total), 4.67 (d, J=12.8 Hz, 1H), 4.23-4.11 (m, 2H), 3.84-3.80 (m, 3H), 3.31-3.03 (m, 13H), 2.49-2.20 (m, 3H), 1.75-1.69 (m, 1H), 1.48-1.40 (m, 3H), 1.20-1.13 (m, 6H); MS (ESI) m/z 558.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.09, 7.06 (s, 1H total), 4.62, 4.22 (d, J=13.6 Hz, 1H total), 4.44 (dd, J=13.6, 13.6 Hz, 1H), 4.18 (s, 1H), 3.83, 3.82 (s, 3H total), 3.30-3.03 (m, 12H), 2.48-2.25 (m, 3H), 1.75-1.66 (m, 1H), 1.45-1.31 (m, 6H), 1.17-1.02 (m, 6H); MS (ESI) m/z 572.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.09, 7.08 (s, 1H total), 4.58 (d, J=13.6 Hz, 1H), 4.39 (dd, J=13.6, 13.6 Hz, 1H), 4.18 (s, 1H), 3.84, 3.82 (s, 3H total), 3.33-3.06 (m, 12H), 2.53-2.43 (m, 1H), 2.34 (m, 1H), 1.78-1.69 (m, 1H), 1.56-1.46 (m, 6H), 1.40-1.35 (m, 3H); MS (ESI) m/z 544.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.15, 7.08 (s, 1H total), 4.52-4.51 (m, 2H), 4.06-4.03 (m, 1H), 3.94-4.82 (m, 4H), 3.28-3.05 (m, 10H), 2.45 (dd, J=14.4, 14.4 Hz, 1H), 2.35-2.32 (m, 1H), 2.25-2.19 (m, 1H), 1.77-1.68 (m, 1H), 1.12 (s, 9H); MS (ESI) m/z 574.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.09, 7.05 (s, 1H total), 4.80 (s, 2H), 4.20-4.15 (m, 3H), 3.93 (s, 3H), 3.84-3.55 (m, 2H), 3.28-3.05 (m, 10H), 2.46-2.39 (m, 2H), 1.76-1.67 (m, 1H), 1.57 (t, J=7.2 Hz, 2H), 1.46 (t, J=7.2 Hz, 1H), 1.28 (s, 3H), 1.03 (s, 6H); MS (ESI) m/z 602.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.05 (s, 1H), 4.57, 4.19 (d, J=13.2 Hz, 1H total), 4.44, 3.99 (d, J=13.2 Hz, 1H total), 4.14 (s, 1H), 3.80, 3.77 (s, 3H total), 3.70-3.62 (m, 1H), 3.28-3.00 (m, 9H), 2.73 (s, 3H), 2.49-2.27 (m, 2H), 1.74-1.63 (m, 1H), 1.52-1.40 (m, 6H); MS (ESI) m/z 530.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.05 (s, 1H), 4.36 (d, J=13.6 Hz, 1H), 4.26 (d, J=12.8 Hz, 1H), 4.17-4.04 (m, 5H), 3.92-3.82 (m, 5H), 3.28-3.04 (m, 10H), 2.50-2.40 (m, 2H), 1.78-1.68 (m, 1H); MS (ESI) m/z 544.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.10 (s, 1H), 4.53 (d, J=13.6 Hz, 1H), 4.32 (d, J=14.4 Hz, 1H), 4.22-4.17 (m, 4H), 3.92-3.88 (m, 1H), 3.82 (s, 3H), 3.27-3.05 (m, 10H), 2.85 (s, 3H), 2.53-2.31 (m, 4H), 1.78-1.68 (m, 1H); MS (ESI) m/z 557.9

¹H NMR (400 MHz, CD₃OD) δ 7.08 (s, 1H), 4.43-4.38 (m, 2H), 4.27-4.21 (m, 2H), 4.16-4.14 (m, 1H), 3.91-3.89 (m, 1H), 3.77-3.75 (m, 3H), 3.68-3.63 (m, 1H), 3.31-3.04 (m, 12H), 2.51-2.45 (m, 2H), 2.36-2.30 (m, 2H), 1.77-1.68 (m, 1H), 1.53-1.40 (m, 3H); MS (ESI) m/z 572.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.35 (d, J=14.0 Hz, 1H), 4.21 (d, J=13.6 Hz, 1H), 4.10 (s, 1H), 3.90-3.86 (m, 2H), 3.75 (s, 3H), 3.55-3.52 (m, 1H), 3.25-2.97 (m, 11H), 2.68-2.62 (m, 2H), 2.39 (dd, J=14.4, 14.4 Hz, 1H), 2.27-2.18 (m, 2H), 1.71-1.65 (m, 2H); MS (ESI) m/z 558.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.02 (s, 1H), 4.66-4.62 (m, 1H), 4.47-4.36 (m, 1H), 4.11 (s, 1H), 3.93-3.84 (m, 2H), 3.77 (s, 3H), 3.58-3.43 (m, 2H), 3.26-2.98 (m, 11H), 2.88-2.84 (m, 4H), 2.42 (dd, J=13.6, 13.6 Hz, 1H), 2.27-2.24 (m, 2H), 1.72-1.63 (m, 2H); MS (ESI) m/z 572.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.04, 7.03 (s, 1H total), 4.52-4.48 (m, 1H), 4.34-4.25 (m, 1H), 4.11 (s, 1H), 3.93-3.87 (m, 2H), 3.77 (m, 3H), 3.53-3.37 (m, 2H), 3.27-2.98 (m, 13H), 2.79-2.70 (m, 1H), 2.47-2.38 (m, 1H), 2.27-2.21 (m, 2H), 1.72-1.55 (m, 2H), 1.43-1.38 (m, 3H); MS (ESI) m/z 586.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.03, 6.98 (s, 1H total), 4.31-4.13 (m, 2H), 3.80-3.76 (m, 1H), 3.69 (s, 3H), 3.26-3.01 (m, 10H), 2.69 (s, 2H), 2.43-2.02 (m, 5H), 1.71-1.55 (m, 2H), 1.50-1.33 (m, 4H); MS (ESI) m/z 568.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.02, 6.95 (s, 1H total), 4.15 (d, J=13.2 Hz, 1H), 3.97-3.91 (m, 1H), 3.82-3.67 (m, 4H), 3.24-3.00 (m, 13H), 2.71, 2.68 (s, 3H total), 2.40-2.28 (m, 3H), 2.16-2.06 (m, 1H), 1.78-1.53 (m, 5H), 1.37-1.31 (m, 1H); MS (ESI) m/z 582.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.04-6.97 (m, 1H), 4.57-4.12 (m, 3H), 3.79-3.68 (m, 4H), 3.25-2.92 (m, 12H), 2.41-2.10 (m, 4H), 1.74-1.45 (m, 6H), 1.40-1.32 (m, 5H); MS (ESI) m/z 596.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.03, 7.02 (s, 1H total), 4.66, 4.47 (d, J=13.2 Hz, 1H total), 4.31, 4.10 (d, J=13.2 Hz, 1H total), 4.12 (s, 1H), 3.75, 3.74 (s, 3H total), 3.48-3.47 (m, 2H), 3.29-3.25 (m, 1H), 3.05-2.89 (m, 9H), 2.41-2.32 (m, 2H), 2.29-2.22 (m, 1H), 2.13-2.09 (m, 1H), 2.01-1.95 (m, 2H), 1.79-1.70 (m, 1H), 1.69-1.59 (m, 2H), 1.07-1.00 (m, 3H), MS (ESI) m/z 556.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.08 (s, 1H), 4.39 (d, J=12.0 Hz, 1H), 4.25 (d, J=14.4 Hz, 1H), 4.15 (s, 1H), 3.73 (s, 3H), 3.50 (t, J=14.4 Hz, 2H), 3.26-3.23 (m, 2H), 3.07-2.91 (m, 9H), 2.40 (dd, J=14.4, 14.4 Hz, 1H), 2.32-2.28 (m, 1H), 1.96-1.93 (m, 2H), 1.75-1.55 (m, 2H), 1.48-1.26 (m, 6H), 0.97-0.90 (m, 3H); MS (ESI) m/z 584.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.06 (s, 1H), 4.40-4.36 (m, 1H), 4.25 (d, J=12.8 Hz, 1H), 4.11 (s, 1H), 3.76-3.71 (m, 3H total), 3.38-3.33 (m, 2H), 3.28-3.21 (m, 2H), 3.07-2.89 (m, 9H), 2.63-2.58 (m, 1H), 2.39 (dd, J=14.4, 14.4 Hz, 1H), 2.28-2.25 (m, 1H), 1.97-1.94 (m, 1H), 1.87-1.81 (m, 1H), 1.67-1.61 (m, 1H), 1.17-1.13 (m, 1H), 0.99-0.92 (m, 6H); MS (ESI) m/z 570.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.07 (s, 1H), 4.37 (d, J=13.2 Hz, 1H), 4.24 (d, J=12.8 Hz, 1H), 4.14 (s, 1H), 3.76 (s, 3H), 3.55-3.48 (m, 2H), 3.28-2.99 (m, 11H), 2.39 (dd, J=14.8, 14.4 Hz, 1H), 2.30-2.26 (m, 1H), 1.96-1.92 (m, 2H), 1.87-1.32 (m, 5H), 0.92 (d, =7.2 Hz, 6H); MS (ESI) m/z 584.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.07 (s, 1H), 4.37 (d, J=12.8 Hz, 1H), 4.25 (d, J=12.8 Hz, 1H), 4.14 (s, 1H), 3.72 (s, 3H), 3.57-3.49 (m, 2H), 3.26-2.98 (m, 11H), 2.39 (dd, J=14.8, 14.4 Hz, 1H), 2.29-2.26 (m, 1H), 1.95-1.93 (m, 2H), 1.70-1.57 (m, 3H), 1.41-1.32 (m, 1H), 0.92 (s, 9H)); MS (ESI) m/z 598.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.29-7.21 (m, 4H), 7.02 (s, 1H), 4.37 (d, J=13.2 Hz, 1H), 4.24 (d, J=13.2, Hz, 1H), 4.17-4.12 (m, 2H), 3.77 (s, 3H), 3.48 (dd, J=7.6, 7.6 Hz, 2H), 3.26-2.97 (m, 11H), 2.38 (dd, J=14.8, 14.4 Hz, 1H), 2.28-2.25 (m, 1H), 1.67-1.61 (m, 1H); MS (ESI) m/z 590.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.11, 7.09 (s, 1H total), 4.41 (dd, J=13.2, 13.2 Hz, 1H), 4.28 (dd, J=13.2, 4.8 Hz, 1H), 4.14 (s, 1H), 3.79-3.72 (m, 3H), 3.56-3.48 (m, 2H), 3.28-2.80 (m, 11H), 2.40 (dd, J=14.4, 14.0 Hz, 1H), 2.30-2.27 (m, 1H), 2.06-1.93 (m, 1H), 1.88-1.80 (m, 1H), 1.68-1.52 (m, 2H), 1.48-1.40 (m, 1H), 1.10 (d, J=7.2 Hz, 3H), 0.98 (s, 3H); MS (ESI) m/z 570.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.09 (s, 1H), 4.44 (d, J=13.2 Hz, 1H), 4.32 (d, J=12.8 Hz, 1H), 4.14 (s, 1H), 3.76 (s, 3H), 3.67-3.60 (m, 2H), 3.28-3.00 (m, 11H), 2.68-2.60 (m, 1H), 2.42 (dd, J=14.8, 14.4 Hz, 1H), 2.30-2.27 (m, 1H), 2.20-2.10 (m, 2H), 1.97-1.91 (m, 2H), 1.73-1.64 (m, 1H); MS (ESI) m/z 610.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.04 (s, 1H), 4.57 (d, J=11.6 Hz, 1H), 4.39-4.30 (m, 2H), 4.13 (s, 1H), 3.75 (s, 3H), 3.52-3.45 (m, 1H), 3.25-2.98 (m, 11H), 2.67-2.60 (m, 1H), 2.42-2.25 (m, 2H), 2.17-2.02 (m, 2H), 1.97, 1.95 (s, 3H total), 1.67-1.60 (m, 1H); MS (ESI) m/z 585.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.96, 4.94 (s, 1H total), 4.56-4.50 (m, 1H), 4.45-4.35 (m, 3H), 4.12-4.00 (m, 4H), 3.75-3.73 (m, 3H), 3.49-3.46 (m, 3H), 3.21-2.98 (m, 9H), 2.42-2.36 (m, 1H), 1.68-1.60 (m, 1H); MS (ESI) m/z 544.2 (M+H).

S24-9-135:

MS (ESI) m/z 606.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.06 (s, 1H), 5.45 (d, J=52.8 Hz, 1H), 4.61-4.56 (m, 1H), 4.41-4.38 (m, 1H), 4.13 (s, 1H), 3.74 (s, 3H), 3.50-3.47 (m, 2H), 3.26-2.98 (m, 11H), 2.43-2.26 (m, 4H), 1.67-1.60 (m, 1H); MS (ESI) m/z 546.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.89 (s, 1H), 4.21 (d, J=13.2 Hz, 1H), 4.09-4.03 (m, 2H), 3.65 (s, 3H), 3.27-2.88 (m, 11H), 2.32-2.25 (m, 1H), 2.19-2.16 (m, 1H), 1.66-1.52 (m, 8H), 1.21-1.12 (m, 4H), 0.91-0.88 (m, 2H); MS (ESI) m/z 584.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.02, 7.01 (s, 1H total), 4.57 (d, J=12.8 Hz, 1H), 4.40-4.30 (m, 1H), 4.13 (s, 1H), 3.75, 3.74 (s, 3H total), 3.25-2.98 (m, 11H), 2.81, 2.79 (s, 3H total), 2.45-2.38 (m, 1H), 2.29-2.26 (m, 1H), 1.71-1.65 (m, 9H), 1.40-1.20 (m, 4H), 1.02-0.96 (m, 1H); MS (ESI) m/z 598.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.93 (s, 1H), 4.36 (d, J=13.2 Hz, 1H), 4.16 (d, J=13.2 Hz, 1H), 4.14 (s, 1H), 3.66 (s, 3H), 3.16-2.88 (m, 13H), 2.34-2.27 (m, 1H), 2.20-2.17 (m, 1H), 1.62-1.53 (m, 8H), 1.28-1.14 (m, 7H), 0.91-0.86 (m, 2H); MS (ESI) m/z 612.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.78 (d, J=12.8 Hz, 1H), 4.17 (d, J=13.2 Hz, 1H), 4.14 (s, 1H), 3.88-2.86 (m, 1H), 3.75 (s, 3H), 3.70-3.67 (m, 2H), 3.45 (s, 3H), 3.41-3.37 (m, 2H), 3.27-2.98 (m, 9H), 2.44-2.37 (m, 1H), 2.30-2.27 (m, 2H), 2.14-2.11 (m, 1H), 2.02-1.87 (m, 2H), 1.70-1.62 (m, 1H); MS (ESI) m/z 572.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.02, 7.01 (s, 1H total), 4.58 (d, J=13.2 Hz, 1H), 4.38-4.30 (m, 1H), 4.14 (s, 1H), 3.75, 3.74 (s, 3H total), 3.25-2.98 (m, 11H), 2.81, 2.79 (s, 3H total), 2.44-2.37 (m, 1H), 2.29-2.26 (m, 1H), 1.89-1.81 (m, 2H), 1.71-16 (m, 1H), 1.05-1.00 (m, 3H); MS (ESI) m/z 530.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.46 (dd, J=13.6, 70.2 Hz, 1H), 4.25 (dd, J=13.2, 10.8 Hz, 1H), 4.13 (s, 1H), 3.75 (s, 3H), 3.25-2.98 (m, 13H), 2.44-2.37 (m, 1H), 2.30-2.26 (m, 1H), 1.82-1.79 (m, 2H), 1.71-1.61 (m, 1H), 1.40-1.33 (m, 3H), 1.03-0.99 (m, 3H); MS (ESI) m/z 544.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.98 (s, 1H), 4.31 (d, J=13.6 Hz, 1H), 4.17 (d, J=13.6 Hz, 1H), 4.13 (s, 1H), 3.74 (s, 3H), 3.26-2.98 (m, 11H), 2.38 (dd, J=14.8, 14.4 Hz, 1H), 2.29-2.25 (m, 1H), 1.80-1.73 (m, 2H), 1.67-1.64 (m, 1H), 1.02 (t, J=7.2 Hz, 3H); MS (ESI) m/z 516.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.04, 7.02 (s, 1H total), 4.73, 4.60 (d, J=12.8 Hz, 1H total), 4.51, 4.44 (d, J=13.2 Hz, 1H total), 4.11 (s, 1H), 3.78, 3.76 (s, 3H total), 3.24-2.98 (m, 11H), 2.52-2.44 (m, 1H), 2.41-2.30 (m, 1H), 2.27-2.20 (m, 2H), 1.98-1.94 (m, 1H), 1.88-1.82 (m, 1H), 1.80-1.60 (m, 2H), 1.31-1.29 (m, 1H), 1.22-1.18 (m, 1H), 1.12-1.08 (m, 1H), 1.00-0.92 (m, 1H), 0.90-0.83 (m, 1H); MS (ESI) m/z 568.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.04 (s, 1H), 4.41 (d, J=13.6 Hz, 1H), 4.28 (d, J=13.6, Hz, 1H), 4.09 (s, 1H), 3.74 (s, 3H), 3.63-3.58 (m, 4H), 3.24-2.96 (m, 13H), 2.38 (dd, J=14.8, 14.0 Hz, 1H), 2.25-2.20 (m, 1H), 2.18-2.10 (m, 4H), 1.66-1.60 (m, 1H); MS (ESI) m/z 571.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.12 (s, 1H), 4.60-4.53 (m, 1H), 4.42-4.37 (m, 1H), 4.11 (s, 1H), 3.78-3.62 (m, 9H), 3.24-2.90 (m, 14H), 2.39 (dd, J=14.8, 14.0 Hz, 1H), 2.27-2.23 (m, 1H), 2.20-2.11 (m, 4H), 1.69-1.60 (m, 1H); MS (ESI) m/z 585.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.14 (s, 1H), 4.58-4.52 (m, 1H), 4.41-4.38 (m, 1H), 4.12 (s, 1H), 3.76-3.64 (m, 9H), 3.26-2.97 (m, 13H), 2.39 (dd, J=14.8, 14.4 Hz, 1H), 2.27-2.24 (m, 1H), 2.20-2.11 (m, 4H), 1.69-1.60 (m, 1H), 1.43 (t, J=6.8 Hz, 3H); MS (ESI) m/z 599.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.36-7.28 (m, 5H), 7.05, 6.98 (s, 1H total), 4.39-4.34 (m, 1H), 4.26-4.20 (m, 1H), 4.11 (s, 1H), 3.78, 3.73 (s, 3H total), 3.25-2.98 (m, 13H), 2.39 (dd, J=15.2, 14.4 Hz, 1H), 2.27-2.24 (m, 1H), 1.72-1.62 (m, 1H); MS (ESI) m/z 578.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.30-7.26 (m, 5H), 7.04 (s, 1H), 4.66, 4.16 (d, J=12.8 Hz, 1H total), 4.45-4.38 (m, 1H), 4.13 (s, 1H), 3.74, 3.72 (s, 3H total), 3.45-3.34 (m, 2H), 3.20-2.97 (m, 11H), 2.89, 2.86 (s, 3H total), 2.42-2.33 (m, 1H), 2.29-2.19 (m, 1H), 1.65-1.58 (m, 1H); MS (ESI) m/z 592.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.33-7.24 (m, 5H), 7.06 (s, 1H), 4.59-4.54 (m, 1H), 4.36 (d, J=13.6 Hz, 1H), 4.13 (s, 1H), 3.77-3.74 (m, 3H), 3.36-3.34 (m, 2H), 3.22-2.98 (m, 13H), 2.45-2.38 (m, 1H), 2.30-2.15 (m, 1H), 1.71-1.59 (m, 1H), 1.43-1.40 (m, 3H); MS (ESI) m/z 606.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.02, 7.01 (s, 1H total), 4.58, 4.21 (d, J=13.2 Hz, 1H total), 4.45, 4.02 (d, J=12.8 Hz, 1H total), 4.12 (s, 1H), 3.74-3.73 (m, 3H), 3.24-2.97 (m, 10H), 2.73 (s, 3H), 2.45-2.37 (m, 1H), 2.33-2.24 (m, 2H), 2.15-2.13 (m, 1H), 1.88-1.82 (m, 4H), 1.74-1.61 (m, 3H); MS (ESI) m/z 556.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.10-7.01 (m, 1H), 4.48-4.19 (m, 2H), 4.12 (s, 1H), 3.80-3.74 (m, 3H), 3.26-2.97 (m, 12H), 2.45-2.35 (m, 1H), 2.29-2.20 (m, 2H), 2.15-2.10 (m, 1H), 1.87-1.80 (m, 4H), 1.73-1.64 (m, 3H), 1.34 (t, J=7.2 Hz, 3H); MS (ESI) m/z 570.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.07-7.00 (m, 1H), 4.56-4.35 (m, 2H), 4.12 (s, 1H), 3.93-3.84 (m, 1H), 3.79, 3.73 (s, 3H total), 3.16-2.61 (m, 13H), 2.46-2.31 (m, 1H), 2.25-2.12 (m, 1H), 1.68-1.64 (m, 1H), 1.43-0.96 (m, 9H); MS (ESI) m/z 558.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.08, 7.00 (s, 1H total), 4.63, 4.55 (dd, J=13.6 Hz, 1H total), 4.39-4.33 (m, 1H), 4.12 (s, 1H), 3.85-3.73 (m, 3H), 3.36-3.33 (m, 1H), 3.22-2.96 (m, 11H), 2.46-2.38 (m, 1H), 2.25-2.15 (m, 2H), 1.70-1.61 (m, 1H), 1.46-0.94 (m, 12H); MS (ESI) m/z 572.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.06, 7.99 (s, 1H total), 4.31 (d, J=12.8 Hz, 1H), 4.18 (d, J=13.6 Hz, 1H), 4.13 (s, 1H), 3.80, 3.75 (s, 3H total), 3.27-2.99 (m, 10H), 2.42-2.35 (m, 1H), 2.30-2.27 (m, 1H), 2.20-2.16 (m, 2H), 1.92-1.89 (m, 2H), 1.74-1.65 (m, 2H), 1.43-1.36 (m, 4H), 1.29-1.24 (m, 1H); MS (ESI) m/z 556.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.02, 7.01 (s, 1H total), 4.61, 4.47 (d, J=12.8 Hz, 1H total), 4.13 (s, 1H), 3.96 (d, J=12.8 Hz, 1H), 3.77, 3.74 (s, 3H total), 3.24-2.98 (m, 10H), 2.74 (s, 3H), 2.46-2.37 (m, 1H), 2.32-2.29 (m, 1H), 2.18-2.10 (m, 2H), 2.00-1.96 (m, 2H), 1.74-1.51 (m, 4H), 1.43-1.32 (m, 3H); MS (ESI) m/z 570.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.07, 7.01 (s, 1H total), 4.59, 4.43 (d, J=13.6 Hz, 1H total), 4.29, 4.09 (d, J=13.6 Hz, 1H total), 4.14 (s, 1H), 3.77, 3.75 (s, 3H total), 3.28-2.99 (m, 12H), 2.46-2.40 (m, 1H), 2.36-2.31 (m, 1H), 2.16-2.10 (m, 2H), 1.99-1.96 (m, 2H), 1.74-1.58 (m, 4H), 1.41-1.23 (m, 6H); MS (ESI) m/z 584.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.98 (s, 1H), 4.31 (d, J=13.6 Hz, 1H), 4.18 (d, J=13.2 Hz, 1H), 4.12 (s, 1H), 3.75 (s, 3H), 3.27-2.99 (m, 11H), 2.40 (dd, J=15.2, 14.8 Hz, 1H), 2.28-2.25 (m, 1H), 1.72-1.63 (m, 1H), 1.36 (t, J=7.2 Hz, 3H); MS (ESI) m/z 502.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.44 (d, J=13.6 Hz, 1H), 4.24 (d, J=13.2 Hz, 1H), 4.11 (s, 1H), 3.75 (s, 3H), 3.25-2.97 (m, 13H), 2.41 (dd, J=15.2, 14.8 Hz, 1H), 2.26 (ddd, J=13.6, 4.8, 2.8 Hz, 1H), 1.68 (ddd, J=13.6, 13.6, 13.6 Hz, 1H), 1.39-1.36 (m, 6H); MS (ESI) m/z 530.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.06, 6.99 (s, 1H total), 4.35 (d, J=13.2 Hz, 1H), 4.25-4.18 (m, 1H), 4.09 (s, 1H), 3.79, 3.74 (s, 3H total), 3.23-2.95 (m, 11H), 2.38 (dd, J=14.8, 14.4 Hz, 1H), 2.26-2.14 (m, 1H), 1.90-1.80 (m, 6H), 1.65-1.56 (m, 3H), 0.79 (t, J=7.2 Hz, 3H); MS (ESI) m/z 570.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.47, 4.10 (d, J=12.8 Hz, 1H total), 4.33 (dd, J=12.8, 12.8 Hz, 1H), 4.10 (s, 1H), 3.75, 3.71 (s, 3H total), 3.24-2.95 (m, 11H), 2.83, 2.80 (m, 3H total), 2.40 (dd, J=14.8, 14.8 Hz, 1H), 2.25-2.23 (m, 1H), 2.03-1.83 (m, 6H), 1.80-1.70 (m, 2H), 1.69-1.59 (m, 1H), 0.88-0.80 (m, 3H); MS (ESI) m/z 584.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.42, 4.35 (d, J=13.2 Hz, 1H total), 4.20 (d, J=13.2 Hz, 1H), 4.09 (s, 1H), 3.74, 3.70 (s, 3H total), 3.23-2.94 (m, 13H), 2.37 (dd, J=14.4, 13.2 Hz, 1H), 2.25-2.22 (m, 1H), 1.84-1.85 (m, 6H), 1.75-1.71 (m, 2H), 1.69-1.58 (m, 1H), 1.43-1.37 (m, 3H), 0.88-0.80 (m, 3H); MS (ESI) m/z 598.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.67, 4.25 (d, J=13.2 Hz, 1H total), 4.40 (dd, J=14.0, 14.0 Hz, 1H), 4.09 (s, 1H), 3.76, 3.72 (s, 3H total), 3.46-3.41 (m, 1H), 3.21-2.93 (m, 12H), 2.38 (dd, J=14.4, 14.4 Hz, 1H), 2.25-2.22 (m, 1H), 1.98-1.82 (m, 6H), 1.76-1.71 (m, 2H), 1.69-1.61 (m, 1H), 1.30-1.20 (m, 1H), 0.86-0.75 (m, 5H), 0.44 (dd, J=16.0, 4.4 Hz, 2H); MS (ESI) m/z 624.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.04, 6.97 (s, 1H total), 4.22 (d, J=12.4 Hz, 1H), 4.10-4.04 (m, 2H), 3.77, 3.72 (s, 3H total), 3.23-2.94 (m, 9H), 2.35 (dd, J=14.8, 14.8 Hz, 1H), 2.25-2.22 (m, 1H), 1.78 (s, 2H), 1.67-1.58 (m, 1H), 1.52 (s, 6H), 1.08 (s, 9H); MS (ESI) m/z 586.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.05, 6.98 (s, 1H total), 4.71, 4.60 (d, J=12.8 Hz, 1H total), 4.10 (m, 1H), 3.93-3.87 (m, 1H), 3.77-3.71 (m, 3H), 3.21-2.95 (m, 9H), 2.66 (s, 3H), 2.41 (dd, J=14.4, 14.4 Hz, 1H), 2.33-2.23 (m, 1H), 2.00-1.94 (m, 1H), 1.81 (d, J=14.0 Hz, 1H), 1.66-1.57 (m, 7H), 1.12 (s, 9H); MS (ESI) m/z 600.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.09, 7.08 (s, 1H total), 4.70, 4.55 (d, J=13.2 Hz, 1H total), 4.28, 4.13 (d, J=13.2 Hz, t, J=6.4 Hz, 2H total), 3.77, 3.76 (s, 3H total), 3.54-3.47 (m, 1H), 3.27-2.99 (m, 10H), 2.42 (dd, J=14.4, 14.4 Hz, 1H), 2.30-2.27 (m, 1H), 2.00-1.88 (m, 2H), 1.72-1.66 (m, 7H), 1.15 (s, 9H), 1.08 (t, J=7.2 Hz, 3H); MS (ESI) m/z 614.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.10, 7.06 (s, 1H total), 4.70, 4.55 (d, J=13.2 Hz, 1H total), 4.28, 4.13 (d, J=13.2 Hz, t, J=6.4 Hz, 2H total), 3.77, 3.76 (s, 3H total), 3.54-3.47 (m, 1H), 3.27-2.99 (m, 10H), 2.42 (dd, J=14.4, 14.4 Hz, 1H), 2.30-2.27 (m, 1H), 2.00-1.88 (m, 2H), 1.72-1.66 (m, 7H), 1.15 (s, 9H), 1.08 (t, J=7.2 Hz, 3H); MS (ESI) m/z 640.4 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.01, 6.99 (s, 1H total), 4.57-4.36 (m, 1H), 4.33-4.12 (m, 1H), 4.15, 4.13 (s, 1H total), 3.77, 3.73 (s, 3H total), 3.58-3.40 (m, 1H), 3.24-2.96 (m, 11H), 2.44-2.37 (m, 1H), 2.28-2.24 (m, 1H), 2.04-1.84 (m, 2H), 1.70-1.60 (m, 1H), 1.47-1.36 (m, 3H), 1.06-0.96 (m, 4H), 0.76-0.68 (m, 2H), 0.42-0.31 (m, 2H); MS (ESI) m/z 584.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.02, 7.01 (s, 1H total), 4.56-4.39 (m, 1H), 4.12 (s, 1H), 4.02-3.93 (m, 1H), 3.78-3.74 (m, 3H), 3.35-3.32 (m, 1H), 3.26-2.98 (m, 9H), 2.74, 2.73 (s, 3H total), 2.46-2.33 (m, 1H), 2.28-2.25 (m, 1H), 1.99-1.92 (m, 2H), 1.82-1.60 (m, 1H), 1.47-1.40 (m, 3H), 1.09-1.00 (m, 3H); MS (ESI) m/z 544.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.03-7.00 (m, 1H), 4.53-4.49 (m, 1H), 4.37-4.34 (m, 1H), 4.12 (s, 1H), 3.77-3.73 (m, 3H), 3.42-3.34 (m, 1H), 3.26-2.97 (m, 11H), 2.42 (dd, J=14.4, 14.4 Hz, 1H), 2.28-2.25 (m, 1H), 1.97-1.92 (m, 1H), 1.72-1.61 (m, 2H), 1.48-1.46 (m, 6H), 1.08-0.98 (m, 3H); MS (ESI) m/z 558.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.97 (s, 1H), 4.12-4.09 (m, 2H), 3.85 (d, J=13.2 Hz, 1H), 3.74 (s, 3H), 3.25-2.96 (m, 9H), 2.46-4.38 (m, 3H), 2.27-2.24 (m, 1H), 2.12-1.93 (m, 4H), 1.69-1.60 (m, 4H); MS (ESI) m/z 542.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.03 (s, 1H), 4.31, 4.03 (d, J=12.4 Hz, 1H total), 4.21, 3.88 (d, J=13.2 Hz, 1H total), 4.13 (s, 1H), 3.78, 3.73 (s, 3H total), 3.25-2.98 (m, 9H), 2.66-2.61 (m, 3H), 2.56-2.29 (m, 2H), 2.26-2.17 (m, 2H), 2.05-2.00 (m, 1H), 1.94-1.88 (m, 2H), 1.71-1.60 (m, 4H); MS (ESI) m/z 556.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.07, 7.06 (s, 1H total), 4.50, 4.16 (d, J=13.6 Hz, 1H total), 4.31-4.29 (m, 1H), 4.12 (s, 1H), 3.76, 3.75 (s, 3H total), 3.26-2.96 (m, 11H), 2.61-2.56 (m, 1H), 2.43-2.25 (m, 3H), 2.09-2.04 (m, 1H), 1.95-1.70 (m, 3H), 1.70-1.61 (m, 4H), 0.93-0.90 (m, 1H), 0.71-0.67 (m, 2H), 0.37-0.34 (m, 2H); MS (ESI) m/z 596.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.96 (s, 1H), 4.20-4.12 (m, 2H), 4.06 (d, J=13.6 Hz, 1H), 3.82-3.79 (m, 1H), 3.74 (s, 3H), 3.26-2.98 (m, 9H), 2.42-2.20 (m, 6H), 1.96-1.89 (m, 2H), 1.71-1.62 (m, 1H); MS (ESI) m/z 528.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.04, 7.03 (s, 1H total), 4.36-4.32 (m, 1H), 4.16-4.13 (m, 1H), 4.02-3.98 (m, 1H), 3.81, 3.76 (s, 3H total), 3.26-2.98 (m, 12H), 2.66-2.02 (m, 6H), 1.84-1.60 (m, 3H), 1.16-1.14 (m, 1H), 0.80-0.77 (m, 2H), 0.41-0.38 (m, 2H); MS (ESI) m/z 582.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.14, 7.06 (s, 1H total), 4.55-4.78 (m, 1H), 4.33-4.29 (m, 1H), 4.12, 3.98 (s, 1H total), 3.82-3.74 (m, 3H), 3.24-2.98 (m, 13H), 2.38 (dd, J=12.8, 12.8 Hz, 1H), 2.29-2.26 (m, 1H), 2.18-2.15 (m, 1H), 1.72-1.62 (m, 1H), 1.46-1.42 (m, 3H), 1.06-1.02 (m, 9H); MS (ESI) m/z 572.2 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.14-7.06 (m, 1H), 4.76, 4.32 (d, J=13.6 Hz, 1H total), 4.61-4.47 (m, 1H), 4.12, 3.89 (s, 1H total), 3.82-3.78 (m, 3H), 3.25-2.98 (m, 13H), 2.42-2.15 (m, 2H), 1.72-1.63 (m, 1H), 1.32-2.28 (m, 1H), 1.04, 1.00 (s, 9H total), 0.87-0.82 (m, 2H), 0.53-0.48 (m, 2H); MS (ESI) m/z 598.3 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.70-7.51 (m, 5H), 6.98-6.86 (m, 1H), 4.62-4.35 (m, 2H), 4.13 (s, 1H), 4.10-3.78 (m, 1H), 3.51-3.57 (m, 3H), 3.22-2.87 (m, 9H), 2.80, 2.79, 2.68, 2.67 (s, 3H total), 2.40-2.23 (m, 2H), 1.88-1.80 (m, 3H), 1.68-1.58 (m, 1H); MS (ESI) m/z 592.0 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.67-7.52 (m, 5H), 6.95, 6.90, 6.81, 6.74 (s, 1H total), 4.68-4.61 (m, 1H), 4.48-4.17 (m, 1.5H), 4.13, 4.11 (s, 1H total), 4.10-4.00 (m, 0.5H), 3.48, 3.45, 3.41, 3.39 (s, 3H total), 3.21-2.88 (m, 11H), 2.39-2.22 (m, 2H), 1.85-1.76 (m, 3H), 1.68-1.58 (m, 1H), 1.45-1.34 (m, 3H); MS (ESI) m/z 606.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.02 (s, 1H), 4.67-4.39 (m, 3H), 4.12 (s, 1H), 3.72 (s, 3H), 3.26-3.20 (m, 2H), 3.05-2.89 (m, 9H), 2.49-2.40 (m, 1H), 2.38-2.32 (m, 1H), 2.29-2.22 (m, 1H), 2.17-2.11 (m, 2H), 2.07-2.00 (m, 1H), 1.70-1.60 (m, 1H); MS (ESI) m/z 596.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.82-7.55 (m, 5H), 6.89 (s, 1H), 4.22, 3.95 (d, J=−12.4 Hz, total 1H), 4.13 (s, 1H), 3.11-3.00 (m, 11H), 2.83, 2.80 (s, total 3H), 2.37-2.25 (m, 2H), 1.98-1.88 (m, 6H), 1.69-1.60 (m, 1H); MS (ESI) m/z 606.1 (M+H).

S24-9-182:

MS (ESI) m/z 592.3 (M+H).

Example 25. Synthesis of Compounds Via Scheme 25

The following compounds were prepared according to Scheme 25.

Compound S15-5 (20 g, 62.5 mmol, 1.0 equiv), 2, 4, 6 Trivinylcyclotriboroxane-pyridine complex (7.8 g, 31.25 mmol, 0.50 equiv), Pd(PPh₃)₄ (2.2 g, 1.88 mmol, 0.030 equiv) and K₂CO₃ (17.25 g, 125 mmol, 2.0 equiv) was added to vessel in 1.4 dioxane:H₂O (3:1, V:V). The mixture was bubbled with N₂ to remove O₂ for 6 times. The mixture was heated to reflux for 19 h. The mixture was concentrated. The residue partitioned between EA and water. The organic layer was dried over Na₂SO₄ and evaporated to dryness. The crude compound was purified by column chromatography on silica gel elute with (PE:EA=200:1→100:1→50:1), yielded (88.3%) 14.8 g compound S25-1 as a light yellow solid. ¹H NMR (400 MHz, CDCl₃) δ 7.38-7.34 (m, 2H), 7.27-7.16 (m, 3H), 6.83-6.76 (m, 2H), 6.65-6.60 (m, 1H), 5.72 (d, J=17.6 Hz, 1H), 5.25 (d, J=11.2 Hz, 1H), 3.83 (s, 3H), 2.38 (s, 3H); MS (ESI) m/z 269.1 (M+H).

An ozone-enriched steam of oxygen was bubbled through a cold (−78 C) solution of compound S25-1 (21 g, 78.3 mmol, 1.0 equiv) in anhydrous CH₂Cl₂ until it turned light blue. The reaction was followed by TLC. The solution was purged with argon at −78 C for 10 min to remove the excess O₃. CH₃SCH₃ (50 mL) was added into the reaction mixture and stirred for 5 hour from −78 C to 25 C. The reaction was concentrated. The crude compound was purified by column chromatography on silica gel elute with (PE:EA=100:1→50:→30:1) to yield (61.6%) 13 g compound S25-2 as a light yellow solid. ¹H NMR (400 MHz, CDCl₃) δ 9.97 (s, 1H), 7.46-7.41 (m, 2H), 7.36-7.22 (m, 5H), 3.92 (s, 3H), 2.51 (s, 3H); MS (ESI) m/z 271.1 (M+H).

Compound S25-3 (1.8 g, 6.62 mmol, 1 equiv) was dissolved in HOAc. Bromine (1.6 mL, 26.5 mmol, 4 equiv) was added dropwise into the solution. The reaction mixture was stirred for 1 hour at rt. The mixture was concentrated. The residue was extracted with EA and a saturated NaHCO₃. The organic layer was washed with brine and water in return, dried over Na₂SO₄ and concentrated to dryness. To afford 1.9 g compound S25-3 as a light yellow solid.

BBr₃ (4.9 g, 1.9 mL, 19.5 mmol, 1.5 equiv) was added to a CH₂Cl₂ solution (30 mL) of S25-3 (3.5 g, 13.0 mmol, 1.0 equiv) at −78 C. The reaction was stirred from −78° C. to 25° C. for 1.5 h, quenched with saturated NaHCO₃ and the reaction mixture was extracted with EtOAc. The combined EtOAc extracts were dried (Na₂SO₄) and concentrated to yield 3.3 g of crude S25-4.

K₂CO₃ (3.6 g, 26.0 mmol, 2.0 equiv) and BnBr (4.2 g, 26.0 mmol, 2.0 equiv) were added to a solution of compound S25-4 (3.3 g, 13.0 mmol, 1.0 equiv) in DMF (15 mL). The reaction mixture was stirred at rt for 2 h. The reaction mixture was filtered and washed with EtOAc. Water (150 mL) was added into it and extracted with EtOAc. The organic layer was dried over Na₂SO₄ and concentrated. The crude compound was purified by column chromatography on silica gel elute with (PE:EA=100:1→50:1), yielded (61.7% for 3 steps) 3.5 g compound S25-5 as a light yellow solid. ¹H NMR (400 MHz, CDCl₃) δ 10.43 (s, 1H), 7.46-7.30 (m, 9H), 7.08-7.05 (m, 2H), 5.17 (s, 2H), 2.52 (s, 3H); MS (ESI) m/z 425.1 (M+H).

Compound S25-5 (5 g, 11.8 mmol, 1.0 equiv) in anhydrous DMF was added CH₃O₂CCF₂SO₂F (11.3 g, 59 mmol, 5.0 equiv) and CuI (4.5 g, 23.6 mmol, 2.0 equiv) in obturator. The reaction was heated to 100 C for 20 h. The mixture was filtered and washed with EA. The solution was concentrated and extracted with EA and water. The organic layer was dried over Na₂SO₄ and concentrated to the crude compound S25-6 as brown oil (7 g). ¹H NMR (400 MHz, CDCl₃) δ 10.35-10.32 (m, 1H), 7.40-7.28 (m, 9H), 7.02-6.83 (m, 2H), 5.17 (s, 2H), 2.55-2.51 (m, 3H); MS (ESI) m/z 415.1 (M+H).

Compound S25-6 (7 g crude, 11.8 mmol, 1.0 equiv) in MeOH was added HC(OMe)₃ (3.6 g, 35.4 mmol, 3.0 equiv) and TsOH (0.23 g, 1.18 mmol, 0.1 equiv). The reaction was heated to reflux for 18 h. The mixture was concentrated. The residue was extracted with EA and water. The organic layer was dried over Na₂SO₄ and concentrated. The crude compound was purified by column chromatography on silica gel elute with (PE:EA=100:1→50:1) to yield (90.6% for 2 steps) 4.9 g compound S25-7 as a light yellow oil. ¹H NMR (400 MHz, CDCl₃) δ 7.45-7.30 (m, 9H), 7.08-6.90 (m, 2H), 5.60 (s, 1H), 5.20 (s, 2H), 3.73 (s, 6H), 2.56-2.51 (m, 3H); MS (ESI) m/z 461.1 (M+H).

To diisopropylamine (0.414 mL, 2.5 mmol, 5.0 equiv) in THF at −78° C. was added nBuLi (1.04 mL, 2.50 M/hexane, 2.5 mmol, 5.0 equiv) and TMEDA (1.04 mL, 2.5 mmol, 5.0 equiv) at −78° C. dropwise. The reaction was stirred at −78° C. for 30 min. Compound S25-7 (460 mg, 1.25 mmol, 2.5 equiv) in THF was added to the reaction mixture dropwise at −78 C. The resulting deep-red solution was stirred at −78° C. 15 min and added with the enone (240 mg 0.50 mmol, 1.0 equiv) in THF. The deep-red solution was gradually warmed up with stirring from −78° C. to 0° C. over a period of 30 min. and quenched with aqueous saturated ammonium chloride (100 mL). The yellow-green mixture was extracted with EtOAc two times. The combined EtOAc extracts were dried (Na₂SO₄) and concentrated to yield the crude product. Flash column chromatography on silica gel with 0%, 5%, 10%, and 20% EtOAc/hexane sequentially yielded the desired product S25-8 as a light-yellow solid (380 mg, 89%). ¹H NMR (400 MHz, CDCl₃) δ 15.65 (s, 1H), 7.46-7.22 (m, 11H), 5.49 (s, 1H), 5.30 (s, 2H), 5.25 (s, 2H), 3.91 (d, J=10.8 Hz, 1H), 3.29 (s, 3H), 3.20 (s, 3H), 2.85-2.62 (m, 2H), 2.53-2.35 (m, 9H), 2.10-2.04 (m, 1H), 0.88 (s, 9H), 0.20 (s, 3H), 0.09 (s, 3H); MS (ESI) m/z 849.1 (M+H).

Compound S25-8 (0.3 g, 0.354 mmol) was dissolved in DCM (3 mL) and aqueous TFA (3 mL) was added dropwise. The yellow solution was stirred at rt for 1 h. The reaction was followed by LC-MS. The solution was concentrated. The residue was extracted with EA and water. The combined EtOAc extracts were dried (Na₂SO₄) and concentrated to yield the crude product S25-9.

Compound S25-9 (50 mg crude, 0.059 mmol, 1.0 equiv) was dissolved in 1,2-dichloroethane (1.0 mL). Pyrroliding (0.295 mmol, 5.0 equiv) and acetic acid (20 μL, 0.36 mmol, 6.0 equiv) were added. After stirring at rt for 1 h, sodium triacetoxyborohydride (37 mg, 0.177 mmol, 3.0 equiv) was added. Stirring was continued overnight. The reaction mixture was poured into saturated aqueous NaHCO₃ and extracted three times with dichloromethane. The combined organic extracts were washed with brine, dried over sodium sulfate, and concentrated to give the crude intermediate S25-10-1, which was used directly in the next step without further purification.

Compound S25-10-1 (50 mg, crude, 0.059 mmol, 1 equiv) was dissolved in THF (10 mL) and aqueous HF (40%, 10 mL) was added dropwise. The yellow solution was stirred at rt for 1 h. The resulting deep-red solution was slowly added into an aqueous K₂HPO₄ solution with stirring. The pH of the mixture was adjusted by aqueous K₂HPO₄ solution to about 8. The yellow mixture was extracted with EtOAc two times. The combined EtOAc extracts were dried (Na₂SO₄) and concentrated to yield the crude intermediate.

The above crude intermediate (50 mg, crude, 0.059 mmol, 1 equiv) was dissolved in HPLC grade MeOH (10 mL). HCl/MeOH (1.0 mL, 4N) and 10% Pd—C (50 mg, 0.046 mmol, 0.78 equiv) were added. The mixture was purged with hydrogen by bubbling hydrogen through with gentle stirring for 5 min. The reaction was then vigorously stirred under hydrogen balloon at rt for 1 hr. LC-MS analysis indicated complete reaction. The catalyst was filtered and concentrated to yield the crude product as a deep-yellow solid. The crude compound was purified by prep-HPLC on a Polymerx column to yield the desired product S25-11-1 as a yellow solid after freeze-drying (7 mg, 21%). ¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.71 (d, J=14.0 Hz, 1H), 4.49 (d, J=14.0 Hz, 1H), 4.14 (s, 1H), 3.74-3.59 (m, 2H), 3.30-2.96 (m, 11H), 2.69-2.57 (m, 1H), 2.26-2.01 (m, 5H), 1.69-1.59 (m, 1H); MS (ESI) m/z 566.1 (M+H).

The following final compounds were prepared from S25-9 by reductive amination with various amines, followed by aqueous HF treatment and hydrogenation under similar conditions described for S25-11-1.

S25-11-2

¹H NMR (400 MHz, CD₃OD) δ 7.17 (s, 1H), 4.43 (d, J=14.0 Hz, 1H), 4.32 (d, J=14.0 Hz, 1H), 4.13 (s, 1H), 3.16 (t, J=8.4 Hz, 2H), 3.09-2.96 (m, 9H), 2.65-2.62 (m, 1H), 2.26-2.22 (m, 1H), 1.77-1.63 (m, 3H), 1.49-1.43 (m, 2H), 1.02 (t, J=7.2 Hz, 3H); MS (ESI) m/z 568.2 (M+H).

S25-11-3:

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.79-4.67 (m, 1H), 4.45-4.29 (m, 1H), 4.15 (s, 1H), 3.26-2.97 (m, 11H), 2.90-2.81 (m, 3H), 2.69-2.57 (m, 1H), 2.28-2.24 (m, 1H), 1.83-1.75 (m, 2H), 1.69-1.60 (m, 1H), 1.47-1.38 (m, 2H), 1.00 (t, J=7.2 Hz, 3H); MS (ESI) m/z 582.1 (M+H).

S25-11-4:

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.65-4.55 (m, 1H), 4.53-4.45 (m, 1H), 4.12 (s, 1H), 3.37-3.33 (m, 2H), 3.26-2.95 (m, 11H), 2.68-2.57 (m, 1H), 2.26-2.20 (m, 1H), 1.82-1.60 (m, 3H), 1.44-1.35 (m, 5H), 1.01 (t, J=7.2 Hz, 3H); MS (ESI) m/z 596.3 (M+H).

S25-11-5:

¹H NMR (400 MHz, CD₃OD) δ 7.30-7.21 (m, 5H), 4.51 (d, J=13.6 Hz, 1H), 4.40 (d, J=14.0 Hz, 1H), 4.27-4.24 (m, 1H), 4.12 (s, 1H), 3.66-3.47 (m, 2H), 3.24-2.95 (m, 11H), 2.65-2.61 (m, 1H), 2.25-2.22 (m, 1H), 1.65-1.60 (m, 1H); MS (ESI) m/z 628.2 (M+H).

S25-11-6:

¹H NMR (400 MHz, CD₃OD) δ 7.14 (s, 1H), 4.29 (d, J=14.0 Hz, 1H), 4.17 (d, J=14.8 Hz, 1H), 4.13 (s, 1H), 3.93-3.89 (m, 1H), 3.09-2.95 (m, 9H), 2.65-2.60 (m, 1H), 2.42-2.22 (m, 5H), 1.98-1.89 (m, 2H), 1.65-1.60 (m, 1H); MS (ESI) m/z 566.2 (M+H).

S25-11-7:

¹H NMR (400 MHz, CD₃OD) δ 7.16 (s, 1H), 4.44 (d, J=14.0 Hz, 1H), 4.35 (d, J=14.0 Hz, 1H), 4.12 (s, 1H), 3.23-2.96 (m, 11H), 2.66-2.62 (m, 1H), 2.25-2.21 (m, 1H), 1.70-1.62 (m, 3H), 1.05 (s, 9H); MS (ESI) m/z 596.0 (M+H).

S25-11-8:

¹H NMR (400 MHz, CD₃OD) δ 7.16 (s, 1H), 4.50 (d, J=15.2 Hz, 1H), 4.38 (d, J=15.2 Hz, 1H), 4.13 (s, 1H), 3.78-3.68 (m, 3H), 3.37-3.31 (m, 2H), 3.26-3.25 (m, 1H), 3.10-2.92 (m, 8H), 2.66-2.61 (m, 1H), 2.26-2.22 (m, 1H), 1.69-1.63 (m, 1H), 1.21 (d, J=6.4 Hz, 6H); MS (ESI) m/z 598.1 (M+H).

S25-11-9:

¹H NMR (400 MHz, CD₃OD) δ 7.08 (s, 1H), 4.35 (d, J=14.4 Hz, 1H), 4.23 (d, J=13.6 Hz, 1H), 4.03 (s, 1H), 3.09 (t, J=8.4 Hz, 2H), 2.95-2.86 (m, 9H), 2.52-2.48 (m, 1H), 2.16-2.13 (m, 1H), 1.64-1.44 (m, 4H), 0.90 (d, J=6.4 Hz, 6H); MS (ESI) m/z 582.1 (M+H).

S25-11-10:

¹H NMR (400 MHz, CD₃OD) δ 7.15 (s, 1H), 4.36-4.29 (m, 2H), 4.04 (s, 1H), 3.03-2.86 (m, 11H), 2.56-2.49 (m, 1H), 2.17-2.12 (m, 1H), 1.60-1.50 (m, 1H), 1.00 (s, 9H); MS (ESI) m/z 582.2 (M+H).

S25-11-11:

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.56 (d, J=14.0 Hz, 1H), 4.39 (d, J=14.0 Hz, 1H), 4.13 (s, 1H), 3.19-2.90 (m, 10H), 2.69-2.59 (m, 1H), 2.25-2.22 (m, 1H), 1.69-1.59 (m, 1H), 1.39 (d, J=8.8 Hz, 3H), 1.05 (s, 9H); MS (ESI) m/z 596.2 (M+H).

S25-11-12:

¹H NMR (400 MHz, CD₃OD) δ 7.22, 7.20 (s, 1H total), 4.43-4.26 (m, 2H), 4.13 (s, 1H), 3.64-3.62 (m, 1H), 3.25-2.95 (m, 9H), 2.67-2.57 (m, 2H), 2.35-2.11 (m, 3H), 1.68-1.43 (m, 7H), 1.18-1.12 (m, 1H); MS (ESI) m/z 606.3 (M+H).

S25-11-13:

¹H NMR (400 MHz, CD₃OD) δ 7.18 (s, 1H), 4.40 (d, J=14.0 Hz, 1H), 4.31 (d, J=14.0 Hz, 1H), 4.14 (s, 1H), 3.25-2.98 (m, 11H), 2.78-2.55 (m, 2H), 2.26-2.18 (m, 3H), 2.08-1.85 (m, 4H), 1.70-1.58 (m, 1H); MS (ESI) m/z 580.3 (M+H).

S25-11-14:

¹H NMR (400 MHz, CD₃OD) δ 7.05 (s, 1H), 4.32 (d, J=14.0 Hz, 1H), 4.20 (d, J=14.4 Hz, 1H), 4.00 (s, 1H), 3.15-2.82 (m, 11H), 2.51-2.43 (m, 1H), 2.13-2.09 (m, 1H), 1.55-1.45 (m, 1H), 1.10-0.99 (m, 1H), 0.65-0.58 (m, 2H), 0.35-0.27 (m, 2H); MS (ESI) m/z 566.1 (M+H).

S25-11-15:

¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.42-4.29 (m, 2H), 4.13 (s, 1H), 3.17-2.95 (m, 10H), 2.65-2.58 (m, 1H), 2.26-2.22 (m, 1H), 1.96-1.90 (m, 1H), 1.68-1.58 (m, 2H), 1.42-1.37 (m, 3H), 1.02 (t, J=7.2 Hz, 3H); MS (ESI) m/z 568.2 (M+H).

S25-11-16:

¹H NMR (400 MHz, CD₃OD) δ 7.33, 7.26 (s, 1H total), 4.57-4.29 (m, 2H), 4.13 (s, 1H), 3.62-3.50 (m, 1H), 3.22-2.84 (m, 12H), 2.68-2.55 (m, 1H), 2.26-2.22 (m, 1H), 2.07-1.83 (m, 2H), 1.68-1.44 (m, 3H), 1.12, 1.11 (s, 3H total), 1.04, 1.02 (s, 3H total); MS (ESI) m/z 608.3 (M+H).

S25-11-17:

¹H NMR (400 MHz, CD₃OD) δ 7.20 (s, 1H), 6.38 (tt, J=6.4, 54.0 Hz, 1H), 4.56 (dd, J=1.6, 14.0 Hz, 1H), 4.46 (dd, J=1.6, 14.8 Hz, 1H), 4.14 (s, 1H), 3.77-3.67 (m, 2H), 3.21-2.98 (m, 9H), 2.66-2.58 (m, 1H), 2.27-2.21 (m, 1H), 1.69-1.59 (m, 1H); MS (ESI) m/z 575.9 (M+H).

S25-11-18:

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.48-4.32 (m, 2H), 4.13 (s, 1H), 3.94-3.88 (m, 2H), 3.79-3.75 (m, 1H), 3.59-3.55 (m, 1H), 3.25-2.96 (m, 11H), 2.70-2.58 (m, 2H), 2.27-2.21 (m, 2H), 1.75-1.63 (m, 2H); MS (ESI) m/z 596.0

S25-11-19:

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.41 (d, J=14.4 Hz, 1H), 4.26 (d, J=14.8 Hz, 1H), 4.13 (s, 1H), 3.25-2.95 (m, 9H), 2.69-2.58 (m, 1H), 2.27-2.22 (m, 1H), 1.87-1.77 (m, 2H), 1.69-1.59 (m, 1H), 1.42 (s, 6H), 1.03 (t, J=7.2 Hz, 3H); MS (ESI) m/z 582.0 (M+H).

S25-11-20:

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.69 (d, J=14.8 Hz, 1H), 4.56 (d, J=14.8 Hz, 1H), 4.14 (s, 1H), 3.23-2.96 (m, 9H), 2.69-2.57 (m, 1H), 2.28-2.22 (m, 1H), 1.69-1.54 (m, 2H), 1.11-1.05 (m, 2H), 0.83-0.76 (m, 2H), 0.74-0.69 (m, 2H), 0.41-0.35 (m, 2H); MS (ESI) m/z 592.0 (M+H).

S25-11-21:

¹H NMR (400 MHz, CD₃OD) δ 7.41 (s, 4H), 7.32 (s, 1H), 4.78-4.67 (m, 6H), 4.13 (s, 1H), 3.22-2.95 (m, 9H), 2.67-2.58 (m, 1H), 2.25-2.22 (m, 1H), 1.68-1.59 (m, 1H); MS (ESI) m/z 614.0 (M+H).

S25-11-22:

¹H NMR (400 MHz, CD₃OD) δ 7.18 (s, 1H), 4.51 (d, J=13.6 Hz, 1H), 4.40 (d, J=13.2 Hz, 1H), 4.13 (s, 1H), 3.23-2.95 (m, 9H), 2.67-2.55 (m, 1H), 2.25-2.22 (m, 1H), 1.67-1.53 (m, 1H), 1.52 (s, 3H), 1.18-1.12 (m, 2H), 0.88-0.82 (m, 2H); MS (ESI) m/z 566.0 (M+H).

S25-11-23:

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.75-4.63 (m, 1H), 4.44-4.27 (m, 1H), 4.14 (s, 1H), 3.46-3.34 (m, 2H), 3.20-2.95 (m, 9H), 2.85 (d, J=14.8 Hz, 3H), 2.68-2.55 (m, 1H), 2.26-2.22 (m, 1H), 1.68-1.59 (m, 1H), 1.41 (t, J=7.2 Hz, 3H); MS (ESI) m/z 554.0 (M+H).

S25-11-24:

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.59 (d, J=14.0 Hz, 1H), 4.45 (d, J=14.4 Hz, 1H), 4.13 (s, 1H), 3.35-3.32 (m, 2H), 3.25-2.95 (m, 11H), 2.66-2.60 (m, 1H), 2.26-2.20 (m, 1H), 1.68-1.58 (m, 1H), 1.39-1.32 (m, 6H); MS (ESI) m/z 568.0 (M+H).

S25-11-25:

¹H NMR (400 MHz, CD₃OD) δ 7.20 (s, 1H), 4.64-4.59 (m, 1H), 4.51-4.45 (m, 1H), 4.12 (s, 1H), 3.35-3.32 (m, 2H), 3.20-2.95 (m, 11H), 2.69-2.59 (m, 1H), 2.25-2.22 (m, 1H), 1.82-1.60 (m, 3H), 1.40-1.33 (m, 3H), 1.05-0.96 (m, 3H); MS (ESI) m/z 582.2 (M+H).

S25-11-26:

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.61 (d, J=14.0 Hz, 1H), 4.45 (d, J=13.6 Hz, 1H), 4.14 (s, 1H), 3.18-2.93 (m, 15H), 2.69-2.58 (m, 1H), 2.27-2.23 (m, 1H), 1.67-1.58 (m, 1H); MS (ESI) m/z 540.1 (M+H).

S25-11-27:

¹H NMR (400 MHz, CD₃OD) δ 7.14 (s, 1H), 4.43 (d, J=14.4 Hz, 1H), 4.32 (d, J=14.4 Hz, 1H), 4.14 (s, 1H), 3.20-2.91 (m, 9H), 2.84 (s, 3H), 2.69-2.57 (m, 1H), 2.27-2.23 (m, 1H), 1.68-1.57 (m, 1H); MS (ESI) m/z 526.0 (M+H).

S25-11-18:

¹H NMR (400 MHz, CD₃OD) δ 7.48-7.30 (m, 5H), 7.08 (s, 1H), 4.35-4.24 (m, 2H), 4.15 (s, 1H), 3.45 (s, 2H), 3.21-2.96 (m, 9H), 2.69-2.53 (m, 1H), 2.26-2.22 (m, 1H), 1.67-1.57 (m, 1H), 1.48 (d, J=2.4 Hz, 6H); MS (ESI) m/z 644.1 (M+H).

S25-11-29:

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.71-4.61 (m, 1H), 4.41-4.24 (m, 1H), 4.15 (s, 1H), 3.25-2.98 (m, 11H), 2.96-2.81 (m, 4H), 2.69-2.59 (m, 1H), 2.26-2.20 (m, 3H), 2.10-2.01 (m, 1H), 1.99-1.90 (m, 1H), 1.69-1.59 (m, 1H); MS (ESI) m/z 594.0 (M+H).

S25-11-30:

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.80-4.75 (m, 1H), 4.42-4.24 (m, 1H), 4.12 (s, 1H), 3.22-2.89 (m, 14H), 2.68-2.60 (m, 1H), 2.25-2.21 (m, 1H), 1.69-1.62 (m, 1H), 1.27-1.10 (m, 1H), 0.83-0.80 (m, 2H), 0.50-0.47 (m, 2H); MS (ESI) m/z 580.1 (M+H).

S25-11-31:

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.62-4.43 (m, 2H), 4.11 (s, 1H), 3.20-2.87 (m, 13H), 2.69-2.59 (m, 1H), 2.25-2.20 (m, 1H), 1.69-1.60 (m, 1H), 1.41-1.33 (m, 3H), 1.28-1.10 (m, 1H), 0.83-0.75 (m, 2H), 0.46-0.38 (m, 2H); MS (ESI) m/z 594.1 (M+H).

S25-11-32:

¹H NMR (400 MHz, CD₃OD) δ 7.23, 7.22 (s, 1H total), 4.63 (d, J=14.4 Hz, 1H), 4.51-4.49 (m, 1H), 4.13 (s, 1H), 3.22-2.87 (m, 13H), 2.69-2.59 (m, 1H), 2.26-2.23 (m, 1H), 1.71-1.60 (m, 3H), 1.41-1.33 (m, 3H), 099, 0.97 (s, 9H total); MS (ESI) m/z 624.1 (M+H).

S25-11-33:

¹H NMR (400 MHz, CD₃OD) δ 7.50-7.28 (m, 5H), 7.07, 7.02 (s, 1H total), 4.46-4.43 (m, 1H), 4.33-4.19 (m, 1H), 4.13 (s, 1H), 3.78-3.51 (m, 2H), 3.22-2.86 (m, 9H), 2.66-2.57 (m, 4H), 2.24-2.21 (m, 1H), 1.66-1.47 (m, 1H), 1.45 (s, 6H); MS (ESI) m/z 658.0 (M+H).

S25-11-34:

¹H NMR (400 MHz, CD₃OD) δ 7.51-7.24 (m, 5H), 7.09, 6.94 (s, 1H total), 4.43-4.24 (m, 2H), 4.13 (s, 1H), 3.75-3.70 (m, 1H), 3.51-3.44 (m, 1H), 3.21-2.94 (m, 11H), 2.67-2.58 (m, 1H), 2.24-2.21 (m, 1H), 1.60-1.52 (m, 1H), 1.44 (t, J=7.2 Hz, 6H), 1.26-1.22 (m, 3H); MS (ESI) m/z 672.1 (M+H).

S25-11-35:

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.77-4.70 (m, 1H), 4.50, 38 (d, J=14.4 Hz, 1H, total), 4.13 (s, 1H), 3.89-3.68 (m, 3H), 3.52-3.40 (m, 2H), 3.21-2.88 (m, 12H), 2.64-2.61 (m, 1H), 2.26-2.22 (m, 1H), 1.67-1.58 (m, 1H), 1.20 (d, J=6.0 Hz, 6H); MS (ESI) m/z 612.4 (M+H).

S25-11-36:

¹H NMR (400 MHz, CD₃OD) δ 7.30, 7.26 (s, 1H total), 4.83-4.66 (m, 2H), 4.13 (s, 1H), 3.84-3.80 (m, 2H), 3.70-3.67 (m, 1H), 3.53-3.40 (m, 4H), 3.20-2.96 (m, 9H), 2.66-2.62 (m, 1H), 2.27-2.22 (m, 1H), 1.67-1.62 (m, 1H), 1.42-1.35 (m, 3H), 1.29-1.21 (m, 6H); MS (ESI) m/z 626.3 (M+H).

S25-11-37:

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.87-4.73 (m, 1H), 4.46-4.27 (m, 1H), 4.16 (s, 1H), 3.30-2.79 (m, 14H), 2.69-2.56 (m, 1H), 2.28-2.25 (m, 1H), 2.18-2.01 (m, 1H), 1.80-1.55 (m, 11H), 1.31-1.22 (m, 2H); MS (ESI) m/z 636.1 (M+H).

S25-11-38:

¹H NMR (400 MHz, CD₃OD) δ 7.24, 7.20 (s, 1H total), 4.61-4.40 (m, 2H), 4.12 (s, 1H), 3.36-3.32 (m, 1H), 3.28-2.86 (m, 12H), 2.67-2.58 (m, 1H), 2.25-2.21 (m, 1H), 2.16-2.09 (m, 1H), 1.89-1.41 (m, 12H), 1.40-1.14 (m, 4H); MS (ESI) m/z 650.1 (M+H).

S25-11-39:

¹H NMR (400 MHz, CD₃OD) δ 7.31, 7.24 (s, 1H total), 4.76 (d, J=14.0 Hz, 1H), 4.48-4.29 (m, 1H), 4.16 (s, 1H), 3.44-3.34 (m, 1H), 3.25-2.97 (m, 9H), 2.84 (d, J=9.2 Hz, 3H), 2.66-2.59 (m, 1H), 2.30-2.12 (m, 2H), 1.69-1.63 (m, 1H), 1.43, 1.35 (d, J=6.8 Hz, 3H total), 1.16-1.01 (m, 6H); MS (ESI) m/z 596.6 (M+H).

S25-11-40:

¹H NMR (400 MHz, CD₃OD) δ 7.16 (s, 1H), 4.65 (t, J=15.2 Hz, 1H), 4.26 (t, J=16.0 Hz, 1H), 4.07 (s, 1H), 3.35-3.30 (m, 1H), 3.18-2.82 (m, 9H), 2.74 (s, 3H), 2.69-2.52 (m, 1H), 2.18-2.02 (m, 2H), 1.60-1.51 (m, 1H), 1.32 (d, J=6.4 Hz, 3H), 1.01, 0.97 (d, J=6.4 Hz, 6H total); MS (ESI) m/z 596.3 (M+H).

S25-11-41:

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.70 (d, J=13.2 Hz, 1H), 4.36-4.24 (m, 1H), 4.15 (s, 1H), 3.54-3.42 (m, 1H), 3.23-2.96 (m, 9H), 2.79 (s, 3H), 2.69-2.59 (m, 1H), 2.28-2.22 (m, 1H), 1.97-1.92 (m, 1H), 1.71-1.60 (m, 2H), 1.45-1.43 (m, 3H), 1.10-1.03 (m, 3H); MS (ESI) m/z 582.0 (M+H).

S25-11-42:

¹H NMR (400 MHz, CD₃OD) δ 7.27, 7.25, 7.24 (s, 1H total), 4.75-4.63 (m, 1H), 4.51-4.28 (m, 1H), 4.14 (s, 1H), 3.61-3.42 (m, 2H), 3.28-2.96 (m, 10H), 2.67-2.58 (m, 1H), 2.27-2.23 (m, 1H), 1.97-1.88 (m, 1H), 1.81-1.60 (m, 2H), 1.50-1.35 (m, 3H), 1.33-1.26 (m, 3H), 1.08-1.00 (m, 3H); MS (ESI) m/z 596.0 (M+H).

S25-11-43:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (s, 1H), 4.39-4.32 (m, 1H), 4.24-4.20 (m, 1H), 4.04 (s, 1H), 3.11-2.78 (m, 11H), 2.58-2.48 (m, 1H), 2.16-2.08 (m, 1H), 1.68-1.50 (m, 8H), 1.35-1.08 (m, 4H), 0.98-0.81 (m, 2H); MS (ESI) m/z 622.3 (M+H).

S25-11-44:

¹H NMR (400 MHz, CD₃OD) δ 7.48-7.21 (m, 5H), 7.13 (s, 1H), 4.53-4.45 (m, 1H), 4.37-4.30 (m, 1H), 4.12 (s, 1H), 3.46-3.36 (m, 2H), 3.19-2.89 (m, 11H), 2.71-2.56 (m, 1H), 2.24-2.17 (m, 1H), 1.73-1.57 (m, 1H); MS (ESI) m/z 616.2 (M+H).

S25-11-45:

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.42 (d, J=13.6 Hz, 2H), 4.13 (s, 1H), 3.92-3.81 (m, 2H), 3.68-3.59 (m, 2H), 3.58-3.46 (m, 1H), 3.38 (s, 3H), 3.20-2.96 (m, 9H), 2.65-2.54 (m, 1H), 2.38-2.12 (m, 3H), 2.09-1.93 (m, 2H), 1.69-1.59 (m, 1H); MS (ESI) m/z 610.2 (M+H).

S25-11-46:

¹H NMR (400 MHz, CD₃OD) δ 7.14 (s, 1H), 4.90 (d, J=13.2 Hz, 1H), 4.20 (d, J=13.2 Hz, 1H), 4.04 (s, 1H), 3.88-3.78 (m, 1H), 3.72-3.63 (m, 1H), 3.61-3.56 (m, 1H), 3.43-3.28 (m, 5H), 3.19-2.82 (m, 9H), 2.64-2.50 (m, 1H), 2.24-2.06 (m, 3H), 1.93-1.84 (m, 2H), 1.56-1.50 (m, 1H); MS (ESI) m/z 610.0 (M+H).

S25-11-47:

¹H NMR (400 MHz, CD₃OD) δ 7.28 (s, 1H), 4.58-4.44 (m, 2H), 4.12 (s, 1H), 3.92-3.62 (m, 6H), 3.24-2.95 (m, 11H), 2.68-2.58 (m, 1H), 2.29-1.98 (m, 5H), 1.68-1.59 (m, 1H); MS (ESI) m/z 609.0 (M+H).

S25-11-48:

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.30 (d, J=14.0 Hz, 1H), 4.11 (s, 1H), 3.69-3.62 (m, 1H), 3.53-3.41 (m, 1H), 3.40-3.32 (m, 2H), 3.22-2.88 (m, 9H), 2.63-2.52 (m, 1H), 2.42-2.32 (m, 1H), 2.29-2.00 (m, 3H), 1.82-1.77 (m, 1H), 1.71-1.58 (m, 1H), 1.43 (d, J=6.4 Hz, 3H); MS (ESI) m/z 579.9 (M+H).

S25-11-49:

¹H NMR (400 MHz, CD₃OD) δ 7.40 (s, 1H), 4.24 (d, J=13.6 Hz, 1H), 4.09 (s, 1H), 4.01 (d, J=14.0 Hz, 1H), 3.59-3.43 (m, 1H), 3.22-2.94 (m, 11H), 2.61-2.54 (m, 1H), 2.39-2.35 (m, 1H), 2.20-2.13 (m, 2H), 2.00-1.84 (m, 2H), 1.67-1.57 (m, 1H); MS (ESI) m/z 634.1 (M+H).

S25-11-50:

¹H NMR (400 MHz, CD₃OD) δ 7.16 (s, 1H), 4.32-4.29 (m, 2H), 4.05 (s, 1H), 3.11-2.86 (m, 11H), 2.60-2.52 (m, 1H), 2.17-2.14 (m, 1H), 1.59-1.23 (m, 11H), 1.00 (s, 3H); MS (ESI) m/z 622.3 (M+H).

S25-11-51:

¹H NMR (400 MHz, CD₃OD) δ 7.18 (s, 1H), 4.49 (d, J=14.0 Hz, 1H), 4.31 (d, J=13.6 Hz, 1H), 4.04 (s, 1H), 3.54-3.47 (m, 2H), 3.22 (s, 3H), 3.12-2.86 (m, 13H), 2.60-2.48 (m, 1H), 2.16-2.13 (m, 1H), 1.89-1.86 (m, 3H), 1.56-1.46 (m, 3H); MS (ESI) m/z 624.1 (M+H).

S25-11-52:

¹H NMR (400 MHz, CD₃OD) δ 7.30 (s, 1H), 4.60 (d, J=13.6 Hz, 1H), 4.42 (d, J=14.0 Hz, 1H), 4.14 (s, 1H), 3.47-3.35 (m, 2H), 3.26-2.97 (m, 9H), 2.78-2.60 (m, 3H), 2.27-2.23 (m, 1H), 2.02-1.94 (m, 2H), 1.89-1.82 (m, 1H), 1.66 (ddd, J=13.6, 13.6, 13.6 Hz, 1H), 1.02-1.88 (m, 7H); MS (ESI) m/z 608.1 (M+H).

S25-11-53:

¹H NMR (400 MHz, CD₃OD) δ 7.12 (s, 1H), 4.71 (d, J=13.2 Hz, 1H), 4.07 (d, J=13.2 Hz, 1H), 4.00 (s, 1H), 3.57-3.51 (m, 1H), 3.37-3.22 (m, 2H), 3.08-2.78 (m, 9H), 2.54-2.45 (m, 1H), 2.29-2.24 (m, 1H), 2.12-1.89 (m, 3H), 1.69-1.61 (m, 1H), 1.54-1.41 (m, 1H), 1.38 (d, J=6.0 Hz, 3H); MS (ESI) m/z 580.1 (M+H).

S25-11-54:

¹H NMR (400 MHz, CD₃OD) δ 7.05, 6.95, 6.87 (s, 1H total), 4.58-4.41 (m, 1H), 4.41-4.32 (m, 1H), 4.21-4.14 (m, 2H), 3.99 (s, 1H), 3.93-3.72 (m, 2H), 3.61-3.49 (m, 1H), 2.91-2.81 (m, 9H), 2.55-2.41 (m, 1H), 2.12-2.08 (m, 1H), 1.54-1.44 (m, 1H), 1.22-1.04 (m, 3H); MS (ESI) m/z, 565.9 (M+H).

S25-11-55:

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.38 (s, 2H), 4.14 (s, 1H), 3.37-3.34 (m, 1H), 3.25-2.96 (m, 9H), 2.66-2.58 (m, 1H), 2.27-2.16 (m, 2H), 1.69-1.59 (m, 1H), 1.33 (d, J=6.8 Hz, 3H), 1.07 (d, J=6.8 Hz, 3H), 1.00 (d, J=6.8 Hz, 3H); MS (ESI) m/z 582.1 (M+H).

S25-11-56:

¹H NMR (400 MHz, CD₃OD) δ 7.28 (s, 1H), 4.59 (d, J=14.4 Hz, 1H), 4.48 (d, J=14.0H, 1H), 4.12 (s, 1H), 3.58-3.53 (m, 1H), 3.46-3.41 (m, 1H), 3.35-3.33 (m, 1H), 3.28-2.94 (m, 10H), 2.60-2.54 (m, 1H), 2.25-2.20 (m, 1H), 2.01-1.83 (m, 4H), 1.74 (s, 4H), 1.68-1.53 (m, 1H); MS (ESI) m/z 594.0 (M+H).

S25-11-57:

¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.98 (t, J=8.8 Hz, 1H), 4.75 (t, J=8.8 Hz, 1H), 4.52-4.51 (m, 1H), 4.41-4.37 (m, 1H), 4.13 (s, 1H), 3.55 (dt, J=31.6, 9.2 Hz, 2H), 3.21-3.08 (m, 9H), 2.64-2.55 (m, 1H), 2.25-2.21 (m, 1H), 1.67-1.57 (m, 1H); MS (ESI) m/z 558.1 (M+H).

S25-11-58:

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.42-4.38 (m, 1H), 4.27-4.21 (m, 1H), 4.13 (s, 1H), 3.23-2.92 (m, 9H), 2.62-2.58 (m, 1H), 2.27-2.22 (m, 1H), 1.69-1.62 (m, 1H), 1.47 (s, 9H); MS (ESI) m/z 568.0 (M+H).

S25-11-59:

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.48-4.47 (m, 1H), 4.44-4.37 (m, 1H), 4.13 (s, 1H), 4.13-4.07 (m, 3H), 3.90-3.85 (m, 1H), 3.76-3.73 (m, 1H), 3.25-2.96 (m, 9H), 2.70-2.60 (m, 1H), 2.49-2.44 (m, 1H), 2.27-2.22 (m, 1H), 2.14-2.11 (m, 1H), 1.69-1.61 (m, 1H); MS (ESI) m/z 582.1 (M+H).

S25-11-60:

¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.27 (d, J=13.6 Hz, 1H), 4.16 (d, J=14.8 Hz, 1H), 4.12 (s, 1H), 3.24-2.94 (m, 9H), 2.67-2.56 (m, 1H), 2.46-2.44 (m, 2H), 2.24-2.22 (m, 1H), 2.10-1.97 (m, 4H), 1.67-1.62 (m, 4H); MS (ESI) m/z 579.9 (M+H).

S25-11-61:

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.95-4.94 (m, 1H), 4.82-4.76 (m, 1H), 4.11 (s, 1H), 3.69 (dt, J=30.8, 8.8 Hz, 2H), 3.20-2.94 (m, 14H), 2.67-2.57 (m, 1H), 2.24-2.21 (m, 1H), 1.67-1.58 (m, 1H); MS (ESI) m/z 572.0 (M+H).

S25-11-62:

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.70 (d, J=13.6 Hz, 1H), 4.55 (d, J=13.6 Hz, 1H), 4.11 (s, 1H), 3.68-3.63 (m, 2H), 3.48-3.41 (m, 2H), 3.20-2.94 (m, 11H), 2.66-2.58 (m, 1H), 2.24-2.21 (m, 1H), 1.69-1.59 (m, 1H), 1.40 (t, J=7.2 Hz, 3H); MS (ESI) m/z 586.0 (M+H).

S25-11-63:

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.72-4.65 (m, 1H), 4.44-4.27 (m, 1H), 4.13 (s, 1H), 3.03-2.94 (m, 11H), 2.85 (d, J=13.6 Hz, 3H), 2.60-2.57 (m, 1H), 2.25-2.22 (m, 1H), 1.84-1.81 (m, 2H), 1.67-1.57 (m, 1H), 1.02-1.00 (t, J=7.2 Hz, 3H); MS (ESI) m/z 568.0 (M+H).

S25-11-64:

¹H NMR (400 MHz, CD₃OD) δ 7.34 (s, 1H), 4.72 (d, J=13.6 Hz, 1H), 4.59 (d, J=14.0 Hz, 1H), 4.15 (s, 1H), 3.85-3.79 (m, 2H), 3.04-2.95 (m, 9H), 2.63-2.61 (m, 1H), 2.35-2.23 (m, 3H), 1.84-1.81 (m, 2H), 1.66-1.64 (m, 1H), 1.36 (d, J=6.4 Hz, 3H), 1.22 (d, J=6.8 Hz, 3H); MS (ESI) m/z 594.1 (M+H).

S25-11-65:

¹H NMR (400 MHz, CD₃OD) δ 7.28 (s, 1H), 4.73-4.70 (m, 1H), 4.58-4.48 (m, 1H), 4.14 (s, 1H), 3.79-3.66 (m, 1H), 3.53-3.50 (m, 1H), 3.21-2.96 (m, 11H), 2.69-2.58 (m, 1H), 2.28-2.23 (m, 1H), 2.05-1.95 (m, 2H), 1.66-1.62 (m, 1H), 1.28, 1.22 (s, 6H total); MS (ESI) m/z 594.0 (M+H).

S25-11-66:

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.75-4.68 (m, 1H), 4.44-4.41 (m, 1H), 4.30-4.27 (m, 1H), 4.13 (s, 1H), 3.08-2.95 (m, 11H), 2.87 (d, J=14.0 Hz, 3H), 2.68-2.58 (m, 1H), 2.26-2.23 (m, 1H), 1.69-1.66 (m, 4H), 1.00 (d, J=5.2 Hz, 6H); MS (ESI) m/z 596.0 (M+H).

S25-11-67:

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.64-4.60 (m, 1H), 4.52-4.48 (m, 1H), 4.13 (s, 1H), 3.41-3.35 (m, 2H), 3.19-2.96 (m, 11H), 2.64-2.52 (m, 1H), 2.27-2.23 (m, 1H), 1.66-1.63 (m, 4H), 1.41-1.33 (m, 3H), 0.97 (d, J=5.2 Hz, 6H); MS (ESI) m/z 610.0 (M+H).

S25-11-68:

¹H NMR (400 MHz, CD₃OD) δ 7.35 (s, 1H), 4.58-4.55 (m, 1H), 4.45-4.42 (m, 1H), 4.18 (s, 1H), 3.59-3.49 (m, 2H), 3.28-2.97 (m, 11H), 2.63-2.55 (m, 1H), 2.29-2.26 (m, 1H), 1.94-1.82 (m, 5H), 1.66-1.58 (m, 2H); MS (ESI) m/z 580.0 (M+H).

S25-11-69:

¹H NMR (400 MHz, CD₃OD) δ 7.27 (s, 1H), 4.82-4.76 (m, 1H), 4.14 (s, 1H), 4.05-4.02 (m, 1H), 3.60-3.47 (m, 2H), 3.11-2.95 (m, 9H), 2.60-2.55 (m, 1H), 2.22-1.95 (m, 5H), 1.71-1.62 (m, 1H), 1.63 (s, 3H), 1.42 (s, 3H); MS (ESI) m/z 594.0 (M+H).

S25-11-70:

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.80-4.77 (m, 1H), 4.44-4.25 (m, 1H), 4.14 (s, 1H), 3.19-2.96 (m, 11H), 2.90 (d, J=7.6 Hz, 3H), 2.64-2.60 (m, 1H), 2.27-2.22 (m, 2H), 1.69-1.60 (m, 1H), 1.09-1.01 (m, 6H); MS (ESI) m/z 582.0 (M+H).

S25-11-71:

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.75-4.67 (m, 2H), 4.45-4.42 (m, 1H), 4.31-4.27 (m, 1H), 4.14 (s, 1H), 3.18-2.96 (m, 9H), 2.87 (d, J=15.2 Hz, 3H), 2.63-2.60 (m, 1H), 2.27-2.23 (m, 1H), 1.78-1.62 (m, 8H), 1.31-1.25 (m, 4H), 1.04-1.02 (m, 2H); MS (ESI) m/z 636.1 (M+H).

S25-11-72:

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.24-4.20 (m, 1H), 4.13 (s, 1H), 3.53-3.43 (m, 4H), 3.09-2.95 (m, 9H), 2.69-2.62 (m, 1H), 2.48-2.41 (m, 1H), 2.31-2.02 (m, 4H), 1.88-1.79 (m, 1H), 1.69-1.52 (m, 2H), 1.06 (t, J=7.2 Hz, 3H); MS (ESI) m/z 594.1 (M+H).

S25-11-73:

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.75-4.72 (m, 1H), 4.36-4.33 (m, 1H), 4.12 (s, 1H), 3.52-3.48 (m, 3H), 3.09-2.95 (m, 9H), 2.63-2.59 (m, 1H), 2.48-2.41 (m, 1H), 2.25-2.16 (m, 2H), 2.05-1.95 (m, 2H), 1.88-1.79 (m, 1H), 1.66-1.60 (m, 2H), 1.02 (t, J=7.2 Hz, 3H); MS (ESI) m/z 594.0 (M+H).

S25-11-74:

¹H NMR (400 MHz, CD₃OD) δ 7.33-7.25 (m, 6H), 4.74-4.71 (m, 1H), 4.52-4.35 (m, 1H), 4.12 (s, 1H), 3.52-3.48 (m, 2H), 3.14-2.92 (m, 14H), 2.67-2.61 (m, 1H), 2.25-2.21 (m, 1H), 1.67-1.58 (m, 1H); MS (ESI) m/z 630.1 (M+H).

S25-11-75:

¹H NMR (400 MHz, CD₃OD) δ 7.39 (s, 1H), 4.56-4.45 (m, 2H), 4.12 (s, 1H), 3.74-3.60 (m, 6H), 3.04-2.95 (m, 11H), 2.81 (s, 3H), 2.61-2.57 (m, 1H), 2.23-2.20 (m, 1H), 2.12-2.02 (m, 4H), 1.68-1.59 (m, 1H); MS (ESI) m/z 623.1 (M+H).

S25-11-76:

¹H NMR (400 MHz, CD₃OD) δ 7.41 (s, 1H), 4.59-4.48 (m, 2H), 4.12 (s, 1H), 3.74-3.58 (m, 6H), 3.20-2.95 (m, 13H), 2.61-2.57 (m, 1H), 2.24-2.11 (m, 5H), 1.68-1.58 (m, 1H), 1.35 (t, J=7.2 Hz, 3H); MS (ESI) m/z 637.1 (M+H).

S25-11-77:

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.80-4.69 (m, 1H), 4.43-4.26 (m, 1H), 4.13 (s, 1H), 3.41-3.35 (m, 2H), 3.03-2.94 (m, 9H), 2.85 (d, J=14.8 Hz, 3H), 2.61-2.57 (m, 1H), 2.25-2.21 (m, 1H), 1.74-1.61 (m, 3H), 0.99, 0.97 (s, 9H total); MS (ESI) m/z 610.1 (M+H).

S25-11-78:

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.78-4.74 (m, 1H), 4.44-4.26 (m, 1H), 4.14 (s, 1H), 3.30-2.96 (m, 11H), 2.90 (d, J=9.6 Hz, 3H) 2.63-2.59 (m, 1H), 2.40-2.37 (m, 1H), 2.27-2.23 (m, 1H), 1.97-1.91 (m, 2H), 1.73-1.63 (m, 5H), 1.33-1.27 (m, 2H); MS (ESI) m/z 608.2 (M+H).

S25-11-79:

¹H NMR (400 MHz, CD₃OD) δ 7.24, 7.21 (s, 1H total), 4.69-4.42 (m, 2H), 4.13 (s, 1H), 3.09-2.96 (m, 13H), 2.65-2.58 (m, 1H), 2.31-2.22 (m, 2H), 1.68-1.52 (m, 7H), 1.40 (t, J=7.2 Hz, 3H), 1.28-1.21 (m, 2H); MS (ESI) m/z 622.4 (M+H).

S25-11-80:

¹H NMR (400 MHz, CD₃OD) δ 7.16, 7.14 (s, 1H total), 4.69-4.66 (m, 1H), 4.35-4.17 (m, 1H), 4.05 (s, 1H), 3.20-2.87 (m, 11H), 2.80 (d, J=11.2 Hz, 3 H), 2.56-2.49 (m, 1H), 2.17-2.14 (m, 1H), 1.78-1.56 (m, 7H), 1.27-1.00 (m, 3H), 0.97-0.92 (m, 2H); MS (ESI) m/z 622.0 (M+H.

S25-11-81:

¹H NMR (400 MHz, CD₃OD) δ 7.23, 7.19 (s, 1H total), 4.65-4.40 (m, 2H), 4.11 (s, 1H), 3.10-2.86 (m, 13H), 2.67-2.64 (m, 1H), 2.24-2.21 (m, 1H), 1.82-1.64 (m, 7H), 1.39-1.18 (m, 6H), 1.01-0.98 (m, 2H); MS (ESI) m/z 636.1 (M+H).

S25-11-82:

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.58 (d, J=14.4 Hz, 1H), 4.45 (d, J=14.4 Hz, 1H), 4.13 (s, 1H), 3.47-3.41 (m, 2H), 3.12-2.95 (m, 11H), 2.69-2.58 (m, 1H), 2.26-2.22 (m, 1H), 1.78-1.62 (m, 5H), 1.12 (s, 3H), 1.04 (s, 3H), MS (ESI) m/z 608.3 (M+H).

S25-11-83:

¹H NMR (400 MHz, CD₃OD) δ 7.35, 7.26 (s, 1H total), 4.70-4.67 (m, 1H), 4.54-4.34 (m, 1H), 4.15 (s, 1H), 3.42-3.38 (m, 1H), 3.21-2.86 (m, 13H), 2.69-2.63 (m, 1H), 2.24-2.17 (m, 2H), 2.02-1.69 (m, 8H), 0.93, 0.84 (t, J=6.8 Hz, 3H total); MS (ESI) m/z 622.4 (M+H).

S25-11-84:

¹H NMR (400 MHz, CD₃OD) δ 7.37, 7.36, 7.31, 7.30 (s, 1H total), 4.65-4.50 (m, 2H), 4.15 (s, 1H), 3.48-3.38 (m, 2H), 3.25-2.96 (m, 11H), 2.68-2.53 (m, 1H), 2.27-2.24 (m, 1H), 2.05-1.63 (m, 8H), 1.47-1.44 (m, 3H), 0.91, 0.79 (t, J=7.2 Hz, 3H total); MS (ESI) m/z 636.6 (M+H).

S25-11-85:

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.71-4.68 (m, 1H), 4.31-4.22 (m, 1H), 4.12 (s, 1H), 3.81-3.73 (m, 1H), 3.18-2.95 (m, 9H), 2.77 (s, 3H), 2.67-2.59 (m, 1H), 2.25-2.21 (m, 1H), 1.68-1.59 (m, 1H), 1.42 (d, J=5.6 Hz, 6H); MS (ESI) m/z 568.0 (M+H).

S25-11-86:

¹H NMR (400 MHz, CD₃OD) δ 7.26, 7.24 (s, 1H total), 4.74-4.62 (m, 1H), 4.48-4.28 (m, 1H), 4.14 (s, 1H), 3.88-3.72 (m, 1H), 3.28-2.91 (m, 11H), 2.62-2.59 (m, 1H), 2.26-2.23 (m, 1H), 1.66-1.63 (m, 1H), 1.51-1.39 (m, 6H), 1.33, 1.27 (t, J=7.2 Hz, 3H total); MS (ESI) m/z 582.1 (M+H).

S25-11-87:

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.67-4.59 (m, 1H), 4.31-4.28 (m, 0.5H), 4.19-4.15 (m, 0.5H), 4.14 (s, 1H), 3.95-3.91 (m, 1H), 3.18-2.96 (m, 9H), 2.73 (d, J=7.2 Hz, 3H), 2.69-2.59 (m, 1H), 2.41-2.38 (m, 3H), 2.27-2.24 (m, 2H), 1.91-1.81 (m, 2H), 1.69-1.59 (m, 1H); MS (ESI) m/z 580.0 (M+H).

S25-11-88:

¹H NMR (400 MHz, CD₃OD) δ 7.29 (s, 1H), 4.32-4.21 (m, 3H), 4.18-4.16 (m, 1H), 4.15 (s, 1H), 3.83-3.72 (m, 2H), 3.11-2.96 (m, 10H), 2.82 (s, 3H), 2.65-2.61 (m, 1H), 2.46-2.35 (m, 2H), 2.26-2.23 (m, 1H), 1.69-1.63 (m, 1H); MS (ESI) m/z 596.0 (M+H).

S25-11-89:

¹H NMR (400 MHz, CD₃OD) δ 7.30-7.22 (m, 5H), 4.76-4.45 (m, 4H), 4.14 (s, 1H), 3.51-3.45 (m, 4H), 3.19-2.96 (m, 10H), 2.65-2.61 (m, 1H), 2.26-2.23 (m, 1H), 1.67-1.60 (m, 1H), 1.40-1.35 (m, 3H); MS (ESI) m/z 656.0 (M+H).

S25-11-90:

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.72-4.61 (m, 1H), 4.51-4.42 (m, 1H), 4.33-4.10 (m, 4H), 3.90-3.60 (m, 3H), 3.12-2.89 (m, 10H), 2.69-2.58 (m, 1H), 2.41-2.20 (m, 3H), 1.65-1.62 (m, 1H), 1.37-1.27 (m, 3H); MS (ESI) m/z 610.2 (M+H).

S25-11-91:

¹H NMR (400 MHz, CD₃OD) δ 7.24, 7.22 (s, 1H total), 4.78-4.74 (m, 1H), 4.31-4.20 (m, 1H), 4.13 (s, 1H), 3.47-3.37 (m, 1H), 3.21-2.92 (m, 9H), 2.79 (d, J=5.2 Hz, 3H), 2.66-2.59 (m, 1H), 2.25-2.22 (m, 1H), 2.12-2.09 (m, 2H), 2.01-1.92 (m, 2H), 1.74-1.60 (m, 4H), 1.46-1.40 (m, 2H), 1.32-1.22 (m, 1H); MS (ESI) m/z 608.3 (M+H).

S25-11-92:

¹H NMR (400 MHz, CD₃OD) δ 7.25, 7.24 (s, 1H total), 4.83-4.82 (m, 1H), 4.22-4.10 (m, 1H), 4.12 (s, 1H), 3.20-2.90 (m, 9H), 2.70 (d, J=10.8 Hz, 3H), 2.65-2.57 (m, 1H), 2.24-2.21 (m, 1H), 1.67-1.61 (m, 1H), 1.53 (s, 9H); MS (ESI) m/z 582.1 (M+H).

S25-11-93:

¹H NMR (400 MHz, CD₃OD) δ 7.35, 7.30 (s, 1H total), 4.76-4.73 (m, 1H), 4.49-4.38 (m, 1H), 4.11 (s, 1H), 3.53-3.47 (m, 1H), 3.18-2.91 (m, 10H), 2.66-2.59 (m, 1H), 2.23-2.20 (m, 1H), 1.67-1.61 (m, 1H), 1.56, 1.53 (s, 9H total), 1.13, 1.03 (t, J=7.2 Hz, 3H total); MS (ESI) m/z 596.1 (M+H).

S25-11-94:

¹H NMR (400 MHz, CD₃OD) δ 7.23, 7.22 (s, 1H total), 4.82-4.73 (m, 1H), 4.26-4.14 (m, 1H), 4.11 (s, 1H), 3.22-2.92 (m, 9H), 2.71, 2.68 (s, 3H total), 2.65-2.58 (m, 1H), 2.24-2.21 (m, 1H), 1.95-1.87 (m, 2H), 1.67-1.58 (m, 1H), 1.50, 1.48 (s, 6H total), 1.06 (t, J=7.2 Hz, 3H); MS (ESI) m/z 596.0 (M+H).

S25-11-95:

¹H NMR (400 MHz, CD₃OD) δ 7.37, 7.32 (s, 1H total), 4.79-4.75 (m, 1H), 4.52-4.41 (m, 1H), 4.12 (s, 1H), 3.58-3.52 (m, 1H), 3.20-2.92 (m, 10H), 2.68-2.60 (m, 1H), 2.25-2.21 (m, 1H), 1.96-1.90 (m, 2H), 1.69-1.59 (m, 1H), 1.53-1.50 (m, 6H), 1.07-1.01 (m, 6H); MS (ESI) m/z 610.1 (M+H).

S25-11-96:

¹H NMR (400 MHz, CD₃OD) δ 7.26, 7.24 (s, 1H total), 4.79-4.73 (m, 1H), 4.26-4.15 (m, 1H), 4.13 (s, 1H), 3.21-2.92 (m, 9H), 2.70, 2.68 (s, 3H total), 2.70-2.58 (m, 1H), 2.25-2.21 (m, 1H), 1.96-1.93 (m, 1H), 1.82-1.79 (m, 1H), 1.67-1.63 (m, 7H), 1.13 (s, 9H); MS (ESI) m/z 638.1 (M+H).

S25-11-97:

¹H NMR (400 MHz, CD₃OD) δ 7.40, 7.35 (s, 1H total), 4.78-4.75 (m, 1H), 4.52-4.41 (m, 1H), 4.12 (s, 1H), 3.63-3.55 (m, 1H), 3.07-2.95 (m, 10H), 2.67-2.60 (m, 1H), 2.25-2.22 (m, 1H), 1.94-1.86 (m, 2H), 1.82-1.79 (m, 7H), 1.13, 1.11 (s, 9H total), 0.98 (t, J=7.2 Hz, 3H); MS (ESI) m/z 652.1 (M+H).

S25-11-98:

¹H NMR (400 MHz, CD₃OD) δ 7.28 (s, 1H), 4.80-4.76 (m, 1H), 4.41-4.30 (m, 1H), 4.15 (s, 1H), 3.21-2.95 (m, 10H), 2.84, 2.89 (s, 3H total), 2.67-2.58 (m, 1H), 2.28-2.25 (m, 1H), 1.69-1.60 (m, 1H), 1.54-1.49 (m, 3H), 1.23-1.20 (m, 1H), 0.85-0.71 (m, 3H), 0.52-0.41 (m, 1H); MS (ESI) m/z 594.2 (M+H).

S25-11-99:

¹H NMR (400 MHz, CD₃OD) δ 7.27, 7.26 (s, 1H total), 4.97-4.94 (m, 1H), 4.80-4.44 (m, 1H), 4.62-4.58 (m, 0.4H), 4.32-4.29 (m, 0.6H), 4.14 (s, 1H), 3.21-2.92 (m, 11H), 2.68-2.61 (m, 1H), 2.27-2.23 (m, 1H), 1.70-1.60 (m, 1H), 1.51-1.47 (m, 3H), 1.37, 1.24 (t, J=7.2 Hz, 3H total), 1.35-1.31 (m, 1H), 0.85-0.82 (m, 2H), 0.64-0.39 (m, 2H); MS (ESI) m/z 608.2 (M+H).

S25-11-100:

¹H NMR (400 MHz, CD₃OD) δ 7.30 (s, 1H), 4.77-4.73 (m, 2H), 4.13 (s, 1H), 3.24-2.92 (m, 12H), 2.65-2.57 (m, 1H), 2.25-2.22 (m, 1H), 1.64-1.61 (m, 2H), 1.19-1.14 (m, 2H), 0.92-0.81 (m, 2H), 0.77-0.75 (m, 2H), 0.40-0.32 (m, 2H); MS (ESI) m/z 606.0 (M+H).

S25-11-101:

¹H NMR (400 MHz, CD₃OD) δ 7.30 (s, 1H), 4.82-4.75 (m, 2H), 4.12 (s, 1H), 3.70-3.62 (m, 1H), 3.21-2.92 (m, 10H), 2.65-2.57 (m, 1H), 2.24-2.21 (m, 1H), 1.64-1.62 (m, 2H), 1.41-1.37 (m, 4H), 1.00-0.68 (m, 5H), 0.51-0.24 (m, 2H); MS (ESI) m/z 620.1 (M+H).

S25-11-102:

¹H NMR (400 MHz, CD₃OD) δ 7.26, 7.21 (s, 1H total), 4.75-4.72 (m, 1H), 4.34-4.28 (m, 1H), 4.13 (s, 1H), 3.71-3.62 (m, 1H), 3.11-2.92 (m, 9H), 2.85-2.78 (m, 3H), 2.65-2.58 (m, 1H), 2.39-2.35 (m, 1H), 2.25-2.22 (m, 1H), 2.18-2.12 (m, 1H), 1.73-1.53 (m, 8H), 1.43-1.28 (m, 1H); MS (ESI) m/z 620.0 (M+H).

S25-11-103:

¹H NMR (400 MHz, CD₃OD) δ 7.30, 7.28, 7.25 (s, 1H total), 4.79-4.75 (m, 1H), 4.66-4.48 (m, 1H), 4.13 (s, 1H), 3.82-3.78 (m, 1H), 3.22-2.92 (m, 11H), 2.69-2.60 (m, 2H), 2.40-2.38 (m, 1H), 2.26-2.23 (m, 1H), 2.13-2.07 (m, 1H), 1.68-1.41 (m, 8H), 1.29, 1.23 (t, J=7.2 Hz, 3H total); MS (ESI) m/z 634.1 (M+H).

S25-11-104:

¹H NMR (400 MHz, CD₃OD) δ 7.37, 7.30 (s, 1H total), 4.79-4.76 (m, 1H), 4.60-4.42 (m, 1H), 4.15 (s, 1H), 3.29-2.98 (m, 14H), 2.64-2.60 (m, 1H), 2.28-2.25 (m, 1H), 1.72-1.65 (m, 1H), 1.15, 1.07 (s, 9H total); MS (ESI) m/z 596.2 (M+H).

S25-11-105:

¹H NMR (400 MHz, CD₃OD) δ 7.38, 7.31 (s, 1H total), 4.69-4.62 (m, 2H), 4.14 (s, 1H), 3.52-3.41 (m, 2H), 3.28-2.88 (m, 11H), 2.70-2.61 (m, 1H), 2.28-2.24 (m, 1H), 1.71-1.61 (m, 1H), 1.51-1.44 (m, 3H), 1.09, 0.99 (s, 9H total); MS (ESI) m/z 610.0 (M+H).

S25-11-106:

¹H NMR (400 MHz, CD₃OD) δ 7.42 (s, 1H), 4.76-4.72 (m, 1H), 4.57-4.53 (m, 1H), 4.13 (s, 1H), 3.20-2.92 (m, 13H), 2.61-2.58 (m, 1H), 2.26-2.23 (m, 1H), 1.69-1.60 (m, 1H), 1.39, 1.38 (s, 3H total), 0.97 (s, 9H); MS (ESI) m/z 610.0 (M+H).

S25-11-106:

¹H NMR (400 MHz, CD₃OD) δ 7.29 (s, 1H), 4.97-4.94 (m, 1H), 4.43-4.28 (m, 1H), 4.14 (s, 1H), 3.94-3.63 (m, 1H), 3.21-2.98 (m, 9H), 2.85, 2.74 (s, 3H total), 2.67-2.59 (m, 1H), 2.50-2.42 (m, 1H), 2.27-2.24 (m, 1H), 1.94-1.93 (m, 1H), 1.82-1.52 (m, 6H), 1.25, 1.18 (s, 3H total), 0.98-0.93 (m, 6H); MS (ESI) m/z 662.0

S25-11-107:

¹H NMR (400 MHz, CD₃OD) δ 7.38 (s, 1H), 4.95-4.92 (m, 1H), 4.43-4.40 (m, 1H), 4.14 (s, 1H), 3.79-3.76 (m, 1H), 3.21-2.91 (m, 11H), 2.69-2.62 (m, 1H), 2.47-2.41 (m, 1H), 2.27-2.24 (m, 1H), 1.82-1.58 (m, 6H), 1.47-1.44 (m, 1H), 1.26-1.21 (m, 6H), 1.00-0.98 (m, 5H), 0.93 (s, 1H); MS (ESI) m/z 676.1 (M+H).

S25-11-108:

¹H NMR (400 MHz, CD₃OD) δ 7.28, 7.25 (s, 1H total), 4.76-4.72 (m, 1H), 4.50-4.32 (m, 1H), 4.14 (s, 1H), 3.28-2.95 (m, 11H), 2.91 (s, 3H), 2.66-2.60 (m, 2H), 2.46-2.44 (m, 1H), 2.27-2.15 (m, 2H), 2.02-1.94 (m, 5H), 1.66-1.60 (m, 1H), 1.22 (s, 3H), 1.08-1.06 (m, 1H), 0.94, 0.91 (s, 3H total); MS (ESI) m/z 662.1 (M+H).

S25-11-109

¹H NMR (400 MHz, CD₃OD) δ 7.28, 7.26, 7.25 (s, 1H total), 4.67-4.61 (m, 1H), 4.57-4.50 (m, 1H), 4.42-4.36 (m, 1H), 4.14 (s, 1H), 3.38-3.37 (m, 1H), 3.28-2.88 (m, 11H), 2.67-2.45 (m, 3H), 2.28-2.05 (m, 2H), 2.00-1.91 (m, 5H), 1.67-1.61 (m, 1H), 1.41 (t, J=5.6 Hz, 3H), 1.26-1.17 (m, 3H), 1.06-1.04 (m, 2H), 0.88-0.83 (m, 2H); MS (ESI) m/z 676.1 (M+H).

S25-11-110:

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.72-4.58 (m, 2H), 4.12 (s, 1H), 3.05-2.96 (m, 13H), 2.70-2.59 (m, 1H), 2.25-2.22 (m, 1H), 1.70-1.61 (m, 1H), 1.01-0.93 (m, 4H); MS (ESI) m/z 566.0 (M+H).

S25-11-111:

¹H NMR (400 MHz, CD₃OD) δ 7.27 (s, 1H), 4.72-4.64 (m, 2H), 4.14 (s, 1H), 3.50-3.44 (m, 1H), 3.21-2.82 (m, 11H), 2.62-2.59 (m, 1H), 2.26-2.23 (m, 1H), 1.70-1.60 (m, 1H), 1.51-1.43 (m, 3H), 1.10-0.80 (m, 3H), 0.70-0.32 (m, 1H); MS (ESI) m/z 580.0 (M+H).

S25-11-112:

¹H NMR (400 MHz, CD₃OD) δ 7.28 (s, 1H), 4.76-4.67 (m, 2H), 4.12 (s, 1H), 3.45-3.39 (m, 1H), 3.18-2.91 (m, 11H), 2.67-2.57 (m, 1H), 2.25-2.22 (m, 1H), 1.68-1.58 (m, 1H), 1.31-1.25 (m, 2H), 1.08-0.92 (m, 1H), 0.84-0.82 (m, 3H), 0.52-0.47 (m, 3H); MS (ESI) m/z 606.0 (M+H).

S25-11-113:

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.63-4.59 (m, 1H), 4.49-4.46 (m, 1H), 4.12 (s, 1H), 3.24-2.92 (m, 13H), 2.68-2.58 (m, 1H), 2.25-2.22 (m, 1H), 1.74-1.63 (m, 8H), 1.43-1.38 (m, 1H), 1.36-1.24 (m, 6H), 1.02-0.97 (m, 2H); MS (ESI) m/z 650.2 (M+H).

S25-11-114:

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.82-4.58 (m, 2H), 4.13 (s, 1H), 3.28-2.92 (m, 13H), 2.68-2.59 (m, 1H), 2.26-2.23 (m, 1H), 1.73-1.63 (m, 8H), 1.28-1.17 (m, 5H), 1.02-0.97 (m, 2H), 0.81-0.77 (m, 2H), 0.47-0.41 (m, 2H); MS (ESI) m/z 676.2 (M+H).

S25-11-115:

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.97 (m, 1H), 4.64 (m, 1H), 4.68-4.62 (m, 1H), 4.12 (s, 1H), 3.85-3.58 (m, 1H), 3.25-2.90 (m, 13H), 2.64-2.57 (m, 1H), 2.24-2.21 (m, 1H), 1.68-1.58 (m, 1H), 1.22-1.15 (m, 1H), 0.81-0.79 (m, 2H), 0.48-0.44 (m, 2H); MS (ESI) m/z 612.1 (M+H).

S25-11-116:

¹H NMR (400 MHz, CD₃OD) δ 7.28, 7.24 (s, 1H total), 4.78-4.74 (m, 1H), 4.65-4.62 (m, 1H), 4.08 (s, 1H), 3.78-3.72 (m, 2H), 3.66-3.63 (m, 1H), 3.52-3.43 (m, 1H), 3.20-2.82 (m, 12H), 2.64-2.55 (m, 1H), 2.20-2.17 (m, 1H), 1.65-1.56 (m, 1H), 1.17-1.16 (m, 7H), 0.76-0.72 (m, 2H), 1.43-0.39 (m, 2H); MS (ESI) m/z 652.1 (M+H).

S25-11-117:

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.81-4.77 (m, 1H), 4.62-4.58 (m, 1H), 4.10 (s, 1H), 3.36-3.33 (m, 1H), 3.22-2.91 (m, 12H), 2.64-2.55 (m, 1H), 2.21-2.18 (m, 1H), 1.65-1.55 (m, 1H), 1.17-1.11 (m, 2H), 0.76-0.73 (m, 4H), 0.43-0.32 (m, 4H); MS (ESI) m/z 620.0 (M+H).

S25-11-118:

¹H NMR (400 MHz, CD₃OD) δ 7.18 (s, 1H), 4.75-4.72 (m, 0.5H), 4.55 (m, 1H), 4.46-4.42 (m, 0.5H), 4.10 (s, 1H), 3.42-3.40 (m, 1H), 3.12-2.85 (m, 13H), 2.65-2.55 (m, 1H), 2.21-2.10 (m, 4H), 1.99-1.97 (m, 1H), 1.91-1.81 (m, 4H), 1.65-1.56 (m, 1H), 1.15-1.09 (m, 1H), 0.77-0.73 (m, 2H), 0.42-0.36 (m, 2H); MS (ESI) m/z 634.0 (M+H).

S25-11-119:

¹H NMR (400 MHz, CD₃OD) δ 7.42, 7.38 (s, 1H total), 4.82-4.71 (m, 2H), 4.13 (s, 1H), 3.48-3.38 (m, 2H), 3.28-2.83 (m, 11H), 2.68-2.60 (m, 1H), 2.26-2.23 (m, 1H), 1.69-1.60 (m, 1H), 1.34-1.28 (m, 1H), 1.05, 0.95 (s, 9H total), 0.88-0.84 (m, 2H), 0.53-0.48 (m, 2H); MS (ESI) m/z 636.1 (M+H).

S25-11-120:

¹H NMR (400 MHz, CD₃OD) δ 7.43, 7.31 (s, 1H total), 4.89-4.82 (m, 0.5H), 4.71-4.67 (m, 1H), 4.32-4.29 (m, 0.5H), 4.14 (s, 1H), 3.50-3.47 (m, 2H), 3.22-2.96 (m, 10H), 2.67-2.64 (m, 1H), 2.27-2.23 (m, 2H), 1.66-1.60 (m, 1H), 1.44-1.28 (m, 6H), 1.15-0.99 (m, 6H); MS (ESI) m/z 610.0 (M+H).

S25-11-121:

¹H NMR (400 MHz, CD₃OD) δ 7.27 (s, 1H), 4.62-4.38 (m, 2H), 4.14 (s, 1H), 4.01 (s, 1H), 3.25-2.88 (m, 11H), 2.69-2.57 (m, 1H), 2.45-2.33 (m, 2H), 2.27-2.05 (m, 2H), 2.02-1.71 (m, 3H), 1.69-1.58 (m, 1H), 1.48-1.37 (m, 3H); MS (ESI) m/z 594.2 (M+H).

S25-11-122:

¹H NMR (400 MHz, CD₃OD) δ 7.28 (s, 1H), 4.82-4.75 (m, 1H), 4.50-4.31 (m, 1H), 4.15 (s, 1H), 3.99-3.75 (m, 3H), 3.52-3.35 (m, 3H), 3.25-2.82 (m, 13H), 2.69-2.63 (m, 1H), 2.27-2.24 (m, 2H), 1.70-1.60 (m, 2H); MS (ESI) m/z 610.2 (M+H).

S25-11-123:

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.73-4.49 (m, 2H), 4.13 (s, 1H), 3.95-3.75 (m, 3H), 3.48-3.30 (m, 3H), 3.25-2.70 (m, 12H), 2.69-2.60 (m, 1H), 2.26-2.23 (m, 2H), 1.72-1.60 (m, 2H), 1.40 (t, J=7.2 Hz, 3H); MS (ESI) m/z 624.2 (M+H).

S25-11-124:

¹H NMR (400 MHz, CD₃OD) δ 7.31-7.21 (m, 5H), 4.40-4.36 (m, 2H), 4.14 (s, 1H), 3.60-3.33 (m, 5H), 3.25-2.82 (m, 9H), 2.79 (s, 3H), 2.68-2.61 (m, 1H), 2.26-2.23 (m, 1H), 1.69-1.59 (m, 1H); MS (ESI) m/z 642.1 (M+H).

S25-11-125:

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.81-4.71 (m, 1H), 4.44-4.26 (m, 1H), 4.15 (s, 1H), 3.84-3.82 (m, 1H), 3.21-2.87 (m, 9H), 2.78 (s, 3H), 2.69-2.55 (m, 1H), 2.35-2.17 (m, 3H), 1.95-1.73 (m, 4H), 1.69-1.59 (m, 3H); MS (ESI) m/z 594.1 (M+H).

S25-11-126:

¹H NMR (400 MHz, CD₃OD) δ 7.26, 7.24 (s, 1H total), 4.75-4.71 (m, 0.6H), 4.61-4.59 (m, 1H), 4.52-4.48 (m, 0.5H), 4.14 (s, 1H), 3.89-3.82 (m, 1H), 3.25-2.95 (m, 11H), 2.65-2.62 (m, 1H), 2.26-2.14 (m, 3H), 1.95-1.84 (m, 4H), 1.72-1.62 (m, 3H), 1.35, 1.28 (t, J=7.2 Hz, 3H total); MS (ESI) m/z 608.2 (M+H).

S25-11-127:

¹H NMR (400 MHz, CD₃OD) δ 7.21, 7.20 (s, 1H total), 4.80-4.68 (m, 1H), 4.44-4.40 (m, 0.5H), 4.28-4.25 (m, 0.5H), 4.11 (s, 1H), 3.59-3.42 (m, 2H), 3.24-2.85 (m, 10H), 2.67-2.56 (m, 1H), 2.23-1.90 (m, 5H), 1.83-1.58 (m, 4H), 1.45-1.24 (m, 6H); MS (ESI) m/z 622.3 (M+H).

S25-11-128:

¹H NMR (400 MHz, CD₃OD) δ 7.33, 7.24 (s, 1H total), 5.03-4.96 (m, 2H), 4.14-4.10 (m, 3H), 3.87-3.62 (m, 2H), 3.48-3.38 (m, 1H), 3.25-2.89 (m, 9H), 2.70-2.63 (m, 1H), 2.27-2.24 (m, 1H), 1.71-1.55 (m, 3H), 1.35-1.19 (m, 4H), 0.98 (s, 6H); MS (ESI) m/z 640.0 (M+H).

S25-11-129:

¹H NMR (400 MHz, CD₃OD) δ 7.69-7.49 (m, 5H), 7.13 (s, 1H), 4.49-4.32 (m, 4H), 4.13 (s, 1H), 3.23-2.88 (m, 9H), 2.70-2.56 (m, 1H), 2.29-2.22 (m, 1H), 1.68-1.59 (m, 1H); MS (ESI) m/z 602.1 (M+H).

S25-11-130:

¹H NMR (400 MHz, CD₃OD) δ 7.15 (s, 1H), 4.53 (d, J=14.0 Hz, 1H), 4.43 (d, J=12.4 Hz, 1H), 4.12 (s, 1H), 3.23-2.86 (m, 10H), 2.67-2.55 (m, 1H), 2.25-2.21 (m, 1H), 1.67-1.57 (m, 1H), 0.96-0.95 (m, 4H); MS (ESI) m/z 552.1 (M+H).

S25-11-131:

¹H NMR (400 MHz, CD₃OD) δ 7.53-7.49 (m, 5H), 7.31, 7.16 (s, 1H total), 4.63-4.23 (m, 4H), 4.13 (s, 1H), 3.22-2.89 (m, 11H), 2.70-2.56 (m, 1H), 2.26-2.22 (m, 1H), 1.69-1.59 (m, 1H), 1.48 (t, J=7.2 Hz, 3H); MS (ESI) m/z 630.1 (M+H).

S25-11-132:

¹H NMR (400 MHz, CD₃OD) δ 7.67-7.46 (m, 5H), 7.23, 7.18 (s, 1H total), 4.63-4.39 (m, 4H), 4.12 (s, 1H), 3.22-2.81 (m, 11H), 2.68-2.53 (m, 1H), 2.23-2.20 (m, 1H), 1.66-1.58 (m, 1H), 1.36-1.31 (m, 1H), 0.85-0.78 (m, 2H), 0.47-0.42 (m, 2H); MS (ESI) m/z 656.2 (M+H).

S25-11-133:

¹H NMR (400 MHz, CD₃OD) δ 7.55-7.52 (m, 5H), 7.14, 6.99 (s, 1H total), 4.62-4.55 (m, 0.4H), 4.40-4.25 (m, 1H), 4.13-3.98 (m, 1.5H), 3.22-2.88 (m, 10H), 2.71-2.56 (m, 1H), 2.24-2.21 (m, 1H), 1.80-1.1.78 (m, 3H), 1.66-1.59 (m, 1H); MS (ESI) m/z 616.2 (M+H).

S25-11-134:

¹H NMR (400 MHz, CD₃OD) δ 7.21-6.99 (m, 5H), 6.89, 6.79 (s, 1H total), 4.72-4.62 (m, 1H), 3.86 (s, 1H), 3.62-3.52 (m, 1H), 3.32-3.10 (m, 2H), 3.00-2.55 (m, 11H), 2.40-2.39 (m, 1H), 2.02-1.95 (m, 1H), 1.42-1.33 (m, 1H), 1.29-1.09 (m, 6H), 0.93-0.80 (m, 1H), 0.52-0.42 (m, 2H), 0.10-0.01 (m, 2H); MS (ESI) m/z 698.1 (M+H).

S25-11-135:

¹H NMR (400 MHz, CD₃OD) δ 7.48 (s, 1H), 4.83-4.62 (m, 2H), 4.13 (s, 1H), 3.85-3.63 (m, 6H), 3.23-2.87 (m, 13H), 2.68-2.58 (m, 1H), 2.28-2.00 (m, 5H), 1.68-1.59 (m, 1H), 1.29-1.18 (m, 1H), 0.86-0.78 (m, 2H), 0.52-0.42 (m, 2H); MS (ESI) m/z 663.3 (M+H).

S25-11-136:

¹H NMR (400 MHz, CD₃OD) δ 7.32, 7.24, 7.23 (s, 1H total), 4.78-4.50 (m, 2H), 4.12, 3.87 (s, 1H total), 3.25-2.95 (m, 13H), 2.68-2.62 (m, 1H), 2.25-2.11 (m, 1H), 1.78-1.54 (m, 3H), 1.20-1.10 (m, 1H), 1.00, 0.97, 0.94 (s, 9H total), 0.86-0.75 (m, 2H), 0.51-0.41 (m, 2H); MS (ESI) m/z 650.1 (M+H).

S25-11-137:

¹H NMR (400 MHz, CD₃OD) δ 7.52-7.19 (m, 6H), 4.76-4.57 (m, 2H), 4.14 (s, 1H), 3.52-3.36 (m, 4H), 3.22-2.90 (m, 11H), 2.67-2.60 (m, 1H), 2.27-2.23 (m, 1H), 1.71-1.61 (m, 1H), 1.47 (t, J=14.0 Hz, 3H); MS (ESI) m/z 644.5 (M+H).

S25-11-138:

¹H NMR (400 MHz, CD₃OD) δ 7.53-7.28 (m, 6H), 4.76-4.57 (m, 2H), 4.16 (s, 1H), 3.68-3.40 (m, 3H), 3.23-2.91 (m, 12H), 2.71-2.61 (m, 1H), 2.28-2.25 (m, 1H), 1.71-1.65 (m, 1H), 1.32-1.18 (m, 1H), 0.89-0.78 (m, 2H), 0.55-0.45 (m, 2H); MS (ESI) m/z 670.1 (M+H).

S25-11-139:

¹H NMR (400 MHz, CD₃OD) δ 7.29, 7.28 (s, 1H total), 4.83-4.75 (m, 1H), 4.71-4.65 (m, 1H), 4.15 (s, 1H), 3.54-3.36 (m, 1H), 3.23-2.98 (m, 12H), 2.69-2.61 (m, 1H), 2.42-2.25 (m, 2H), 2.02-1.97 (m, 2H), 1.74-1.62 (m, 5H), 1.35-1.16 (m, 3H), 0.87-0.78 (m, 2H), 0.54-0.44 (m, 2H); MS (ESI) m/z 648.1 (M+H).

S25-11-140:

¹H NMR (400 MHz, CD₃OD) δ 7.34 (s, 1H), 5.12-5.03 (m, 2H), 4.16 (s, 1H), 3.75-3.73 (m, 1H), 3.25-2.96 (m, 10H), 2.69-2.62 (m, 1H), 2.31-2.25 (m, 1H), 1.76-1.65 (m, 2H), 1.61-1.41 (m, 1H), 1.28-1.16 (m, 1H), 1.04-0.95 (m, 1H), 0.94-0.73 (m, 6H), 0.55-0.30 (m, 4H); MS (ESI) m/z 646.1 (M+H)

S25-11-141:

¹H NMR (400 MHz, CD₃OD) δ 7.33, 7.32, 7.29 (s, 1H total), 4.85-4.61 (m, 2H), 4.15 (s, 1H), 3.21-2.90 (m, 13H), 2.70-2.60 (m, 1H), 2.29-2.25 (m, 1H), 1.91-1.60 (m, 7H), 1.41-1.15 (m, 4H), 1.12-0.75 (m, 4H), 0.54-0.40 (m, 2H); MS (ESI) m/z 662.1 (M+H)

S25-11-142:

¹H NMR (400 MHz, CD₃OD) δ 7.35, 7.30 (s, 1H total), 4.83-4.59 (m, 2H), 4.15 (s, 1H), 3.40-3.35 (m, 1H), 3.24-2.93 (m, 12H), 2.68-2.61 (m, 1H), 2.29-2.25 (m, 1H), 2.08-1.92 (m, 1H), 1.85-1.42 (m, 11H), 1.35-1.16 (m, 3H), 0.86-0.78 (m, 2H), 0.54-0.41 (m, 2H); MS (ESI) m/z 676.1 (M+H)

S25-11-143:

¹H NMR (400 MHz, CD₃OD) δ 7.31 (s, 1H), 4.82-4.71 (m, 1H), 4.58-4.42 (m, 1H), 4.14 (s, 1H), 4.11-4.01 (m, 1H), 3.21-2.97 (m, 11H), 2.68-2.59 (m, 1H), 2.48-2.33 (m, 2H), 2.28-2.20 (m, 1H), 2.18-1.60 (m, 5H), 1.21-1.10 (m, 1H), 0.85-0.74 (m, 2H), 0.48-0.35 (m, 2H); MS (ESI) m/z 620.1 (M+H).

S25-11-144:

¹H NMR (400 MHz, CD₃OD) δ 7.29, 7.25 (s, 1H total), 4.68-4.62 (m, 1H), 4.36-4.30 (m, 1H), 4.12 (s, 1H), 3.15-2.93 (m, 12H), 2.70-2.60 (m, 1H), 2.28-2.22 (m, 1H), 1.70-1.60 (m, 1H), 1.51 (d, J=6.4 Hz, 3H), 0.97-0.73 (m, 6H), 0.67-0.38 (m, 6H); MS (ESI) m/z 634.1 (M+H)

S25-11-145:

¹H NMR (400 MHz, CD₃OD) δ 7.86-7.72 (m, 2H), 7.57-7.43 (m, 3H), 7.22, 7.08 (s, 1H total), 4.64-4.36 (m, 2H), 4.12 (s, 1H), 3.73-3.61 (m, 1H), 3.23-2.89 (m, 10H), 2.66-2.48 (m, 1H), 2.30-2.20 (m, 1H), 2.04-1.99 (m, 6H), 1.65-1.56 (m, 1H), 1.19, 0.98 (t, J=7.2 Hz, 3H total); MS (ESI) m/z 658.1 (M+H).

S25-11-146:

¹H NMR (400 MHz, CD₃OD) δ 7.37 (s, 1H), 5.10 (d, J=14.0 Hz, 1H), 4.75 (d, J=14.0 Hz, 1H), 4.15 (s, 1H), 4.10-4.08 (m, 2H), 3.30-2.96 (m, 13H), 2.69-2.58 (m, 1H), 2.27-2.23 (m, 1H), 1.69-1.60 (m, 1H), 1.02 (s, 9H); MS (ESI) m/z 626.1 (M+H).

S25-11-147:

¹H NMR (400 MHz, CD₃OD) δ 7.27 (s, 1H), 4.60-4.42 (m, 2H), 4.14 (s, 1H), 3.22-2.90 (m, 12H), 2.70-2.62 (m, 1H), 2.27-2.23 (m, 1H), 1.70-1.60 (m, 1H), 1.59 (s, 3H), 1.31-1.22 (m, 2H), 1.08-0.87 (m, 2H); MS (ESI) m/z 580.1 (M+H).

S25-11-148:

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.83-4.66 (m, 2H), 4.13 (s, 1H), 3.51-3.40 (m, 2H), 3.21-2.96 (m, 9H), 2.69-2.58 (m, 1H), 2.26-2.22 (m, 1H), 1.69-1.60 (m, 1H), 1.59 (s, 3H), 1.48-1.35 (m, 4H), 1.05-0.76 (m, 3H); MS (ESI) m/z 594.1 (M+H).

S25-11-149:

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.56-4.52 (m, 1H), 4.18-4.13 (m, 2H), 3.21-2.97 (m, 9H), 2.66 (s, 3H), 2.65-2.45 (m, 3H), 2.29-2.17 (m, 2H), 2.11-2.02 (m, 1H), 1.96-1.90 (m, 2H), 1.69-1.60 (m, 4H); MS (ESI) m/z 594.1 (M+H).

S-25-11-149:

¹H NMR (400 MHz, CD₃OD) δ 7.36, 7.35 (s, 1H total), 4.53-4.36 (m, 2H), 4.14 (s, 1H), 3.21-2.96 (m, 11H), 2.76-2.47 (m, 3H), 2.36-2.22 (m, 1H), 2.13-2.02 (m, 2H), 2.01-1.88 (m, 2H), 1.68-1.63 (m, 4H), 1.23, 1.12 (t, J=7.2 Hz, 3H total); MS (ESI) m/z 608.1 (M+H).

S25-11-151:

¹H NMR (400 MHz, CD₃OD) δ 7.67-7.27 (m, 5H), 6.88 (s, 1H), 4.11 (s, 1H), 4.05 (d, J=13.6 Hz, 1H), 3.96 (d, J=14.0 Hz, 1H), 3.21-2.85 (m, 9H), 2.57-2.50 (m, 1H), 2.23-2.13 (m, 1H), 1.90 (m, 6H), 1.64-1.54 (m, 1H); MS (ESI) m/z 630.1 (M+H).

S25-11-152:

¹H NMR (400 MHz, CD₃OD) δ 7.53 (s, 5H), 7.21 (s, 1H), 4.75-4.62 (m, 1H), 4.53-4.27 (m, 3H), 4.13 (s, 1H), 3.20-2.85 (m, 9H), 2.79 (s, 3H), 2.69-2.57 (m, 1H), 2.25-2.14 (m, 1H), 1.68-1.58 (m, 1H); MS (ESI) m/z 616.1 (M+H).

S25-11-153:

¹H NMR (400 MHz, CD₃OD) δ 7.73-7.54 (m, 5H), 7.23, 7.18, 7.13 (s, 1H total), 4.87-4.73 (m, 2H), 4.13-4.02 (m, 2H), 3.21-2.88 (m, 9H), 2.79, 2.72 (s, 3H total), 2.66-2.52 (m, 1H), 2.30-2.21 (m, 1H), 1.86 (d, J=4.4 Hz, 3H), 1.67-1.58 (m, 1H); MS (ESI) m/z 630.0 (M+H).

S25-11-154:

¹H NMR (400 MHz, CD₃OD) δ 7.64-7.50 (m, 5H), 7.16, 7.08 (s, 1H total), 5.02-4.98 (m, 1H), 4.68-4.23 (m, 2H), 4.13 (s, 1H), 3.47-3.35 (m, 1H), 3.21-2.88 (m, 10H), 2.69-2.54 (m, 1H), 2.26-2.20 (m, 1H), 1.88-1.83 (m, 3H), 1.66-1.57 (m, 1H), 1.44-1.33 (m, 3H); MS (ESI) m/z 644.2 (M+H).

S25-11-155:

¹H NMR (400 MHz, CD₃OD) δ 7.17 (s, 1H), 4.21 (d, J=14.4 Hz, 1H), 4.31 (d, J=14.0 Hz, 1H), 4.14 (s, 1H), 3.22-2.88 (m, 11H), 2.69-2.57 (m, 1H), 2.26-2.23 (m, 1H), 1.68-1.59 (m, 1H), 1.38 (t, J=7.2 Hz, 3H); MS (ESI) m/z 540.1

S25-11-156:

¹H NMR (400 MHz, CD₃OD) δ 7.60-7.45 (m, 5H), 7.21, 7.18 (s, 1H total), 5.10-4.93 (m, 1H), 4.72-4.64 (m, 1H), 4.49-4.45 (1H), 4.12 (s, 1H), 3.22-2.87 (m, 11H), 2.66-2.55 (m, 1H), 2.26-2.18 (m, 1H), 1.95-1.85 (m, 3H), 1.68-1.58 (m, 1H), 1.15-1.08 (m, 1H), 0.90-0.66 (m, 2H), 0.50-0.35 (m, 2H); MS (ESI) m/z 670.1 (M+H).

S25-11-157:

¹H NMR (400 MHz, CD₃OD) δ 7.65-7.48 (m, 5H), 7.26, 7.10 (s, 1H total), 5.35-5.25 (m, 0.5H), 4.76-4.65 (m, 1.5H), 4.49-4.35 (m, 1H), 4.11 (s, 1H), 3.21-2.95 (m, 11H), 2.66-2.54 (m, 1H), 2.26-2.19 (m, 1H), 1.95-1.82 (m, 3H), 1.68-1.59 (m, 1H), 1.20-1.01 (m, 1H), 0.91-0.68 (m, 2H), 0.48-0.33 (m, 2H); MS (ESI) m/z 670.1 (M+H).

S25-11-158:

¹H NMR (400 MHz, CD₃OD) δ 7.27 (s, 1H), 6.39 (t, J=54.0 Hz, 1H), 4.57 (d, J=13.6 Hz, 1H), 4.46 (d, J=13.2 Hz, 1H), 4.14 (s, 1H), 3.67 (t, J=14.4 Hz, 2H), 3.15-2.98 (m, 9H), 2.89 (s, 3H), 2.68-2.60 (m, 1H), 2.28-2.23 (m, 1H), 1.69-1.62 (m, 1H); MS (ESI) m/z 590.1 (M+H).

S25-11-159:

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.52 (d, J=14.4 Hz, 1H), 4.43 (d, J=14.4 Hz, 1H), 4.14 (s, 1H), 4.11-4.04 (m, 2H), 3.15-2.90 (m, 9H), 2.68-2.60 (m, 1H), 2.27-2.24 (m, 1H), 1.68-1.62 (m, 1H); MS (ESI) m/z 594.1 (M+H).

S25-11-160:

¹H NMR (400 MHz, CD₃OD) δ 7.80-7.78 (m, 2H), 7.64-7.29 (m, 3H), 7.01, 6.93 (s, 1H total), 4.52-4.41 (m, 1H), 4.15 (s, 1H), 4.13-4.08 (m, 1H), 3.23-2.87 (m, 9H), 2.77 (s, 3H), 2.61-2.48 (m, 1H), 2.25-2.20 (m, 1H), 2.05 (m, 6H), 1.66-1.57 (m, 1H); MS (ESI) m/z 644.1 (M+H).

S25-11-161:

¹H NMR (400 MHz, CD₃OD) δ 7.41, 7.38, 7.23 (s, 1H total), 4.76-4.45 (m, 2H), 4.16, 4.15 (s, 1H total), 3.62-3.35 (m, 2H), 3.26-2.96 (m, 10H), 2.70-2.64 (m, 1H), 2.28-2.24 (m, 1H), 1.67-1.55 (m, 3H), 1.48-1.38 (m, 3H), 1.38-1.28 (m, 1H), 1.24 (s, 3H), 0.92 (s, 6H); MS (ESI) m/z 624.1 (M+H).

S25-11-162:

¹H NMR (400 MHz, CD₃OD) δ 7.24, 7.23 (s, 1H total), 4.43 (d, J=13.2 Hz, 1H), 4.30 (d, J=13.6 Hz, 1H), 4.14 (s, 1H), 3.21-2.88 (m, 9H), 2.69-2.58 (m, 1H), 2.27-2.23 (m, 1H), 2.03-1.69 (m, 8H), 1.69-1.61 (m, 1H), 1.49 (s, 3H); MS (ESI) m/z 594.1 (M+H).

S25-11-163:

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.43 (d, J=13.6 Hz, 1H), 4.28 (d, J=12.8 Hz, 1H), 4.14 (s, 1H), 3.11-2.96 (m, 9H), 2.66-2.58 (m, 1H), 2.26-2.23 (m, 1H), 2.01-1.94 (m, 2H), 1.78-1.53 (m, 9H), 1.50 (s, 3H); MS (ESI) m/z 608.1 (M+H).

S25-11-164:

¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.43 (d, J=14.0 Hz, 1H), 4.33 (d, J=14.8 Hz, 1H), 4.10 (s, 1H), 3.03-2.95 (m, 11H), 2.69-2.57 (m, 1H), 2.26-2.22 (m, 1H), 1.83-1.76 (m, 6H), 1.39-1.25 (m, 4H), 1.11-1.05 (m, 2H); MS (ESI) m/z 608.1 (M+H).

S25-11-165:

¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.45-4.28 (m, 2H), 4.13 (s, 1H), 3.21-2.96 (m, 10H), 2.66-2.60 (m, 1H), 2.26-2.15 (m, 2H), 1.95-1.84 (m, 3H), 1.78-1.60 (m, 3H), 1.45-1.21 (m, 6H); MS (ESI) m/z 594.1 (M+H).

S25-11-166:

¹H NMR (400 MHz, CD₃OD) δ 7.17 (s, 1H), 4.48 (d, J=14.4 Hz, 1H), 4.30 (d, J=14.0 Hz, 1H), 4.04 (s, 1H), 3.48 (t, J=12.4 Hz, 2H), 3.17-2.86 (m, 11H), 2.60-2.50 (m, 1H), 2.17-2.13 (m, 1H), 1.92-1.85 (m, 2H), 1.60-1.51 (m, 2H), 1.43-1.15 (m, 6H), 0.82 (t, J=7.2 Hz, 3H); MS (ESI) m/z 622.2 (M+H).

S25-11-211:

MS (ESI) m/z 650.1 (M+H).

S25-11-168:

¹H NMR (400 MHz, CD₃OD) δ 7.28 (s, 1H), 4.64 (d, J=14.4 Hz, 1H), 4.49 (d, J=13.6 Hz, 1H), 4.13 (s, 1H), 4.05-3.75 (m, 4H), 3.51-3.40 (m, 4H), 3.15-2.96 (m, 9H), 2.69-2.60 (m, 1H), 2.26-2.22 (m, 1H), 1.68-1.62 (m, 1H); MS (ESI) m/z 582.2 (M+H).

S25-11-169:

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.57 (d, J=13.6 Hz, 1H), 4.39 (d, J=13.6 Hz, 1H), 4.13 (s, 1H), 3.56 (t, J=14.4 Hz, 2H), 3.23-2.95 (m, 11H), 2.66-2.59 (m, 1H), 2.26-2.23 (m, 1H), 1.98-1.90 (m, 3H), 1.70-1.60 (m, 3H), 1.55-1.45 (m, 2H), 1.01 (d, J=6.4 Hz, 3H); MS (ESI) m/z 594.2 (M+H).

S25-11-170:

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.44 (q, J=14 Hz, 2H), 4.15 (s, 1H), 3.05-2.96 (m, 9H), 2.81-2.79 (m, 1H), 2.64-2.60 (m, 1H), 2.27-2.23 (m, 1H), 1.63 (q, J=13.2 Hz, 1H), 1.49 (d, J=6.4 Hz, 3H), 1.11-1.05 (m, 1H), 0.83-0.81 (m, 2H), 0.62-0.59 (m, 1H), 0.39-0.35 (m, 1H); MS (ESI) m/z 580.2 (M+H).

S25-11-171:

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.46 (d, J=14 Hz, 1H), 4.34 (d, J=13.2 Hz, 1H), 4.15 (s, 1H), 3.36-3.37 (m, 1H), 3.05-2.96 (m, 9H), 2.65-2.56 (m, 1H), 2.27-2.21 (m, 2H), 1.68-1.59 (m, 1H), 1.32 (d, J=6.8 Hz, 3H), 1.05 (d, J=6.8 Hz, 3H), 1.01 (d, J=6.8 Hz, 3H); MS (ESI) m/z 582.3 (M+H).

S25-11-172:

¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.43 (d, J=14.4 Hz, 1H), 4.33 (d, J=14 Hz, 1H), 4.13 (s, 1H), 3.15-2.96 (m, 10H), 2.65-2.61 (m, 2H), 2.29-2.23 (m, 2H), 1.96-1.93 (m, 2H), 1.74-1.63 (m, 5H), 1.31-1.29 (m, 2H); MS (ESI) m/z 594.5 (M+H).

S25-11-173:

¹H NMR (400 MHz, CD₃OD) δ 7.27 (s, 1H), 5.49 (d, J=52.8 Hz, 1H), 4.55 (m, 1H), 4.12 (s, 1H), 3.95-2.43 (m, 4H), 3.04-2.96 (m, 9H), 2.69-2.61 (m, 3H), 2.41-2.23 (m, 1H), 2.25-2.22 (m, 1H), 1.69-1.58 (m, 1H); MS (ESI) m/z 584.3 (M+H).

S25-11-174:

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 5.49 (d, J=52.8 Hz, 1H), 4.59 (d, J=13.6 Hz, 1H), 4.13 (s, 1H), 3.83-2.79 (m, 4H), 3.04-2.96 (m, 9H), 2.65-2.62 (m, 3H), 2.44-2.43 (m, 1H), 2.26-2.22 (m, 1H), 1.69-1.59 (m, 1H); MS (ESI) m/z 584.2 (M+H).

S25-11-175:

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.54 (d, J=13.2 Hz, 1H), 4.59 (d, J=13.2 Hz, 1H), 4.11 (s, 1H), 3.61-3.53 (m, 2H), 3.39-3.34 (m, 6H), 3.28-2.81 (m, 9H), 2.67-2.57 (m, 1H), 2.24-1.92 (m, 5H), 1.68-1.58 (m, 1H); MS (ESI) m/z 610.0 (M+H).

S25-11-176:

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.56 (d, J=14.4 Hz, 1H), 4.42 (d, J=14.4 Hz, 1H), 4.12 (s, 1H), 3.89-3.38 (m, 4H), 3.25-2.91 (m, 9H), 2.65-2.57 (m, 1H), 2.24-2.21 (m, 1H), 2.12-1.74 (m, 5H), 1.68-1.58 (m, 1H); MS (ESI) m/z 596.0 (M+H).

S25-11-177:

¹H NMR (400 MHz, CD₃OD) δ 7.29 (s, 1H), 4.61 (d, J=13.6 Hz, 1H), 4.42 (d, J=13.6 Hz, 1H), 4.13 (s, 1H), 3.74-3.61 (m, 2H), 3.29-3.15 (m, 2H), 3.04-2.95 (m, 9H), 2.69-2.59 (m, 3H), 2.25-2.15 (m, 4H), 1.69-1.59 (m, 1H); MS (ESI) m/z 648.0 (M+H).

S25-11-178:

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.41 (d, J=13.6 Hz, 1H), 4.26 (d, J=13.6 Hz, 1H), 4.14 (s, 1H), 3.09-2.96 (m, 9H), 2.69-2.58 (m, 1H), 2.25-2.22 (m, 1H), 1.81 (s, 2H), 1.68-1.59 (m, 1H), 1.57 (s, 6H), 1.11 (s, 9H); MS (ESI) m/z 624.1 (M+H).

S25-11-179:

¹H NMR (400 MHz, CD₃OD) δ 7.14 (s, 1H), 4.60 (d, J=13.6 Hz, 1H), 4.36 (d, J=13.2 Hz, 1H), 4.05 (s, 1H), 3.77-3.70 (m, 2H), 3.22-2.79 (m, 13H), 2.59-2.49 (m, 1H), 2.17-2.14 (m, 1H), 1.85-1.50 (m, 7H); MS (ESI) m/z 606.0 (M+H)

S25-11-180:

¹H NMR (400 MHz, CD₃OD) δ 7.29 (s, 1H), 4.41-4.38 (m, 2H), 4.13 (s, 1H), 3.51-3.49 (m, 1H), 3.23-2.91 (m, 9H), 2.69-2.63 (m, 1H), 2.44-2.40 (m, 1H), 2.27-2.23 (m, 1H), 1.94-1.82 (m, 2H), 1.69-1.53 (m, 3H), 1.50-1.45 (m, 2H), 1.28 (s, 3H), 0.97 (s, 6H); MS (ESI) m/z 648.3 (M+H).

S25-11-181:

¹H NMR (400 MHz, CD₃OD) δ 7.26-7.10 (s, 1H total), 4.46 (d, J=13.2 Hz, 1H), 4.33 (d, J=14.0 Hz, 1H), 4.11 (s, 1H), 3.55 (s, 1H), 3.24-2.87 (m, 9H), 2.69-2.59 (m, 1H), 2.31-2.21 (m, 3H), 2.02-1.76 (m, 17H), 1.70-1.60 (m, 1H); MS (ESI) m/z 646.1.0 (M+H).

S25-11-182:

¹H NMR (400 MHz, CD₃OD) δ 7.11 (s, 1H), 4.34 (d, J=14.8 Hz, 1H), 4.23 (d, J=14.8 Hz, 1H), 4.05 (s, 1H), 3.63-3.60 (m, 1H), 3.18-2.88 (s, 9H), 2.60-2.50 (m, 1H), 2.17-2.05 (m, 3H), 1.80-1.71 (m, 2H), 1.69-1.61 (m, 4H), 1.59-1.52 (m, 1H); MS (ESI) m/z 580.0 (M+H).

S25-11-183:

¹H NMR (400 MHz, CD₃OD) δ 7.12, 7.10 (s, 1H total), 4.33 (d, J=14.0 Hz, 1H), 4.22 (d, J=14.4 Hz, 1H), 4.05, 4.04 (s, 1H total), 3.52-3.46 (m, 1H), 3.20-2.88 (s, 9H), 2.61-2.52 (m, 1H), 2.27-2.22 (m, 1H), 1.61-1.52 (m, 1H), 1.35 (d, J=6.4 Hz, 6H); MS (ESI) m/z 554.0 (M+H).

S25-11-184:

¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.43-4.39 (m, 1H), 4.33-4.29 (m, 1H), 4.12 (s, 1H), 3.23-2.90 (s, 11H), 2.68-2.55 (m, 1H), 2.25-2.22 (m, 1H), 2.02-1.91 (m, 1H), 1.87-1.78 (m, 2H), 1.75-1.47 (m, 9H), 1.36-1.28 (m, 2H); MS (ESI) m/z 622.0 (M+H).

S25-11-185:

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.75-4.65 (m, 1H), 4.58-4.42 (m, 1H), 4.12 (s, 1H), 3.72-3.58 (m, 1H), 3.55-3.36 (m, 2H), 3.25-2.95 (s, 10H), 2.67-2.55 (m, 1H), 2.26-2.21 (m, 1H), 2.06-1.98 (m, 1H), 1.90-1.82 (m, 1H), 1.62-1.51 (m, 5H), 0.93-0.84 (m, 6H); MS (ESI) m/z 622.0 (M+H).

S25-11-186:

¹H NMR (400 MHz, CD₃OD) δ 7.27-7.14 (m, 6H), 4.57 (d, J=14.4 Hz, 1H), 4.40 (d, J=14.4 Hz, 1H), 4.07 (s, 1H), 3.66-3.57 (m, 2H), 3.35-3.29 (m, 2H), 3.17-2.91 (s, 10H), 2.61-2.51 (m, 1H), 2.20-2.16 (m, 1H), 2.06-1.98 (m, 4H), 1.62-1.52 (m, 1H); MS (ESI) m/z 656.3 (M+H).

S25-11-187:

¹H NMR (400 MHz, CD₃OD) δ 7.18 (s, 1H), 4.48 (d, J=14.0 Hz, 1H), 4.30 (d, J=14.0 Hz, 1H), 4.05 (s, 1H), 3.56-3.49 (m, 2H), 3.23-2.88 (s, 11H), 2.62-2.52 (m, 1H), 2.18-2.14 (m, 1H), 1.92-1.85 (m, 2H), 1.59-1.39 (m, 5H), 0.85 (d, J=6.8 Hz, 6H); MS (ESI) m/z 622.1 (M+H).

S25-11-188:

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.54 (d, J=13.2 Hz, 1H), 4.38 (d, J=14.4 Hz, 1H), 4.07 (s, 1H), 3.47-3.35 (m, 4H), 3.13-2.90 (s, 9H), 2.63-2.55 (m, 1H), 2.21-2.15 (m, 1H), 1.88-1.75 (m, 4H), 1.63-1.55 (m, 1H), 1.23 (s, 3H); MS (ESI) m/z 610.1 (M+H).

S25-11-189:

¹H NMR (400 MHz, CD₃OD) δ 7.18 (s, 1H), 4.55 (d, J=12.4 Hz, 1H), 4.44 (d, J=16.0 Hz, 1H), 4.12 (s, 1H), 3.22-2.80 (m, 13H), 2.67-2.56 (m, 1H), 2.25-2.12 (m, 4H), 2.06-1.83 (m, 5H), 1.69-1.59 (m, 1H), 1.40-1.30 (m, 3H); MS (ESI) m/z 608.2 (M+H).

S25-11-190:

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.80-4.49 (m, 2H), 4.14 (s, 1H), 3.25-2.95 (m, 13H), 2.67-2.57 (m, 1H), 2.27-2.22 (m, 1H), 1.88-1.60 (m, 3H), 1.25-1.12 (m, 1H), 1.02-0.95 (m, 3H), 0.85-0.75 (m, 2H), 0.48-0.37 (m, 2H); MS (ESI) m/z 608.1 (M+H).

S25-11-191:

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.81-4.50 (m, 2H), 4.14 (s, 1H), 3.41-3.35 (m, 1H), 3.21-2.96 (m, 12H), 2.68-2.58 (m, 1H), 2.27-2.22 (m, 1H), 1.83-1.56 (m, 3H), 1.21-1.10 (m, 1H), 1.02-0.93 (m, 7H), 0.85-0.75 (m, 2H), 0.52-0.39 (m, 2H); MS (ESI) m/z 636.1 (M+H).

S25-11-192:

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.81-4.49 (m, 2H), 4.13 (s, 1H), 3.39-3.35 (m, 1H), 3.23-2.98 (m, 12H), 2.69-2.59 (m, 1H), 2.26-2.21 (m, 1H), 1.85-1.60 (m, 3H), 1.45-1.35 (m, 2H), 1.23-1.10 (m, 1H), 0.98 (d, J=7.2 Hz, 3H), 0.85-0.75 (m, 2H), 0.49-0.39 (m, 2H); MS (ESI) m/z 622.1 (M+H).

S25-11-193:

¹H NMR (400 MHz, CD₃OD) δ 7.24, 7.21 (s, 1H total), 4.60-4.44 (m, 2H), 4.13 (s, 1H), 3.22-2.91 (m, 13H), 2.69-2.59 (m, 1H), 2.29-2.09 (m, 2H), 1.69-1.60 (m, 1H), 1.45-1.35 (m, 3H), 1.09-0.95 (m, 6H); MS (ESI) m/z 596.1 (M+H).

S25-11-194:

¹H NMR (400 MHz, CD₃OD) δ 7.21 (s, 1H), 4.43 (d, J=13.8 Hz, 1H), 4.36 (d, J=14.0 Hz, 1H), 4.14 (s, 1H), 3.12-2.88 (m, 11H), 2.69-2.55 (m, 1H), 2.28-2.22 (m, 1H), 2.16-2.05 (m, 1H), 1.69-1.58 (m, 1H), 1.09 (d, J=6.8 Hz, 6H); MS (ESI) m/z 568.0 (M+H).

S25-11-195:

¹H NMR (400 MHz, CD₃OD) δ 7.25 (s, 1H), 4.47-4.37 (m, 2H), 4.13 (s, 1H), 3.28-2.94 (s, 11H), 2.67-2.57 (m, 1H), 2.27-2.22 (m, 1H), 2.00-1.89 (m, 6H), 1.67-1.61 (m, 3H), 0.91 (t, J=7.4 Hz, 3H); MS (ESI) m/z 608.0 (M+H).

S25-11-196:

¹H NMR (400 MHz, CD₃OD) δ 7.27 (s, 1H), 4.68-4.61 (m, 2H), 4.59-4.51 (m, 1H), 4.41-4.39 (m, 1H), 4.15 (s, 1H), 3.99-3.87 (m, 1H), 3.58-3.52 (m, 2H), 3.22-2.97 (m, 9H), 2.67-2.60 (m, 2H), 2.27-2.24 (m, 1H), 2.16-2.11 (m, 2H), 2.01-1.95 (m, 3H), 1.71-1.61 (m, 1H); MS (ESI) m/z 623.0 (M+H).

S25-11-197:

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.56-4.51 (m, 2H), 4.47-4.32 (m, 2H), 4.14 (s, 1H), 3.94-3.72 (m, 2H), 3.65-3.52 (m, 2H), 3.22-2.97 (m, 9H), 2.67-2.62 (m, 2H), 2.27-2.24 (m, 1H), 2.01-1.98 (m, 4H), 1.71-1.61 (m, 1H); MS (ESI) m/z 623.0 (M+H).

S25-11-198:

¹H NMR (400 MHz, CD₃OD) δ 7.30 (s, 1H), 4.67-4.62 (m, 1H), 4.49-4.42 (m, 1H), 4.05 (s, 1H), 3.56-3.52 (m, 1H), 3.44-3.31 (m, 1H), 3.05-2.88 (m, 9H), 2.58-2.45 (m, 2H), 2.35-2.31 (m, 1H), 2.18-2.15 (m, 1H), 1.69-1.21 (m, 11H); MS (ESI) m/z 620.0 (M+H).

S25-11-199:

¹H NMR (400 MHz, CD₃OD) δ 7.19 (s, 1H), 4.67-4.63 (m, 1H), 4.50-4.46 (m, 1H), 4.06 (s, 1H), 3.89-3.82 (m, 2H), 3.68-3.62 (m, 2H), 3.16-2.80 (m, 9H), 2.69-2.48 (m, 3H), 2.18-2.11 (m, 1H), 1.60-1.50 (m, 1H); MS (ESI) m/z 601.9 (M+H).

S25-11-200:

¹H NMR (400 MHz, CD₃OD) δ 7.28 (s, 1H), 4.61-4.56 (m, 1H), 4.41-4.38 (m, 1H), 4.14 (s, 1H), 3.66-3.61 (m, 2H), 3.19-2.99 (m, 11H), 2.67-2.60 (m, 1H), 2.28-2.24 (m, 1H), 2.03-1.95 (m, 2H), 1.71-1.61 (m, 3H), 1.48-1.41 (m, 1H), 0.93 (s, 9H); MS (ESI) m/z 636.1 (M+H).

S25-11-201:

¹H NMR (400 MHz, CD₃OD) δ 7.47-7.26 (m, 5H), 4.68-4.58 (m, 3H), 4.18 (s, 1H), 3.56-3.45 (m, 4H), 3.29-2.99 (m, 11H), 2.69-2.61 (m, 1H), 2.30-2.27 (m, 1H), 1.72-1.63 (m, 1H), 1.21-1.11 (m, 1H), 0.82-0.77 (m, 2H), 0.43-0.38 (m, 2H); MS (ESI) m/z 682.3 (M+H).

S25-11-202:

¹H NMR (400 MHz, CD₃OD) δ 7.36 (s, 1H), 4.70-4.63 (m, 2H), 4.18 (s, 1H), 3.53-3.48 (m, 1H), 3.31-2.94 (m, 12H), 2.69-2.57 (m, 2H), 2.48-2.45 (m, 2H), 2.32-2.25 (m, 1H), 2.05-1.98 (m, 6H), 1.70-1.67 (m, 1H), 1.26-1.22 (m, 2H), 1.18-1.14 (m, 1H), 1.10-1.04 (m, 2H), 0.88-0.84 (m, 3H), 0.72-0.70 (m, 1H), 0.54-0.49 (m, 2H); MS (ESI) m/z 702.3 (M+H).

S25-11-203:

¹H NMR (400 MHz, CD₃OD) δ 7.39, 7.29 (s, 1H total), 4.78-4.65 (m, 1H), 4.36-4.34 (m, 1H), 3.91-3.85 (m, 1H), 3.46-3.40 (m, 1H), 4.18 (s, 1H), 3.31-2.98 (m, 11H), 2.68-2.63 (m, 1H), 2.32-2.25 (m, 1H), 1.72-1.65 (m, 1H), 1.46-1.41 (m, 3H), 1.21-1.15 (m, 3H), 1.08-1.02 (m, 4H), 0.91-0.71 (m, 2H), 0.60-0.40 (m, 2H); MS (ESI) m/z 636.0 (M+H).

S25-11-204:

¹H NMR (400 MHz, CD₃OD) δ 7.27 (s, 1H), 4.69-4.50 (m, 2H), 4.41-4.38 (m, 1H), 4.24-4.10 (m, 3H), 3.84-3.68 (m, 3H), 3.21-2.96 (m, 10H), 2.66-2.59 (m, 1H), 2.42-2.33 (m, 2H), 2.27-2.24 (m, 1H), 1.69-1.63 (m, 1H), 1.21-1.08 (m, 1H), 0.87-0.73 (m, 2H), 0.48-0.36 (m, 2H); MS (ESI) m/z 636.2 (M+H).

S25-11-205:

¹H NMR (400 MHz, CD₃OD) δ 7.53 (s, 1H), 5.07-5.03 (m, 1H), 4.61-4.58 (m, 1H), 4.17 (s, 1H), 4.07-4.03 (m, 1H), 3.21-2.69 (m, 11H), 2.49-2.42 (m, 1H), 2.31-2.27 (m, 1H), 2.02-1.62 (m, 8H), 1.33-1.28 (m, 3H), 1.07-0.99 (m, 7H), 0.97-0.91 (m, 2H), 0.79-0.68 (m, 2H); MS (ESI) m/z 702.4 (M+H).

S25-11-206:

¹H NMR (400 MHz, CD₃OD) δ 7.22 (s, 1H), 4.46-4.43 (m, 1H), 4.37-4.34 (m, 1H), 4.15 (s, 1H), 3.24-2.99 (m, 11H), 2.67-2.46 (m, 3H), 2.28-2.25 (m, 1H), 2.18-1.98 (m, 6H), 1.69-1.62 (m, 2H), 1.28 (s, 3H), 1.07 (s, 3H); MS (ESI) m/z 648.3 (M+H).

S25-11-207:

¹H NMR (400 MHz, CD₃OD) δ 7.26 (s, 1H), 4.73-4.69 (m, 1H), 4.58-4.55 (m, 1H), 4.16 (s, 1H), 4.01-3.89 (m, 2H), 3.20-2.98 (m, 10H), 2.67-2.57 (m, 1H), 2.28-2.25 (m, 1H), 1.69-1.62 (m, 1H), 1.11 (s, 9H); MS (ESI) m/z 612.3 (M+H).

S25-11-208:

¹H NMR (400 MHz, CD₃OD) δ 7.18 (s, 1H), 4.48 (d, J=12.0 Hz, 1H), 4.33 (d, J=14.0 Hz, 1H), 4.14 (s, 1H), 3.22-2.89 (m, 11H), 2.70-2.59 (m, 1H), 2.26-2.20 (m, 1H), 1.85-1.75 (m, 3H), 1.72-1.60 (m, 2H), 1.11 (t, J=7.2 Hz, 3H); MS (ESI) m/z 554.1 (M+H).

S25-11-209:

¹H NMR (400 MHz, CD₃OD) δ 7.43, 7.39 (s, 1H total), 4.85-4.80 (m, 0.5H), 4.71-4.65 (m, 1.5H), 4.16 (s, 1H), 3.24-2.97 (m, 13H), 2.67-2.61 (m, 1H), 2.31-2.25 (m, 1H), 2.15-1.62 (m, 8H), 1.40-1.15 (m, 1H), 0.93-0.75 (m, 6H), 0.58-0.45 (m, 2H); MS (ESI) m/z 662.1 (M+H).

S25-11-210:

¹H NMR (400 MHz, CD₃OD) δ 7.24 (s, 1H), 4.76-4.64 (m, 1H), 4.46-4.38 (m, 1H), 4.12 (s, 1H), 3.45-3.42 (m, 1H), 3.18-2.94 (m, 11H), 2.65-2.58 (m, 1H), 2.33-2.19 (m, 2H), 1.67-1.57 (m, 1H), 1.36-1.22 (m, 6H), 1.10-1.07 (m, 6H); MS (ESI) m/z 610.1 (M+H).

Example 26. Synthesis of Compounds Via Scheme 26

The following compounds were prepared according to Scheme 25.

To a solution of compound S25-5 (3.0 g, 7.06 mmol, 1.0 eq.) in MeOH (50 mL), was added trimethyl orthoformate (3.74 g, 7.06 mmol, 5.0 eq.) and p-TSA (67 mg, 0.353 mmol, 0.05 eq.). The resulting mixture was refluxed overnight. The solvent was evaporated, the residue was diluted with EtOAc (50 mL), the resulting solution was washed with sat. aq. NaHCO₃/brine (1:1, 50 mL). The organic phase was dried over NaSO₄, filtered and concentrated. The residue was purified with flash chromatography (200˜300 mesh, PE/EA=100:1˜50:1) to give the desired product S26-1 (3.3 g, 99%) as a white solid: ¹H NMR (400 MHz, CDCl₃): δ 7.43 (d, J=6.8 Hz, 2H), 7.37-7.30 (m, 6H), 7.25-7.19 (m, 1H), 7.07 (d, J=6.8 Hz, 2H), 5.58 (s, 1H), 5.18 (s, 2H), 3.36 (s, 6H), 2.51 (s, 3H).

To a solution of S26-1 (471 mg, 1 mmol, 1.0 eq.) in anhydrous THF (5 mL) was added n-BuLi (2.5 M in hexane, 0.44 mL, 1.1 mmol, 1.1 eq.) slowly at −78° C. After stirred at −78° C. for 30 min, triisopropyl borate (0.46 ml, 2.0 mmol, 2.0 eq.) was added. After stirred at −78° C. for 4 h, acetic acid (0.165 mL, 3 mmol, 3.0 eq.) was added. while the resulting mixture was warmed up to 0° C., 30% H₂O₂ (0.27 mL, 2 mmol, 2.0 eq.) was added. The resulting mixture was stirred at rt overnight and then quenched with saturated aqueous NaHSO₃ solution (caution: till the starch iodide was negative). The resulting solution was extracted with EtOAc (50 mL). The organic phase was washed with saturated aqueous NaHCO₃ (30 mL), H₂O (30 mL), brine (30 mL) and dried over NaSO₄ and filtered and then concentrated to give the crude product S26-2, which was used for next step directly.

To a solution of S26-2 (1.0 mmol, 1.0 eq.) and K₂CO₃ (276 g, 2 mmol, 2.0 eq.) in MeCN (10 mL) was added 2-iodopropane (0.615 g, 5 mmol, 5.0 eq.). The resulting mixture was stirred at 80° C. overnight. The reaction mixture was filtered, the filtrate was concentrated. The residue was diluted with EtOAc (50 mL) and H₂O (20 mL). The organic phase was separated, dried over Na₂SO₄, filtered and concentrated. The residue was purified with flash chromatography (200˜300 mesh, PE/EA=100:1˜50:1) to give the desired product S27-3 (140 mg, 34.6%) as a pale yellow solid. ¹H NMR (400 MHz, CDCl₃): δ 10.30 (s, 1H), 7.38-7.36 (m, 2H), 7.33-7.27 (m, 6H), 7.20-7.17 (m, 1H), 7.02-7.00 (m, 2H), 5.10 (s, 2H), 4.12-4.08 (m, 1H), 2.34 (s, 3H), 1.29 (d, J=6.4 Hz, 6H).

To a solution of compound S26-3 (0.25 g, 0.62 mmol, 1.0 eq.) in MeOH (20 mL), was added trimethyl orthoformate (0.33 g, 3.11 mmol, 5.0 eq.) and p-TSA (6 mg, 0.031 mmol, 0.05 eq.). The resulting mixture was refluxed overnight. The solvent was evaporated, the residue was diluted with EtOAc (50 mL) and saturated NaHCO₃/brine (1:1, 20 mL). The organic phase was separated, dried over Na₂SO₄, filtered and concentrated. The residue was purified by Prep-TLC to give the desired product S26-4 (200 mg, 72%) as a colorless oil: ¹H NMR (400 MHz, CD₃OD): δ 7.45 (d, J=7.2 Hz, 2H), 7.37-7.22 (m, 6H), 7.11 (s, 1H), 7.05 (d, J=7.6 Hz, 2H), 5.54 (s, 1H), 5.15 (s, 2H), 4.27-4.21 (m, 1H), 3.32 (s, 6H), 2.33 (s, 3H), 1.29 (d, J=6.0 Hz, 6H).

To a solution of diisopropylamine (0.17 mL, 1.2 mmol, 3.0 eq.) and TMEDA (0.3 mL, 2.0 mmol, 5.0 eq.) in THF (3 mL) was added n-BuLi in hexane (2.5 M, 0.48 mL, 1.2 mmol, 3.0 eq.) dropwise at −78° C. After stirred at −78° C. for 30 min, A solution of S26-4 (180 mg, 0.4 mmol, 1.0 eq.) in THF (2.0 mL) was added. The resulting dark red solution was then stirred at −78° C. for 30 min and then cooled to −100° C., A solution of enone (173 mg, 0.36 mmol, 0.9 eq.) in THF (2.0 mL) was added. The resulting reaction mixture was warmed up to −10° C. naturally over 30 min. The reaction mixture was quenched with saturated NH₄Cl (50 mL) and then extracted with EtOAc (50 mL). The organic phase was separated, dried over Na₂SO₄, filtered and concentrated. The residue was purified by Prep-TLC to afford the desired product S26-5 (120 mg, 36%) as a pale yellow solid. ¹H NMR (400 MHz, CDCl₃): δ 7.37-7.33 (m, 4H), 7.26-7.17 (m, 5H), 7.13-7.10 (m, 1H), 6.97 (s, 1H), 5.34 (s, 1H), 5.22 (s, 2H), 5.07 (dd, J=12.8, 12.4 Hz, 2H), 4.02-3.94 (m, 2H), 3.88 (d, J=10.8 Hz, 1H), 3.21 (s, 3H), 3.13 (s, 3H), 0.72-2.70 (m, 1H), 2.41-2.32 (m, 9H), 2.00-1.96 (m, 1H), 1.16 (dd, J=6.0, 6.0 Hz, 6H), 0.68 (s, 9H), 0.12 (s, 3H), 0.09 (s, 3H).

To a solution of compound S26-5 (120 mg, 0.143 mmol, 1.0 eq.) in THF (2 mL) was added 6N HCl (0.17 mL). The resulting reaction solution was stirred at rt for 1 h. The reaction mixture was diluted with EtOAc (40 mL). The resulting mixture was washed with saturated NaHCO₃ (10 mL), and then brine (10 mL). The organic phase was dried over Na₂SO₄, filtered and concentrated to give the desired crude product S26-6 as a yellow oil.

To a solution of crude compound S26-6 (0.0715 mmol, 1.0 eq.) in 1,2-dichloroethane (5 mL) were added pyrrolidine (25 μL, 0.286 mmol, 4.0 eq.) and acetic acid (13 μL, 0.215 mmol, 3.0 eq.). After stirring for 2 h at r.t., sodium triacetoxyborohydride (30 mg, 0.0.143 mmol, 2.0 eq.) was added. The resulting reaction mixture was still stirred at r.t. for 2 h. The reaction was quenched with saturated aqueous NaHCO₃ and brine (1:1, 20 mL) and then extracted with EtOAc (40 mL). The organic phase were dried over anhydrous Na₂SO₄, filtered and concentrated to give the crude product, which was used for the next step directly.

To the crude product in THF (8 mL) solution was added Aqueous HF (40%, 4 mL) in a polypropylene reaction vessel at 23° C. The reaction mixture was stirred vigorously at 23° C. overnight and poured into saturated aqueous K₂HPO₄ (150 mL). The reaction mixture was extracted with EtOAc (2×20 mL). The combined organic phases were dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was used in the next step directly without further purification.

To the crude product in HCl/MeOH (4 N, 1 mL) and MeOH (5 mL) was added Pd/C (10 wt %, 50 mg) in one portion at 23° C. The reaction vessel was sealed and purged with H₂ gas (1 atm) for 1 h. The reaction mixture was then filtered through a small Celite pad. The filtrate was concentrated. The residue was purified by preparative HPLC to yield compound S26-7-1 (13.35 mg, 33.6% for 3 steps) as a yellow solid: ¹H NMR (400 MHz, CD₃OD) δ 7.06 (s, 1H), 4.50 (d, J=12.8 Hz, 1H), 4.33 (d, J=12.8 Hz, 1H), 4.16-4.12 (m, 2H), 3.54-3.45 (m, 2H), 3.26-2.98 (m, 11H), 2.36 (dd, J=14.4, 14.0 Hz, 1H), 2.27-2.24 (m, 1H), 2.16-2.10 (m, 2H), 2.09-2.01 (m, 2H), 1.65 (ddd, J=13.2, 13.2, 13.2 Hz, 1H), 1.37 (d, J=6.0 Hz, 3H), 1.20 (d, J=6.0 Hz, 3H); MS (ESI) m/z 556.1 (M+H).

The following compounds were prepared similarly to S26-7-1.

¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.35 (d, J=14.0 Hz, 1H), 4.18-4.09 (m, 3H), 3.20-2.88 (m, 11H), 2.36 (dd, J=14.4, 14.4 Hz, 1H), 2.24 (ddd, J=13.6, 5.2, 2.8 Hz, 1H), 2.09-2.02 (m, 1H), 1.65 (ddd, J=14.0, 14.0, 14.0 Hz, 1H), 1.35 (d, J=6.0 Hz, 3H), 1.20 (d, J=6.0 Hz, 3H), 1.03 (d, J=4.4 Hz, 6H); MS (ESI) m/z 558.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.04 (s, 1H), 4.53, 4.32 (d, J=13.2 Hz, 1H total), 4.46, 4.21 (d, J=13.2 Hz, 1H total), 4.18-4.12 (m, 1H), 4.10 (s, 1H), 3.25-2.97 (m, 13H), 2.44-2.35 (m, 1H), 2.26-2.23 (m, 1H), 2.17-2.06 (m, 1H), 1.65 (ddd, J=13.2, 13.2, 13.2 Hz, 1H), 1.39-1.35 (m, 6H), 1.20 (t, J=7.2 Hz, 3H), 1.03 (d, J=4.4 Hz, 3H), 1.00-0.94 (m, 3H); MS (ESI) m/z 586.1 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.05 (s, 1H), 4.64, 4.08 (d, J=12.8 Hz, 1H total), 4.43, 4.35 (d, J=12.8 Hz, 1H total), 4.18-4.13 (m, 2H), 3.19-2.99 (m, 11H), 2.80 (s, 3H), 2.39 (dd, J=14.4, 15.2 Hz, 1H), 2.29-2.19 (m, 2H), 1.66 (ddd, J=12.4, 12.0, 12.0 Hz, 1H), 1.41-1.36 (m, 3H), 1.23-1.17 (m, 3H), 1.10-1.00 (m, 7H); MS (ESI) m/z 572.1 (M+H).

Example 27. Synthesis of Compounds Via Scheme 27

The following compounds were prepared according to Scheme 27.

Sodium borohydride (0.387 g, 10.2 mmol) was added to a suspension of S1-7 (3.02 g, 6.81 mmol) in MeOH (30 mL). After bubbling ceased and complete solution was achieved, the reaction was quenched with NaHCO₃ (saturated, aqueous solution) and was extracted with EtOAc (3×). The combined EtOAc extracts were dried over sodium sulfate and concentrated. The material was dissolved in CH₂Cl₂ (20 mL) and triphenylphosphine (2.14 g, 8.17 mmol) was added. A solution of carbontetrabromide (2.71 g, 8.17 mmol) in CH₂Cl₂ (5 mL) was added dropwise. After stirring overnight, ˜50% conversion was observed. Additional triphenylphosphine (2.14 g, 8.17 mmol) and carbontetrabromide (2.71 g, 8.17 mmol) were added. After 1 h, the reaction mixture was concentrated, and the residue was triturated with toluene (5 times). The combined toluene fractions were concentrated, and the material was purified by column chromatography (Biotage 100 g column, 2 to 20% EtOAc in Hexanes gradient). This gave 2.71 g (71%) of the product: R_(f) 0.41 (10% EtOAc/hexanes); ¹H NMR (400 MHz, CDCl₃) δ 7.50-7.46 (m, 2H), 7.40-7.32 (m, 5H), 7.29-7.22 (m, 1H), 7.04 (d, J=8.7 Hz, 2H), 5.11 (s, 2H), 4.67 (d, J=2.3 Hz, 2H), 2.35 (d, J=2.3 Hz, 3H); MS (ESI) m/z 528.95, 530.95, 532.95 (M+Na).

Sodium cyanide (0.104 g, 3.12 mmol) was added to a solution of S27-1-1 (1.08 g, 2.12 mmol) in DMF (5 mL) and water (1 mL). The reaction mixture was heated to 70° C. overnight. Upon cooling to rt, the reaction mixture was diluted with EtOAc and was washed with NaHCO₃ (saturated, aqueous solution, 2 times) water (2 times), and brine (1 time). The organics were dried over sodium sulfate, filtered and concentrated. The material was purified by column chromatography (Biotage 50 g column, 5 to 40% EtOAc in Hexanes gradient). This gave 0.82 g (85%) of the product as a white solid: R_(f) 0.33 (20% EtOAc/hexanes); MS (ESI) m/z 476.05, 478.04 (M+Na).

A solution of S27-2-1 (0.817 g, 1.80 mmol) was added to a suspension of sodium hydride (60% dispersion in mineral oil, 0.158 g, 3.96 mmol) in DMF (5 mL). After bubbling ceased (˜5 min), iodomethane (0.247 mL, 3.96 mmol) was added. After 45 min, the reaction mixture was quenched with NH₄Cl (saturated, aqueous solution) and was diluted with EtOAc. The layers were separated, and washed with water (3 times) and brine (1 time). The organics were dried over Na₂SO₄, filtered, and concentrated under reduced pressure. The material was purified by column chromatography (Biotage 50 g column, 5 to 40% EtOAc in Hexanes gradient). This gave 0.34 g (390/o) of the product: R_(f) 0.53 (20% EtOAc/hexanes); ¹H NMR (400 MHz, CDCl₃) δ 7.50-7.44 (m, 2H), 7.40-7.30 (m, 5H), 7.29-7.22 (m, 1H), 7.03 (d, J=8.2 Hz, 2H), 5.10 (s, 2H), 2.33 (d, J=2.8 Hz, 3H), 2.02 (d, J=4.1 Hz, 6H); MS (ESI) m/z 504.06, 506.06 (M+Na).

Compound S27-3-1 (0.927 g, 1.92 mmol) and mercury (II) acetate (609 mg, 1.92 mmol) were heated to 100° C. in acetic acid (25 mL). After heating overnight, the reaction mixture was heated to 120° C. After 8 h, heating continued overnight at 100° C. Upon cooling to rt, the reaction mixture was poured into ice water (100 mL). The resulting solid was collected by filtration and was washed with water (3 times). The solid was dissolved in CH₂Cl₂ and was dried over Na₂SO₄, filtered, and concentrated under reduced pressure. This gave 930 mg (97%) of the crude product as a white solid: ¹H NMR (400 MHz, CDCl₃) δ 7.50-7.42 (m, 2H), 7.40-7.32 (m, 5H), 7.28-7.24 (m, 1H), 7.03 (d, J=8.2 Hz, 2H), 5.55-5.35 (br s, 2H), 5.08 (s, 2H), 2.33 (d, J=2.7 Hz, 3H), 1.79 (d, J=5.0 Hz, 6H); MS (ESI) m/z 522.19, 524.19 (M+Na).

Bis-(trifluoroacetoxy)iodobenzene (178 mg, 0.414 mmol) was added to a solution of S27-4-1 (207 mg, 0.414 mmol) in acetonitrile (0.5 mL) and water (0.5 mL). After stirring overnight, the reaction mixture was diluted with water (5 mL) and was stirred for 30 min. The reaction mixture was diluted with NaHCO₃ (saturated, aqueous solution) and was extracted with EtOAc (3 times). The combined extracts were dried over Na₂SO₄, filtered, and concentrated under reduced pressure, yielding S27-5-1 which was used in the next step without purification: ¹H NMR (400 MHz, CDCl₃) δ 7.52-7.48 (m, 2H), 7.40-7.30 (m, 5H), 7.28-7.24 (m, 1H), 7.06-7.02 (m, 2H), 5.08 (s, 2H), 2.31 (d, J=3.2 Hz, 3H), 1.74 (d, J=4.6 Hz, 6H); MS (ESI) m/z 472.19, 474.19 (M+H).

Prepared according to the methods of compound S27-5-1, starting from compound S15-8: ¹H NMR (400 MHz, CDCl₃) δ 7.46-7.40 (m, 2H), 7.40-7.30 (m, 5H), 7.28-7.20 (m, 2H), 7.12-7.08 (m, 2H), 5.16 (s, 2H), 2.48 (s, 3H), 2.04 (br s, 2H), 1.61 (s, 6H); MS (ESI) m/z 410.39 (M+H).

Isobutyraldehyde (0.029 mL, 0.32 mmol), acetic acid (0.036 mL, 0.64 mmol) and S27-5-1 (100 mg, 0.212 mmol) were stirred in 1,2-dichloroethane (2 mL). After 1 h, sodium triacetoxyborohydride (67.4 mg, 0.318 mmol) was added. After 1 h, the reaction mixture was diluted with NaHCO₃ (saturated, aqueous solution) and was extracted with CH₂Cl₂. The combined extracts were dried over Na₂SO₄, filtered, and concentrated under reduced pressure. The material was purified by column chromatography (Biotage 10 g column, 0 to 4% MeOH in CH₂Cl₂ gradient). This gave 77.8 mg (69%) of the product (˜85% pure): R_(f) 0.38 (5% MeOH/CH₂C₂); ¹H NMR (400 MHz, CDCl₃) δ 7.3-7.7 (m, 2H), 7.40-7.32 (m, 5H), 7.26-7.20 (m, 1H), 7.03 (d, J=7.8 Hz, 2H), 5.08 (s, 2H), 2.31 (d, J=3.2 Hz, 3H), 2.00 (d, J=6.9 Hz, 2H), 1.69 (d, J=6.4 Hz, 6H), 1.68-1.58 (m, 1H), 0.86 (d, J=6.4 Hz, 6H); MS (ESI) m/z 528.26, 530.26 (M+H).

n-Butyllithium (0.127 mL, 2.2 M/hexanes, 0.279 mmol) was added to diisopropylamine (0.0395 mL, 0.279 mmol) in THF (5 mL) at −40° C. The reaction mixture was cooled to −78° C., and TMEDA (0.073 mL, 0.48 mmol) was added. A solution of compound S27-6-1 (77 mg, 0.15 mmol) in THF (1 mL) was added dropwise. Initially an orange colored solution formed, but the color faded as the solution was added. Additional lithium diisopropylamide (1.8 M solution, 0.155 mL, 0.279 mmol) was added, giving an orange color. The reaction was stirred at −78° C. for 5 min. A solution of enone S11-9 (58.4 mg, 0.121 mmol) in THF (0.5 mL) was added dropwise to the reaction mixture. The reaction was stirred from −78° C. to −20° C. for 45 minutes, quenched by saturated aqueous NH₄Cl, and extracted with EtOAc (3 times). The combined EtOAc extracts were dried (sodium sulfate) and concentrated to yield the crude product, which was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 nm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: MeOH with 0.1% HCO₂H; gradient: 80→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product were collected and concentrated to give 14 mg of compound S27-7-1 (13%): ¹H NMR (400 MHz, CDCl₃) δ 16.0-15.8 (br s, 1H), 7.58-7.45 (m, 4H), 7.40-7.20 (m, 6H), 5.36 (s, 2H), 5.00-4.85 (m, 2H), 3.93 (d, J=10.4 Hz, 1H), 3.26-3.18 (m, 1H), 3.07-2.96 (m, 1H), 2.60-2.34 (m, 9H), 2.19-2.10 (br m, 1H), 2.01 (d, J=4.9 Hz, 2H), 1.76-1.62 (m, 7H), 0.94-0.74 (m, 15H), 0.27 (s, 3H), 0.13 (s, 3H); MS (ESI) m/z 916.49, 918.47 (M+H).

A solution of compound S27-7-1 (14.2 mg, 0.0155 mmol) in 1,4-dioxane (1 mL) was treated with HF (0.40 mL, 48-50% aqueous solution). After stirring overnight, the mixture was poured into a solution of K₂HPO₄ (4.8 g) in water (20 mL). This mixture was extracted with EtOAc (3 times). The combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure. The material was dissolved in MeOH (1 mL), 1,4-dioxane (1 mL), and 0.5 N HCl/MeOH (0.2 mL). 100% Pd—C (Degussa, 5 mg) was added, and an atmosphere of hydrogen was introduced. After 5 hrs, the reaction mixture was filtered through Celite and concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Polymerx 10μ RP-γ100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: MeOH, gradient elution with 0→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product were concentrated to remove MeOH and were freeze-dried from 0.05N HCl in water/CH₃CN to yield 2.4 mg (25%) of compound S27-9-1: ¹H NMR (400 MHz, CD₃OD) δ 6.92 (d, J=6.4 Hz, 1H), 4.15 (s, 1H), 3.26-2.96 (m, 9H), 2.68-2.56 (m, 2H), 2.40-2.24 (m, 2H), 2.02-1.94 (m, 1H), 1.83 (s, 6H), 1.68-1.58 (m, 1H), 0.99 (dd, J=6.9, 3.7 Hz, 6H); MS (ESI) m/z 546.21 (M+H).

The following compounds were prepared according to the methods of preparing S27-9-1.

S27-9-2:

¹H NMR (400 MHz, CD₃OD) δ 6.96 (d, J=6.4 Hz, 1H), 4.16 (s, 1H), 3.26-2.96 (m, 9H), 2.61 (dd, J=18.4, 5.5 Hz, 2H), 2.40-2.26 (m, 2H), 1.87 (s, 6H), 1.70-1.58 (m, 1H), 1.01 (s, 9H); MS (ESI) m/z 560.26 (M+H).

Acetaldehyde (0.25 mL, 4.46 mmol) and sodium triacetoxyborohydride (60 mg, 0.28 mmol) were added to a solution of S27-9-1 (78.5 mg, 93.7 mmol) in acetic acid (0.5 mL) and CH₂Cl₂ (5 mL). After stirring overnight, additional acetaldehyde (0.25 mL, 4.46 mmol) and sodium triacetoxyborohydride (120 mg, 0.56 mmol) were added. After 3 h, the reaction mixture was diluted with pH 7.0 phosphate buffer and was extracted with CH₂Cl₂ (3 times). The combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure. The material was used directly in the next step: MS (ESI) m/866.78 (M+H).

A solution of compound S27-8-1 (81 mg, 0.094 mmol) in 1,4-dioxane (1 mL) was treated with HF (0.40 mL, 48-50% aqueous solution). After 30 min, the mixture was poured into a solution of K₂HPO₄ (4.8 g) in water (20 mL). This mixture was extracted with EtOAc (2 times). The combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure. The material was dissolved in MeOH (2 mL), 1,4-dioxane (2 mL), and 0.5 N HCl/MeOH (0.2 mL). 10% Pd—C (Degussa, 5 mg) was added, and an atmosphere of hydrogen was introduced. After 2 hrs, the reaction mixture was filtered through Celite and concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Polymerx 10μ RP-γ100 R column [30, 21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: MeOH, gradient elution with 20-100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product were concentrated to remove MeOH and were freeze-dried from 0.05N HCl in water/CH₃CN to yield 7.7 mg (13%) of compound S27-9-3: ¹H NMR (400 MHz, CD₃OD) δ 7.13 (d, J=2.8 Hz, 1H), 4.16 (s, 1H), 3.64-3.52 (m, 1H), 3.42-3.30 (m, 1H), 3.28-2.94 (m, 11H), 2.42-2.27 (m, 2H), 2.04-1.85 (m, 7H), 1.70-1.59 (m, 1H), 1.43 (dd, J=15.1, 7.3 Hz, 3H), 0.99 (dd, J=6.4, 5.9 Hz, 3H), 0.86 (dd, J=16.0, 6.0 Hz, 3H); MS (ESI) m/z 574.33 (M+H).

The following compounds were prepared according to the methods of preparing S27-9-3.

S27-9-4:

¹H NMR (400 MHz, CD₃OD) δ 7.12 (d, J=4.6 Hz, 1H), 4.16 (s, 1H), 3.26-2.88 (m, 16H), 2.42-2.24 (m, 2H), 1.92 (s, 3H), 1.88 (s, 1H), 1.70-1.58 (m, 1H), 1.00-0.94 (m, 9H); MS (ESI) m/z 574.32 (M+H).

S27-9-5:

¹H NMR (400 MHz, CD₃OD) δ 7.07 (d, J=4.6 Hz, 1H), 4.17 (s, 1H), 3.26-2.95 (m, 12H), 2.84-2.72 (m, 1H), 2.43-2.16 (m, 3H), 1.98-1.80 (m, 9H), 1.74-1.44 (m, 5H), 1.20-1.06 (m, 1H), 1.00-0.84 (m, 1H); MS (ESI) m/z 586.33 (M+H).

SX-9-6:

¹H NMR (400 MHz, CD₃OD) δ 7.13 (d, J=6.4 Hz, 1H), 4.17 (s, 1H), 3.64-3.52 (m, 1H), 3.45-3.32 (m, 1H), 3.30-2.95 (m, 9H), 2.43-2.26 (m, 2H), 2.26-2.10 (m, 1H), 1.98-1.76 (m, 9H), 1.74-1.54 (m, 6H), 1.50-1.40 (m, 3H), 1.28-1.16 (m, 1H), 1.00-0.84 (m, 1H); MS (ESI) m/z 600.40 (M+H).

S27-9-7:

¹H NMR (400 MHz, CD₃OD) δ 7.23 (s, 1H), 4.20 (s, 1H), 3.50-3.40 (m, 1H), 3.26-2.95 (m, 12H), 2.70-2.52 (m, 1H), 2.48-2.30 (m, 2H), 2.22-2.08 (m, 1H), 2.06-1.90 (m, 6H), 1.72-1.58 (m, 1H), 1.08-0.99 (m, 6H); MS (ESI) m/z 576.33 (M+H).

Example 28. Synthesis of Compounds Via Scheme 28

The following compounds were prepared according to Scheme 28.

Benzylbromide (0.687 mL, 5.78 mmol, 1.1 equiv) and K₂CO₃ powder (1.09 g, 7.88 mmol, 1.5 equiv) were added to a solution of compound S24-2 (1.772 g, 5.25 mmol, 1.0 equiv) in acetone (10 mL). The mixture was stirred at rt overnight. The reaction mixture was filter through celite pad to remove most of the solid. Solvents were evaporated and the residue was dissolved in a mixture of EtOAc (40 mL) and water (1:1). The organic layer was separated, and the aqueous layer was extracted with EtOAc (10 mL×2). The combined organic phase was dried over anhydrous magnesium sulfate, filtered, and concentrated. The residue was purified by flash column chromatography (5% to 20% EtOAc/hexanes) to afford the desired product S28-1 as white solid (2.213 g, 98%): ¹H NMR (400 MHz, CDCl₃) δ 7.49-7.33 (m, 7H), 7.26-7.22 (m, 1H), 7.15-7.04 (m, 3H), 5.10 (s, 2H), 3.79 (s, 3H), 2.42 (s, 3H); MS (ESI) m/z 449.13 (M+Na).

A solution of n-BuLi in hexanes (15.64 mL, 2.5 M, 39.1 mmol, 1.1 equiv) was added dropwise to a solution of compound S28-1 (15.18 g, 35.54 mmol, 1.0 equiv) in THF (100 mL) at −100° C. under a N₂ atmosphere. The resulting red solution was stirred at −100° C. for 5 min and then DMF (6.85 mL, 88.9 mmol, 2.5 equiv.) was added dropwise, slowly warmed to 0° C. in 1 hr. Saturated aqueous NH₄Cl was added. The resulting mixture was extracted three times with EtOAc (50 mL×3). The combined EtOAc extracts were washed with brine, dried (sodium sulfate), and concentrated. Purification of the residue by flash chromatography gave compound S28-2 (9.50 g, 71%) as white solid: ¹H NMR (400 MHz, CDCl₃) δ 10.38 (d, J=2.3 Hz, 1H), 7.46-7.41 (m, 2H), 7.41-7.31 (m, 5H), 7.29-7.24 (m, 2H), 7.11-7.06 (m, 2H), 5.17 (d, J=1.4 Hz, 2H), 3.88 (d, J=2.3 Hz, 3H), 2.42 (d, J=1.8 Hz, 3H); MS (ESI) m/z 399.22 (M+Na).

The compound S28-2 (595.6 mg, 1.583 mmol, 1.0 eq.) was dissolved in THF (8 mL), and (S)-(−)-2-methyl-2-propanesulfinamide (217.5 mg, 1.793 mmol, 1.1 eq.) was added. Ti(OEt)₄ (0.371 mL, 1.793 mmol, 1.1 eq.) was added slowly. After stirring overnight, the reaction mixture was poured into brine (10 mL) with stirring and was then filtered through a Celite pad. The resulting solution was extracted twice with ethyl acetate (10 mL). The combined organic extracts were washed with brine, dried over sodium sulfate, and concentrated to give the crude intermediate. Purification of the residue by flash chromatography gave compound S28-3 (647 mg, 85.2%) as an orange oil: ¹H NMR (400 MHz, CDCl₃) δ 8.90 (s, 1H), 7.47-7.40 (m, 3H), 7.39-7.28 (m, 4H), 7.27-7.21 (m, 1H), 7.18-7.13 (m, 1H), 7.12-7.07 (m, 2H), 5.09 (d, J=3.1 Hz, 2H), 3.78 (s, 3H), 2.40 (s, 3H), 1.24 (s, 9H); MS (ESI) m/z 480.33 (M+H).

Methylmagnesium bromide (3.0M solution in Et₂O, 0.367 mL, 1.10 mmol) was added to a −48° C. solution of S28-3 (264 mg, 0.550 mmol) in CH₂Cl₂ (3.5 mL). After 2 h, the solution was allowed to slowly warm to rt and stir overnight. The reaction was quenched with NH₄Cl (saturated, aqueous solution) and was extracted with EtOAc (1 time). The EtOAc extracts were washed with brine (1 time), dried over sodium sulfate, filtered and concentrated. The material was purified by column chromatography (Biotage 10 g column, 50 to 100% EtOAc in Hexanes gradient). This gave 179 mg (66%) of product S28-4 as a white solid: R_(f) 0.14 (70% EtOAc/hexanes); ¹H NMR (400 MHz, CDCl₃) δ 7.43-7.28 (m, 7H), 7.26-7.20 (m, 1H), 7.10-7.05 (m, 2H), 6.86 (s, 1H), 5.13-4.92 (m, 3H), 3.77 (s, 3H), 3.31 (d, J=2.8 Hz, 1H), 2.38 (s, 3H), 1.43 (d, J=6.9 Hz, 3H), 1.18 (s, 9H); MS (ESI) m/z 496.34 (M+H).

Compound S28-4 (179 mg, 0.361 mmol) was treated with 0.5M HCl in MeOH (5 mL). After 1 h, the reaction mixture was concentrated under reduced pressure. The material was used directly in the next step as the hydrochloride salt: MS (ESI) m/z 392.30 (M+H).

Sodium triacetoxyborohydride (76 mg, 0.36 mmol) was added to a solution of S28-5 (47 mg, 0.12 mmol) and formaldehyde (37% aqueous solution, 0.5 mL) in AcOH (0.5 mL) and CH₂Cl₂ (3 mL). After 30 min, the solution was diluted with EtOAc and was washed with NaHCO₃ (saturated, aqueous solution, 2 times) and brine (1 time). The organics were dried over sodium sulfate, filtered and concentrated. The material was purified by column chromatography (Biotage 10 g column, 0 to 10% MeOH in CH₂Cl₂ gradient). This gave 36.8 mg (73%) of product S28-6-1 as a thick oil: R_(f) 0.40 (10% MeOH/CH₂Cl₂); ¹H NMR (400 MHz, CDCl₃) δ 7.47-7.43 (m, 2H), 7.39-7.28 (m, 5H), 7.28-7.22 (m, 1H), 7.12-7.06 (m, 2H), 7.06 (s, 1H), 5.14 (dd, J=11.4, 2.8 Hz, 2H), 3.71 (s, 3H), 3.64-3.56 (m, 1H), 2.39 (s, 3H), 2.24 (s, 6H), 1.32 (d, J=6.4 Hz, 3H); MS (ESI) m/z 420.33 (M+H).

1,4-Dibromobutane (0.0285 mL, 0.240 mmol) was added to a solution of S28-5 (94 mg, 0.24 mmol) and triethylamine (0.134 mL, 0.960 mmol) in CH₃CN (3 mL), and the reaction mixture was heated to 130° C. (sealed). After 30 min, additional 1,4-dibromobutane (0.028 mL, 0.24 mmol) was added and heating was continued at 130° C. After 15 min, additional 1,4-dibromobutane (0.20 mL, 1.7 mmol) was added and heating was continued at 130° C. After 20 min, the reaction mixture was diluted with EtOAc and was washed with NaHCO₃ (saturated, aqueous solution, 2 times) and brine (1 time). The organics were dried over sodium sulfate, filtered and concentrated. The material was purified by column chromatography (Biotage 10 g column, 0 to 10% MeOH in CH₂Cl₂ gradient). This gave 48.7 mg (46%) of product S28-6-2 as a thick oil: ¹H NMR (400 MHz, CDCl₃) δ 7.48-7.39 (m, 2H), 7.37-7.26 (m, 5H), 7.26-7.18 (m, 1H), 7.12-7.04 (m, 3H), 5.13 (s, 2H), 3.38 (s, 3H), 3.66-3.56 (m, 1H), 2.57-2.47 (m, 2H), 2.44-2.34 (m, 5H), 1.80-1.68 (m, 4H), 1.38-1.30 (m, 3H); MS (ESI) m/z 446.73 (M+H).

n-Butyllithium (2.5 M/hexanes, 0.876 mL, 0.219 mmol) was added to diisopropylamine (0.310 mL, 0.219 mmol) in THF (3 mL) at −40° C. The reaction mixture was cooled to −78° C., and TMEDA (0.048 mL, 0.32 mmol) was added. A solution of compound S28-6-1 (36.8 mg, 0.0877 mmol) in THF (0.5 mL) was added dropwise. The reaction was stirred at −78° C. for 15 min. A solution of enone S1-9 (38.5 mg, 0.0800 mmol) in THF (0.5 mL) was added dropwise to the reaction mixture. The reaction was stirred from −78° C. to −20° C. for 1 h, quenched by saturated aqueous NH₄Cl, and extracted with EtOAc (1 time). The combined EtOAc extracts were washed with water (2 times) and brine (1 time), dried (sodium sulfate) and concentrated. The material was purified by column chromatography (Biotage 10 g column, 0 to 10% MeOH in CH₂Cl₂ gradient). This gave 31.4 mg (49%) of product S28-7-1 as a yellow solid: ¹H NMR (400 MHz, CDCl₃) δ 16.2 (s, 1H), 7.53-7.45 (m, 4H), 7.40-7.28 (m, 5H), 7.27-7.22 (m, 1H), 7.09 (s, 1H), 5.35 (s, 2H), 5.19 (q, J=12.8 Hz, 2H), 4.02 (d, J=10.4 Hz, 1H), 3.66 (s, 3H), 3.52-3.46 (m, 1H), 3.26 (dd, J=15.9, 4.9 Hz, 1H), 2.99-2.89 (m, 1H), 2.55-2.42 (m, 9H), 2.22-2.10 (m, 7H), 1.34-1.20 (m, 3H), 0.83 (s, 9H), 0.27 (s, 3H), 0.13 (s, 3H); MS (ESI) m/z 808.55 (M+H).

A solution of compound S28-7-1 (31.4 mg, 0.0389 mmol) in 1,4-dioxane (1 mL) was treated with HF (0.40 mL, 48-50% aqueous solution). After stirring overnight, the mixture was poured into a solution of K₂HPO₄ (4.8 g) in water (20 mL). This mixture was extracted with EtOAc (2 times). The combined extracts were dried over sodium sulfate, filtered, and concentrated under reduced pressure. The material was dissolved in MeOH and 0.5 M HCl in MeOH (2 mL) and was concentrated under reduced pressure. The material was dissolved in MeOH (2 mL) and 1,4-dioxane (2 mL). 10% Pd—C (Degussa, 5 mg) was added, and an atmosphere of hydrogen was introduced. After 1 h, the reaction mixture was filtered through Celite and concentrated under reduced pressure. The crude product was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Polymerx 10μ RP-γ100 R column [30×21.20 mm, 10 micron, solvent A: 0.05 N HCl, solvent B: CH₃CN, gradient elution with 0→100% B over 20 min; mass-directed fraction collection]. Fractions containing the desired product were freeze-dried to yield 18 mg (79%) of compound SX-6-1: ¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.67 (q, J=6.9 Hz, 1H), 4.13 (s, 1H), 3.80 (s, 3H), 3.24-2.94 (m, 12H), 2.75 (s, 3H), 2.44-2.34 (m, 1H), 2.33-2.24 (m, 1H), 1.76-1.59 (m, 4H); MS (ESI) m/z 516.13 (M+H).

Prepared from S28-7-2 according to the methods of compound S28-8-1: ¹H NMR (400 MHz, CD₃OD) δ 7.07 (s, 1H), 4.65 (q, J=6.9 Hz, 1H), 4.13 (s, 1H), 3.89-3.81 (m, 1H), 3.77 (s, 3H), 3.40-3.31 (m, 1H), 3.29-2.88 (m, 12H), 2.44-2.32 (m, 1H), 2.31-2.03 (m, 3H), 2.02-1.92 (m, 1H), 1.76-1.58 (m, 4H); MS (ESI) m/z 542.1 (M+H).

Example 29. Synthesis of Compounds Via Scheme 29

The following compounds were prepared according to Scheme 29.

To a solution of S2-4-14 (18 mg, 0.03 mmol) in concentrated H₂SO₄ (1 mL) was added sodium nitrate solution (0.07 mL of 0.5 M concentrated H₂SO₄ solution, 0.04 mmol) at 0° C. The mixture was stirred for 20 min and then transferred dropwise to a stirring diethyl ether solution (100 mL). The yellow precipitation was filtered with celite and washed with ether (30 mL). The precipitation was then flashed with methanol (30 mL) and concentrated.

To the residue in MeOH/dioxane solution (4:1, 5 mL) was added palladium on carbon (8 mg, 10 wt %) and HCl in MeOH (0.5 N, 0.1 mL). The reaction was stirred under H₂ (balloon) at 25° C. for 60 min. The mixture was filtered through a small Celite plug and flashed with MeOH. The filtrate was concentrated to yield the crude product. Preparative reverse phase HPLC purification on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-1 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 2.9 mL (0.05 N HCl/water); gradient: 0→100% B over 25 min; mass-directed fraction collection]. Fractions with the desired MW, eluting at 13.6-15.5 min, were collected and freeze-dried to yield 10.0 mg of S29-2-1: ¹H NMR (400 MHz, CD₃OD) δ 4.30 (s, 2H), 4.07 (s, 1H), 3.04 (s, 3H), 2.97 (s, 3H), 3.15-2.94 (comp, 5H), 2.28-2.03 (comp, 3H), 1.68-1.57 (m, 1H), 1.05 (d, J=6.7 Hz, 6H); MS (ESI) m/z 533.29 (M+H).

S29-2-2 was prepared from S2-4-28 according to the procedure for preparation of S29-2-1: ¹H NMR (400 MHz, CD₃OD) δ 4.68-4.60 (m, 1H), 4.20-4.12 (m, 1H), 4.07 (s, 1H), 3.03 (s, 3H), 2.96 (s, 3H), 3.13-2.93 (comp, 3H), 2.76 (s, 3H), 2.28-2.16 (m, 2H), 2.00-1.86 (m, 2H), 1.68-1.55 (m, 1H), 1.49 (s, 3H), 1.47 (s, 3H), 1.07 (t, J=7.3 Hz, 3H); MS (ESI) m/z 561.36 (M+H).

S29-2-3 was prepared from S2-4-50 according to the procedure for preparation of S29-2-1: ¹H NMR (400 MHz, CD₃OD) δ 4.40 (s, 2H), 4.08 (s, 1H), 3.49-3.20 (m, 4H), 3.04 (s, 3H), 2.96 (s, 3H), 3.14-2.94 (comp, 3H), 2.27-2.17 (comp, 2H), 1.78-1.56 (comp, 5H), 1.11 (s, 3H), 1.04 (s, 3H); MS (ESI) m/z 573.35 (M+H).

S29-2-4 was prepared from S1-14-61 according to the procedure for preparation of S29-2-1: ¹H NMR (400 MHz, CD₃OD) δ 4.61 (d, J=14.0 Hz, 1H), 4.57 (d, J=14.0 Hz, 1H), 4.09 (s, 1H), 3.55-3.36 (m, 4H), 3.03 (s, 3H), 2.95 (s, 3H), 3.13-2.94 (comp, 3H), 2.35-2.26 (m, 1H), 2.26-2.17 (m, 1H), 2.08-1.58 (comp, 9H); MS (ESI) m/z 575.30 (M+H).

Example 30. Synthesis of Compounds Via Scheme 30

The following compounds were prepared according to Scheme 30.

To a solution of aldehyde S10-5 (1.0 g, 2.09 mmol) in DMF (10 mL) was added NaH (167 mg, 4.18 mmol) and methyl iodide (390 μL, 6.27 mmol). The mixture was stirred at room temperature for 1 h and then quenched with water (2 mL). The solution was diluted with EtOAc (150 mL), washed with brine (50 mL×4), dried (Na₂SO₄), and concentrated. The residue was purified by flash chromatography on silica gel, eluting with hexanes/EtOAc (1:0 to 5:1) to afford imidazole S30-1 (310 mg, 30% yield); ¹H NMR (400 MHz, CDCl₃) δ 10.30 (s, 1H), 7.40-7.24 (comp, 8H), 7.04-6.99 (comp, 2H), 5.00-4.80 (m, 2H), 3.19 (s, 1.9H), 3.15 (s, 1.1H), 2.40 (d, J=2.4 Hz, 1.9H), 2.38 (d, J=2.4 Hz, 1.1H), 1.50 (s, 3.3H), 1.35 (s, 5.7H); MS (ESI) m/z 516.44 (M+Na).

To a solution of aldehyde S30-1 (200 mg, 0.41 mmol) in 1,2-dichloroethane (3 mL) was added isobutylamine (119 μL, 1.23 mmol) and acetic acid (92 μL, 1.64 mmol). The mixture was stirred at room temperature for 4 h. NaBH(OAc)₃ (254 mg, 1.20 mmol) was added. The reaction was stirred at room temperature for 15 h. The mixture was diluted with EtOAc (20 mL), washed with brine (5 mL×3), dried (Na₂SO₄) and concentrated. The residue was purified by flash chromatography on silica gel, eluting with hexanes/EtOAc (1:1) to give amine (126 mg, 56% yield); MS (ESI) m/z 551.47 (M+H).

To the amine (126 mg, 0.23 mmol) in DCM (4 mL) was added Boc₂O (150 mg, 0.69 mmol) and Et₃N (96 μL, 0.69 mmol). The reaction was stirred at room temperature for 4 h and then concentrated. The residue was purified by flash chromatography on silica gel, eluting with hexanes/EtOAc (7:1) to afford 148 mg of S30-2-1; MS (ESI) m/z 673.68 (M+Na).

To a solution of i-Pr₂NH (88 μL, 0.63 mmol) in THF (2 mL) was added a solution of n-BuLi (0.38 mL of a 1.70 M solution in hexanes, 0.63 mmol) dropwise at −78° C. The reaction was allowed to warm to 0° C. by removal of cooling bath, and then cooled to −78° C. TMEDA (93 μL, 0.63 mmol) was added, and the mixture was stirred at −78° C. for 5 min. A solution of amine S30-2-1 (148 mg, 0.23 mmol) in THF (0.5 mL) was added dropwise to the LDA solution over 5 min. Once addition was complete, the reaction mixture was stirred at −78° C. for 20 min. A solution of enone (100 mg, 0.21 mmol) in THF (0.5 mL) was added dropwise over 5 min. The mixture was slowly warmed to −20° C. over 45 min. The mixture was quenched with saturated ammonium chloride solution (5 mL), diluted with EtOAc (50 mL), washed with brine (10 mL×3), dried (Na₂SO₄), and concentrated. The residue was purified by flash chromatography on silica gel, eluting with hexanes/EtOAc (1:0 to 10:1) to afford 60 mg of compound S30-3-1 (28%): MS (ESI) m/z 1040.00 (M+H).

To a solution of S30-3-1 (60 mg, 0.06 mmol) in 1,4-dioxane (2 mL) was added hydrochloric acid (2 mL of 4 M solution) at room temperature. The mixture was stirred for 8 h. The mixture was diluted with EtOAc (20 mL), washed two times with potassium phosphate dibasic solution (prepared from 2 g K₂HPO₄ and 5 mL water), dried (Na₂SO₄) and concentrated to give a crude methyl aniline.

To a solution of methyl aniline in 1,4-dioxane (3 mL) was added HF (0.3 mL of 48% solution in water). The mixture was stirred at room temperature for 5 h. The mixture was quenched with potassium phosphate dibasic solution (prepared from 2 g K₂HPO₄ and 5 mL water). The aqueous layer was extracted with EtOAc (10 mL×3). All organic layers were combined, dried (Na₂SO₄) and concentrated. The residue was dissolved in ethyl acetate (5 mL), and hydrochloric acid (1 mL, 0.5 N in methanol) was added. The mixture was concentrated to give the HCl complex.

To the HCl complex in MeOH/dioxane solution (4:1, 5 mL) was added palladium on carbon (50 mg, 10 wt %). The reaction was stirred under H₂ (balloon) for overnight. The mixture was filtered through a small Celite plug and flashed with MeOH. The filtrate was concentrated to yield the crude product. Preparative reverse phase HPLC purification on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-1 100A column [10 μm, 150×21.20 mm: flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 3.2 mL (0.05 N HCl/water); gradient: 0→100% B over 25 min; mass-directed fraction collection]. Fractions with the desired MW, eluting at 13.3-15.1 min, were collected and freeze-dried to yield 19.1 mg of S30-4-1: ¹H NMR (400 MHz, CD₃OD) δ 4.52 (s, 2H), 4.12 (s, 1H), 3.76-3.56 (m, 1H), 3.05 (s, 3H), 3.04 (s, 3H), 2.97 (s, 3H), 3.24-2.94 (comp, 4H), 2.46-2.37 (m, 1H), 2.32-2.24 (m, 1H), 2.20-2.09 (m, 1H), 1.73-1.63 (m, 1H), 1.09 (d, J=6.7 Hz, 6H); MS (ESI) m/z 547.34 (M+H).

S30-4-2 was prepared according to the procedure for preparation of S30-4-1: ¹H NMR (400 MHz, CD₃OD) δ 4.66 (s, 2H), 4.12 (s, 1H), 3.05 (s, 3H), 3.01 (s, 3H), 3.01 (s, 3H), 3.00 (s, 3H), 2.97 (s, 3H), 3.22-2.94 (comp, 3H), 2.44-2.10 (m, 2H), 1.72-1.60 (m, 1H); MS (ESI) m/z 519.30 (M+H).

Example 31. Synthesis of Compounds Via Scheme 31

The following compounds were prepared according to Scheme 31.

To a mixture of S15-5 (3.12 g, 9.71 mmol, 1.0 equiv) and NIS (2.40 g, 10.68 mmol, 1.1 equiv) was added MeCN (20 mL) and TFA (224 μL, 2.91 mmol, 0.3 equiv). The resulting reaction mixture was stirred at reflux overnight. More NIS (874 mg, 3.88 mmol, 0.4 equiv) and TFA (75 μL, 0.97 mmol, 0.1 equiv) were added. The resulting reaction mixture was stirred at reflux overnight, and cooled to rt. The reaction mixture was diluted with EtOAc (200 mL), washed with saturated NaHCO₃ solution (70 mL), and sodium thiosulfate solution (2M, 70 mL), and brine (50 mL). The resulting organic phase was dried over anhydrous sodium sulfate, filtered, and concentrated to afford an orange solid, which was recrystallized from EtOAc/MeOH to give the product as a white solid (2.74 g). The mother liquor was concentrated, and the residue was purified by flash-column chromatography (0-15% ethyl acetate-hexanes) to afford the desired product S31-1 as a white solid (1.03 g, total yield: 87%): ¹H NMR (400 MHz, CDCl₃) δ 7.46-7.42 (m, 2H), 7.31-7.26 (m, 1H), 7.24-7.22 (m, 2H), 7.18 (s, 1H), 3.89 (s, 3H), 2.64 (s, 3H); MS (ESI) m/z 444.93, 446.95 (M−H).

To compound S31-1 (447 mg, 1.00 mmol, 1.0 equiv), pyrrolidine (0.165 mL, 2.00 mmol, 2.0 equiv), cesium carbonate (489 mg, 1.5 mmol, 1.5 equiv), and Xantphos (31.8 mg, 0.055 equiv) in dry dioxane (5 mL) at room temperature was added Pd₂(dba)₃ (22.8 mg, 0.05 equiv Pd). The mixture was purged by bubbling with dry nitrogen gas for 5 min with gentle stirring. The reaction vessel was then heated under nitrogen at 100° C. for 5 hrs with rapid stirring. The resulting mixture was cooled to rt, diluted with water (20 mL), and extracted with EtOAc (10 mL×3). The EtOAc extracts were dried over sodium sulfate and concentrated under reduced pressure. Flash column chromatography on silica gel with 5%-30% EtOAc/hexanes afforded the desired product S31-2 (156 mg, 40%) as a pale colorless oil; MS (ESI) m/z 390.30 (M+H).

A solution of BBr₃ in dichloromethane (0.6 mL, 1.0 M, 0.6 mmol, 1.5 equiv) was added slowly to a solution of the above compound S31-2 in dichloromethane (5 mL) at −78° C. The resulting light yellow solution was allowed to warm to 0° C. in 30 min and kept at that temperature for 10 min (monitored by LC-MS). The reaction mixture was poured into saturated NaHCO₃ solution (10 mL), stirred at rt for 5 min, and extracted with dichloromethane (10 mL×2). The organic extracts were combined and dried over anhydrous magnesium sulfate, filtered, and concentrated to afford the crude phenol, which was used directly in the next reaction; MS (ESI) m/z 376.27 (M+H).

Benzylbromide (59.3 μL, 0.5 mmol, 1.25 equiv) and K₂CO₃ powder (110 mg, 0.8 mmol, 2 equiv) were added to a solution of the above crude phenol (0.4 mmol, 1.0 equiv) in acetonitrile (10 mL). The mixture was stirred at rt overnight. Solvents were evaporated and the residue was dissolved in a mixture of EtOAc (20 mL) and water (1:1). The organic layer was separated, and the aqueous layer was extracted with EtOAc (10 mL×2). The combined organic phase was dried over anhydrous magnesium sulfate, filtered, and concentrated. The residue was purified by flash column chromatography (5% to 25% EtOAc/hexanes) to afford the desired product S31-3 as a colorless oil (151 mg, 81% two steps): ¹H NMR (400 MHz, CDCl₃) δ 7.44-7.35 (m, 7H), 7.26-7.22 (m, 1H), 7.09-7.07 (m, 3H), 5.10 (s, 2H), 3.50-3.41 (m, 4H), 2.34 (d, J=2.4 Hz, 3H), 2.00-1.90 (m, 4H); MS (ESI) m/z 466.34 (M+H).

A solution of n-butyllithium in hexanes (0.30 mL, 1.6 M, 0.48 mmol, 1.5 equiv) was added to a solution of diisopropylamine (67.3 μL, 0.48 mmol, 1.5 equiv) and TMEDA (71.9 μL, 0.48 mmol, 1.5 equiv) in THF (2 mL) at −78° C. The reaction solution was warmed to −20° C. and then re-cooled to −78° C. A solution of compound S31-3 (149 mg, 0.32 mmol, 1.0 equiv) in THF (1.5 mL) was added dropwise via a cannula. The resulting red reaction mixture was then stirred at −78° C. for 10 min, and cooled to −100° C. A solution of enone S11-9 (154 mg, 0.32 mmol, 1.0 equiv) in THF (1 mL) was added to the reaction mixture via a cannula, followed by LHMDS solution (0.4 mL, 1.0 M, 1.2 eq.). The resulting reaction mixture was allowed to warm to −30° C. over 1.5 hrs, quenched by saturated aqueous NH₄Cl (10 mL), and extracted with EtOAc (10 mL×3). The organic phase was dried over sodium sulfate, filtered and concentrated. The residue was purified by a preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 3.0 mL (CH₃CN); gradient: 80→100% B in A over 10 min; mass-directed fraction collection]. Fractions containing the desired product were collected and concentrated to yield compound S31-4 (40.1 mg, 14.7%): ¹H NMR (400 MHz, CDCl₃) δ 16.00 (br s, 1H), 7.50-7.46 (m, 4H), 7.39-7.27 (m, 6H), 7.10 (s, 1H), 5.36 (s, 2H), 5.18, 5.12 (ABq, J=12.8 Hz, 2H), 4.10 (d, J=10.4 Hz, 1H), 3.75-3.65 (m, 2H), 3.59-3.50 (m, 2H), 3.37 (dd, J=4.3, 15.9 Hz, 1H), 2.88-2.74 (m, 1H), 2.55-2.40 (m, 9H), 2.12 (d, J=14.0 Hz, 1H), 1.99-1.83 (m, 4H), 0.84 (s, 9H), 0.28 (s, 3H), 0.14 (s, 3H); MS (ESI) m/z 854.72 (M+H).

A solution of phenyllithium in di-n-butyl ether (39 μL, 1.8 M, 0.07 mmol, 1.5 equiv) was added dropwise to a solution of compound S31-4 (40 mg, 0.047 mmol, 1.0 equiv) in THF (1.5 mL) at −78° C., forming an orange solution. After 5 min, a solution of n-butyllithium in hexanes (35.3 μL, 1.6 M, 0.056 mmol, 1.2 equiv) was added dropwise at −78° C., followed 2 min later by the addition of N,N-dimethylformamide (18.1 μL, 5.0 equiv). The resulting dark red reaction mixture was stirred at −78° C. for 1 hour. The reaction mixture was allowed to warm to −40° C. LCMS indicate only about 30% product present in reaction mixture. Saturated aqueous ammonium chloride (5 mL) was added dropwise at −40° C. The reaction mixture was allowed to warm to 23° C., diluted with saturated aqueous ammonium chloride (˜10 mL), and extracted with EtOAc (2×15 mL). The organic extracts were combined, dried over anhydrous sodium sulfate, filtered, and concentrated, affording compound S31-5 as orange oil, which was used directly in the next reactions: MS (ESI) m/z 804.73 (M+H).

(R)-2,3,3-trimethyl-butylamine (3.8 μL, 0.03 mmol, 2.0 equiv), acetic acid (4.5 μL, 0.045 mmol, 3.0 equiv) and sodium triacetoxyborohydride (6.4 mg, 0.03 mmol, 2.0 equiv) were added sequentially to a solution of compound S31-5 (crude product, 0.014 mmol, 1.0 equiv) in 1,2-dichloroethane (2 mL) at 23° C. After stirring for overnight, the reaction mixture was quenched by the addition of saturated aqueous sodium bicarbonate (5 mL) and extracted with dichloromethane (2×10 mL). The combined organic extracts were dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Sunfire Prep C18 OBD column [5 μm, 19×50 mm; flow rate, 20 mL/min; Solvent A: H₂O with 0.1% HCO₂H; Solvent B: CH₃CN with 0.1% HCO₂H; injection volume: 3.0 mL (CH₃CN); gradient: 20→100% B in A over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 5.6-6.5 min, were collected and freeze-dried to yield compound S31-6-1 (7.5 mg, 18% for 2 steps): MS (ESI m/z 889.83 (M+H).

Aqueous HF (48-50%, 0.2 mL) was added to a solution of compound S31-6-1 (7.5 mg, 0.0084 mmol, 1.0 equiv) in acetonitrile (1.0 mL) in a polypropylene reaction vessel at 23° C. The mixture was stirred vigorously at 23° C. overnight and poured into aqueous K₂HPO₄ (2.0 g dissolved in 10 mL water). The mixture was extracted with EtOAc (3×10 mL). The combined organic extracts were dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was used directly in the next step without further purification.

Pd—C (10 wt %, 5 mg) was added in one portion into the yellow solution of the above crude product in a mixture of HCl/MeOH (0.5 N, 0.25 mL) and MeOH (2 mL) at 23° C. The reaction vessel was sealed and purged with hydrogen by briefly evacuating the flask followed by flushing with hydrogen gas (1 atm). The resulting mixture was stirred at 23° C. for 60 min. The reaction mixture was then filtered through a small Celite pad. The filtrate was concentrated. The residue was purified by preparative reverse phase HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100A column [10 μm, 150×21.20 mm; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: CH₃CN; injection volume: 3.0 mL (0.05 N HCl/water); gradient: 15→60% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product, eluting at 5.8-8.0 min, were collected and freeze-dried to yield compound S31-7-1 (2.77 mg, 54% for 2 steps): ¹H NMR (400 MHz, CD₃OD) δ 7.01 (s, 1H), 4.36 (d, J=12.8 Hz, 1H), 4.14 (d, J=12.8 Hz, 1H), 4.12 (s, 1H), 3.56-3.49 (m, 4H), 3.13-2.84 (m, 10H), 2.39-2.12 (m, 2H), 2.03-1.91 (m, 4H), 1.70-1.60 (m, 1H), 1.47 (s, 9H), 1.35 (d, J=6.9 Hz, 3H), 1.00 (s, 9H); MS (ESI) m/z 597.25 (M+H).

Example 32. Synthesis of Compounds Via Scheme 32

The following compounds were prepared according to Scheme 32.

Compound S16-4a (1.61 g, 3.57 mmol, 1.0 equiv) was dissolved in 1,2-dichloroethane (20 mL). Acetaldehyde (1.2 mL, 21.42 mmol, 6.0 equiv) and acetic acid (0.62 mL, 10.71 mmol, 3.0 equiv) were added. After stirring at rt for 1 h, sodium triacetoxyborohydride (2.27 g, 10.71 mmol, 3.0 equiv) was added. Stirring was continued for 1 hr. The reaction mixture was poured into saturated aqueous NaHCO₃ and extracted three times with dichloromethane. The combined organic extracts were washed with brine, dried over sodium sulfate, and concentrated to give the crude intermediate. Purification of the residue by flash chromatography gave compound S32-1-1 (1.52 g, 91%) as a white solid: ¹H NMR (400 MHz, CDCl₃) δ 7.45-7.40 (m, 2H), 7.39-7.30 (m, 5H), 7.25-7.20 (m, 1H), 7.09 (s, 1H), 7.07-7.03 (m, 2H), 5.08 (s, 2H), 3.23-3.05 (m, 4H), 2.42 (s, 3H), 1.00 (t, J=7.1 Hz, 6H); MS (ESI) m/z 468.39 (M+H).

Compound S16-4a (1.61 g, 3.57 mmol, 1.0 equiv) was dissolved in 1,2-dichloroethane (20 mL). Acetone (0.786 mL, 10.71 mmol, 3.0 equiv) and acetic acid (0.62 mL, 10.71 mmol, 3.0 equiv) were added. After stirring at rt for 1 h, sodium triacetoxyborohydride (1.14 g, 5.35 mmol, 1.5 equiv) was added. Stirring was continued for overnight. The reaction mixture was poured into saturated aqueous NaHCO₃ and extracted three times with dichloromethane. The combined organic extracts were washed with brine, dried over sodium sulfate, and concentrated to give the crude intermediate. Purification of the residue by flash chromatography gave compound (970 mg, 2.14 mmol, 60%) as a white solid: MS (ESI) m/z 454.36 (M+H).

The compound was redissolved in 1,2-dichloroethane (20 mL), followed by acetic acid (0.367 mL, 6.41 mmol, 3.0 equiv) and formaldehyde (0.478 mL, 37%, 6.41 mmol, 3.0 eq.). After stirring at rt for 1 h, sodium triacetoxyborohydride (0.907 mg, 4.28 mmol, 2.0 equiv) was added. Stirring was continued for overnight. The reaction mixture was poured into saturated aqueous NaHCO₃ and extracted three times with dichloromethane. The combined organic extracts were washed with brine, dried over sodium sulfate, and concentrated to give the crude intermediate. Purification of the residue by flash chromatography gave compound S32-1-2 (1.08 g, 66% for two steps) as a white solid: ¹H NMR (400 MHz, CDCl₃) δ 7.45-7.31 (m, 7H), 7.27-7.22 (m, 1H), 7.09 (s, 1H), 7.08-7.04 (m, 2H), 5.09 (s, 2H), 3.60-3.50 (m, 1H), 2.76 (s, 3H), 2.41 (s, 3H), 1.15 (d, J=6.4 Hz, 3H), 0.94 (d, J=6.4 Hz, 3H); MS (ESI) m/z 468.36 (M+H).

A solution of n-BuLi in hexanes (1.05 mL, 1.6 M, 1.68 mmol, 1.4 equiv) was added dropwise to a solution of i-Pr₂NH (0.235 mL, 1.68 mmol, 1.4 equiv) in THF (5 mL) at −78° C. under a N₂ atmosphere. The resulting solution was stirred at −78° C. for 20 min and −20° C. for 5 min, and then re-cooled to −78° C. N,N,N′,N′-Tetramethylethylenediamine (TMEDA, 0.252 mL, 1.68 mmol, 1.4 equiv) was added, followed by dropwise addition of compound S32-1-1 (620 mg, 1.32 mmol, 1.1 equiv) in THF (3 mL) via syringe. After complete addition, the resulting dark-red mixture was stirred for another 15 min at −78° C. A solution of enone S1-9 (580 mg, 1.20 mmol, 1.0 equiv) in THF (2 mL) was added dropwise via syringe. LHMDS (1.44 mL, 1.0 M/THF, 1.44 mmol, 1.2 equiv) was then added and the reaction was slowly warmed to −20° C. Saturated aqueous NH₄Cl was added. The resulting mixture was extracted three times with EtOAc. The combined EtOAc extracts were washed with brine, dried (sodium sulfate), and concentrated. Purification of the residue by flash chromatography gave compound S32-2-1 (627 mg, 62%) as light yellow foam: ¹H NMR (400 MHz, CDCl₃) δ 16.0 (s, 1H), 7.52-7.45 (m, 4H), 7.42-7.27 (m, 6H), 7.12 (s, 1H), 5.35 (s, 2H), 5.22-5.09 (m, 2H), 4.00 (d, J=9.8 Hz, 1H), 3.62-3.52 (m, 1H), 3.30-3.09 (m, 3H), 3.04-2.93 (m, 1H), 2.87-2.75 (m, 1H), 2.58-2.30 (m, 9H), 2.10 (d, J=14.0 Hz, 1H), 1.03 (t, J=7.1 Hz, 3H), 0.91 (t, J=7.1 Hz, 3H), 0.82 (s, 9H), 0.26 (s, 3H), 0.13 (s, 3H); MS (ESI) m/z 856.50 (M+H).

A solution of phenyllithium in di-n-butyl ether (0.345 mL, 1.8 M, 0.620 mmol, 1.5 equiv) was added dropwise to a solution of compound S32-2-1 (352 mg, 0.414 mmol, 1.0 equiv) in THF (10 mL) at −78° C., forming an orange solution. After 5 min, a solution of n-butyllithium in hexanes (0.311 mL, 1.6 M, 0.497 mmol, 1.2 equiv) was added dropwise at −78° C., followed 2 min later by the addition of N,N-dimethylformamide (0.160 mL, 2.07 mmol, 5.0 equiv). The resulting dark red reaction mixture was stirred at −78° C. for 10 min then warm up to −40° C. in 30 min. Saturated aqueous ammonium chloride (10 mL) was added dropwise at −78° C. The reaction mixture was allowed to warm to 23° C., diluted with saturated aqueous ammonium chloride (˜20 mL), and extracted with EtOAc (2×25 mL). The organic extracts were combined, dried over anhydrous sodium sulfate, filtered, and concentrated, affording compound S32-3-1 as an orange oil (419 mg, with reduced by-product), which was used directly in the next reactions: ¹H NMR (400 MHz, CDCl₃) δ 15.9 (s, 1H), 10.31 (s, 1H), 7.50-7.46 (m, 4H), 7.39-7.26 (m, 7H), 5.36 (s, 2H), 5.30-5.13 (m, 2H), 4.01 (d, J=11.0 Hz, 1H), 3.62-3.52 (m, 1H), 3.30-3.09 (m, 3H), 3.04-2.93 (m, 1H), 2.87-2.75 (m, 1H), 2.58-2.30 (m, 9H), 2.15 (d, J=14.0 Hz, 1H), 1.03 (t, J=7.1 Hz, 3H), 0.91 (t, J=7.1 Hz, 3H), 0.82 (s, 9H), 0.27 (s, 3H), 0.14 (s, 3H); MS (ESI) m/z 806.76

Compound S32-3-1 (100 mg, 0.05 mmol, 1.0 equiv) was dissolved in 1,2-dichloroethane (2.0 mL). Cyclohexylamine (17.2 μL, 0.15 mmol, 3.0 equiv) and acetic acid (11.45 μL, 0.20 mmol, 4.0 equiv) were added. After stirring at rt for 1 h, sodium triacetoxyborohydride (31.8 mg, 0.15 mmol, 3.0 equiv) was added. Stirring was continued overnight. The reaction mixture was poured into saturated aqueous NaHCO₃ and extracted three times with dichloromethane. The combined organic extracts were washed with brine, dried over sodium sulfate, and concentrated to give the crude intermediate S32-4-1-1 (27.5 mg), which was used directly in the next step without further purification: MS (ESI) m/z 889.85 (M+H).

In a plastic vial, the above amine intermediate was dissolved in CH₃CN (1 mL). Aqueous HF (48-50%, 0.25 mL) was added. After stirring at rt for 16 hrs, the reaction mixture was poured into aqueous solution (10 mL) of K₂HPO₄ (2.0 g) and extracted three times with dichloromethane. The combined organic phases were washed with brine, dried, and concentrated to yield the crude intermediate: MS (ESI) m/z 775.72 (M+H).

The above crude intermediate was dissolved in MeOH (2 mL) with 0.5 N HCl/MeOH (0.20 mL). Pd—C (10 wt %, 5 mg) was added. The reaction flask was briefly evacuated and re-filled with hydrogen. The reaction mixture was stirred at rt and monitored by LC-MS. After the reaction was complete, the mixture was filtered through a small pad of Celite. The filtrate was concentrated to give the crude product, which was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 20→100% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product were collected and freeze-dried to give the desired product as a yellow solid S32-6-1-1 (4.35 mg, 7.4% for four steps): ¹H NMR (400 MHz, CD₃OD) δ 7.00 (s, 1H), 4.49, 4.32 (ABq, J=14.7 Hz, 2H), 4.08 (s, 1H), 3.22-2.90 (m, 13H), 2.39-2.28 (m, 1H), 2.27-2.14 (m, 3H), 1.97-1.86 (m, 2H), 1.79-1.70 (m, 1H), 1.69-1.57 (m, 1H), 1.50-1.35 (m, 4H), 1.35-1.19 (m, 2H), 1.08 (t, J=6.9 Hz, 3H), 0.99 (t, J=6.9 Hz, 3H); MS (ESI) m/z 597.30 (M+H).

The following compounds were prepared according to the methods for S32-6-1-1, substituting the appropriate amine for cyclohexylamine.

S32-6-1-2:

¹H NMR (400 MHz, CD₃OD) δ 6.98 (s, 1H), 4.48, 4.32 (ABq, J=15.7 Hz, 2H), 4.06 (s, 1H), 3.18-2.89 (m, 15H), 2.40-2.28 (m, 1H), 2.24-2.14 (m, 1H), 2.15-2.05 (m, 1H), 1.70-1.58 (m, 1H), 1.07 (d, J=6.4 Hz, 6H), 0.99 (t, J=6.9 Hz, 6H); MS (ESI) m/z 571.29 (M+H).

S32-6-1-3:

¹H NMR (400 MHz, CD₃OD) δ 7.05 (s, 1H), 4.49 (s, 2H), 4.09 (s, 1H), 3.17-2.93 (m, 14H), 2.40-2.30 (m, 1H), 2.23-2.17 (m, 1H), 1.70-1.57 (m, 1H), 1.38 (d, J=6.4 Hz, 3H), 1.13 (t, J=6.9 Hz, 3H), 1.08 (s, 9H), 0.99 (t, J=6.9 Hz, 3H); MS (ESI) m/z 599.39 (M+H).

S32-6-1-4:

¹H NMR (400 MHz, CD₃OD) δ 7.05 (s, 1H), 4.50, 4.28 (ABq, J=14.7 Hz, 2H), 4.08 (s, 1H), 3.17-2.92 (m, 14H), 2.39-2.30 (m, 1H), 2.24-2.16 (m, 1H), 1.70-1.57 (m, 1H), 1.48 (s, 9H), 1.09 (t, J=6.9 Hz, 3H), 1.00 (t, J=6.9 Hz, 3H); MS (ESI) m/z 571.29 (M+H).

S32-6-1-5:

¹H NMR (400 MHz, CD₃OD) δ 7.04 (s, 1H), 4.68, 4.52 (ABq, J=14.7 Hz, 2H), 4.08 (s, 1H), 3.73-3.59 (m, 2H), 3.17-2.92 (m, 15H), 2.39-2.30 (m, 1H), 2.28-2.01 (m, 5H), 1.70-1.57 (m, 1H), 1.08 (t, J=6.9 Hz, 3H), 1.00 (t, J=6.9 Hz, 3H); MS (ESI) m/z 569.29 (M+H).

S32-6-1-6:

¹H NMR (400 MHz, CD₃OD) δ 7.10 (s, 0.5H), 7.07 (s, 0.5H), 4.65 (d, J=12.8 Hz, 0.5H), 4.58 (d, J=12.8 Hz, 0.5H), 4.43 (d, J=12.8 Hz, 0.5H), 4.30 (d, J=12.8 Hz, 0.5H), 4.10 (s, 1H), 3.24-2.81 (m, 15H), 2.43-2.33 (m, 1H), 2.27-1.99 (m, 5H), 1.71-1.58 (m, 1H), 1.64 (s, 3H), 1.45 (s, 3H), 1.12-1.00 (m, 6H); MS (ESI) m/z 597.30 (M+H).

S32-6-1-7:

¹H NMR (400 MHz, CD₃OD) δ 7.09 (s, 1H), 4.68, 4.47 (ABq, J=14.7 Hz, 2H), 4.08 (s, 1H), 3.57-3.40 (m, 2H), 3.24-2.90 (m, 15H), 2.41-2.31 (m, 1H), 2.23-1.97 (m, 5H), 2.07-1.84 (m, 4H), 1.83-1.70 (m, 4H), 1.69-1.58 (m, 1H), 1.08 (t, J=6.9 Hz, 3H), 1.02 (t, J=6.9 Hz, 3H); MS (ESI) m/z 597.37 (M+H).

Compound S32-6-2-1 to S32-6-2-3 was prepared similarly from S32-1-2: ¹H NMR (400 MHz, CD₃OD) δ 7.05 (s, 0.4H), 7.04 (s, 0.6H), 4.80-4.64 (m, 0.8H), 4.43-4.20 (m, 1.2H), 4.08 (s, 0.4H), 4.07 (s, 0.6H), 3.67-3.53 (m, 2H), 3.20-2.78 (m, 15H), 2.52-2.30 (m, 1H), 2.26-2.17 (m, 1H), 2.05-1.58 (m, 9H), 1.26 (d, J=6.4 Hz, 1.8H), 1.21 (d, J J=6.4 Hz, 1.2H), 0.94 (d, J=6.4 Hz, 1.8H), 0.79 (d, J=6.4 Hz, 1.2H); MS (ESI) m/z 597.35 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 7.06 (s, 0.4H), 7.03 (s, 0.6H), 4.70-4.64 (m, 0.8H), 4.40-4.20 (m, 1.2H), 4.08 (s, 0.6H), 4.07 (s, 0.4H), 3.18-2.79 (m, 14H), 2.49-2.30 (m, 1H), 2.26-2.17 (m, 1H), 1.71-1.58 (m, 1H), 1.41-1.20 (m, 6H), 1.08 (s, 4H), 1.05 (s, 5H), 0.98-0.79 (m, 3H); MS (ESI) m/z 599.38 (M+H).

¹H NMR (400 MHz, CD₃OD) δ 6.98 (s, 0.5H), 6.97 (s, 0.5H), 4.58 (d, J=12.8 Hz, 0.5H), 4.45 (d, J=12.8 Hz, 0.5H), 4.31 (d, J=12.8 Hz, 0.5H), 4.15 (d, J=12.8 Hz, 0.5H), 4.09 (s, 0.5H), 4.08 (s, 0.5H), 3.17-2.90 (m, 11H), 2.86 (s, 1.5H), 2.75 (s, 1.5H), 2.46-2.28 (m, 1H), 2.27-2.13 (m, 3H), 1.97-1.85 (m, 2H), 1.70-1.58 (m, 1H), 1.50-1.34 (m, 5H), 1.33-1.16 (m, 4H), 0.95 (d, J=6.4 Hz, 1.5H), 0.81 (d, J=6.4 Hz, 1.5H), MS (ESI) m/z 597.37 (M+H).

Example 33. Synthesis of Compounds Via Scheme 33

The following compounds were prepared according to Scheme 33.

Compound S15-8 (2.51 g, 6.59 mmol) was suspended in methanol (25 mL) and sodium borohydride (373 mg, 9.88 mmol) was added in several portions. After gas evolution ceased and complete solution was achieved, the reaction mixture was quenched with NaHCO₃ (saturated, aqueous solution) and was extracted with EtOAc (3×). The organics were dried over Na₂SO₄, filtered, and concentrated under reduced pressure. This gave 2.49 g (99%) of S33-1 as a white solid. ¹H NMR (400 MHz, CDCl₃) δ 7.46-7.32 (m, 7H), 7.27-7.21 (m, 1H), 7.13 (s, 1H), 7.07 (d, J=8.7 Hz, 2H), 5.16 (s, 2H), 4.77 (d, J=6.4 Hz, 2H), 2.46 (s, 3H), 2.06 (t, J=6.4 Hz, 1H); MS (ESI) m/z 405.15 (M+H).

10% Palladium on carbon (Degussa, 50 mg) was added to a solution of compound S33-2 (1.85 g, 4.84 mmol) in EtOAc (10 mL), Methanol (10 mL), and chlorobenzene (1.5 mL) and an atmosphere of hydrogen was introduced. After 5 hours, the reaction mixture was purged with nitrogen and was filtered through Celite. The filtrate was concentrated under reduced pressure, yielding the phenol intermediate as a white solid. The intermediate was dissolved in acetic acid (15 mL) and sodium acetate (0.595 g, 7.26 mmol) was added. Bromine (0.372 mL, 7.26 mmol) was added dropwise over ˜3 min. After 10 min, the reaction mixture was quenched with Na₂S₂O₃ (5% aqueous solution) and was diluted with EtOAc. The layers were separated, and the EtOAc layer was washed with water (3×) and brine (1×). The organics were dried over Na₂SO₄, filtered, and concentrated under reduced pressure. The material was dissolved in acetone (30 mL), and K₂CO₃ (1.34 g, 9.68 mmol) and benzylbromide (0.633 mL, 5.32 mmol) were added. The reaction mixture was heated to 50° C. overnight. Upon cooling to rt, the reaction mixture was diluted with EtOAc and was washed with water (3×) and brine (1×). The organics were dried over Na₂SO₄, filtered, and concentrated under reduced pressure. The material was purified by column chromatography (Biotage 50 g column, 7 to 60% EtOAc in hexane gradient), yielding 2.03 g (91%) of S33-2. ¹H NMR (400 MHz, CDCl₃) δ 7.51-7.47 (m, 2H), 7.41-7.31 (m, 5H), 7.30-7.23 (m, 1H), 7.03 (d, J=8.2 Hz, 2H), 5.12-5.05 (m, 4H), 2.48 (s, 3H), 2.18 (t, J=7.1 Hz, 1H); MS (ESI) m/z 482.99, 484.99, 486.99 (M+Na).

Compound S33-2 (195 mg, 0.423 mmol) was dissolved in dichloromethane (4 mL) and DMSO (60.1 μL, 0.846 mmol, 2.0 eq.) was added dropwise at −30° C. After stirred for 5 minutes, trifluoroacetic anhydride (TFAA, 0.117 mL, 0.846 mmol, 2.0 eq.) was added slowly in 5 min. The reaction mixture was warmed up to −20° C. and stirred another 15 min. Triethylamine (0.173 mL, 1.27 mmol, 3.0 eq.) was added and reaction mixture was allow to warm up to rt. The reaction mixture was quenched with NH₄Cl (saturated, aqueous solution) and was extracted with EtOAc (3×10 mL). The organics were dried over Na₂SO₄, filtered, and concentrated under reduced pressure. The material was purified by column chromatography (Biotage 10 g column, 5 to 20% EtOAc in hexane gradient), yielding 150 mg (76%) of 533-3 and recover 37 mg compound S-33-2. ¹H NMR (400 MHz, CDCl₃) δ 10.34 (s, 1H), 7.51-7.45 (m, 2H), 7.43-7.31 (m, 5H), 7.31-7.23 (m, 1H), 7.03 (d, J=8.2 Hz, 2H), 5.13 (s, 2H), 2.49 (s, 3H); MS (ESI) m/z 481.02, 483.02 (M+Na).

To a solution of aldehyde S33-3 (156 mg, 0.34 mmol, 1.0 equiv) in MeOH (4 mL) was added trimethylorthoformate (231 μL, 2.1 mmol, 5.0 equiv) and TsOH (8 mg, 0.1 equiv). The reaction was heated to 65° C. for overnight. The solvent was evaporated. The residue was redissolved in EtOAc (20 mL), washed with saturated aqueous NaHCO₃ and brine, dried over sodium sulfate, and concentrated. Purification of the residue by flash chromatography gave compound S33-4 (163 mg, 94.5%) as a white solid: ¹H NMR (400 MHz, CDCl₃) δ 7.44-7.40 (m, 2H), 7.38-7.30 (m, 5H), 7.25-7.20 (m, 1H), 7.09-7.03 (m, 2H), 5.60 (s, 1H), 5.16 (s, 2H), 3.35 (s, 6H), 2.46 (s, 3H); MS (ESI) m/z 527.09 (M+Na).

To a solution of compound S33-4 (163 mg, 0.32 mmol, 1.0 equiv) in anhydrous THF (3 mL) was added i-PrMgCl.LiCl (0.536 mL, 1.2 M/THF, 0.643 mmol, 2.0 equiv) dropwise at −78° C. under a N₂ atmosphere. After 10 min, the temperature was raised to 0° C. and the reaction was stirred for 1 h at 0° C. The reaction mixture was cooled to −60° C. and N-fluorobenzenesulfonimide (304 mg, 0.964 mmol, 3.0 eq.) in 1 mL THF solution was added slowly. The reaction was warmed to −20° C., stirred for 30 min at rt, and quenched by saturated aqueous NH₄Cl. The layers were separated and the aqueous layer was further extracted twice with EtOAc. The combined organic layers were washed with brine, dried over sodium sulfate, filtered, and concentrated. Purification of the residue by flash chromatography gave compound S33-5 (93 mg, 65%) as a white solid: ¹H NMR (400 MHz, CDCl₃) δ 7.46-7.42 (m, 2H), 7.40-7.31 (m, 5H), 7.26-7.22 (m, 1H), 7.09-7.03 (m, 2H), 5.62 (s, 1H), 5.19 (s, 2H), 3.36 (s, 6H), 2.48 (s, 3H); MS (ESI) m/z 467.15 (M+Na).

A solution of n-BuLi in hexanes (196 μL, 1.6 M, 0.313 mmol, 1.5 equiv) was added dropwise to a solution of i-Pr₂NH (43.9 μL, 0.313 mmol, 1.5 equiv) in THF (2 mL) at −78° C. under a N₂ atmosphere. The resulting solution was stirred at −78° C. for 20 min and −20° C. for 5 min, and then re-cooled to −78° C. N,N,N′,N′-Tetramethylethylenediamine (TMEDA, 62.7 μL, 0.418 mmol, 2.0 equiv) was added, followed by dropwise addition of S33-5 (93 mg, 0.209 mmol, 1.0 equiv) in THF (1 mL) via syringe. After complete addition, the resulting dark-red mixture was stirred for another hour at −78° C. and then cooled to −100° C. A solution of enone S1-9 (100.9 mg, 0.209 mmol, 1.0 equiv) in THF (1 mL) was added dropwise via syringe. The resulting red mixture was slowly warmed to −78° C. LHMDS (0.313 mL, 1.0 M/THF, 0.313 mmol, 1.5 equiv) was then added and the reaction was slowly warmed to −20° C. Saturated aqueous NH₄Cl was added. The resulting mixture was extracted three times with EtOAc. The combined EtOAc extracts were washed with brine, dried (sodium sulfate), and concentrated. Purification of the residue by flash chromatography gave the desired product (120 mg, 69%) as light yellow foam: MS (ESI) m/z 833.47 (M+H).

To a solution of the above product (120 mg, 0.144 mmol, 1.0 equiv) in THF (10 mL) was added 6 N HCl (1.5 mL) at rt. The resulting mixture was stirred at rt for 2 hrs, diluted with EtOAc, washed with saturated aqueous NaHCO₃ and brine, dried over sodium sulfate, and concentrated. Purification of the residue by flash chromatography gave crude aldehyde S33-6 as a light yellow foam: MS (ESI) m/z 819.40 [(M+MeOH)+H].

Compound S33-6 (45 mg, 0.057 mmol, 1.0 equiv) was dissolved in 1,2-dichloroethane (3.0 mL). Neopentylamine (20 μL, 0.171 mmol, 3.0 equiv) and acetic acid (13.6 μL, 0.228 mmol, 4.0 equiv) were added. After stirring at rt for 1 h, sodium triacetoxyborohydride (24.1 mg, 0.104 mmol, 2.0 equiv) was added. Stirring was continued overnight. The reaction mixture was poured into saturated aqueous NaHCO₃ and extracted three times with dichloromethane. The combined organic extracts were washed with brine, dried over sodium sulfate, and concentrated to give the crude intermediate (20 mg), which was used directly in the next step without further purification: MS (ESI) m/z 858.50 (M+H).

In a plastic vial, the above amine intermediate was dissolved in CH₃CN (1 mL). Aqueous HF (48-50%, 0.25 mL) was added. After stirring at rt for 16 hrs, the reaction mixture was poured into aqueous solution (10 mL) of K₂HPO₄ (2.0 g) and extracted three times with dichloromethane. The combined organic phases were washed with brine, dried, and concentrated to yield the crude intermediate: MS (ESI) m/z 744.40 (M+H).

The above crude intermediate was dissolved in MeOH (2 mL) with 0.5 N HCl/MeOH (0.4 mL). Pd—C (10 wt %, 20 mg) was added. The reaction flask was briefly evacuated and re-filled with hydrogen. The reaction mixture was stirred at rt and monitored by LC-MS. After the reaction was complete, the mixture was filtered through a small pad of Celite. The filtrate was concentrated to give the crude product, which was purified by HPLC on a Waters Autopurification system using a Phenomenex Polymerx 10μ RP-γ 100 R column [30×21.20 mm, 10 micron; flow rate, 20 mL/min; Solvent A: 0.05 N HCl/water; Solvent B: MeOH; injection volume: 4.0 mL (0.05 N HCl/water); gradient: 20→100% B over 10 min; mass-directed fraction collection]. Fractions containing the desired product were collected and freeze-dried to give the desired product as a yellow solid S33-8-1 (4.5 mg, 14.9% for three steps): ¹H NMR (400 MHz, CD₃OD) δ 6.94 (d, J=6.0 Hz, 1H), 4.34 (s, 2H), 4.08 (s, 1H), 3.21-2.92 (m, 3H), 3.03 (s, 3H), 2.96 (s, 3H), 2.92 (s, 2H), 2.65-2.52 (m, 1H), 2.25-2.16 (m, 1H), 1.68-1.55 (m, 1H), 1.06 (s, 9H); MS (ESI) m/z 532.25 (M+H).

The following compounds were prepared according to the methods for compound S33-8-1.

S33-8-2:

¹H NMR (400 MHz, CD₃OD) δ 6.97 (d, J=6.0 Hz, 1H), 4.63-4.54 (m, 1H), 4.40-4.32 (m, 1H), 4.08 (s, 1H), 3.22-2.86 (m, 13H), 2.64-2.53 (m, 1H), 2.24-2.16 (m, 1H), 1.68-1.58 (m, 1H), 1.08 (s, 9H); MS (ESI) m/z 546.28 (M+H).

S33-8-3:

¹H NMR (400 MHz, CD₃OD/DCl) δ 6.92 (d, J=5.96 Hz, 1H), 4.30-4.20 (m, 2H), 4.08 (s, 1H), 3.18-2.94 (m, 3H), 3.04 (s, 3H), 2.96 (s, 3H), 2.90 (dd, J=13.1, 4.6 Hz, 1H), 2.62-2.52 (m, 1H), 2.25-2.14 (m, 1H), 1.68-1.56 (m, 1H), 1.47 (s, 9H); MS (ESI) m/z 518.24 (M+H).

The compounds of the invention including those described above are set forth in Figures: FIGS. 2A-2K; FIGS. 3A-3EE; FIGS. 4A-4Z; FIGS. 5A-5O; FIGS. 6A-6FF.

Example 34. Antibacterial Activity of Compounds of the Invention

The antibacterial activities for the compounds of the invention were studied according to the following protocols.

Minimum Inhibitory Concentration Assay

Frozen bacterial strains were thawed and subcultured onto Mueller Hinton Broth (MHB) or other appropriate media (Streplococcus requires blood and Haemophilus requires hemin and NAD). Following incubation overnight, the strains were subcultured onto Mueller Hinton Agar and again incubated overnight. Colonies were observed for appropriate colony morphology and lack of contamination. Isolated colonies were selected to prepare a starting inoculum equivalent to a 0.5 McFarland standard. The starting inoculum was diluted 1:125 using MHB for further use. Test compounds were prepared by dilution in sterile water to a final concentration of 5.128 mg/mL. Antibiotics (stored frozen, thawed and used within 3 hours of thawing) and compounds were further diluted to the desired working concentrations.

The assays were run as follows. Fifty μL of MHB was added to wells 2-12 of a 96-well plate. One hundred μL of appropriately diluted antibiotics was added to well 1. Fifty μL of antibiotics was removed from well 1 and added to well 2 and the contents of well 2 mixed by pipetting up and down five times. Fifty μL of the mixture in well 2 was removed and added to well 3 and mixed as above. Serial dilutions were continued in the same manner through well 12. Fifty μL was removed from well 12 so that all contained 50 μL. Fifty μL of the working inoculum was then added to all test wells. A growth control well was prepared by adding 50 μL of working inoculum and 50 μL of MHB to an empty well. The plates were then incubated at 37° C. overnight, removed from the incubator and each well was read on a plate reading mirror. The lowest concentration (MIC) of test compound that inhibited the growth of the bacteria was recorded.

Example

1 2 3 4 5 6 7 8 9 10 11 12 [Abt] 32 16 8 4 2 1 0.5 0.25 0.125 0.06 0.03 0.015 Grow − − − − − + + + + + + + [Abt] = antibiotic concentration in the well Grow = bacterial growth (cloudiness)

Interpretation: MIC=2 μg/mL

Protocol for Determining Inoculum Concentration (Viable Count)

Ninety μl of sterile 0.9% NaCl was pipetted into wells 2-6 of a 96-well microtiter plate. Fifty 50 μl of the inoculum was pipetted into well 1. Ten μL from was removed from well 1 and added it to well 2 followed by mixing. Ten μL was removed from well two and mixed with the contents of well 3 and so on creating serial dilutions through well 6. Ten μL was removed from each well and spotted onto an appropriate agar plate. The plate was placed into a CO₂ incubator overnight. The colonies in spots that contain distinct colonies were counted. Viable count was calculated by multiplying the number of colonies by the dilution factor.

Spot from Well 1 2 3 4 5 6 Dilution 10² 10³ 10⁴ 10⁵ 10⁶ 10⁷ Factor Bacterial Strains

Fifteen bacterial strains, listed below, were examined in minimum inhibitory concentration (MIC) assays.

Strain Organism Designation Key Properties Staphylococcus aureus SA100 ATCC 13709, MSSA, Smith strain Staphylococcus aureus SA101 ATCC 29213, CLSI quality control strain, MSSA Staphylococcus aureus SA191 HA-MRSA, tetracycline-resistant, lung infection model isolate Staphylococcus aureus SA161 HA-MRSA, tetracycline-resistant, tet(M) Staphylococcus aureus SA158 Tetracycline-resistan tet(K) aaaureusaureus Staphylococcus SE164 ATCC 1228, CLSI quality control epidermidis strain, tetracycline-resistant Enterococcus faecalis EF103 ATCC 29212, tet-I/R, control strain Enterococcus faecalis EF159 Tetracycline-resistant, tet(M) Enterococcus faecalis EF327 Wound isolate (US) tet(M) Enterococcus faecium EF404 Blood isolate (US) tet(M) Streptococcus SP106 ATCC 49619, CLSI quality control strain pneumoniae Streptococcus SP160 Tetracycline-resistant, tet(M) pneumoniae Streptococcus pyogenes SP312 2009 clinical isolate, tet(M) Streptococcus pyogenes SP193 S. pyogenes for efficacy models; tetS; sensitive to sulfonamides Haemophilus influenzae HI262 Tetracycline-resistant ampicillin- resistant Moraxella catarrhalis MC205 ATCC 8176, CLSI quality control strain Escherichia coli EC107 ATCC 25922, CLSI quality control strain Escherichia coli EC155 Tetracycline-resistant, tet(A) Escherichia coli EC878 MG1655 tolC::kan Escherichia coli EC880 lpxA Escherichia coli EC882 impA Escherichia coli EC200 MDR uropathogenic; serotype O17:K52:H18; UMN 026; trimeth/sulfa- R; BAA-1161 Enterobacter cloacae EC108 ATCC 13047, wt Enterobacter cloacae EC603 Urine isolate (Spain) Klebsiella pneumoniae KP109 ATCC 13883, wt Klebsiella pneumoniae KP153 Tetracycline-resistant, tet(A), MDR, ESBL⁺ Klebsiella pneumoniae KP457 2009 ESBL⁺, CTX-M, OXA Proteus mirabilis PM112 ATCC 35659 Proteus mirabilis PM385 Urine ESBL⁺ isolate Pseudomonas aeruginosa PA111 ATCC 27853, wt, control strain Pseudomonas aeruginosa PA169 Wt, parent of PA170-173 Pseudomonas aeruginosa PA173 PA170 ΔmexX; MexXY-(missing a functional efflux pump) Pseudomonas aeruginosa PA555 ATCC BAA-47, wild type strain PAO1 Pseudomonas aeruginosa PA556 ultiple-Mex efflux pump knockout strain Pseudomonas aeruginosa PA689 Blood isolate (US) Acinetobacter baumannii AB110 ATCC 19606, wt Acinetobacter baumannii AB250 Cystic fibrosis isolate, MDR Stenotrophomonas SM256 Cystic fibrosis isolate, MDR maltophilia Burkholderia cenocepacia BC240 Cystic fibrosis isolate, MDR *MDR, multidrug-resistant; MRSA, methicillin-resistant S. aureus; MSSA, methicillin-sensitive S. aureus; HA-MRSA, hospital-associated MRSA; tet(K), major gram-positive tetracycline efflux mechanism; tet(M), major gram-positive tetracycline ribosome-protection mechanism; ESBL⁺, extended spectrum β-lactamase Results

Values of minimum inhibition concentration (MIC) for the compounds of the invention are provided in FIGS. 7A-7J; FIGS. 8A-8D; FIGS. 9A-9M; FIGS. 10A-10I; FIGS. 11A-11G.

Biological Testing

Neutropenic Respiratory Infection Models for S. pneumoniae

Compounds were tested in a neutropenic BALB/c murine model of lung infection challenged with tetracycline-resistant tet(M) S. pneumoniae strain SP160. Mice were made neutropenic by pre-treatment with cyclophosphamide and infected with SP160 via intranasal administration. Mice were dosed orally with 30 mg/kg compound at 2 and 12 hours post-infection. At 24 hours following initiation of treatment, mice were euthanized and bacterial reduction in the lung was quantified by plating lung homogenates. Data was recorded as log₁₀ reduction in lung colony forming units versus an untreated control group. The results of the testing are shown in Table A.

TABLE A Bacterial Reduction in the Lung (Log₁₀ change from COMPOUND NO. 24 hour control) S2-4-28 −0.06 S15-13-188 −0.22 S15-13-223 −0.12 S16-10-79 −4.31 S24-9-21 −0.89 Neutropenic Respiratory Infection Model for MRSA

Compounds were tested in a neutropenic BALB/c murine model of lung infection challenged with a tetracycline-resistant tet(M) MRSA strain SA191 infected via intranasal administration. At 2 and 12 hours mice were dosed orally with 50 mg/kg compound. At 24 hours following initiation of treatment, mice were euthanized and bacterial reduction in the lung was quantified by plating lung homogenates. Data was recorded as logo reduction in lung colony forming units versus an untreated control group. The results of the testing are shown in Table B.

TABLE B Bacterial Reduction in the Lung (Log₁₀ change from COMPOUND NO. 24 hour control) S29-2-4 −1.29 S16-10-79 −1.27 S24-9-21 −1.98 Mouse Systemic Infection Protocol—S. aureus Smith Septicemia

Compounds were screened for antibacterial activity in vivo in a mouse systemic infection (septicemia) model. In the model, CD-1 female mice (18-22 grams) were injected IP with a S. aureus Smith (ATCC 13709) inoculum that results in 0% survival within 24 to 48 hours. The bacterial dose required to achieve this effect was previously established through virulence studies. At one hour post infection, mice received either 3 mg/ml IV or 30 mg/ml PO. Typically, six mice were treated per dose group. Animal survival was assessed and recorded for 48 hours. Percent survival at 48 hours was recorded and PD₅₀ (mg/kg) was determined for each compound tested. The results (% survival or PD50, the dose in mg/kg that prevents 50% of the mice from death) are presented in Table C.

TABLE C SA Smith SA Smith septicemia survival septicemia IV PO PD50 (mg/kg) Compound (3 mg/kg) 30 mg/kg) IV PO S1-14-105 67% 0% S1-14-128 100% 100% S1-14-134 50% 17% S1-14-25 33% 17% S1-14-28 33% 17% S1-14-31 0% 0% S1-14-36 33% 0% S1-14-4 100% 0% S1-14-46 0% 0% S1-14-73 50% 75% S1-14-90 33% 0% S1-14-91 17% 60% S15-13-117 33% 20% S15-13-15 100% 100% 3.5 S15-13-16 33% 17% S15-13-17 100% 67% S15-13-182 33% 67% S15-13-184 17% 0% S15-13-187 100% 100% S15-13-188 100% 100% 8.3 S15-13-194 100% 100% S15-13-203 100% 100% S15-13-215 50% 50% S15-13-221 33% 50% S15-13-222 100% 100% S15-13-223 100% 100% S15-13-225 100% 100% S15-13-226 100% 83% S15-13-227 17% 0% S15-13-23 83% 50% S15-13-4 100% 100% 6.2 S15-13-5 50% 17% S15-13-8 67% 50% S16-10-107 100% 100% S16-10-11 100% 100% 10 S16-10-161 100% 100% S16-10-177 100% 100% S16-10-18 17% 0% S16-10-79 100% 100% S19-8-1 100% 100% (1) 0.36 12.2 S21-12-1 100% 33 S2-4-11 0% 0% S2-4-12 100% 100% 0.69 2.1 S2-4-14 100% 100% 0.84 12.2 S2-4-19 100% 83% 1 14.3 S2-4-21 16% (2) 0% S2-4-25 100% 100% 0.35 1.4 (2) S2-4-28 100% 100% 0.62 3 S2-4-29 100% 100% 1 3.5 S2-4-30 33% >60 S2-4-33 67% 33% S2-4-41 0% 0% S2-4-50 33% 20% S2-4-50 100% 100% 1 10.1 S2-4-57 50% 33% S2-4-60 17% 33% S2-4-62 17% 25% S2-4-65 100% 100% 8.4 S2-4-66 100% 100% 8.5 S2-4-7 67% 17% S24-9-10 100% 100% S24-9-17 100% 100% S24-9-19 100% 100% S24-9-21 100% 100% S24-9-23 100% 100% S24-9-25 100% 100% S24-9-31 100% 100% S25-11-1 100% 100% S25-11-12 0% 20% S25-11-171 83% 17% S25-11-48 100% 100% S25-11-56 67% 33% S25-11-68 50% 83% S27-9-5 83% 67% S27-9-7 17% 33% S29-2-4 100% 83% S3-5-5 100% 100% S6-4-1 100% 100% Kidney Infection Models for Uropathogenic E. coli EC200 and K. pneumoniae ESBL Isolate KP453

Compounds were tested in a BALB/c murine kidney infection model challenged with tetracycline-resistant E. coli strain uropathogenic ED200 via intravenous injection. At 12 and 24 hours post infection mice were treated orally with 2 mg/kg of test compound. For IV administration test compound was dosed at 3 mg/kg. Thirty-six hours following initiation of treatment, mice were euthanized and bacterial reduction in the kidney was quantified by plating kidney homogenates. Results are reported in Table D as log₁₀ reduction in colony forming units (CFUs) in the kidney versus untreated controls (receiving no test compound).

Compounds were also tested in BALB/c murine kidney infection model challenged with levofloxacin-resistant, ESBL⁺ strain KP453 via intravenous injection with 2% carageenan. At 9 and 24 hours post infection mice were treated orally with 50 mg/kg of test compound. For IV administration, test compounds were dosed at 20 mg/kg. At 36 hours following initiation of treatment, mice were euthanized and bacterial reduction in the kidney was quantified by plating homogenates. Results are reported in Table D as log₁₀ reduction in colony forming units (CFUs) in the kidney versus untreated controls (receiving no test compound).

TABLE D KP453 ESBL KP453 EC200 UTI EC200 UTI UTI ESBL UTI Compd. No. IV 3 mg/kg PO 2 mg/kg IV 20 mg/kg PO 50 mg/kg S2-4-25 −3.63 @ −1.07, 3 mpk; −2.15^(a) −2.78 A 2 mpk S2-4-19 −1.91 −1.27 S2-4-12 −2.51 −2.15 S2-4-65 −4.61 @ −2.28 12 mpk; −3.18 @ 2 mpk S3-5-5 −2.26 −1.01, −1.74^(a) S15-13-15 −2.82 −1.45 S15-13-4 −3.17 −1.69 S15-13-188 −3.48 @ −1.55 3 mpk S15-13-187 −3.46 @ −0.98, −1.99^(a) 3 mpk S15-13-194 −3.44 −2.18 S15-13-122 −2.64 −1.84 S15-13-223 −2.27 −1.32 S16-10-161 @5 mpk −1.85, −3.09 −1.04, −1.07^(a) −3.99 −2.83 S24-9-17 −2.61 −2.41 S24-9-23 @ 5 mpk −2.99 −2.95 −2.93 −4.34 S24-9-21 @ 5 mpk −2.30 −2.87 −2.16 −4.09 S24-9-25 −2.99 −1.71 S25-11-1 −3.97 −1.84 ^(a)Different testing days Neutropenic Thigh Model

Female CD-1 mice were pre-treated with cyclophosphamide to render the mice neutropenic. Mice were infected with S. aureus ATCC 13709 via injection of 0.1 ml into the right thigh muscle of each mouse. One and a half hours post infection mice were treated IV with test compounds in doses ranging from 0.3 to 30 mg/kg or 0.3 to 20 mg/kg. Four mice were treated with each drug concentration. Twenty-four hours post treatment, mice were euthanized by CO₂ inhalation. The right thighs of the mice were aseptically removed, weighed, homogenized, serially diluted, and plated on TSA medium. The plates were incubated overnight at 37° C. in 5% CO₂. Colony forming units per gram of thigh was calculated by enumerating the plated colonies then adjusting for serial dilutions and the weight of the thigh. Data was recorded as log₁₀ reduction in colony forming units versus an untreated control group and is presented in Table E.

TABLE E COMPOUND NO. log₁₀ REDUCTION S2-4-14 3.68 S2-4-25 4.1 22-4-29 4.35 Pharmacokinetic Studies in Rats

Test compounds were evaluated in fasted (no food for 18 hours prior to dosing) male Sprague Dawley rats (3 animals per group) by administration of 1 mg/kg intravenously into the jugular vein and 10 mg/jg by oral gavage. Ten plasma samples were drawn for each dosing route up to 24 hours into heparin-coated vacutainer tubes. The plasma concentrations of the test compounds were quantified by LC/MS/MS using an internal standards. WinNONLIN was used to determine PK parameters±standard deviation (AUC, Cmax, CL, Vss and % oral bioavailability (% F) and are set forth in Table F.

TABLE F Oral Rat PK Cmpd. No. 10 mg/kg IV Rat PK 1 mg/kg S2-4-14 Cmax 543; AUC 6160; 13.7% Cmax 667; AUC (% F) 4432; Cl 233; Vss 1.4 S2-4-25 Cmax 76.7; AUC 826; 9.1% Cmax 214; AUC (% F) 838; Cl 1164; Vss 6.6 12 h urine 11 ug/mL S2-4-28 Cmax 381; AUC 4381; 44.7% Cmax 170; AUC (% F) 970; Cl 1056; Vss 5.7; S2-4-29 Cmax 171; AUC 1597; 25.4% Cmax 112; AUC (% F) 616; Cl 1589; Vss 9.2 S2-4-19 Cmax 572; AUC 4981; 14.9% Cmax 518; AUC (% F) 3321; Cl 300; Vdss 1.6 S2-4-12 Cmax 117; AUC 1163; 14.6% Cmax 126; AUC (% F) 754; Cl 1277; Vdss 9.3 S15-13-15 Cmax 162; AUC 2263; 21.0% Cmax 154; AUC (% F) 1104; Cl 927; Vdss 6.4 S16-10-161 Cmax 465; AUC 4442; 31.6% Cmax 689; AUC (% F) 1307; Cl 768; Vdss 4.96 S24-9-23 Cmax 310; AUC 2226; 12.2% Cmax 795; AUC (% F) 1831; Cl 555.6; Vdss 3.9 SA MIC90

FIGS. 1A-1C provides the results of testing selected compounds against selected groups isolates as indicated in the figure to determine the minimal inhibitory concentration (MIC) of test compounds for 90% of the isolates (MIC₉₀). MICs were performed by microtiter broth dilution in a 96-well format according to Clinical Laboratory Standards Institute (CLSI) guidelines, as described above.

Viable counts were determined by 10-fold serial dilution. Dilutions were prepared in sterile 0.9% NaCl. Ten microliters of the inoculum and of each of 5 dilutions were plated onto blood or Mueller Hinton agar plates, incubated overnight at 37° C. with 5% CO₂, and counted. Results are shown in FIGS. 1A-1C.

Monkey PK

Compounds S24-9-23 and S16-10-161 were evaluated in 3 non-naïve cynomolgus monkeys. Each animal received a single IV dose of 1 mg/kg and after a 7-day washout, received a single PO dose of 10 mg/kg. Nine to ten plasma samples were drawn for each dosing route up to 24 hours into heparin-coated vacutainer tubes. Dose formulations were verified with a 5-point calibration curve. The plasma concentration of the compound was quantified by LC/MS/MS using an internal standard. WinNonLin was used to determine individual and mean PK parameters±standard deviation (% F (oral bioavailability), Cmax, Tmax, CL, Vss, AUC. Results were as follows: For S24-9-23 oral: Cmax=1350 ng/mL; AUC=22767 ng·hr/mL; % F=36.8% and IV: Cmax=1373 ng/mL; AUC=7070 ng·h/mL; Cl=144 mL/hr/kg, Vdss=3.4 L/kg. For S16-10-161 oral: Cmax=982 ng/mL; AUC=26000 ng·hr/mL; % F=42.7% and IV: Cmax=861; AUC=5677 ng·h/mL; Cl=183.6 mL/hr; Vdss=4.047.

While this invention has been particularly shown and described with references to example embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention encompassed by the appended claims. 

What is claimed is:
 1. A compound of Formula (II):

or a pharmaceutically acceptable salt thereof, wherein: X is selected from fluoro or chloro; R^(1a) is hydrogen; and R² and R³ taken together with the nitrogen atom to which they are bound form a (4-7 membered) monocyclic heterocylic ring, or a (6-13 membered) bicyclic heterocylic ring, wherein the (4-7 membered) monocyclic heterocylic ring or the (6-13 membered) bicyclic heterocyclic ring is optionally substituted with one or more substituents independently selected from C₃-C₁₀ carbocyclyl, (4-13 membered) heterocyclyl, fluoro, chloro, —OH, —C₁-C₄ fluoroalkyl, —C₁-C₄ alkyl, —O—C₃-C₁₀ carbocyclyl, —O-(4-13 membered) heterocyclyl, —C₀-C₄ alkylene-O—C₁-C₄ alkyl, —C₀-C₄ alkylene-O—C₁-C₄ fluoroalkyl, ═O, —C(O)—C₁-C₄ alkyl, —C(O)N(R⁴)(R⁵), —N(R⁴)—C(O)—C₁-C₄ alkyl, and —C₀-C₄ alkylene-N(R⁴)(R⁵), and wherein each carbocyclyl or heterocyclyl substituent is optionally substituted with fluoro, chloro, —OH, C₁-C₄ fluoroalkyl, C₁-C₄ alkyl, —O—C₁-C₄ alkyl, —O—C₁-C₄ fluoroalkyl, —NH₂, —NH(C₁-C₄ alkyl), or —N(C₁-C₄ alkyl)₂; and each of R⁴ and R⁵ is independently selected from hydrogen and C₁-C₄ alkyl; or R⁴ and R⁵ taken together with the nitrogen atom to which they are bound form a (4-7 membered) heterocylic ring optionally comprising one additional heteroatom selected from N, S and O, wherein the (4-7 membered) heterocylic ring is optionally substituted with fluoro, chloro, —OH, fluoro-substituted C₁-C₄ alkyl, —C₁-C₄ alkyl, or —C₁-C₄ alkylene-O—C₁-C₄ alkyl, and is optionally fused to phenyl.
 2. The compound of claim 1, wherein: R² and R³ taken together with the nitrogen atom to which they are bound form a ring selected from pyrrolidine, piperidine, piperazine or morpholine, wherein the ring is optionally substituted with one or more substituents independently selected from fluoro, —OH, —C₁-C₃ alkyl and —C₁-C₃ alkylene-O—C₁-C₃ alkyl, and wherein the ring is optionally fused to phenyl or spirofused to cyclopropyl.
 3. The compound of claim 2, wherein: R² and R³ taken together with the nitrogen atom to which they are bound form a ring selected from pyrrolidine and piperidine.
 4. The compound of claim 3, wherein X is chloro.
 5. The compound of claim 4, wherein: R² and R³ taken together with the nitrogen atom to which they are bound form a ring selected from pyrrolidine and piperidine, wherein the ring is fused to phenyl.
 6. The compound of claim 5, represented by the following structural formula:

or a pharmaceutically acceptable salt thereof.
 7. The compound of claim 1, wherein: the (4-7 membered) monocyclic heterocylic ring, or a (6-13 membered) bicyclic heterocylic ring, wherein the (4-7 membered) monocyclic heterocylic ring, or the (6-13 membered) bicyclic heterocyclic ring is substituted with at least one substituent independently selected from C₃-C₁₀ carbocyclyl, (4-13 membered) heterocyclyl, and is optionally substituted with one or more substituents independently selected from fluoro, —OH, —C₁-C₃ alkyl and —C₁-C₃ alkylene-O—C₁-C₃ alkyl.
 8. The compound of claim 7, wherein: the R² and R³ taken together with the nitrogen atom to which they are bound form a ring selected from pyrrolidine, piperidine, piperazine or morpholine.
 9. The compound of claim 8, wherein: the C₃-C₁₀ carbocyclyl is a phenyl.
 10. The compound of claim 9, wherein R² and R³ taken together with the nitrogen atom to which they are bound form a ring selected from pyrrolidine and piperidine.
 11. The compound of claim 10, wherein X is fluoro.
 12. The compound of claim 11, represented by the following structural formula,

or a pharmaceutically acceptable salt thereof.
 13. A pharmaceutical composition comprising a pharmaceutically acceptable carrier or diluent and a compound of claim
 1. 14. A method for ameliorating the clinical symptoms of cancer in a subject in need thereof, comprising administering to the subject an effective amount of the composition of claim 13, wherein the cancer is colon cancer, ovarian cancer, or kidney cancer.
 15. A method for ameliorating the clinical symptoms of cancer in a subject in need thereof, comprising administering to the subject an effective amount of the compound of claim 1 or a pharmaceutically acceptable salt thereof, wherein the cancer is colon cancer, ovarian cancer, or kidney cancer. 